Virus Genes最新文献_第2页

Isolation and identification of avian leukosis virus subgroup J from Luohé Ma chicken. 鸡白血病病毒J亚群的分离与鉴定。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-07-08 DOI: 10.1007/s11262-025-02174-y

Ningning Yang, Chuangfu Chen, Mingguo Xu, Guizhi Zhang

{"title":"Isolation and identification of avian leukosis virus subgroup J from Luohé Ma chicken.","authors":"Ningning Yang, Chuangfu Chen, Mingguo Xu, Guizhi Zhang","doi":"10.1007/s11262-025-02174-y","DOIUrl":"10.1007/s11262-025-02174-y","url":null,"abstract":"Henan Province is rich in livestock and poultry resources, home to well-known local breeds, including Gushi, Lushi, and Luohé Ma chickens. These breeds are of substantial agricultural and economic importance and play a pivotal role in preserving biodiversity as well as fostering genetic innovation. Avian leukosis (AL) is a major viral disease affecting the poultry industry, with the J subgroup of avian leukosis virus (ALV-J) emerging as a significant cause of economic losses. In this study, we performed reverse transcription -polymerase chain reaction (RT-PCR) identification on chickens suspected of ALV infection based on clinical symptoms and pathological changes in the parent stock breeder farm of Luohé Ma chicken in Henan Province, and isolated the virus from the samples initially identified as positive. Indirect enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescent antibody (IFA), transmission electron microscopy (TEM), and phylogenetic tree analysis were used to further determine whether ALV infection existed and its infection subtype. The results demonstrated that ALV infection did exist in the breeding farm of the Luohé chicken parents, and we successfully isolated and obtained an ALV strain, which was named HN-1. The RT-PCR results showed that only the gp85 gene sequence of the ALV-J subtype was amplified, and the nucleotide sequence was submitted to GenBank, where it was assigned the accession number PQ010741.1. Its genetic evolutionary relationship was most closely related to the ALV-J subtype strain FJ201307. In conclusion, our findings indicate the presence of ALV-J infection in this chicken farm and remind us that appropriate measures should be taken before ALV-J outbreaks to minimize economic losses.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"588-595"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARF3 knockdown inhibits influenza a virus and virus-induced pneumonia. ARF3敲除抑制甲型流感病毒和病毒诱导的肺炎。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-07-03 DOI: 10.1007/s11262-025-02173-z

Zhinan Zhang, Banghao Lu, Bihe Zeng

引用次数: 0

Detection of potato virus S in Colorado potato beetle RNA-seq data. 科罗拉多马铃薯甲虫中马铃薯S病毒的RNA-seq检测。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-07-30 DOI: 10.1007/s11262-025-02179-7

Maria Antonets, Sergei Bodnev, Ulyana Rotskaya, Vadim Kryukov, Denis Antonets

{"title":"Detection of potato virus S in Colorado potato beetle RNA-seq data.","authors":"Maria Antonets, Sergei Bodnev, Ulyana Rotskaya, Vadim Kryukov, Denis Antonets","doi":"10.1007/s11262-025-02179-7","DOIUrl":"10.1007/s11262-025-02179-7","url":null,"abstract":"Potato virus S (PVS) is a widely distributed potato pathogen that typically causes mild or asymptomatic infections. Still, certain genotypes can cause up to 20% yield losses, especially when co-infected with other viruses. Aphids are the only currently recognized insect vectors of PVS; however, the mechanical transmission through plant contact is also known. The Colorado potato beetle (CPB) is a major potato pest. In this study, we report the partial PVS genome assembled from the RNA-seq data obtained from CPB field samples and the phylogenetic analysis of its triple gene block (TGB) nucleotide sequence with corresponding sequences, extracted from published PVS genomes and PVS genomes assembled from publicly available CPB RNA-Seq data. The CPB-derived PVS sequences clustered within the globally prevalent PVSI phylogroup, indicating they are typical PVS strains rather than a highly divergent variant. These results demonstrate the wide presence of PVS genetic material in CPB. With the emerging PVS variants showing increased transmissibility and pathogenicity, PVS could be considered an underappreciated potato pathogen and thus further studies are needed to determine whether CPB may play a role in PVS transmission.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"596-602"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raising Awareness About Compromised Cell Lines, Better Science Practices, and Accountability. 提高对受损细胞系的认识，更好的科学实践和问责制。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-07-31 DOI: 10.1007/s11262-025-02180-0

Deilson Elgui de Oliveira, Maiara Venancio de Oliveira, Patricia de Cassia de Morais Pereira-Sanches

{"title":"Raising Awareness About Compromised Cell Lines, Better Science Practices, and Accountability.","authors":"Deilson Elgui de Oliveira, Maiara Venancio de Oliveira, Patricia de Cassia de Morais Pereira-Sanches","doi":"10.1007/s11262-025-02180-0","DOIUrl":"10.1007/s11262-025-02180-0","url":null,"abstract":"This comment raises concerns about the validity and reproducibility of a publication by Li et al. in Virus Genes (60:488-500, 2024. https://doi.org/10.1007/s11262-024-02096-1 ; PMID: 39103702), which aimed to investigate the role of aquaporin-3 in nasopharyngeal carcinoma (NPC) and its regulation by the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1). The central criticism focuses on using the CNE-1, CNE-2, and HONE-1 cell lines, which are recognized as unreliable for NPC research. There is an alarming prevalence of publications that continue to utilize these compromised cell lines, impeding genuine advancements in understanding NPC. Beyond the cell line authenticity issue, the study lacks relevant methodological information and justification for experimental design choices. We emphasize the need for raw data availability and comprehensive methodological information to ensure reproducibility. Ultimately, this comment calls for a more rigorous and multi-faceted approach to quality control in scientific publishing and embracing Open Science principles to ensure the reliability of published research.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"544-546"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights of HIV-1 and HBV transcriptional regulation by viral transactivator Tat and HBx. 病毒反激活子Tat和HBx对HIV-1和HBV转录调控的见解。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-08-12 DOI: 10.1007/s11262-025-02176-w

Zhonglan Wu, Jianxin Pei, Yong Li

引用次数: 0

Correction: Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study. 更正：在考拉感染多种考拉逆转录病毒亚型后，癌基因表达升高：初步研究。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 DOI: 10.1007/s11262-025-02181-z

Lipi Akter, Md Abul Hashem, Mohammad Enamul Hoque Kayesh, Tofazzal Md Rakib, Md Haroon Or Rashid, Fumie Maetani, Kyoko Tsukiyama-Kohara

引用次数: 0

Association of RBD mutations with COVID-19 disease severity in the Iranian population. 伊朗人群中RBD突变与COVID-19疾病严重程度的关系

IF 1.9 4区医学

Virus Genes Pub Date : 2025-10-01 Epub Date: 2025-06-26 DOI: 10.1007/s11262-025-02168-w

Mozhgan Mondeali, Mohamad Mahjoor, Mansoor Khaledi, Ahdiyeh Saghabashi, Seyedeh Faride Alavi Rostami, Mohammad Hossein Modarressi

{"title":"Association of RBD mutations with COVID-19 disease severity in the Iranian population.","authors":"Mozhgan Mondeali, Mohamad Mahjoor, Mansoor Khaledi, Ahdiyeh Saghabashi, Seyedeh Faride Alavi Rostami, Mohammad Hossein Modarressi","doi":"10.1007/s11262-025-02168-w","DOIUrl":"10.1007/s11262-025-02168-w","url":null,"abstract":"The global public health is still at risk due to the COVID-19 pandemic, which was caused by SARS-CoV-2. Disease severity varies among patients and is influenced by mutations in the viral genome, particularly within the spike protein's receptor-binding domain (RBD). This study aimed to investigate the association between RBD mutations and disease severity and to shed light on the fundamental molecular mechanisms. Nasopharyngeal and oropharyngeal samples were obtained from 70 COVID-19 patients in Iran, including 35 mild and 35 deceased cases. The RBD region of the spike protein gene underwent amplification through reverse transcription-polymerase chain reaction (RT-PCR) and was subsequently sequenced using Sanger sequencing. The impact of RBD mutations on binding affinity to human ACE2 (hACE2) was assessed by molecular docking analyses. Sequence analysis identified seven nonsynonymous mutations within the RBD region. The N501Y mutation, which was the most prevalent, showed a significant correlation with disease severity. Molecular docking revealed that the N501Y substitution enhanced binding affinity to hACE2 by increasing hydrophobic interactions and altering the interaction patterns of neighboring residues. This study demonstrates that the N501Y mutation has an independent association with increased severity of COVID-19, likely due to its effect on strengthening the RBD-hACE2 interaction. Further studies involving larger cohorts and diverse populations are necessary to confirm these results and to explore their potential implications for disease management and therapeutic strategies.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"547-553"},"PeriodicalIF":1.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and genome analysis of Yunxiao leafhopper virus 1 in Recilia dorsalis. 背蝇云霄叶蝉病毒1型的发现及基因组分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-09-25 DOI: 10.1007/s11262-025-02186-8

Muqmirah Naseem, Bozhong Li, Guangming Xiao, Zhongtian Xu, Taiyun Wei, Hui Wang

{"title":"Discovery and genome analysis of Yunxiao leafhopper virus 1 in Recilia dorsalis.","authors":"Muqmirah Naseem, Bozhong Li, Guangming Xiao, Zhongtian Xu, Taiyun Wei, Hui Wang","doi":"10.1007/s11262-025-02186-8","DOIUrl":"https://doi.org/10.1007/s11262-025-02186-8","url":null,"abstract":"In this study, we report the discovery of a novel virus, Yunxiao leafhopper virus 1 (YXLeV1), found in the insect vector Recilia dorsalis, a significant pest of rice crop. The complete genome of YXLeV1, consisting of 14,115 bp, was sequenced and analyzed. The whole viral genome shares only 36.32% identity with the RNA-dependent RNA polymerase (RdRp) of Hubei diptera virus 11, belonging to genus Alasvirus. It contains four open reading frames encoding a nucleoprotein (N), a hypothetical protein (p78) of unknown function, a glycoprotein (G), and an RNA-dependent RdRp. The N, G, and RdRp proteins of YXLeV1 share 22.0%, 34.05%, and 36.32% amino acid sequence identity with the corresponding sequence of Hubei diptera virus 11. As per the genus demarcation criteria of the family Xinmoviridae, viruses sharing less than 60% amino acid identity in the RdRp sequence with known members are considered to belong to new genera, so the observed 36.32% identity between YXLeV1 and Hubei diptera virus 11 supports the classification of YXLeV1 as the first member of a novel genus, which we propose to name Recilivirus. Phylogenetic analysis further confirms that YXLeV1 is distantly related to Hubei diptera virus 11, the sole member of the genus Alasvirus, and forms a separate clade supporting its classification as a member of a new genus. Given the ecological significance of R. dorsalis as a vector, this discovery adds to the catalog of viruses associated with this species and contributes to our understanding of virus vector associations.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel orbivirus in Amblyomma tholloni ticks parasitizing African savanna elephants (Loxodonta africana) in Zambia. 寄生在赞比亚非洲大草原象（Loxodonta africana）上的索loni双盲蜱的新型轨道病毒。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-09-22 DOI: 10.1007/s11262-025-02187-7

Daniella E Chusyd, Lisa Olivier, Moses Kasongo, Webster Mwaanga, Tony L Goldberg

{"title":"Novel orbivirus in Amblyomma tholloni ticks parasitizing African savanna elephants (Loxodonta africana) in Zambia.","authors":"Daniella E Chusyd, Lisa Olivier, Moses Kasongo, Webster Mwaanga, Tony L Goldberg","doi":"10.1007/s11262-025-02187-7","DOIUrl":"https://doi.org/10.1007/s11262-025-02187-7","url":null,"abstract":"Orbiviruses are emerging pathogens of public and veterinary health concern. This study reports the discovery and genomic characterization of a novel orbivirus in adult ticks collected from African savanna elephants (Loxodonta africana) in Kafue National Park, Zambia. Six ticks were identified through genetic analyses; one Rhipicephalus maculatus and five Amblyomma tholloni. Salivary gland and blood meal samples were dissected for virus characterization. Total nucleic acids were extracted and reverse-transcribed, and libraries were prepared for deep sequencing. De novo assembly of trimmed sequences recovered four novel viruses designated \"zaloxo\" to indicate their association with Zambian elephants (genus Loxodonta), abbreviated ZXLV-1 to ZXLV-4. ZXLV-1, a member of the genus Orbivirus, belongs to the same subclade of dsRNA orbiviruses as bluetongue virus, epizootic hemorrhagic disease virus, and African horse sickness virus. ZXLV-2 and ZXLV-3 are most closely related to dsRNA Totiviridae and ssDNA Circoviridae viruses, respectively. ZXLV-4 belongs to the genus Alphapolyomavirus, dsDNA viruses which are ubiquitous in mammals but have unclear associations with disease. While the pathogenicity of these viruses remains unclear, these results suggest that a hidden diversity of viruses likely exists in elephant ectoparasites, warranting attention from the perspective of both health and conservation.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full-genome sequence of a novel potyvirus infecting Sauropus androgynus. 一种感染雌雄同体蜥脚类动物的新型疱疹病毒的全基因组序列。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-09-20 DOI: 10.1007/s11262-025-02185-9

Li-Juan Zhu, Shulian Su, Jingke Li, Yubin Chi, Yankun Zhu, Xing Chen, Lan-Yi Su, Juncheng Zhang, Zhongtian Xu

{"title":"Full-genome sequence of a novel potyvirus infecting Sauropus androgynus.","authors":"Li-Juan Zhu, Shulian Su, Jingke Li, Yubin Chi, Yankun Zhu, Xing Chen, Lan-Yi Su, Juncheng Zhang, Zhongtian Xu","doi":"10.1007/s11262-025-02185-9","DOIUrl":"https://doi.org/10.1007/s11262-025-02185-9","url":null,"abstract":"In the present study, the full-genome sequence of a novel potyvirus, provisionally named \"Sauropus androgynus potyvirus 1\" (SAPV1), was determined using a combination of high-throughput sequencing (HTS) contig assembly, reverse transcription polymerase chain reaction (RT-PCR), and rapid amplification of cDNA ends (RACE) PCR. The full-genome sequencing of SAPV1, excluding the 3' poly(A) tail, was 10,365 nucleotides long and encoded a large polyprotein comprising 3,315 amino acids. Maximum likelihood phylogenetic analysis based on the multiple sequence alignment of the polyprotein sequence revealed that SAPV1 clustered with the genus Potyvirus as a monophyletic clade, with its closest evolutionary relative being the Plum pox virus (PPV). BLAST searches revealed that the polyprotein sequence of SAPV1 shares the highest amino acid sequence identity of 45.6% with known viruses, with the highest being PPV. According to the species demarcation criteria of the family Potyviridae and the phylogenetic analysis, we propose that SAPV1 represents a novel member of the genus Potyvirus, infecting Sauropus androgynus, a plant widely used in medicine and the food industry.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0