Yilin Shen, Tao Ye, Zihan Li, Torotwa Herman Kimutai, Hao Song, Xiaoou Dong, Jianmin Wan
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引用次数: 0
Abstract
Genome editing holds great promise for the molecular breeding of plants, yet its application is hindered by the shortage of simple and effective means of delivering genome editing reagents into plants. Conventional plant transformation-based methods for delivery of genome editing reagents into plants often involve prolonged tissue culture, a labor-intensive and technically challenging process for many elite crop cultivars. In this review, we describe various virus-based methods that have been employed to deliver genome editing reagents, including components of the CRISPR/Cas machinery and donor DNA for precision editing in plants. We update the progress in these methods with recent successful examples of genome editing achieved through virus-based delivery in different plant species, highlight the advantages and limitations of these delivery approaches, and discuss the remaining challenges.
基因组编辑为植物的分子育种带来了巨大希望,但由于缺乏简单有效的方法将基因组编辑试剂输送到植物体内,基因组编辑的应用受到了阻碍。将基因组编辑试剂输送到植物体内的传统植物转化方法往往涉及长时间的组织培养,对于许多优良作物栽培品种来说,这是一个劳动密集型且具有技术挑战性的过程。在本综述中,我们介绍了各种基于病毒的基因组编辑试剂输送方法,包括用于植物精准编辑的 CRISPR/Cas 机器和供体 DNA 的组件。我们介绍了这些方法的最新进展,以及最近在不同植物物种中通过病毒递送实现基因组编辑的成功实例,强调了这些递送方法的优势和局限性,并讨论了仍然存在的挑战。