aBIOTECH最新文献_第8页

A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners 基于LexA的酵母双杂交系统用于研究光敏色素与其相互作用伙伴的光可切换相互作用

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2021-02-24 DOI: 10.1007/s42994-021-00034-5

Hong Li, Xinyan Qin, Pengyu Song, Run Han, Jigang Li

{"title":"A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners","authors":"Hong Li, Xinyan Qin, Pengyu Song, Run Han, Jigang Li","doi":"10.1007/s42994-021-00034-5","DOIUrl":"10.1007/s42994-021-00034-5","url":null,"abstract":"<div><p>Phytochromes are a family of photoreceptors in plants that perceive the red (R) and far-red (FR) components of their light environment. Phytochromes exist in vivo in two forms, the inactive Pr form and the active Pfr form, that are interconvertible by treatments with R or FR light. It is believed that phytochromes transduce light signals by interacting with their signaling partners. A GAL4-based light-switchable yeast two-hybrid (Y2H) system was developed two decades ago and has been successfully employed in many studies to determine phytochrome interactions with their signaling components. However, several pairs of interactions between phytochromes and their interactors, such as the phyA-COP1 and phyA-TZP interactions, were demonstrated by other assay systems but were not detected by this GAL4 Y2H system. Here, we report a modified LexA Y2H system, in which the LexA DNA-binding domain is fused to the C-terminus of a phytochrome protein. The conformational changes of phytochromes in response to R and FR light are achieved in yeast cells by exogenously supplying phycocyanobilin (PCB) extracted from <i>Spirulina</i>. The well-defined interaction pairs, including phyA-FHY1 and phyB-PIFs, are well reproducible in this system. Moreover, we show that our system is successful in detecting the phyA-COP1 and phyA-TZP interactions. Together, our study provides an alternative Y2H system that is highly sensitive and reproducible for detecting light-switchable interactions of phytochromes with their interacting partners.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"2 2","pages":"105 - 116"},"PeriodicalIF":3.6,"publicationDate":"2021-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-021-00034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9093382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cell size: a key determinant of meristematic potential in plant protoplasts 细胞大小：植物原生质体分生组织潜能的关键决定因素

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2021-01-01 DOI: 10.1007/s42994-020-00033-y

Ipsita Pujari, Abitha Thomas, Padmalatha S. Rai, Kapaettu Satyamoorthy, Vidhu Sankar Babu

{"title":"Cell size: a key determinant of meristematic potential in plant protoplasts","authors":"Ipsita Pujari, Abitha Thomas, Padmalatha S. Rai, Kapaettu Satyamoorthy, Vidhu Sankar Babu","doi":"10.1007/s42994-020-00033-y","DOIUrl":"10.1007/s42994-020-00033-y","url":null,"abstract":"<div><p>Metabolic pathway reconstruction and gene edits for native natural product synthesis in single plant cells are considered to be less complicated when compared to the production of non-native metabolites. Being an efficient eukaryotic system, plants encompass suitable post-translational modifications. However, slow cell division rate and heterogeneous nature is an impediment for consistent product retrieval from plant cells. Plant cell synchrony can be attained in cultures developed in vitro. Isolated plant protoplasts capable of division, can potentially enhance the unimpaired yield of target bioactives, similar to microbes and unicellular eukaryotes. Evidence from yeast experiments suggests that ‘critical cell size’ and division rates for enhancement machinery, primarily depend on culture conditions and nutrient availability. The cell size control mechanisms in <i>Arabidopsis</i> shoot apical meristem is analogous to yeast notably, fission yeast. If protoplasts isolated from plants are subjected to cell size studies and cell cycle progression in culture, it will answer the underlying molecular mechanisms such as, unicellular to multicellular transition states, longevity, senescence, ‘cell-size resetting’ during organogenesis, and adaptation to external cues.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"2 1","pages":"96 - 104"},"PeriodicalIF":3.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00033-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9088385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome recoding strategies to improve cellular properties: mechanisms and advances 改善细胞特性的基因组编码策略：机制和进展

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-11-19 DOI: 10.1007/s42994-020-00030-1

Tanya Singh, Sudesh Kumar Yadav, Alexander Vainstein, Vinay Kumar

{"title":"Genome recoding strategies to improve cellular properties: mechanisms and advances","authors":"Tanya Singh, Sudesh Kumar Yadav, Alexander Vainstein, Vinay Kumar","doi":"10.1007/s42994-020-00030-1","DOIUrl":"10.1007/s42994-020-00030-1","url":null,"abstract":"<div><p>The genetic code, once believed to be universal and immutable, is now known to contain many variations and is not quite universal. The basis for genome recoding strategy is genetic code variation that can be harnessed to improve cellular properties. Thus, genome recoding is a promising strategy for the enhancement of genome flexibility, allowing for novel functions that are not commonly documented in the organism in its natural environment. Here, the basic concept of genetic code and associated mechanisms for the generation of genetic codon variants, including biased codon usage, codon reassignment, and ambiguous decoding, are extensively discussed. Knowledge of the concept of natural genetic code expansion is also detailed. The generation of recoded organisms and associated mechanisms with basic targeting components, including aminoacyl-tRNA synthetase–tRNA pairs, elongation factor EF-Tu and ribosomes, are highlighted for a comprehensive understanding of this concept. The research associated with the generation of diverse recoded organisms is also discussed. The success of genome recoding in diverse multicellular organisms offers a platform for expanding protein chemistry at the biochemical level with non-canonical amino acids, genetically isolating the synthetic organisms from the natural ones, and fighting viruses, including SARS-<i>CoV</i>2, through the creation of attenuated viruses. In conclusion, genome recoding can offer diverse applications for improving cellular properties in the genome-recoded organisms.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"2 1","pages":"79 - 95"},"PeriodicalIF":3.6,"publicationDate":"2020-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00030-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9147518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Gene editing applications to modulate crop flowering time and seed dormancy 基因编辑在调节作物开花时间和种子休眠中的应用

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-10-24 DOI: 10.1007/s42994-020-00032-z

Olena Kishchenko, Yuzhen Zhou, Satyvaldy Jatayev, Yuri Shavrukov, Nikolai Borisjuk

引用次数: 8

On improving strigolactone mimics for induction of suicidal germination of the root parasitic plant Striga hermonthica 改进strigolactone模拟物诱导根寄生植物Striga hermonthica自杀性发芽的研究

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-10-17 DOI: 10.1007/s42994-020-00031-0

Ikuo Takahashi, Kosuke Fukui, Tadao Asami

{"title":"On improving strigolactone mimics for induction of suicidal germination of the root parasitic plant Striga hermonthica","authors":"Ikuo Takahashi, Kosuke Fukui, Tadao Asami","doi":"10.1007/s42994-020-00031-0","DOIUrl":"10.1007/s42994-020-00031-0","url":null,"abstract":"<div><p>Strigolactones (SLs) are plant hormones that regulate the branching of plants and seed germination stimulants of root parasitic plants. As root parasites are a great threat to agricultural production, the use of SL agonists could be anticipated to provide an efficient method for regulating root parasites as suicidal germination inducers. A series of phenoxyfuranone-type SL mimics, termed debranones, has been reported to show potent bioactivities, including reduction of the tiller number on rice, and stimulation of seed germination in the root parasite <i>Striga hermonthica.</i> To exert both activities, two substituents on the phenyl ring of the molecules were important but at least a substituent at the 2-position must be an electron-withdrawing group. However, little is known about the effect of the properties of the substituents at the 2-position on bioactivities. Here, we found that different substituents at the 2-position give different preferences for bioactivities. Halogenated debranones were more effective than the others and SL agonist GR24 for inhibiting rice tiller but far less effective in the induction of <i>S. hermonthica</i> germination. Meanwhile, nitrile and methyl derivatives clearly stimulated the germination of <i>S. hermonthica</i> seeds. Although their IC<sub>50</sub> values were 100 times higher than that of GR24 in the receptor competitive binding assay, their physiological activities were approximately 1/10 of GR24. These differences could be due to their uptake in plants and/or their physicochemical stability under our experimental conditions. These findings could support the design of more potent and selective SL agonists that could contribute to solving big agricultural issues.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"2 1","pages":"1 - 13"},"PeriodicalIF":3.6,"publicationDate":"2020-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00031-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50491008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Regulation of cell reprogramming by auxin during somatic embryogenesis 生长素在体细胞胚胎发生过程中对细胞重编程的调控

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-09-03 DOI: 10.1007/s42994-020-00029-8

Li Ping Tang, Xian Sheng Zhang, Ying Hua Su

{"title":"Regulation of cell reprogramming by auxin during somatic embryogenesis","authors":"Li Ping Tang, Xian Sheng Zhang, Ying Hua Su","doi":"10.1007/s42994-020-00029-8","DOIUrl":"10.1007/s42994-020-00029-8","url":null,"abstract":"<div><p>How somatic cells develop into a whole plant is a central question in plant developmental biology. This powerful ability of plant cells is recognized as their totipotency. Somatic embryogenesis is an excellent example and a good research system for studying plant cell totipotency. However, very little is known about the molecular basis of cell reprogramming from somatic cells to totipotent cells in this process. During somatic embryogenesis from immature zygotic embryos in <i>Arabidopsis</i>, exogenous auxin treatment is required for embryonic callus formation, but removal of exogenous auxin inducing endogenous auxin biosynthesis is essential for somatic embryo (SE) induction. Ectopic expression of specific transcription factor genes, such as “<i>LAFL</i>” and <i>BABY BOOM</i> (<i>BBM</i>), can induce SEs without exogenous growth regulators. Somatic embryogenesis can also be triggered by stress, as well as by disruption of chromatin remodeling, including PRC2-mediated histone methylation, histone deacetylation, and PKL-related chromatin remodeling. It is evident that embryonic identity genes are required and endogenous auxin plays a central role for cell reprogramming during the induction of SEs. Thus, we focus on reviewing the regulation of cell reprogramming for somatic embryogenesis by auxin.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 3","pages":"185 - 193"},"PeriodicalIF":3.6,"publicationDate":"2020-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00029-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9148585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Improving coordination of plant growth and nitrogen metabolism for sustainable agriculture 改善植物生长和氮代谢的协调，促进可持续农业

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-08-31 DOI: 10.1007/s42994-020-00027-w

Xiang Han, Kun Wu, Xiangdong Fu, Qian Liu

{"title":"Improving coordination of plant growth and nitrogen metabolism for sustainable agriculture","authors":"Xiang Han, Kun Wu, Xiangdong Fu, Qian Liu","doi":"10.1007/s42994-020-00027-w","DOIUrl":"10.1007/s42994-020-00027-w","url":null,"abstract":"<div><p>The agricultural green revolution of the 1960s boosted cereal crop yield was in part due to cultivation of semi-dwarf green revolution varieties. The semi-dwarf plants resist lodging and require high nitrogen (N) fertilizer inputs to maximize yield. To produce higher grain yield, inorganic fertilizer has been overused by Chinese farmers in intensive crop production. With the ongoing increase in the food demand of global population and the environmental pollution, improving crop productivity with reduced N supply is a pressing challenge. Despite a great deal of research efforts, to date only a few genes that improve N use efficiency (NUE) have been identified. The molecular mechanisms underlying the coordination of plant growth, carbon (C) and N assimilation is still not fully understood, thus preventing significant improvement. Recent advances have shed light on how explore NUE within an overall plant biology system that considered the co-regulation of plant growth, C and N metabolisms as a whole, rather than focusing specifically on N uptake and assimilation. There are several potential approaches to improve NUE discussed in this review. Increasing knowledge of how plants sense and respond to changes in N availability, as well as identifying new targets for breeding strategies to simultaneously improve NUE and grain yield, could usher in a new green revolution.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 4","pages":"255 - 275"},"PeriodicalIF":3.6,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00027-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9094531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The molecular regulation of cell pluripotency in plants 植物细胞多能性的分子调控

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-08-27 DOI: 10.1007/s42994-020-00028-9

Chongyi Xu, Yuxin Hu

引用次数: 9

Molecular and genetic pathways for optimizing spikelet development and grain yield 优化小穗发育和粮食产量的分子和遗传途径

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-07-31 DOI: 10.1007/s42994-020-00026-x

Zheng Yuan, Staffan Persson, Dabing Zhang

引用次数: 10

CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis 基于CRE/LOX的拟南芥根形成和再生过程中细胞谱系的分析

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-07-10 DOI: 10.1007/s42994-020-00025-y

Ning Zhai, Lin Xu

引用次数: 1