Muhammad Sufyan Tahir, Ayesha Latif, Samina Bashir, Mohsin Shad, Muhammad Azmat Ullah Khan, Ambreen Gul, Naila Shahid, Tayyab Husnain, Abdul Qayyum Rao, Ahmed Ali Shahid
{"title":"Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton).","authors":"Muhammad Sufyan Tahir, Ayesha Latif, Samina Bashir, Mohsin Shad, Muhammad Azmat Ullah Khan, Ambreen Gul, Naila Shahid, Tayyab Husnain, Abdul Qayyum Rao, Ahmed Ali Shahid","doi":"10.1080/21645698.2021.1885288","DOIUrl":"https://doi.org/10.1080/21645698.2021.1885288","url":null,"abstract":"<p><p><i>Gossypium arboreum</i> (Desi Cotton) holds a special place in cotton industry because of its inherent ability to withstand drought, salinity, and remarkable resistance to sucking pests and cotton leaf curl virus. However, it suffers yield losses due to weeds and bollworm infestation. Genetic modification of <i>G. arboreum</i> variety FBD-1 was attempted in the current study to combat insect and weedicide resistance by incorporating <i>cry1Ac, cry2A</i> and <i>cp4-EPSPS</i> genes under control of 35S promoter in two different cassettes using kanamycin and GUS as markers through <i>Agrobacterium</i>-mediated shoot apex cut method of cotton transformation. The efficiency of transformation was found to be 1.57%. Amplification of 1700 bp for <i>cry1Ac</i>, 167 bp for <i>cry2A</i> and 111 bp for <i>cp4-EPSPS</i> confirmed the presence of transgenes in cotton plants. The maximum mRNA expression of <i>cry1Ac</i> and <i>cp4-EPSPS</i> was observed in transgenic cotton line L3 while minimum in transgenic cotton line L1. The maximum protein concentrations of Cry1Ac, Cry2A and Cp4-EPSPS of 3.534 µg g<sup>-1</sup>, 2.534 µg g<sup>-1</sup> and 3.58 µg-g<sup>-1</sup> respectively were observed for transgenic cotton line L3 as compared to control cotton line. On leaf-feed-based insect bioassay, almost 99% mortality was observed for <i>Helicoverpa armigera</i> on the transgenic cotton plant (L3). It completely survived the 1900 ml hectare<sup>-1</sup> glyphosate spray assay as compared to non-transgenic cotton plants. The necrotic spots appeared on the third day, leading to the complete death of control plants on the fifth day of assay. The successful multiple gene-stacking in <i>G. arboreum</i> FBD-1 variety could be further used for qualitative improvement of cotton fiber through plant breeding techniques.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"292-302"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1885288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25425843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integration of mRNA and miRNA profiling reveals the heterosis of three hybrid combinations of <i>Capsicum annuum</i> varieties.","authors":"Sha Yang, Zhuqing Zhang, Wenchao Chen, Xuefeng Li, Shudong Zhou, Chengliang Liang, Xin Li, Bozhi Yang, Xuexiao Zou, Feng Liu, Lijun Ou, Yanqing Ma","doi":"10.1080/21645698.2020.1852064","DOIUrl":"https://doi.org/10.1080/21645698.2020.1852064","url":null,"abstract":"<p><p><i>Capsicum annuum</i> is also known as chili which is one of the most important vegetable crops grown in the world. Breeding new varieties with heterosis could improve the quality of pepper, increase yield, growth potential, disease resistance, adaptability, and seed viability. To investigate the heterosis among three cross combinations of different parents, the mRNA-miRNA integrated analysis was performed. A total number of 22,659,009 to 36,423,818 clean data were generated from mRNA-seq with 81 libraries, and the unique mapped reads were from 35,495,567 (86.81%) to 46,466,622 (88.95%). The plant-hormone signal transduction pathway (40 genes) was detected with a higher DEG number. The <i>SAUR32L, GID1, PYR1, EIN2. ERF1, PR1, JAR1-like, IAA</i> from this pathway play a key role in plant development. From the miRNA-seq, the number of clean reads was ranging from 12,132,221 to 25,632,680. A total of 220 miRNAs were predicted in this study, and all of them were identified as novel miRNA. The top three candidate KEGG pathways of miRNA were ribosome signaling pathway (13 miRNAs), spliceosome pathway (13 miRNAs), and plant hormone signal transduction pathways (10 miRNAs). With the mRNA and miRNA integrated analysis, we found some key genes were regulated by some miRNAs. Among them, the scarecrow-like 6 protein can be up or down regulated by mir8, mir120, mir184, mir_214, mir125, and mir130. The function of Della protein was regulated by mir24, mir74, mir94, mir139, and mir190. This study contributes to understanding how heterosis regulates the traits, such as crop production, fruit weight, and fruit length.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"224-241"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1852064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38793169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reda E A Moghaieb, Dalia S Ahmed, Ahmed Gaber, Abdelhadi A Abdelhadi
{"title":"Overexpression of bacterial <i>katE</i> gene improves the resistance of modified tomato plant against <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i>.","authors":"Reda E A Moghaieb, Dalia S Ahmed, Ahmed Gaber, Abdelhadi A Abdelhadi","doi":"10.1080/21645698.2021.1903374","DOIUrl":"https://doi.org/10.1080/21645698.2021.1903374","url":null,"abstract":"<p><p>Tomato (<i>Solanum lycopersicum</i> L.) yield is severely affected by <i>Fusarium</i> fungal disease. To improve the resistance of tomato against Fusarium oxysporum f. sp. lycopersici (FOL), Escherichia coli katE gene was transformed into two tomato cultivars, namely Castle Rock and Super strain B, via <i>Agrobacterium tumefaciens</i>; the transformation efficiency was 5.6% and 3.5%, respectively. The integration of the <i>katE</i> gene into T<sub>0</sub>, T<sub>1</sub>, and T<sub>2</sub> transgenic tomato lines was confirmed using PCR. In addition, DNA dot blot technique confirmed the integration of the <i>katE</i> gene into T<sub>2</sub> transgenic tomato lines. The RT-PCR analysis confirmed that the <i>katE</i> gene could be expressed normally in the T<sub>2</sub> modified lines. Under artificial infection with FOL, the non-modified plants exhibited more severe fungal disease symptoms than those observed in <i>katE</i> overexpression (OE) lines. Our analysis showed that the levels of three defense enzymes, namely superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), were increased during transgenic T<sub>2</sub> generation pre-treated with FOL. The bioassay of modified lines revealed that an average of 52.56% of the modified Castle Rock cultivar and 50.28% of the modified Super Strain B cultivar showed resistance under <i>Fusarium</i> infection. These results clearly indicate that the modified tomato plants, in which the <i>katE</i> gene was overexpressed, became more resistant to the infection by FOL than the wild-type plants. Our study has proven that the overexpression of the <i>E. coli katE</i> gene in the OE lines could be utilized to develop and improve the resistance against fungal diseases in the modified crops.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"315-327"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1903374","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25544484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Resveratrol-enriched rice identical to original Dongjin rice variety with respect to major agronomic traits in different cultivation years and regions.","authors":"Vipada Kantayos, Woon-Chul Shin, Jin-Suk Kim, Seung-Ho Jeon, Eui-Shik Rha, So-Hyeon Baek","doi":"10.1080/21645698.2021.1979368","DOIUrl":"10.1080/21645698.2021.1979368","url":null,"abstract":"<p><p>Resveratrol is synthesized by the catalysis of resveratrol synthases (RS) in a limited number of higher plants. Resveratrol shows potential health-promoting properties, including as an antioxidant and in preventing cardiovascular diseases. Recently, resveratrol-enriched rice has been produced as a novel source of resveratrol. This study aimed to investigate the major agronomic characteristics of resveratrol-enriched rice, Iksan526 (I526) and compared them with those of a nontransgenic and commercial rice variety, Dongjin (DJ). Transgene (RS) integration was confirmed using Southern blot analysis, and homologous recombination was achieved after digestion with the SacI restriction enzyme. The phenotypic traits of I526 grown in Iksan were similar to those grown in Milyang but not similar to those grown in Suwon. In Suwon, I526 had slightly earlier heading dates [i.e., number of days from sowing to heading) and shorter culm lengths. When I526 was treated with 0.4% Basta in the seedling stage, no significant difference was observed among all the agronomic traits compared with nontreated I526; particularly, the culm length, panicle length, number of panicles per hill, 1,000 grain weight of brown rice, and brown rice yield of the Basta-treated rice were similar to those of the nontreated I526, regardless of their cultivation region. The resveratrol content of I526 grown in Suwon and Milyang was increased by 18% and 37%, respectively, than that of I526 grown in the Iksan area. Therefore, DJ and I526 are not significantly different in terms of major agronomic traits depending on variety/year and variety/cultivation region. The results indicated that I526 has the potential to become a commercialized variety in the near future.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"449-458"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39815135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Raza, Ali Razzaq, Sundas Saher Mehmood, Muhammad Azhar Hussain, Su Wei, Huang He, Qamar U Zaman, Zhang Xuekun, Mirza Hasanuzzaman
{"title":"Omics: The way forward to enhance abiotic stress tolerance in <i>Brassica napus</i> L.","authors":"Ali Raza, Ali Razzaq, Sundas Saher Mehmood, Muhammad Azhar Hussain, Su Wei, Huang He, Qamar U Zaman, Zhang Xuekun, Mirza Hasanuzzaman","doi":"10.1080/21645698.2020.1859898","DOIUrl":"https://doi.org/10.1080/21645698.2020.1859898","url":null,"abstract":"<p><p>Plant abiotic stresses negative affects growth and development, causing a massive reduction in global agricultural production. Rapeseed (<i>Brassica napus</i> L.) is a major oilseed crop because of its economic value and oilseed production. However, its productivity has been reduced by many environmental adversities. Therefore, it is a prime need to grow rapeseed cultivars, which can withstand numerous abiotic stresses. To understand the various molecular and cellular mechanisms underlying the abiotic stress tolerance and improvement in rapeseed, omics approaches have been extensively employed in recent years. This review summarized the recent advancement in genomics, transcriptomics, proteomics, metabolomics, and their imploration in abiotic stress regulation in rapeseed. Some persisting bottlenecks have been highlighted, demanding proper attention to fully explore the omics tools. Further, the potential prospects of the CRISPR/Cas9 system for genome editing to assist molecular breeding in developing abiotic stress-tolerant rapeseed genotypes have also been explained. In short, the combination of integrated omics, genome editing, and speed breeding can alter rapeseed production worldwide.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"251-281"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1859898","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38836026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fitness of Insect-resistant transgenic rice T1C-19 under four growing conditions combining land use and weed competition.","authors":"Jianmei Fu, Biao Liu, Laipan Liu, Zhixiang Fang","doi":"10.1080/21645698.2021.1914290","DOIUrl":"https://doi.org/10.1080/21645698.2021.1914290","url":null,"abstract":"<p><p>Transgene escape into natural ecosystems through seed spraying or transgene introgression may potentially cause environmental biosafety problems. In this study, we assessed the environmental risk of insect-resistant transgenic rice entering farmland margins or natural ecosystems adjacent to farmland. Transgenic <i>Cry1C*</i> rice (T1C-19) was used to study the effects of exogenous <i>Cry1C</i>* expression on vegetative and reproductive growth indices under different growing conditions using the following four combined treatments of land use and weeds: farmland and uncultivated land without weeds (F-NW and U-NW, respectively), and farmland and uncultivated land with weeds (F-W and U-W, respectively). The expression of Cry1C* protein under the U-NW, F-W, and U-W conditions was significantly lower than under the control condition, F-NW. Tiller number, biomass, filled grain number, filled grain weight, and other vegetative and reproductive indices were significantly lower in the rice line TIC-19 than in MH63 under F-NW and U-NW conditions, indicating a significant fitness cost. However, under F-W and U-W conditions, vegetative growth indices such as plant height, tiller number, and biomass, as well as reproductive growth indices such as filled grain number per plant, filled grain weight per plant, and seed setting rate in TIC-19 were similar to those in MH63, indicating a long-term coexistence. These results indicate a lower ecological risk of T1C-19 compared to MH63 under F-NW and U-NW, although their long-term coexistence may lead to potential ecological risks under F-W and U-W.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"328-341"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1914290","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38902503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brenda L Smith, Cindi S Zimmermann, Anne B Carlson, Carey A Mathesius, Pushkor Mukerji, James L McNaughton, Carl A Walker, Jason M Roper
{"title":"Evaluation of the safety and nutritional equivalency of maize grain with genetically modified event DP-Ø23211-2.","authors":"Brenda L Smith, Cindi S Zimmermann, Anne B Carlson, Carey A Mathesius, Pushkor Mukerji, James L McNaughton, Carl A Walker, Jason M Roper","doi":"10.1080/21645698.2021.1963614","DOIUrl":"10.1080/21645698.2021.1963614","url":null,"abstract":"<p><p>Feeding studies were conducted with rats and broiler chickens to assess the safety and nutrition of maize grain containing event DP-Ø23211-2 (DP23211), a newly developed trait-pyramid product for corn rootworm management. Diets containing 50% ground maize grain from DP23211, non-transgenic control, or non-transgenic reference hybrids (P0928, P0993, and P1105) were fed to Crl:CD®(SD) rats for 90 days. Ross 708 broilers were fed phase diets containing up to 67% maize grain from each source for 42 days. Body weight, gain, and feed conversion were determined for comparisons between animals fed DP23211 and control diets in each study. Additional measures included clinical and neurobehavioral evaluations, ophthalmology, clinical pathology, organ weights, and gross and microscopic pathology for rats, and carcass parts and select organ yields for broilers. Reference groups were included to determine if any observed significant differences between DP23211 and control groups were likely due to natural variation. No diet-related effects on mortality or evaluation measures were observed between animal fed diets produced with DP23211 maize grain and animal fed diets produced with control maize grain. These studies show that maize grain containing event DP-Ø23211-2 is as safe and nutritious as non-transgenic maize grains when fed in nutritionally adequate diets. The results are consistent with previously published studies, providing further demonstration of the absence of hazards from edible-fraction consumption of genetically modified plants.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"396-408"},"PeriodicalIF":4.5,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39366430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nidia Benítez Candia, Gabriela Ulke Mayans, Pilar Gómez Paniagua, Claudia Rezende Ribeiro, José Velázquez Franco, Daigo Kamada, Laura Mendoza de Arbo, Danilo Fernández Ríos
{"title":"Perception of genetically engineered crops in Paraguay.","authors":"Nidia Benítez Candia, Gabriela Ulke Mayans, Pilar Gómez Paniagua, Claudia Rezende Ribeiro, José Velázquez Franco, Daigo Kamada, Laura Mendoza de Arbo, Danilo Fernández Ríos","doi":"10.1080/21645698.2021.1969835","DOIUrl":"https://doi.org/10.1080/21645698.2021.1969835","url":null,"abstract":"<p><p>Paraguay is integrated into the world mainly through its agricultural activity. The population's perception of genetically engineered crops is relevant to design communication strategies that convey the advantages and limitations of the various technologies used in the country. We aimed to know the perception of the population of four Departments of the country where such crops are grown through a survey, which revealed a low level of knowledge about genetically engineered crops in general, and specifically about the effects of genetically engineered crops on production, nutrition, and the environment. Respondents expressed a willingness to receive information on genetically engineered crops, in particular from the <i>National Government and the Health Sector</i>.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"409-418"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e8/09/KGMC_12_1969835.PMC8475557.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39443021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guoping Li, Hongqiang Feng, Tingjie Ji, Jianrong Huang, Caihong Tian
{"title":"What type of Bt corn is suitable for a region with diverse lepidopteran pests: A laboratory evaluation.","authors":"Guoping Li, Hongqiang Feng, Tingjie Ji, Jianrong Huang, Caihong Tian","doi":"10.1080/21645698.2020.1831728","DOIUrl":"https://doi.org/10.1080/21645698.2020.1831728","url":null,"abstract":"<p><p>Transgenic crops that produce <i>Bacillus thuringiensis</i> (Bt) toxins are effective tools for controlling lepidopteran pests. However, the degree of susceptibility to Bt toxins differs among various pest species due to relatively narrow spectrum and high selectivity of such toxins. Bt corn hybrids for Chinese market were designed to target Asian corn borer <i>Ostrinia furnacalis</i> (Guenée), while their efficacy against other lepidopteran pests are not well defined, such as <i>Conogethes punctiferalis</i> (Guenée), <i>Helicoverpa armigera</i> (Hübner), <i>Agrotis ypsilon</i> (Rottemberg), and <i>Mythimna separata</i> (Walker), which are also important lepidopteran pests on corn in the Huang-Huai-Hai Summer Corn Region of China. To determine what type of Bt corn is suitable for this region, the efficacy of five Bt toxins, i.e., Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, and Vip3A, to these five lepidopteran species was evaluated in laboratory. Both <i>O. furnacalis</i> and <i>C. punctiferalis</i> showed similar high susceptibility to all five Bt toxins. <i>A. ypsilon</i> and <i>M. separate</i> were less sensitive to Cry1Ab and Cry1Ac than the other species. <i>H. armigera, A. ypsilon</i> and <i>M. separate</i> were less sensitive to Cry1F than <i>O. furnacalis</i> and <i>C. punctiferalis. H. armigera</i> was more sensitive to Cry2Ab than other tested species. All five species were equally sensitive to Vip3A, though their LC<sub>50</sub>s were all relatively higher. These findings suggest that the first generation Bt corn expressing single Cry1 toxin should not be the first choice because of the potential risk of control failure or less efficacy against <i>H. armigera, A. ypsilon</i> or <i>M. separate</i>. The second-generation Bt corn expressing Cry1 and Cry2 toxins, or the third generation Bt corn expressing Cry1, Cry2 and Vip3A toxins might produce better protection of corn in the Huang-Huai-Hai Summer Corn Region of China.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"115-124"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1831728","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38513569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sumran Ali, Muhammad Asim Nawaz, Muhammad Ghufran, Sumaira Nazar Hussain, Aljaifi Saddam Hussein Mohammed
{"title":"GM trust shaped by trust determinants with the impact of risk/benefit framework: the contingent role of food technology neophobia.","authors":"Sumran Ali, Muhammad Asim Nawaz, Muhammad Ghufran, Sumaira Nazar Hussain, Aljaifi Saddam Hussein Mohammed","doi":"10.1080/21645698.2020.1848230","DOIUrl":"10.1080/21645698.2020.1848230","url":null,"abstract":"<p><p>The present study is comparative in natures that focus on understanding the factors that influence the GM food trust level in the BRA framework and food technology neophobia in China and the USA. For this purpose, we collected 300 and 350 valid responses, respectively, through a structured questionnaire. By carefully evaluating the above relationships, we found that trust determinants such as institutional trust, technology trust, information revealed with GM food vary across both datasets. However, GM knowledge has a better association with GM food trust in both cases. Apart from this, the food technology neophobia slightly moderates the benefits-risk perception of consumers and GM trust. This study guides the policymakers to enhance GM knowledge, as GM food is scientifically proven safe for health and environment and can be a financial incentive for the farmers. Further, the study also provides direction for corporate managers to design effective marketing and communication strategies in two different countries by investigating GM food trust's primary motivators in both nations.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"170-191"},"PeriodicalIF":4.5,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38746350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}