Virus Genes最新文献_第2页

Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway. 猪流行性腹泻病毒 E 蛋白通过激活 PERK 和 ATF6 而非 IRE1 信号通路诱导未折叠蛋白反应。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s11262-024-02108-0

Liang Zheng, Ying Yang, Mingxin Ma, Qin Hu, Zhijun Wu, Matthew Kay, Xiaoge Yang, Liwei Yin, Fusheng Ding, Hua Zhang

{"title":"Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.","authors":"Liang Zheng, Ying Yang, Mingxin Ma, Qin Hu, Zhijun Wu, Matthew Kay, Xiaoge Yang, Liwei Yin, Fusheng Ding, Hua Zhang","doi":"10.1007/s11262-024-02108-0","DOIUrl":"10.1007/s11262-024-02108-0","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR). However, how UPR is directly regulated by PEDV E protein remains elusive. Thus, in this study, we investigated the expression of ER chaperone glucose-regulated protein 78 (GRP78) and activations of the three main UPR signaling pathways to elucidate the underlying mechanisms of UPR triggered by PEDV E protein. The results showed that over-expression of PEDV E protein increased expression of GRP78 and induced stronger phosphorylation of both protein kinase RNA-like ER kinase (PERK) and eukaryotic initiation factor-2α (eIF2α), as well as caused the significant degradation of activating transcription factor 6 (ATF6), in both dose- and time-dependent manners. However, PEDV E protein did not induce UPR through the inositol-requiring enzyme 1 (IRE1) signaling pathway, as revealed by the splicing of XBP1 remaining unaffected and unchanged when PEDV E protein was overexpressed. Taken together, these results demonstrate that PEDV E protein induces UPR through activation of both PERK and ATF6 pathways rather than IRE1 signaling. This study not only provides mechanistic details of UPR induced by the PEDV E protein, but also provides insights into these new biologic functions to help us better understand the interactions between PEDV and host cells.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"652-666"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The conserved cysteines at position 18, 36, and 49 of Autographa californica multiple nucleopolyhedrovirus VP39 are essential for virus replication. 位于 Autographa californica 多核多面体病毒 VP39 第 18、36 和 49 位的保守半胱氨酸对病毒复制至关重要。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-10-06 DOI: 10.1007/s11262-024-02111-5

Leyuan Zhu, Lixia Xu, Wangtai Luo, Qingying Lai, Zhenqiu Huang, Meijin Yuan, Wenbi Wu, Kai Yang

{"title":"The conserved cysteines at position 18, 36, and 49 of Autographa californica multiple nucleopolyhedrovirus VP39 are essential for virus replication.","authors":"Leyuan Zhu, Lixia Xu, Wangtai Luo, Qingying Lai, Zhenqiu Huang, Meijin Yuan, Wenbi Wu, Kai Yang","doi":"10.1007/s11262-024-02111-5","DOIUrl":"10.1007/s11262-024-02111-5","url":null,"abstract":"Autographa californica nucleopolyhedrovirus orf89 (vp39) encodes the major capsid protein VP39. Multiple alignments of protein sequences showed that VP39 has 8 conserved cysteine (Cys) residues. Cysteine residues play an important role in proper function of a protein. To determine the importance of these conserved cysteine residues for virus proliferation, a series of recombinant viruses harboring VP39-Cys mutants were constructed. Viral growth curves and transmission electron microscopy showed that mutation of Cys29, Cys132, Cys169, Cys229, or Cys232 of VP39 to alanine did not affect budded virion production; however, the mutation of Cys18, Cys36, or Cys49 to alanine resulted in interruption of capsid assembly. Co-immunoprecipitation assays showed that mutations of these 8 cysteines individually or simultaneously had no effect on self-association of VP39. Immunofluorescence analysis by confocal microscopy revealed that the subcellular localization of VP39 with mutations in Cys18, Cys36 or Cys49 was exclusively distributed in the cytoplasm of a cell regardless of virus infection or not, while the wild-type VP39 or the VP39 carrying mutations in Cys29, Cys132, Cys169, Cys229, or Cys232 was distributed throughout the cytoplasm and the nucleus. Our results demonstrated that Cys18, Cys36, and Cys49 are essential for the proper localization of VP39, which is a prerequisite for successful nucleocapsid assembly of the virus.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"711-724"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and comparison of tomato zonate spot virus isolated in Japan and China. 分离自日本和中国的番茄带状斑点病毒的分子特征和比较。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-16 DOI: 10.1007/s11262-024-02098-z

Momoko Matsuyama, Yasuhiro Tomitaka

引用次数: 0

First reports of several viruses and a viroid including a novel vitivirus in Japan, found through virome analysis of bulk grape genetic resources. 通过对大量葡萄遗传资源进行病毒组分析，首次报告了日本的几种病毒和一种病毒，包括一种新型葡萄病毒。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-20 DOI: 10.1007/s11262-024-02101-7

Takao Ito

{"title":"First reports of several viruses and a viroid including a novel vitivirus in Japan, found through virome analysis of bulk grape genetic resources.","authors":"Takao Ito","doi":"10.1007/s11262-024-02101-7","DOIUrl":"10.1007/s11262-024-02101-7","url":null,"abstract":"Virome analysis was performed on 174 grape genetic resources from the National Agriculture and Food Research Organization, Japan. A total of 20 bulk samples was prepared by grouping the vines into batches of 6-10 plants. Each of the bulk samples was analyzed using high-throughput sequencing, which detected 27 viruses and 5 viroids, including six viruses and one viroid reported in Japan for the first time (grapevine viruses F, L, and T, grapevine Kizil Sapak virus, grapevine Syrah virus 1, grapevine satellite virus, and grapevine yellow speckle viroid 2). In addition, a novel vitivirus was detected with a maximum nucleotide sequence identity of only 58% to its closest relative, grapevine virus A (GVA). The genome of this novel virus was 7,461 nucleotides in length and encoded five open reading frames showing the typical genomic structure of vitiviruses. Phylogenetic trees of vitiviruses placed it in a distinct position nearest to GVA or grapevine virus F (GVF) in genomes and amino acids of deduced replication-associated protein (RAP) and coat protein (CP). The amino acid sequence identities of RAP and CP with GVA, GVF, and other vitiviruses were a maximum of 53% and 73%, respectively, which were significantly below the species demarcation threshold of 80% in the genus. The low identity and phylogenetic analyses indicate the discovery of a novel vitivirus species provisionally named grapevine virus P.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"684-694"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142005797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomics and evolutionary analysis of dengue virus strains circulating in Pakistan. 巴基斯坦流行的登革热病毒株系的比较基因组学和进化分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-28 DOI: 10.1007/s11262-024-02100-8

Aziz Ul-Rahman, Muhammad Zubair Shabbir, Majeeda Rasheed, Nusrat Shafi, Kalsoom AbdulRazaq, Hamna Ramzan, Rauf Mehmood, Junaid Ali Khan

{"title":"Comparative genomics and evolutionary analysis of dengue virus strains circulating in Pakistan.","authors":"Aziz Ul-Rahman, Muhammad Zubair Shabbir, Majeeda Rasheed, Nusrat Shafi, Kalsoom AbdulRazaq, Hamna Ramzan, Rauf Mehmood, Junaid Ali Khan","doi":"10.1007/s11262-024-02100-8","DOIUrl":"10.1007/s11262-024-02100-8","url":null,"abstract":"Dengue fever virus (DENV) poses a significant public health risk in tropical and subtropical regions across the world. Although the dengue fever virus (DENV) exhibits significant genetic diversity and has the potential to evolve, there is a lack of comprehensive research on the comparative genomics and evolutionary dynamics of the virus in Pakistan. Phylogenetic analysis demonstrated the circulation of all four dengue virus serotypes (DENV-1, - 2, - 3, and - 4) with prevalent genotypes III and V within DENV-1, cosmopolitan genotype within DENV-2, genotype III within DENV-3, and genotype I within DENV-4 during 2006-2014. Based on the complete envelope region, genome-wide residue signature and genetic diversity indicate that there is a high level of genetic diversity among DENV-1 strains, while DENV-3 strains exhibit the least genetic diversity. Comparative analysis of all four DENV serotypes revealed that certain codons in DENV-2 and -4 were subject to strong purifying selection, while a few codon sites in the envelope region showed evidence of positive selection. These findings provided valuable insights into the comparative genomics and evolutionary pattern of DENV strains reported from Pakistan. Whether those characteristics conferred a fitness advantage to DENV-1 genotypes within a specific geography and time interval warrants further investigations. The findings of the current study will contribute to tracking disease dynamics, understanding virus transmission and evolution, and formulating effective disease control strategies.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"603-620"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142094080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new isolate of mungbean yellow mosaic India virus in Vigna mungo L. reported from a Dayalbagh field, Agra. 从阿格拉的 Dayalbagh 农田中报告了绿豆黄镶嵌印度病毒的新分离株。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-21 DOI: 10.1007/s11262-024-02099-y

Ambika Chaturvedi, Dipinte Gupta, Bikash Mandal, Rajiv Ranjan

{"title":"A new isolate of mungbean yellow mosaic India virus in Vigna mungo L. reported from a Dayalbagh field, Agra.","authors":"Ambika Chaturvedi, Dipinte Gupta, Bikash Mandal, Rajiv Ranjan","doi":"10.1007/s11262-024-02099-y","DOIUrl":"10.1007/s11262-024-02099-y","url":null,"abstract":"Black gram (Vigna mungo L.) plants showing yellow mosaic symptoms during 2019-2022 crop seasons were collected randomly from a Dayalbagh field, Agra Region of Uttar Pradesh, India. Total genomic DNA was isolated from the infected leaf samples by the Cetyltrimethylammonium bromide (CTAB) method and subjected to PCR. After viral confirmation, the viral genome was amplified by rolling circle amplification following the standard protocol. The DNA A and DNA B subgenomes were cloned individually as a PstI and BamHI fragment in the pUC18 vector. Positive clones were subjected to DNA sequencing. The results revealed that DNA A and DNA B show the closest nucleotide identity with \"mungbean yellow mosaic India virus-[Mungbean], DNA-A, the complete sequence\" (GeneBank Accession No AF416742.1) with 98.14% identity, and \"mungbean yellow mosaic India virus isolate Mu1-Dholi segment DNA-B, the complete sequence\" (GeneBank Accession No MW814723.1) with 97.94% identity, respectively. The new isolate of mungbean yellow mosaic India virus (MYMIV) shows sequence similarity with the coat protein gene of various strains of MYMIV. In the new isolate of MYMIV, a point mutation was observed at the 2036th nucleotide of DNA B, which disrupts the reading frame to introduce a stop codon and thus leading to a decrease in the size of the movement protein gene. In the present study we are reporting the whole genome sequence of the MYMIV Dayalbagh isolate for the first time.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"747-751"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of mycoviruses in transcriptomes from the fungal family ceratocystidaceae. 鉴定和表征陶瓷囊菌科真菌转录组中的霉菌病毒。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-10-08 DOI: 10.1007/s11262-024-02112-4

Bianca Hough, Brenda Wingfield, David Read

{"title":"Identification and characterization of mycoviruses in transcriptomes from the fungal family ceratocystidaceae.","authors":"Bianca Hough, Brenda Wingfield, David Read","doi":"10.1007/s11262-024-02112-4","DOIUrl":"10.1007/s11262-024-02112-4","url":null,"abstract":"Mycoviruses pervade the fungal kingdom, yet their diversity within various fungal families and genera remains largely unexplored. In this study, 10 publicly available fungal transcriptomes from Ceratocystidaceae were analyzed for the presence of mycoviruses. Despite mycovirus associations being known in only four members of this family, our investigation unveiled the discovery of six novel mycoviruses. The majority of these mycoviruses are composed of positive sense single stranded RNA and are putatively assigned to the viral family Mitoviridae (with tentative classification into the genera Unuamitovirus and Duamitovirus). The double stranded RNA viruses, however, were associated with the family Totiviridae (with tentative classification into the genus Victorivirus). This study also revealed the discovery of an identical unuamitovirus in the fungal species Thielaviopsis ethacetica and Thielaviopsis paradoxa. This discovery was notable as these fungal isolates originated from distinct geographical locations, highlighting potential implications for the transmission of this mitovirus. Moreover, this investigation significantly expands the known host range for mycoviruses in this family, marking the initial identification of mycoviruses within Ceratocystis platani, Thielaviopsis paradoxa, Thielaviopsis ethacetica, and Huntiella omanensis. Future research should focus on determining the effects that these mycoviruses might have on their fungal hosts.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"696-710"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines. 利用 YBX3 基因敲除和过表达细胞系，探索 YBX3 在 PEDV 感染中的作用。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s11262-024-02109-z

Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang

{"title":"Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.","authors":"Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang","doi":"10.1007/s11262-024-02109-z","DOIUrl":"10.1007/s11262-024-02109-z","url":null,"abstract":"Porcine epidemic diarrhea (PED) is a highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV), which results in significant economic losses. PEDV infection causes severe damage to the midgut barrier in the small intestine. YBX3, an important protein in tight junctions, promotes epithelial cell proliferation. However, its role in the process of PEDV infection remains unclear. In this study, we observed a significant increase in mRNA expression of YBX3 following PEDV infection. Additionally, the protein expression of YBX3 showed an initial increase followed by a decrease over time. Furthermore, treatment with 2% DSS resulted in a significant down-regulation of YBX3 mRNA and protein expression. Furthermore, we successfully generated knockout and overexpression cell lines of YBX3. Preliminary assays indicated that elevated expression of YBX3 inhibited the PEDV replication, while knockout of YBX3 had the opposite effect. In conclusion, our study has preliminarily revealed the functional role of YBX3 during PEDV infection. This finding lays the foundation for further investigation into its mechanism in future and also provides new insights into the mechanism of PEDV-host interactions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"667-673"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of the novel phage SAP71 and its potential use against Staphylococcus aureus in an atopic dermatitis mouse model. 新型噬菌体 SAP71 的分离及其在特应性皮炎小鼠模型中对抗金黄色葡萄球菌的潜力。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s11262-024-02106-2

Huaixin Geng, Xin Yang, Chenghui Zou, Wen Zhang, Jingheng Xiang, Kailang Yang, Yi Shu, Guangxin Luan, Xu Jia, Mao Lu

引用次数: 0

Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen. 表达orf病毒的疫苗病毒H3L跨膜蛋白类似物F1L，并将其成功纯化为诊断抗原。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-13 DOI: 10.1007/s11262-024-02097-0

Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal

{"title":"Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen.","authors":"Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal","doi":"10.1007/s11262-024-02097-0","DOIUrl":"10.1007/s11262-024-02097-0","url":null,"abstract":"Orf or contagious ecthyma is a highly contagious, zoonotic, and economically important global viral disease of small ruminants and is endemic in India. Vaccination of susceptible goats/sheep along with suitable recombinant protein-based serological assay will be useful in the control of the infection. In this study, the full-length and truncated versions of F1L encoding gene (ORF 059) of orf virus were cloned into pFasBac HT A vector, transformed in DH10Bac cells, and expressed in insect cells. The full-length and truncated recombinant F1L proteins were expressed as a 6 × histidine-tagged fusion protein for ease of purification by Ni-NTA affinity chromatography under denaturing conditions. A protein with ~ 40 kDa and ~ 35 kDa for full-length and truncated F1L protein, respectively, were expressed and confirmed by SDS-PAGE and western blot. The protein reactivity evaluated by western blot analysis and indirect ELISA using ORFV hyperimmune serum was also found to be reactive. The results of the present study showed that the purified recombinant F1L protein can be used as a diagnostic antigen in sero-surveillance of ORFV infection in small ruminants. To the best of authors' knowledge, this is the first report on the expression of ORFV F1L in insect cells using a baculovirus vector and its successful purification to use as the potential diagnostic antigen in ELISA.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"642-651"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0