aBIOTECH最新文献_第9页

In maize, co-expression of GAT and GR79-EPSPS provides high glyphosate resistance, along with low glyphosate residues 在玉米中，GAT 和 GR79-EPSPS 的共同表达提供了高草甘膦抗性和低草甘膦残留。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-09-01 DOI: 10.1007/s42994-023-00114-8

Shengyan Li, Pengcheng Li, Xiangyin Li, Ning Wen, Yinxiao Wang, Wei Lu, Min Lin, Zhihong Lang

{"title":"In maize, co-expression of GAT and GR79-EPSPS provides high glyphosate resistance, along with low glyphosate residues","authors":"Shengyan Li, Pengcheng Li, Xiangyin Li, Ning Wen, Yinxiao Wang, Wei Lu, Min Lin, Zhihong Lang","doi":"10.1007/s42994-023-00114-8","DOIUrl":"10.1007/s42994-023-00114-8","url":null,"abstract":"<div>Herbicide tolerance has been the dominant trait introduced during the global commercialization of genetically modified (GM) crops. Herbicide-tolerant crops, especially glyphosate-resistant crops, offer great advantages for weed management; however, despite these benefits, glyphosate-resistant maize (Zea mays L.) has not yet been commercially deployed in China. To develop a new bio-breeding resource for glyphosate-resistant maize, we introduced a codon-optimized glyphosate N-acetyltransferase gene, gat, and the enolpyruvyl-shikimate-3-phosphate synthase gene, gr79-epsps, into the maize variety B104. We selected a genetically stable high glyphosate resistance (GR) transgenic event, designated GG2, from the transgenic maize population through screening with high doses of glyphosate. A molecular analysis demonstrated that single copy of gat and gr79-epsps were integrated into the maize genome, and these two genes were stably transcribed and translated. Field trials showed that the transgenic event GG2 could tolerate 9000 g acid equivalent (a.e.) glyphosate per ha with no effect on phenotype or yield. A gas chromatography-mass spectrometry (GC–MS) analysis revealed that, shortly after glyphosate application, the glyphosate (PMG) and aminomethylphosphonic acid (AMPA) residues in GG2 leaves decreased by more than 90% compared to their levels in HGK60 transgenic plants, which only harbored the epsps gene. Additionally, PMG and its metabolic residues (AMPA and N-acetyl-PMG) were not detected in the silage or seeds of GG2, even when far more than the recommended agricultural dose of glyphosate was applied. The co-expression of gat and gr79-epsps, therefore, confers GG2 with high GR and a low risk of herbicide residue accumulation, making this germplasm a valuable GR event in herbicide-tolerant maize breeding.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 4","pages":"277 - 290"},"PeriodicalIF":4.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81852192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing nanobodies to expand the recognition spectrum of plant NLRs for diverse pathogens 利用纳米体扩展植物NLRs对不同病原体的识别光谱

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-08-16 DOI: 10.1007/s42994-023-00111-x

Ruyi Wang, Guo-Liang Wang, Yuese Ning

引用次数: 0

Unlikely heroes on the long and winding road to potato inbreeding 在漫长而曲折的马铃薯近亲繁殖道路上，不太可能成为英雄。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-07-18 DOI: 10.1007/s42994-023-00109-5

Luca Comai

引用次数: 0

Knockout of a gene encoding a Gγ protein boosts alkaline tolerance in cereal crops 敲除一个编码Gγ蛋白的基因可以提高谷物的耐碱性。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-07-02 DOI: 10.1007/s42994-023-00106-8

Peitong Wang, Jian Feng Ma

引用次数: 0

The transcription factor HBF1 directly activates expression of multiple flowering time repressors to delay rice flowering 转录因子HBF1直接激活多个开花时间抑制因子的表达，从而延迟水稻开花。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-06-30 DOI: 10.1007/s42994-023-00107-7

Cong Li, Liya Zhang, Xin Wang, Chunsheng Yu, Tao Zhao, Bin Liu, Hongyu Li, Jun Liu, Chunyu Zhang

{"title":"The transcription factor HBF1 directly activates expression of multiple flowering time repressors to delay rice flowering","authors":"Cong Li, Liya Zhang, Xin Wang, Chunsheng Yu, Tao Zhao, Bin Liu, Hongyu Li, Jun Liu, Chunyu Zhang","doi":"10.1007/s42994-023-00107-7","DOIUrl":"10.1007/s42994-023-00107-7","url":null,"abstract":"<div>Flowering time (or heading date) is an important agronomic trait that determines the environmental adaptability and yield of many crops, including rice (Oryza sativa L.). Hd3a BINDING REPRESSOR FACTOR 1 (HBF1), a basic leucine zipper transcription factor, delays flowering by decreasing the expression of Early heading date 1 (Ehd1), Heading date 3a (Hd3a), and RICE FLOWERING LOCUS T 1 (RFT1), but the underlying molecular mechanisms have not been fully elucidated. Here, we employed the hybrid transcriptional factor (HTF) strategy to enhance the transcriptional activity of HBF1 by fusing it to four copies of the activation domain from Herpes simplex virus VP16. We discovered that transgenic rice lines overexpressing HBF1-VP64 (HBF1V) show significant delays in time to flower, compared to lines overexpressing HBF1-MYC or wild-type plants, via the Ehd1–Hd3a/RFT1 pathway, under both long-day and short-day conditions. Transcriptome deep sequencing analysis indicated that 19 WRKY family genes are upregulated in the HBF1V overexpression line. We demonstrate that the previously unknown gene, OsWRKY64, is a direct downstream target of HBF1 and represses flowering in rice, whereas three known flowering repressor genes, Days to heading 7 (DTH7), CONSTANS 3 (OsCO3), and OsWRKY104, are also direct target genes of HBF1 in flowering regulation. Taking these results together, we propose detailed molecular mechanisms by which HBF1 regulates the time to flower in rice.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 3","pages":"213 - 223"},"PeriodicalIF":3.6,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74073906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment 甲基磺酸乙酯处理产生大麦小孢子纯合突变体群体

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-06-28 DOI: 10.1007/s42994-023-00108-6

Linli Huang, Guangqi Gao, Congcong Jiang, Guimei Guo, Qiang He, Yingjie Zong, Chenghong Liu, Ping Yang

{"title":"Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment","authors":"Linli Huang, Guangqi Gao, Congcong Jiang, Guimei Guo, Qiang He, Yingjie Zong, Chenghong Liu, Ping Yang","doi":"10.1007/s42994-023-00108-6","DOIUrl":"10.1007/s42994-023-00108-6","url":null,"abstract":"<div>Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis are usually heterozygous during the early generations. However, mutations must be fixed prior to phenotyping or field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method to produce double-haploid (DH) plants that are fixed homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced mutagenesis of microspore cultures of barley (Hordeum vulgare) cultivar ‘Hua30’ and landrace ‘HTX’. The EMS concentrations were negatively correlated with the efficiency of callus induction and the frequency of mutant plant regeneration. The two genotypes showed different regeneration efficiencies. The phenotypic variation of the regenerated M1 plants and the presence of genome-wide nucleotide mutations, revealed by whole-genome sequencing, highlight the utility of EMS-induced mutagenesis of isolated microspore cultures for developing DH mutants. Genome-wide analysis of the mutation frequency in the regenerated plants revealed that a considerable proportion of mutations resulted from microspore culture (somaclonal variation) rather than EMS-induced mutagenesis. In addition to producing a population of 1972 homozygous mutant lines that are available for future field trials, this study lays the foundation for optimizing the regeneration efficiency of DH plants and the richness of mutations (mainly by fine-tuning the mutagen dosage).</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 3","pages":"202 - 212"},"PeriodicalIF":3.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00108-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120503570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomic insights into the response of Arabidopsis sepals to mild heat stress 脂质组学研究拟南芥萼片对轻度热胁迫的反应。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-06-06 DOI: 10.1007/s42994-023-00103-x

Zican Chen, Weronika Jasinska, Muhammad Ashraf, Leah Rosental, Jung Hong, Dabing Zhang, Yariv Brotman, Jianxin Shi

{"title":"Lipidomic insights into the response of Arabidopsis sepals to mild heat stress","authors":"Zican Chen, Weronika Jasinska, Muhammad Ashraf, Leah Rosental, Jung Hong, Dabing Zhang, Yariv Brotman, Jianxin Shi","doi":"10.1007/s42994-023-00103-x","DOIUrl":"10.1007/s42994-023-00103-x","url":null,"abstract":"<div>Arabidopsis sepals coordinate flower opening in the morning as ambient temperature rises; however, the underlying molecular mechanisms are poorly understood. Mutation of one heat shock protein encoding gene, HSP70-16, impaired sepal heat stress responses (HSR), disrupting lipid metabolism, especially sepal cuticular lipids, leading to abnormal flower opening. To further explore, to what extent, lipids play roles in this process, in this study, we compared lipidomic changes in sepals of hsp70-16 and vdac3 (mutant of a voltage-dependent anion channel, VDAC3, an HSP70-16 interactor) grown under both normal (22 °C) and mild heat stress (27 °C, mild HS) temperatures. Under normal temperature, neither hsp70-16 nor vdac3 sepals showed significant changes in total lipids; however, vdac3 but not hsp70-16 sepals exhibited significant reductions in the ratios of all detected 11 lipid classes, except the monogalactosyldiacylglycerols (MGDGs). Under mild HS temperature, hsp70-16 but not vdac3 sepals showed dramatic reduction in total lipids. In addition, vdac3 sepals exhibited a significant accumulation of plastidic lipids, especially sulfoquinovosyldiacylglycerols (SQDGs) and phosphatidylglycerols (PGs), whereas hsp70-16 sepals had a significant accumulation of triacylglycerols (TAGs) and simultaneous dramatic reductions in SQDGs and phospholipids (PLs), such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and phosphatidylserines (PSs). These findings revealed that the impact of mild HS on sepal lipidome is influenced by genetic factors, and further, that HSP70-16 and VDAC3 differently affect sepal lipidomic responses to mild HS. Our studies provide a lipidomic insight into functions of HSP and VDAC proteins in the plant’s HSR, in the context of floral development.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 3","pages":"224 - 237"},"PeriodicalIF":3.6,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72672549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Insights into pollen–stigma recognition: self-incompatibility mechanisms serve as interspecies barriers in Brassicaceae? 花粉柱头识别的见解：自交不亲和机制是十字花科种间屏障吗？

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-06-05 DOI: 10.1007/s42994-023-00105-9

Zijun Lan, Sheng Zhong, Li-Jia Qu

引用次数: 1

TIR enzymatic functions: signaling molecules and receptor mechanisms TIR酶功能：信号分子和受体机制。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-05-07 DOI: 10.1007/s42994-023-00104-w

Li Wan

引用次数: 0

A complete MAP kinase cascade controls hyphopodium formation and virulence of Verticillium dahliae 一个完整的MAP激酶级联控制大丽花黄萎病菌的hyphodium形成和毒力

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-05-02 DOI: 10.1007/s42994-023-00102-y

Ziqin Ye, Jun Qin, Yu Wang, Jinghan Zhang, Xiaoyun Wu, Xiangguo Li, Lifan Sun, Jie Zhang

{"title":"A complete MAP kinase cascade controls hyphopodium formation and virulence of Verticillium dahliae","authors":"Ziqin Ye, Jun Qin, Yu Wang, Jinghan Zhang, Xiaoyun Wu, Xiangguo Li, Lifan Sun, Jie Zhang","doi":"10.1007/s42994-023-00102-y","DOIUrl":"10.1007/s42994-023-00102-y","url":null,"abstract":"<div>Phytopathogens develop specialized infection-related structures to penetrate plant cells during infection. Different from phytopathogens that form appressoria or haustoria, the soil-borne root-infecting fungal pathogen Verticillium dahliae forms hyphopodia during infection, which further differentiate into penetration pegs to promote infection. The molecular mechanisms underlying the regulation of hyphopodium formation in V. dahliae remain poorly characterized. Mitogen-activated protein kinases (MAPKs) are highly conserved cytoplasmic kinases that regulate diverse biological processes in eukaryotes. Here we found that deletion of VdKss1, out of the five MAPKs encoded by V. dahliae, significantly impaired V. dahliae hyphopodium formation, in vitro penetration, and pathogenicity in cotton plants. Constitutive activation of MAPK kinase (MAPKK) VdSte7 and MAPK kinase kinase (MAPKKK) VdSte11 specifically activate VdKss1. Deletion of VdSte7 or VdSte11 resulted in a phenotype similar to that of the mutant with VdKss1 deletion. Thus, this study demonstrates that VdSte11-VdSte7-VdKss1 is a core MAPK cascade that regulates hyphopodium formation and pathogenicity in V. dahliae.</div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"4 2","pages":"97 - 107"},"PeriodicalIF":3.6,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00102-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50441384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0