aBIOTECH最新文献

{"title":"Mutation of <i>ERECTA</i> homologous genes confers ideal plant architecture in <i>Brassica napus</i>.","authors":"Hui Zhang, Tao Wang, Jianwei Gu, Dengfeng Hong","doi":"10.1007/s42994-025-00217-4","DOIUrl":"https://doi.org/10.1007/s42994-025-00217-4","url":null,"abstract":"<p><p>Varieties with a semi-dwarf compact plant architecture may increase yield per unit area in rapeseed (<i>Brassica napus</i>) by allowing high-density cultivation and mechanical harvesting while conferring lodging resistance. Mutation of <i>ERECTA</i> (<i>ER</i>), which encodes a receptor-like protein kinase, generates a compact and upright plant architecture in <i>Arabidopsis thaliana</i>; however, there have been no reports on the roles of the ER family (ERf) in <i>B. napus</i>. In this study, we used the CRISPR/Cas9 system to generate mutants in each of the two homoeologs of <i>B. napus ERf</i> members <i>BnaER</i> and <i>ER-Like 1</i> (<i>BnaERL1</i>), and in the single <i>BnaERL2</i> gene, resulting in the homozygous mutants <i>BnaA09.er/BnaC08.er</i>, <i>BnaA06.erl1</i>/<i>BnaC03.erl1</i>, and <i>BnaA10.erl2</i>. Under greenhouse conditions, <i>BnaA09.er</i>/<i>BnaC08.er</i> plants were shorter than the wild type, with a compact inflorescence and shorter siliques. In addition, <i>BnaA09.er</i>/<i>BnaC08.er</i> plants produced significantly more branches and total siliques than the wild type, with no significant changes in the number of ovules per silique or thousand-seed weight. Under field conditions, the <i>BnaA09.er/BnaC08.er</i> mutant plant showed a phenotype comparable to that under greenhouse conditions, but with a notable drop in thousand-seed weight. These results indicate that the <i>BnaA09.er/BnaC08.er</i> mutant offers a valuable germplasm resource for breeding rapeseed with ideal plant architecture.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00217-4.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"249-262"},"PeriodicalIF":4.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Observe natural selection by evolutionary experiments in crops.","authors":"Tian Wu, Shifeng Cheng","doi":"10.1007/s42994-025-00215-6","DOIUrl":"10.1007/s42994-025-00215-6","url":null,"abstract":"<p><p>Evolutionary experiments provide a unique lens through which to observe the impacts of natural selection on crop evolution, domestication, and adaptation through empirical evidence. Enabled by modern technologies-such as the development of large-scale, structured evolving populations, high-throughput phenotyping, and genomics-driven genetics studies-the transition from theoretical evolutionary biology to practical application is now possible for staple crops. The century-long Barley Composite Cross II (CCII) competition experiment has offered invaluable insights into understanding the genomic and phenotypic basis of natural and artificial selection driven by environmental adaptation during crop evolution and domestication. These experiments enable scientists to measure evolutionary dynamics, in real time, of genetic diversity, adaptation of fitness-associated traits, and the trade-offs inherent in selective processes. Beyond advancing our understanding of evolutionary biology and agricultural practices, these studies provide critical insights into addressing global challenges, from ensuring food security to fostering resilience in human societies.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"381-387"},"PeriodicalIF":4.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agricultural biotechnology in China: product development, commercialization, and perspectives.","authors":"Jingang Liang, Yu Sun, Yanchao Yang, Zeyu Wang, Han Wu, Taotao Gu, Ruifu Zhang, Xinli Sun, Bin Yao, Tao Tu, Xiaoqing Liu, Huiying Luo, Guangzhi Tong, Yue Jiao, Kui Li, Jie Zhang, Kongming Wu","doi":"10.1007/s42994-025-00209-4","DOIUrl":"10.1007/s42994-025-00209-4","url":null,"abstract":"<p><p>Meeting the increasing demand for food and industrial products by the growing global population requires targeted efforts to improve crops, livestock, and microorganisms. Modern biotechnology, particularly genetic modification (GM) and genome-editing (GE) technologies, is crucial for food security and environmental sustainability. China, which is at the forefront of global biotechnological innovation and the rapid advancements in GM and GE technologies, has prioritized this field by implementing strategic programs such as the National High-tech Research & Development Program in 1986, the National Genetically Modified Organism New Variety Breeding Program in 2008, and the Biological Breeding-National Science and Technology Major Project in 2022. Many biotechnological products have been widely commercialized in China, including biofertilizers, animal feed, animal vaccines, pesticides, and GM crops such as cotton (<i>Gossypium hirsutum</i>), maize (<i>Zea mays</i>), and soybean (<i>Glycine max</i>). In this review, we summarize progress on the research and utilization of GM and GE organisms in China over the past 3 decades and provide perspectives on their further development. This review thus aims to promote worldwide academic exchange and contribute to the further development and commercial success of agricultural biotechnology.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"284-310"},"PeriodicalIF":4.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soyasaponin β-glucosidase confers soybean resistance to pod borer (<i>Leguminivora glycinivorella</i>).","authors":"Chengyong Feng, Xindan Xu, Jia Yuan, Mingyu Yang, Fanli Meng, Guodong Wang","doi":"10.1007/s42994-025-00214-7","DOIUrl":"10.1007/s42994-025-00214-7","url":null,"abstract":"<p><p>Plant specialized metabolites are commonly stored in glycosylated forms within plant cells, with their homeostasis regulated by glycosyltransferases and β-glucosidases (BGLUs, also known as β-glucoside hydrolases (E.C.3.2.1.21)). Soyasaponins, the predominant triterpenoid compounds (C30) in soybean seeds, contain two sugar moieties attached at the C3 and C22 positions. While glycosyltransferases involved in soyasaponin biosynthesis have been well characterized, the role of BGLUs in soyasaponin homeostasis remains unclear. In this study, we identified <i>GmSSBG1</i> (<i>Soyasaponin β-glucosidase1</i>; <i>Glyma.07G258700</i>) as a candidate gene potentially involved in soyasaponin homeostasis through gene to gene co-expression analysis. Biochemical assays demonstrated that GmSSBG1 specifically hydrolyzes arabinose residues at the C22 position of A0- and B0-series soyasaponins. Loss-of-function mutations in <i>GmSSBG1</i> led to a significant accumulation of A0- and B0-series soyasaponins in mutant seeds, which correlated with a pronounced decrease in resistance to the soybean pod borer (<i>Leguminivora glycinivorella</i>). Our findings provide critical insights into the regulatory mechanisms underlying soyasaponin homeostasis and lay a theoretical foundation for molecular breeding strategies aimed at developing soybean lines with enhanced resistance to soybean pod borer, even to other insect pests.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00214-7.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"160-173"},"PeriodicalIF":4.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation in <i>Medicago truncatula</i>.","authors":"Fanghao Sun, Fugui Zhu, Shasha Ran, Qinyi Ye, Tao Wang, Jiangli Dong","doi":"10.1007/s42994-025-00210-x","DOIUrl":"10.1007/s42994-025-00210-x","url":null,"abstract":"<p><p>Heterotrimeric G proteins are crucial transducers of signaling from receptors, participating in growth and development, as well as in responses to biotic and abiotic stimuli. However, little is known about their roles in regulating various yield-related traits in legumes. In this study, we systematically analyzed the functions of two G-protein-encoding genes, <i>MtGα1</i> and <i>MtGβ1</i>, along with <i>Regulator of G-protein Signaling1</i> (<i>MtRGS1</i>), in <i>Medicago truncatula</i>. All three genes were ubiquitously expressed in roots, stems, leaves, nodules, flowers, and pods. We generated the knockout mutants <i>Mtgα1</i>, <i>Mtgβ1</i>, and <i>Mtrgs1</i> using CRISPR/Cas9 and assessed their growth and development. <i>MtGα1</i> knockout resulted in slightly shorter plants with smaller pods and shorter spines, but larger seeds, without affecting overall biomass or other traits. <i>MtGβ1</i> knockout led to dwarfism, weak root development, a severe drop in biomass production, smaller legume pods with shorter spines, and smaller seeds. However, the <i>Mtrgs1</i> mutants were largely similar to wild-type plants, with few significant defects in growth and development. We also investigated the symbiotic nodulation-related phenotypes of these mutants, discovering that <i>Mtgβ1</i> mutants produce lighter nodules, whereas <i>Mtgα1</i> and <i>Mtrgs1</i> mutants have normal nodulation phenotypes similar to those of wild-type plants. These observations suggest that MtGβ1 positively regulates nodulation, although the detailed mechanisms by which G proteins regulate symbiotic nitrogen fixation in <i>M. truncatula</i> remain to be explored. This work provides potentially valuable genetic resources for further functional analysis and elucidation of the molecular mechanisms of G proteins in this model legume.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00210-x.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"141-159"},"PeriodicalIF":4.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous knockout of multiple <i>eukaryotic translation initiation factor 4E</i> genes confers durable and broad-spectrum resistance to potyviruses in tobacco.","authors":"Yong Liu, Shuo Wang, Danyang Zhao, Chenglu Zhao, Haiqin Yu, Jianmin Zeng, Zhijun Tong, Cheng Yuan, Zhenghe Li, Changjun Huang","doi":"10.1007/s42994-025-00216-5","DOIUrl":"10.1007/s42994-025-00216-5","url":null,"abstract":"<p><p>Recessive resistance mediated by mutations in the eukaryotic translation initiation factor 4E (eIF4E), has proven effective against diverse potyviruses and is extensively utilized in breeding programs. However, the rise of resistance-breaking (RB) strains and emerging potyviral species necessitates the development of more durable and broad-spectrum resistance strategies. In this study, our field survey in Yunnan, China, identified potato virus Y (PVY) RB isolates, as well as the prevalence of tobacco vein banding mosaic virus (TVBMV) and chilli veinal mottle virus (ChiVMV), in tobacco carrying the recessive <i>va</i> locus, which lacks the <i>eIF4E1-S</i> susceptibility gene, due to a chromosomal deletion. Protein interaction and viral infection assays demonstrated that both eIF4E1-S and eIFiso4E-T are used by PVY RB as susceptibility factors for infection, with the combined inactivation of these genes confering durable resistance. Similarly, the knockout of <i>eIFiso4E-S,</i> in the <i>va</i> genetic background, provided effective resistance to TVBMV and reduced susceptibility to ChiVMV. Notably, pyramiding mutations in <i>eIFiso4E-S</i> and <i>eIFiso4E-T</i>, in <i>va</i> tobacco, generated plants exhibiting robust, broad-spectrum resistance, to all three viruses, without compromising plant development. These findings underscore the potential of stacking eIF4E mutations to engineer durable, broad-spectrum resistance to potyviruses in tobacco, offering a promising strategy for crop improvement.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00216-5.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"232-248"},"PeriodicalIF":4.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SuperDecode: A versatile toolkit for mutation analysis in genome editing.","authors":"Guoying Hua, Chenfei He, Erwei Zuo","doi":"10.1007/s42994-025-00213-8","DOIUrl":"https://doi.org/10.1007/s42994-025-00213-8","url":null,"abstract":"<p><p>The CRISPR-Cas system has revolutionized modern life sciences, enabling groundbreaking applications ranging from functional genomics to therapeutic development. Despite its transformative potential, significant technical limitations persist in current computational tools for quantifying editing efficiency - particularly concerning data processing capabilities, analytical throughput, and operational flexibility. This research presents SuperDecode, a novel computational framework designed to address these methodological constraints. The SuperDecode offers key advantages, including local processing capabilities, large-size sequencing files, batch-processing, and diversified operational functions.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"377-380"},"PeriodicalIF":4.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeding and molecular characterization of a new salt-tolerant wheat variety.","authors":"Wanqing Bai, Ziyi Yang, Shuxian Huang, Anqi Li, Liming Wang, Yunwei Zhang, Jiaqiang Sun","doi":"10.1007/s42994-025-00211-w","DOIUrl":"https://doi.org/10.1007/s42994-025-00211-w","url":null,"abstract":"<p><p>Soil salinization is a severely detrimental environmental problem that affects the seed germination, growth and yield of wheat. To excavate salt-tolerant genes and breed salt-tolerant wheat varieties are of great significance for ensuring global food security. In this study, we have successfully developed a novel salt-tolerant wheat cultivar, KD808, which is shown to have remarkable salt tolerance through multiple phenotypic analyses. RNA-seq coupled with RT-qPCR analyses reveal that the expression of <i>TaSGR-5B</i> is up-regulated by salt stress treatment in the salt-sensitive wheat varieties such as KN199 and Fielder, whereas the salt-induction of <i>TaSGR-5B</i> is abolished in our salt-tolerant variety KD808. More importantly, we found that the loss-of-function <i>Tasgr-aabbdd</i> mutants exhibit significantly salt-tolerant phenotypes without penalties in major agronomic traits. This study not only provides valuable insights into the molecular mechanisms of salt tolerance in wheat but also offers substantial potential for improving wheat cultivation in saline-alkali soils, thereby contributing to sustainable agricultural production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-025-00211-w.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"278-283"},"PeriodicalIF":4.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Mildew resistance locus O</i> (<i>MLO</i>)-edited petunia shows reduced seed germination, seed set, seed vigor, and growth.","authors":"Aung Htay Naing, Jova Riza Campol, Hay Mon Aung, Hyunhee Kang, Su Bin Cho, Yun-Jae Ahn, Junping Xu, Mi Young Chung, Chang Kil Kim","doi":"10.1007/s42994-024-00187-z","DOIUrl":"https://doi.org/10.1007/s42994-024-00187-z","url":null,"abstract":"<p><p>In this study, we evaluated the impact of editing the powdery mildew-susceptible gene <i>MLO1</i> in <i>Petunia hybrida</i> cv. 'Mirage Rose' using CRISPR/Cas9. We evaluated plant growth, seed physiology, pollen viability and germination, and gibberellin (GA)-associated gene expression. The <i>mlo1</i> mutants exhibited delayed seed germination, reduced plant growth, delayed flowering, and lower seed set and weight compared to wild type (WT). While pollen viability and germination were not significantly affected in the <i>mlo1-14</i> mutant, both were notably reduced in the <i>mlo1-25</i> mutant. Expression analysis revealed significantly higher levels of GA biosynthesis and receptor genes in the mutants, suggesting alterations in the GA signaling pathways. Application of exogenous GA₃ effectively mitigated growth inhibition in the mutants, aligning their growth parameters with those of WT. Our findings underscore the complex role of <i>MLO1</i> in regulating plant growth and development, likely through modulation of the GA signaling pathways. This study highlights the trade-offs associated with <i>MLO1</i> editing for powdery mildew resistance, including impacts on plant growth and reproductive success, and demonstrates the potential of GA₃ application in counteracting these effects. Collectively, this study suggests that the growth penalty and impaired seed physiology caused by the <i>mlo1</i> mutation must be considered when attempting to edit <i>MLO1</i> for powdery mildew toleration in ornamental plants, as these drawbacks can severely impact the plants' utility in the floricultural industry.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-024-00187-z.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"263-273"},"PeriodicalIF":4.6,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant graph-based pangenomics: techniques, applications, and challenges.","authors":"Ze-Zhen Du, Jia-Bao He, Wen-Biao Jiao","doi":"10.1007/s42994-025-00206-7","DOIUrl":"10.1007/s42994-025-00206-7","url":null,"abstract":"<p><p>Innovations in DNA sequencing technologies have greatly boosted population-level genomic studies in plants, facilitating the identification of key genetic variations for investigating population diversity and accelerating the molecular breeding of crops. Conventional methods for genomic analysis typically rely on small variants, such as SNPs and indels, and use single linear reference genomes, which introduces biases and reduces performance in highly divergent genomic regions. By integrating the population level of sequences, pangenomes, particularly graph pangenomes, offer a promising solution to these challenges. To date, numerous algorithms have been developed for constructing pangenome graphs, aligning reads to these graphs, and performing variant genotyping based on these graphs. As demonstrated in various plant pangenomic studies, these advancements allow for the detection of previously hidden variants, especially structural variants, thereby enhancing applications such as genetic mapping of agronomically important genes. However, noteworthy challenges remain to be overcome in applying pangenome graph approaches to plants. Addressing these issues will require the development of more sophisticated algorithms tailored specifically to plants. Such improvements will contribute to the scalability of this approach, facilitating the production of super-pangenomes, in which hundreds or even thousands of de novo-assembled genomes from one species or genus can be integrated. This, in turn, will promote broader pan-omic studies, further advancing our understanding of genetic diversity and driving innovations in crop breeding.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"6 2","pages":"361-376"},"PeriodicalIF":4.6,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}