IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-07-19 DOI: 10.1007/s42994-024-00174-4

P. Zhou, Xiujin Liu, Yuqing Liang, Yan Zhang, Xiaoshuang Li, Daoyuan Zhang

引用次数: 0

Inference and prioritization of tissue-specific regulons in Arabidopsis and Oryza 拟南芥和旱生植物组织特异性调控子的推断和优先排序

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-07-16 DOI: 10.1007/s42994-024-00176-2

Honggang Dai, Yaxin Fan, Yichao Mei, Ling-Ling Chen, Junxiang Gao

引用次数: 0

The acyl-CoA-binding protein VdAcb1 is essential for carbon starvation response and contributes to virulence in Verticillium dahliae 酰基-CoA 结合蛋白 VdAcb1 对于大丽轮枝菌的碳饥饿反应至关重要，并有助于提高其毒力

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-07-13 DOI: 10.1007/s42994-024-00175-3

Zhuang Jing, Ya-Duo Zhang, Wei-xia Sun, Juan Zong, Jun-Jiao Li, Xiao-Feng Dai, S. Klosterman, Jiemin Chen, Li Tian, K. Subbarao, Dan-Dan Zhang

引用次数: 0

Simultaneous genetic transformation and genome editing of mixed lines in soybean (Glycine max) and maize (Zea mays). 大豆（Glycine max）和玉米（Zea mays）混合品系的同步遗传转化和基因组编辑。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-06-18 eCollection Date: 2024-06-01 DOI: 10.1007/s42994-024-00173-5

Michelle Valentine, David Butruille, Frederic Achard, Steven Beach, Brent Brower-Toland, Edward Cargill, Megan Hassebrock, Jennifer Rinehart, Thomas Ream, Yurong Chen

{"title":"Simultaneous genetic transformation and genome editing of mixed lines in soybean (Glycine max) and maize (Zea mays).","authors":"Michelle Valentine, David Butruille, Frederic Achard, Steven Beach, Brent Brower-Toland, Edward Cargill, Megan Hassebrock, Jennifer Rinehart, Thomas Ream, Yurong Chen","doi":"10.1007/s42994-024-00173-5","DOIUrl":"10.1007/s42994-024-00173-5","url":null,"abstract":"Robust genome editing technologies are becoming part of the crop breeding toolbox. Currently, genome editing is usually conducted either at a single locus, or multiple loci, in a variety at one time. Massively parallel genomics platforms, multifaceted genome editing capabilities, and flexible transformation systems enable targeted variation at nearly any locus, across the spectrum of genotypes within a species. We demonstrate here the simultaneous transformation and editing of many genotypes, by targeting mixed seed embryo explants with genome editing machinery, followed by re-identification through genotyping after plant regeneration. Transformation and Editing of Mixed Lines (TREDMIL) produced transformed individuals representing 101 of 104 (97%) mixed elite genotypes in soybean; and 22 of 40 (55%) and 9 of 36 (25%) mixed maize female and male elite inbred genotypes, respectively. Characterization of edited genotypes for the regenerated individuals identified over 800 distinct edits at the Determinate1 (Dt1) locus in samples from 101 soybean genotypes and 95 distinct Brown midrib3 (Bm3) edits in samples from 17 maize genotypes. These results illustrate how TREDMIL can help accelerate the development and deployment of customized crop varieties for future precision breeding.Supplementary information: The online version contains supplementary material available at 10.1007/s42994-024-00173-5.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome editing in plants using the TnpB transposase system 利用 TnpB 转座酶系统进行植物基因组编辑

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2024-06-08 DOI: 10.1007/s42994-024-00172-6

Qi Li, Yongqiang Wang, Zhuoting Hou, Hang Zong, Xuping Wang, Yong E. Zhang, Haoyi Wang, Haitao Chen, Wen Wang, Kang Duan

引用次数: 0

Efficient genome editing in rice with miniature Cas12f variants. 利用微型 Cas12f 变体对水稻进行高效基因组编辑。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-05-28 eCollection Date: 2024-06-01 DOI: 10.1007/s42994-024-00168-2

Zhengyan Ye, Yuanyan Zhang, Shiqi He, Shaokang Li, Longjiong Luo, Yanbiao Zhou, Junjie Tan, Jianmin Wan

{"title":"Efficient genome editing in rice with miniature Cas12f variants.","authors":"Zhengyan Ye, Yuanyan Zhang, Shiqi He, Shaokang Li, Longjiong Luo, Yanbiao Zhou, Junjie Tan, Jianmin Wan","doi":"10.1007/s42994-024-00168-2","DOIUrl":"10.1007/s42994-024-00168-2","url":null,"abstract":"Genome editing, particularly using the CRISPR/Cas system, has revolutionized biological research and crop improvement. Despite the widespread use of CRISPR/Cas9, it faces limitations such as PAM sequence requirements and challenges in delivering its large protein into plant cells. The hypercompact Cas12f, derived from Acidibacillus sulfuroxidans (AsCas12f), stands out due to its small size of only 422 amino acids and its preference for a T-rich motif, presenting advantageous features over SpCas9. However, its editing efficiency is extremely low in plants. Recent studies have generated two AsCas12f variants, AsCas12f-YHAM and AsCas12f-HKRA, demonstrating higher editing efficiencies in mammalian cells, yet their performance in plants remains unexplored. In this study, through a systematic investigation of genome cleavage activity in rice, we unveiled a substantial enhancement in editing efficiency for both AsCas12f variants, particularly for AsCas12f-HKRA, which achieved an editing efficiency of up to 53%. Furthermore, our analysis revealed that AsCas12f predominantly induces deletion in the target DNA, displaying a unique deletion pattern primarily concentrated at positions 12, 13, 23, and 24, resulting in deletion size mainly of 10 and 11 bp, suggesting significant potential for targeted DNA deletion using AsCas12f. These findings expand the toolbox for efficient genome editing in plants, offering promising prospects for precise genetic modifications in agriculture.Supplementary information: The online version contains supplementary material available at 10.1007/s42994-024-00168-2.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isochorismate synthase is required for phylloquinone, but not salicylic acid biosynthesis in rice 水稻的植物喹啉酮生物合成需要异橙皮苷酸合成酶，而水杨酸生物合成不需要异橙皮苷酸合成酶

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2024-05-24 DOI: 10.1007/s42994-024-00166-4

Zengqian Wang, Guiqing Yang, Dandan Zhang, Guangxin Li, Jin-Long Qiu, Jie Wu

引用次数: 0

Genome editing toward biofortified soybean with minimal trade-off between low phytic acid and yield 通过基因组编辑实现生物强化大豆，在低植酸和产量之间实现最小权衡

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2024-05-23 DOI: 10.1007/s42994-024-00158-4

Wenxin Lin, Mengyan Bai, Chunyan Peng, Huaqin Kuang, Fanjiang Kong, Yuefeng Guan

引用次数: 0

Early surveillance of rice bakanae disease using deep learning and hyperspectral imaging 利用深度学习和高光谱成像对水稻包虫病进行早期监测