aBIOTECH最新文献

{"title":"Correction: Co-expression of GR79 EPSPS and GAT generates high glyphosate-resistant alfalfa with low glyphosate residues","authors":"Yingying Meng, Wenwen Zhang, Zhaoming Wang, Feng Yuan, Sandui Guo, Hao Lin, Lifang Niu","doi":"10.1007/s42994-023-00135-3","DOIUrl":"10.1007/s42994-023-00135-3","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"116 - 116"},"PeriodicalIF":4.6,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00135-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140488294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection of Cas9-free progeny in Arabidopsis","authors":"Feng-Zhu Wang,&nbsp;Ying Bao,&nbsp;Zhenxiang Li,&nbsp;Xiangyu Xiong,&nbsp;Jian-Feng Li","doi":"10.1007/s42994-023-00132-6","DOIUrl":"10.1007/s42994-023-00132-6","url":null,"abstract":"<div><p>The CRISPR/Cas9 technology revolutionizes targeted gene knockout in diverse organisms including plants. However, screening edited alleles, particularly those with multiplex editing, from herbicide- or antibiotic-resistant transgenic plants and segregating out the <i>Cas9</i> transgene represent two laborious processes. Current solutions to facilitate these processes rely on different selection markers. Here, by taking advantage of the opposite functions of a <span>d</span>-amino acid oxidase (DAO) in detoxifying <span>d</span>-serine and in metabolizing non-toxic <span>d</span>-valine to a cytotoxic product, we develop a DAO-based selection system that simultaneously enables the enrichment of multigene edited alleles and elimination of <i>Cas9</i>-containing progeny in <i>Arabidopsis thaliana</i>. Among five DAOs tested in <i>Escherichia coli</i>, the one encoded by <i>Trigonopsis variabilis</i> (TvDAO) could confer slightly stronger <span>d</span>-serine resistance than other homologs. Transgenic expression of <i>TvDAO</i> in <i>Arabidopsis</i> allowed a clear distinction between transgenic and non-transgenic plants in both <span>d</span>-serine-conditioned positive selection and <span>d</span>-valine-conditioned negative selection. As a proof of concept, we combined CRISPR-induced single-strand annealing repair of a dead <i>TvDAO</i> with <span>d</span>-serine-based positive selection to help identify transgenic plants with multiplex editing, where <span>d</span>-serine-resistant plants exhibited considerably higher co-editing frequencies at three endogenous target genes than those selected by hygromycin. Subsequently, <span>d</span>-valine-based negative selection successfully removed <i>Cas9</i> and <i>TvDAO</i> transgenes from the survival offspring carrying inherited mutations. Collectively, this work provides a novel strategy to ease CRISPR mutant identification and <i>Cas9</i> transgene elimination using a single selection marker, which promises more efficient and simplified multiplex CRISPR editing in plants.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"140 - 150"},"PeriodicalIF":4.6,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00132-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139523263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Integration of light and hormone signaling pathways in the regulation of plant shade avoidance syndrome","authors":"Yang Liu,&nbsp;Fereshteh Jafari,&nbsp;Haiyang Wang","doi":"10.1007/s42994-023-00136-2","DOIUrl":"10.1007/s42994-023-00136-2","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"115 - 115"},"PeriodicalIF":4.6,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00136-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139523303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of gene-edited plants in Europe: from the valley of tears into the shining sun?","authors":"Holger Puchta","doi":"10.1007/s42994-023-00130-8","DOIUrl":"10.1007/s42994-023-00130-8","url":null,"abstract":"<div><p>Some 20 years ago, the EU introduced complex regulatory rules for the growth of transgenic crops, which resulted in a de facto ban to grow these plants in fields within most European countries. With the rise of novel genome editing technologies, it has become possible to improve crops genetically in a directed way without the need for incorporation of foreign genes. Unfortunately, in 2018, the European Court of Justice ruled that such gene-edited plants are to be regulated like transgenic plants. Since then, European scientists and breeders have challenged this decision and requested a revision of this outdated law. Finally, after 5 years, the European Commission has now published a proposal on how, in the future, to regulate crops produced by new breeding technologies. The proposal tries to find a balance between the different interest groups in Europe. On one side, genetically modified plants, which cannot be discerned from their natural counterparts, will exclusively be used for food and feed and are—besides a registration step—not to be regulated at all. On the other side, plants expressing herbicide resistance are to be excluded from this regulation, a concession to the strong environmental associations and NGOs in Europe. Moreover, edited crops are to be excluded from organic farming to protect the business interests of the strong organic sector in Europe. Nevertheless, if this law passes European parliament and council, unchanged, it will present a big step forward toward establishing a more sustainable European agricultural system. Thus, it might soon be possible to develop and grow crops that are more adapted to global warming and whose cultivation will require lower amounts of pesticides. However, there is still a long way to go until the law is passed. Too often, the storm of arguments raised by the opponents, based on irrational fears of mutations and a naive understanding of nature, has fallen on fruitful ground in Europe.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 2","pages":"231 - 238"},"PeriodicalIF":4.6,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00130-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two H3K36 methyltransferases differentially associate with transcriptional activity and enrichment of facultative heterochromatin in rice blast fungus","authors":"Mengting Xu,&nbsp;Ziyue Sun,&nbsp;Huanbin Shi,&nbsp;Jiangnan Yue,&nbsp;Xiaohui Xiong,&nbsp;Zhongling Wu,&nbsp;Yanjun Kou,&nbsp;Zeng Tao","doi":"10.1007/s42994-023-00127-3","DOIUrl":"10.1007/s42994-023-00127-3","url":null,"abstract":"<div><p>Di- and tri-methylation of lysine 36 on histone H3 (H3K36me2/3) is catalysed by histone methyltransferase Set2, which plays an essential role in transcriptional regulation. Although there is a single H3K36 methyltransferase in yeast and higher eukaryotes, two H3K36 methyltransferases, Ash1 and Set2, were present in many filamentous fungi. However, their roles in H3K36 methylation and transcriptional regulation remained unclear. Combined with methods of RNA-seq and ChIP-seq, we revealed that both Ash1 and Set2 are redundantly required for the full H3K36me2/3 activity in <i>Magnaporthe oryzae</i>, which causes the devastating worldwide rice blast disease. Ash1 and Set2 distinguish genomic H3K36me2/3-marked regions and are differentially associated with repressed and activated transcription, respectively. Furthermore, Ash1-catalysed H3K36me2 was co-localized with H3K27me3 at the chromatin, and Ash1 was required for the enrichment and transcriptional silencing of H3K27me3-occupied genes. With the different roles of Ash1 and Set2, in H3K36me2/3 enrichment and transcriptional regulation on the stress-responsive genes, they differentially respond to various stresses in <i>M. oryzae</i>. Overall, we reveal a novel mechanism by which two H3K36 methyltransferases catalyze H3K36me2/3 that differentially associate with transcriptional activities and contribute to enrichment of facultative heterochromatin in eukaryotes.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"1 - 16"},"PeriodicalIF":4.6,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00127-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Publisher Correction: The RNA-binding domain of DCL3 is required for long-distance RNAi signaling","authors":"Jie Li,&nbsp;Bo-Sen Zhang,&nbsp;Hua-Wei Wu,&nbsp;Cheng-Lan Liu,&nbsp;Hui-Shan Guo,&nbsp;Jian-Hua Zhao","doi":"10.1007/s42994-023-00128-2","DOIUrl":"10.1007/s42994-023-00128-2","url":null,"abstract":"","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"114 - 114"},"PeriodicalIF":4.6,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10987431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mining salt stress-related genes in Spartina alterniflora via analyzing co-evolution signal across 365 plant species using phylogenetic profiling","authors":"Shang Gao,&nbsp;Shoukun Chen,&nbsp;Maogeng Yang,&nbsp;Jinran Wu,&nbsp;Shihua Chen,&nbsp;Huihui Li","doi":"10.1007/s42994-023-00125-5","DOIUrl":"10.1007/s42994-023-00125-5","url":null,"abstract":"<div><p>With the increasing number of sequenced species, phylogenetic profiling (PP) has become a powerful method to predict functional genes based on co-evolutionary information. However, its potential in plant genomics has not yet been fully explored. In this context, we combined the power of machine learning and PP to identify salt stress-related genes in a halophytic grass, <i>Spartina alterniflora</i>, using evolutionary information generated from 365 plant species. Our results showed that the genes highly co-evolved with known salt stress-related genes are enriched in biological processes of ion transport, detoxification and metabolic pathways. For ion transport, five identified genes coding two sodium and three potassium transporters were validated to be able to uptake Na⁺. In addition, we identified two orthologs of trichome-related AtR3-MYB genes, <i>SaCPC1</i> and <i>SaCPC2</i>, which may be involved in salinity responses. Genes co-evolved with <i>SaCPCs</i> were enriched in functions related to the circadian rhythm and abiotic stress responses. Overall, this work demonstrates the feasibility of mining salt stress-related genes using evolutionary information, highlighting the potential of PP as a valuable tool for plant functional genomics.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 4","pages":"291 - 302"},"PeriodicalIF":4.6,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00125-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138593356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting rice diseases using advanced technologies at different scales: present status and future perspectives","authors":"Ruyue Li,&nbsp;Sishi Chen,&nbsp;Haruna Matsumoto,&nbsp;Mostafa Gouda,&nbsp;Yusufjon Gafforov,&nbsp;Mengcen Wang,&nbsp;Yufei Liu","doi":"10.1007/s42994-023-00126-4","DOIUrl":"10.1007/s42994-023-00126-4","url":null,"abstract":"<div><p>The past few years have witnessed significant progress in emerging disease detection techniques for accurately and rapidly tracking rice diseases and predicting potential solutions. In this review we focus on image processing techniques using machine learning (ML) and deep learning (DL) models related to multi-scale rice diseases. Furthermore, we summarize applications of different detection techniques, including genomic, physiological, and biochemical approaches. In addition, we also present the state-of-the-art in contemporary optical sensing applications of pathogen–plant interaction phenotypes. This review serves as a valuable resource for researchers seeking effective solutions to address the challenges of high-throughput data and model recognition for early detection of issues affecting rice crops through ML and DL models.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 4","pages":"359 - 371"},"PeriodicalIF":4.6,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The RNA-binding domain of DCL3 is required for long-distance RNAi signaling","authors":"Jie Li,&nbsp;Bo-Sen Zhang,&nbsp;Hua-Wei Wu,&nbsp;Cheng-Lan Liu,&nbsp;Hui-Shan Guo,&nbsp;Jian-Hua Zhao","doi":"10.1007/s42994-023-00124-6","DOIUrl":"10.1007/s42994-023-00124-6","url":null,"abstract":"<div><p>Small RNA (sRNA)-mediated RNA silencing (also known as RNA interference, or RNAi) is a conserved mechanism in eukaryotes that includes RNA degradation, DNA methylation, heterochromatin formation and protein translation repression. In plants, sRNAs can move either cell-to-cell or systemically, thereby acting as mobile silencing signals to trigger noncell autonomous silencing. However, whether and what proteins are also involved in noncell autonomous silencing have not been elucidated. In this study, we utilized a previously reported inducible RNAi plant, <i>PDSi</i>, which can induce systemic silencing of the endogenous <i>PDS</i> gene, and we demonstrated that DCL3 is involved in systemic <i>PDS</i> silencing through its RNA binding activity. We confirmed that the C-terminus of DCL3, including the predicted RNA-binding domain, is capable of binding short RNAs. Mutations affecting RNA binding, but not processing activity, reduced systemic <i>PDS</i> silencing, indicating that DCL3 binding to RNAs is required for the induction of systemic silencing. Cucumber mosaic virus infection assays showed that the RNA-binding activity of DCL3 is required for antiviral RNAi in systemically noninoculated leaves. Our findings demonstrate that DCL3 acts as a signaling agent involved in noncell autonomous silencing and an antiviral effect in addition to its previously known function in the generation of 24-nucleotide sRNAs.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 1","pages":"17 - 28"},"PeriodicalIF":4.6,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10987413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of seed traits in soybean","authors":"Yang Hu,&nbsp;Yue Liu,&nbsp;Jun-Jie Wei,&nbsp;Wan-Ke Zhang,&nbsp;Shou-Yi Chen,&nbsp;Jin-Song Zhang","doi":"10.1007/s42994-023-00122-8","DOIUrl":"10.1007/s42994-023-00122-8","url":null,"abstract":"<div><p>Soybean (<i>Glycine max</i>) is an essential economic crop that provides vegetative oil and protein for humans, worldwide. Increasing soybean yield as well as improving seed quality is of great importance. Seed weight/size, oil and protein content are the three major traits determining seed quality, and seed weight also influences soybean yield. In recent years, the availability of soybean omics data and the development of related techniques have paved the way for better research on soybean functional genomics, providing a comprehensive understanding of gene functions. This review summarizes the regulatory genes that influence seed size/weight, oil content and protein content in soybean. We also provided a general overview of the pleiotropic effect for the genes in controlling seed traits and environmental stresses. Ultimately, it is expected that this review will be beneficial in breeding improved traits in soybean.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 4","pages":"372 - 385"},"PeriodicalIF":4.6,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}