Patricia V Miranda, Bernardo F Iglesias, María V Charriere, Moisés Burachik
{"title":"Drought tolerant wheat IND-ØØ412-7 is nutritionally equivalent to its Non-Transgenic Comparator.","authors":"Patricia V Miranda, Bernardo F Iglesias, María V Charriere, Moisés Burachik","doi":"10.1080/21645698.2022.2079179","DOIUrl":"https://doi.org/10.1080/21645698.2022.2079179","url":null,"abstract":"<p><p>Expression of the HAHB4 sunflower transcription factor confers drought tolerance to wheat event IND-ØØ412-7 (HB4® wheat). After confirming the compositional equivalence of event IND-ØØ412-7 with conventional wheat, its nutritional similarity to its non-genetically modified (GM) counterpart was analyzed by performing a 42-day broiler feeding study. Isoenergetic diets containing 40% flour from wheat event IND-ØØ412-7, its non-GM counterpart Cadenza, and a commercial variety were included in the study. Broilers' performance was analyzed by measuring feed intake, weight gain, feed conversion, and time to reach 2.8 kgs. The yield was evaluated by carcass weight, breast meat, and abdominal fat. No differences were found between wheat event IND-ØØ412-7 and the non-GM counterpart. A few significant differences were found with the commercial variety which were associated with the genetic background, different from the other two materials. These results support the nutritional equivalence of event IND-ØØ412-7 with conventional wheat.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"13 1","pages":"119-125"},"PeriodicalIF":3.9,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9176220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10321375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sendhil R, Joan Nyika, Sheel Yadav, Joby Mackolil, Rama Prashat G, Endashaw Workie, Raja Ragupathy, P Ramasundaram
{"title":"Genetically modified foods: bibliometric analysis on consumer perception and preference.","authors":"Sendhil R, Joan Nyika, Sheel Yadav, Joby Mackolil, Rama Prashat G, Endashaw Workie, Raja Ragupathy, P Ramasundaram","doi":"10.1080/21645698.2022.2038525","DOIUrl":"10.1080/21645698.2022.2038525","url":null,"abstract":"<p><p>In this study, we present the bibliometric trends emerging from research outputs on consumer perception and preference for genetically modified (GM) foods and policy prescriptions for enabling the consumption using VOSviewer visualization software. Consumers' positive response is largely influenced by the decision of the governments to ban or approve the GM crops cultivation. Similarly, the public support increases when the potential benefits of the technology are well articulated, consumption increases with a price discount, people's trust on the government and belief in science increases with a positive influence by the media. Europe and the USA are the first region and country, respectively, in terms of the number of active institutions per research output, per-capita GDP publication and citations. We suggest research-, agri-food industries-, and society-oriented policies to be implemented by the stakeholders to ensure the safety of GM foods, encourage consumer-based studies, and increase public awareness toward these food products.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"13 1","pages":"65-85"},"PeriodicalIF":4.5,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9009926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41348212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Naglaa A Abdallah, Hany Elsharawy, Hamiss A Abulela, Roger Thilmony, Abdelhadi A Abdelhadi, Nagwa I Elarabi
{"title":"Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat.","authors":"Naglaa A Abdallah, Hany Elsharawy, Hamiss A Abulela, Roger Thilmony, Abdelhadi A Abdelhadi, Nagwa I Elarabi","doi":"10.1080/21645698.2022.2120313","DOIUrl":"10.1080/21645698.2022.2120313","url":null,"abstract":"<p><p>Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3'(2'), 5'-bisphosphate nucleotidase (<i>TaSal1</i>) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active <i>TaSal1</i> homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat <i>TaSal1</i> genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable <i>TaSal1</i> mutations in the M<sub>1</sub> progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and bended stems, but there were no significant changes in the internode length and width, leaf morphology, and stem shape. Anatomical and scanning electron microscope studies of the young leaves of mutated <i>TaSal1</i> lines showed closed stomata, increased stomata width and increase in the size of the bulliform cells. <i>Sal1</i> mutant seedlings germinated and grew better on media containing polyethylene glycol than wildtype seedlings. Our results indicate that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing is efficient tool for mutating more multiple TaSal1 loci in hexaploid wheat.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":" ","pages":"1-17"},"PeriodicalIF":3.9,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33490173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biotechnology in China – regulation, investment, and delayed commercialization","authors":"Z. Xiao, W. Kerr","doi":"10.1080/21645698.2022.2068336","DOIUrl":"https://doi.org/10.1080/21645698.2022.2068336","url":null,"abstract":"ABSTRACT China has been investing heavily in biotechnology to increase agricultural productivity. While a number of Chinese developed GM crops have cleared the required scientific hurdles – some more than a decade ago – commercialization has not been approved. The regulatory regime for GMOs in China is relatively less well understood than that of the US or the EU. This paper provides a systematic overview of China’s regulatory regime, R&D investment and delayed commercialization decisions on biotechnology over the last 40 years and draws some conclusions regarding the likelihood of the commercialization for major GM crops in the future.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"13 1","pages":"86 - 96"},"PeriodicalIF":3.9,"publicationDate":"2022-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49246428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modeling antecedent factors involved in behavioral intention towards technology application of genetically modified crops","authors":"Yahya Safi Sis, A. Rezaei, Hamid Karimi, P. Ataei","doi":"10.1080/21645698.2022.2057160","DOIUrl":"https://doi.org/10.1080/21645698.2022.2057160","url":null,"abstract":"ABSTRACT This research aimed to study behavioral intention toward genetically modified crop (GMC) technology. The statistical population was composed of all staff experts of Jihad-e Agriculture Organization of Iran (N = 837). The sample size was 310 agricultural experts taken by simple randomization. The data collection tool was a questionnaire. Data were analyzed by structural equations modeling. The results provided enough evidence to confirm the negative and significant effect of ethical concerns on behavioral intention toward GMC technology and the positive and significant effect of attitude toward technology and social impact on behavioral intention. According to the results, it is necessary to hold training courses inside and outside the country, adopt bottom-up management, use experienced and prospective managers, involve experts in planning and development of GMCs to a greater extent, and share personal experiences in training courses to change people’ attitude.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"13 1","pages":"50 - 64"},"PeriodicalIF":3.9,"publicationDate":"2022-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49325783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sajid Fiaz, Xiukang Wang, Sher Aslam Khan, Sunny Ahmar, Mehmood Ali Noor, Aamir Riaz, Kazim Ali, Farhat Abbas, Freddy Mora-Poblete, Carlos R Figueroa, Badr Alharthi
{"title":"Novel plant breeding techniques to advance nitrogen use efficiency in rice: A review.","authors":"Sajid Fiaz, Xiukang Wang, Sher Aslam Khan, Sunny Ahmar, Mehmood Ali Noor, Aamir Riaz, Kazim Ali, Farhat Abbas, Freddy Mora-Poblete, Carlos R Figueroa, Badr Alharthi","doi":"10.1080/21645698.2021.1921545","DOIUrl":"https://doi.org/10.1080/21645698.2021.1921545","url":null,"abstract":"<p><p>Recently, there has been a remarkable increase in rice production owing to genetic improvement and increase in application of synthetic fertilizers. For sustainable agriculture, there is dire need to maintain a balance between profitability and input cost. To meet the steady growing demands of the farming community, researchers are utilizing all available resources to identify nutrient use efficient germplasm, but with very little success. Therefore, it is essential to understand the underlying genetic mechanism controlling nutrients efficiency, with the nitrogen use efficiency (NUE) being the most important trait. Information regarding genetic factors controlling nitrogen (N) transporters, assimilators, and remobilizers can help to identify candidate germplasms via high-throughput technologies. Large-scale field trials have provided morphological, physiological, and biochemical trait data for the detection of genomic regions controlling NUE. The functional aspects of these attributes are time-consuming, costly, labor-intensive, and less accurate. Therefore, the application of novel plant breeding techniques (NPBTs) with context to genome engineering has opened new avenues of research for crop improvement programs. Most recently, genome editing technologies (GETs) have undergone enormous development with various versions from Cas9, Cpf1, base, and prime editing. These GETs have been vigorously adapted in plant sciences for novel trait development to insure food quantity and quality. Base editing has been successfully applied to improve NUE in rice, demonstrating the potential of GETs to develop germplasms with improved resource use efficiency. NPBTs continue to face regulatory setbacks in some countries due to genome editing being categorized in the same category as genetically modified (GM) crops. Therefore, it is essential to involve all stakeholders in a detailed discussion on NPBTs and to formulate uniform policies tackling biosafety, social, ethical, and environmental concerns. In the current review, we have discussed the genetic mechanism of NUE and NPBTs for crop improvement programs with proof of concepts, transgenic and GET application for the development of NUE germplasms, and regulatory aspects of genome edited crops with future directions considering NUE.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"627-646"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1921545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39031227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Salman Mubarik, Xiukang Wang, Sultan Habibullah Khan, Aftab Ahmad, Zulqurnain Khan, Muhammad Waqas Amjid, Muhammad Khuram Razzaq, Zulfiqar Ali, Muhammad Tehseen Azhar
{"title":"Engineering broad-spectrum resistance to cotton leaf curl disease by CRISPR-Cas9 based multiplex editing in plants.","authors":"Muhammad Salman Mubarik, Xiukang Wang, Sultan Habibullah Khan, Aftab Ahmad, Zulqurnain Khan, Muhammad Waqas Amjid, Muhammad Khuram Razzaq, Zulfiqar Ali, Muhammad Tehseen Azhar","doi":"10.1080/21645698.2021.1938488","DOIUrl":"https://doi.org/10.1080/21645698.2021.1938488","url":null,"abstract":"<p><p>Advances in genome editing technologies have tremendous potential to address the limitations of classical resistance breeding. CRISPR-Cas9 based gene editing has been applied successfully in plants to tolerate virus infections. In this study, we successfully tested CRISPR-Cas9 system to counteract cotton leaf curl disease (CLCuD) caused by whitefly transmitted cotton leaf curl viruses (CLCuVs). We also analyzed the ability of CLCuV to escape the Cas9 endonuclease activity. Targeting overlapping genes of most prevalent CLCuVs with three gRNAs resulted in virus interference, as validated by low virus titer. Furthermore, multiplex CRISPR-Cas9 construct simultaneously targeting six genes of CLCuV, was found more effective to interfere with virus proliferation compared to targeting single region individually. Additionally, transgenic <i>N. benthamiana</i> plants expressing multiple gRNAs simultaneously showed enhanced tolerance against CLCuV infection when compared to wild-type plants. T7 Endonuclease-I (T7EI) assay, showing indels in the CLCuV genome, confirmed the occurrence of double strand breaks (DSBs) in DNA at target sequence induced by Cas9 endonuclease. We observed that targeting CLCuV genome at multiple sites simultaneously resulted in better interference, also with inefficient recovery of altered virus molecules. Next, we tested multiplex construct in cotton to interfere CLCuV infection. We found significant decrease in virus accumulation in cotton leaves co-infiltrated with multiplex cassette and virus compared to cotton leaves infiltrated with virus only. The results demonstrate future use of CRISPR-Cas9 system for engineering virus resistance in crops. Moreover, our results also advocate that resistance to mixed virus infections can be engineered using multiplex genome editing.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"647-658"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1938488","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39091673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brandon R McFadden, Joy N Rumble, Kathryn A Stofer, Kevin M Folta, Savanna Turner, Adam Pollack
{"title":"Gene editing isn't just about food: comments from U.S. focus groups.","authors":"Brandon R McFadden, Joy N Rumble, Kathryn A Stofer, Kevin M Folta, Savanna Turner, Adam Pollack","doi":"10.1080/21645698.2021.1919485","DOIUrl":"https://doi.org/10.1080/21645698.2021.1919485","url":null,"abstract":"<p><p>In the United States, adult public perception of genetic modification has been well documented in the domain of agriculture and food; however, recent international news on gene editing in medical applications may present new challenges for science communicators who seek to proactively share benefits of emerging gene editing technology. While research traditionally considers perceptions of agricultural and medical applications separately, gene editing may bridge the gap between the two domains. We find that when asked about thoughts regarding gene editing, adult focus groups discussed medical applications more frequently and extensively than agricultural applications. Although, when examining the length of discussion about specific topics, designer babies, cures for disease, and food were discussed at similar lengths. Understanding audiences' current perceptions of the technology is the first step in shaping strategic communication efforts to inform public opinion. A proper understanding of the benefits and risks of new technology is central to its application.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"616-626"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1919485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39000752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome editing techniques in plants: a comprehensive review and future prospects toward zero hunger.","authors":"Naglaa A Abdallah, Aladdin Hamwieh, Khaled Radwan, Nourhan Fouad, Channapatna Prakash","doi":"10.1080/21645698.2021.2021724","DOIUrl":"10.1080/21645698.2021.2021724","url":null,"abstract":"<p><p>Promoting sustainable agriculture and improving nutrition are the main united nation sustainable development goals by 2030. New technologies are required to achieve zero hunger, and genome editing technology is the most promising one. In the last decade, genome editing (GE) using the CRISPR/Cas system has attracted researchers as a safer and easy tool for genome editing in several living organisms. GE has revolutionized the field of agriculture by improving biotic and abiotic stresses and yield improvement. GE technologies were developed fast lately to avoid the obstacles that face GM crops. GE technology, depending on site directed nuclease (SDN), is divided into three categories according to the modification methods. Developing transgenic-free edited plants without introducing foreign DNA meet the acceptance and regulatory ratification of several countries. There are several ongoing efforts from different countries that are rapidly expanding to adopt the current technological innovations. This review summarizes the different GE technologies and their application as a way to help in ending hunger.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 2","pages":"601-615"},"PeriodicalIF":3.9,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39900643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ruth Mbabazi, Muffy Koch, Karim Maredia, Joseph Guenthner
{"title":"Crop Biotechnology and Product Stewardship.","authors":"Ruth Mbabazi, Muffy Koch, Karim Maredia, Joseph Guenthner","doi":"10.1080/21645698.2020.1822133","DOIUrl":"https://doi.org/10.1080/21645698.2020.1822133","url":null,"abstract":"<p><p>Agricultural biotechnology is enhancing agricultural productivity, food security, and livelihoods globally. Some developing countries have established functional biosafety regulatory systems and have commercialized genetically modified (GM) crops. Release of GM crops requires enhanced capacity for regulatory compliance and product stewardship to help ensure sustainable use of biotechnology products. We conducted a survey of 66 stakeholders, mostly from Africa and Asia, in two-week international agricultural biotechnology short courses. Respondents showed knowledge of biotechnology benefits and expressed potential barriers to commercialization. They identified 16 crops in the \"pipeline for commercialization.\" Stakeholders also shared ideas about how to build capacity for product stewardship. Product stewardship is a concept which requires each person in the product life cycle - innovators, scientists, and technology users, to share responsibility. This paper focuses on adoption of product stewardship for post-release management of GM crops which encompasses trait performance, resistance management, integrated pest management (IPM), good agricultural practices, high-quality seeds and planting material, intellectual property management, labeling, identity preservation, consumer acceptance, and effective marketing.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"106-114"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1822133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38508633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}