aBIOTECH最新文献_第3页

C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases C 端框移突变可产生具有发育缺陷的可行基因敲除突变体，这三种蛋白激酶至关重要

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-15 DOI: 10.1007/s42994-024-00165-5

Yun Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen, Kabin Xie

{"title":"C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases","authors":"Yun Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen, Kabin Xie","doi":"10.1007/s42994-024-00165-5","DOIUrl":"10.1007/s42994-024-00165-5","url":null,"abstract":"<div><p>Loss-of-function mutants are fundamental resources for gene function studies. However, it is difficult to generate viable and heritable knockout mutants for essential genes. Here, we show that targeted editing of the C-terminal sequence of the embryo lethal gene <i>MITOGEN-ACTIVATED PROTEIN KINASES 1</i> (<i>OsMPK1</i>) results in weak mutants. This C-terminal-edited osmpk1 mutants displayed severe developmental defects and altered disease resistance but generated tens of viable seeds that inherited the mutations. Using the same C-terminal editing approach, we also obtained viable mutants for a wall-associated protein kinase (Os07g0493200) and a leucine-rich repeat receptor-like protein kinase (Os01g0239700), while the null mutations of these genes were lethal. These data suggest that protein kinase activity could be reduced by introducing frameshift mutations adjacent to the C-terminus, which could generate valuable resources for gene function studies and tune protein kinase activity for signaling pathway engineering.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"219 - 224"},"PeriodicalIF":4.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00165-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis 玉米转录因子 ZmbHLH36 的异源表达可增强拟南芥的非生物胁迫耐受性

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00159-3

Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, Huawen Zou, Zhongyi Wu, Chun Zhang

{"title":"Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis","authors":"Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, Huawen Zou, Zhongyi Wu, Chun Zhang","doi":"10.1007/s42994-024-00159-3","DOIUrl":"10.1007/s42994-024-00159-3","url":null,"abstract":"<div><p>Basic helix-loop-helix (bHLH) transcription factors are widely distributed in eukaryotes, and in plants, they regulate many biological processes, such as cell differentiation, development, metabolism, and stress responses. Few studies have focused on the roles of bHLH transcription factors in regulating growth, development, and stress responses in maize (<i>Zea mays</i>), even though such information would greatly benefit maize breeding programs. In this study, we cloned the maize transcription factor gene <i>ZmbHLH36</i> (Gene ID: 100193615, GRMZM2G008691). ZmbHLH36 possesses conserved domains characteristic of the bHLH family. RT-qPCR analysis revealed that <i>ZmbHLH36</i> was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress conditions. Transgenic <i>Arabidopsis</i> (<i>Arabidopsis thaliana</i>) plants heterologously expressing <i>ZmbHLH36</i> had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L<sup>−1</sup> NaCl treatment as well as 0.2 mol L<sup>−1</sup> mannitol treatment. Phenotypic analysis of soil-grown plants under stress showed that transgenic <i>Arabidopsis</i> plants harboring <i>ZmbHLH36</i> exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic plants. Malondialdehyde contents were lower and peroxidase activity was higher in <i>ZmbHLH36</i>-expressing <i>Arabidopsis</i> plants than in the corresponding non-transgenic plants. ZmbHLH36 localized to the nucleus when expressed in maize protoplasts. This study provides a systematic analysis of the effects of ZmbHLH36 on root growth, development, and stress responses in transgenic <i>Arabidopsis</i>, laying a foundation for further analysis of its roles and molecular mechanisms in maize.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"339 - 350"},"PeriodicalIF":4.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00159-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140982769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for adaptation to high light in plants 植物适应强光的策略

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00164-6

Man Zhang, Yu Ming, Hong-Bin Wang, Hong-Lei Jin

{"title":"Strategies for adaptation to high light in plants","authors":"Man Zhang, Yu Ming, Hong-Bin Wang, Hong-Lei Jin","doi":"10.1007/s42994-024-00164-6","DOIUrl":"10.1007/s42994-024-00164-6","url":null,"abstract":"<div><p>Plants absorb light energy for photosynthesis via photosystem complexes in their chloroplasts. However, excess light can damage the photosystems and decrease photosynthetic output, thereby inhibiting plant growth and development. Plants have developed a series of light acclimation strategies that allow them to withstand high light. In the first line of defense against excess light, leaves and chloroplasts move away from the light and the plant accumulates compounds that filter and reflect the light. In the second line of defense, known as photoprotection, plants dissipate excess light energy through non-photochemical quenching, cyclic electron transport, photorespiration, and scavenging of excess reactive oxygen species. In the third line of defense, which occurs after photodamage, plants initiate a cycle of photosystem (mainly photosystem II) repair. In addition to being the site of photosynthesis, chloroplasts sense stress, especially light stress, and transduce the stress signal to the nucleus, where it modulates the expression of genes involved in the stress response. In this review, we discuss current progress in our understanding of the strategies and mechanisms employed by plants to withstand high light at the whole-plant, cellular, physiological, and molecular levels across the three lines of defense.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"381 - 393"},"PeriodicalIF":4.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00164-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140984916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploiting viral vectors to deliver genome editing reagents in plants 利用病毒载体为植物提供基因组编辑试剂

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-08 DOI: 10.1007/s42994-024-00147-7

Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong, Jianmin Wan

引用次数: 0

Decoding the microbiome for sustainable agriculture 解码微生物组，促进可持续农业

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-07 DOI: 10.1007/s42994-024-00162-8

Kai Sun, Wei Zhang, Xiaolin Wang, Chuan-Chao Dai

{"title":"Decoding the microbiome for sustainable agriculture","authors":"Kai Sun, Wei Zhang, Xiaolin Wang, Chuan-Chao Dai","doi":"10.1007/s42994-024-00162-8","DOIUrl":"10.1007/s42994-024-00162-8","url":null,"abstract":"<div><p>Root-associated microbiota profoundly affect crop health and productivity. Plants can selectively recruit beneficial microbes from the soil and actively balance microbe-triggered plant-growth promotion and stress tolerance enhancement. The cost associated with this is the root-mediated support of a certain number of specific microbes under nutrient limitation. Thus, it is important to consider the dynamic changes in microbial quantity when it comes to nutrient condition-induced root microbiome reassembly. Quantitative microbiome profiling (QMP) has recently emerged as a means to estimate the specific microbial load variation of a root microbiome (instead of the traditional approach quantifying relative microbial abundances) and data from the QMP approach can be more closely correlated with plant development and/or function. However, due to a lack of detailed-QMP data, how soil nutrient conditions affect quantitative changes in microbial assembly of the root-associated microbiome remains poorly understood. A recent study quantified the dynamics of the soybean root microbiome, under unbalanced fertilization, using QMP and provided data on the use of specific synthetic communities (SynComs) for sustaining crop productivity. In this editorial, we explore potential opportunities for utilizing QMP to decode the microbiome for sustainable agriculture.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"408 - 412"},"PeriodicalIF":4.6,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Publisher Correction: Removal of the C4-domain preserves the drought tolerance enhanced by CsMYB4a and eliminates the negative impact of this transcription factor on plant growth 错误：出版者更正：移除 C4 域可保留 CsMYB4a 增强的耐旱性，并消除该转录因子对植物生长的负面影响。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-06 DOI: 10.1007/s42994-024-00163-7

Mingzhuo Li, Guoliang Ma, Xiu Li, Lili Guo, Yanzhi Li, Yajun Liu, Wenzhao Wang, Xiaolan Jiang, De-Yu Xie, Liping Gao, Tao Xia

引用次数: 0

Correction: Current overview on the genetic basis of key genes involved in soybean domestication 更正：大豆驯化关键基因的遗传基础概述。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-04-30 DOI: 10.1007/s42994-024-00161-9

Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu

引用次数: 0

Efficient and precise genomic deletion in rice using enhanced prime editing 利用增强的质粒编辑技术高效、精确地删除水稻基因组。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-04-29 DOI: 10.1007/s42994-024-00153-9

Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao, Jinxiao Yang

{"title":"Efficient and precise genomic deletion in rice using enhanced prime editing","authors":"Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao, Jinxiao Yang","doi":"10.1007/s42994-024-00153-9","DOIUrl":"10.1007/s42994-024-00153-9","url":null,"abstract":"<div><p>Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research and enhancing agricultural traits. In this study, we tested the PRIME-Del (PDel) strategy using a pair of prime editing guide RNAs (pegRNAs) that targeted opposite DNA strands and achieved an average deletion efficiency of 55.8% for 60 bp fragment deletions at six endogenous targets. Moreover, as high as 84.2% precise deletion efficiency was obtained for a 2000 bp deletion at the <i>OsGS1</i> site in transgenic rice plants. To add the bases that were unintentionally deleted between the two nicking sequences, we used the PDel/Syn strategy, which introduced multiple synonymous base mutations in the region that had to be patched in the RT template. The PDel/Syn strategy achieved an average of 58.1% deletion efficiency at six endogenous targets, which was higher than the PDel strategy. The strategies presented in this study contribute to achieving more accurate and flexible deletions in transgenic rice plants.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"214 - 218"},"PeriodicalIF":4.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a CRISPR/FrCas9 system for core promoter editing in rice 开发用于水稻核心启动子编辑的 CRISPR/FrCas9 系统

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-04-22 DOI: 10.1007/s42994-024-00157-5

Hui Wang, Jian Ding, Jingyan Zhu, Xiaoshuang Liu, Rongfang Xu, Ruiying Qin, Dongfang Gu, Min Li, Pengcheng Wei, Juan Li

{"title":"Developing a CRISPR/FrCas9 system for core promoter editing in rice","authors":"Hui Wang, Jian Ding, Jingyan Zhu, Xiaoshuang Liu, Rongfang Xu, Ruiying Qin, Dongfang Gu, Min Li, Pengcheng Wei, Juan Li","doi":"10.1007/s42994-024-00157-5","DOIUrl":"10.1007/s42994-024-00157-5","url":null,"abstract":"<div><p>Small mutations in the core promoter region of a gene may result in substantial changes in expression strengths. However, targeting TA-rich sequences of core promoters may pose a challenge for Cas9 variants such as SpCas9 and other G-rich PAM-compatible Cas9s. In this study, we engineered a unique FrCas9 system derived from <i>Faecalibaculum rodentium</i> for plant genome editing. Our findings indicate that this system is efficient in rice when the TATA sequence is used as a PAM. In addition, FrCas9 demonstrated activity against all 16 possible NNTA PAMs, achieving an efficiency of up to 35.3% in calli and generating homozygous or biallelic mutations in 31.3% of the T<sub>0</sub> transgenic plants. A proof-of-concept experiment to examine editing of the rice <i>WX</i> core promoter confirmed that FrCas9-induced mutations could modify gene expression and amylose content. Multiplex mutations and deletions were produced by bidirectional editing, mediated by FrCas9, using a single palindromic TATA sequence as a PAM. Moreover, we developed FrCas9-derived base editors capable of programmable conversion between A·T and G·C pairs in plants. This study highlights a versatile FrCas9 toolset for plant core promoter editing, offering great potential for the fine-tuning of gene expression and creating of new germplasms.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"189 - 195"},"PeriodicalIF":4.6,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00157-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140676941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural GmACO1 allelic variations confer drought tolerance and influence nodule formation in soybean 天然 GmACO1 等位基因变异赋予大豆耐旱性并影响其结核形成

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-04-18 DOI: 10.1007/s42994-024-00160-w

Zhifang Zhang, Junkui Ma, Xia Yang, Shan Liang, Yucheng Liu, Yaqin Yuan, Qianjin Liang, Yanting Shen, Guoan Zhou, Min Zhang, Zhixi Tian, Shulin Liu

引用次数: 0