Gm Crops & Food-Biotechnology in Agriculture and the Food Chain最新文献

{"title":"Golden opportunities? How marketing expectations drive purchase intentions of golden rice in Bangladesh and the Philippines.","authors":"Dilshad Zahan Ethen, Maimuna Begum, Berre Deltomme, Md Rasheduzzaman, Josefina F Ballesteros, Riza Abilgos-Ramos, Mohammad Jahangir Alam, Alice Onek Atimango, Hans De Steur","doi":"10.1080/21645698.2024.2418161","DOIUrl":"https://doi.org/10.1080/21645698.2024.2418161","url":null,"abstract":"<p><p>Golden Rice (GR), genetically modified (GM) rice enriched with provitamin A, holds promise to address micronutrient deficiencies in developing countries. However, its success hinges on market acceptance. This study investigates how the marketing aspects of GR influence consumers' purchase intentions in Bangladesh and the Philippines. The Expectation Confirmation Theory (ECT) is employed to analyze the role of expectations regarding the marketing mix components (i.e. product, price, place, promotion), risk perceptions, performance expectations, and expected satisfaction on consumers' purchase intentions. Data from online surveys in Bangladesh (<i>n</i> = 391) and the Philippines (<i>n</i> = 354), collected using convenience sampling, were analyzed using structural equation modeling. Findings reveal that positive expectations toward the marketing mix, performance, and satisfaction increase consumers' purchase intention of GR, whereas risk perceptions have a negative influence. Additionally, it was found that expectations toward all four marketing mix components significantly affect purchase intention in Bangladesh. However, only product and promotion have a notable influence in the Philippines. These results emphasize the importance of effectively addressing consumers' marketing expectations to help ensure a successful implementation. This study is novel as it delves into consumers' purchase intentions for a GM biofortified crop and their expectations for different aspects of its future marketing (i.e. product, price, place, promotion), performance, and satisfaction. If GR is commercialized, future research should validate these expectations based on actual consumer experiences. Additionally, longitudinal studies could track changes in consumer expectations over time, identifying consistently valued marketing elements and offering a valuable technique for product development before launch.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"316-335"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?","authors":"Sophia Edelmann, Christian Savini, Dominik Moor, Jörn Lämke, Kathrin Lieske, Marco Mazzara, Hendrik Emons, Joachim Mankertz, Christopher Weidner","doi":"10.1080/21645698.2024.2423441","DOIUrl":"10.1080/21645698.2024.2423441","url":null,"abstract":"<p><p>Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"352-360"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk assessment of homologous variants of biotech trait proteins using a bridging approach.","authors":"Cunxi Wang, Jennifer Calcaterra, Beverly Anderson, Timothy Rydel, Rong Wang, Lieselot Bertho, Scott A Saracco, Kimberly Hodge-Bell, Luis Burzio, Tommi White, Bin Li","doi":"10.1080/21645698.2024.2420412","DOIUrl":"10.1080/21645698.2024.2420412","url":null,"abstract":"<p><p>A transgenic protein is frequently expressed as different homologous variants in genetically modified crops due to differential processing of targeting peptides or optimization of activity and specificity. The aim of this study was to develop a science-based approach for risk assessment of homologous protein variants using dicamba mono-oxygenase (DMO) as a case study. In this study, DMO expressed in the next-generation dicamba-tolerant maize, sugar beet and soybean crops exhibited up to 27 amino acid sequence differences in the N-terminus. Structure modeling using AlphaFold, ESMFold and OpenFold demonstrates that these small N-terminal extensions lack an ordered secondary structure and do not disrupt the DMO functional structure. Three DMO variants were demonstrated to have equivalent immunoreactivity and functional activity ranging from 214 to 331 nmol/min/mg. Repeated toxicity studies using each DMO variant found no test substance-related adverse effects. These results support that homologous protein variants, which have demonstrated physicochemical and functional equivalence, can leverage existing safety data from one variant without requiring additional <i>de novo</i> safety assessments.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"336-351"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene editing achieves consistently higher favorability in social and traditional media than GMOs.","authors":"Mark Lynas, Selene Adams, Karen Stockert","doi":"10.1080/21645698.2023.2226889","DOIUrl":"10.1080/21645698.2023.2226889","url":null,"abstract":"<p><p>While GMOs have been the subject of negative discourse over a long time period, it is possible that newer breeding technologies like gene editing are viewed more favorably. We present data for a 5-year period between January 2018 and December 2022, showing that in content specific to agricultural biotechnology, gene editing achieves consistently higher favorability ratings than GMOs in both social and traditional English-language media. Our sentiment analysis shows that favorability is especially positive in social media, with close to 100% favorability achieved in numerous monthly values throughout our 5 years of analysis. We believe that the scientific community can therefore be cautiously optimistic based on current trends that gene editing will be accepted by the public and be able to achieve its promise of making a substantial contribution to future food security and environmental sustainability worldwide. However, there are some recent indications of more sustained downward trends, which may be a cause for concern.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-8"},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10042924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate.","authors":"Saeed Rauf, Seerat Fatima, Rodomiro Ortiz","doi":"10.1080/21645698.2023.2243041","DOIUrl":"10.1080/21645698.2023.2243041","url":null,"abstract":"<p><p>Mutation breeding based on various chemical and physical mutagens induces and disrupts non-target loci. Hence, large populations were required for visual screening, but desired plants were rare and it was a further laborious task to identify desirable mutants. Generated mutant had high defect due to non-targeted mutation, with poor agronomic performance. Mutation techniques were augmented by targeted induced local lesions in genome (TILLING) facilitating the selection of desirable germplasm. On the other hand, gene editing through CRISPR/Cas9 allows knocking down genes for site-directed mutation. This handy technique has been exploited for the modification of fatty acid profile. High oleic acid genetic stocks were obtained in a broad range of crops. Moreover, genes involved in the accumulation of undesirable seed components such as starch, polysaccharide, and flavors were knocked down to enhance seed quality, which helps to improve oil contents and reduces the anti-nutritional component.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-12"},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10326468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically modified DP915635 maize is agronomically and compositionally comparable to non-genetically modified maize.","authors":"Jennifer A Anderson, James Mickelson, Brandon J Fast, Nathan Smith, Robert C Pauli, Carl Walker","doi":"10.1080/21645698.2023.2208997","DOIUrl":"10.1080/21645698.2023.2208997","url":null,"abstract":"<p><p>DP915635 maize was genetically modified (GM) to express the IPD079Ea protein for corn rootworm (Diabrotica spp.) control. DP915635 maize also expresses the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide and the phosphomannose isomerase (PMI) protein that was used as a selectable marker. A field study was conducted at ten sites in the United States and Canada during the 2019 growing season. Of the 11 agronomic endpoints that were evaluated, two of them (early stand count and days to flowering) were statistically significant compared with the control maize based on unadjusted p-values; however, these differences were not significant after FDR-adjustment of p-values. Composition analytes from DP915635 maize grain and forage (proximates, fiber, minerals, amino acids, fatty acids, vitamins, anti-nutrients, and secondary metabolites) were compared to non-GM near-isoline control maize (control maize) and non-GM commercial maize (reference maize). Statistically significant differences were observed for 7 of the 79 compositional analytes (16:1 palmitoleic acid, 18:0 stearic acid, 18:1 oleic acid, 18:2 linoleic acid, 24:0 lignoceric acid, methionine, and α-tocopherol); however, these differences were not significant after FDR-adjustment. Additionally, all of the values for composition analytes fell within the range of natural variation established from the in-study reference range, literature range, and/or tolerance interval. These results demonstrate that DP915635 is agronomically and compositionally comparable to non-GM maize represented by non-GM near-isoline control maize and non-GM commercial maize.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"14 1","pages":"1-8"},"PeriodicalIF":4.5,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deconstruction of science hegemony: discursive strategies of Chinese science communication on genetically modified foods.","authors":"Jinrong Lin","doi":"10.1080/21645698.2023.2247133","DOIUrl":"10.1080/21645698.2023.2247133","url":null,"abstract":"<p><p>This study analyzes Chinese online science communication and discussion about genetically modified foods (GMFs). Based on data collected from one of the largest Chinese GMFs science popularization website, it utilizes Wodak's discourse analysis to investigate how Chinese genetically modified (GM) scientific discourse is characterized by a range of discursive strategies that construct specific identity of Chinese GM scientists and explore science hegemony underlying Chinese GMFs debates. Findings show that discourse features of Chinese GM scientific discourse is objective as well as emotional, particularly conveying a strong sense of superiority realized by trope, argumentative strategies and intertextuality. The unequal power relationship between scientists and the public as well as Chinese intellectuals' knowledge hegemony could partly explain why those discursive strategies are employed in science communication practices of GMFs debates in Chinese context.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-9"},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Processes for regulating genetically modified and gene edited plants.","authors":"John R Caradus","doi":"10.1080/21645698.2023.2252947","DOIUrl":"10.1080/21645698.2023.2252947","url":null,"abstract":"<p><p>Innovation in agriculture has been essential in improving productivity of crops and forages to support a growing population, improving living standards while contributing toward maintaining environment integrity, human health, and wellbeing through provision of more nutritious, varied, and abundant food sources. A crucial part of that innovation has involved a range of techniques for both expanding and exploiting the genetic potential of plants. However, some techniques used for generating new variation for plant breeders to exploit are deemed higher risk than others despite end products of both processes at times being for all intents and purposes identical for the benefits they provide. As a result, public concerns often triggered by poor communication from innovators, resulting in mistrust and suspicion has, in turn, caused the development of a range of regulatory systems. The logic and motivations for modes of regulation used are reviewed and how the benefits from use of these technologies can be delivered more efficiently and effectively is discussed.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-41"},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10192871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA-free genome editing for <i>ZmPLA1</i> gene via targeting immature embryos in tropical maize.","authors":"Sagar Krushnaji Rangari, Manjot Kaur Sudha, Harjot Kaur, Nidhi Uppal, Gagandeep Singh, Yogesh Vikal, Priti Sharma","doi":"10.1080/21645698.2023.2197303","DOIUrl":"10.1080/21645698.2023.2197303","url":null,"abstract":"<p><p>Doubled haploid (DH) production accelerates the development of homozygous lines in a single generation. In maize, haploids are widely produced by the use of haploid inducer Stock 6, earlier reported in 1959. Three independent studies reported haploid induction in maize which is triggered due to a 4 bp frame-shift mutation in <i>matrilineal</i> (<i>ZmPLA1</i>) gene. The present study was focused on the generation of mutants for <i>ZmPLA1</i> gene in maize inbred line LM13 through site-directed mutagenesis via CRISPR/Cas9-mediated ribonucleoprotein (RNP) complex method to increase the haploid induction rate. Three single guide RNAs (sgRNAs) for the <i>ZmPLA1</i> gene locus were used for transforming the 14 days old immature embryos via bombardment. 373 regenerated plants were subjected to mutation detection followed by Sanger's sequencing. Out of three putative mutants identified, one mutant depicted one base pair substitution and one base pair deletion at the target site.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-7"},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10761150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9254178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-gene editing approaches in plant breeding.","authors":"Himanshu Saini, Rajneesh Thakur, Rubina Gill, Kalpana Tyagi, Manika Goswami","doi":"10.1080/21645698.2023.2256930","DOIUrl":"10.1080/21645698.2023.2256930","url":null,"abstract":"<p><p>CRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and <i>in vitro</i> transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"14 1","pages":"1-17"},"PeriodicalIF":4.5,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41177475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}