玉米转录因子 ZmbHLH36 的异源表达可增强拟南芥的非生物胁迫耐受性

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhenggang Dai, Keyong Zhao, Dengyu Zheng, Siyu Guo, Huawen Zou, Zhongyi Wu, Chun Zhang
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引用次数: 0

摘要

碱性螺旋环螺旋(bHLH)转录因子广泛分布于真核生物中,在植物中,它们调控着许多生物过程,如细胞分化、发育、新陈代谢和应激反应。很少有研究关注 bHLH 转录因子在调控玉米(Zea mays)生长、发育和应激反应中的作用,尽管这些信息对玉米育种计划大有裨益。在这项研究中,我们克隆了玉米转录因子基因 ZmbHLH36(基因编号:100193615,GRMZM2G008691)。ZmbHLH36 具有 bHLH 家族特有的保守结构域。RT-qPCR 分析显示,ZmbHLH36 在玉米根部的表达水平最高,并且在各种非生物胁迫条件下表现出不同的表达模式。异源表达 ZmbHLH36 的转基因拟南芥(Arabidopsis thaliana)植株在 0.1 和 0.15 mol L-1 NaCl 处理以及 0.2 mol L-1 甘露醇处理下的根明显长于相应的非转基因植株。对胁迫下土壤生长植株的表型分析表明,与相应的非转基因植株相比,携带 ZmbHLH36 的转基因拟南芥植株的耐旱性和耐盐性明显增强。与相应的非转基因植株相比,表达 ZmbHLH36 的拟南芥植株丙二醛含量更低,过氧化物酶活性更高。ZmbHLH36 在玉米原生质体中表达时会定位于细胞核。本研究系统分析了ZmbHLH36对转基因拟南芥根系生长、发育和胁迫反应的影响,为进一步分析其在玉米中的作用和分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis

Basic helix-loop-helix (bHLH) transcription factors are widely distributed in eukaryotes, and in plants, they regulate many biological processes, such as cell differentiation, development, metabolism, and stress responses. Few studies have focused on the roles of bHLH transcription factors in regulating growth, development, and stress responses in maize (Zea mays), even though such information would greatly benefit maize breeding programs. In this study, we cloned the maize transcription factor gene ZmbHLH36 (Gene ID: 100193615, GRMZM2G008691). ZmbHLH36 possesses conserved domains characteristic of the bHLH family. RT-qPCR analysis revealed that ZmbHLH36 was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress conditions. Transgenic Arabidopsis (Arabidopsis thaliana) plants heterologously expressing ZmbHLH36 had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L−1 NaCl treatment as well as 0.2 mol L−1 mannitol treatment. Phenotypic analysis of soil-grown plants under stress showed that transgenic Arabidopsis plants harboring ZmbHLH36 exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic plants. Malondialdehyde contents were lower and peroxidase activity was higher in ZmbHLH36-expressing Arabidopsis plants than in the corresponding non-transgenic plants. ZmbHLH36 localized to the nucleus when expressed in maize protoplasts. This study provides a systematic analysis of the effects of ZmbHLH36 on root growth, development, and stress responses in transgenic Arabidopsis, laying a foundation for further analysis of its roles and molecular mechanisms in maize.

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CiteScore
7.70
自引率
2.80%
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