Virus Genes最新文献_第3页

A new isolate of mungbean yellow mosaic India virus in Vigna mungo L. reported from a Dayalbagh field, Agra. 从阿格拉的 Dayalbagh 农田中报告了绿豆黄镶嵌印度病毒的新分离株。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-21 DOI: 10.1007/s11262-024-02099-y

Ambika Chaturvedi, Dipinte Gupta, Bikash Mandal, Rajiv Ranjan

{"title":"A new isolate of mungbean yellow mosaic India virus in Vigna mungo L. reported from a Dayalbagh field, Agra.","authors":"Ambika Chaturvedi, Dipinte Gupta, Bikash Mandal, Rajiv Ranjan","doi":"10.1007/s11262-024-02099-y","DOIUrl":"10.1007/s11262-024-02099-y","url":null,"abstract":"Black gram (Vigna mungo L.) plants showing yellow mosaic symptoms during 2019-2022 crop seasons were collected randomly from a Dayalbagh field, Agra Region of Uttar Pradesh, India. Total genomic DNA was isolated from the infected leaf samples by the Cetyltrimethylammonium bromide (CTAB) method and subjected to PCR. After viral confirmation, the viral genome was amplified by rolling circle amplification following the standard protocol. The DNA A and DNA B subgenomes were cloned individually as a PstI and BamHI fragment in the pUC18 vector. Positive clones were subjected to DNA sequencing. The results revealed that DNA A and DNA B show the closest nucleotide identity with \"mungbean yellow mosaic India virus-[Mungbean], DNA-A, the complete sequence\" (GeneBank Accession No AF416742.1) with 98.14% identity, and \"mungbean yellow mosaic India virus isolate Mu1-Dholi segment DNA-B, the complete sequence\" (GeneBank Accession No MW814723.1) with 97.94% identity, respectively. The new isolate of mungbean yellow mosaic India virus (MYMIV) shows sequence similarity with the coat protein gene of various strains of MYMIV. In the new isolate of MYMIV, a point mutation was observed at the 2036th nucleotide of DNA B, which disrupts the reading frame to introduce a stop codon and thus leading to a decrease in the size of the movement protein gene. In the present study we are reporting the whole genome sequence of the MYMIV Dayalbagh isolate for the first time.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"747-751"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of mycoviruses in transcriptomes from the fungal family ceratocystidaceae. 鉴定和表征陶瓷囊菌科真菌转录组中的霉菌病毒。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-10-08 DOI: 10.1007/s11262-024-02112-4

Bianca Hough, Brenda Wingfield, David Read

{"title":"Identification and characterization of mycoviruses in transcriptomes from the fungal family ceratocystidaceae.","authors":"Bianca Hough, Brenda Wingfield, David Read","doi":"10.1007/s11262-024-02112-4","DOIUrl":"10.1007/s11262-024-02112-4","url":null,"abstract":"Mycoviruses pervade the fungal kingdom, yet their diversity within various fungal families and genera remains largely unexplored. In this study, 10 publicly available fungal transcriptomes from Ceratocystidaceae were analyzed for the presence of mycoviruses. Despite mycovirus associations being known in only four members of this family, our investigation unveiled the discovery of six novel mycoviruses. The majority of these mycoviruses are composed of positive sense single stranded RNA and are putatively assigned to the viral family Mitoviridae (with tentative classification into the genera Unuamitovirus and Duamitovirus). The double stranded RNA viruses, however, were associated with the family Totiviridae (with tentative classification into the genus Victorivirus). This study also revealed the discovery of an identical unuamitovirus in the fungal species Thielaviopsis ethacetica and Thielaviopsis paradoxa. This discovery was notable as these fungal isolates originated from distinct geographical locations, highlighting potential implications for the transmission of this mitovirus. Moreover, this investigation significantly expands the known host range for mycoviruses in this family, marking the initial identification of mycoviruses within Ceratocystis platani, Thielaviopsis paradoxa, Thielaviopsis ethacetica, and Huntiella omanensis. Future research should focus on determining the effects that these mycoviruses might have on their fungal hosts.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"696-710"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines. 利用 YBX3 基因敲除和过表达细胞系，探索 YBX3 在 PEDV 感染中的作用。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s11262-024-02109-z

Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang

{"title":"Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.","authors":"Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang","doi":"10.1007/s11262-024-02109-z","DOIUrl":"10.1007/s11262-024-02109-z","url":null,"abstract":"Porcine epidemic diarrhea (PED) is a highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV), which results in significant economic losses. PEDV infection causes severe damage to the midgut barrier in the small intestine. YBX3, an important protein in tight junctions, promotes epithelial cell proliferation. However, its role in the process of PEDV infection remains unclear. In this study, we observed a significant increase in mRNA expression of YBX3 following PEDV infection. Additionally, the protein expression of YBX3 showed an initial increase followed by a decrease over time. Furthermore, treatment with 2% DSS resulted in a significant down-regulation of YBX3 mRNA and protein expression. Furthermore, we successfully generated knockout and overexpression cell lines of YBX3. Preliminary assays indicated that elevated expression of YBX3 inhibited the PEDV replication, while knockout of YBX3 had the opposite effect. In conclusion, our study has preliminarily revealed the functional role of YBX3 during PEDV infection. This finding lays the foundation for further investigation into its mechanism in future and also provides new insights into the mechanism of PEDV-host interactions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"667-673"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of the novel phage SAP71 and its potential use against Staphylococcus aureus in an atopic dermatitis mouse model. 新型噬菌体 SAP71 的分离及其在特应性皮炎小鼠模型中对抗金黄色葡萄球菌的潜力。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s11262-024-02106-2

Huaixin Geng, Xin Yang, Chenghui Zou, Wen Zhang, Jingheng Xiang, Kailang Yang, Yi Shu, Guangxin Luan, Xu Jia, Mao Lu

引用次数: 0

Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen. 表达orf病毒的疫苗病毒H3L跨膜蛋白类似物F1L，并将其成功纯化为诊断抗原。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-13 DOI: 10.1007/s11262-024-02097-0

Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal

{"title":"Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen.","authors":"Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal","doi":"10.1007/s11262-024-02097-0","DOIUrl":"10.1007/s11262-024-02097-0","url":null,"abstract":"Orf or contagious ecthyma is a highly contagious, zoonotic, and economically important global viral disease of small ruminants and is endemic in India. Vaccination of susceptible goats/sheep along with suitable recombinant protein-based serological assay will be useful in the control of the infection. In this study, the full-length and truncated versions of F1L encoding gene (ORF 059) of orf virus were cloned into pFasBac HT A vector, transformed in DH10Bac cells, and expressed in insect cells. The full-length and truncated recombinant F1L proteins were expressed as a 6 × histidine-tagged fusion protein for ease of purification by Ni-NTA affinity chromatography under denaturing conditions. A protein with ~ 40 kDa and ~ 35 kDa for full-length and truncated F1L protein, respectively, were expressed and confirmed by SDS-PAGE and western blot. The protein reactivity evaluated by western blot analysis and indirect ELISA using ORFV hyperimmune serum was also found to be reactive. The results of the present study showed that the purified recombinant F1L protein can be used as a diagnostic antigen in sero-surveillance of ORFV infection in small ruminants. To the best of authors' knowledge, this is the first report on the expression of ORFV F1L in insect cells using a baculovirus vector and its successful purification to use as the potential diagnostic antigen in ELISA.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"642-651"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of Klebsiella phages with lytic activity against Klebsiella pneumoniae. 对肺炎克雷伯氏菌具有溶菌活性的克雷伯氏菌噬菌体的特征和基因组分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-11-15 DOI: 10.1007/s11262-024-02123-1

Shanzheng Bi, Hong Peng, Xiao Wei, Changjun Wang, Xiangna Zhao

引用次数: 0

Establishment and application of a duplex fluorescent quantitative PCR assay for H9N2 subtype avian influenza virus and infectious bronchitis virus. 建立和应用 H9N2 亚型禽流感病毒和传染性支气管炎病毒的双重荧光定量 PCR 检测方法。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-11-11 DOI: 10.1007/s11262-024-02121-3

Wanting Zhou, Qiuyan Mao, Shuning Zhou, Tingting Li, Jie Tian, Xiaoqi Li, Shuo Liu, Cheng Peng, Zhibin Hu, Jinping Li, Guangyu Hou, Houhui Song, Wenming Jiang, Hualei Liu

{"title":"Establishment and application of a duplex fluorescent quantitative PCR assay for H9N2 subtype avian influenza virus and infectious bronchitis virus.","authors":"Wanting Zhou, Qiuyan Mao, Shuning Zhou, Tingting Li, Jie Tian, Xiaoqi Li, Shuo Liu, Cheng Peng, Zhibin Hu, Jinping Li, Guangyu Hou, Houhui Song, Wenming Jiang, Hualei Liu","doi":"10.1007/s11262-024-02121-3","DOIUrl":"https://doi.org/10.1007/s11262-024-02121-3","url":null,"abstract":"The H9N2 subtype of avian influenza virus (AIV) and infectious bronchitis virus (IBV) are important avian viruses that cause respiratory symptoms in poultry, and can form mixed infections. In this study, primers and probes were designed based on the HA gene of H9N2 and the 5' noncoding region of IBV, respectively, and a fluorescent quantitative RT-PCR assay was established for simultaneous detection of these two pathogens. The reaction system and conditions were optimized. The method only detected AIV subtype H9N2 and IBV and no other viruses, confirming its high specificity. The assay detected 13.5 copies/μL and 1.66 copies/μL of H9N2 and IBV in clinical samples, respectively. The coefficients of variation for intra- and interassay repeatability were < 3%. The established method was used to analyze 254 clinical samples (oropharyngeal and cloacal swabs) from Hubei Province, China; 98.82% were positive for both pathogens. In summary, a duplex fluorescent quantitative RT-PCR method capable of simultaneously detecting AIV subtype H9N2 and IBV was established. It is specific, sensitive, and reproducible, and can be used for diagnosis of a variety of clinical samples. It provides a technological means for the rapid and simultaneous detection of both pathogens, and thus can facilitate clinical diagnosis and epidemiological investigations.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142636191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization, phylogeny and recombination of Rhynchosia yellow mosaic virus infecting Rhynchosia minima, a wild relative of pigeonpea (Cajanus cajan) from India. 感染印度鸽子豆（Cajanus cajan）野生近缘植物 Rhynchosia minima 的 Rhynchosia 黄镶嵌病毒的特征、系统发育和重组。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-11-06 DOI: 10.1007/s11262-024-02120-4

Mohammad Akram, Naimuddin Kamaal, Deepender Kumar, Dibendu Datta, Aniruddha Kumar Agnihotri

{"title":"Characterization, phylogeny and recombination of Rhynchosia yellow mosaic virus infecting Rhynchosia minima, a wild relative of pigeonpea (Cajanus cajan) from India.","authors":"Mohammad Akram, Naimuddin Kamaal, Deepender Kumar, Dibendu Datta, Aniruddha Kumar Agnihotri","doi":"10.1007/s11262-024-02120-4","DOIUrl":"https://doi.org/10.1007/s11262-024-02120-4","url":null,"abstract":"Rhynchosia minima grown at Indian Institute of Pulses Research, Kanpur, India, showed yellow mosaic symptoms on leaves and were suspected to be caused by begomovirus(es). Leaves from five different plants (Rhm1-Rhm5) were tested for the presence of four viruses in PCR. PCR assays revealed the presence of mungbean yellow mosaic India virus in four samples, whereas one sample (Rhm2) was negative. Processing of Rhm2 sample using rolling circle amplification and restriction digestion indicated the presence of DNA molecules of ~ 2.6-2.7 kb. These molecules were sequenced after cloning and found to be of 2741 and 2658 nucleotides in size. BLAST analysis revealed that DNA-A (OQ269467) and DNA-B (OQ269468) molecules of rhynchosia yellow mosaic virus (RhYMV) with 99.09% and 93.74% nucleotide similarity with DNA-A (KP752090) and DNA-B (KP752091) of the RhYMV isolate, respectively. These sequences had a genome organization typical of legume-infecting Old World bipartite begomoviruses. Full genome sequences obtained from Rhm2 are, therefore, considered to be an isolate of RhYMV, designated as RhYMV-IN-Knp. The phylogenetic analysis revealed that RhYMV-IN-Knp was grouped with other isolates of RhYMV followed by Cajanus scarabaeoides yellow mosaic virus. DNA-A of RhYMV-IN-Knp showed two recombination events. The Old World bipartite begomovirus squash leaf curl China virus (AM260205) was identified as the major parent, whereas New World bipartite begomovirus rhynchosia golden yellow mosaic Yucatan virus (EU021216) was identified as the minor parent. RhYMV holds the potential of infecting cultivated legume crops, therefore regular monitoring is crucial especially for pigeonpea breeding programs.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of influenza B virus and oseltamivir resistance in pediatric patients with acute respiratory infections: a cross-sectional study. 急性呼吸道感染儿科患者的乙型流感病毒基因特征和奥司他韦耐药性：一项横断面研究。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-11-05 DOI: 10.1007/s11262-024-02119-x

Sheida Alizadeh, Fahime Edalat, Arash Letafati, Neda Pirbonyeh, Alireza Tabibzadeh, Leila Mousavizadeh, Afagh Moattari, Mohammad Hadi Karbalaie Niya

{"title":"Genetic characterization of influenza B virus and oseltamivir resistance in pediatric patients with acute respiratory infections: a cross-sectional study.","authors":"Sheida Alizadeh, Fahime Edalat, Arash Letafati, Neda Pirbonyeh, Alireza Tabibzadeh, Leila Mousavizadeh, Afagh Moattari, Mohammad Hadi Karbalaie Niya","doi":"10.1007/s11262-024-02119-x","DOIUrl":"https://doi.org/10.1007/s11262-024-02119-x","url":null,"abstract":"Influenza virus neuraminidase inhibitors (NAIs) drug usage can result in NAI resistance, especially in children and individuals with weakened immune systems. The aim of the present study was to identify NAI-resistant variants of IBV and to introduce probable novel mutations, phylogenetic study, and its epitope mapping based on NA gene in patients from Shiraz, Iran. A cross-sectional study was conducted between 2017 and 2018 on symptomatic children. A real-time PCR was run for IBV screening. Then, making use of direct sequencing, amplified 1401 bases of NA gene and phylogenetic tree reconstructed. Epitopes were predicted using ABCpred server. From among a total of 235 specimens, 9.7% were identified with IBV infection. Of them, sequence of NA gene for 17 isolates were analyzed. Phylogenetic analysis showed that 15 isolates belonged to Yamagata clade 3 Wisconsin/01-like subclade and 2 were related to Victoria clade 1 Brisbane/60-like subclade (Vic-1A-2). NA gene sequence analysis showed a total of 52 substitutions in which 27 were for BVic and 37 were for BYam isolates and 19 were novel substitutions. Only one substitution (S198N) was found in NA active site and T49M, I120V, N198S, N219K, S295R, D320K N340D, E358K, D384G, and D463N were found as probable resistance variants to NAIs. Epitope mapping showed some major differences in our isolates NA gene. Present study was one of the rare comprehensive studies conducted in Shiraz/Iran on IBV resistant associated variants to NAIs. We reported 11.7% mutation in NA active site and some probable NAIs resistant mutations. Epitope mapping confirmed major changes in NA gene which needs broader studies to confirm.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lumpy skin disease: a systematic review of mode of transmission, risk of emergence, and risk entry pathways. 结节性皮肤病：传播方式、出现风险和风险进入途径的系统回顾。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-22 DOI: 10.1007/s11262-024-02117-z

Bhawanpreet Kaur, Sehajpal Singh Dhillon, Amarpreet Singh Pannu, C S Mukhopadhyay

引用次数: 0