The Anticancer Effect of Haematococcus Pluvialis Alga Extract on Breast Cancer Cell Line.

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-06-01 DOI:10.31557/APJCP.2025.26.6.2071

Vahideh Shamskhani, Habib Zarredar, Jafar Razeghi, Atefeh Ansarin, Milad Asadi, Zahra Soleimani, Ensiyeh Seyedrezazadeh

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引用次数: 0

Abstract

Objective: The aim of this study was to investigate the anticancer effects of H. pluvialis extract on breast cancer. Methods: We evaluated the cytotoxicity, suppression of migration, and apoptosis ability of H. pluvialis alga extract MDA-MB-468 by MTT assay, scratch test, and flow cytometry, respectively. The expression patterns of MMP1 and MMP9, Bax and Bcl2, Caspase3, Caspase6, and Caspase9 were analyzed by qRT-PCR.

Results: Based on the findings of this study, the survival and proliferation capacity of MDA-MB-468 cells treated with the extract was reduced. Flow cytometry results showed that treatment with H. Pluvialis extracts induced apoptosis. Scratch test results showed that H. Pluvialis extract could inhibit cell migration. The expression patterns of the studied genes were changed by treatment with the extract. Expression of Bcl2, MMP1, and MMP9 genes decreased, and expression of Bax, Caspase3, Caspase8, Caspase9 genes increased.

Conclusion: The findings of this research reveal that H. pluvialis algae extract shows a potential anti-cancer compound.

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雨红球菌藻提取物对乳腺癌细胞株的抗癌作用。

目的：探讨雨柳提取物对乳腺癌的抗癌作用。方法：采用MTT法、划痕法和流式细胞术分别评价雨藻提取物MDA-MB-468的细胞毒性、对迁移的抑制作用和细胞凋亡能力。采用qRT-PCR分析MMP1和MMP9、Bax和Bcl2、Caspase3、Caspase6和Caspase9的表达模式。结果：根据本研究结果，MDA-MB-468细胞的存活和增殖能力降低。流式细胞术结果显示，水仙提取物可诱导细胞凋亡。刮擦试验结果表明，雨竹提取物对细胞迁移有抑制作用。经提取物处理后，所研究基因的表达模式发生了变化。Bcl2、MMP1、MMP9基因表达降低，Bax、Caspase3、Caspase8、Caspase9基因表达升高。结论：雨水藻提取物具有潜在的抗癌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.