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C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases C 端框移突变可产生具有发育缺陷的可行基因敲除突变体，这三种蛋白激酶至关重要
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-15 DOI: 10.1007/s42994-024-00165-5
Yun-mu Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen, Kabin Xie
{"title":"C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases","authors":"Yun-mu Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen, Kabin Xie","doi":"10.1007/s42994-024-00165-5","DOIUrl":"https://doi.org/10.1007/s42994-024-00165-5","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis 玉米转录因子 ZmbHLH36 的异源表达可增强拟南芥的非生物胁迫耐受性
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00159-3
Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, H. Zou, Zhongyi Wu, Chun Zhang
{"title":"Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis","authors":"Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, H. Zou, Zhongyi Wu, Chun Zhang","doi":"10.1007/s42994-024-00159-3","DOIUrl":"https://doi.org/10.1007/s42994-024-00159-3","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140982769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Strategies for adaptation to high light in plants 植物适应强光的策略
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00164-6
Man Zhang, Yu Ming, Hong-Bin Wang, Hong-Lei Jin
{"title":"Strategies for adaptation to high light in plants","authors":"Man Zhang, Yu Ming, Hong-Bin Wang, Hong-Lei Jin","doi":"10.1007/s42994-024-00164-6","DOIUrl":"https://doi.org/10.1007/s42994-024-00164-6","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140984916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploiting viral vectors to deliver genome editing reagents in plants 利用病毒载体为植物提供基因组编辑试剂
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-08 DOI: 10.1007/s42994-024-00147-7
Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong, Jianmin Wan
{"title":"Exploiting viral vectors to deliver genome editing reagents in plants","authors":"Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong, Jianmin Wan","doi":"10.1007/s42994-024-00147-7","DOIUrl":"https://doi.org/10.1007/s42994-024-00147-7","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141001110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Decoding the microbiome for sustainable agriculture 解码微生物组，促进可持续农业
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-07 DOI: 10.1007/s42994-024-00162-8
Kai Sun, Wei Zhang, Xiaolin Wang, Chuan-Chao Dai
{"title":"Decoding the microbiome for sustainable agriculture","authors":"Kai Sun, Wei Zhang, Xiaolin Wang, Chuan-Chao Dai","doi":"10.1007/s42994-024-00162-8","DOIUrl":"https://doi.org/10.1007/s42994-024-00162-8","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Current overview on the genetic basis of key genes involved in soybean domestication. 更正：大豆驯化关键基因的遗传基础概述。
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-30 eCollection Date: 2024-06-01 DOI: 10.1007/s42994-024-00161-9
Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu
{"title":"Correction: Current overview on the genetic basis of key genes involved in soybean domestication.","authors":"Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu","doi":"10.1007/s42994-024-00161-9","DOIUrl":"https://doi.org/10.1007/s42994-024-00161-9","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1007/s42994-022-00074-5.].</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient and precise genomic deletion in rice using enhanced prime editing. 利用增强的质粒编辑技术高效、精确地删除水稻基因组。
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-29 eCollection Date: 2024-06-01 DOI: 10.1007/s42994-024-00153-9
Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao, Jinxiao Yang
{"title":"Efficient and precise genomic deletion in rice using enhanced prime editing.","authors":"Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao, Jinxiao Yang","doi":"10.1007/s42994-024-00153-9","DOIUrl":"10.1007/s42994-024-00153-9","url":null,"abstract":"<p><p>Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research and enhancing agricultural traits. In this study, we tested the PRIME-Del (PDel) strategy using a pair of prime editing guide RNAs (pegRNAs) that targeted opposite DNA strands and achieved an average deletion efficiency of 55.8% for 60 bp fragment deletions at six endogenous targets. Moreover, as high as 84.2% precise deletion efficiency was obtained for a 2000 bp deletion at the <i>OsGS1</i> site in transgenic rice plants. To add the bases that were unintentionally deleted between the two nicking sequences, we used the PDel/Syn strategy, which introduced multiple synonymous base mutations in the region that had to be patched in the RT template. The PDel/Syn strategy achieved an average of 58.1% deletion efficiency at six endogenous targets, which was higher than the PDel strategy. The strategies presented in this study contribute to achieving more accurate and flexible deletions in transgenic rice plants.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s42994-024-00153-9.</p>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Developing a CRISPR/FrCas9 system for core promoter editing in rice 开发用于水稻核心启动子编辑的 CRISPR/FrCas9 系统
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-22 DOI: 10.1007/s42994-024-00157-5
Hui Wang, Jian Ding, Jingyan Zhu, Xiaoshuang Liu, Rongfang Xu, R. Qin, Dongfang Gu, Min Li, P. Wei, Juan Li
{"title":"Developing a CRISPR/FrCas9 system for core promoter editing in rice","authors":"Hui Wang, Jian Ding, Jingyan Zhu, Xiaoshuang Liu, Rongfang Xu, R. Qin, Dongfang Gu, Min Li, P. Wei, Juan Li","doi":"10.1007/s42994-024-00157-5","DOIUrl":"https://doi.org/10.1007/s42994-024-00157-5","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140676941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural GmACO1 allelic variations confer drought tolerance and influence nodule formation in soybean 天然 GmACO1 等位基因变异赋予大豆耐旱性并影响其结核形成
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-18 DOI: 10.1007/s42994-024-00160-w
Zhifang Zhang, Junkui Ma, Xia Yang, Shan Liang, Yucheng Liu, Yaqin Yuan, Qianjin Liang, Yanting Shen, Guo-an Zhou, Min Zhang, Zhixi Tian, Shulin Liu
{"title":"Natural GmACO1 allelic variations confer drought tolerance and influence nodule formation in soybean","authors":"Zhifang Zhang, Junkui Ma, Xia Yang, Shan Liang, Yucheng Liu, Yaqin Yuan, Qianjin Liang, Yanting Shen, Guo-an Zhou, Min Zhang, Zhixi Tian, Shulin Liu","doi":"10.1007/s42994-024-00160-w","DOIUrl":"https://doi.org/10.1007/s42994-024-00160-w","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140688805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot 优化原生质体分离和转染断点：加速单子叶植物和双子叶植物中 Cas9/sgRNA 的裂解效率验证
IF 3.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-15 DOI: 10.1007/s42994-024-00139-7
Debasmita Panda, S. Karmakar, Manaswini Dash, Swagat Kumar Tripathy, Priya Das, Sagar Banerjee, Yiping Qi, Sanghamitra Samantaray, Pradipta Kumar Mohapatra, M. J. Baig, K. Molla
{"title":"Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot","authors":"Debasmita Panda, S. Karmakar, Manaswini Dash, Swagat Kumar Tripathy, Priya Das, Sagar Banerjee, Yiping Qi, Sanghamitra Samantaray, Pradipta Kumar Mohapatra, M. J. Baig, K. Molla","doi":"10.1007/s42994-024-00139-7","DOIUrl":"https://doi.org/10.1007/s42994-024-00139-7","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140702659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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