Virus Genes最新文献_第3页

Long non-coding RNA C1RL-AS1 aggravates influenza A virus pneumonia through miR-16-5p/LAMP3. 长链非编码RNA C1RL-AS1通过miR-16-5p/LAMP3加重甲型流感病毒肺炎

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-01-02 DOI: 10.1007/s11262-024-02131-1

Xingjuan Liao, Qin Liang, Chao Xu, Xinbing Luo

{"title":"Long non-coding RNA C1RL-AS1 aggravates influenza A virus pneumonia through miR-16-5p/LAMP3.","authors":"Xingjuan Liao, Qin Liang, Chao Xu, Xinbing Luo","doi":"10.1007/s11262-024-02131-1","DOIUrl":"10.1007/s11262-024-02131-1","url":null,"abstract":"Influenza A viruses continue to pose a serious threat to public health and economic stability. To investigate the role of C1RL-AS1 in influenza A virus (IAV) pneumonia. Using RT-qPCR analysis, we determined C1RL-AS1 expression levels in children with IAV-infected pneumonia and A549 cells. C1RL-AS1 expression levels in children were subjected to ROC analysis. C1RL-AS1 was knocked down to investigate its role in IAV-infected A549 cells, including effects on viral nucleoprotein (NP) production, cell survival, and apoptosis. Downstream miRNAs of C1RL-AS1 were predicted and validated. MiR-16-5p target genes were predicted and validated. C1RL-AS1 was up-regulated in IAV-infected children and A549 cells. C1RL-AS1 expression levels distinguished children with IAV pneumonia from healthy children. Knockdown of C1RL-AS1 attenuated viral NP production, promoted A549 cell survival, and inhibited apoptosis. MiR-16-5p was a downstream C1RL-AS1 miRNA. miR-16-5p counteracted the anti-IAV infection effect brought about by C1RL-AS1 knockdown. LAMP3 was a miR-16-5p target gene associated with pneumonia. LAMP3 restored the cellular effects brought about by C1RL-AS1/miR-16-5p co-knockdown. C1RL-AS1 is a possible diagnostic factor for IAV pneumonia in children. C1RL-AS1 may participate in IAV pneumonia by sponging miR-16-5p and then moderating LAMP3.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"145-152"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First detection and diversity of astroviruses in wild migratory birds of Sakhalin Island, North Pacific. 北太平洋库页岛野生候鸟中星状病毒的首次检测及其多样性。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-27 DOI: 10.1007/s11262-024-02130-2

Dmitry Zhirov, Nikita Dubovitskiy, Anastasiya Derko, Arina Loginova, Ivan Sobolev, Pavel Ktitorov, Olga Kulikova, Guimei He, Zhenghuan Wang, Wen Wang, Alexander Alekseev, Alexander Shestopalov, Kirill Sharshov

{"title":"First detection and diversity of astroviruses in wild migratory birds of Sakhalin Island, North Pacific.","authors":"Dmitry Zhirov, Nikita Dubovitskiy, Anastasiya Derko, Arina Loginova, Ivan Sobolev, Pavel Ktitorov, Olga Kulikova, Guimei He, Zhenghuan Wang, Wen Wang, Alexander Alekseev, Alexander Shestopalov, Kirill Sharshov","doi":"10.1007/s11262-024-02130-2","DOIUrl":"10.1007/s11262-024-02130-2","url":null,"abstract":"Researchers have identified Avastrovirus as a significant genus of bird viruses, linked to various avian diseases such as enteritis, growth retardation, nephritis and hepatitis. These infections can cause substantial economic losses in agrocultureand have a widespread impact on global food production. Although there have been numerous studies on these viruses, most of them-mainly focuses on poultry. Research on astroviruses in wild bird populations has revealed a wide genetic diversity of these viruses, yet our understanding of their biological and ecological characteristics remains limited. In this study, we for the first time detected avastrovirus in wild migratory birds of the families Anatidae and Columbidae from Sakhalin Island, North Pacific Ocean. Phylogenetic analysis revealed the presence of Avastrovirus 2 in wild doves and Avastrovirus 3 in wild ducks. These findings provide valuable insights into the circulation of astroviruses in wild bird populations of Sakhalin Island, which lies along the East Asian-Australasian Flyway.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"244-248"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete genomic sequence of Magnaporthe oryzae polymycovirus 1. 米大孔菌多分枝病毒1型全基因组序列。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-06 DOI: 10.1007/s11262-024-02126-y

Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng

{"title":"The complete genomic sequence of Magnaporthe oryzae polymycovirus 1.","authors":"Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng","doi":"10.1007/s11262-024-02126-y","DOIUrl":"10.1007/s11262-024-02126-y","url":null,"abstract":"A novel double-stranded RNA virus, designated as \"Magnaporthe oryzae polymycovirus 1\" (MoPmV1), was identified in Magnaporthe oryzae strain TM02. MoPmV1 has four dsRNA fragments, ranging from 1324 to 2401 bp in length. DsRNA1, 2, and 3 of MoPmV1 each possess a single large open reading frame (ORF), whereas dsRNA4 contains two ORFs. BLASTp analysis indicated that ORF1a, encoded by dsRNA1, shows 59.2% amino acid sequence identity with the RNA-dependent RNA polymerase (RdRp) of Beauveria bassiana polymycovirus 2; ORF2a, encoded by dsRNA2, shows 42.3% identity with the putative serine protease of Phaeoacremonium minimum tetramycovirus 1; ORF3a, encoded by dsRNA3, shows 40.6% identity with the methyltransferase of Aspergillus fumigatus tetramycovirus 1; ORF4a, encoded by dsRNA4, shows 41.7% identity with the proline-alanine-serine-rich (PASr) protein of Botryosphaeria dothidea virus 1, while ORF4b, encoded by dsRNA4, shows no significant similarity to any known proteins. Phylogenetic analysis of the RdRp domain indicated that MoPmV1 was grouped in a cluster with members of the genus Polymycovirus in the family Polymycoviridae. Based on these characteristics, MoPmV1 is a new member of the genus Polymycovirus in the family Polymycoviridae. This is the first report of a mycovirus of the family Polymycoviridae identified in rice blast fungus M. oryzae.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"204-210"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of thymidine kinase-deficiency (∆ORF38) on neuropathogenicity of equine herpesvirus-1 in the mouse model and expression of neighboring genes. 胸苷激酶缺乏（∆ORF38）对马疱疹病毒-1小鼠模型神经致病性及邻近基因表达的影响

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-16 DOI: 10.1007/s11262-024-02128-w

Samy Kasem, Ahmed S Abdel-Moneim, Hideto Fukushi

引用次数: 0

Pooled screening for endogenous HHV-6 in subjects with coronary artery disease. 冠状动脉疾病患者内源性HHV-6的合并筛查

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-01-31 DOI: 10.1007/s11262-025-02134-6

Rie Koide, Yoshie Nakashima, Shohei Kojima, Yohei Sotomi, Yasushi Sakata, Yasuhiko Sakata, Kaoru Ito, Nicholas F Parrish

引用次数: 0

ALR inhibits HBV replication and autophagosome formation by ameliorating HBV-induced ROS production in hepatic cells. ALR通过改善肝细胞中HBV诱导的ROS产生来抑制HBV复制和自噬体的形成。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-02-11 DOI: 10.1007/s11262-025-02139-1

Amit Kumar Mishra, Md Musa Hossain, Teja Naveen Sata, Kishor Pant, Ajay K Yadav, Amrendra Kumar Sah, Parul Gupta, Md Ismail, Baibaswata Nayak, Shalimar, Senthil Kumar Venugopal

{"title":"ALR inhibits HBV replication and autophagosome formation by ameliorating HBV-induced ROS production in hepatic cells.","authors":"Amit Kumar Mishra, Md Musa Hossain, Teja Naveen Sata, Kishor Pant, Ajay K Yadav, Amrendra Kumar Sah, Parul Gupta, Md Ismail, Baibaswata Nayak, Shalimar, Senthil Kumar Venugopal","doi":"10.1007/s11262-025-02139-1","DOIUrl":"10.1007/s11262-025-02139-1","url":null,"abstract":"HBV has a small genome and thrives in the infected hepatocytes by hijacking the cellular machinery and cellular pathways. HBV induces incomplete autophagy for its replication and survival. This study showed that HBV replication induces Reactive oxygen species (ROS) production, which in turn augments the formation of autophagosomes. Augmenter of liver regeneration (ALR) is a sufhydryl oxidase and has an anti-oxidative property. We sought to determine the interplay between HBV and antioxidant protein ALR. We showed that HBV downregulated ALR expression in hepatic cells. There was increased ROS production in HBV-infected cells while ALR downregulated ROS levels and expression of NADPH oxidase NOX4. N-acetyl cysteine, a ROS scavenger, downregulated ROS level and autophagosome formation in HBV-expressing cells. ALR overexpression in HBV-expressing cells downregulated the expression of autophagy marker proteins while upregulated the expression of p-MTOR. ALR overexpression decreased the expression of HBx, HBsAg, and total HBV load. This study showed that HBx relieved ALR-mediated inhibition by upregulating the miR-181a expression in HBV-infected cells, which in turn downregulated ALR expression.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"167-178"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral siRNA delivered using attenuated, anthrax toxin protects cells from the cytopathic effects of Zika virus. 使用减毒炭疽毒素递送的抗病毒siRNA可保护细胞免受寨卡病毒的细胞病变影响。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-03-30 DOI: 10.1007/s11262-025-02152-4

Benedita K L Feron, Timothy Gomez, Natalie C Youens, Nourhan A M Mahmoud, Hadeer K S Abdelrahman, Joachim J Bugert, Simon C W Richardson

{"title":"Antiviral siRNA delivered using attenuated, anthrax toxin protects cells from the cytopathic effects of Zika virus.","authors":"Benedita K L Feron, Timothy Gomez, Natalie C Youens, Nourhan A M Mahmoud, Hadeer K S Abdelrahman, Joachim J Bugert, Simon C W Richardson","doi":"10.1007/s11262-025-02152-4","DOIUrl":"https://doi.org/10.1007/s11262-025-02152-4","url":null,"abstract":"Curative drugs are needed for the treatment of viral infections. Small interfering (si)RNA offer such a prospect but require the development of safe, effective and non-hepatotropic subcellular delivery systems. Here, 5 candidate siRNA molecules targeting defined sequences within the Zika Virus (ZIKV) genome were assayed for their ability to reduce ZIKV induced cytopathic effects in vitro. The protection of Huh-7 cells from ZIKV cytopathic effects was recorded after electroporation and the siRNA Feron-Zv2, resulting in 122.7 ± 5.3% cell viability (n = 3 ± standard error of the mean (SEM), 100 nM siRNA) after exposure to ZIKV relative to a virus treated control (35.2 ± 7.1% cell viability (n = 3 ± SEM)). Protection of BHK-21 cells was recorded after transfection with an attenuated anthrax toxin containing an RNA binding domain. Treatment with Feron-Zv4 resulted in 75.1 ± 2.9% cell viability (n = 3 ± SEM, 25 nM siRNA) after exposure to ZIKV. This protection was mirrored by a system containing octameric PA where a maximum of 86.2 ± 4.4% cell viability was reported (n = 3 ± SEM, 75 nM siRNA) after treatment with Feron-Zv2. Scrambled siRNA afforded no measurable protection. Here we report for the first time that siRNA delivered by either attenuated anthrax toxin or octamer forming ATx can protect mammalian cells from ZIKV cytopathic effects.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and drug resistance pattern in pol gene of HIV-1 sub-subtypes circulating in Lahore, Pakistan. 巴基斯坦拉合尔流行HIV-1亚型pol基因的分子特征和耐药模式

IF 1.9 4区医学

Virus Genes Pub Date : 2025-03-27 DOI: 10.1007/s11262-025-02149-z

Akmal Zubair, Adnan Zeb, Syed Ahsan Shahid, Hasnain Javed, Muhammad Ali

{"title":"Molecular characterization and drug resistance pattern in pol gene of HIV-1 sub-subtypes circulating in Lahore, Pakistan.","authors":"Akmal Zubair, Adnan Zeb, Syed Ahsan Shahid, Hasnain Javed, Muhammad Ali","doi":"10.1007/s11262-025-02149-z","DOIUrl":"https://doi.org/10.1007/s11262-025-02149-z","url":null,"abstract":"This study aimed to determine sub-subtypes and circulating recombinant forms (CRFs) in Pakistan using the pol gene, identification of amino acid substitutions, structural changes, and drug resistance evaluation in the p66 region of reverse transcriptase. A total of 50 HIV-positive blood samples were collected from Lahore Pakistan, confirmed by Real Time-Polymerase Chain Reaction. The samples were further processed for pol gene amplification followed by nucleotide sequencing through the Sanger method. Out of 50 samples, 26 samples were amplified, and 14 sequences were obtained. The sequences were aligned with reference sequences to determine subtyping and phylogenetic analysis. Moreover, amino acid substitutions and drug resistance patterns were also determined in the RTp66 region. Phylogenetic analysis showed that 8 sequences of our isolates were closely related to circulating recombinant form (CRF43_02 G), and 3 sequences were of CRF30_026 (CRF02_AG) subtypes while the remaining 3 sequences were related to CRF35_A1D, CRF95_02B (CRF02_AG) and Subtype G of HIV-1. Several amino acid substitutions were identified in our isolates which show no impact on the structure of protein. Furthermore, the isolate QAU-AZ2 (OR086936) proposes variable degree of resistance to nevirapine (NVP), etravirine (ETR), rilpivirine (RPV), efavirenz (EFV), Doravirine (DOR); while no resistance against NNTRI and NTRI was observed in the remaining isolates. Further studies are required to (i) examine the function of identified amino acid substitutions through molecular docking, and (ii) sequence the complete pol gene of the viral isolates from Pakistani patients to determine possible drug resistance associated with amino acid substitutions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143722006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Complete genome and molecular characterization of spiranthes mosaic virus 3 from Phlox paniculata. 更正：夹竹桃螺旋体花叶病毒3的全基因组和分子特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-03-26 DOI: 10.1007/s11262-025-02153-3

Elena Motsar, Fedor Sharko, Anna Sheveleva, Larisa Savostyanova, Irina Mitrofanova, Sergei Chirkov

引用次数: 0

Alphanudiviral segments found in transcriptomes of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). 在荨麻疹叶螨（螨亚纲：叶螨科）双斑蜘蛛螨转录组中发现的甲病毒片段。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-03-21 DOI: 10.1007/s11262-025-02150-6

Ethiane Rozo Santos, Jirka Manuel Petersen, Thaís Danielle Duarte Santana, Robert L Harrison, Daniel M P Ardisson-Araújo

{"title":"Alphanudiviral segments found in transcriptomes of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae).","authors":"Ethiane Rozo Santos, Jirka Manuel Petersen, Thaís Danielle Duarte Santana, Robert L Harrison, Daniel M P Ardisson-Araújo","doi":"10.1007/s11262-025-02150-6","DOIUrl":"https://doi.org/10.1007/s11262-025-02150-6","url":null,"abstract":"Nudiviruses (family Nudiviridae) are a diverse group of enveloped, rod-shaped viruses with double-stranded DNA genomes that infect a wide range of insects and crustaceans. These viruses are of significance both as biological control agents in agriculture and as agents of disease in aquaculture and insect rearing. In this work, we found four segments of a novel and divergent nudivirus identified through RNA-seq data from the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). The sequences of this virus were detected only in a subset of mite transcriptomes. The assembled segments covered a total of 100,780 bp, with 122 annotated ORFs, including all the 28 conserved nudiviral core genes. Phylogenetic analysis based on the predicted amino acid sequences of 17 selected nudiviral core genes placed the virus within the Alphanudivirus genus, in a clade containing nudiviruses identified from flea transcriptomes. This placement was confirmed by phylogenies of segment-specific concatenated core gene alignments. Indeed, the virus was designated as Tetranychus urticae alphanudivirus (TuNV). Transcriptional profiling revealed variable levels of transcriptional activity among genomic segments and viral genes. Arthropod gene homologs were found interspersed among nudiviral genes across all segments along with several unique genes. This genomic and phylogenetic characterization enhances our understanding of nudivirus diversity and evolution within arthropod hosts.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0