Virus Genes最新文献_第2页

Roles of the M2 protein of influenza A virus. 甲型流感病毒M2蛋白的作用。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-05-20 DOI: 10.1007/s11262-026-02243-w

Weigang Ren, Qin Wang, Yongxiang Yi, Junwei Li

{"title":"Roles of the M2 protein of influenza A virus.","authors":"Weigang Ren, Qin Wang, Yongxiang Yi, Junwei Li","doi":"10.1007/s11262-026-02243-w","DOIUrl":"10.1007/s11262-026-02243-w","url":null,"abstract":"The matrix protein 2 (M2) is one of the most representative and widely studied ion channel proteins, which plays broad biological roles in the life cycle of the influenza A virus. The M2 protein affects the function of host cells and the regulation of cellular homeostasis in an ion channel activity-dependent or-independent manner and it is also the target for anti-influenza virus drugs, such as amantadine and rimantadine. The focus of this review is to summarize the research progress on the M2 protein, such as structure and function, vaccine and drug development as well as antagonist of host innate immune responses. A particular emphasis is placed on the multifaceted roles of M2 beyond canonical ion conduction. Specifically, M2 modulates the MAVS pathway through direct interaction with MAVS and by inducing ROS production, which amplifies interferon-mediated inflammation while paradoxically disrupting autophagic degradation of MAVS aggregation. Concurrently, the M2 protein orchestrates autophagy regulation by inhibiting autophagosome-lysosome fusion and initiating AKT-mTOR signaling, thus stabilizing the viral polymerase to enhance viral replication. A comprehensive understanding of these mechanisms is essential for advancing universal M2-based vaccine strategies and novel host-directed antiviral therapies.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"561-575"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147984318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine epidemic diarrhea virus influences the expression of tight junction proteins through the polarity protein PAR6. 猪流行性腹泻病毒通过极性蛋白PAR6影响紧密连接蛋白的表达。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-09 DOI: 10.1007/s11262-026-02254-7

Tingyu Hu, Ruolan Zhang, Qian Weng, Simin Wang, Yuheng Li, Zhihua Pei, Kai Wang, Guixue Hu

{"title":"Porcine epidemic diarrhea virus influences the expression of tight junction proteins through the polarity protein PAR6.","authors":"Tingyu Hu, Ruolan Zhang, Qian Weng, Simin Wang, Yuheng Li, Zhihua Pei, Kai Wang, Guixue Hu","doi":"10.1007/s11262-026-02254-7","DOIUrl":"10.1007/s11262-026-02254-7","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) is recognized as the primary pathogen causing porcine diarrhea worldwide. For the health of the intestinal mucosa, tight junction proteins are essential. Nevertheless, little is known about the precise processes by which PEDV influences tight junction proteins in epithelial cells. This study aims to investigate the effects of porcine epidemic diarrhea virus (PEDV) infection on the expression of the polarity protein Par6 in IPEC-J2 cells, and to determine whether it influences the expression of tight junction and actin proteins by regulating PAR6. RT-qPCR was used to detect the expression levels of PAR6 and PATJ mRNA. Western blotting and immunofluorescence techniques were used to analyze PAR6 protein expression and localization. Results showed that compared to the control group, PAR6 expression was upregulated and accompanied by abnormal intracellular localization. Subsequently, siRNA-PAR6 and PAR6 overexpression plasmids were constructed. Their effects on tight junction proteins (ZO-1, ZO-2, ZO-3, Occludin, Claudin-1, Claudin-3) and F-actin were evaluated via qPCR and Western blotting. The results demonstrated that, following PEDV infection, PAR6 overexpression and PAR6 knockdown exerted opposite effects on the regulation of the three genes ZO-3, Claudin-1, and Occludin. In summary, PEDV modulates the expression of tight junction proteins (ZO-3, Claudin-1, and Occludin) by regulating the polarity molecule PAR6. This finding deepens our understanding of PEDV pathogenesis and facilitates the development of more effective therapeutics.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"639-648"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148413052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequence analysis of baculoviruses from the noctuid moths Eudryas unio and Autographa gamma indicates that they are isolates of Betabaculovirus xecnigri, a species of slow-killing granuloviruses. 夜蛾Eudryas unio和Autographa gamma杆状病毒的基因组序列分析表明，它们是一种慢杀颗粒病毒Betabaculovirus xecnigri的分离株。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-22 DOI: 10.1007/s11262-026-02260-9

Robert L Harrison, Charlotte B Francoeur, Daniel L Rowley

{"title":"Genome sequence analysis of baculoviruses from the noctuid moths Eudryas unio and Autographa gamma indicates that they are isolates of Betabaculovirus xecnigri, a species of slow-killing granuloviruses.","authors":"Robert L Harrison, Charlotte B Francoeur, Daniel L Rowley","doi":"10.1007/s11262-026-02260-9","DOIUrl":"10.1007/s11262-026-02260-9","url":null,"abstract":"Complete genome sequences were determined for three uncharacterized betabaculoviruses (Baculoviridae: Lefavirales) from noctuid moth species. These viruses included Eudryas unio granulovirus 1112 (EuunGV-1112), Eudryas unio granulovirus 1229 (EuunGV-1229), and Autographa gamma granulovirus Darmstadt (AugaGV-Darmstadt). Analysis of the features and open reading frame (ORF) contents of the genome sequences indicated that all three viruses were closely related to Helicoverpa armigera granulovirus (HearGV; Betabaculovirus helarmigerae) and Xestia c-nigrum granulovirus (XecnGV; Betabaculovirus xecnigri), two betabaculoviruses that kill larvae slowly and appear to suppress alphabaculovirus replication in coinfections. Phylogenetic inference with whole genome or core gene alignments grouped the EuunGV isolates in a clade with XecnGV. This clade was contained within a larger clade with AugaGV. Pairwise nucleotide distance estimation confirmed that AugaGV and the EuunGV isolates are viruses of Betabaculovirus xecnigri. Five ORFs were found to be unique to the EuunGV/AugaGV/HearGV/XecnGV group of betabaculoviruses, though what role the products of these ORFs play in the unusual features of their pathology remains to be determined. PCR analysis of cadavers from bioassays of AugaGV and EuunGV indicated that these viruses are able to infect and replicate in larvae of Helicoverpa zea and Spodoptera frugiperda, species which are susceptible to HearGV.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"674-683"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial cytochrome b motif architecture predicts host-specific genomic vulnerability across vertebrate species. 线粒体细胞色素b基序结构预测宿主特异性基因组脆弱性跨越脊椎动物物种。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-17 DOI: 10.1007/s11262-026-02256-5

Chandrika Murugaiah

{"title":"Mitochondrial cytochrome b motif architecture predicts host-specific genomic vulnerability across vertebrate species.","authors":"Chandrika Murugaiah","doi":"10.1007/s11262-026-02256-5","DOIUrl":"10.1007/s11262-026-02256-5","url":null,"abstract":"Mitochondrial DNA (mtDNA) plays a pivotal role in cellular bioenergetics, immune regulation, and evolutionary adaptation, making it a uniquely informative genomic system for studying host-pathogen interactions. The cytochrome b (cyt b) gene, one of the most conserved mitochondrial protein-coding loci, is widely recognized for its utility in species identification, phylogenetics, and molecular evolution [1-3]. However, its potential role in shaping comparative host susceptibility to infectious diseases remains underexplored. In this study, we re-analyzed PCR-RFLP-derived mitochondrial cyt b restriction profiles targeting CCNNGG, GTAC, AGCT, TTAA, and CGCG motifs across seven vertebrate hosts: cattle, buffalo, pig, goat, rabbit, chicken, and quail. These motif architectures were compared with documented susceptibility patterns for Pasteurellosis, foot-and-mouth disease (FMD), Rinderpest, and Sarcoptes scabiei infestation [4, 6, 7]. The integrated motif matrix revealed distinct host-specific restriction profiles and four comparative genomic vulnerability clades. The findings support a model in which conserved mitochondrial motif architecture may reflect evolutionary genomic signatures relevant to pathogen-host range and susceptibility. The study is intended as a comparative genomic framework and does not claim direct causality between single restriction motifs and disease outcome.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"684-689"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological trends of HCV subtypes in China. 中国HCV亚型流行病学趋势

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-30 DOI: 10.1007/s11262-026-02264-5

Leihuan Sun, Leiliang Zhang

{"title":"Epidemiological trends of HCV subtypes in China.","authors":"Leihuan Sun, Leiliang Zhang","doi":"10.1007/s11262-026-02264-5","DOIUrl":"10.1007/s11262-026-02264-5","url":null,"abstract":"While China has made notable progress toward the World Health Organization's goal of hepatitis elimination, substantial challenges continue to persist. In recent years, the prevalence of hepatitis C virus (HCV) in China has remained high. Understanding the distribution and trends of HCV genotypes is crucial for effective disease management. This study systematically analyzes the distribution and changes in various HCV subtypes across China, utilizing data from 29,874 cases identified through high-quality detection methods in 123 articles published between 1994 and 2024. Our findings indicate that the five most prevalent HCV subtypes are 1b, 3b, 6a, 2a, and 3a. The 1b subtype is primarily found in Guangdong (26.71%), Heilongjiang (25.16%), Yunnan (8.42%), Guizhou (6.85%) and Hubei (5.52%). The 2a subtype is concentrated in Heilongjiang (55.85%), Guangdong (8.35%) and Yunnan (6.7%), while subtypes 3b, 3a, and 6a are more common in southern provinces such as Yunnan, Guizhou, and Guangdong. Importantly, the distribution of subtypes in Southern and Southwestern China differs significantly from other areas, with genotypes 3 and 6 comprising over 50% of cases. This study highlights the need for ongoing surveillance and targeted strategies in HCV management to adapt to the evolving landscape of viral genotypes in China.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"613-623"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148632685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between mutations in reverse transcriptase region of hepatitis B virus and progression of liver disease. 乙型肝炎病毒逆转录酶区突变与肝病进展的关系

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-28 DOI: 10.1007/s11262-026-02261-8

Fang Jin, Dongli Li, Weihua Zou, Jianfeng Zhong, Zhaowei Tong, Fuchu Qian

{"title":"The relationship between mutations in reverse transcriptase region of hepatitis B virus and progression of liver disease.","authors":"Fang Jin, Dongli Li, Weihua Zou, Jianfeng Zhong, Zhaowei Tong, Fuchu Qian","doi":"10.1007/s11262-026-02261-8","DOIUrl":"10.1007/s11262-026-02261-8","url":null,"abstract":"The specific objective of this study was to investigate mutations of Hepatitis B virus (HBV) reverse transcriptase (RT) region in Chinese HBV-infected patients at different stages and their association with the progression of liver disease. HBV RT regions in serum samples were amplified and sequenced from 384 patients with chronic HBV infection, including 100 asymptomatic carriers (ASC), 101 chronic hepatitis B, 96 liver cirrhosis, and 87 hepatocellular carcinoma (HCC). Further analysis of genotyping and AA substitution within RT was performed by the online HBV genotyping tool and MEGA7.0 software. A logistic regression model was used to explore the relationship between RT mutations and advanced liver disease (ALD). The mutation rate gradually increased from ASC to HCC (P for trend < 0.05). Logistic regression analysis showed that one RT site mutation, rtS106A/C, was significantly associated with the ALD. Multivariate regression analysis confirmed that two RT mutations, rtH55R/Q/K and rtS137T/Q/E, were independent risk factors for HBV-related HCC. The current study reveals that mutations within the RT region might be associated with the progression of HBV-related liver diseases and might serve as predictive biomarkers for patients with HBV infection at high risk of developing HCC.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"596-605"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148609566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a thermostable Newcastle disease virus D58 strain by in ovo vaccination and challenge study. 一株耐热新城疫病毒D58的蛋内接种和攻毒评价。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-06-24 DOI: 10.1007/s11262-026-02252-9

R Ranjini, Elaiyaraja Govindaraj, Anbukumar Karuppannan, S Rajalakshmi, K Shoba, K Porteen, P Raja

{"title":"Evaluation of a thermostable Newcastle disease virus D58 strain by in ovo vaccination and challenge study.","authors":"R Ranjini, Elaiyaraja Govindaraj, Anbukumar Karuppannan, S Rajalakshmi, K Shoba, K Porteen, P Raja","doi":"10.1007/s11262-026-02252-9","DOIUrl":"10.1007/s11262-026-02252-9","url":null,"abstract":"The persistent incidence of Newcastle disease (ND) in vaccinated flocks highlights the limitations of conventional vaccination and necessitates improved strategies like in ovo vaccination. This study evaluated the protective efficacy of a live, thermostable Newcastle disease virus (NDV) strain D58 (genotype II) in two groups of chicks (each n = 12), either delivered in ovo on embryonic day 18, or by intraocular vaccination in 7-day-old chicks. Two more groups (each n = 12) vaccinated through the in ovo or intraocular routes with commercial F strain were included for comparison. An unvaccinated group (n = 12) was used as a control. All groups were challenged with the virulent NDV D162 strain (genotype XIII) at 35 days post-hatch to assess cross-protective efficacy. Serological responses, clinical protection, viral shedding, cell-mediated immunity, and histopathological changes were assessed. The in ovo D58 group exhibited early seroconversion with significantly higher antibody titers by day 7 post-hatch. This group demonstrated 100% clinical protection, the lowest viral shedding, and enhanced cell-mediated immunity, evidenced by superior lymphocyte proliferation and cytokine gene expression. Histopathology revealed minimal lesions in the in ovo D58 group, contrasting with marked pathology in controls. In ovo D58 administration provides rapid protection and cross-protective efficacy against heterologous challenge.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"624-638"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148310427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and evolutionary analysis of HIV-1 among migration populations: a five-year cross-sectional molecular epidemiology study. 移民人群中HIV-1的遗传多样性和进化分析：一项为期五年的横断面分子流行病学研究。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-13 DOI: 10.1007/s11262-026-02258-3

Ying Su, Fei Sun, Peng Luo, Yang Qi, Xiaojian Zhou, Xinbin Chen, Yuxiang Huang, Haibo Wang

{"title":"Genetic diversity and evolutionary analysis of HIV-1 among migration populations: a five-year cross-sectional molecular epidemiology study.","authors":"Ying Su, Fei Sun, Peng Luo, Yang Qi, Xiaojian Zhou, Xinbin Chen, Yuxiang Huang, Haibo Wang","doi":"10.1007/s11262-026-02258-3","DOIUrl":"10.1007/s11262-026-02258-3","url":null,"abstract":"It is crucial to conduct strict surveillance on the diversity of HIV-1 to aid the generation of evidence-based biomedical and behavioral prevention programs to HIV/AIDS. In the current study, the social demographic characteristics, HIV-1 viral diversity among migration populations between 2017 and 2021 were investigated comprehensively and were compared with those in the previous five-year, between 2012 and 2016. Epidemiological investigation indicated that the male-to-female ratio decreased from 10.34:1 to 5.12:1, indicating significantly more female were infected when compared to the previous five years (p = 0.004). Moreover, an inversion on both the gender and nationality was found when comparison between study participants from southeastern ports and northwestern ports (p < 0.05) during the ten-year study period. Molecular epidemiology study revealed that two subtypes C/BC and CRF01_AE dominated, with the emerging of novel unique recombinant forms, including CRF01_AE/C/BC, CRF03_AB/C/BC, A/D, CRF01_AE/C, CRF08_BC/B, CRF01_AE/B, and B/CRF01_AE/C between 2017 and 2021. In addition, a significant higher proportion of drug-resistant viruses was found among this population when compared with the previous five-year (p < 0.001). In conclusion, the altered social demographic characteristics, co-circulation of CRF01_AE, C/BC recombinants, multiple URFs, and increasing amount of multiple drug-resistant viruses, indicated a new trend of the HIV-1 molecular epidemic in this population, which called for enhanced public health responses and prioritization of resources.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"576-586"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148431402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular evidence of two begomoviruses and a novel DNA-B infecting Duranta erecta variegata in Pakistan. 巴基斯坦两种begomovirus和一种新型DNA-B感染Duranta erecta varegata的分子证据。

IF 2 4区医学

Virus Genes Pub Date : 2026-10-01 Epub Date: 2026-07-22 DOI: 10.1007/s11262-026-02255-6

Afzal Akram, Mahnoor Jamil, Imran Amin, Jodi A Scheffler, Brian E Scheffler, Muhammad Saeed, Shahid Mansoor

{"title":"Molecular evidence of two begomoviruses and a novel DNA-B infecting Duranta erecta variegata in Pakistan.","authors":"Afzal Akram, Mahnoor Jamil, Imran Amin, Jodi A Scheffler, Brian E Scheffler, Muhammad Saeed, Shahid Mansoor","doi":"10.1007/s11262-026-02255-6","DOIUrl":"10.1007/s11262-026-02255-6","url":null,"abstract":"Rapidly evolving begomoviruses are a serious threat to cash crops worldwide. Perennial plants often harbor these viruses for a long time, making them accessible to crop plants. Perennial ornamental plants Duranta erecta variegata (Variegated Sky Flower) were found with typical begomoviral symptoms, including leaf curling, leaf deformation, and leaf shortening in the plant nurseries of Faisalabad, Pakistan, in 2021. Begomovirus and its associated satellite molecules of ~ 2.8 and ~ 1.4 kb, respectively, were amplified using rolling circle amplification (RCA). Sanger sequencing of cloned molecules revealed the presence of rose leaf curl virus (RoLCuV, Begomovirus rosae) accompanied by a novel recombinant DNA-B, tentatively named \"rose leaf curl virus DNA-B,\" along with hollyhock yellow vein alphasatellite (HYVA, Gosmusatellite alceae). Another plant sample showed the presence of agriculturally important papaya leaf crumple virus (PaLCrV, Begomovirus papayae). To our knowledge, this is the first report of RoLCuV, a novel DNA-B, PaLCrV, and HYVA infecting D. erecta variegata in Pakistan.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"690-694"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational identification of conserved B- and T-cell epitopes for multi-epitope peptide vaccine design against bovine leukemia virus. 牛白血病病毒多表位肽疫苗设计中保守B和t细胞表位的计算鉴定。

IF 2 4区医学

Virus Genes Pub Date : 2026-09-30 DOI: 10.1007/s11262-026-02279-y

S Sevinc Temizkan, S Kökkaya, M C Temizkan

{"title":"Computational identification of conserved B- and T-cell epitopes for multi-epitope peptide vaccine design against bovine leukemia virus.","authors":"S Sevinc Temizkan, S Kökkaya, M C Temizkan","doi":"10.1007/s11262-026-02279-y","DOIUrl":"https://doi.org/10.1007/s11262-026-02279-y","url":null,"abstract":"Bovine leukosis continues to impose significant economic losses on the global cattle industry due to its persistent infection and widespread prevalence worldwide. Although control strategies such as test-and-cull programs have been implemented in some countries, an effective and safe vaccine remains unavailable. In this study, an immunoinformatics-based approach combined with reverse vaccinology was applied to design a multi-epitope peptide (MEP) vaccine candidate against Bovine leukemia virus (BLV). Conserved protein regions were analyzed to identify potential immune targets, resulting in the selection of five epitopes, including three cytotoxic T-lymphocyte (CTL) epitopes, one helper T-lymphocyte (Th) epitope, and one linear B-cell epitope derived from the highly conserved BLV Gag and RT-IN proteins. These epitopes were assembled using AAY and GPGPG linkers to generate a 67-amino-acid MEP construct. In silico evaluation indicated that the designed construct possesses favorable immunological properties, including high predicted antigenicity (VaxiJen score: 0.6511, which is notably higher than the baseline score of 0.4784 for the native viral proteins; Pr44: 0.4784, RT-IN: 0.4214), non-allergenic characteristics, and absence of predicted toxic motifs. Homology analysis against the Bos taurus proteome revealed no significant similarity, suggesting a low risk of host cross-reactivity. Structural analyses using PSIPRED and AlphaFold 3 predicted localized secondary structural elements within an overall flexible peptide architecture; this flexibility is highly advantageous for optimal proteasomal cleavage, efficient antigen processing, and MHC presentation. Taken together, these findings provide a rational framework for the development of a BLV MEP vaccine candidate and support further experimental validation to evaluate its predicted immunogenic potential.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149118145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0