Gm Crops & Food-Biotechnology in Agriculture and the Food Chain最新文献

{"title":"Modeling antecedent factors involved in behavioral intention towards technology application of genetically modified crops","authors":"Yahya Safi Sis, A. Rezaei, Hamid Karimi, P. Ataei","doi":"10.1080/21645698.2022.2057160","DOIUrl":"https://doi.org/10.1080/21645698.2022.2057160","url":null,"abstract":"ABSTRACT This research aimed to study behavioral intention toward genetically modified crop (GMC) technology. The statistical population was composed of all staff experts of Jihad-e Agriculture Organization of Iran (N = 837). The sample size was 310 agricultural experts taken by simple randomization. The data collection tool was a questionnaire. Data were analyzed by structural equations modeling. The results provided enough evidence to confirm the negative and significant effect of ethical concerns on behavioral intention toward GMC technology and the positive and significant effect of attitude toward technology and social impact on behavioral intention. According to the results, it is necessary to hold training courses inside and outside the country, adopt bottom-up management, use experienced and prospective managers, involve experts in planning and development of GMCs to a greater extent, and share personal experiences in training courses to change people’ attitude.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"13 1","pages":"50 - 64"},"PeriodicalIF":3.9,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49325783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel plant breeding techniques to advance nitrogen use efficiency in rice: A review.","authors":"Sajid Fiaz,&nbsp;Xiukang Wang,&nbsp;Sher Aslam Khan,&nbsp;Sunny Ahmar,&nbsp;Mehmood Ali Noor,&nbsp;Aamir Riaz,&nbsp;Kazim Ali,&nbsp;Farhat Abbas,&nbsp;Freddy Mora-Poblete,&nbsp;Carlos R Figueroa,&nbsp;Badr Alharthi","doi":"10.1080/21645698.2021.1921545","DOIUrl":"https://doi.org/10.1080/21645698.2021.1921545","url":null,"abstract":"<p><p>Recently, there has been a remarkable increase in rice production owing to genetic improvement and increase in application of synthetic fertilizers. For sustainable agriculture, there is dire need to maintain a balance between profitability and input cost. To meet the steady growing demands of the farming community, researchers are utilizing all available resources to identify nutrient use efficient germplasm, but with very little success. Therefore, it is essential to understand the underlying genetic mechanism controlling nutrients efficiency, with the nitrogen use efficiency (NUE) being the most important trait. Information regarding genetic factors controlling nitrogen (N) transporters, assimilators, and remobilizers can help to identify candidate germplasms via high-throughput technologies. Large-scale field trials have provided morphological, physiological, and biochemical trait data for the detection of genomic regions controlling NUE. The functional aspects of these attributes are time-consuming, costly, labor-intensive, and less accurate. Therefore, the application of novel plant breeding techniques (NPBTs) with context to genome engineering has opened new avenues of research for crop improvement programs. Most recently, genome editing technologies (GETs) have undergone enormous development with various versions from Cas9, Cpf1, base, and prime editing. These GETs have been vigorously adapted in plant sciences for novel trait development to insure food quantity and quality. Base editing has been successfully applied to improve NUE in rice, demonstrating the potential of GETs to develop germplasms with improved resource use efficiency. NPBTs continue to face regulatory setbacks in some countries due to genome editing being categorized in the same category as genetically modified (GM) crops. Therefore, it is essential to involve all stakeholders in a detailed discussion on NPBTs and to formulate uniform policies tackling biosafety, social, ethical, and environmental concerns. In the current review, we have discussed the genetic mechanism of NUE and NPBTs for crop improvement programs with proof of concepts, transgenic and GET application for the development of NUE germplasms, and regulatory aspects of genome edited crops with future directions considering NUE.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"627-646"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1921545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39031227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering broad-spectrum resistance to cotton leaf curl disease by CRISPR-Cas9 based multiplex editing in plants.","authors":"Muhammad Salman Mubarik,&nbsp;Xiukang Wang,&nbsp;Sultan Habibullah Khan,&nbsp;Aftab Ahmad,&nbsp;Zulqurnain Khan,&nbsp;Muhammad Waqas Amjid,&nbsp;Muhammad Khuram Razzaq,&nbsp;Zulfiqar Ali,&nbsp;Muhammad Tehseen Azhar","doi":"10.1080/21645698.2021.1938488","DOIUrl":"https://doi.org/10.1080/21645698.2021.1938488","url":null,"abstract":"<p><p>Advances in genome editing technologies have tremendous potential to address the limitations of classical resistance breeding. CRISPR-Cas9 based gene editing has been applied successfully in plants to tolerate virus infections. In this study, we successfully tested CRISPR-Cas9 system to counteract cotton leaf curl disease (CLCuD) caused by whitefly transmitted cotton leaf curl viruses (CLCuVs). We also analyzed the ability of CLCuV to escape the Cas9 endonuclease activity. Targeting overlapping genes of most prevalent CLCuVs with three gRNAs resulted in virus interference, as validated by low virus titer. Furthermore, multiplex CRISPR-Cas9 construct simultaneously targeting six genes of CLCuV, was found more effective to interfere with virus proliferation compared to targeting single region individually. Additionally, transgenic <i>N. benthamiana</i> plants expressing multiple gRNAs simultaneously showed enhanced tolerance against CLCuV infection when compared to wild-type plants. T7 Endonuclease-I (T7EI) assay, showing indels in the CLCuV genome, confirmed the occurrence of double strand breaks (DSBs) in DNA at target sequence induced by Cas9 endonuclease. We observed that targeting CLCuV genome at multiple sites simultaneously resulted in better interference, also with inefficient recovery of altered virus molecules. Next, we tested multiplex construct in cotton to interfere CLCuV infection. We found significant decrease in virus accumulation in cotton leaves co-infiltrated with multiplex cassette and virus compared to cotton leaves infiltrated with virus only. The results demonstrate future use of CRISPR-Cas9 system for engineering virus resistance in crops. Moreover, our results also advocate that resistance to mixed virus infections can be engineered using multiplex genome editing.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"647-658"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1938488","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39091673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene editing isn't just about food: comments from U.S. focus groups.","authors":"Brandon R McFadden,&nbsp;Joy N Rumble,&nbsp;Kathryn A Stofer,&nbsp;Kevin M Folta,&nbsp;Savanna Turner,&nbsp;Adam Pollack","doi":"10.1080/21645698.2021.1919485","DOIUrl":"https://doi.org/10.1080/21645698.2021.1919485","url":null,"abstract":"<p><p>In the United States, adult public perception of genetic modification has been well documented in the domain of agriculture and food; however, recent international news on gene editing in medical applications may present new challenges for science communicators who seek to proactively share benefits of emerging gene editing technology. While research traditionally considers perceptions of agricultural and medical applications separately, gene editing may bridge the gap between the two domains. We find that when asked about thoughts regarding gene editing, adult focus groups discussed medical applications more frequently and extensively than agricultural applications. Although, when examining the length of discussion about specific topics, designer babies, cures for disease, and food were discussed at similar lengths. Understanding audiences' current perceptions of the technology is the first step in shaping strategic communication efforts to inform public opinion. A proper understanding of the benefits and risks of new technology is central to its application.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"616-626"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1919485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39000752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing techniques in plants: a comprehensive review and future prospects toward zero hunger.","authors":"Naglaa A Abdallah,&nbsp;Aladdin Hamwieh,&nbsp;Khaled Radwan,&nbsp;Nourhan Fouad,&nbsp;Channapatna Prakash","doi":"10.1080/21645698.2021.2021724","DOIUrl":"10.1080/21645698.2021.2021724","url":null,"abstract":"<p><p>Promoting sustainable agriculture and improving nutrition are the main united nation sustainable development goals by 2030. New technologies are required to achieve zero hunger, and genome editing technology is the most promising one. In the last decade, genome editing (GE) using the CRISPR/Cas system has attracted researchers as a safer and easy tool for genome editing in several living organisms. GE has revolutionized the field of agriculture by improving biotic and abiotic stresses and yield improvement. GE technologies were developed fast lately to avoid the obstacles that face GM crops. GE technology, depending on site directed nuclease (SDN), is divided into three categories according to the modification methods. Developing transgenic-free edited plants without introducing foreign DNA meet the acceptance and regulatory ratification of several countries. There are several ongoing efforts from different countries that are rapidly expanding to adopt the current technological innovations. This review summarizes the different GE technologies and their application as a way to help in ending hunger.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"601-615"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39900643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop Biotechnology and Product Stewardship.","authors":"Ruth Mbabazi,&nbsp;Muffy Koch,&nbsp;Karim Maredia,&nbsp;Joseph Guenthner","doi":"10.1080/21645698.2020.1822133","DOIUrl":"https://doi.org/10.1080/21645698.2020.1822133","url":null,"abstract":"<p><p>Agricultural biotechnology is enhancing agricultural productivity, food security, and livelihoods globally. Some developing countries have established functional biosafety regulatory systems and have commercialized genetically modified (GM) crops. Release of GM crops requires enhanced capacity for regulatory compliance and product stewardship to help ensure sustainable use of biotechnology products. We conducted a survey of 66 stakeholders, mostly from Africa and Asia, in two-week international agricultural biotechnology short courses. Respondents showed knowledge of biotechnology benefits and expressed potential barriers to commercialization. They identified 16 crops in the \"pipeline for commercialization.\" Stakeholders also shared ideas about how to build capacity for product stewardship. Product stewardship is a concept which requires each person in the product life cycle - innovators, scientists, and technology users, to share responsibility. This paper focuses on adoption of product stewardship for post-release management of GM crops which encompasses trait performance, resistance management, integrated pest management (IPM), good agricultural practices, high-quality seeds and planting material, intellectual property management, labeling, identity preservation, consumer acceptance, and effective marketing.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"106-114"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1822133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38508633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of consumers' knowledge on their responses to genetically modified foods.","authors":"Hyesun Hwang,&nbsp;Su-Jung Nam","doi":"10.1080/21645698.2020.1840911","DOIUrl":"https://doi.org/10.1080/21645698.2020.1840911","url":null,"abstract":"<p><p>This study examined the influence of consumers' knowledge on their perceptions and purchase intentions toward genetically modified foods, and the implications of these consumer responses for sustainable development in the food industry. This study distinguished between objective and subjective knowledge and identified how an imbalance between the two knowledge types influenced consumers' attitudes and purchase intentions toward genetically modified foods. Results of a multinomial regression analysis showed that consumers with higher levels of education, income, and food involvement and more exposure to negative information about genetically modified foods tended to overestimate their actual knowledge level. The overestimation group showed a higher risk perception, lower benefit perception, and lower intention to purchase genetically modified foods than other participants. Consumers with less education and higher income were more likely to underestimate their knowledge.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"146-157"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1840911","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38565044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coexistence of genetically modified seed production and organic farming in Chile.","authors":"M A Sánchez,&nbsp;H Campos","doi":"10.1080/21645698.2021.2001242","DOIUrl":"https://doi.org/10.1080/21645698.2021.2001242","url":null,"abstract":"<p><p>The seed industry in Chile has thrived since the implementation of a stringent, voluntarily self-imposed coexistence strategy between genetically modified organisms (GMOs) and non-GMO seed activities. GMO varieties of maize, soybean, and canola represent the vast majority of biotech seeds produced in Chile. Chile's exports of genetically modified (GM) seeds and organically grown food products (which excludes GM seeds and materials) continue to expand. Organic Chilean farmers predominantly produce and export fruits such as blueberries, wine grapes, and apples. Under normal agricultural conditions, the inadvertent presence of GMOs in non-GMO or organic crops cannot be ruled out. Producers of organic foods are required to implement stringent measures to minimize contact with any non-organic crop, regardless of whether these crops are GM. Only very small amounts of organic maize, soybean, and canola - if any - have been produced in Chile in recent years. Given the characteristics and nature of Chile's agriculture, the direct impact of the GM seed industry on organic farming in Chile is likely to be negligible. The Chilean experience with coexistence between GM seed and organic industries may inform other countries interested in providing its farmers with alternative agricultural production systems.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"509-519"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypothesis-based food, feed, and environmental safety assessment of GM crops: A case study using maize event DP-202216-6.","authors":"Jennifer A Anderson,&nbsp;Rod A Herman,&nbsp;Anne Carlson,&nbsp;Carey Mathesius,&nbsp;Carl Maxwell,&nbsp;Henry Mirsky,&nbsp;Jason Roper,&nbsp;Brenda Smith,&nbsp;Carl Walker,&nbsp;Jingrui Wu","doi":"10.1080/21645698.2020.1869492","DOIUrl":"https://doi.org/10.1080/21645698.2020.1869492","url":null,"abstract":"<p><p>Event DP-2Ø2216-6 (referred to as DP202216 maize) was genetically modified to increase and extend the expression of the introduced <i>zmm28</i> gene relative to endogenous <i>zmm28</i> gene expression, resulting in plants with enhanced grain yield potential. The <i>zmm28</i> gene expresses the ZMM28 protein, a MADS-box transcription factor. The safety assessment of DP202216 maize included an assessment of the potential hazard of the ZMM28 protein, as well as an assessment of potential unintended effects of the genetic insertion on agronomics, composition, and nutrition. The history of safe use (HOSU) of the ZMM28 protein was evaluated and a bioinformatics approach was used to compare the deduced amino acid sequence of the ZMM28 protein to databases of known allergens and toxins. Based on HOSU and the bioinformatics assessment, the ZMM28 protein was determined to be unlikely to be either allergenic or toxic to humans. The composition of DP202216 maize forage and grain was comparable to non-modified forage and grain, with no unintended effects on nutrition or food and feed safety. Additionally, feeding studies with broiler chickens and rats demonstrated a low likelihood of unintentional alterations in nutrition and low potential for adverse effects. Furthermore, the agronomics observed for DP202216 maize and non-modified maize were comparable, indicating that the likelihood of increased weediness or invasiveness of DP202216 maize in the environment is low. This comprehensive review serves as a reference for regulatory agencies and decision-makers in countries where authorization of DP202216 maize will be pursued, and for others interested in food, feed, and environmental safety.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"282-291"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1869492","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38842069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative compositional analysis of cassava brown streak disease resistant 4046 cassava and its non-transgenic parental cultivar.","authors":"H Wagaba,&nbsp;P Kuria,&nbsp;P Wangari,&nbsp;J Aleu,&nbsp;H Obiero,&nbsp;G Beyene,&nbsp;T Alicai,&nbsp;A Bua,&nbsp;W Esuma,&nbsp;E Nuwamanya,&nbsp;S Gichuki,&nbsp;D Miano,&nbsp;P Raymond,&nbsp;A Kiggundu,&nbsp;N Taylor,&nbsp;B M Zawedde,&nbsp;C Taracha,&nbsp;D J MacKenzie","doi":"10.1080/21645698.2020.1836924","DOIUrl":"https://doi.org/10.1080/21645698.2020.1836924","url":null,"abstract":"<p><p>Compositional analysis is an important component of an integrated comparative approach to assessing the food and feed safety of new crops developed using biotechnology. As part of the safety assessment of cassava brown streak disease resistant 4046 cassava, a comprehensive assessment of proximates, minerals, amino acids, fatty acids, vitamins, anti-nutrients, and secondary metabolites was performed on leaf and storage root samples of 4046 cassava and its non-transgenic parental control, TME 204, collected from confined field trials in Kenya and Uganda over two successive cropping cycles. Among the 100 compositional components that were assessed in samples of 4046 and control TME 204 cassava roots (47 components) and leaves (53 components), there were no nutritionally relevant differences noted. Although there were statistically significant differences between the transgenic and control samples for some parameters, in most cases the magnitudes of these differences were small ( <math><mo><</mo></math> 20%), and in every case where comparative literature data were available, the mean values for 4046 and control cassava samples were within the range of normal variation reported for the compositional component in question. Overall, no consistent patterns emerged to suggest that biologically meaningful adverse changes in the composition or nutritive value of the leaves or storage roots occurred as an unintended or unexpected consequence of the genetic modification resulting in 4046 cassava. The data presented here provide convincing evidence of the safety of 4046 cassava with respect to its biochemical composition for food and feed, and it could be considered as safe as its non-transgenic control.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"158-169"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1836924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}