Maize 4-coumarate coenzyme A ligase Zm4CL-like9 gene positively regulates drought stress response in Arabidopsis thaliana.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2025-12-01 Epub Date: 2025-02-23 DOI:10.1080/21645698.2025.2469942

Jiayi Fan, Zhipeng Luo, Yuankai Wang, Peng Jiao, Qingxu Wang, Yuntao Dai, Shuyan Guan, Yiyong Ma, Huiwei Yu, Siyan Liu

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Abstract

Maize is a major food crop in China, and drought is one of the major abiotic stresses that threaten the growth and development of the crop, seriously affecting the crop yield. 4-coumaric acid coenzyme A ligase (4CL) is a key enzyme in the phenylpropane metabolic pathway, which can regulate the lignin content of the plant and play an important role in the plant's resistance to drought stress, plays an important role in plant resistance to drought stress. In the present study, we screened the differentially expressed up-regulated gene Zm4CL-like9 under drought stress by pre-transcriptome sequencing data (PRJNA793522) in the laboratory, and analyzed the significant up-regulation of Zm4CL-like9 gene in roots under drought stress by qRT-PCR(Real-Time Quantitative Reverse Transcription PCR). The results of prokaryotic expression experiments showed that the protein encoded by the Zm4CL-like9 gene was able to be expressed in prokaryotic cells and could effectively improve the drought tolerance of E. coli. Phenotypic analysis of transgenic Arabidopsis plants under drought stress revealed that seed germination rate, root length, and plant survival after drought rehydration were significantly higher in transgenic Zm4CL-like9 Arabidopsis compared with wild-type Arabidopsis; physiological and biochemical indexes revealed that peroxidase activity, proline (Pro) content, and chlorophyll content were significantly higher in transgenic Arabidopsis compared with wild-type Arabidopsis. Under drought stress, the expression of drought-related genes was significantly up-regulated in transgenic Arabidopsis compared with wild-type Arabidopsis. Taken together, the Zm4CL-like9 gene enhances plant resistance to drought stress by reducing reactive oxygen species accumulation in plants.

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments