aBIOTECH最新文献_第6页

Predicting rice diseases using advanced technologies at different scales: present status and future perspectives 利用不同规模的先进技术预测水稻病害：现状与未来展望。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-11-29 DOI: 10.1007/s42994-023-00126-4

Ruyue Li, Sishi Chen, Haruna Matsumoto, Mostafa Gouda, Yusufjon Gafforov, Mengcen Wang, Yufei Liu

引用次数: 0

The RNA-binding domain of DCL3 is required for long-distance RNAi signaling DCL3的RNA结合域是长距离RNAi信号传导所必需的。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-11-28 DOI: 10.1007/s42994-023-00124-6

Jie Li, Bo-Sen Zhang, Hua-Wei Wu, Cheng-Lan Liu, Hui-Shan Guo, Jian-Hua Zhao

{"title":"The RNA-binding domain of DCL3 is required for long-distance RNAi signaling","authors":"Jie Li, Bo-Sen Zhang, Hua-Wei Wu, Cheng-Lan Liu, Hui-Shan Guo, Jian-Hua Zhao","doi":"10.1007/s42994-023-00124-6","DOIUrl":"10.1007/s42994-023-00124-6","url":null,"abstract":"<div><p>Small RNA (sRNA)-mediated RNA silencing (also known as RNA interference, or RNAi) is a conserved mechanism in eukaryotes that includes RNA degradation, DNA methylation, heterochromatin formation and protein translation repression. In plants, sRNAs can move either cell-to-cell or systemically, thereby acting as mobile silencing signals to trigger noncell autonomous silencing. However, whether and what proteins are also involved in noncell autonomous silencing have not been elucidated. In this study, we utilized a previously reported inducible RNAi plant, <i>PDSi</i>, which can induce systemic silencing of the endogenous <i>PDS</i> gene, and we demonstrated that DCL3 is involved in systemic <i>PDS</i> silencing through its RNA binding activity. We confirmed that the C-terminus of DCL3, including the predicted RNA-binding domain, is capable of binding short RNAs. Mutations affecting RNA binding, but not processing activity, reduced systemic <i>PDS</i> silencing, indicating that DCL3 binding to RNAs is required for the induction of systemic silencing. Cucumber mosaic virus infection assays showed that the RNA-binding activity of DCL3 is required for antiviral RNAi in systemically noninoculated leaves. Our findings demonstrate that DCL3 acts as a signaling agent involved in noncell autonomous silencing and an antiviral effect in addition to its previously known function in the generation of 24-nucleotide sRNAs.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10987413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of seed traits in soybean 大豆种子性状的调控

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-11-27 DOI: 10.1007/s42994-023-00122-8

Yang Hu, Yue Liu, Jun-Jie Wei, Wan-Ke Zhang, Shou-Yi Chen, Jin-Song Zhang

引用次数: 0

Regulation of regeneration in Arabidopsis thaliana 拟南芥的再生调节。

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-11-22 DOI: 10.1007/s42994-023-00121-9

Md Khairul Islam, Sai Teja Mummadi, Sanzhen Liu, Hairong Wei

{"title":"Regulation of regeneration in Arabidopsis thaliana","authors":"Md Khairul Islam, Sai Teja Mummadi, Sanzhen Liu, Hairong Wei","doi":"10.1007/s42994-023-00121-9","DOIUrl":"10.1007/s42994-023-00121-9","url":null,"abstract":"<div><p>We employed several algorithms with high efficacy to analyze the public transcriptomic data, aiming to identify key transcription factors (TFs) that regulate regeneration in <i>Arabidopsis thaliana</i>. Initially, we utilized CollaborativeNet, also known as TF-Cluster, to construct a collaborative network of all TFs, which was subsequently decomposed into many subnetworks using the Triple-Link and Compound Spring Embedder (CoSE) algorithms. Functional analysis of these subnetworks led to the identification of nine subnetworks closely associated with regeneration. We further applied principal component analysis and gene ontology (GO) enrichment analysis to reduce the subnetworks from nine to three, namely subnetworks 1, 12, and 17. Searching for TF-binding sites in the promoters of the co-expressed and co-regulated (CCGs) genes of all TFs in these three subnetworks and Triple-Gene Mutual Interaction analysis of TFs in these three subnetworks with the CCGs involved in regeneration enabled us to rank the TFs in each subnetwork. Finally, six potential candidate TFs—WOX9A, LEC2, PGA37, WIP5, PEI1, and AIL1 from subnetwork 1—were identified, and their roles in somatic embryogenesis (GO:0010262) and regeneration (GO:0031099) were discussed, so were the TFs in Subnetwork 12 and 17 associated with regeneration. The TFs identified were also assessed using the CIS-BP database and Expression Atlas. Our analyses suggest some novel TFs that may have regulatory roles in regeneration and embryogenesis and provide valuable data and insights into the regulatory mechanisms related to regeneration. The tools and the procedures used here are instrumental for analyzing high-throughput transcriptomic data and advancing our understanding of the regulation of various biological processes of interest.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staying hungry: a roadmap to harnessing central regulators of symbiotic nitrogen fixation under fluctuating nitrogen availability 保持饥饿：在氮供应波动条件下利用共生固氮中心调节器的路线图

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-11-18 DOI: 10.1007/s42994-023-00123-7

Lijin Qiao, Jieshun Lin, Takuya Suzaki, Pengbo Liang

{"title":"Staying hungry: a roadmap to harnessing central regulators of symbiotic nitrogen fixation under fluctuating nitrogen availability","authors":"Lijin Qiao, Jieshun Lin, Takuya Suzaki, Pengbo Liang","doi":"10.1007/s42994-023-00123-7","DOIUrl":"10.1007/s42994-023-00123-7","url":null,"abstract":"<div><p>Legumes have evolved specific inventions to enhance nitrogen (N) acquisition by establishing symbiotic interactions with N-fixing rhizobial bacteria. Because symbiotic N fixation is energetically costly, legumes have developed sophisticated mechanisms to ensure carbon–nitrogen balance, in a variable environment, both locally and at the whole plant level, by monitoring nodule number, nodule development, and nodular nitrogenase activity, as well as controlling nodule senescence. Studies of the autoregulation of nodulation and regulation of nodulation by nodule inception (NIN) and NIN-LIKE PROTEINs (NLPs) have provided great insights into the genetic mechanisms underlying the nitrate-induced regulation of root nodulation for adapting to N availability in the rhizosphere. However, many aspects of N-induced pleiotropic regulation remain to be fully explained, such as N-triggered senescence in mature nodules. Wang et al. determined that this process is governed by a transcriptional network regulated by NAC-type transcription factors. Characterization and dissection of these soybean nitrogen-associated NAPs (SNAPs) transcription factor-mastered networks have yielded a roadmap for exploring how legumes rewire nodule functions across a range of N levels, laying the foundation for enhancing the growth of N-deprived crops in agricultural settings.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00123-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139261479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tomato leaf curl New Delhi virus: an emerging plant begomovirus threatening cucurbit production 番茄卷叶新德里病毒:一种威胁瓜类生产的新兴植物begomavirus。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-10-25 DOI: 10.1007/s42994-023-00118-4

Lingmin Cai, Yuzhen Mei, Ruyi Ye, Yun Deng, Xuejun Zhang, Zhongyuan Hu, Xueping Zhou, Mingfang Zhang, Jinghua Yang

{"title":"Tomato leaf curl New Delhi virus: an emerging plant begomovirus threatening cucurbit production","authors":"Lingmin Cai, Yuzhen Mei, Ruyi Ye, Yun Deng, Xuejun Zhang, Zhongyuan Hu, Xueping Zhou, Mingfang Zhang, Jinghua Yang","doi":"10.1007/s42994-023-00118-4","DOIUrl":"10.1007/s42994-023-00118-4","url":null,"abstract":"<div><p>Tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus, was first reported to infect tomato and has recently spread rapidly as an emerging disease to <i>Cucurbitaceae</i> crops. To date, the virus has been reported to infect more than 11 cucurbit crops, in 16 countries and regions, causing severe yield losses. In autumn 2022, ToLCNDV was first isolated from cucurbit plants in Southeastern coastal areas of China. Phylogenetic analysis established that these isolates belong to the Asian ToLCNDV clade, and shared high nucleotide identity and closest genetic relationship with the DNA-A sequence from the Chinese tomato-infecting ToLCNDV isolate (Accession no. OP356207) and the tomato New Delhi ToLCNDV-Severe isolate (Accession no. HM159454). In this review, we summarize the occurrence and distribution, host range, detection and diagnosis, control strategies, and genetic resistance of ToLCNDV in the <i>Cucurbitaceae</i>. We then summarize pathways that could be undertaken to improve our understanding of this emerging disease, with the objective to develop ToLCNDV-resistant cucurbit cultivars<i>.</i></p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-expression of GR79 EPSPS and GAT generates high glyphosate-resistant alfalfa with low glyphosate residues GR79 EPSPS 和 GAT 的共表达可产生低草甘膦残留的高草甘膦抗性紫花苜蓿

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-10-19 DOI: 10.1007/s42994-023-00119-3

Yingying Meng, Wenwen Zhang, Zhaoming Wang, Feng Yuan, Sandui Guo, Hao Lin, Lifang Niu

引用次数: 0

Application of Wox2a in transformation of recalcitrant maize genotypes 将 Wox2a 应用于转化难以克服的玉米基因型

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-10-13 DOI: 10.1007/s42994-023-00116-6

Qiangbo Liu, Xian Sheng Zhang, Ying Hua Su

引用次数: 0

Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification MdMYB73 对液泡质子泵 P-ATP 酶 MdPH5 的调控及其在苹果酸积累和液泡酸化中的作用

IF 4.6 4区农林科学

aBIOTECH Pub Date : 2023-09-22 DOI: 10.1007/s42994-023-00115-7

Xiao-Yu Huang, Ying Xiang, Yu-Wen Zhao, Chu-Kun Wang, Jia-Hui Wang, Wen-Yan Wang, Xiao-Long Liu, Quan Sun, Da-Gang Hu

{"title":"Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification","authors":"Xiao-Yu Huang, Ying Xiang, Yu-Wen Zhao, Chu-Kun Wang, Jia-Hui Wang, Wen-Yan Wang, Xiao-Long Liu, Quan Sun, Da-Gang Hu","doi":"10.1007/s42994-023-00115-7","DOIUrl":"10.1007/s42994-023-00115-7","url":null,"abstract":"<div><p>As the main organic acid in fruits, malate is produced in the cytoplasm and is then transported into the vacuole. It accumulates by vacuolar proton pumps, transporters, and channels, affecting the taste and flavor of fruits. Among the three types of proton pumps (V-ATPases, V-PPases, and P-ATPases), the P-ATPases play an important role in the transport of malate into vacuoles. In this study, the transcriptome data, collected at different stages after blooming and during storage, were analyzed and the results demonstrated that the expression of <i>MdPH5</i>, a vacuolar proton-pumping P-ATPase, was associated with both pre- and post-harvest malate contents. Moreover, MdPH5 is localized at the tonoplast and regulates malate accumulation and vacuolar pH. In addition, MdMYB73, an upstream MYB transcription factor of <i>MdPH5</i>, directly binds to its promoter, thereby transcriptionally activating its expression and enhancing its activity. In this way, MdMYB73 can also affect malate accumulation and vacuolar pH. Overall, this study clarifies how MdMYB73 and MdPH5 act to regulate vacuolar malate transport systems, thereby affecting malate accumulation and vacuolar pH.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-023-00115-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136059473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plants 植物黄萎病毒力的DNA甲基化表观遗传调控。

IF 3.6 4区农林科学

aBIOTECH Pub Date : 2023-09-20 DOI: 10.1007/s42994-023-00117-5

Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duan

{"title":"DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plants","authors":"Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duan","doi":"10.1007/s42994-023-00117-5","DOIUrl":"10.1007/s42994-023-00117-5","url":null,"abstract":"<div><p>As a conserved epigenetic mark, DNA cytosine methylation, at the 5’ position (5-mC), plays important roles in multiple biological processes, including plant immunity. However, the involvement of DNA methylation in the determinants of virulence of phytopathogenic fungi remains elusive. In this study, we profiled the DNA methylation patterns of the phytopathogenic fungus <i>Verticillium dahliae</i>, one of the major causal pathogens of <i>Verticillium</i> wilt disease that causes great losses in many crops, and explored its contribution in fungal pathogenicity. We reveal that DNA methylation modification is present in <i>V. dahliae</i> and is required for its full virulence in host plants. The major enzymes responsible for the establishment of DNA methylation in <i>V. dahliae</i> were identified. We provided evidence that DNA methyltransferase-mediated establishment of DNA methylation pattern positively regulates fungal virulence, mainly through repressing a conserved protein kinase VdRim15-mediated Ca<sup>2+</sup> signaling and ROS production, which is essential for the penetration activity of <i>V. dahliae</i>. In addition, we further demonstrated that histone H3 lysine 9 trimethylation (H3K9me3), another heterochromatin marker that is closely associated with 5-mC in eukaryotes, also participates in the regulation of <i>V. dahliae</i> pathogenicity, through a similar mechanism. More importantly, DNA methyltransferase genes <i>VdRid</i>, <i>VdDnmt5,</i> as well as H3K9me3 methyltransferase genes, were greatly induced during the early infection phase, implying that a dynamic regulation of 5-mC and H3K9me3 homeostasis is required for an efficient infection. Collectively, our findings uncover an epigenetic mechanism in the regulation of phytopathogenic fungal virulence.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10638132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134650523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0