Virus Genes最新文献_第3页

Lumpy skin disease: a systematic review of mode of transmission, risk of emergence, and risk entry pathways. 结节性皮肤病：传播方式、出现风险和风险进入途径的系统回顾。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-22 DOI: 10.1007/s11262-024-02117-z

Bhawanpreet Kaur, Sehajpal Singh Dhillon, Amarpreet Singh Pannu, C S Mukhopadhyay

引用次数: 0

Genetic characterization of influenza B virus and oseltamivir resistance in pediatric patients with acute respiratory infections: a cross-sectional study. 急性呼吸道感染儿科患者的乙型流感病毒基因特征和奥司他韦耐药性：一项横断面研究。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-11-05 DOI: 10.1007/s11262-024-02119-x

Sheida Alizadeh, Fahime Edalat, Arash Letafati, Neda Pirbonyeh, Alireza Tabibzadeh, Leila Mousavizadeh, Afagh Moattari, Mohammad Hadi Karbalaie Niya

{"title":"Genetic characterization of influenza B virus and oseltamivir resistance in pediatric patients with acute respiratory infections: a cross-sectional study.","authors":"Sheida Alizadeh, Fahime Edalat, Arash Letafati, Neda Pirbonyeh, Alireza Tabibzadeh, Leila Mousavizadeh, Afagh Moattari, Mohammad Hadi Karbalaie Niya","doi":"10.1007/s11262-024-02119-x","DOIUrl":"10.1007/s11262-024-02119-x","url":null,"abstract":"Influenza virus neuraminidase inhibitors (NAIs) drug usage can result in NAI resistance, especially in children and individuals with weakened immune systems. The aim of the present study was to identify NAI-resistant variants of IBV and to introduce probable novel mutations, phylogenetic study, and its epitope mapping based on NA gene in patients from Shiraz, Iran. A cross-sectional study was conducted between 2017 and 2018 on symptomatic children. A real-time PCR was run for IBV screening. Then, making use of direct sequencing, amplified 1401 bases of NA gene and phylogenetic tree reconstructed. Epitopes were predicted using ABCpred server. From among a total of 235 specimens, 9.7% were identified with IBV infection. Of them, sequence of NA gene for 17 isolates were analyzed. Phylogenetic analysis showed that 15 isolates belonged to Yamagata clade 3 Wisconsin/01-like subclade and 2 were related to Victoria clade 1 Brisbane/60-like subclade (Vic-1A-2). NA gene sequence analysis showed a total of 52 substitutions in which 27 were for BVic and 37 were for BYam isolates and 19 were novel substitutions. Only one substitution (S198N) was found in NA active site and T49M, I120V, N198S, N219K, S295R, D320K N340D, E358K, D384G, and D463N were found as probable resistance variants to NAIs. Epitope mapping showed some major differences in our isolates NA gene. Present study was one of the rare comprehensive studies conducted in Shiraz/Iran on IBV resistant associated variants to NAIs. We reported 11.7% mutation in NA active site and some probable NAIs resistant mutations. Epitope mapping confirmed major changes in NA gene which needs broader studies to confirm.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"54-63"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Domestic cat hepadnavirus genotype B is present in Southern Brazil. 巴西南部存在家猫狂犬病毒基因 B 型。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-14 DOI: 10.1007/s11262-024-02115-1

Alaíse Tessmann, Juliana Sumienski, Alexandre Sita, Larissa Mallmann, Gabriela Espíndola Birlem, Nilson Júnior da Silva Nunes, Camila Gottlieb Lupion, Juliana Schaeffer Eckert, Meriane Demoliner, Juliana Schons Gularte, Paula Rodrigues de Almeida, Fernando Rosado Spilki, Matheus Nunes Weber

{"title":"Domestic cat hepadnavirus genotype B is present in Southern Brazil.","authors":"Alaíse Tessmann, Juliana Sumienski, Alexandre Sita, Larissa Mallmann, Gabriela Espíndola Birlem, Nilson Júnior da Silva Nunes, Camila Gottlieb Lupion, Juliana Schaeffer Eckert, Meriane Demoliner, Juliana Schons Gularte, Paula Rodrigues de Almeida, Fernando Rosado Spilki, Matheus Nunes Weber","doi":"10.1007/s11262-024-02115-1","DOIUrl":"10.1007/s11262-024-02115-1","url":null,"abstract":"Domestic cat hepadnavirus (DCH) (Orthohepadnavirus felisdomestici) is an emerging virus related to the hepatitis B virus (HBV) already reported in many countries. The molecular prevalence of DCH varies widely in the regions investigated so far. In the present work, we reported the presence of DCH in Brazil. Sixty cat serum samples tested by DCH presence using PCR and 1.67% (1/60) were positive, similar to the low positive molecular rates reported in United States and Japan. The DCH full-length genome was classified in genotype B, which is uncommon since this genotype was only reported once in Japan. The DCH-positive sample was obtained in a stray cat female apparently healthy, presenting ALT, AST, and ALKP normal values, and negative for FIV and FeLV. Due the low positivity rate detected, some factors as alteration in hepatic enzymes and FIV/FeLV infection could not be evaluated. Other works are necessary to statistically validate these observations in Brazil.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"81-86"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of Klebsiella phages with lytic activity against Klebsiella pneumoniae. 对肺炎克雷伯氏菌具有溶菌活性的克雷伯氏菌噬菌体的特征和基因组分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-11-15 DOI: 10.1007/s11262-024-02123-1

Shanzheng Bi, Hong Peng, Xiao Wei, Changjun Wang, Xiangna Zhao

引用次数: 0

Genetic characterization of a novel HIV-1 circulating recombinant form (CRF162_cpx) involving CRF01_AE, CRF07_BC and subtype B in Guangdong, China. 中国广东一种涉及CRF01_AE、CRF07_BC和B亚型的新型HIV-1循环重组形式（CRF162_cpx）的遗传特征

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.1007/s11262-024-02127-x

Yun Lan, Linghua Li, Mingfeng Xiao, Yaqing Lin, Xuemei Ling, Feng Li, Fengyu Hu

{"title":"Genetic characterization of a novel HIV-1 circulating recombinant form (CRF162_cpx) involving CRF01_AE, CRF07_BC and subtype B in Guangdong, China.","authors":"Yun Lan, Linghua Li, Mingfeng Xiao, Yaqing Lin, Xuemei Ling, Feng Li, Fengyu Hu","doi":"10.1007/s11262-024-02127-x","DOIUrl":"10.1007/s11262-024-02127-x","url":null,"abstract":"Human immunodeficiency virus type 1 (HIV-1) is characterized by its extremely high level of genetic diversity. The spread of different subtypes in the same population often leads to the emergence of circulating recombinant forms (CRFs) and unique recombinant forms (URFs). At present, the main recombinant subtypes of HIV-1 in China originate from CRF07_BC, CRF01_AE, CRF55_01B and subtype B. Here, we obtained the nearly full-length genomes (NFLGs) from eight HIV-1 infected patients in Guangdong Province, which shared highly similar recombinant patterns, involving two CRF01_AE, one CRF07_BC and two subtype B segments. The eight NFLG sequences own four similar breakpoints as follows: 1220 nucleotide (nt), 2243 nt, 2673 nt, and 5820 nt according to the HXB2 reference sequence, and they therefore were assigned as CRF162_cpx. This is the first complex CRF derived from CRF01_AE, CRF07_BC and subtype B in China. The Bayesian inference of the segments showed that HIV-1 CRF162_cpx was inferred to have approximately originated around 2010-2015. The emergence of CRF162_cpx indicates that the HIV diversity in southeast China constantly accumulates and evolves. Thus, intensive surveillance of HIV-1 molecular epidemiology should be reinforced.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"136-143"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The H5N1-NS1 protein affects the host cell cycle and apoptosis through interaction with the host lncRNA PIK3CD-AS2. H5N1-NS1 蛋白通过与宿主 lncRNA PIK3CD-AS2 相互作用，影响宿主细胞周期和细胞凋亡。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-18 DOI: 10.1007/s11262-024-02118-y

Man Zhang, Yingyue Zeng, Qingqing Liu, Feng Li, Jian Zhao, Zhikui Liu, Hongsheng Liu, Huawei Feng

{"title":"The H5N1-NS1 protein affects the host cell cycle and apoptosis through interaction with the host lncRNA PIK3CD-AS2.","authors":"Man Zhang, Yingyue Zeng, Qingqing Liu, Feng Li, Jian Zhao, Zhikui Liu, Hongsheng Liu, Huawei Feng","doi":"10.1007/s11262-024-02118-y","DOIUrl":"10.1007/s11262-024-02118-y","url":null,"abstract":"Long noncoding RNAs (lncRNAs) are involved in the host antiviral response, but how host lncRNAs interact with viral proteins remains unclear. The NS1 protein of avian influenza viruses can affect the interferon-dependent expression of several host lncRNAs, but the exact mechanism is unknown. To further investigate the molecular mechanism and functions of NS1 proteins and host lncRNAs, we performed RNA-immunoprecipitation sequencing assays on A549 cells transfected with the H5N1-NS1 gene. We identified multiple sets of host lncRNAs that interact with NS1. The results of the RNA pulldown assay indicated that PIK3CD-AS2 can directly interact with NS1 in vitro. Immunofluorescence confocal microscopy showed that these proteins were colocalized in the nucleus. Further studies revealed that PIK3CD-AS2 can also inhibit the transcription of NS1, which in turn affects the translation of the NS1 protein. PIK3CD-AS2 overexpression regulates NS1 protein-induced cell cycle arrest and initiates apoptosis. We hope this work will help elucidate the molecular mechanisms associated with NS1 proteins in the study of viral infections to promote the development of potential treatments for patients infected with avian influenza A viruses.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"38-53"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pooled screening for endogenous HHV-6 in subjects with coronary artery disease.

IF 1.9 4区医学

Virus Genes Pub Date : 2025-01-31 DOI: 10.1007/s11262-025-02134-6

Rie Koide, Yoshie Nakashima, Shohei Kojima, Yohei Sotomi, Yasushi Sakata, Yasuhiko Sakata, Kaoru Ito, Nicholas F Parrish

引用次数: 0

Molecular characterization and genetic analysis of highly pathogenic H5N1 clade 2.3.4.4b in seagulls from Dukan Lake, Iraq. 伊拉克杜坎湖海鸥高致病性H5N1进化枝2.3.4.4b的分子特征和遗传分析

IF 1.9 4区医学

Virus Genes Pub Date : 2025-01-22 DOI: 10.1007/s11262-025-02133-7

Mohammed Omar Baba Sheikh, Peshnyar M Atta Rashid, Zhino Hussein Rahim, Ari Salahadin Marouf, Star Sharif Saeed

{"title":"Molecular characterization and genetic analysis of highly pathogenic H5N1 clade 2.3.4.4b in seagulls from Dukan Lake, Iraq.","authors":"Mohammed Omar Baba Sheikh, Peshnyar M Atta Rashid, Zhino Hussein Rahim, Ari Salahadin Marouf, Star Sharif Saeed","doi":"10.1007/s11262-025-02133-7","DOIUrl":"https://doi.org/10.1007/s11262-025-02133-7","url":null,"abstract":"Avian influenza virus (AIV) remains a significant global threat, with periodic reemergence in Iraq. This study marks the first molecular characterization of the highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b in seagulls. The H5N1 AIV was identified during outbreaks in 2024 at Dukan Lake in Sulaimani province. Phylogenetic analysis of the HA gene revealed that the Dukan Lake strain belongs to subclade 2.3.4.4b, clustering closely with Kazakhstan HPAI strains (A/mute swan/Mangystau/1-S24R-2/2024(H5N1) and (A/Cygnus cygnus/Karakol lake/01/2024(H5N1)), with DNA identities of 99.38% and 98.82%, respectively. Genetic analysis showed a polybasic amino acid cleavage site motif (PLREKRRKRGLF) in the HA gene. Additionally, receptor-binding domain (RBD) analysis indicated a preference for the avian α-2, 3 Sialic acid (SA) receptor over the mammalian α-2, 6 SA receptor. The NA gene analysis revealed amino acid residues D199, I223, S247, and H275, which are susceptible to antiviral drugs. The molecular analysis of the H5N1 Dukan Lake seagull strain provides insights into how the virus spreads among different species and countries, which is crucial for global health security and the development of effective control measures.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of the whole‑genome sequence analysis of Fig badnavirus 2 from China. 中国乙型图病毒2全基因组序列分析首次报道。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-01-08 DOI: 10.1007/s11262-024-02132-0

Tuxunaili Aizitili, Yushanjiang Maimaiti, Zhixiang Zhang, Maihemuti Mijiti

{"title":"First report of the whole‑genome sequence analysis of Fig badnavirus 2 from China.","authors":"Tuxunaili Aizitili, Yushanjiang Maimaiti, Zhixiang Zhang, Maihemuti Mijiti","doi":"10.1007/s11262-024-02132-0","DOIUrl":"https://doi.org/10.1007/s11262-024-02132-0","url":null,"abstract":"A novel plant virus was identified in fig trees exhibiting ring spot symptoms through high-throughput sequencing (HTS). The complete genome sequence was successfully determined using PCR and RT-PCR techniques. The virus features a circular DNA genome of 7233 nucleotides (nt) in length, encompassing four open reading frames (ORFs). ORF1 and ORF2 encode hypothetical proteins, while ORF3 encodes a putative polyprotein with conserved domains, including a zinc finger, aspartic protease, reverse transcriptase (RT), and RNase H. ORF4 encodes a putative protein of unknown function. Comparative nucleotide sequence analysis of the RT + RNase H region reveals 84.46% and 78.82% identity with grapevine badnavirus 1 (GBV-1, MF781082.1) and fig badnavirus 1 (FBV-1, MK348055.1), respectively. Notably, this virus's genomic organization diverges from GBV-1 but is similar to FBV-1. Phylogenetic analysis demonstrates that the three isolates of this virus form a distinct clade within the badnaviruses. Based on genomic structure and phylogenetic relationships, this novel virus represents a new member of the genus Badnavirus and is proposed to be named \"Fig badnavirus 2\" (FBV-2).","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142958399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: The complete genomic sequence of Magnaporthe oryzae polymycovirus 1. 更正：米大孔菌多分枝病毒1的完整基因组序列。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-01-03 DOI: 10.1007/s11262-024-02129-9

Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng

引用次数: 0