aBIOTECH最新文献_第7页

FERONIA cytoplasmic domain: node of varied signal outputs FERONIA细胞质结构域：不同信号输出的节点

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-03-25 DOI: 10.1007/s42994-020-00017-y

Jia Chen, Sirui Zhu, Zhenhua Ming, Xuanming Liu, Feng Yu

{"title":"FERONIA cytoplasmic domain: node of varied signal outputs","authors":"Jia Chen, Sirui Zhu, Zhenhua Ming, Xuanming Liu, Feng Yu","doi":"10.1007/s42994-020-00017-y","DOIUrl":"10.1007/s42994-020-00017-y","url":null,"abstract":"<div><p>The receptor-like kinase (RLK) FERONIA (FER), located on the plasma membrane, belongs to the <i>Catharanthus roseus</i> RLK1-like kinase family (<i>Cr</i>RLK1L) and participates in widespread biological processes in plants in a context-dependent fashion. Genetic studies in <i>Arabidopsis</i> illustrated the versatile roles that FER plays in fertilization, vegetative growth, defense and stress responses, cell-wall homeostasis, as well as protein synthesis. These studies also helped to identify genes and signal pathways involved in FER signal transduction. Despite increasingly larger numbers of studies discussing how FER senses its ligand, Rapid alkalinization factor (RALF) peptides, and further regulates downstream factors, few have shown the mechanisms of how FER mediates the specific regulation of downstream signals in context of the phosphorylation of its cytoplasmic domain. As understanding this would help in better understanding the diversity and complexity of FER function, this paper aims to review the roles of FER in regulating different signal outputs from the view of the role of its cytoplasmic domain.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 2","pages":"135 - 146"},"PeriodicalIF":3.6,"publicationDate":"2020-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-020-00017-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9093381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Efficient expression of multiple guide RNAs for CRISPR/Cas genome editing 用于CRISPR/Cas基因组编辑的多个引导RNA的有效表达

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2020-01-23 DOI: 10.1007/s42994-019-00014-w

Vicki Hsieh-Feng, Yinong Yang

{"title":"Efficient expression of multiple guide RNAs for CRISPR/Cas genome editing","authors":"Vicki Hsieh-Feng, Yinong Yang","doi":"10.1007/s42994-019-00014-w","DOIUrl":"10.1007/s42994-019-00014-w","url":null,"abstract":"<div><p>The Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein system (CRISPR/Cas) has recently become the most powerful tool available for genome engineering in various organisms. With efficient and proper expression of multiple guide RNAs (gRNAs), the CRISPR/Cas system is particularly suitable for multiplex genome editing. During the past several years, different CRISPR/Cas expression strategies, such as two-component transcriptional unit, single transcriptional unit, and bidirectional promoter systems, have been developed to efficiently express gRNAs as well as Cas nucleases. Significant progress has been made to optimize gRNA production using different types of promoters and RNA processing strategies such as ribozymes, endogenous RNases, and exogenous endoribonuclease (Csy4). Besides being constitutively and ubiquitously expressed, inducible and spatiotemporal regulations of gRNA expression have been demonstrated using inducible, tissue-specific, and/or synthetic promoters for specific research purposes. Most recently, the emergence of CRISPR/Cas ribonucleoprotein delivery methods, such as engineered nanoparticles, further revolutionized transgene-free and multiplex genome editing. In this review, we discuss current strategies and future perspectives for efficient expression and engineering of gRNAs with a goal to facilitate CRISPR/Cas-based multiplex genome editing.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 2","pages":"123 - 134"},"PeriodicalIF":3.6,"publicationDate":"2020-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00014-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9093385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Structures of plant resistosome reveal how NLR immune receptors are activated 植物抗性体的结构揭示了NLR免疫受体是如何被激活的

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-12-06 DOI: 10.1007/s42994-019-00012-y

Xuetao Shi, Suomeng Dong, Wende Liu

引用次数: 4

Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants 产生无转基因且基因稳定的CRISPR编辑植物的技术突破

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-12-03 DOI: 10.1007/s42994-019-00013-x

Yubing He, Yunde Zhao

{"title":"Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants","authors":"Yubing He, Yunde Zhao","doi":"10.1007/s42994-019-00013-x","DOIUrl":"10.1007/s42994-019-00013-x","url":null,"abstract":"<div><p>CRISPR/Cas9 gene-editing technologies have been very effective in editing target genes in all major crop plants and offer unprecedented potentials in crop improvement. A major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene free to maintain trait stability and to gain regulatory approval for commercial production. In this article, we present various strategies for generating transgene-free and target gene-edited crop plants. The <i>CRISPR</i> transgenes can be removed by genetic segregation if the crop plants are reproduced sexually. Marker-assisted tracking and eliminating transgenes greatly decrease the time and labor needed for identifying the ideal transgene-free plants. Transgenes can be programed to undergo self-elimination when <i>CRISPR</i> genes and suicide genes are sequentially activated, greatly accelerating the isolation of transgene-free and target gene-edited plants. Transgene-free plants can also be generated using approaches that are considered non-transgenic such as ribonucleoprotein transfection, transient expression of transgenes without DNA integration, and nano-biotechnology. Here, we discuss the advantages and disadvantages of the various strategies in generating transgene-free plants and provide guidance for adopting the best strategies in editing a crop plant.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"88 - 96"},"PeriodicalIF":3.6,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00013-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

CRISPR-Cas nucleases and base editors for plant genome editing 用于植物基因组编辑的CRISPR-Cas核酸酶和碱基编辑器

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-11-30 DOI: 10.1007/s42994-019-00010-0

Filiz Gürel, Yingxiao Zhang, Simon Sretenovic, Yiping Qi

{"title":"CRISPR-Cas nucleases and base editors for plant genome editing","authors":"Filiz Gürel, Yingxiao Zhang, Simon Sretenovic, Yiping Qi","doi":"10.1007/s42994-019-00010-0","DOIUrl":"10.1007/s42994-019-00010-0","url":null,"abstract":"<div><p>Clustered regularly interspaced short palindromic repeats (CRISPR)—CRISPR-associated protein (Cas) and base editors are fundamental tools in plant genome editing. Cas9 from <i>Streptococcus pyogenes</i> (SpCas9), recognizing an NGG protospacer adjacent motif (PAM), is a widely used nuclease for genome editing in living cells. Cas12a nucleases, targeting T-rich PAMs, have also been recently demonstrated in several plant species. Furthermore, multiple Cas9 and Cas12a engineered variants and orthologs, with different PAM recognition sites, editing efficiencies and fidelity, have been explored in plants. These RNA-guided sequence-specific nucleases (SSN) generate double-stranded breaks (DSBs) in DNA, which trigger non-homologous end-joining (NHEJ) repair or homology-directed repair (HDR), resulting in insertion and deletion (indel) mutations or precise gene replacement, respectively. Alternatively, genome editing can be achieved by base editors without introducing DSBs. So far, several base editors have been applied in plants to introduce C-to-T or A-to-G transitions, but they are still undergoing improvement in editing window size, targeting scope, off-target effects in DNA and RNA, product purity and overall activity. Here, we summarize recent progress on the application of Cas nucleases, engineered Cas variants and base editors in plants.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"74 - 87"},"PeriodicalIF":3.6,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00010-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9446914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives CRISPR/Cas基因组编辑技术在植物中的精确基因替换：现状和未来展望

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-11-07 DOI: 10.1007/s42994-019-00009-7

Shaoya Li, Lanqin Xia

{"title":"Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives","authors":"Shaoya Li, Lanqin Xia","doi":"10.1007/s42994-019-00009-7","DOIUrl":"10.1007/s42994-019-00009-7","url":null,"abstract":"<div><p>CRISPR/Cas, as a simple, versatile, robust and cost-effective system for genome manipulation, has dominated the genome editing field over the past few years. The application of CRISPR/Cas in crop improvement is particularly important in the context of global climate change, as well as diverse agricultural, environmental and ecological challenges. Various CRISPR/Cas toolboxes have been developed and allow for targeted mutagenesis at specific genome loci, transcriptome regulation and epigenome editing, base editing, and precise targeted gene/allele replacement or tagging in plants. In particular, precise replacement of an existing allele with an elite allele in a commercial variety through homology-directed repair (HDR) is a holy grail in genome editing for crop improvement as it has been very difficult, laborious and time-consuming to introgress the elite alleles into commercial varieties without any linkage drag from parental lines within a few generations in crop breeding practice. However, it still remains very challenging in crop plants. This review intends to provide an informative summary of the latest development and breakthroughs in gene replacement using CRISPR/Cas technology, with a focus on achievements, potential mechanisms and future perspectives in plant biological science as well as crop improvement.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"58 - 73"},"PeriodicalIF":3.6,"publicationDate":"2019-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00009-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Positional effects on efficiency of CRISPR/Cas9-based transcriptional activation in rice plants 水稻CRISPR/Cas9转录激活效率的定位效应

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-10-11 DOI: 10.1007/s42994-019-00007-9

Xiaoyu Gong, Tao Zhang, Jialing Xing, Rongchen Wang, Yunde Zhao

{"title":"Positional effects on efficiency of CRISPR/Cas9-based transcriptional activation in rice plants","authors":"Xiaoyu Gong, Tao Zhang, Jialing Xing, Rongchen Wang, Yunde Zhao","doi":"10.1007/s42994-019-00007-9","DOIUrl":"10.1007/s42994-019-00007-9","url":null,"abstract":"<div><p>The nuclease-dead Cas9 (dCas9) has been reprogrammed for transcriptional activation by fusing dCas9 to a transcriptional activation domain. In the presence of a guide RNA (gRNA), the dCas9 fusions specifically bind to regions of a promoter to activate transcription. Significant amount of effort has been directed toward the identification and optimization of the fusions of dCas9-activation domain, but very little is known about the impact of gRNA target positions within a promoter in plants on transcriptional activation efficiency. The dCas9–6TAL–VP128 system (dCas9-TV) has been optimized to activate transcription in plants. Here we use the dCas9-TV to activate transcription of <i>OsWOX11</i> and <i>OsYUC1</i>, two genes that cause dramatic developmental phenotypes when overexpressed. We designed a series of gRNAs targeting the promoters of the two genes. We show that gRNAs that target regions within 350 bp upstream of the transcription start site were most effective in transcriptional activation. Moreover, we show that using two gRNAs that simultaneously target two discrete sites in a promoter can further enhance transcription. This work provides guidelines for designed transcriptional activation through CRISPR/dCas9 systems.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"1 - 5"},"PeriodicalIF":3.6,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00007-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9088390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Genome editing in grass plants 草植物基因组编辑

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-09-24 DOI: 10.1007/s42994-019-00005-x

Si Nian Char, Bing Yang

{"title":"Genome editing in grass plants","authors":"Si Nian Char, Bing Yang","doi":"10.1007/s42994-019-00005-x","DOIUrl":"10.1007/s42994-019-00005-x","url":null,"abstract":"<div><p>Cereal crops including maize, rice, wheat, sorghum, barley, millet, oats and rye are the major calorie sources in our daily life and also important bioenergy sources of the world. The rapidly advancing and state-of-the-art genome-editing tools such as zinc finger nucleases, TAL effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (CRISPR-Cas9-, CRISPR-Cas12a- and CRISPR/Cas-derived base editors) have accelerated the functional genomics and have promising potential for precision breeding of grass crops. With the availability of annotated genomes of the major cereal crops, application of these established genome-editing toolkits to grass plants holds promise to increase the nutritional value and productivity. Furthermore, these easy-to-use and robust genome-editing toolkits have advanced the reverse genetics for discovery of novel gene functions in crop plants. In this review, we document some of important progress in development and utilization of genome-editing tool sets in grass plants. We also highlight present and future uses of genome-editing toolkits that can sustain and improve the quality of cereal grain for food consumption.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"41 - 57"},"PeriodicalIF":3.6,"publicationDate":"2019-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00005-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

General and specialized tyrosine metabolism pathways in plants 植物中一般和特殊的酪氨酸代谢途径

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-09-06 DOI: 10.1007/s42994-019-00006-w

Jing-Jing Xu, Xin Fang, Chen-Yi Li, Lei Yang, Xiao-Ya Chen

引用次数: 41

The working dead: repurposing inactive CRISPR-associated nucleases as programmable transcriptional regulators in plants 正在工作的死者：将非活性CRISPR相关核酸酶重新用作植物中的可编程转录调节因子

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2019-08-09 DOI: 10.1007/s42994-019-00003-z

Zhenxiang Li, Xiangyu Xiong, Jian-Feng Li

{"title":"The working dead: repurposing inactive CRISPR-associated nucleases as programmable transcriptional regulators in plants","authors":"Zhenxiang Li, Xiangyu Xiong, Jian-Feng Li","doi":"10.1007/s42994-019-00003-z","DOIUrl":"10.1007/s42994-019-00003-z","url":null,"abstract":"<div><p>Targeted gene manipulation is highly desirable for fundamental plant research, plant synthetic biology, and molecular breeding. The clustered regularly interspaced short palindromic repeats-associated (Cas) nuclease is a revolutionary tool for genome editing, and has received snowballing popularity for gene knockout applications in diverse organisms including plants. Recently, the nuclease-dead Cas (dCas) proteins have been repurposed as programmable transcriptional regulators through translational fusion with portable transcriptional repression or activation domains, which has paved new ways for flexible and multiplex control over the activities of target genes of interest without the need to generate DNA lesions. Here, we review the most important breakthroughs of dCas transcriptional regulators in non-plant organisms and recent accomplishments of this growing field in plants. We also provide perspectives on future development directions of dCas transcriptional regulators in plant research in hope to stimulate their quick evolution and broad applications.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"1 1","pages":"32 - 40"},"PeriodicalIF":3.6,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s42994-019-00003-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7