aBIOTECH最新文献

筛选
英文 中文
Early surveillance of rice bakanae disease using deep learning and hyperspectral imaging 利用深度学习和高光谱成像对水稻包虫病进行早期监测
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-21 DOI: 10.1007/s42994-024-00169-1
Sishi Chen, Xuqi Lu, Hongda Fang, Anand Babu Perumal, Ruyue Li, Lei Feng, Mengcen Wang, Yufei Liu
{"title":"Early surveillance of rice bakanae disease using deep learning and hyperspectral imaging","authors":"Sishi Chen,&nbsp;Xuqi Lu,&nbsp;Hongda Fang,&nbsp;Anand Babu Perumal,&nbsp;Ruyue Li,&nbsp;Lei Feng,&nbsp;Mengcen Wang,&nbsp;Yufei Liu","doi":"10.1007/s42994-024-00169-1","DOIUrl":"10.1007/s42994-024-00169-1","url":null,"abstract":"<div><p>Bakanae disease, caused by <i>Fusarium fujikuroi</i>, poses a significant threat to rice production and has been observed in most rice-growing regions. The disease symptoms caused by different pathogens may vary, including elongated and weak stems, slender and yellow leaves, and dwarfism, as example. Bakanae disease is likely to cause necrosis of diseased seedlings, and it may cause a large area of infection in the field through the transmission of conidia. Therefore, early disease surveillance plays a crucial role in securing rice production. Traditional monitoring methods are both time-consuming and labor-intensive and cannot be broadly applied. In this study, a combination of hyperspectral imaging technology and deep learning algorithms were used to achieve in situ detection of rice seedlings infected with bakanae disease. Phenotypic data were obtained on the 9th, 15th, and 21st day after rice infection to explore the physiological and biochemical performance, which helps to deepen the research on the disease mechanism. Hyperspectral data were obtained over these same periods of infection, and a deep learning model, named Rice Bakanae Disease-Visual Geometry Group (RBD-VGG), was established by leveraging hyperspectral imaging technology and deep learning algorithms. Based on this model, an average accuracy of 92.2% was achieved on the 21st day of infection. It also achieved an accuracy of 79.4% as early as the 9th day. Universal characteristic wavelengths were extracted to increase the feasibility of using portable spectral equipment for field surveillance. Collectively, the model offers an efficient and non-destructive surveillance methodology for monitoring bakanae disease, thereby providing an efficient avenue for disease prevention and control.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00169-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141113777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Double-barreled defense: dual ent-miltiradiene synthases in most rice cultivars 双管齐下的防御:大多数水稻栽培品种的双重ent-miltiradiene合成酶
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-20 DOI: 10.1007/s42994-024-00167-3
Yiling Feng, Tristan Weers, Reuben J. Peters
{"title":"Double-barreled defense: dual ent-miltiradiene synthases in most rice cultivars","authors":"Yiling Feng,&nbsp;Tristan Weers,&nbsp;Reuben J. Peters","doi":"10.1007/s42994-024-00167-3","DOIUrl":"10.1007/s42994-024-00167-3","url":null,"abstract":"<div><p>Rice (<i>Oryza sativa</i>) produces numerous diterpenoid phytoalexins that are important in defense against pathogens. Surprisingly, despite extensive previous investigations, a major group of such phytoalexins, the abietoryzins, were only recently reported. These aromatic abietanes are presumably derived from <i>ent</i>-miltiradiene, but such biosynthetic capacity has not yet been reported in <i>O. sativa</i>. While wild rice has been reported to contain such an enzyme, specifically <i>ent</i>-kaurene synthase-like 10 (KSL10), the only characterized ortholog from <i>O. sativa</i> (<i>OsKSL10</i>), specifically from the well-studied cultivar (cv.) Nipponbare, instead has been shown to make <i>ent</i>-sandaracopimaradiene, precursor to the oryzalexins. Notably, in many other cultivars, <i>OsKSL10</i> is accompanied by a tandem duplicate, termed here <i>OsKSL14</i>. Biochemical characterization of OsKLS14 from cv. Kitaake demonstrates that this produces the expected abietoryzin precursor <i>ent</i>-miltiradiene. Strikingly, phylogenetic analysis of <i>OsKSL10</i> across the rice pan-genome reveals that from cv. Nipponbare is an outlier, whereas the alleles from most other cultivars group with those from wild rice, suggesting that these also might produce <i>ent</i>-miltiradiene. Indeed, OsKSL10 from cv. Kitaake exhibits such activity as well, consistent with its production of abietoryzins but not oryzalexins. Similarly consistent with these results is the lack of abietoryzin production by cv. Nipponbare. Although their equivalent product outcome might suggest redundancy, <i>OsKSL10</i> and <i>OsKSL14</i> were observed to exhibit distinct expression patterns, indicating such differences may underlie retention of these duplicated genes. Regardless, the results reported here clarify abietoryzin biosynthesis and provide insight into the evolution of rice diterpenoid phytoalexins.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00167-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141118531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases C 端框移突变可产生具有发育缺陷的可行基因敲除突变体,这三种蛋白激酶至关重要
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-15 DOI: 10.1007/s42994-024-00165-5
Yun Zhang, Miao-Miao Cui, Run-Nan Ke, Yue-Dan Chen, Kabin Xie
{"title":"C-terminal frameshift mutations generate viable knockout mutants with developmental defects for three essential protein kinases","authors":"Yun Zhang,&nbsp;Miao-Miao Cui,&nbsp;Run-Nan Ke,&nbsp;Yue-Dan Chen,&nbsp;Kabin Xie","doi":"10.1007/s42994-024-00165-5","DOIUrl":"10.1007/s42994-024-00165-5","url":null,"abstract":"<div><p>Loss-of-function mutants are fundamental resources for gene function studies. However, it is difficult to generate viable and heritable knockout mutants for essential genes. Here, we show that targeted editing of the C-terminal sequence of the embryo lethal gene <i>MITOGEN-ACTIVATED PROTEIN KINASES 1</i> (<i>OsMPK1</i>) results in weak mutants. This C-terminal-edited osmpk1 mutants displayed severe developmental defects and altered disease resistance but generated tens of viable seeds that inherited the mutations. Using the same C-terminal editing approach, we also obtained viable mutants for a wall-associated protein kinase (Os07g0493200) and a leucine-rich repeat receptor-like protein kinase (Os01g0239700), while the null mutations of these genes were lethal. These data suggest that protein kinase activity could be reduced by introducing frameshift mutations adjacent to the C-terminus, which could generate valuable resources for gene function studies and tune protein kinase activity for signaling pathway engineering.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00165-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis 玉米转录因子 ZmbHLH36 的异源表达可增强拟南芥的非生物胁迫耐受性
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00159-3
Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, Huawen Zou, Zhongyi Wu, Chun Zhang
{"title":"Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis","authors":"Zhenggang Dai,&nbsp;Keyong Zhao,&nbsp;Dengyu Zheng,&nbsp;Siyu Guo,&nbsp;Huawen Zou,&nbsp;Zhongyi Wu,&nbsp;Chun Zhang","doi":"10.1007/s42994-024-00159-3","DOIUrl":"10.1007/s42994-024-00159-3","url":null,"abstract":"<div><p>Basic helix-loop-helix (bHLH) transcription factors are widely distributed in eukaryotes, and in plants, they regulate many biological processes, such as cell differentiation, development, metabolism, and stress responses. Few studies have focused on the roles of bHLH transcription factors in regulating growth, development, and stress responses in maize (<i>Zea mays</i>), even though such information would greatly benefit maize breeding programs. In this study, we cloned the maize transcription factor gene <i>ZmbHLH36</i> (Gene ID: 100193615, GRMZM2G008691). ZmbHLH36 possesses conserved domains characteristic of the bHLH family. RT-qPCR analysis revealed that <i>ZmbHLH36</i> was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress conditions. Transgenic <i>Arabidopsis</i> (<i>Arabidopsis thaliana</i>) plants heterologously expressing <i>ZmbHLH36</i> had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L<sup>−1</sup> NaCl treatment as well as 0.2 mol L<sup>−1</sup> mannitol treatment. Phenotypic analysis of soil-grown plants under stress showed that transgenic <i>Arabidopsis</i> plants harboring <i>ZmbHLH36</i> exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic plants. Malondialdehyde contents were lower and peroxidase activity was higher in <i>ZmbHLH36</i>-expressing <i>Arabidopsis</i> plants than in the corresponding non-transgenic plants. ZmbHLH36 localized to the nucleus when expressed in maize protoplasts. This study provides a systematic analysis of the effects of ZmbHLH36 on root growth, development, and stress responses in transgenic <i>Arabidopsis</i>, laying a foundation for further analysis of its roles and molecular mechanisms in maize.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00159-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140982769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Strategies for adaptation to high light in plants 植物适应强光的策略
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-13 DOI: 10.1007/s42994-024-00164-6
Man Zhang, Yu Ming, Hong-Bin Wang, Hong-Lei Jin
{"title":"Strategies for adaptation to high light in plants","authors":"Man Zhang,&nbsp;Yu Ming,&nbsp;Hong-Bin Wang,&nbsp;Hong-Lei Jin","doi":"10.1007/s42994-024-00164-6","DOIUrl":"10.1007/s42994-024-00164-6","url":null,"abstract":"<div><p>Plants absorb light energy for photosynthesis via photosystem complexes in their chloroplasts. However, excess light can damage the photosystems and decrease photosynthetic output, thereby inhibiting plant growth and development. Plants have developed a series of light acclimation strategies that allow them to withstand high light. In the first line of defense against excess light, leaves and chloroplasts move away from the light and the plant accumulates compounds that filter and reflect the light. In the second line of defense, known as photoprotection, plants dissipate excess light energy through non-photochemical quenching, cyclic electron transport, photorespiration, and scavenging of excess reactive oxygen species. In the third line of defense, which occurs after photodamage, plants initiate a cycle of photosystem (mainly photosystem II) repair. In addition to being the site of photosynthesis, chloroplasts sense stress, especially light stress, and transduce the stress signal to the nucleus, where it modulates the expression of genes involved in the stress response. In this review, we discuss current progress in our understanding of the strategies and mechanisms employed by plants to withstand high light at the whole-plant, cellular, physiological, and molecular levels across the three lines of defense.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00164-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140984916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploiting viral vectors to deliver genome editing reagents in plants 利用病毒载体为植物提供基因组编辑试剂
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-08 DOI: 10.1007/s42994-024-00147-7
Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong, Jianmin Wan
{"title":"Exploiting viral vectors to deliver genome editing reagents in plants","authors":"Yilin Shen,&nbsp;Tao Ye,&nbsp;Zihan Li,&nbsp;Torotwa Herman Kimutai,&nbsp;Hao Song,&nbsp;Xiaoou Dong,&nbsp;Jianmin Wan","doi":"10.1007/s42994-024-00147-7","DOIUrl":"10.1007/s42994-024-00147-7","url":null,"abstract":"<div><p>Genome editing holds great promise for the molecular breeding of plants, yet its application is hindered by the shortage of simple and effective means of delivering genome editing reagents into plants. Conventional plant transformation-based methods for delivery of genome editing reagents into plants often involve prolonged tissue culture, a labor-intensive and technically challenging process for many elite crop cultivars. In this review, we describe various virus-based methods that have been employed to deliver genome editing reagents, including components of the CRISPR/Cas machinery and donor DNA for precision editing in plants. We update the progress in these methods with recent successful examples of genome editing achieved through virus-based delivery in different plant species, highlight the advantages and limitations of these delivery approaches, and discuss the remaining challenges.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00147-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141001110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Decoding the microbiome for sustainable agriculture 解码微生物组,促进可持续农业
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-07 DOI: 10.1007/s42994-024-00162-8
Kai Sun, Wei Zhang, Xiaolin Wang, Chuan-Chao Dai
{"title":"Decoding the microbiome for sustainable agriculture","authors":"Kai Sun,&nbsp;Wei Zhang,&nbsp;Xiaolin Wang,&nbsp;Chuan-Chao Dai","doi":"10.1007/s42994-024-00162-8","DOIUrl":"10.1007/s42994-024-00162-8","url":null,"abstract":"<div><p>Root-associated microbiota profoundly affect crop health and productivity. Plants can selectively recruit beneficial microbes from the soil and actively balance microbe-triggered plant-growth promotion and stress tolerance enhancement. The cost associated with this is the root-mediated support of a certain number of specific microbes under nutrient limitation. Thus, it is important to consider the dynamic changes in microbial quantity when it comes to nutrient condition-induced root microbiome reassembly. Quantitative microbiome profiling (QMP) has recently emerged as a means to estimate the specific microbial load variation of a root microbiome (instead of the traditional approach quantifying relative microbial abundances) and data from the QMP approach can be more closely correlated with plant development and/or function. However, due to a lack of detailed-QMP data, how soil nutrient conditions affect quantitative changes in microbial assembly of the root-associated microbiome remains poorly understood. A recent study quantified the dynamics of the soybean root microbiome, under unbalanced fertilization, using QMP and provided data on the use of specific synthetic communities (SynComs) for sustaining crop productivity. In this editorial, we explore potential opportunities for utilizing QMP to decode the microbiome for sustainable agriculture.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Publisher Correction: Removal of the C4-domain preserves the drought tolerance enhanced by CsMYB4a and eliminates the negative impact of this transcription factor on plant growth 错误:出版者更正:移除 C4 域可保留 CsMYB4a 增强的耐旱性,并消除该转录因子对植物生长的负面影响。
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-05-06 DOI: 10.1007/s42994-024-00163-7
Mingzhuo Li, Guoliang Ma, Xiu Li, Lili Guo, Yanzhi Li, Yajun Liu, Wenzhao Wang, Xiaolan Jiang, De-Yu Xie, Liping Gao, Tao Xia
{"title":"Publisher Correction: Removal of the C4-domain preserves the drought tolerance enhanced by CsMYB4a and eliminates the negative impact of this transcription factor on plant growth","authors":"Mingzhuo Li,&nbsp;Guoliang Ma,&nbsp;Xiu Li,&nbsp;Lili Guo,&nbsp;Yanzhi Li,&nbsp;Yajun Liu,&nbsp;Wenzhao Wang,&nbsp;Xiaolan Jiang,&nbsp;De-Yu Xie,&nbsp;Liping Gao,&nbsp;Tao Xia","doi":"10.1007/s42994-024-00163-7","DOIUrl":"10.1007/s42994-024-00163-7","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Current overview on the genetic basis of key genes involved in soybean domestication 更正:大豆驯化关键基因的遗传基础概述。
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-30 DOI: 10.1007/s42994-024-00161-9
Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu
{"title":"Correction: Current overview on the genetic basis of key genes involved in soybean domestication","authors":"Sijia Lu,&nbsp;Chao Fang,&nbsp;Jun Abe,&nbsp;Fanjiang Kong,&nbsp;Baohui Liu","doi":"10.1007/s42994-024-00161-9","DOIUrl":"10.1007/s42994-024-00161-9","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficient and precise genomic deletion in rice using enhanced prime editing 利用增强的质粒编辑技术高效、精确地删除水稻基因组。
IF 4.6 4区 农林科学
aBIOTECH Pub Date : 2024-04-29 DOI: 10.1007/s42994-024-00153-9
Mengyuan Liu, Xiang Zhang, Wen Xu, Guiting Kang, Ya Liu, Xinxiang Liu, Wen Ren, Jiuran Zhao, Jinxiao Yang
{"title":"Efficient and precise genomic deletion in rice using enhanced prime editing","authors":"Mengyuan Liu,&nbsp;Xiang Zhang,&nbsp;Wen Xu,&nbsp;Guiting Kang,&nbsp;Ya Liu,&nbsp;Xinxiang Liu,&nbsp;Wen Ren,&nbsp;Jiuran Zhao,&nbsp;Jinxiao Yang","doi":"10.1007/s42994-024-00153-9","DOIUrl":"10.1007/s42994-024-00153-9","url":null,"abstract":"<div><p>Efficient and precise genomic deletion shows promise for investigating the function of proteins in plant research and enhancing agricultural traits. In this study, we tested the PRIME-Del (PDel) strategy using a pair of prime editing guide RNAs (pegRNAs) that targeted opposite DNA strands and achieved an average deletion efficiency of 55.8% for 60 bp fragment deletions at six endogenous targets. Moreover, as high as 84.2% precise deletion efficiency was obtained for a 2000 bp deletion at the <i>OsGS1</i> site in transgenic rice plants. To add the bases that were unintentionally deleted between the two nicking sequences, we used the PDel/Syn strategy, which introduced multiple synonymous base mutations in the region that had to be patched in the RT template. The PDel/Syn strategy achieved an average of 58.1% deletion efficiency at six endogenous targets, which was higher than the PDel strategy. The strategies presented in this study contribute to achieving more accurate and flexible deletions in transgenic rice plants.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信