Gm Crops & Food-Biotechnology in Agriculture and the Food Chain最新文献

{"title":"<i>ZmNF-YB10</i>, a maize NF-Y transcription factor, positively regulates drought and salt stress response in <i>Arabidopsis thaliana</i>.","authors":"Yimeng Wang, Peng Jiao, Chenyang Wu, Chunlai Wang, Ke Shi, Xiaoqi Gao, Shuyan Guan, Yiyong Ma","doi":"10.1080/21645698.2024.2438421","DOIUrl":"https://doi.org/10.1080/21645698.2024.2438421","url":null,"abstract":"<p><p>Maize (<i>Zea mays</i> L.) is a major food and feed crop and an important raw material for energy, chemicals, and livestock. The NF-Y family of transcription factors in maize plays a crucial role in the regulation of plant development and response to environmental stress. In this study, we successfully cloned and characterized the maize NF-Y transcription factor gene <i>ZmNF-YB10</i>. We used bioinformatics, quantitative fluorescence PCR, and other techniques to analyze the basic properties of the gene, its tissue expression specificity, and its role in response to drought, salt, and other stresses. The results indicated that the gene was 1209 base pairs (bp) in length, with a coding sequence (CDS) region of 618 bp, encoding a polypeptide composed of 205 amino acid residues. This polypeptide has a theoretical isoelectric point of 5.85 and features a conserved structural domain unique to the NF-Y family. Quantitative fluorescence PCR results demonstrated that the <i>ZmNF-YB10</i> gene was differentially upregulated under drought and salt stress treatments but exhibited a negatively regulated expression pattern under alkali and cold stress treatments. Transgenic <i>Arabidopsis thaliana</i> subjected to drought and salt stress in soil showed greener leaves than wild-type <i>A. thaliana</i>. In addition, the overexpression lines showed reduced levels of hydrogen peroxide (H₂O₂), superoxide (O^2-), and malondialdehyde (MDA) and increased activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD). Western blot analysis revealed a distinct band at 21.8 kDa. Salt and drought tolerance analyses conducted in <i>E. coli</i> BL21 indicated a positive regulation. In yeast cells, <i>ZmNF-YB10</i> exhibited a biological function that enhances salt and drought tolerance. Protein interactions were observed among the <i>ZmNF-YB10</i>, <i>ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes. It is hypothesized that the <i>ZmNF-YB10, ZmNF-YC2</i>, and <i>ZmNF-YC4</i> genes may play a role in the response to abiotic stresses, such as drought and salt tolerance, in maize.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"28-45"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat.","authors":"Naglaa A Abdallah, Hany Elsharawy, Hamiss A Abulela, Roger Thilmony, Abdelhadi A Abdelhadi, Nagwa I Elarabi","doi":"10.1080/21645698.2022.2120313","DOIUrl":"10.1080/21645698.2022.2120313","url":null,"abstract":"<p><p>Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3'(2'), 5'-bisphosphate nucleotidase (<i>TaSal1</i>) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active <i>TaSal1</i> homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat <i>TaSal1</i> genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable <i>TaSal1</i> mutations in the M₁ progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and bended stems, but there were no significant changes in the internode length and width, leaf morphology, and stem shape. Anatomical and scanning electron microscope studies of the young leaves of mutated <i>TaSal1</i> lines showed closed stomata, increased stomata width and increase in the size of the bulliform cells. <i>Sal1</i> mutant seedlings germinated and grew better on media containing polyethylene glycol than wildtype seedlings. Our results indicate that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing is efficient tool for mutating more multiple TaSal1 loci in hexaploid wheat.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-17"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33490173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and developmental strategies to hasten the improvement of orphan crops.","authors":"Ufuoma Akpojotor, Olubusayo Oluwole, Olaniyi Oyatomi, Rajneesh Paliwal, Michael Abberton","doi":"10.1080/21645698.2024.2423987","DOIUrl":"https://doi.org/10.1080/21645698.2024.2423987","url":null,"abstract":"<p><p>To feed the world's expanding population, crop breeders need to increase agricultural productivity and expand major crops base. Orphan crops are indigenously important crops with great potential because they are climate resilient, highly nutritious, contain nutraceutical compounds, and can improve the livelihood of smallholder farmers and consumers, but they have received little or no scientific attention. This review article examines several research and developmental strategies for hastening the improvement of these crops so that they can effectively play their role in securing food and nutrition. The integration of both research and developmental approaches will open up modern opportunities for crop improvement. We summarized ways in which advanced tools in phenotyping and genotyping, using high-throughput processes, can be used to accelerate their improvement. Finally, we suggest roles the genebanks can play in improving orphan crops, as the utilization of plant genetic resources is important for the genetic improvement of a crop.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"46-71"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agricultural chemical use and the rural-urban divide in Canada.","authors":"Stuart J Smyth, Sylvain Charlebois","doi":"10.1080/21645698.2024.2318876","DOIUrl":"10.1080/21645698.2024.2318876","url":null,"abstract":"<p><p>Innovation is of fundamental importance for improving food production, as well as sustainability food production. Since 1960, food production has benefited from innovations in plant breeding technologies, fertilizer, chemicals and equipment. These innovations have dramatically increased food production, while the amount of land used has minimally increased. However, future food production increases are jeopardized from widening knowledge gaps between rural food producers and large urban food consuming populations. Over time, that gap has fueled disinformation. The development of disinformation business models contributes to urban consumers receiving inaccurate information about the importance of inputs essential to food production, resulting in political pressures being applied that are targeted at reductions in the use of many food production inputs. The use of chemicals are a frequent target of disinformation campaigns. This article examines how the lack of government clarity about the safe use of chemicals contributes to a lack of public information.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"32-39"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the GMO narrative through labeling: strategies, products, and politics.","authors":"Camille D Ryan, Elizabeth Henggeler, Samantha Gilbert, Andrew J Schaul, John T Swarthout","doi":"10.1080/21645698.2024.2318027","DOIUrl":"10.1080/21645698.2024.2318027","url":null,"abstract":"<p><p>Labels are influential signals in the marketplace intended to inform and to eliminate buyer confusion. Despite this, food labels continue to be the subject of debate. None more so than non-GMO (genetically modified organisms) labels. This manuscript provides a timeline of the evolution of GMO labels beginning with the early history of the anti-GMO movement to the current National Bioengineered Food Disclosure Standard in the United States. Using media and market intelligence data collected through Buzzsumo™ and Mintel™, public discourse of GMOs is analyzed in relation to sociopolitical events and the number of new food products with anti-GMO labels, respectively. Policy document and publication data is collected with Overton™ to illustrate the policy landscape for the GMO topic and how it has changed over time. Analysis of the collective data illustrates that while social media and policy engagement around the topic of GMOs has diminished over time, the number of new products with a GMO-free designation continues to grow. While discourse peaked at one point, and has since declined, our results suggest that the legacy of an anti-GMO narrative remains firmly embedded in the social psyche, evidenced by the continuing rise of products with GMO-free designation. Campaigns for GMO food labels to satisfy consumers' right to know were successful and the perceived need for this information now appears to be self-sustaining.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"51-66"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>ShF5H1</i> overexpression increases syringyl lignin and improves saccharification in sugarcane leaves.","authors":"Juan Pablo Portilla Llerena, Eduardo Kiyota, Fernanda Raquel Camilo Dos Santos, Julio C Garcia, Rodrigo Faleiro de Lima, Juliana Lischka Sampaio Mayer, Michael Dos Santos Brito, Paulo Mazzafera, Silvana Creste, Paula Macedo Nobile","doi":"10.1080/21645698.2024.2325181","DOIUrl":"10.1080/21645698.2024.2325181","url":null,"abstract":"<p><p>The agricultural sugarcane residues, bagasse and straws, can be used for second-generation ethanol (2GE) production by the cellulose conversion into glucose (saccharification). However, the lignin content negatively impacts the saccharification process. This polymer is mainly composed of guaiacyl (G), hydroxyphenyl (H), and syringyl (S) units, the latter formed in the ferulate 5-hydroxylase (F5H) branch of the lignin biosynthesis pathway. We have generated transgenic lines overexpressing <i>ShF5H1</i> under the control of the <i>C4H</i> (cinnamate 4-hydroxylase) rice promoter, which led to a significant increase of up to 160% in the S/G ratio and 63% in the saccharification efficiency in leaves. Nevertheless, the content of lignin was unchanged in this organ. In culms, neither the S/G ratio nor sucrose accumulation was altered, suggesting that <i>ShF5H1</i> overexpression would not affect first-generation ethanol production. Interestingly, the bagasse showed a significantly higher fiber content. Our results indicate that the tissue-specific manipulation of the biosynthetic branch leading to S unit formation is industrially advantageous and has established a foundation for further studies aiming at refining lignin modifications. Thus, the <i>ShF5H1</i> overexpression in sugarcane emerges as an efficient strategy to improve 2GE production from straw.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"67-84"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gender integration of agricultural innovation: implications for the genetically modified crop product development pipeline.","authors":"Elizabeth Katz","doi":"10.1080/21645698.2024.2431203","DOIUrl":"10.1080/21645698.2024.2431203","url":null,"abstract":"<p><p>We provide guidance on how to incorporate best practices around gender integration in the development of genetically improved crops by adapting a gender integration framework for conventional crop breeding to the GM product development pipeline, which places greater emphasis on the discovery and launch phases because the technical nature of the development process means fewer opportunities for farmer engagement or pivoting possibilities between these two ends of the product development spectrum. For crop innovation to be relevant to both women and men producers, during the discovery phase, developers can conduct baseline gender analysis consisting of gender-disaggregated value chain analysis, systematic learning about gender-specific crop trait preferences, and identification of varietal preferences by women and men along the value chain. The latter opportunity in the GM product development pathway for intentional gender integration is deployment, including pre-launch activities such as field demonstrations and consumer testing. We also describe ex ante and ex post gender impact assessment methods. We conclude with a number of gender integration recommendations for GM product developers: improving gender data collection and analysis to inform crop innovation efforts, investing in staffing and training of scientific teams to enhance gender expertise, and increasing accountability of product development teams with gender-intentional monitoring and evaluation systems.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"400-410"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and functional analysis of ZmMADS42 gene in maize.","authors":"Yang Zhao, Jianyu Lu, Bo Hu, Peng Jiao, Bai Gao, Zhenzhong Jiang, Siyan Liu, Shuyan Guan, Yiyong Ma","doi":"10.1080/21645698.2024.2328384","DOIUrl":"10.1080/21645698.2024.2328384","url":null,"abstract":"<p><p>Maize (<i>Zea mays</i> L.) is the most important cereal crop in the world. Flowering period and photoperiod play important roles in the reproductive development of maize. This study, investigated <i>ZmMADS42</i>, a gene that is highly expressed in the shoot apical meristem. <i>Agrobacterium</i> infection was used to successfully obtain overexpressed <i>ZmMADS42</i> plants. Fluorescence quantitative PCR revealed that the expression of the <i>ZmMADS42</i> gene in the shoot apical meristem of transgenic plants was 2.8 times higher than that of the wild-type(WT). In addition, the expression of the ZmMADS42 gene in the endosperm was 2.4 times higher than that in the wild-type. The seed width of the T2 generation increased by 5.35%, whereas the seed length decreased by 7.78% compared with that of the wild-type. Dissection of the shoot tips of transgenic and wild-type plants from the 7-leaf stage to the 9-leaf stage revealed that the transgenic plants entered the differentiation stage earlier and exhibited more tassel meristems during their vegetative growth period. The mature transgenic plants were approximately 20 cm shorter in height and had a lower panicle position than the wild-type plants. Comparing the flowering period, the tasseling, powdering, and silking stages of the transgenic plants occurred 10 days earlier than those of the wild-type plants. The results showed that the <i>ZmMADS42</i> gene played a significant role in regulating the flowering period and plant height of maize.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"105-117"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10936638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico analysis and transformation of OsMYB48 transcription factor driven by CaMV35S promoter in model plant - <i>Nicotiana tabacum</i> L. conferring abiotic stress tolerance.","authors":"Yumna Ahmad, Saqlain Haider, Javed Iqbal, Sana Naseer, Kotb A Attia, Arif Ahmed Mohammed, Sajid Fiaz, Tariq Mahmood","doi":"10.1080/21645698.2024.2334476","DOIUrl":"10.1080/21645698.2024.2334476","url":null,"abstract":"<p><p>Global crop yield has been affected by a number of abiotic stresses. Heat, salinity, and drought stress are at the top of the list as serious environmental growth-limiting factors. To enhance crop productivity, molecular approaches have been used to determine the key regulators affecting stress-related phenomena. MYB transcription factors (TF) have been reported as one of the promising defensive proteins against the unfavorable conditions that plants must face. Different roles of MYB TFs have been suggested such as regulation of cellular growth and differentiation, hormonal signaling, mediating abiotic stress responses, etc. To gain significant insights, a comprehensive in-silico analysis of OsMYB TF was carried out in comparison with 21 dicot MYB TFs and 10 monocot MYB TFs. Their chromosomal location, gene structure, protein domain, and motifs were analyzed. The phylogenetic relationship was also studied, which resulted in the classification of proteins into four basic groups: groups A, B, C, and D. The protein motif analysis identified several conserved sequences responsible for cellular activities. The gene structure analysis suggested that proteins that were present in the same class, showed similar intron-exon structures. Promoter analysis revealed major cis-acting elements that were found to be responsible for hormonal signaling and initiating a response to abiotic stress and light-induced mechanisms. The transformation of OsMYB TF into tobacco was carried out using the <i>Agrobacterium</i>-mediated transformation method, to further analyze the expression level of a gene in different plant parts, under stress conditions. To summarize, the current studies shed light on the evolution and role of OsMYB TF in plants. Future investigations should focus on elucidating the functional roles of MYB transcription factors in abiotic stress tolerance through targeted genetic modification and CRISPR/Cas9-mediated genome editing. The application of omics approaches and systems biology will be indispensable in delineating the regulatory networks orchestrated by MYB TFs, facilitating the development of crop genotypes with enhanced resilience to environmental stressors. Rigorous field validation of these genetically engineered or edited crops is imperative to ascertain their utility in promoting sustainable agricultural practices.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"130-149"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The recent genetic modification techniques for improve soil conservation, nutrient uptake and utilization.","authors":"Gideon Sadikiel Mmbando, Kelvin Ngongolo","doi":"10.1080/21645698.2024.2377408","DOIUrl":"10.1080/21645698.2024.2377408","url":null,"abstract":"<p><p>Advances in genetic modification (GM) techniques have generated huge interest in improving nutrient utilization, maximizing nutrient uptake, and conserving soil in the pursuit of sustainable agriculture. Unfortunately, little is still known about the recent advancements in the application of GM tactics to enhance each of these areas. This review explores the latest GM strategies intended to support soil conservation, maximize nutrient uptake, and improve nutrient utilization in farming, highlighting the critical roles that soil health and nutrient management play in sustainable farming. GM strategies such as improving the efficiency of nutrient uptake through enhanced root systems and increased nutrient transport mechanisms are well discussed. This study suggests that addressing potential obstacles, such as ethical and regulatory concerns, is a necessity for long-term sustainability applications of GM technologies to raise agricultural yields.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"233-247"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141621777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}