Gm Crops & Food-Biotechnology in Agriculture and the Food Chain最新文献_第2页

Rapid recombinase polymerase amplification-based detection of the bar gene for environmental monitoring of genetically modified soybean and rice. 基于快速重组酶聚合酶扩增的bar基因检测在转基因大豆和水稻环境监测中的应用。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-10-08 DOI: 10.1080/21645698.2026.2744012

Areum Jeong, Sung-Dug Oh, Sara Noh, Hong Min, Jong Chan Park, Dohyeon Kwon, Bumkyu Lee

{"title":"Rapid recombinase polymerase amplification-based detection of the bar gene for environmental monitoring of genetically modified soybean and rice.","authors":"Areum Jeong, Sung-Dug Oh, Sara Noh, Hong Min, Jong Chan Park, Dohyeon Kwon, Bumkyu Lee","doi":"10.1080/21645698.2026.2744012","DOIUrl":"10.1080/21645698.2026.2744012","url":null,"abstract":"Rapid and reliable methods are required for environmental monitoring of unintended releases of genetically modified (GM) crops. We developed a real-time recombinase polymerase amplification (RPA) assay targeting the bar gene for the rapid detection of GM soybean and rice, combined with a simplified NaOH-based DNA extraction method for field applications. Primer-probe set 4 provided the most specific and reliable detection, while soybean lectin and rice actin served as internal positive controls. Lectin and actin were consistently detected in GM and non-GM samples, respectively. A 0.1 N NaOH extraction method produced DNA of sufficient quality for direct real-time RPA without additional purification. The integrated assay specifically detected the bar gene in GM samples, with no amplification observed in non-GM samples, and generated results within approximately 25 min at 39 °C. This assay provides a rapid, simple, and field-deployable approach for the detection and environmental monitoring of GM soybean and rice.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2744012"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and regulatory approval of Kal91.3: combining advanced conventional breeding with genetic engineering to create a cold sweetening‑resistant chip‑processing potato. Kal91.3的开发和监管批准：将先进的常规育种与基因工程相结合，创造出抗冷甜的薯片加工马铃薯。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-07-28 DOI: 10.1080/21645698.2026.2707844

Kelly A Zarka, Nanye Long, Joseph Coombs, David S Douches

{"title":"Development and regulatory approval of Kal91.3: combining advanced conventional breeding with genetic engineering to create a cold sweetening‑resistant chip‑processing potato.","authors":"Kelly A Zarka, Nanye Long, Joseph Coombs, David S Douches","doi":"10.1080/21645698.2026.2707844","DOIUrl":"10.1080/21645698.2026.2707844","url":null,"abstract":"Globally, potato (Solanum tuberosum) is an important food crop. Due to an expanding potato‑chip processing market, it is increasingly essential that high-quality raw tubers are maintained and available year-round. Cold storage at temperatures below 10°C greatly increases the duration of potato tuber storage, however, potatoes are susceptible to cold-induced sweetening (CIS). At these temperatures, potato starch stored in the tuber converts to reducing sugars, leading to a dark, bitter-tasting potato chip. Kalkaska is a potato variety with high yield and a highly desirable scab resistance; however, it is unreliable in managing reducing sugars in commercial storage. In this study, we report a comprehensive characterization and analysis of a genetically engineered potato line Kal91.3, utilizing VInv RNAi suppression to correct the reducing sugar management in Kalkaska. A full molecular characterization analysis was completed on Kal91.3, which demonstrated that there are two independent and stable T-DNA insertions. RNA analysis reported suppression of the VInv transcripts, which was further shown during a prolonged tuber storage period. Kalkaska and Kal91.3 tubers were stored at 4°C for up to 8 months. Kal91.3 produced chips light in color, and the Kalkaska control produced dark chips typical of the CIS darkening response. Kal91.3 is the first genetically engineered field‑crop product developed by a non-industry organization and to have received regulatory clearance in the United States. Kal91.3 is expected to provide significant benefits to growers, processors, and consumers by producing high-quality potato chips from long-term, lower-temperature storage, improving supply‑chain stability, and waste reduction.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2707844"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148610087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of GMO media strategies on public perceptions of CRISPR crop technologies in Southern Ontario. 转基因媒体策略对南安大略省公众对CRISPR作物技术认知的影响

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-02-11 DOI: 10.1080/21645698.2026.2620131

Poornima Goudar, Alexander Hall

{"title":"The influence of GMO media strategies on public perceptions of CRISPR crop technologies in Southern Ontario.","authors":"Poornima Goudar, Alexander Hall","doi":"10.1080/21645698.2026.2620131","DOIUrl":"10.1080/21645698.2026.2620131","url":null,"abstract":"Genetically modified organisms (GMOs) have often divided public opinion, one factor influencing perceptions of GMO technologies has been misunderstood or poorly communicated scientific messaging. However, advancements in gene editing tools like CRISPR/Cas9 offer new crop modification possibilities, prompting different regulatory frameworks than traditional GMO technologies. This research examines public understanding of GMOs, awareness of CRISPR crops, and how prior experiences with GMOs shape perceptions of new genetic technologies. A mixed-methods approach was employed, combining a public survey of adults in the Greater Toronto-Hamilton area and interviews with science journalists. Results show hesitance toward GMOs and CRISPR crops, with acceptance most impacted by consumer behavior and cost. Key interview themes include journalist's concerns about levels of public education, the role of social media, and the cost of goods. Our findings suggest increased transparency and effective communication could improve public acceptance of GMOs and CRISPR crops. While CRISPR crops do not come under the GMO regulatory framework in Canada, our findings show that the public does not recognize this distinction. Without increased transparency and more effective communication CRISPR crops may become widely associated with the negative media frames that have shaped perceptions of GMOs.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2620131"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12915814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events. 简单、快速、可靠：19个转基因大豆事件的多重数字PCR定量。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-02-27 DOI: 10.1080/21645698.2026.2635816

Amadej Jelenčič, Dejan Štebih, Tina Demšar, David Dobnik

{"title":"Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.","authors":"Amadej Jelenčič, Dejan Štebih, Tina Demšar, David Dobnik","doi":"10.1080/21645698.2026.2635816","DOIUrl":"10.1080/21645698.2026.2635816","url":null,"abstract":"Plant genetic engineering represents an important aspect of modern agriculture, and new genetically modified (GM) crop varieties are entering the market on a regular basis. This necessitates the development of high throughput multi-target analytical methods to detect and quantify their presence for regulatory compliance. In this study, we present a multiplex dPCR method for discriminative quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of the platform's multiple fluorescence detection channels. The assays complied with the minimum performance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range, making them suitable for use in routine detection and quantification of GM crops. This method represents the most comprehensive multi-target GM soybean quantification approach to date without the need for prior screening and features a simplified workflow, making it suitable for widespread adoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCR GM crop quantification assays to address the evolving demands of regulatory monitoring.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2635816"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12959185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147318771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends and intellectual property landscape in soybean breeding: a patent and paper Bibliometric analysis. 大豆育种的趋势和知识产权格局：专利和论文文献计量学分析。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-05-05 DOI: 10.1080/21645698.2026.2662120

Maria Fernanda Sua-Rojas, Daniel Neris, Marcelo Menossi

{"title":"Trends and intellectual property landscape in soybean breeding: a patent and paper Bibliometric analysis.","authors":"Maria Fernanda Sua-Rojas, Daniel Neris, Marcelo Menossi","doi":"10.1080/21645698.2026.2662120","DOIUrl":"10.1080/21645698.2026.2662120","url":null,"abstract":"Soybean [Glycine max (L.) Merr.] is one of the most important crops globally. Researchers have focused on enhancing soybean through genetic modification, leading to an extensive accumulation of publications and patents. Despite this, a comprehensive exploration of this information in breeding research has been lacking. Using data from the Web of Science (WoS) and Orbit Intelligence databases (2013-2023), this study employs the Bibliometrix package in R and Gephi for visual analysis. We examined keywords, co-occurrences, collaborations, and emerging trends to shed light on future research and intellectual property (IP) directions. The findings reveal a consistent rise in annual publications, in contrast to a decline in patent filings. China and the United States emerge as the primary contributors. Analysis of patent records identifies M S Technologies and Monsanto Technology LLC as key players. This study provides a vital reference for researchers and industry stakeholders to address global food security challenges.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2662120"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of public perception and socio-demographic drivers of genetically modified organisms in Iran. 伊朗公众对转基因生物的看法和社会人口驱动因素分析。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-05-07 DOI: 10.1080/21645698.2026.2668245

Mohammad Ahmadabadi, Zahra Dehghanian, Rana Valizadeh Kamran, Maghsoud Pazhouhandeh, Ali Aryan

{"title":"Analysis of public perception and socio-demographic drivers of genetically modified organisms in Iran.","authors":"Mohammad Ahmadabadi, Zahra Dehghanian, Rana Valizadeh Kamran, Maghsoud Pazhouhandeh, Ali Aryan","doi":"10.1080/21645698.2026.2668245","DOIUrl":"10.1080/21645698.2026.2668245","url":null,"abstract":"The rapid growth in the world's population poses a significant threat to food security, making genetically modified organisms (GMOs) an important potential solution. Alongside technological advancements, public awareness of GMOs - including their benefits and existing challenges - plays a crucial role in their acceptance and use within communities. This study aimed to assess opinions and the level of awareness of the Iranian public regarding GM products, the sources they consult for information on the topic, and their trust in these sources. A survey was conducted with 5,730 predominantly young, urban, and well-educated individuals from 16 provinces. The results revealed that 40% of respondents held positive attitudes toward GM crops, a figure higher than those reported in some social media analyses. Using multiple logistic regression, the study found that factors such as age, education, occupation, and residence significantly influenced attitudes toward GMOs, whereas gender did not. Although 42% of participants reported having limited knowledge about GMOs, most expressed greater trust in scientists compared to media sources and supported various applications of GMO technology. These findings, which focus on educated urban youth due to convenience sampling, underscore the importance of education and media in shaping perceptions of GM products in Iran.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2668245"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13166234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Operationalizing insect resistance management for commercialized Bt crops: an evidence-to-action framework for post-market stewardship. 商业化Bt作物抗虫管理的实施：上市后管理的循证行动框架。

IF 4.3 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2026-12-01 Epub Date: 2026-07-27 DOI: 10.1080/21645698.2026.2709923

Zhanfeng Yan, Dapeng Jing, Zhenying Wang

{"title":"Operationalizing insect resistance management for commercialized Bt crops: an evidence-to-action framework for post-market stewardship.","authors":"Zhanfeng Yan, Dapeng Jing, Zhenying Wang","doi":"10.1080/21645698.2026.2709923","DOIUrl":"10.1080/21645698.2026.2709923","url":null,"abstract":"Commercialized Bacillus thuringiensis (Bt) crops require post-market stewardship that translates heterogeneous resistance signals into defensible action. Although previous syntheses have defined field-evolved and practical resistance and summarized global patterns, developers, regulators, and stewardship programs still need an operational structure for distinguishing weak anomalies from evidence warranting confirmation, mitigation, deployment revision, or replacement of compromised components. We synthesize evidence and stewardship experience from Bt maize and Bt cotton into an evidence-to-action framework linking preventive IRM implementation, resistance monitoring, evidence-state assignment, actionability assessment, and proportionate response. The framework classifies outcomes into four states: baseline susceptibility, early-warning signal, confirmed field-evolved resistance, and practical resistance. For each state, we define its operational meaning, minimum verification requirements, and proportionate actions. Representative pest - crop - toxin systems illustrate how field performance, bioassays, mechanistic evidence, deployment history, and refuge context can support earlier, more transparent, and evidence-proportionate stewardship decisions.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"17 1","pages":"2709923"},"PeriodicalIF":4.3,"publicationDate":"2026-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13418707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148594661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rate of herbicide resistant weed development: A Canadian Prairie case study. 抗除草剂杂草发展速度：加拿大草原案例研究。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2025-12-01 Epub Date: 2025-03-09 DOI: 10.1080/21645698.2025.2477231

Chelsea Sutherland, Savannah Gleim, Simona Lubieniechi, Stuart J Smyth

{"title":"Rate of herbicide resistant weed development: A Canadian Prairie case study.","authors":"Chelsea Sutherland, Savannah Gleim, Simona Lubieniechi, Stuart J Smyth","doi":"10.1080/21645698.2025.2477231","DOIUrl":"10.1080/21645698.2025.2477231","url":null,"abstract":"Genetically modified crop adoption in Canada has been the key driver in removing tillage as the lead form of weed control, due to increased weed control efficiency. Land use has transitioned from the use of summerfallow to continuous cropping, predominantly involving zero or minimum tillage practices. Prairie crop rotations have diversified away from mainly cereals to include three-year rotations of cereals, pulses, and oilseeds. Total herbicide volume applied has increased as crop production acres increased, but the rate of herbicide active ingredient applied per hectare has declined. Diverse crop rotations allow for weed control using herbicides with different modes of action, reducing selection pressure for resistant weed development. Herbicide-resistant weeds are an important concern for farmers, as the loss of key herbicides would make weed control exceedingly more difficult. The objective of this case study is to examine herbicide resistance weed development in the Canadian Prairies and to identify changes in resistance development following GM crop adoption.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"252-262"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Science-based safety assessment of genetically modified DP915635 maize. 转基因玉米DP915635的科学安全性评价。

IF 4.7 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2025-12-01 Epub Date: 2024-12-31 DOI: 10.1080/21645698.2024.2439116

Kent Brink, Tim Gunderson, Matthew Harmon, Kristine LeRoy, Margit Ross, Anitha S C, Brian Stolte, John Zhang

{"title":"Science-based safety assessment of genetically modified DP915635 maize.","authors":"Kent Brink, Tim Gunderson, Matthew Harmon, Kristine LeRoy, Margit Ross, Anitha S C, Brian Stolte, John Zhang","doi":"10.1080/21645698.2024.2439116","DOIUrl":"10.1080/21645698.2024.2439116","url":null,"abstract":"Genetically modified maize event DP-915635-4 expressing the IPD079Ea protein was developed to control corn rootworm damage to maize plants. Utilizing a modernized safety assessment model published by CropLife International (CLI), the safety of DP-915635-4 maize was assessed. The CLI core studies included were molecular characterization of the inserted DNA, expression and characterization of the expressed IPD079Ea protein, and its safety for both food and feed use and the environment. No hazards were identified for human or animal consumption of DP-915635-4 maize containing the IPD079Ea protein, indicating that supplementary studies were not necessary. An environmental risk assessment was performed to characterize any potential impacts to non-target organisms, the results of which established that DP-915635-4 maize is unlikely to result in unreasonable adverse effects to non-target organisms. This case study shows that the modernized safety assessment is effective at demonstrating the safety of genetically modified crop plants.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"72-82"},"PeriodicalIF":4.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome and nutritional composition analysis of stacked transgenic maize with insect resistance and herbicide tolerance. 抗虫抗除草剂转基因玉米堆叠体转录组和营养成分分析。

IF 4.5 2区农林科学

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2025-12-01 Epub Date: 2025-02-27 DOI: 10.1080/21645698.2025.2472451

Xiaoxing Yu, Hongyu Gao, Pengfei Wang

{"title":"Transcriptome and nutritional composition analysis of stacked transgenic maize with insect resistance and herbicide tolerance.","authors":"Xiaoxing Yu, Hongyu Gao, Pengfei Wang","doi":"10.1080/21645698.2025.2472451","DOIUrl":"10.1080/21645698.2025.2472451","url":null,"abstract":"The safety assessment of stacked transgenic crops is essential for their commercial cultivation. A crucial element of safety assessment is the nutritional evaluation of transgenic crops. Currently, profiling methods like transcriptome are employed as supplemental analytical tools to find the unintended effects of transgenic crops. In this study, stacked transgenic maize ZDRF8×nCX-1 was produced by crossing of two transgenic maize events ZDRF8 and nCX-1. This stacked transgenic maize expresses five genes: cry1Ab, cry2Ab and g10evo-epsps (from ZDRF8), as well as cp4 epsps and P450-N-Z1 (from nCX-1). Molecular analysis showed that the insertion sites of target genes were not changed during stack breeding, and the target genes are effectively expressed at both RNA and protein levels in ZDRF8×nCX-1. Target trait analysis showed that ZDRF8×nCX-1 exhibits tolerant to glyphosate, flazasulfuron and MCPA, and is resistant to damage by corn borers. Transcriptome analysis revealed that gene-stacked maize ZDRF8×nCX-1 did not significantly alter transcriptome profiles compared to the transgenic maize events ZDRF8 and nCX-1. Nutritional composition analysis showed that the grain profile of ZDRF8×nCX-1 was substantially equivalent to that of the non-transgenic counterpart. These results suggest that hybrid stacking does not cause significantly unintended effects beyond providing the intended beneficial traits.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"216-234"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0