aBIOTECH最新文献_第2页

Motif-Hi-C: A motif-based framework for rapid quality control of Hi-C data. Motif-Hi-C：基于motif的Hi-C数据快速质量控制框架。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-03-18 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100040

Dashuai Kong, Yuhui Wang, Yueting Tang, Yunhan Tian, Shulin Yang, Yanfang Wang, Yubo Zhang, Siyuan Kong

{"title":"Motif-Hi-C: A motif-based framework for rapid quality control of Hi-C data.","authors":"Dashuai Kong, Yuhui Wang, Yueting Tang, Yunhan Tian, Shulin Yang, Yanfang Wang, Yubo Zhang, Siyuan Kong","doi":"10.1016/j.abiote.2026.100040","DOIUrl":"10.1016/j.abiote.2026.100040","url":null,"abstract":"Hi-C technology has become indispensable for studying three-dimensional (3D) genome organization; however, the substantial variability in existing genome analysis tools poses challenges for efficient, accurate data processing. Here, we present Motif-Hi-C, an innovative computational framework that leverages the intrinsic sequence signatures of Hi-C chimeric reads to enable rapid, reliable quality control. Through systematic benchmarking of four mainstream tools (HiC-Pro, HiCUP, Juicer, and HiCExplorer) across diverse Hi-C datasets, we identified critical trade-offs between processing speed and analytical precision. Motif-Hi-C addresses these limitations by implementing a motif-aware classification system that achieves faster processing with more valid interacting rmdup/total read pairs. Key innovations include (1) automated detection of ligation-motif signatures in raw sequencing data, (2) category-specific parallel processing pipelines, and (3) dynamic quality thresholds adapted to different experimental protocols. Validation across multiple cell types and restriction enzymes demonstrated robust performance, with particular advantages for large-scale consortium datasets. This framework establishes a new paradigm for efficient quality assessment in 3D genomics studies, seamlessly integrating with downstream chromatin interaction analyses.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100040"},"PeriodicalIF":8.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13133929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperspectral phenotyping reveals the genetic basis of grain quality in rice. 高光谱表型揭示了水稻籽粒品质的遗传基础。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-03-18 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100039

Weijuan Hu, Xiaoqian Chen, Jianping Yu, Jie Deng, Xiao Ye, Changquan Zhang, Shuoxun Wang, Wenzhen Song, Yafeng Ye, Xiuhua Gao, Wanneng Yang, Qiang Liu, Xiangdong Fu, Kun Wu

{"title":"Hyperspectral phenotyping reveals the genetic basis of grain quality in rice.","authors":"Weijuan Hu, Xiaoqian Chen, Jianping Yu, Jie Deng, Xiao Ye, Changquan Zhang, Shuoxun Wang, Wenzhen Song, Yafeng Ye, Xiuhua Gao, Wanneng Yang, Qiang Liu, Xiangdong Fu, Kun Wu","doi":"10.1016/j.abiote.2026.100039","DOIUrl":"10.1016/j.abiote.2026.100039","url":null,"abstract":"Rice (Oryza sativa) grain quality is an important breeding target, yet its genetic basis remains incompletely understood. In this study, we integrated hyperspectral phenotyping with genome-wide association study (GWAS) to investigate apparent amylose content (AAC) and protein content (PC) in 241 modern rice varieties. Using a visible-shortwave infrared hyperspectral system combined with optimized preprocessing and machine-learning pipelines, we achieved accurate predictions for AAC (R 2 = 0.97) and PC (R 2 = 0.92). Hyperspectral-based GWAS identified both known loci and previously unreported genetic associations. For AAC, qAAC (780.791nm) -1-3 was mapped to the Green Revolution gene SD1, showing that the sd1 allele increases AAC while conferring high yields. For PC, we identified qPC (1998.98nm) -5-1 and confirmed GW5 as the causal gene, linking the high-yielding gw5 allele with high grain PC. Hyperspectral features outperformed traditional measurements, enhancing the detection of genetic signals. This study provides an efficient strategy for elucidating the genomic architecture of complex grain-quality traits.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100039"},"PeriodicalIF":8.5,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13087691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The oxidation of ABI4 by RBOHD-derived reactive oxygen species integrates redox signaling into abscisic-acid and drought-stress responses. rbohd衍生的活性氧对ABI4的氧化将氧化还原信号整合到脱落酸和干旱胁迫响应中。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-03-06 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100037

Zhenglin Ge, Tiantian Wu, Zhengyao Lin, Sisong Lai, Guojing Chen, Lihui Xiao, Jing Zhang, Kailu Zhang, Heng Zhou, Yanjie Xie

{"title":"The oxidation of ABI4 by RBOHD-derived reactive oxygen species integrates redox signaling into abscisic-acid and drought-stress responses.","authors":"Zhenglin Ge, Tiantian Wu, Zhengyao Lin, Sisong Lai, Guojing Chen, Lihui Xiao, Jing Zhang, Kailu Zhang, Heng Zhou, Yanjie Xie","doi":"10.1016/j.abiote.2026.100037","DOIUrl":"10.1016/j.abiote.2026.100037","url":null,"abstract":"The phytohormone abscisic acid (ABA) induces stomatal closure and facilitates plant adaptation to drought stress. Reactive oxygen species (ROS) produced by the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN D (RBOHD) are essential components of ABA signal transduction. However, the molecular mechanisms by which ABA influences ROS production, and how ROS signaling is integrated into the ABA signaling network, are poorly understood. Here, we report that the transcription factor ABSCISIC ACID INSENSITIVE 4 (ABI4) acts upstream of RBOHD to regulate ABA-induced ROS production and stomatal closure in Arabidopsis (Arabidopsis thaliana). We determined that ABA-induced RBOHD expression and ROS production were significantly attenuated in the abi4 mutant. In the wild type, an ABA-triggered ROS burst promoted the sulfenylation of ABI4 at Cys250, which enhanced its DNA binding and transactivation activity toward RBOHD, thereby regulating ABA signaling. The sulfenylation-dependent regulation of ABI4 was required for ABA-induced stomatal closure and greatly contributed to plant drought tolerance. These findings uncover a redox-dependent feedback loop in which ABI4 oxidation fine-tunes ABA responses and drought tolerance by dynamically regulating RBOHD-mediated ROS production. This post-translational regulatory mechanism for ABA signaling establishes a molecular framework explaining the crosstalk between ABA and ROS in the regulation of stomatal closure and drought resilience.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100037"},"PeriodicalIF":8.5,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13019803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-enzymatic acetylation of lysine residues affects the stability of Rubisco activase. 赖氨酸残基的非酶乙酰化影响Rubisco激活酶的稳定性。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-02-24 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100036

Lili Yang, Yanjun Li, Hui Hong, Chunmei Luo, Xiang Gao, Zhan Shu, Hua-Ling Mi, Gen-Yun Chen, Mingnan Qu

{"title":"Non-enzymatic acetylation of lysine residues affects the stability of Rubisco activase.","authors":"Lili Yang, Yanjun Li, Hui Hong, Chunmei Luo, Xiang Gao, Zhan Shu, Hua-Ling Mi, Gen-Yun Chen, Mingnan Qu","doi":"10.1016/j.abiote.2026.100036","DOIUrl":"10.1016/j.abiote.2026.100036","url":null,"abstract":"Post-translational modifications of non-histone proteins are critical for plant adaptation to fluctuating environmental conditions. Rubisco activase (RCA), a chloroplastic ATPase, plays a crucial role in CO2 fixation by activating ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In this study, we determined that RCA isolated from tobacco (Nicotiana tabacum) leaves is acetylated at multiple lysine residues, with K126 and K164 being key acetylation sites. The acetylation level of RCA varies under different light regimes (day vs. night) and heat-stress conditions. RCA undergoes non-enzymatic acetylation in vitro through direct interactions with various metabolites, particularly acetyl-CoA (Ac-CoA). Using deuterium-labeled Ac-CoA, we confirmed that lysine residues K126 and K164 are acetylated; this acetylation event leads to a significant reduction in the ATPase activity of RCA, with only a minor impact on Rubisco activation. Additionally, higher acetylation levels of RCA accelerate its degradation in chloroplasts, a process independent of ubiquitination. Our findings uncover a previously unknown function of protein acetylation in regulating RCA stability, which is essential for modulating carbon assimilation efficiency in plants under varying energy conditions.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100036"},"PeriodicalIF":8.5,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12990336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering PRRs: A new path to durable resistance. 工程PRRs：持久抵抗的新途径。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-02-12 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100028

Dongjiao Wang, Zhankun Li, Wentao Yang, Youxiong Que, Ming Chang

引用次数: 0

Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design. 通过蛋白质生成模型和能量优化设计构建转座酶ISDra2 TnpB的高效突变体。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-02-06 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100027

Feifei Guan, Yekun Ding, Guoshun Xu, Lingxi Fan, Xiaofeng Gu, Yuan Wang, Huiying Luo, Bin Yao, Jian Tian

{"title":"Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design.","authors":"Feifei Guan, Yekun Ding, Guoshun Xu, Lingxi Fan, Xiaofeng Gu, Yuan Wang, Huiying Luo, Bin Yao, Jian Tian","doi":"10.1016/j.abiote.2026.100027","DOIUrl":"10.1016/j.abiote.2026.100027","url":null,"abstract":"Transposon-associated TnpB is a compact and versatile gene editor that holds significant promise in the life sciences. However, current engineering strategies for TnpB are limited, resulting in low cleavage efficiency in mammalian cells that restricts its broader use. In this study, we developed a method termed TnpB-GMRE, which integrates a generative protein model for TnpB with a virtual screening pipeline based on the minimum recovery rate and energy minimization. From 100,000 generated sequences, we selected the top five candidates for experimental assessment. Without the use of enrichment strategies such as flow cytometry or antibiotic selection, three of the five mutants displayed higher editing activity across four target sites. Among them, the TnpB-TD mutant achieved the highest editing activity (17.7%), representing a 50% increase compared to the original ISDra2 TnpB from Deinococcus radiodurans. In addition, the TnpB-TD mutant exhibited greater diversity in editing profiles and superior efficiency in deleting long fragments (>10 bp) relative to the intact ISDra2 TnpB. Molecular dynamics simulations revealed that compared to the wild type, the TnpB-TD mutant adopted a greater number of low-energy conformational states and displayed an increased positive charge on its surface, suggestive of a stabilized structure that may underlie its enhanced editing performance. This strategy provides a framework for optimizing compact nucleases and broadens the available toolkit for gene-editing applications.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100027"},"PeriodicalIF":8.5,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the efficiency of nuclease-based prime editing in rice with the Tf1 reverse transcriptase. 利用Tf1逆转录酶提高水稻核酸酶引物编辑效率。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-02-05 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100026

Guigen Ma, Hao Xu, Sujie Zhang, Xueqi Li, Jiaqiang Liu, Jiayue Xie, Fang Yan, Huanbin Zhou

{"title":"Enhancing the efficiency of nuclease-based prime editing in rice with the Tf1 reverse transcriptase.","authors":"Guigen Ma, Hao Xu, Sujie Zhang, Xueqi Li, Jiaqiang Liu, Jiayue Xie, Fang Yan, Huanbin Zhou","doi":"10.1016/j.abiote.2026.100026","DOIUrl":"10.1016/j.abiote.2026.100026","url":null,"abstract":"The development of efficient and precise genome-editing tools is crucial for advancing functional genomics and improving crops. Our previously established nuclease-mediated prime editing (NM-PE) system, which combines the SpCas9 nuclease with prime editing based on microhomology-mediated end joining, enables the seamless insertion of small DNA fragments into plant genomes to add tags to genes of interest. However, the efficiency of 3 × FLAG sequence insertion via NM-PE requires further improvement. Here, we report a significant optimization of this system by replacing the M-MLV reverse transcriptase (RT) with evolved variants of the retrotransposon RT Tf1 derived from the mammalian PE6 system. Through codon optimization, we generated the evoTf1M4 variant, which substantially enhanced the efficiency of NM-PE. The optimized construct rPE20aV3 achieved up to 18.75% precise insertion of a 66-bp 3 × FLAG sequence at endogenous loci, representing a three-fold improvement over the original NM-PE system. Our results demonstrate that Tf1-aided optimization of NM-PE serves as an efficient platform for seamless insertion of a 3 × FLAG sequence in rice, offering broad potential for advanced genome engineering in plants.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100026"},"PeriodicalIF":8.5,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pathogen-induced translational shift enhances plant disease resistance without obvious fitness costs. 病原菌诱导的转译转移增强了植物的抗病性，但没有明显的适应性代价。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-02-03 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100025

Yuzhen Mei, Xiaofei Cheng, Yaqin Wang, Fangfang Li, Xueping Zhou

{"title":"A pathogen-induced translational shift enhances plant disease resistance without obvious fitness costs.","authors":"Yuzhen Mei, Xiaofei Cheng, Yaqin Wang, Fangfang Li, Xueping Zhou","doi":"10.1016/j.abiote.2026.100025","DOIUrl":"10.1016/j.abiote.2026.100025","url":null,"abstract":"Exploring immune mechanisms in plants opens new avenues for engineering disease resistance in crops. Approaches such as the ectopic expression of resistance genes are frequently associated with fitness costs, rendering the resulting germplasm less desirable for agricultural applications. Inducible production of resistance factors is one potential workaround for this problem. In this study, we determined that the 5' untranslated region (5' UTR) of Arabidopsis (Arabidopsis thaliana) PI4KIII β1 undergoes a translational shift that alters its translation initiation site upon the activation of immunity. Based on this discovery, we developed a PI4KIII β1 5' UTR cassette to specifically regulate the expression of the hypersensitive induced reaction gene HIR1, which encodes a cell death-inducing protein. Under normal conditions, ribosomes initiated translation from the first start codon in the PI4KIII β1 5' UTR cassette; however, upon pathogen challenge, the ribosomes bypassed this site and instead initiated translation from a downstream start codon, enabling HIR1 expression. Transgenic Arabidopsis plants harboring the PI4KIII β1 5' UTR cassette driving HIR1 exhibited enhanced disease resistance without detectable changes in key agricultural traits. Therefore, precisely regulating translation initiation through leaky ribosome scanning represents a promising strategy for developing pathogen-resistant crops without obvious fitness costs.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100025"},"PeriodicalIF":8.5,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visual marker-assisted gene editing of MLO confers powdery mildew resistance in Nicotiana alata. MLO的视觉标记辅助基因编辑赋予烟叶白粉病抗性。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-01-18 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100024

Hua-Yin Liu, Xin-Yao Huang, Jin-Wang, Yan-Qun Zhang, Jing Li, Ling-Wang Kong, Shi-Ping Zhou, Qiu-Fen Hu, Wei-Guang Wang

{"title":"Visual marker-assisted gene editing of MLO confers powdery mildew resistance in Nicotiana alata.","authors":"Hua-Yin Liu, Xin-Yao Huang, Jin-Wang, Yan-Qun Zhang, Jing Li, Ling-Wang Kong, Shi-Ping Zhou, Qiu-Fen Hu, Wei-Guang Wang","doi":"10.1016/j.abiote.2026.100024","DOIUrl":"10.1016/j.abiote.2026.100024","url":null,"abstract":"Nicotiana alata is a high-value floricultural crop, but its widely cultivated commercial cultivar 'Jasmine' is highly susceptible to powdery mildew under low-light indoor conditions. This susceptibility results in leaf mold, yellowing, and wilting, along with a sharp decline in ornamental value. To address this issue and reduce fungicide reliance, we developed the first gene-editing system for N. alata featuring antibiotic marker-free visual identification. This system employs dual-visual markers for rapid positive screening using anthocyanin accumulation to indicate successful transformation and albino phenotypes resulting from editing of NalaPDS to signal successful gene editing. To enhance the horticultural traits of N. alata, we knocked out NalaMLO, resulting in T1 and T2 plants with significantly greater resistance to powdery mildew. Headspace solid-phase microextraction-gas chromatography-mass spectrometry identified 179 floral volatiles, with key odorants such as linalool, phenylethyl alcohol, and d-limonene remaining unaffected in the edited lines. Transcriptomics, metabolomics, and proteomics analyses revealed that the resistance phenotype may be mediated by the coordination of multiple pathways, including the MAPK signaling pathway, starch and sucrose metabolism, fructose and mannose metabolism, and phenylpropanoid biosynthesis. This approach effectively reduces disease-induced losses in the N. alata cultivar Jasmine and will accelerate the deployment of disease-resistance traits in ornamental Solanaceae crops.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100024"},"PeriodicalIF":8.5,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A class II 5-enolpyruvylshikimate-3-phosphate synthase from Pseudomonas P818 confers robust glyphosate tolerance in transgenic plants. 来自P818假单胞菌的II类5-烯醇丙酮酰莽草酸-3-磷酸合成酶赋予转基因植物强大的草甘膦耐受性。

IF 8.5 4区农林科学

aBIOTECH Pub Date : 2026-01-17 eCollection Date: 2026-06-01 DOI: 10.1016/j.abiote.2026.100023

Yuhao Su, Jie Cao, Yan Liu, Xiaodong Xie, Tianyong Zhao, Gaoyi Cao, Yunjun Liu

{"title":"A class II 5-enolpyruvylshikimate-3-phosphate synthase from Pseudomonas P818 confers robust glyphosate tolerance in transgenic plants.","authors":"Yuhao Su, Jie Cao, Yan Liu, Xiaodong Xie, Tianyong Zhao, Gaoyi Cao, Yunjun Liu","doi":"10.1016/j.abiote.2026.100023","DOIUrl":"10.1016/j.abiote.2026.100023","url":null,"abstract":"Glyphosate is a widely used herbicide that targets 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), blocking the shikimate pathway and leading to plant death. The discovery of novel EPSPS genes is key to engineering glyphosate tolerance in crops. In this study, we isolated the glyphosate-tolerant bacterial strain Pseudomonas P818 from glyphosate-contaminated soil and cloned its class II EPSPS gene (818-EPSPS). Sequence and phylogenetic analyses revealed typical motifs of class II EPSPS. Kinetic characterization revealed a high Ki/Km ratio (10.4), indicating that 818-EPSPS has low affinity for glyphosate (high Ki value) while retaining high catalytic efficiency (low Km value). The heterologous expression of 818-EPSPS restored growth in E. coli strain ER2799 under glyphosate stress, confirming its functional resistance. Transgenic Arabidopsis thaliana and tobacco (Nicotiana tabacum) plants heterologously expressing 818-EPSPS exhibited strong tolerance to glyphosate, maintaining growth at concentrations that were lethal to the wild-type controls. We introduced codon-optimized 818-EPSPS into maize (Zea mays), generating stable transgenic plants. Transgenic maize line EP03, carrying a single-copy insertion, showed robust tolerance to up to four times the recommended dosage of glyphosate in field trials. Genomic sequencing revealed that the T-DNA in EP03 was inserted into chromosome 5 without disrupting any host genes. Our findings establish 818-EPSPS as a promising candidate for engineering glyphosate-tolerant crops and provide a new genetic resource for maize improvement and biosafety applications.","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"7 2","pages":"100023"},"PeriodicalIF":8.5,"publicationDate":"2026-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147624750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0