aBIOTECH最新文献_第10页

Transcriptome-wide N6-methyladenosine (m6A) methylation in soybean under Meloidogyne incognita infection 隐性根结线虫感染下大豆全转录组N6-甲基腺苷（m6A）甲基化

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-08-18 DOI: 10.1007/s42994-022-00077-2

Xue Han, Qianqian Shi, Ziyi He, Wenwen Song, Qingshan Chen, Zhaoming Qi

{"title":"Transcriptome-wide N6-methyladenosine (m6A) methylation in soybean under Meloidogyne incognita infection","authors":"Xue Han, Qianqian Shi, Ziyi He, Wenwen Song, Qingshan Chen, Zhaoming Qi","doi":"10.1007/s42994-022-00077-2","DOIUrl":"10.1007/s42994-022-00077-2","url":null,"abstract":"<div>N6-methyladenosine (m6A) is a reversible epigenetic modification of mRNA and other RNAs that plays a significant role in regulating gene expression and biological processes. However, m6A abundance, dynamics, and transcriptional regulatory mechanisms remain unexplored in the context of soybean resistance to Meloidogyne incognita. In this study, we performed a comparative analysis of transcriptome-wide m6A and metabolome profiles of soybean root tissues with and without M. incognita infection. Global m6A hypermethylation was widely induced in response to M. incognita infection and was enriched around the 3′ end of coding sequences and in 3′ UTR regions. There were 2069 significantly modified m6A sites, 594 differentially expressed genes, and 103 differentially accumulated metabolites between infected and uninfected roots, including coumestrol, psoralidin, and 2-hydroxyethylphosphonate. Among 101 m6A-modified DEGs, 34 genes were hypomethylated and upregulated, and 39 genes were hypermethylated and downregulated, indicating a highly negative correlation between m6A methylation and gene transcript abundance. A number of these m6A-modified DEGs, including WRKY70, ERF60, POD47 and LRR receptor-like serine/threonine-protein kinases, were involved in plant defense responses. Our study provides new insights into the critical role of m6A modification in early soybean responses to M. incognita.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 3","pages":"197 - 211"},"PeriodicalIF":3.6,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00077-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50491781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Current overview on the genetic basis of key genes involved in soybean domestication 大豆驯化关键基因的遗传基础研究进展

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-07-02 DOI: 10.1007/s42994-022-00074-5

Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu

{"title":"Current overview on the genetic basis of key genes involved in soybean domestication","authors":"Sijia Lu, Chao Fang, Jun Abe, Fanjiang Kong, Baohui Liu","doi":"10.1007/s42994-022-00074-5","DOIUrl":"10.1007/s42994-022-00074-5","url":null,"abstract":"<div>Modern crops were created through the domestication and genetic introgression of wild relatives and adaptive differentiation in new environments. Identifying the domestication-related genes and unveiling their molecular diversity provide clues for understanding how the domesticated variants were selected by ancient people, elucidating how and where these crops were domesticated. Molecular genetics and genomics have explored some domestication-related genes in soybean (Glycine max). Here, we summarize recent studies about the quantitative trait locus (QTL) and genes involved in the domestication traits, introduce the functions of these genes, clarify which alleles of domesticated genes were selected during domestication. A deeper understanding of soybean domestication could help to break the bottleneck of modern breeding by highlighting unused genetic diversity not selected in the original domestication process, as well as highlighting promising new avenues for the identification and research of important agronomic traits among different crop species.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 2","pages":"126 - 139"},"PeriodicalIF":3.6,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590488/pdf/42994_2022_Article_74.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9549837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives CRISPR/Cas技术在芸苔属作物中的应用现状与展望

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-07-02 DOI: 10.1007/s42994-022-00076-3

Jun Li, Xiaoxiao Yu, Chao Zhang, Na Li, Jianjun Zhao

{"title":"The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives","authors":"Jun Li, Xiaoxiao Yu, Chao Zhang, Na Li, Jianjun Zhao","doi":"10.1007/s42994-022-00076-3","DOIUrl":"10.1007/s42994-022-00076-3","url":null,"abstract":"<div>Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of Brassica crops.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 2","pages":"146 - 161"},"PeriodicalIF":3.6,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00076-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50440230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat CRISPR/Cas9基因编辑对水稻三个多胺摄取转运蛋白基因的破坏赋予其对除草剂百草枯的耐受性

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-06-25 DOI: 10.1007/s42994-022-00075-4

Yu-Shu Lyu, Li-Miao Cao, Wen-Qian Huang, Jian-Xiang Liu, Hai-Ping Lu

引用次数: 10

Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development 用于研究大豆生长发育和共生根瘤发育的两个组成型启动子RPS28和EIF1的特性

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-06-13 DOI: 10.1007/s42994-022-00073-6

Shengcai Chen, Yaqi Peng, Qi Lv, Jing Liu, Zhihua Wu, Haijiao Wang, Xuelu Wang

{"title":"Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development","authors":"Shengcai Chen, Yaqi Peng, Qi Lv, Jing Liu, Zhihua Wu, Haijiao Wang, Xuelu Wang","doi":"10.1007/s42994-022-00073-6","DOIUrl":"10.1007/s42994-022-00073-6","url":null,"abstract":"<div>Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits. Although several such promoters have been reported in soybean (Glycine max), few of them function at multiple growth and development stages and during nodule development. Here, we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28 (RPS28) and EUKARYOTIC TRANSLATION INITIATION FACTOR 1 (EIF1) are ideal for high expression of transgene. Through bioinformatic analysis, we determined that RPS28 and EIF1 were highly expressed during soybean growth and development, nodule development, and various biotic and abiotic stresses. Fusion of both RPS28 and EIF1 promoters, with or without their first intron, with the reporter gene β-GLUCURONIDASE (uidA) in transgenic soybean, resulted in high GUS activity in seedlings, seeds, and nodules. Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression, comparable to the soybean Ubiquitin (GmUbi) promoter. RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana. Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean, as well as in a wide variety of other plant species.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 2","pages":"99 - 109"},"PeriodicalIF":3.6,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50478062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the gene pool for soybean improvement with its wild relatives 扩大大豆及其野生亲缘改良的基因库

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-05-20 DOI: 10.1007/s42994-022-00072-7

Yongbin Zhuang, Xiaoming Li, Junmei Hu, Ran Xu, Dajian Zhang

{"title":"Expanding the gene pool for soybean improvement with its wild relatives","authors":"Yongbin Zhuang, Xiaoming Li, Junmei Hu, Ran Xu, Dajian Zhang","doi":"10.1007/s42994-022-00072-7","DOIUrl":"10.1007/s42994-022-00072-7","url":null,"abstract":"<div>Genetic diversity is a cornerstone of crop improvement, However, cultivated soybean (Glycine max) has undergone several genetic bottlenecks, including domestication in China, the introduction of landraces to other areas of the world and, latterly, selective breeding, leading to low genetic diversity the poses a major obstacle to soybean improvement. By contrast, there remains a relatively high level of genetic diversity in soybean’s wild relatives, especially the perennial soybeans (Glycine subgenus Glycine), which could serve as potential gene pools for improving soybean cultivars. Wild soybeans are phylogenetically diversified and adapted to various habitats, harboring resistance to various biotic and abiotic stresses. Advances in genome and transcriptome sequencing enable alleles associated with desirable traits that were lost during domestication of soybean to be discovered in wild soybean. The collection and conservation of soybean wild relatives and the dissection of their genomic features will accelerate soybean breeding and facilitate sustainable agriculture and food production.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 2","pages":"115 - 125"},"PeriodicalIF":3.6,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00072-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9088387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Epigenetic regulation of thermomorphogenesis in Arabidopsis thaliana 拟南芥热形态发生的表观遗传学调控

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-03-14 DOI: 10.1007/s42994-022-00070-9

Yifeng Hou, Yan Yan, Xiaofeng Cao

引用次数: 3

Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9 CRISPR/Cas9对大豆优良品种破荚不利等位基因的消除

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-03-07 DOI: 10.1007/s42994-022-00071-8

Zhihui Zhang, Jie Wang, Huaqin Kuang, Zhihong Hou, Pingping Gong, Mengyan Bai, Shaodong Zhou, Xiaolei Yao, Shikui Song, Long Yan, Yuefeng Guan

{"title":"Elimination of an unfavorable allele conferring pod shattering in an elite soybean cultivar by CRISPR/Cas9","authors":"Zhihui Zhang, Jie Wang, Huaqin Kuang, Zhihong Hou, Pingping Gong, Mengyan Bai, Shaodong Zhou, Xiaolei Yao, Shikui Song, Long Yan, Yuefeng Guan","doi":"10.1007/s42994-022-00071-8","DOIUrl":"10.1007/s42994-022-00071-8","url":null,"abstract":"<div>Pod shattering can lead to devastating yield loss of soybean and has been a negatively selected trait in soybean domestication and breeding. Nevertheless, a significant portion of soybean cultivars are still pod shattering-susceptible, limiting their regional and climatic adaptabilities. Here we performed genetic diagnosis on the shattering-susceptible trait of a national registered cultivar, Huachun6 (HC6), and found that HC6 carries the susceptible genotype of a candidate Pod dehiscence 1 (PDH1) gene, which exists in a significant portion of soybean cultivars. We next performed genome editing on PDH1 gene by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9). In T2 progenies, several transgene-free lines with pdh1 mutations were characterized without affecting major agronomic traits. The pdh1 mutation significantly improved the pod shattering resistance which is associated with aberrant lignin distribution in inner sclerenchyma. Our work demonstrated that precision breeding by genome editing on PDH1 holds great potential for precisely improving pod shattering resistance and adaptability of soybean cultivars.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"3 2","pages":"110 - 114"},"PeriodicalIF":3.6,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-022-00071-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40653714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Temperature-mediated regulation of flowering time in Arabidopsis thaliana 温度介导的拟南芥开花时间调控

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-03-03 DOI: 10.1007/s42994-022-00069-2

C. Maddie Brightbill, Sibum Sung

引用次数: 3

Post-translational modification: a strategic response to high temperature in plants 转化后修饰：植物对高温的战略性反应

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2022-02-15 DOI: 10.1007/s42994-021-00067-w

Danlu Han, Zhibo Yu, Jianbin Lai, Chengwei Yang

引用次数: 8