Sujung Park, Sanggu Lee, Soo-In Sohn, Taesung Park, Kongsik Shin
{"title":"Specific detection of genetically modified potatoes containing <i>asparagine synthetase-1</i> and <i>polyphenol oxidase 5</i> genes derived from potato.","authors":"Sujung Park, Sanggu Lee, Soo-In Sohn, Taesung Park, Kongsik Shin","doi":"10.1080/21645698.2025.2488085","DOIUrl":null,"url":null,"abstract":"<p><p>Several genetically modified (GM) potatoes have been developed by introducing endogenous genes derived from potatoes, such as <i>asparagine synthetase-1</i> (<i>Asn1</i>) and <i>polyphenol oxidase 5</i> (<i>Ppo5</i>), to improve quality. Therefore, it is difficult to distinguish between GM and non-GM potatoes. In this study, we developed a sequence-specific polymerase chain reaction (PCR) detection method to identify innate and inserted genes. We designed four <i>Asn1</i> gene-specific primers and eight construct-specific detection primers to evaluate GM potatoes (E12, X17, and Y9) and non-GM crops. Consequently, PCR products corresponding to the original endogenous potato genes and the inserted genes were clearly distinguished and simultaneously identified. In addition, the PCR method demonstrated sufficient sensitivity to identify GM content at levels as low as 0.5%. Thus, this study offers an effective detection method for monitoring or screening approved and unapproved GM potato events using <i>Asn1</i> and <i>Ppo5</i> transgenes in foods and feeds.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"16 1","pages":"318-328"},"PeriodicalIF":4.5000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980468/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21645698.2025.2488085","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Several genetically modified (GM) potatoes have been developed by introducing endogenous genes derived from potatoes, such as asparagine synthetase-1 (Asn1) and polyphenol oxidase 5 (Ppo5), to improve quality. Therefore, it is difficult to distinguish between GM and non-GM potatoes. In this study, we developed a sequence-specific polymerase chain reaction (PCR) detection method to identify innate and inserted genes. We designed four Asn1 gene-specific primers and eight construct-specific detection primers to evaluate GM potatoes (E12, X17, and Y9) and non-GM crops. Consequently, PCR products corresponding to the original endogenous potato genes and the inserted genes were clearly distinguished and simultaneously identified. In addition, the PCR method demonstrated sufficient sensitivity to identify GM content at levels as low as 0.5%. Thus, this study offers an effective detection method for monitoring or screening approved and unapproved GM potato events using Asn1 and Ppo5 transgenes in foods and feeds.
期刊介绍：
GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers.
GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer.
Topics covered include, but are not limited to:
• Production and analysis of transgenic crops
• Gene insertion studies
• Gene silencing
• Factors affecting gene expression
• Post-translational analysis
• Molecular farming
• Field trial analysis
• Commercialization of modified crops
• Safety and regulatory affairs
BIOLOGICAL SCIENCE AND TECHNOLOGY
• Biofuels
• Data from field trials
• Development of transformation technology
• Elimination of pollutants (Bioremediation)
• Gene silencing mechanisms
• Genome Editing
• Herbicide resistance
• Molecular farming
• Pest resistance
• Plant reproduction (e.g., male sterility, hybrid breeding, apomixis)
• Plants with altered composition
• Tolerance to abiotic stress
• Transgenesis in agriculture
• Biofortification and nutrients improvement
• Genomic, proteomic and bioinformatics methods used for developing GM cops
ECONOMIC, POLITICAL AND SOCIAL ISSUES
• Commercialization
• Consumer attitudes
• International bodies
• National and local government policies
• Public perception, intellectual property, education, (bio)ethical issues
• Regulation, environmental impact and containment
• Socio-economic impact
• Food safety and security
• Risk assessments
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
