The maize ZmCPK39-ZmKnox2 module regulates plant height

IF 4.6 4区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

aBIOTECH Pub Date : 2024-03-15 DOI:10.1007/s42994-024-00150-y

Mang Zhu, Chenyu Guo, Xiaohui Zhang, Yulin Liu, Xiaohui Jiang, Limei Chen, Mingliang Xu

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Abstract

Plant height is an important agronomic trait that affects high-density tolerance and lodging resistance. However, the regulators and their underlying molecular mechanisms controlling plant height in maize remain understudied. Here, we report that knockout mutants of the calcium-dependent protein kinase gene ZmCPK39 (ZmCPK39-KO) exhibit dramatically reduced plant height, characterized by shorter internodes and a slight decrease in node numbers. Furthermore, we identified a ZmCPK39-interacting protein, the knotted-related homeobox (ZmKnox2), and observed that plant height was also significantly reduced in a mutator transposon-inserted mutant of ZmKnox2 (ZmKnox2-Mu). Combined analysis of transcriptomic and metabonomic data indicates that multiple phytohormone signaling and photosynthesis pathways are disrupted in both ZmCPK39-KO and ZmKnox2-Mu mutants. Taken together, these results provide new insights into the function of ZmCPK39 and identify potential targets for breeding lodging-resistant and high-density tolerant maize cultivars.

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玉米 ZmCPK39-ZmKnox2 模块调控植株高度

株高是影响高密度耐受性和抗倒伏性的重要农艺性状。然而，控制玉米株高的调节因子及其潜在的分子机制仍未得到充分研究。在此，我们报告了钙依赖性蛋白激酶基因 ZmCPK39（ZmCPK39-KO）的基因敲除突变体表现出显著的植株高度降低，其特征是节间变短和节数略有减少。此外，我们还发现了一种与 ZmCPK39 相互作用的蛋白--结相关同源染色体（ZmKnox2），并观察到 ZmKnox2 的突变体转座子插入突变体（ZmKnox2-Mu）的株高也显著降低。对转录组和代谢组数据的综合分析表明，ZmCPK39-KO和ZmKnox2-Mu突变体的多种植物激素信号转导和光合作用途径都受到了干扰。综上所述，这些结果为了解 ZmCPK39 的功能提供了新的视角，并为培育抗倒伏和高密度耐受性玉米栽培品种确定了潜在的目标。

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来源期刊

aBIOTECH

CiteScore

7.70

自引率

2.80%

发文量