OsbHLH062 regulates iron homeostasis by inhibiting iron deficiency responses in rice

IF 5 4区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

aBIOTECH Pub Date : 2025-03-03 DOI:10.1007/s42994-025-00203-w

Wujian Wang, Fengyu He, Hui Zhang, Yue Yang, Xiaojuan Wang, Yue Fu, Huixia Shou, Luqing Zheng

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Abstract

Iron (Fe) homeostasis in plant cells is crucial for crop productivity and quality. An intricate transcriptional network involving numerous basic Helix-Loop-Helix (bHLH) transcription factors has been proposed to control Fe homeostasis. In the present study, we characterized rice (Oryza sativa) OsbHLH062, a member of the IVb subgroup of the bHLH family, demonstrating that it negatively regulates Fe-deficiency responses. OsbHLH062 represses transcription by recruiting TOPLESS/TOPLESS-RELATED co-repressors (TPL/TPRs) through its ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif. Under Fe deficiency, the expression of OsbHLH062 is upregulated in roots and downregulated in shoots. Overexpression of OsbHLH062 leads to decreased Fe accumulation in the shoot. Furthermore, OsbHLH062 interacts with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 (OsPRI1) and inhibits its transactivation activity, thereby negatively regulating the expression of many Fe homeostasis-related genes. These results indicate an important role for OsbHLH062 in regulating Fe homeostasis by negatively regulating Fe deficiency responses in rice. This knowledge will aid in the design of Fe-biofortified rice plants that can help to address the global issue of Fe deficiency.

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OsbHLH062通过抑制水稻缺铁反应调节铁稳态。

植物细胞中的铁稳态对作物产量和品质至关重要。一个复杂的转录网络涉及许多基本的螺旋-环-螺旋（bHLH）转录因子被提出控制铁稳态。在本研究中，我们对bHLH家族IVb亚群中的成员水稻（Oryza sativa） OsbHLH062进行了表征，证明它负调控铁缺乏反应。OsbHLH062通过其乙烯响应元件结合因子相关两亲性抑制（EAR）基序招募toppless / toppless相关共抑制因子（TPL/TPRs）来抑制转录。缺铁条件下，OsbHLH062在根中表达上调，在茎中表达下调。OsbHLH062过表达导致茎部铁积累减少。此外，OsbHLH062与铁稳态正调节因子1 （POSITIVE REGULATOR OF IRON HOMEOSTASIS 1, OsPRI1）相互作用，抑制其交互激活活性，从而负向调节许多铁稳态相关基因的表达。这些结果表明，OsbHLH062通过负调控水稻缺铁反应，在铁稳态调节中起重要作用。这些知识将有助于设计铁生物强化水稻，从而有助于解决全球缺铁问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

aBIOTECH

CiteScore

7.70

自引率

2.80%

发文量