玉米 ZmCPK39-ZmKnox2 模块调控植株高度

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mang Zhu, Chenyu Guo, Xiaohui Zhang, Yulin Liu, Xiaohui Jiang, Limei Chen, Mingliang Xu
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引用次数: 0

摘要

株高是影响高密度耐受性和抗倒伏性的重要农艺性状。然而,控制玉米株高的调节因子及其潜在的分子机制仍未得到充分研究。在此,我们报告了钙依赖性蛋白激酶基因 ZmCPK39(ZmCPK39-KO)的基因敲除突变体表现出显著的植株高度降低,其特征是节间变短和节数略有减少。此外,我们还发现了一种与 ZmCPK39 相互作用的蛋白--结相关同源染色体(ZmKnox2),并观察到 ZmKnox2 的突变体转座子插入突变体(ZmKnox2-Mu)的株高也显著降低。对转录组和代谢组数据的综合分析表明,ZmCPK39-KO和ZmKnox2-Mu突变体的多种植物激素信号转导和光合作用途径都受到了干扰。综上所述,这些结果为了解 ZmCPK39 的功能提供了新的视角,并为培育抗倒伏和高密度耐受性玉米栽培品种确定了潜在的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The maize ZmCPK39-ZmKnox2 module regulates plant height

Plant height is an important agronomic trait that affects high-density tolerance and lodging resistance. However, the regulators and their underlying molecular mechanisms controlling plant height in maize remain understudied. Here, we report that knockout mutants of the calcium-dependent protein kinase gene ZmCPK39 (ZmCPK39-KO) exhibit dramatically reduced plant height, characterized by shorter internodes and a slight decrease in node numbers. Furthermore, we identified a ZmCPK39-interacting protein, the knotted-related homeobox (ZmKnox2), and observed that plant height was also significantly reduced in a mutator transposon-inserted mutant of ZmKnox2 (ZmKnox2-Mu). Combined analysis of transcriptomic and metabonomic data indicates that multiple phytohormone signaling and photosynthesis pathways are disrupted in both ZmCPK39-KO and ZmKnox2-Mu mutants. Taken together, these results provide new insights into the function of ZmCPK39 and identify potential targets for breeding lodging-resistant and high-density tolerant maize cultivars.

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来源期刊
CiteScore
7.70
自引率
2.80%
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