Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution in Arabidopsis

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yukang Wang, Ronghui Pan, Jianping Hu
{"title":"Impact of acute heat stress on mitochondrial function, ultrastructure and cardiolipin distribution in Arabidopsis","authors":"Yukang Wang,&nbsp;Ronghui Pan,&nbsp;Jianping Hu","doi":"10.1007/s42994-024-00151-x","DOIUrl":null,"url":null,"abstract":"<div><p>Besides providing energy to sustain life, mitochondria also play crucial roles in stress response and programmed cell death. The mitochondrial hallmark lipid, cardiolipin (CL), is essential to the maintenance of mitochondrial structure and function. However, how mitochondria and CL are involved in stress response is not as well defined in plants as in animal and yeast cells. We previously revealed a role for CL in mitochondrial fission and in heat stress response in <i>Arabidopsis</i>. To further determine the involvement of mitochondria and CL in plant heat response, here we treated <i>Arabidopsis</i> seedlings with varied lengths of acute heat stress. These treatments resulted in decreases in mitochondrial membrane potential, disruption of mitochondrial ultrastructure, accumulation of mitochondrial reactive-oxygen species (ROS), and redistribution of CL to the outer mitochondrial membrane and to a novel type of vesicle. The level of the observed changes correlated with the severeness of the heat stress, indicating the strong relevance of these processes to stress response. Our findings provide the basis for studying mechanisms underpinning the role of mitochondria and CL in plant stress response.</p></div>","PeriodicalId":53135,"journal":{"name":"aBIOTECH","volume":"5 3","pages":"362 - 367"},"PeriodicalIF":4.6000,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s42994-024-00151-x.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"aBIOTECH","FirstCategoryId":"1091","ListUrlMain":"https://link.springer.com/article/10.1007/s42994-024-00151-x","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Besides providing energy to sustain life, mitochondria also play crucial roles in stress response and programmed cell death. The mitochondrial hallmark lipid, cardiolipin (CL), is essential to the maintenance of mitochondrial structure and function. However, how mitochondria and CL are involved in stress response is not as well defined in plants as in animal and yeast cells. We previously revealed a role for CL in mitochondrial fission and in heat stress response in Arabidopsis. To further determine the involvement of mitochondria and CL in plant heat response, here we treated Arabidopsis seedlings with varied lengths of acute heat stress. These treatments resulted in decreases in mitochondrial membrane potential, disruption of mitochondrial ultrastructure, accumulation of mitochondrial reactive-oxygen species (ROS), and redistribution of CL to the outer mitochondrial membrane and to a novel type of vesicle. The level of the observed changes correlated with the severeness of the heat stress, indicating the strong relevance of these processes to stress response. Our findings provide the basis for studying mechanisms underpinning the role of mitochondria and CL in plant stress response.

急性热胁迫对拟南芥线粒体功能、超微结构和心磷脂分布的影响
线粒体除了为维持生命提供能量外,还在应激反应和细胞程序性死亡中发挥着至关重要的作用。线粒体的标志性脂质--心磷脂(CL)对线粒体结构和功能的维持至关重要。然而,线粒体和 CL 如何参与应激反应在植物中还没有像在动物和酵母细胞中那样明确。我们之前揭示了 CL 在拟南芥线粒体裂变和热胁迫响应中的作用。为了进一步确定线粒体和 CL 在植物热响应中的作用,我们对拟南芥幼苗进行了不同时间长度的急性热胁迫处理。这些处理导致线粒体膜电位下降、线粒体超微结构破坏、线粒体活性氧(ROS)积累、CL重新分布到线粒体外膜和一种新型囊泡。观察到的变化程度与热应激的严重程度相关,表明这些过程与应激反应密切相关。我们的发现为研究线粒体和 CL 在植物胁迫响应中的作用机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.70
自引率
2.80%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信