Asian Pacific Journal of Cancer Prevention最新文献

{"title":"Recent Advances in Cancer Immunotherapy Aided by Regulatory Non-Coding RNA: A Review.","authors":"Debabrat Baishya, Arpita Barman, Bijuli Rabha, Kaushik Kumar Bharadwaj, Joyeeta Talukdar","doi":"10.31557/APJCP.2025.26.6.1915","DOIUrl":"10.31557/APJCP.2025.26.6.1915","url":null,"abstract":"<p><p>Cancer immunotherapies have remodeled the way many different forms of cancer are treated by, leveraging the immune system to recognize and attack malignant cells. These therapies have demonstrated durable responses in cancers affecting the blood, bone marrow, and lymph nodes, solidifying their role as a cornerstone of modern oncology. However, despite their success, current immunotherapy tools and techniques require further refinement.Regulatory non-coding RNAs (ncRNAs), actively transcribed by the mammalian genome, play crucial roles in regulating a wide range of cellular processes. They are key players in epigenetic mechanisms that govern differentiation, development, and the progression of invasive carcinogenesis. Emerging evidence suggests that regulatory ncRNAs are also pivotal in cancer immunity, acting as drivers of immune responses in the development of metastatic tumors. These ncRNAs influence the tumor microenvironment (TME), modulate the immune system, and affect the efficacy of immune checkpoint inhibitors and T cell therapies by regulating various signaling pathways.This review article explores the diagnostic and therapeutic potential of ncRNAs in cancer immunotherapy, highlighting their role in modulating carcinogenesis and antitumor immunity. By understanding the influence of regulatory ncRNAs, we can pave the way for the development of prognostic biomarkers and more effective tumor immunotherapies.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"1915-1930"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scored Patient-Generated Subjective Global Assessment (PG-SGA) in Brazilian Cancer Patients and Association with Anthropometric Parameters.","authors":"Odara Maria De Sousa Sa, Ana Karoline Ferreira Dos Santos, Thayssa Lauanna Vitória E Silva Santos, Izabella Fontenelle Menezes Freitas, Ana Karoline Sousa Soares Leal, Maria Jose Santos, Tonny Kerley Rodrigues, Danilo Carvalho Oliveira","doi":"10.31557/APJCP.2025.26.6.2009","DOIUrl":"10.31557/APJCP.2025.26.6.2009","url":null,"abstract":"<p><strong>Introduction: </strong>The Scored Patient-Generated Subjective Global Assessment (PG-SGA) is a multidimensional tool used to assess malnutrition and associated risk factors.</p><p><strong>Objective: </strong>To evaluate the nutritional status of Brazilian cancer patients hospitalized using the Scored PG-SGA and to examine the correlations with selected nutritional parameters.</p><p><strong>Methods: </strong>This observational study included 2.027 cancer patients aged over 18 years at a hospital of cancer, Brazil. All patients were assessed for nutritional status using the Brazilian PG-SGA and anthropometric measurements were evaluated.</p><p><strong>Results: </strong>According to the PG-SGA global assessment categories, 56.2% (1.138) cancer patients were well-nourished, 29.3% (594) were moderately malnourished, and 14.4% (292) were severely malnourished. The average body mass index (BMI) was 23.7±5.49 kg/m², and the current weight was 66.34±58.07 kg. The PG-SGA, nutritional status and clinical variable assessed by PG-SGA, were all significantly correlated with body mass index (p < 0.005) and weigh < 0.005).</p><p><strong>Conclusions: </strong>The Brazilian PG-SGA showed a 43.7% of malnourished cancer patients. The classification of severely malnourished by PG-SGA demonstrated strong positive correlations with BMI. Anthropometric parameters correlates with PG-SGA variable, weigh with sex, nausea, diarrhea, dysgeusia, diagnostics of cancer, fever and death and BMI with sex, food intake, nausea, diarrhea , dysgeusia.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2009-2014"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Group CBT for Depression in Newly Diagnosed Breast Cancer Patients in a Malaysian Public Hospital.","authors":"Nur Haidzat Abd Wahid, Normala Ibrahim, Zubaidah Jamil Osman, Nor Aina Emran, David James De L Horne, Siti Irma Fadhilah Ismail","doi":"10.31557/APJCP.2025.26.6.1971","DOIUrl":"10.31557/APJCP.2025.26.6.1971","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer stays as one of the most prevalent cancers worldwide, and the increasing number of cases alerts the urgent need for comprehensive support for affected patients. Breast cancer surgery, such as mastectomy, often induces significant psychological distress, including depression, that is particularly pronounced in newly diagnosed patients. The study aims to evaluate the presence of depressive symptoms in newly diagnosed breast cancer patients undergoing mastectomy and assess the effectiveness of group cognitive behaviour therapy (G-CBT) in reducing depression.</p><p><strong>Methods: </strong>An experimental study was conducted at a large tertiary public hospital in Kuala Lumpur. Seventy (70) newly diagnosed breast cancer patients were recruited and randomly allocated to an intervention (G-CBT) or control group. The PHQ-9 questionnaire was used to assess the level of depressive symptoms at baseline, immediate post-operation, and post operation in the first and third-month follow-up. A mixed design ANOVA analysis was used to determine the effect of G-CBT on depressive symptoms with partial eta squared as a measure of the intervention effect size.</p><p><strong>Results: </strong>The study found that both groups showed significant depressive symptoms above the threshold for clinical attention prior to mastectomy. The evaluation of the effect of G-CBT across time points revealed a general reduction in depressive symptoms for both groups. However, subjects in the G-CBT intervention group showed a significantly greater reduction in depressive symptoms compared to the control group. The effect of G-CBT on depressive symptom scores sustained until the third month after mastectomy and proved that the psychological intervention is effective in alleviating psychological distress.</p><p><strong>Conclusion: </strong>G-CBT significantly reduced depressive symptoms in newly diagnosed breast cancer patients and the effect of intervention sustains until third month follow-up. These findings emphasize the value of incorporating G-CBT into standard care practices to provide targeted psychological support and improve mental health outcomes in this vulnerable population.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"1971-1976"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Accuracy of Plate-Based Digital PCR Assays for Simultaneous Quantitation of Hepatitis B and D Viral Loads in Patients' Serum.","authors":"Thao Thi-Thu Huynh, Anh Thi Ha, Nga Thi Nguyen, Yen Thi-Hai Vu, Danh Hoang Nguyen, Tuan Manh Ha, Anh Tuan Nguyen","doi":"10.31557/APJCP.2025.26.6.2079","DOIUrl":"10.31557/APJCP.2025.26.6.2079","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to evaluate the diagnostic accuracy of plate-based digital PCR assays that will accurately and sensitively measure HBV and HDV viral loads in patients' serum simultaneously.</p><p><strong>Methods: </strong>First, we optimized the components and parameters for real-time PCR and RT-PCR assays to measure HBV and HDV levels. Next, we employed identical components and parameters in digital PCR and digital RT-PCR assays. Subsequently, we evaluated the accuracy and sensitivity of these assays compared to established techniques and assessed their efficacy by analyzing different serum samples.</p><p><strong>Results: </strong>The technical sensitivity of the optimized digital PCR procedures was 1-5 copies/reaction for both HBV and HDV. Compared to real-time PCR and real-time RT-PCR assays, HBV and HDV quantitative digital PCR assays demonstrated strong correlation coefficients (R2HBV = 0.944 and R2HDV = 0.900). Substantial levels of agreement were indicated by Lin's Concordance Coefficient (CCCHBV = 0.963 and CCCHDV = 0.933). In addition, 2% (2/98) of the clinical samples had low HBV concentrations that the digital PCR could identify but not the real-time PCR. The HDV prevalence found in the study was 3.1% (3/98) by real-time PCR but 5.1% (5/98) by digital PCR.</p><p><strong>Conclusion: </strong>In summary, the optimized digital PCR assays successfully measured hepatitis B and hepatitis D viruses in serum samples with low viral loads that real-time PCR assays could not detect.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2079-2086"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myths and Misconceptions of Breast Cancer Causation among Female Population of Saudi Arabia.","authors":"Duaa Salem Alkhayat, Zakaria Yahya Khawaji, Mohammed Abdullah Alahmadi, Nussaiba Yahya Khawaji, Hanan Mohammed Alsayed Alrefaei, Lojain Nasser J Alruwaili, Lujain Hamdan Alghamdi","doi":"10.31557/APJCP.2025.26.6.2155","DOIUrl":"10.31557/APJCP.2025.26.6.2155","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer is the most commonly diagnosed malignancy among women in Saudi Arabia, yet public awareness remains limited. Given the significant influence of health beliefs on behavior, this study investigated the prevalence of myths surrounding breast cancer causation in the Western region of Saudi Arabia.</p><p><strong>Methods: </strong>A cross-sectional study was conducted using the modified Cancer Awareness Measure Mythical Causes Scale, a validated tool assessing misconceptions about breast cancer. Participants working in healthcare were excluded to better capture public perceptions. An online questionnaire collected socio-demographic data and assessed breast cancer myths. Univariate and multivariate logistic regression analyses assessed the associations between knowledge and socio-demographic factors, with p-values ≤ 0.05 considered statistically significant.</p><p><strong>Results: </strong>470 adult women were included in the study. Only 13.2% demonstrated a good level of awareness, correctly identifying ten or more myths. In univariate analysis, young (p = 0.02), single (p = 0.01), and unemployed (p = 0.01) women or knowing someone with breast cancer (p = 0.04) were associated with higher awareness of breast cancer myths. Commonly unrecognized myths included psychological stress (63%), exposure to electromagnetic frequencies (47.7%), food additives or artificial sweeteners (46.8%), living in industrial cities (46.4%), and undergoing breast plastic surgery (46.2%). Notably, women with lower recognition of myths were more likely to believe mammograms could cause breast cancer (p < 0.001).</p><p><strong>Conclusion: </strong>This study highlights widespread misconceptions about breast cancer causation among our sample. Educational programs are essential to debunk myths and foster evidence-based awareness in clinical and public environments.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2155-2162"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Laughter Yoga on Chemotherapy-Induced Symptoms and Sleep Quality in Patients with Haematologic Cancer.","authors":"Ozlem Ugur, Gulsah Bedez, Solmaz Zeybekci, Ezgi Karadag","doi":"10.31557/APJCP.2025.26.6.1959","DOIUrl":"10.31557/APJCP.2025.26.6.1959","url":null,"abstract":"<p><p>There are international studies that examine the effects of laughter yoga on the symptoms and quality of life of cancer patients as a complementary therapeutic method.</p><p><strong>Aim: </strong>This study aims to examine the effect of laughter yoga on chemotherapy-induced symptoms and sleep quality in patients with haematologic cancer.</p><p><strong>Materials and methods: </strong>This study was conducted as a randomised controlled trial with two parallel treatment arms. The study was between November 2020 and December 2021. The sample was calculated with 60 patients including in the study. A total of 4 sessions of laughter yoga were applied in the experimental group for 45 minutes twice a week for two weeks. No intervention was applied to the control group; clinical routine care continued.</p><p><strong>Results: </strong>Pittsburgh Sleep Quality Index scores was analysed, a statistically significant difference was found between the scores of the intervention and control groups in the subjective sleep quality component. A significant difference was found between the scores of the intervention group in the sleep duration and sleep disturbances components. Based on the results from the Edmonton Symptom Assessment System, a statistically significant difference was found in the scores of pain and anxiety components in the intervention group, in the scores of tiredness/nausea/depression/drowsiness/appetite/well-being components in the intervention and control groups.</p><p><strong>Conclusion: </strong>A significant difference was found in the scores of the pain and anxiety components in the intervention group. It was also found that the scores of the sleep duration and sleep disturbances components in the intervention group were better than the control group.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"1959-1970"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of XRCC1 Gene Polymorphism with an Increased Risk of Hepatocellular Carcinoma in the Peshawar Population.","authors":"Rashid Saeed Abdo Hassen, Wahby Mohammed Babaresh, Irshad Ur Rehman, Zaid Talal, Maria Nayyar, Yazid Rettas, Syeda Javeria Shakeel, Sawaira Huriya, Aiman Begum, Oyoon Khan","doi":"10.31557/APJCP.2025.26.6.2109","DOIUrl":"10.31557/APJCP.2025.26.6.2109","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to examine the association between variations in the XRCC1 gene and the risk of hepatocellular carcinoma in individuals from Peshawar with chronic hepatitis C infection.</p><p><strong>Methods: </strong>The study included three groups of participants: 30 individuals with HCC resulting from chronic HCV infection, 30 participants with HCV-related cirrhosis, and 30 healthy controls. Detailed clinical and laboratory data were collected for all participants. The ARMS-PCR (Amplification Refractory Mutation System-Polymerase Chain Reaction) method was utilized to identify a single nucleotide polymorphism (SNP) in the XRCC1 gene.</p><p><strong>Result: </strong>The analysis revealed that HCC patients had a significantly higher occurrence of XRCC1 (AA and GA) genotypes and an increased frequency of the A allele compared to both cirrhotic HCV patients and healthy controls. Additionally, individuals with the XRCC1 (AA and GA) genotypes demonstrated greater tumor foci and larger tumor sizes. Multivariate analysis revealed that the XRCC1 rs25487 polymorphism independently contributes to a higher likelihood of developing HCC in patients with chronic HCV, with a 2.66-fold.</p><p><strong>Conclusion: </strong>The polymorphism of the XRCC1 gene is probably linked with an elevated risk of hepatocellular carcinoma in individuals with chronic HCV infection. These findings underscore the importance of genetic factors in assessing HCC risk.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2109-2115"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Potential of a Novel Oil Blend Therapy for Immunity Enhancement and Cervical Carcinoma Treatment.","authors":"Momir Dunjic, Stefano Turini, Lazar Nejkovic, Aleksandra Pikula, Nenad Sulovic, Sasa Cvetkovic, Marija Dunjic, Katarina Dunjic","doi":"10.31557/APJCP.2025.26.6.2129","DOIUrl":"10.31557/APJCP.2025.26.6.2129","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic potential of a novel vegetable oil blend containing natural bioactive compounds for the treatment of cervical carcinoma through in silico molecular docking analysis.</p><p><strong>Methods: </strong>Natural compounds were extracted from cold-pressed pumpkin oil (Curcubita maxima), horsetail oil (Equisetum arvense), and etheric clove oil (Syzygium aromaticum). Major phytochemicals quercetin 3-O-glucoside, γ-tocopherol, apigenin 5-O-glucoside, β-caryophyllene, kaempferol 3-O-glycoside, and EGCG were identified and standardized via HPLC. Molecular docking was performed using 1-Click Docking software to assess binding affinities against cervical carcinoma-associated targets (p16INK4a, Ki-67, VEGF, CEA, MMP-9, TP53, and pRb). Docking scores were expressed as Gibbs free energy (ΔG, Kcal/mol). Comparative analyses were conducted versus conventional agents (Paclitaxel, Pembrolizumab, Temsirolimus). AI-assisted optimization using ChatGPT-4o integrated molecular interaction data from over 10,000 peer-reviewed studies.</p><p><strong>Results: </strong>Apigenin 5-O-glucoside showed the strongest interaction with MMP-9 (-11.6 Kcal/mol) and CEA (-9.4 Kcal/mol). Quercetin 3-O-glucoside exhibited high affinity for TP53 (-8.1 Kcal/mol), Ki-67 (-9.1 Kcal/mol), and VEGF (-8.7 Kcal/mol). Natural compounds consistently outperformed standard chemotherapeutics, e.g., Paclitaxel with p16INK4a (-5.4 Kcal/mol) vs. apigenin 5-O-glucoside (-8.9 Kcal/mol). These results suggest robust multi-targeted anticancer potential, including inhibition of proliferation, angiogenesis, and metastasis, along with apoptosis induction.</p><p><strong>Conclusion: </strong>Natural compounds derived from a novel vegetable oil blend demonstrate promising molecular interactions with key biomarkers of cervical carcinoma. These findings support their potential role as effective therapeutic agents and warrant further in vitro and in vivo validation.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2129-2136"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Associated with Attending to the Colorectal Cancer Screening Services among at-Risk Individuals in a Community Care Hospital: A Cross-Sectional Analytical Study.","authors":"Annapha Boonna, Nattakan Choochuay, Bhunyabhadh Chaimay, Somkiattiyos Woradet, Sirichai Changkaew, Kriangsak Sukmai, Supaporn Chukraithai","doi":"10.31557/APJCP.2025.26.6.2163","DOIUrl":"10.31557/APJCP.2025.26.6.2163","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer (CRC) is a major global public health concern, yet screening participation remains low among at-risk populations. This study investigated factors influencing attendance at CRC screening services among at-risk individuals aged 50-70 years.</p><p><strong>Methods: </strong>A cross-sectional analytical study was conducted from November 2023 to February 2024, involving 360 participants aged 50-70 years. The sample included 180 cases (participants who attended CRC screening) and 180 controls (those who did not), selected through simple random sampling from a registry of at-risk individuals. Data were collected using validated questionnaires assessing demographic characteristics, health literacy, social, and family support. The content validity indices ranging from 0.66 to 1.00 with Cronbach's alpha coefficients of 0.91, 0.88, and 0.77, respectively. Descriptive statistics and multiple logistic regression were used for analysis.</p><p><strong>Results: </strong>Annual physical check-ups and body mass index (BMI) were significantly associated with screening attendance with CRC screening attendance. Individuals without annual check-ups were 55% less likely to attend CRC screening (Adjusted Odds Ratio [AOR] = 0.45, 95% Confidence Interval [CI]: 0.29-0.70). Those with a normal BMI were 3.91 times more likely to attend screening compared to those with underweight BMI (AOR = 3.91, 95% CI: 1.20-12.77), after adjusting for gastrointestinal disease history.</p><p><strong>Conclusions: </strong>Regular health check-ups and BMI play crucial roles in CRC screening participation. Targeted health interventions are essential to improve awareness and encourage screening participation, particularly among individuals lacking routine check-ups. Strengthening preventive healthcare efforts can help reduce CRC prevalence and enhance public health outcomes.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2163-2173"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Cytotoxic Impact of Linagliptin - Ciprofloxacin Combination on Cervical Cancer Cell Line: Insights into Targeting Heat Shock Protein 60.","authors":"Tiba Th Al-Mahdawi, Ali Muafaq Said, Istikrar M Hade, Youssef Shakuri Yasin, Azal Hamoody Jumaa","doi":"10.31557/APJCP.2025.26.6.2117","DOIUrl":"10.31557/APJCP.2025.26.6.2117","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to assess the combined impact of linagliptin and ciprofloxacin on inhibiting cervical cancer cell line proliferation and their ability to target heat shock protein 60.</p><p><strong>Methods: </strong>The anticancer properties of the linagliptin-ciprofloxacin combination were assessed employing the HeLa cervical cancer cell line, with incubation periods of 24 and 72 hours. The human fibroblast cell line (HFF) was utilized to evaluate the mixture's safety. The concentrations of linagliptin, ciprofloxacin, and their combination varied between 0.1 and 1000 µg/ml. combination index value was estimated to assess the potential synergistic impact of linagliptin and ciprofloxacin. The study also employs computational molecular docking simulations to evaluate the affinity of linagliptin and ciprofloxacin for binding to heat shock protein 60.</p><p><strong>Results: </strong>The study's findings demonstrated that the combination of linagliptin and ciprofloxacin markedly inhibited the proliferation of cervical cancer cells. The inhibitory effect depended on the concentration of the mixture and the incubation duration. It concurrently exhibits a diminished impact on the viability of the HFF cell line. The combination index study indicates that the interaction between linagliptin and ciprofloxacin shows a synergistic effect across all concentrations, particularly after 24 hours of incubation. The computational molecular docking simulation demonstrated that linagliptin and ciprofloxacin can bind with Hsp 60. The docking scores for linagliptin and ciprofloxacin were recorded at -7.6 kcal/mol and -8.1 kcal/mol, respectively.</p><p><strong>Conclusion: </strong>Our study findings from the MTT assay, combination index, and computational docking simulations indicate that the combination of linagliptin and ciprofloxacin presents a promising option for treating cervical cancer, considering their defined adverse effects and pharmacokinetic profiles.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2117-2128"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}