Virus Genes最新文献_第3页

Prevalence of Epstein-Barr virus and JC virus in tissue samples of gastric cancer, non-malignant, and controls by polymerase chain reaction in northwest Iran. 聚合酶链反应检测伊朗西北部地区胃癌、非恶性和对照组织标本中eb病毒和JC病毒的流行情况

IF 1.9 4区医学

Virus Genes Pub Date : 2025-05-28 DOI: 10.1007/s11262-025-02165-z

Abolfazl Jafari-Sales, Afsoon Shariat, Hossein Bannazadeh-Baghi, Behzad Baradaran, Behboud Jafari

{"title":"Prevalence of Epstein-Barr virus and JC virus in tissue samples of gastric cancer, non-malignant, and controls by polymerase chain reaction in northwest Iran.","authors":"Abolfazl Jafari-Sales, Afsoon Shariat, Hossein Bannazadeh-Baghi, Behzad Baradaran, Behboud Jafari","doi":"10.1007/s11262-025-02165-z","DOIUrl":"https://doi.org/10.1007/s11262-025-02165-z","url":null,"abstract":"Gastric cancer (GC) ranks as the fourth most common cancer worldwide and is a leading cause of cancer-related deaths globally. The development of GC is influenced by multiple factors. This study aimed to detect the presence of Epstein-Barr virus (EBV) and JC virus (JCV) in cancerous, non-malignant, and control tissue samples using polymerase chain reaction (PCR) methodology. In this descriptive cross-sectional study, we analyzed 150 paraffin-embedded tissue samples collected over a seven-month period from laboratory archives in East Azerbaijan province. The samples comprised three groups: GC tissues (n = 50), non-malignant gastric tissues (n = 50), and control tissues (n = 50). PCR was performed to detect EBV and JCV. Then, Southern blot analysis was performed for EBV and JCV in PCR positive cases. Data analysis was conducted using SPSS version 18 software with chi-square testing. Among the cancer samples (mean age 61.7 ± 12.01 years), PCR analysis detected EBV in 5 samples (10%) and JCV in 2 samples (4%). The EBV-positive and JCV-positive cases had mean ages of 63.6 ± 13.31 and 61 ± 18.38 years, respectively. No viral DNA was detected in either the non-malignant or control groups. Southern blot analysis was positive in all PCR positive cases. As cancer incidence continues to rise, understanding its risk factors becomes increasingly critical. Our findings demonstrate the presence of EBV and JCV in GC tissues from this geographical region, suggesting their potential role in gastric carcinogenesis. However, the relationship between oncoviruses and GC risk remains understudied. Further research is warranted to elucidate the molecular mechanisms by which these viruses may contribute to GC development.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in treatment and detection of Rift Valley fever virus: a comprehensive overview. 裂谷热病毒治疗和检测的最新进展：全面概述。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-05-10 DOI: 10.1007/s11262-025-02164-0

Nima Afshar Moghaddam, Zahra Yekanipour, Sama Akbarzadeh, Sahar Molavi Nia, Fatemeh Abarghooi Kahaki, Mohammad Hassan Kalantar, Omid Gholizadeh

{"title":"Recent advances in treatment and detection of Rift Valley fever virus: a comprehensive overview.","authors":"Nima Afshar Moghaddam, Zahra Yekanipour, Sama Akbarzadeh, Sahar Molavi Nia, Fatemeh Abarghooi Kahaki, Mohammad Hassan Kalantar, Omid Gholizadeh","doi":"10.1007/s11262-025-02164-0","DOIUrl":"https://doi.org/10.1007/s11262-025-02164-0","url":null,"abstract":"Rift Valley fever virus (RVFV) is a newly discovered arboviral pathogen that infects humans and livestock. Numerous outbreaks have occurred in Africa and the Arab Peninsula. Epizootics of RVFV are sporadic and frequently associated with ongoing floods and excessive rainfall. This leads to the development of infected Aedes mosquitoes, which then amplify transmission by other mosquito species (like Anopheles and Culex genera). In animals, it typically results in high rates of death and abortion. In humans, Rift Valley fever (RVF) manifests as clinical symptoms that may vary in intensity from minor to severe. Common symptoms include retinitis, hepatitis, delayed onset encephalitis, and hemorrhagic illness. The possibilities for containing RVFV outbreaks are limited due to the lack of authorized human vaccinations and treatments. Although molecular detection techniques are available, they can only recognize viral nucleic acids during the short viremic phase. There are currently no specific treatments for RVFV infection. Ribavirin is one of the few therapies for viral hemorrhagic fevers, but severe adverse effects restrict its use. Significant studies have been done in recent years on using Nanotechnology to diagnose and treat viruses. This review summarizes the common and recent diagnostic and therapeutic approaches for RVFV, including nanoparticles, intravenous immunoglobulin (IVIG), stem cells, vaccines, and antibody-based therapies.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Genomic analysis of Varicella zoster virus strains during an outbreak with atypical clinical presentations in Biswanath district of Assam, India. 更正：印度阿萨姆邦比斯瓦纳特地区一次非典型临床表现暴发期间水痘带状疱疹病毒株的基因组分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-05-05 DOI: 10.1007/s11262-025-02163-1

Kimmi Sarmah, Ajanta Sharma, Kishore Sarma, Syed Tanwir Alam, Bornali Sarmah Dutta, Eliza Deka, Sahabuddin Ahmed Laskar, Narendra Singh Tishya, Manirangu Sobhana Lakshmi Priya, Achyut Chandra Baishya

引用次数: 0

Studies on insect virus-producing proteins as potential synergists for microbial insecticides: status and prospects. 昆虫产病毒蛋白作为微生物杀虫剂潜在增效剂的研究现状与展望。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-05-01 DOI: 10.1007/s11262-025-02162-2

Wataru Mitsuhashi

{"title":"Studies on insect virus-producing proteins as potential synergists for microbial insecticides: status and prospects.","authors":"Wataru Mitsuhashi","doi":"10.1007/s11262-025-02162-2","DOIUrl":"https://doi.org/10.1007/s11262-025-02162-2","url":null,"abstract":"The use of microbial insecticides in crop fields has been very limited, especially in developed countries, compared with that of synthetic (chemical) insecticides, even though the former are friendly to vertebrates (including humans and livestock), most beneficial insects, plants, and the environment. This lower use rate is attributable mainly to their more expensive commercial production and lower effectiveness compared to synthetic insecticides. The combined use of microbial insecticides and synergistic agents would strengthen the potency of these insecticides and decrease the amounts of the microbial insecticides used. This, in turn, would lead to lower costs and wider adoption. Therefore, it is important to develop an efficient method of the combined use. Natural synergists are generally less harmful to vertebrates and the environment than synthetic synergists. Here, I review recent studies on two major natural synergists derived from insect viruses: the proteins enhancin and fusolin. Enhancin originates from baculoviruses that infect insects, while fusolin is found in the insect virus group entomopoxviruses and in baculoviruses; the fusolin in baculoviruses is also referred to as GP37. In addition, I discuss prospects for the development of technologies for the use of the proteins in the fields, including the improvement of gene expression systems and genetically modified plants, and the engineering of the two proteins.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and evolutionary analysis of human adenovirus type 55, related to febrile respiratory illness in the South Korean military. 与韩国军队发热性呼吸道疾病相关的人腺病毒55型的分子特征和进化分析

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-29 DOI: 10.1007/s11262-025-02160-4

Se Hun Gu, Seung-Ho Lee, Daesang Lee, Dong Hyun Song

{"title":"Molecular characterization and evolutionary analysis of human adenovirus type 55, related to febrile respiratory illness in the South Korean military.","authors":"Se Hun Gu, Seung-Ho Lee, Daesang Lee, Dong Hyun Song","doi":"10.1007/s11262-025-02160-4","DOIUrl":"https://doi.org/10.1007/s11262-025-02160-4","url":null,"abstract":"Adenoviruses are non-enveloped, double-stranded deoxyribonucleic acid (DNA) viruses that belong to the family Adenoviridae. Human adenovirus (HAdV)-55 is associated with severe respiratory illnesses that often lead to respiratory failure and death. HAdV-55 caused a febrile respiratory illness (FRI) outbreak at a military base in the Republic of Korea. The Army Forces Capital Hospital provided DNA samples from 79 patients with FRI; among them, we obtained seven whole-genome sequences of HAdV-55 using next-generation sequencing. Phylogenetic analysis of the complete genome and penton base, hexon, and fiber gene sequences demonstrated type-specific genetic clustering among the seven HAdV-55 strains. We also demonstrated protein modeling, molecular phylogeny, and evolution based on whole-genome sequences of seven HAdV-55 isolates characterized using next-generation sequencing and bioinformatics. Additionally, HAdV-55 strains from different countries have contributed to multiple lineages and genetic evolution. Our findings provide important insights into the evolution, molecular phylogeny, protein modeling, and genome sequencing of HAdV-55 isolates. Further studies are needed to better understand the genetic variants of emerging or re-emerging HAdVs.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and genetic diversity of HTLV-1 among blood donors in Jiroft, Iran: a comprehensive study. 伊朗吉洛夫特献血者中HTLV-1的患病率和遗传多样性：一项综合研究

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-28 DOI: 10.1007/s11262-025-02159-x

Fahime Edalat, Amir Gholamzad, Zohreh-Al-Sadat Ghoreshi, Mohammad Dalfardi, Ahmad Golkar, Emad Behboudi, Nasir Arefinia

{"title":"Prevalence and genetic diversity of HTLV-1 among blood donors in Jiroft, Iran: a comprehensive study.","authors":"Fahime Edalat, Amir Gholamzad, Zohreh-Al-Sadat Ghoreshi, Mohammad Dalfardi, Ahmad Golkar, Emad Behboudi, Nasir Arefinia","doi":"10.1007/s11262-025-02159-x","DOIUrl":"https://doi.org/10.1007/s11262-025-02159-x","url":null,"abstract":"This study aimed to investigate the prevalence and phylogenetic characteristics of Human T-cell lymphotropic virus type 1 (HTLV-1) among blood donors in Jiroft Province, southeast Iran, a region previously under-studied regarding this virus. A total of 405 blood donor samples were collected from six cities within Jiroft Province. Serum samples were screened for HTLV-1 antibodies using the ELISA method, while peripheral blood mononuclear cells (PBMCs) were analyzed via PCR targeting the long terminal repeat (LTR) and TAX regions of the virus. The study identified 6 out of 405 blood donors (1.5%) as positive for HTLV-1. Prevalence was higher among females (1.6%) compared to males (1.2%), with the age group of 46-64 years showing the highest positivity rate (4%). Phylogenetic analysis revealed that the LTR sequences of HTLV-1 in Jiroft were comparable to those circulating in Mashhad Province, with nine single-nucleotide polymorphisms (SNPs) identified in the LTR region of the isolates. The findings highlight the necessity for routine HTLV-1 screening among blood donors in Jiroft to ensure blood safety and mitigate the risk of transmission through transfusions. This study provides essential baseline data on HTLV-1 prevalence in Jiroft and contributes to the understanding of its genetic diversity, emphasizing the need for further research in this area.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting 2B-NSP coding region for colorimetric detection of foot-and-mouth disease virus in clinical samples. 针对2B-NSP编码区的泛血清型逆转录环介导等温扩增（RT-LAMP）法检测临床样品中口蹄疫病毒

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-26 DOI: 10.1007/s11262-025-02158-y

Jitendra K Biswal, Rajeev Ranjan, Jajati K Mohapatra, Nihar Ranjan Sahoo, Rabindra Prasad Singh

{"title":"Pan-serotype reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting 2B-NSP coding region for colorimetric detection of foot-and-mouth disease virus in clinical samples.","authors":"Jitendra K Biswal, Rajeev Ranjan, Jajati K Mohapatra, Nihar Ranjan Sahoo, Rabindra Prasad Singh","doi":"10.1007/s11262-025-02158-y","DOIUrl":"https://doi.org/10.1007/s11262-025-02158-y","url":null,"abstract":"Foot-and-mouth disease (FMD) is a highly contagious viral disease of even-toed animals. Rapid, early, and accurate diagnosis of the disease is important for the swift control of FMD. Although PCR-based assays are being used routinely for the effective diagnosis of FMD, these assays require sophisticated equipment, dedicated manpower, and complex procedures for the detection of amplified viral-genome. Colorimetric isothermal amplification assay with a sharp contrast in colour changes for the positive amplification of viral-genome would be qualified for quick and simple diagnosis of FMDV in both laboratory and field. Here, we report the development and evaluation of FMDV 2B-NSP coding region-based colorimetric RT-LAMP assay for pan-serotypic detection of viral-genome. Addition of 1 mg/ml of bovine serum albumin (BSA) as an additive, could reduce the detection time of the RT-LAMP assay from 60 to 30 min/reaction. Analytical sensitivity test showed that the RT-LAMP assay can detect up to 1000 copies of FMDV genome/reaction, simultaneously, the assay was found specific for the detection of FMDV genome as revealed on testing with serotypes O, A and Asia1 circulating in India during the last two decades. In addition, analysis of 312 clinical samples from various field outbreaks of FMDV in the country showed that RT-LAMP assay exhibited a sensitivity of 96.07%, and a specificity of 100% with an overall accuracy of 97.12%. Therefore, owing to the naked eye distinct visualization of amplified product (pink to yellow colour change), the RT-LAMP assay may facilitate rapid screening of FMD-suspected clinical samples without the use of hazardous DNA-binding dyes and simultaneously prevents aerosolization of amplified product, and subsequent carry over contamination in the diagnostic laboratory.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and phylogenetic analysis of influenza A and B viruses circulating in Sri Lanka following the COVID-19 pandemic. COVID-19大流行后在斯里兰卡流行的甲型和乙型流感病毒的分子和系统发育分析

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-25 DOI: 10.1007/s11262-025-02161-3

Thejanee Perera, Asanka Bowatte, Shanika Perera, Dinithi Rathnayaka, Vaithehi Francis, Shiyamalee Arunasalam, Sevwandi Abeywardana, Faseeha Noordeen, Saranga Sumathipala, Rohitha Muthugala

{"title":"Molecular and phylogenetic analysis of influenza A and B viruses circulating in Sri Lanka following the COVID-19 pandemic.","authors":"Thejanee Perera, Asanka Bowatte, Shanika Perera, Dinithi Rathnayaka, Vaithehi Francis, Shiyamalee Arunasalam, Sevwandi Abeywardana, Faseeha Noordeen, Saranga Sumathipala, Rohitha Muthugala","doi":"10.1007/s11262-025-02161-3","DOIUrl":"https://doi.org/10.1007/s11262-025-02161-3","url":null,"abstract":"Influenza viruses pose significant public health challenges, causing seasonal epidemics with high morbidity and mortality. This study sequenced influenza viral RNA from hospitalized patients with severe acute respiratory illness in Sri Lanka using an amplicon-based approach on the Illumina platform. Raw sequencing reads were quality checked using FASTP and Trimmomatic. Assembly was performed with SPAdes, and subtype identification was conducted using ABRIcate. Phylogenetic trees for HA and NA genes were generated in MEGA X and Geneious Prime and visualized with iTOL. Data analysis was performed using Galaxy and INSaFLU. Nineteen patient samples from different regions were successfully sequenced, identifying influenza A H1N1 (7/19), H3N2 (6/19), and influenza B (6/19). Notably, co-infection with H1N1 and the SARS-CoV-2 Omicron variant was observed, along with the co-circulation of influenza A H1N1, H3N2, and B strains in 2023. Molecular analysis revealed that all H1N1 and H3N2 strains carried mutations consistent with global strains. Influenza B strains also aligned with global trends. Key mutations affecting antigenicity and receptor binding were identified, highlighting viral evolution. This study explores the molecular evolution of influenza viruses in Sri Lanka (2021-2024) post-COVID-19. Findings underscore the need for continued molecular surveillance to inform public health strategies, particularly regarding co-infections and emerging mutations. However, this study did not assess the association between influenza genomic characteristics and disease severity; thus, future research could explore potential links between specific mutations, clades, or co-infections and clinical outcomes.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detectable episodic positive selection in the virion strand a-strain Maize streak virus genes may have a role in its host adaptation. 玉米条纹病毒链a株基因中可检测到的偶发性阳性选择可能在其宿主适应中起作用。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-16 DOI: 10.1007/s11262-025-02157-z

Kehinde A Oyeniran, Mobolaji O Tenibiaje

{"title":"Detectable episodic positive selection in the virion strand a-strain Maize streak virus genes may have a role in its host adaptation.","authors":"Kehinde A Oyeniran, Mobolaji O Tenibiaje","doi":"10.1007/s11262-025-02157-z","DOIUrl":"https://doi.org/10.1007/s11262-025-02157-z","url":null,"abstract":"Maize streak virus (MSV) has four genes: cp, encoding the coat protein; mp, the movement protein; and repA and rep, encoding two distinct replication-associated proteins from an alternatively spliced transcript. These genes play roles in encapsidation, movement, replication, and interactions with the external environment, making them prone to stimuli-driven molecular adaptation. We accomplished selection studies on publicly available curated, recombination-free, complete coding sequences for representative A-strain maize streak virus (MSV-A) cp and mp genes. We found evidence of gene-wide selection in these two MSV genes at specific sites within the genes (cp 1.23% and mp 0.99%). Positively selected sites have amino acids that are 60% hydrophilic and 40% hydrophobic in nature. We found significant evidence of positive selection at branches (cp: 0.76 and mp:1.66%) representing the diversity of MSV-A-strain in South Africa, which is related to the MSV-A-matA isolate (GenBank accession number: AF329881), well disseminated and adapted to the maize plant in sub-Saharan Africa. In the mp gene, selection significantly intensified for the overall diversities of the MSV-A sequences and those more related to the MSV-Mat-A isolate. These findings reveal that despite predominantly undergoing non-diversifying selection, the detectable diversifying positive selection observed in these genes may play a major role in MSV-A host adaptive evolution, ensuring sufficient pathogenicity for onward transmission without killing the host.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective inhibition of rabies virus gene expression by human miRNAs: a therapeutic approach using the 7mer-m8 model. 人mirna选择性抑制狂犬病毒基因表达：使用7mer-m8模型的治疗方法

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-15 DOI: 10.1007/s11262-025-02155-1

Aparajita Roy, Supriyo Chakraborty

{"title":"Selective inhibition of rabies virus gene expression by human miRNAs: a therapeutic approach using the 7mer-m8 model.","authors":"Aparajita Roy, Supriyo Chakraborty","doi":"10.1007/s11262-025-02155-1","DOIUrl":"https://doi.org/10.1007/s11262-025-02155-1","url":null,"abstract":"MicroRNAs, abbreviated as miRNAs, have a substantial impact on viral infections through their ability to control gene expression and influence the interactions between the host and the virus. This work investigates the capacity of miRNAs to selectively inhibit the expression of rabies virus genes, specifically Nucleoprotein N, Phosphoprotein M1 and M2, Transmembrane Glycoprotein G, and L protein. The 7mer-m8 model was utilized to identify human miRNAs that target these viral genes. The interactions between the miRNAs and the genes were then assessed based on binding energy, GC content, and stability. The findings indicated that miRNAs, including miR-1279, miR-4251, miR-4288, and miR-12117, successfully target the N gene. In addition, other miRNAs target the remaining viral genes, indicating their capacity to bind and potentially inhibit viral replication. In addition, docking experiments have verified the stability of miRNA-mRNA duplexes, as evidenced by the high free energy values that indicate strong and reliable contacts between miRNA and gene. These findings indicate that certain human miRNAs have the potential to be effective therapeutic agents against the rabies virus by suppressing gene expression. This offers a new and innovative strategy to fight against this deadly infection.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0