aBIOTECH最新文献_第9页

Correction: Fundamental and practical approaches for single-cell ATAC-seq analysis 更正：单细胞 ATAC-seq 分析的基础与实用方法

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-04-01 DOI: 10.1007/s42994-024-00154-8

Peiyu Shi, Yage Nie, Jiawen Yang, Weixing Zhang, Zhongjie Tang, Jin Xu

引用次数: 0

Characterization and functional analysis of gerbera plant defensin (PDF) genes reveal the role of GhPDF2.4 in defense against the root rot pathogen Phytophthora cryptogea 非洲菊植物防御素（PDF）基因的特征和功能分析揭示了 GhPDF2.4 在防御根腐病病原体隐杆线虫（Phytophthora cryptogea）中的作用

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-31 DOI: 10.1007/s42994-024-00146-8

Chunzhen Cheng, Huan Wu, Yongyan Zhang

{"title":"Characterization and functional analysis of gerbera plant defensin (PDF) genes reveal the role of GhPDF2.4 in defense against the root rot pathogen Phytophthora cryptogea","authors":"Chunzhen Cheng, Huan Wu, Yongyan Zhang","doi":"10.1007/s42994-024-00146-8","DOIUrl":"10.1007/s42994-024-00146-8","url":null,"abstract":"<div>Gerbera (Gerbera hybrida), a major fresh cut flower crop, is very susceptible to root rot disease. Although plant defensins (PDFs), a major group of plant antimicrobial peptides, display broad-spectrum antifungal and antibacterial activities, PDF genes in gerbera have not been systematically characterized. Here, we identified and cloned nine PDF genes from gerbera and divided them into two classes based on phylogenetic analysis. Most Class I GhPDF genes were highly expressed in petioles, whereas all Class II GhPDF genes were highly expressed in roots. Phytophthora cryptogea inoculation strongly upregulated all Class II GhPDF genes in roots and upregulated all Class I GhPDF genes in petioles. Transient overexpression of GhPDF1.5 and GhPDF2.4 inhibited P. cryptogea infection in tobacco (Nicotiana benthamiana) leaves. Transient overexpression of GhPDF2.4, but not GhPDF1.5, significantly upregulated ACO and LOX gene expression in tobacco leaves, indicating that overexpressing GhPDF2.4 activated the jasmonic acid/ethylene defense pathway and that the two types of GhPDFs have different modes of action. Prokaryotically expressed recombinant GhPDF2.4 inhibited mycelial growth and delayed the hyphal swelling of P. cryptogea, in vitro, indicating that GhPDF2.4 is a morphogenetic defensin. Moreover, the addition of GhPDF2.4 to plant culture medium alleviated the root rot symptoms of in vitro-grown gerbera seedlings and greatly reduced pathogen titer in P. cryptogea-inoculated gerbera roots in the early stages of treatment. Our study provides a basis for the use of GhPDFs, especially GhPDF2.4, for controlling root rot disease in gerbera.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"325 - 338"},"PeriodicalIF":4.6,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00146-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removal of the C4-domain preserves the drought tolerance enhanced by CsMYB4a and eliminates the negative impact of this transcription factor on plant growth 移除 C4 域可保留 CsMYB4a 增强的耐旱性，并消除该转录因子对植物生长的负面影响

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-28 DOI: 10.1007/s42994-024-00149-5

Mingzhuo Li, Guoliang Ma, Xiu Li, Lili Guo, Yanzhi Li, Yajun Liu, Wenzhao Wang, Xiaolan Jiang, De-Yu Xie, Liping Gao, Tao Xia

{"title":"Removal of the C4-domain preserves the drought tolerance enhanced by CsMYB4a and eliminates the negative impact of this transcription factor on plant growth","authors":"Mingzhuo Li, Guoliang Ma, Xiu Li, Lili Guo, Yanzhi Li, Yajun Liu, Wenzhao Wang, Xiaolan Jiang, De-Yu Xie, Liping Gao, Tao Xia","doi":"10.1007/s42994-024-00149-5","DOIUrl":"10.1007/s42994-024-00149-5","url":null,"abstract":"<div>The MYB4 transcription factor family regulates plant traits. However, their overexpression often results in undesirable side effects like growth reduction. We have reported a green tea (Camellia sinensis) MYB4 transcription factor (CsMYB4) that represses the phenylpropanoid and shikimate pathways and stunts plant growth and development. In the current study, we observed that in CsMYB4a transgenic tobacco (Nicotiana tabacum) plants, primary metabolism was altered, including sugar and amino acid metabolism, which demonstrated a pleiotropic regulation by CsMYB4a. The CsMYB4a transgenic tobacco plants had improved drought tolerance, which correlated to alterations in carbohydrate metabolism and an increase in proline content, as revealed by metabolic profiling and transcriptomic analysis. To mitigate the undesirable repressive side effects on plant traits, including dwarfism, shrunken leaves, and shorter roots of CsMYB4a transgenic plants, we deleted the C4 domain of CsMYB4a to obtain a CsMYB4a-DC4 variant and then overexpressed it in transgenic plants (CsMYB4a-DC4). These CsMYB4a-DC4 plants displayed a normal growth and had improved drought tolerance. Metabolite analysis demonstrated that the contents of carbohydrates and proline were increased in these transgenic plants. Our findings suggest that an approriate modification of TFs can generate novel crop traits, thus providing potential agricultural benefits and expanding its application to various crops.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"368 - 374"},"PeriodicalIF":4.6,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00149-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140369003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations 利用携带 D156R 和 E795L 突变的 LbCas12a 变体提高拟南芥的编辑效率

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-26 DOI: 10.1007/s42994-024-00144-w

Cuiping Xin, Dexin Qiao, Junya Wang, Wei Sun, Zhenghong Cao, Yu Lu, Yuanyuan Jiang, Yiping Chai, Xue-Chen Wang, Qi-jun Chen

{"title":"Enhanced editing efficiency in Arabidopsis with a LbCas12a variant harboring D156R and E795L mutations","authors":"Cuiping Xin, Dexin Qiao, Junya Wang, Wei Sun, Zhenghong Cao, Yu Lu, Yuanyuan Jiang, Yiping Chai, Xue-Chen Wang, Qi-jun Chen","doi":"10.1007/s42994-024-00144-w","DOIUrl":"10.1007/s42994-024-00144-w","url":null,"abstract":"<div>Cas12a (Cpf1), a Class 2 Type V CRISPR/Cas nuclease, has several unique attributes for genome editing and may provide a valuable alternative to Cas9. However, a low editing efficiency due to temperature sensitivity and insufficient cleavage activity of the Cas12a nuclease are major obstacles to its broad application. In this report, we generated two variants, ttAsCas12 Ultra and ttLbCas12a Ultra harboring three (E174R, M537R, and F870L) or two (D156R and E795L) mutations, respectively, by combining the mutations from the temperature-tolerant variants ttAsCas12a (E174R) and ttLbCas12a (D156R), and those from the highly active variants AsCas12a Ultra (M537R and F870L) and LbCas12a Ultra (E795L). We compared editing efficiencies of the five resulting Cas12a variants (LbCas12a, ttLbCas12a, ttLbCas12a Ultra, AsCas12a Ultra, and ttAsCas12 Ultra) at six target sites of four genes in Arabidopsis (Arabidopsis thaliana). The variant ttLbCas12a Ultra, harboring the D156R and E795L mutations, exhibited the highest editing efficiency of all variants tested in Arabidopsis and can be used to generate homozygous or biallelic mutants in a single generation in Arabidopsis plants grown at 22 °C. In addition, optimization of ttLbCas12a Ultra, by varying nuclear localization signal sequences and codon usage, further greatly improved editing efficiency. Collectively, our results indicate that ttLbCas12a Ultra is a valuable alternative to Cas9 for editing genes or promoters in Arabidopsis.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"117 - 126"},"PeriodicalIF":4.6,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00144-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140379325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit 揭示一氧化氮对苦瓜果实采后成熟和衰老的特殊调节作用

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-21 DOI: 10.1007/s42994-023-00110-y

Hongwei Wang, Ling Li, Lili Ma, Alisdair R. Fernie, Anzhen Fu, Chunmei Bai, Zhaoze Sang, Susu Guo, Fan Zhang, Qing Wang, Yanyan Zheng, Jinhua Zuo

{"title":"Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit","authors":"Hongwei Wang, Ling Li, Lili Ma, Alisdair R. Fernie, Anzhen Fu, Chunmei Bai, Zhaoze Sang, Susu Guo, Fan Zhang, Qing Wang, Yanyan Zheng, Jinhua Zuo","doi":"10.1007/s42994-023-00110-y","DOIUrl":"10.1007/s42994-023-00110-y","url":null,"abstract":"<div>Bitter melon fruit is susceptible to yellowing, softening, and rotting under room-temperature storage conditions, resulting in reduced commercial value. Nitric oxide (NO) is an important signaling molecule and plays a crucial role in regulating the fruit postharvest quality. In this study, we investigated the effects of NO treatment on changes in sensory and firmness of bitter melon fruit during postharvest storage. Moreover, transcriptomic, metabolomic, and proteomic analyses were performed to elucidate the regulatory mechanisms through which NO treatment delays the ripening and senescence of bitter melon fruit. Our results show that differentially expressed genes (DEGs) were involved in fruit texture (CSLE, β-Gal, and PME), plant hormone signal transduction (ACS, JAR4, and AUX28), and fruit flavor and aroma (SUS2, LOX, and GDH2). In addition, proteins differentially abundant were associated with fruit texture (PLY, PME, and PGA) and plant hormone signal transduction (PBL15, JAR1, and PYL9). Moreover, NO significantly increased the abundance of key enzymes involved in the phenylpropanoid biosynthetic pathway, thus enhancing the disease resistance and alleviating softening of bitter melon fruit. Finally, differential metabolites mainly included phenolic acids, terpenoids, and flavonoids. These results provide a theoretical basis for further studies on the physiological changes associated with postharvest ripening and senescence of bitter melon fruit.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"29 - 45"},"PeriodicalIF":4.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00110-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140221401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants 将水稻内源 N-甲基嘌呤 DNA 糖基化酶与植物腺嘌呤碱基转换编辑器 ABE8e 融合，可在水稻植株中进行 A-K 碱基编辑

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-21 DOI: 10.1007/s42994-024-00138-8

Yucai Li, Shaoya Li, Chenfei Li, Chen Zhang, Lei Yan, Jingying Li, Yubing He, Yan Guo, Lanqin Xia

{"title":"Fusion of a rice endogenous N-methylpurine DNA glycosylase to a plant adenine base transition editor ABE8e enables A-to-K base editing in rice plants","authors":"Yucai Li, Shaoya Li, Chenfei Li, Chen Zhang, Lei Yan, Jingying Li, Yubing He, Yan Guo, Lanqin Xia","doi":"10.1007/s42994-024-00138-8","DOIUrl":"10.1007/s42994-024-00138-8","url":null,"abstract":"<div>Engineering of a new type of plant base editor for simultaneous adenine transition and transversion within the editing window will greatly expand the scope and potential of base editing in directed evolution and crop improvement. Here, we isolated a rice endogenous hypoxanthine excision protein, N-methylpurine DNA glycosylase (OsMPG), and engineered two plant A-to-K (K = G or T) base editors, rAKBE01 and rAKBE02, for simultaneous adenine transition and transversion base editing in rice by fusing OsMPG or its mutant mOsMPG to a plant adenine transition base editor, ABE8e. We further coupled either OsMPG or mOsMPG with a transactivation factor VP64 to generate rAKBE03 and rAKBE04, respectively. Testing these four rAKBEs, at five endogenous loci in rice protoplasts, indicated that rAKBE03 and rAKBE04 enabled higher levels of A-to-G base transitions when compared to ABE8e and ABE8e-VP64. Furthermore, whereas rAKBE01 only enabled A-to-C/T editing at one endogenous locus, in comparison with rAKBE02 and rAKBE03, rAKBE04 could significantly improve the A-to-C/T base transversion efficiencies by up to 6.57- and 1.75-fold in the rice protoplasts, respectively. Moreover, although no stable lines with A-to-C transversion were induced by rAKBE01 and rAKBE04, rAKBE04 could enable simultaneous A-to-G and A-to-T transition and transversion base editing, at all the five target loci, with the efficiencies of A-to-G transition and A-to-T transversion editing ranging from 70.97 to 92.31% and 1.67 to 4.84% in rice stable lines, respectively. Together, these rAKBEs enable different portfolios of editing products and, thus, now expands the potential of base editing in diverse application scenario for crop improvement.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"127 - 139"},"PeriodicalIF":4.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00138-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140222282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive one-pot ROA assay for rapid miRNA detection 用于快速检测 miRNA 的灵敏的一次性 ROA 分析法

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-18 DOI: 10.1007/s42994-024-00140-0

Zhihao Hou, Wenpeng Deng, Alun Li, Ya Zhang, Jianye Chang, Xinyue Guan, Yuxiao Chang, Kaile Wang, Xinjie Wang, Jue Ruan

{"title":"A sensitive one-pot ROA assay for rapid miRNA detection","authors":"Zhihao Hou, Wenpeng Deng, Alun Li, Ya Zhang, Jianye Chang, Xinyue Guan, Yuxiao Chang, Kaile Wang, Xinjie Wang, Jue Ruan","doi":"10.1007/s42994-024-00140-0","DOIUrl":"10.1007/s42994-024-00140-0","url":null,"abstract":"<div>MicroRNAs (miRNAs) and short RNA fragments (18–25 nt) are crucial biomarkers in biological research and disease diagnostics. However, their accurate and rapid detection remains a challenge, largely due to their low abundance, short length, and sequence similarities. In this study, we report on a highly sensitive, one-step RNA O-circle amplification (ROA) assay for rapid and accurate miRNA detection. The ROA assay commences with the hybridization of a circular probe with the test RNA, followed by a linear rolling circle amplification (RCA) using dUTP. This amplification process is facilitated by U-nick reactions, which lead to an exponential amplification for readout. Under optimized conditions, assays can be completed within an hour, producing an amplification yield up to the microgram level, with a detection limit as low as 0.15 fmol (6 pM). Notably, the ROA assay requires only one step, and the results can be easily read visually, making it user-friendly. This ROA assay has proven effective in detecting various miRNAs and phage ssRNA. Overall, the ROA assay offers a user-friendly, rapid, and accurate solution for miRNA detection.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"298 - 308"},"PeriodicalIF":4.6,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00140-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140233780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RUBY reporter for visual selection in soybean genome editing 用于大豆基因组编辑视觉选择的 RUBY 报告器

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-18 DOI: 10.1007/s42994-024-00148-6

Li Chen, Yupeng Cai, Xiaoqian Liu, Weiwei Yao, Shuiqing Wu, Wensheng Hou

{"title":"The RUBY reporter for visual selection in soybean genome editing","authors":"Li Chen, Yupeng Cai, Xiaoqian Liu, Weiwei Yao, Shuiqing Wu, Wensheng Hou","doi":"10.1007/s42994-024-00148-6","DOIUrl":"10.1007/s42994-024-00148-6","url":null,"abstract":"<div>Current systems to screen for transgenic soybeans (Glycine max) involve laborious molecular assays or the expression of fluorescent markers that are difficult to see in soybean plants. Therefore, a visual system for early screening of transgenic plants would increase the efficiency of crop improvement by genome editing. The RUBY reporter system, which consists of three genes encoding betalain biosynthetic enzymes, leading to the accumulation of purple pigment in transgenic tissue, has been employed in some plants and dikaryon fungi. Here, we assessed the RUBY reporter for visual verification during soybean transformation. We show that RUBY can be expressed in soybean, allowing for visual confirmation of transgenic events without the need for specialized equipment. Plants with visible accumulation of purple pigment in any tissue were successfully transformed, confirming the accuracy of the RUBY system as a visual indicator. We also assessed the genetic stability of the transgene across generations, which can be performed very early, using the cotyledons of the progeny. Transgene-free seedlings have a distinct green color, facilitating the selection of genome-edited but transgene-free soybean seedlings for harvest. Using the RUBY system, we quickly identified a transgene-free Gmwaxy mutant in the T1 generation. This system thus provides an efficient and convenient tool for soybean genome editing.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"209 - 213"},"PeriodicalIF":4.6,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140231507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution in Arabidopsis 急性热胁迫对拟南芥线粒体功能、超微结构和心磷脂分布的影响

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-15 DOI: 10.1007/s42994-024-00151-x

Yukang Wang, Ronghui Pan, Jianping Hu

{"title":"Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution in Arabidopsis","authors":"Yukang Wang, Ronghui Pan, Jianping Hu","doi":"10.1007/s42994-024-00151-x","DOIUrl":"10.1007/s42994-024-00151-x","url":null,"abstract":"<div>Besides providing energy to sustain life, mitochondria also play crucial roles in stress response and programmed cell death. The mitochondrial hallmark lipid, cardiolipin (CL), is essential to the maintenance of mitochondrial structure and function. However, how mitochondria and CL are involved in stress response is not as well defined in plants as in animal and yeast cells. We previously revealed a role for CL in mitochondrial fission and in heat stress response in Arabidopsis. To further determine the involvement of mitochondria and CL in plant heat response, here we treated Arabidopsis seedlings with varied lengths of acute heat stress. These treatments resulted in decreases in mitochondrial membrane potential, disruption of mitochondrial ultrastructure, accumulation of mitochondrial reactive-oxygen species (ROS), and redistribution of CL to the outer mitochondrial membrane and to a novel type of vesicle. The level of the observed changes correlated with the severeness of the heat stress, indicating the strong relevance of these processes to stress response. Our findings provide the basis for studying mechanisms underpinning the role of mitochondria and CL in plant stress response.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"362 - 367"},"PeriodicalIF":4.6,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00151-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The maize ZmCPK39-ZmKnox2 module regulates plant height 玉米 ZmCPK39-ZmKnox2 模块调控植株高度

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2024-03-15 DOI: 10.1007/s42994-024-00150-y

Mang Zhu, Chenyu Guo, Xiaohui Zhang, Yulin Liu, Xiaohui Jiang, Limei Chen, Mingliang Xu

{"title":"The maize ZmCPK39-ZmKnox2 module regulates plant height","authors":"Mang Zhu, Chenyu Guo, Xiaohui Zhang, Yulin Liu, Xiaohui Jiang, Limei Chen, Mingliang Xu","doi":"10.1007/s42994-024-00150-y","DOIUrl":"10.1007/s42994-024-00150-y","url":null,"abstract":"<div>Plant height is an important agronomic trait that affects high-density tolerance and lodging resistance. However, the regulators and their underlying molecular mechanisms controlling plant height in maize remain understudied. Here, we report that knockout mutants of the calcium-dependent protein kinase gene ZmCPK39 (ZmCPK39-KO) exhibit dramatically reduced plant height, characterized by shorter internodes and a slight decrease in node numbers. Furthermore, we identified a ZmCPK39-interacting protein, the knotted-related homeobox (ZmKnox2), and observed that plant height was also significantly reduced in a mutator transposon-inserted mutant of ZmKnox2 (ZmKnox2-Mu). Combined analysis of transcriptomic and metabonomic data indicates that multiple phytohormone signaling and photosynthesis pathways are disrupted in both ZmCPK39-KO and ZmKnox2-Mu mutants. Taken together, these results provide new insights into the function of ZmCPK39 and identify potential targets for breeding lodging-resistant and high-density tolerant maize cultivars.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"356 - 361"},"PeriodicalIF":4.6,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00150-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0