Gm Crops & Food-Biotechnology in Agriculture and the Food Chain最新文献

{"title":"Characterization of glyphosate-tolerant genetically modified eucalyptus.","authors":"Antonio Carlos Mota Porto, José Mateus Wisniewski Gonsalves, Paula Aparecida Vieira, Matheus Perek, Diego da Costa Lima, Marcio Nagayschi, Thais Regina Drezza, Ana Cristina Pinheiro, Eduardo Jose de Mello, Dror Avisar, Rodrigo Neves Graca","doi":"10.1080/21645698.2024.2429200","DOIUrl":"10.1080/21645698.2024.2429200","url":null,"abstract":"<p><p>Eucalyptus stands out as one of the most productive tree species for large-scale cultivation. However, like all cultivated crops, it requires specialized management practices, including the control of weeds, pathogens, and pests. Glyphosate is the most widely applied herbicide used in the essential weeding effort, and it ensures the sustainable management of eucalyptus cultivation in Brazil. Given the sensitivity of eucalyptus to glyphosate, existing weed control methods in young eucalyptus farms predominantly rely on protected mechanical or/and knapsack spraying. Both methods contribute to herbicide drift, which compromises tree yield and increases chemical waste due to uneven spraying. This study provides a detailed observation of the physiological parameters and long-term field performance of glyphosate-tolerant (HT), genetically modified (GM) eucalyptus developed by FuturaGene/Suzano S.A. and approved in Brazil for operational deployment. The HT GM eucalyptus events were meticulously evaluated to ensure high levels of glyphosate tolerance. This involved the direct application of herbicide on seedlings in greenhouse studies and on young trees in field conditions. The herbicide-treated GM eucalyptus in all trials demonstrated consistent growth and maintained physiological parameters comparable to their respective non-sprayed wild-type (WT) counterparts. The HT GM eucalyptus represents a significant advancement by enabling the direct application of glyphosate over the top of the trees to control the weeds within the planting row. This innovative approach minimizes the need for frequent mechanical and manual interventions, thereby lowering worker herbicide exposure, reducing the environmental impact of mechanical operations, and enhancing the overall efficiency and sustainability of HT GM eucalyptus stands.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"361-373"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Golden opportunities? How marketing expectations drive purchase intentions of golden rice in Bangladesh and the Philippines.","authors":"Dilshad Zahan Ethen, Maimuna Begum, Berre Deltomme, Md Rasheduzzaman, Josefina F Ballesteros, Riza Abilgos-Ramos, Mohammad Jahangir Alam, Alice Onek Atimango, Hans De Steur","doi":"10.1080/21645698.2024.2418161","DOIUrl":"10.1080/21645698.2024.2418161","url":null,"abstract":"<p><p>Golden Rice (GR), genetically modified (GM) rice enriched with provitamin A, holds promise to address micronutrient deficiencies in developing countries. However, its success hinges on market acceptance. This study investigates how the marketing aspects of GR influence consumers' purchase intentions in Bangladesh and the Philippines. The Expectation Confirmation Theory (ECT) is employed to analyze the role of expectations regarding the marketing mix components (i.e. product, price, place, promotion), risk perceptions, performance expectations, and expected satisfaction on consumers' purchase intentions. Data from online surveys in Bangladesh (<i>n</i> = 391) and the Philippines (<i>n</i> = 354), collected using convenience sampling, were analyzed using structural equation modeling. Findings reveal that positive expectations toward the marketing mix, performance, and satisfaction increase consumers' purchase intention of GR, whereas risk perceptions have a negative influence. Additionally, it was found that expectations toward all four marketing mix components significantly affect purchase intention in Bangladesh. However, only product and promotion have a notable influence in the Philippines. These results emphasize the importance of effectively addressing consumers' marketing expectations to help ensure a successful implementation. This study is novel as it delves into consumers' purchase intentions for a GM biofortified crop and their expectations for different aspects of its future marketing (i.e. product, price, place, promotion), performance, and satisfaction. If GR is commercialized, future research should validate these expectations based on actual consumer experiences. Additionally, longitudinal studies could track changes in consumer expectations over time, identifying consistently valued marketing elements and offering a valuable technique for product development before launch.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"316-335"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome editing in Sub-Saharan Africa: a game-changing strategy for climate change mitigation and sustainable agriculture.","authors":"Peter Amoah, Abdoul-Razak Oumarou Mahamane, Moise Hubert Byiringiro, Neo Jeremiah Mahula, Nyimasata Manneh, Yetunde Ruth Oluwasegun, Abebawork Tilahun Assfaw, Hellen Mawia Mukiti, Abubakar Danlami Garba, Felicity Kido Chiemeke, Omena Bernard Ojuederie, Bunmi Olasanmi","doi":"10.1080/21645698.2024.2411767","DOIUrl":"10.1080/21645698.2024.2411767","url":null,"abstract":"<p><p>Sub-Saharan Africa's agricultural sector faces a multifaceted challenge due to climate change consisting of high temperatures, changing precipitation trends, alongside intensified pest and disease outbreaks. Conventional plant breeding methods have historically contributed to yield gains in Africa, and the intensifying demand for food security outpaces these improvements due to a confluence of factors, including rising urbanization, improved living standards, and population growth. To address escalating food demands amidst urbanization, rising living standards, and population growth, a paradigm shift toward more sustainable and innovative crop improvement strategies is imperative. Genome editing technologies offer a promising avenue for achieving sustained yield increases while bolstering resilience against escalating biotic and abiotic stresses associated with climate change. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein (CRISPR/Cas) is unique due to its ubiquity, efficacy, alongside precision, making it a pivotal tool for Sub-Saharan African crop improvement. This review highlights the challenges and explores the prospect of gene editing to secure the region's future foods.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"279-302"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing the impact of CPSF30 gene disruption on TuMV infection in <i>Arabidopsis thaliana</i>.","authors":"Yanping Wei, Quan Yuan, Dalal Sulaiman Alshaya, Abdul Waheed, Kotb A Attia, Sajid Fiaz, Muhammad Shahid Iqbal","doi":"10.1080/21645698.2024.2403776","DOIUrl":"10.1080/21645698.2024.2403776","url":null,"abstract":"<p><p>CPSF30, a key polyadenylation factor, also serves as an m⁶A reader, playing a crucial role in determining RNA fate post-transcription. While its homologs mammals are known to be vital for viral replication and immune evasion, the full scope of CPSF30 in plant, particular in viral regulation, remains less explored. Our study demonstrates that CPSF30 significantly facilitates the infection of turnip mosaic virus (TuMV) in <i>Arabidopsis thaliana</i>, as evidenced by infection experiments on the engineered <i>cpsf30</i> mutant. Among the two isoforms, CPSF30-L, which were characterized with m⁶A binding activity, emerged as the primary isoform responding to TuMV infection. Analysis of m⁶A components revealed potential involvement of the m⁶A machinery in regulating TuMV infection. In contrast, CPSF30-S exhibited distinct subcellular localization, coalescing with P-body markers (AtDCP1 and AtDCP2) in cytoplasmic granules, suggesting divergent regulatory mechanisms between the isoforms. Furthermore, comprehensive mRNA-Seq and miRNA-Seq analysis of Col-0 and <i>cpsf30</i> mutants revealed global transcriptional reprogramming, highlighting CPSF30's role in selectively modulating gene expression during TuMV infection. In conclusion, this research underscores CPSF30's critical role in the TuMV lifecycle and sets the stage for further exploration of its function in plant viral regulation.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"1-17"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142332351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of media reportage on GMOs in Ghana following approval of first GM crop.","authors":"Joseph Opoku Gakpo, Dennis Baffour-Awuah","doi":"10.1080/21645698.2024.2365481","DOIUrl":"10.1080/21645698.2024.2365481","url":null,"abstract":"<p><p>Ghana's parliament in 2011 passed the Biosafety Act to allow for the application of genetically modified organism (GMO) technology in the country's agriculture. In a vibrant democracy, there have been extensive media discussions on whether GM crops will benefit or harm citizens. In June 2022, the state GMO regulator, the National Biosafety Authority (NBA), approved the country's first GM crop (Bt cowpea) for environmental release, declaring the crop does not present an altered environmental risk or a food/feed safety concern. This study identified 3 of the country's most vibrant digital news outlets and did a content analysis of all GMO stories reported 18 months pre- and post-approval to assess whether the approval changed the focus of GMO issues the media reports on. 91 articles were identified. The results show media reports on the likely impact of GMOs on the country's food security shot up after the approval. However, media reports on the possible health, sociocultural, and environmental impact of GMOs declined. We observe the media and the public appear interested in deliberations on how the technology could address or worsen food insecurity and urge agricultural biotechnology actors in Ghana to focus on that in their sensitization activities.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"16-27"},"PeriodicalIF":3.9,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An environmental risk assessment of IPD079Ea: a protein derived from <i>Ophioglossum pendulum</i> with activity against <i>Diabrotica</i> spp.In maize.","authors":"Bridget F O'Neill, Chad Boeckman, Kristine LeRoy, Chris Linderblood, Taylor Olson, Rachel Woods, Mary Challender","doi":"10.1080/21645698.2023.2299503","DOIUrl":"10.1080/21645698.2023.2299503","url":null,"abstract":"<p><p>Farmers in North America face significant pressure from insects in their maize fields, particularly from corn rootworm (<i>Diabrotica</i> spp.). Research into proteins capable of insecticidal activity has found several produced by ferns. One protein, IPD079Ea, was derived from <i>Ophioglossum pendulum</i> and has shown activity against corn rootworm. An environmental risk assessment was conducted for maize event DP-915635-4, which provides control of corn rootworms via expression of the IPD079Ea protein. This assessment focused on IPD079Ea and characterized potential exposure and hazard to non-target organisms (NTOs). For exposure, estimated environmental concentrations (EECs) were calculated. For hazard, laboratory dietary toxicity studies were conducted with IPD079Ea and surrogate non-target organisms. Environmental risk was characterized by comparing hazard and exposure to calculate the margin of exposure (MOE). Based on the MOE values for DP-915635-4 maize, the IPD079Ea protein is not expected to result in unreasonable adverse effects on beneficial NTO populations at environmentally relevant concentrations.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"15-31"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best practices for acceptability of GM crops field trials conclusions: lessons for Africa.","authors":"Paul Chege, Julia Njagi, John Komen, Godfrey Ngure, John Muriuki, Margaret Karembu","doi":"10.1080/21645698.2024.2376415","DOIUrl":"10.1080/21645698.2024.2376415","url":null,"abstract":"<p><p>The ability to transfer information about the performance, safety, and environmental impacts of a genetically modified (GM) crop from confined field trials (CFTs) conducted in one location to another is increasingly gaining importance in biosafety regulatory assessment and decision-making. The CFT process can be expensive, time-consuming, and logistically challenging. Data transportability can help overcome these challenges by allowing the use of data obtained from CFTs conducted in one country to inform regulatory decision-making in another country. Applicability of transported CFT data would be particularly beneficial to the public sector product developers and small enterprises that develop innovative GM events but cannot afford to replicate redundant CFTs, as well as regulatory authorities seeking to improve the deployment of limited resources. This review investigates case studies where transported CFT data have successfully been applied in biosafety assessment and decision-making, with an outlook of how African countries could benefit from a similar approach.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"222-232"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11236291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does DNA extraction affect the specificity of a PCR method claiming the specific detectability of a genome-edited plant?","authors":"Sophia Edelmann, Christian Savini, Dominik Moor, Jörn Lämke, Kathrin Lieske, Marco Mazzara, Hendrik Emons, Joachim Mankertz, Christopher Weidner","doi":"10.1080/21645698.2024.2423441","DOIUrl":"10.1080/21645698.2024.2423441","url":null,"abstract":"<p><p>Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America. In a previous study, we have independently assessed this method in three reference laboratories for sensitivity, specificity, and robustness. We found that the method does not meet all the minimum performance requirements (MPR) for GMO testing in the EU, which contradicts the claims of the method developer. Here we show, in addition to the previously published method assessment study that a modified DNA extraction is not the reason for the contradictory findings and does not affect the specificity of the method. We also discuss the procedures recently proposed by the method developers for interpreting PCR results with high Cq values.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"352-360"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumers' response to genetically modified food: an Italian case study.","authors":"Federica DeMaria, Simona Romeo Lironcurti, Federica Morandi, Alessandra Pesce, Laura Gazza, Francesca Nocente","doi":"10.1080/21645698.2024.2417473","DOIUrl":"10.1080/21645698.2024.2417473","url":null,"abstract":"<p><p>The agricultural sector could benefit from biotechnologies in addressing challenges such as pests, droughts, and food supply issues. Genetically modified (GM) crops have been developed to offer not only economic advantages to farmers but also to contribute positively to the environment, human health, and consumer well-being. However, consumers' hesitancy in buying GM food may stem from societal reactions to how biotechnologies in agriculture have been regulated so far. The legislative debate that led, in early 2024, to the approval of Commission's proposal (COM(2023) 411 final) - aimed at simplifying the authorization process for plants obtained with certain new genomic techniques (NGTs) - has sparkled public discussion in the European Union on the application of biotechnologies in agriculture. This work aims to investigate Italian consumers' acceptance toward GM food. Through data collected from an original survey (<i>N</i> = 564), we tested a) their level of knowledge of GM techniques; b) if they are aware of differences between established techniques based on classical approaches of crossing and selection and more precise biotechnological techniques; c) their propensity to buy GM food, with a specific focus on food safety and environmental sustainability issues. By using a Multinomial Logit Model (MNL), starting from three hypotheses, the study highlights a gap in knowledge transfer and, in general, the communication process. This results in widespread misinformation that hinders informed consumer choices. The study also emphasizes consumers' sensitivity to food safety, including environmental issues, but still related to food safety issues.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"303-315"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of accelerating agricultural R&D transfer on global food security.","authors":"Yan Jin, Zuzana Smeets Kristkova, Maximilian Kardung, Justus Wesseler","doi":"10.1080/21645698.2024.2438419","DOIUrl":"10.1080/21645698.2024.2438419","url":null,"abstract":"<p><p>Postponing the adoption of genome editing (GE) is costly, with lengthy regulatory processes contributing to postponement. Accelerating agricultural research and development (R&D) transfer is important for stimulating sustainable agricultural transitions and enhancing global food security. Using the MAGNET model, we incorporate dynamic R&D accumulation and compare economic projections in scenarios with accelerated R&D transfer. We calculate the cost of delay (COD) from postponing GE adoption. The results show that accelerating R&D transfer in high-income countries impacts economic performance, welfare, and food affordability globally; the annuity of COD ranges from losses of -$1.1 billion (Brazil) to gains of $18.5 billion (Europe). A 3-year acceleration of R&D transfer in all countries benefits middle and low-income countries the most (e.g. China, India, other Asian countries, and Sub-Saharan African countries), with the annuity of COD ranging from -$4.8 billion (Brazil) to $83.9 billion (China). Therefore, streamlining the GE regulatory framework is essential for enhancing food security and global welfare.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"15 1","pages":"1-12"},"PeriodicalIF":4.5,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}