Methylation of Tumor Suppressor Genes on Chromosome 9: Diagnostic Insights from a Nasopharyngeal Carcinoma in Vietnam.

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-06-01 DOI:10.31557/APJCP.2025.26.6.2239

Hue Hong Thieu, Thuy Ai Huyen Le, Thuan Duc Lao

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引用次数: 0

Abstract

Background: Methylation represents the second hit in tumor development. In nasopharyngeal carcinoma (NPC), multiple tumor suppressor genes located on chromosome 9 under methylation.

Aims: This first case-control study aims to explore the methylation characteristics of DAPK, p16INK4α, p15INK4α, and p14ARF, individually and in combination, to evaluate their potential as promising biomarkers for nasopharyngeal carcinoma (NPC).

Materials and methods: A total of 70 NPC biopsy samples and 60 non-cancerous swab samples were enrolled into the current study. Genomic DNA extraction, bisulfite modification, and methylation-specific PCR (MSP) were used to analyze methylation status. Statistical analyses, including odds ratio (OR) calculations and methylation index (MI) evaluation, were conducted to determine the association between methylation and nasopharyngeal carcinoma (NPC).

Results: Promoter hypermethylation was observed in 75.71%, 78.57%, 52.86%, and 87.14% of samples for DAPK, p16INK4α, p15INK4α, and p14ARF, respectively. A significant association between the methylation status of these candidate genes and nasopharyngeal carcinoma (NPC) was identified. The methylation of these genes significantly increased the risk of NPC compared to control samples (OR > 1). Based on the methylation index (MI), 100% of NPC samples showed methylation in at least one gene (MI ≥ 0.25). An MI ≥ 0.25 demonstrated a sensitivity of 100% and a specificity of 50%, highlighting its potential as a diagnostic marker.

Conclusion: This study highlights the potential of DAPK, p16INK4α, p15INK4α, and p14ARF methylation as biomarkers for the diagnosis and early screening of nasopharyngeal carcinoma (NPC) in Vietnamese patients. The findings support the use of a composite methylation index as a sensitive biomarker for the early detection and screening of NPC.

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9号染色体肿瘤抑制基因的甲基化：越南鼻咽癌的诊断见解。

背景：甲基化是肿瘤发展的第二次打击。在鼻咽癌（NPC）中，位于9号染色体上的多个肿瘤抑制基因甲基化。目的：本研究旨在探讨DAPK、p16INK4α、p15INK4α和p14ARF的甲基化特征，以评估它们作为鼻咽癌（NPC）生物标志物的潜力。材料与方法：本研究共纳入70例鼻咽癌活检样本和60例非癌性拭子样本。基因组DNA提取、亚硫酸盐修饰和甲基化特异性PCR （MSP）用于分析甲基化状态。统计分析包括优势比（OR）计算和甲基化指数（MI）评估，以确定甲基化与鼻咽癌（NPC）之间的关系。结果：DAPK、p16INK4α、p15INK4α和p14ARF的启动子高甲基化率分别为75.71%、78.57%、52.86%和87.14%。这些候选基因的甲基化状态与鼻咽癌（NPC）之间存在显著关联。与对照样本相比，这些基因的甲基化显著增加了鼻咽癌的风险（OR bbb1）。根据甲基化指数（MI）， 100%的鼻咽癌样本至少有一个基因甲基化（MI≥0.25）。MI≥0.25表明其敏感性为100%，特异性为50%，突出了其作为诊断标志物的潜力。结论：该研究强调了DAPK、p16INK4α、p15INK4α和p14ARF甲基化作为越南鼻咽癌（NPC）患者诊断和早期筛查的生物标志物的潜力。这些发现支持使用复合甲基化指数作为鼻咽癌早期检测和筛查的敏感生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.