Virus Genes最新文献_第10页

Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea 从根腐菌 Armillaria mellea 中分离出的新型丝状病毒的全长基因组特征描述

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-16 DOI: 10.1007/s11262-023-02041-8

Sıla Erkmen, Ergin Sahin, Ilgaz Akata

{"title":"Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea","authors":"Sıla Erkmen, Ergin Sahin, Ilgaz Akata","doi":"10.1007/s11262-023-02041-8","DOIUrl":"https://doi.org/10.1007/s11262-023-02041-8","url":null,"abstract":"Members of the genus Armillaria belong to the group of pathogenic and facultative saprotrophic fungi that are generally known as one of the causative agents of white root rot in infected plants including deciduous and evergreen trees and shrubs. Although several single-stranded RNA mycoviruses were previously described in different Armillaria species, there is no report on mitoviruses (one of the simplest RNA viruses of fungal hosts) known to infect Armillaria taxa. In this study, a new mitovirus denominated “Armillaria mellea mitovirus 1” (AmMV1) was identified in the sporophore samples of Armillaria mellea, commonly known as honey mushroom. AmMV1 has a genome length of 4440 nucleotides and a G + C content of 48%. It encompasses a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp). Comparison through BLASTp analysis revealed that the RdRp domain of AmMV1 shares a sequence identity ranging from 33.43% to 43.27% with RdRp domains of Duamitovirus genus members, having the highest similarity (43.27%) to Rhizoctonia solani mitovirus 94. According to phylogenetic analysis, AmMV1 is classified as a member of the genus Duamitovirus belonging to the Mitoviridae family. This marks the initial instance of a mitovirus identified in Armillaria spp..","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"12 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138690891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens 首次报告日本鸡禽类轮状病毒 A 的全基因组序列分析

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-15 DOI: 10.1007/s11262-023-02040-9

Ayana Okamoto, Hitoshi Takemae, Makoto Nagai, Shinichiro Hashimoto, Tetsuya Mizutani, Tetsuya Furuya

{"title":"First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens","authors":"Ayana Okamoto, Hitoshi Takemae, Makoto Nagai, Shinichiro Hashimoto, Tetsuya Mizutani, Tetsuya Furuya","doi":"10.1007/s11262-023-02040-9","DOIUrl":"https://doi.org/10.1007/s11262-023-02040-9","url":null,"abstract":"Rotavirus A infects many mammalian species, including humans and causes diarrhea and gastrointestinal diseases. The virus also infects various bird species, including chickens, although information of avian rotavirus A (ARVA) infection in chicken populations in Japan is scarce. In this study, we report for the first time the whole-genome sequences of ARVA strains from Japanese chicken populations. The virus strains were inoculated to MA104 cells and cultured viruses were used to obtain the sequences with the MiSeq system, and genetic analysis demonstrated the genotype constellation of G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8 of the Japanese chicken ARVA isolates. Phylogenetic analyses demonstrated that the VP1, VP2, VP3, VP4, VP7, NSP2, and NSP4 coding gene sequences of the Japanese strains were closer to those of Korean than the European ARVA strains, although such relationship was not clear for other genes. The data suggest that the Japanese ARVA strains and the ones in Korea have genetically close relationship, although the origin is not clear at this point. Further information including the whole-genome sequences of the Korean strains and sequences of other Japanese chicken ARVA strains will be necessary for elucidation of their origin.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"33 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138690894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aborted infection of human sodium taurocholate cotransporting polypeptide (hNTCP) expressing woodchuck hepatocytes with hepatitis B virus (HBV). 表达人牛磺胆酸钠协同转运多肽（hNTCP）的土拨鼠肝细胞感染乙型肝炎病毒（HBV）中止。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-09-20 DOI: 10.1007/s11262-023-02031-w

Lu Yang, Di Zhou, Kächele Martin, Jun Wu, Mingfa Chen, Mengji Lu, Dongliang Yang, Ulrike Protzer, Michael Roggendorf, Jingjiao Song

{"title":"Aborted infection of human sodium taurocholate cotransporting polypeptide (hNTCP) expressing woodchuck hepatocytes with hepatitis B virus (HBV).","authors":"Lu Yang, Di Zhou, Kächele Martin, Jun Wu, Mingfa Chen, Mengji Lu, Dongliang Yang, Ulrike Protzer, Michael Roggendorf, Jingjiao Song","doi":"10.1007/s11262-023-02031-w","DOIUrl":"10.1007/s11262-023-02031-w","url":null,"abstract":"Due to the limited host range of HBV, research progress has been hindered by the absence of a suitable animal model. The natural history of woodchuck hepatitis virus (WHV) infection in woodchuck closely mirrors that of HBV infection in human, making this species a promising candidate for establishing both in vivo and in vitro HBV infection models. Therefore, this animal may be a valuable species to evaluate HBV vaccines and anti-HBV drugs. A significant milestone in HBV and hepatitis D virus (HDV) infection is the discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the functional receptor. In an effort to enhance susceptibility to HBV infection, we introduced hNTCP into the woodchuck hepatocytes by multiple approaches including transduction of vLentivirus-hNTCP in woodchuck hepatocytes, transfection of p-lentivirus-hNTCP-eGFP plasmids into these cells, as well as transduction of vAdenovirus-hNTCP-eGFP. Encouragingly, our findings demonstrated the successful introduction of hNTCP into woodchuck hepatocytes. However, it was observed that these hNTCP-expressing hepatocytes were only susceptible to HDV infection but not HBV. This suggests the presence of additional crucial factors mediating early-stage HBV infection that are subject to stringent species-specific restrictions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"823-830"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of Xanthomonas phage M29, a new member of Foxunavirus isolated in the Czech Republic. 捷克分离的foxfoxavirus新成员黄单胞菌噬菌体M29的全基因组序列。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-09-04 DOI: 10.1007/s11262-023-02027-6

Mária Neoralová, Sára Brázdová, Aleš Eichmeier, Karel Petrzik

引用次数: 0

Design and validation of a laboratory-developed diagnostic assay for monkeypox virus. 设计和验证实验室开发的猴痘病毒诊断测定法。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-08-17 DOI: 10.1007/s11262-023-02024-9

Nikola Sklenovská, Mandy Bloemen, Valentijn Vergote, Anne-Sophie Logist, Bert Vanmechelen, Lies Laenen, Emmanuel André, Jean-Jacques Muyembe-Tamfum, Elke Wollants, Marc Van Ranst, Piet Maes, Tony Wawina-Bokalanga

{"title":"Design and validation of a laboratory-developed diagnostic assay for monkeypox virus.","authors":"Nikola Sklenovská, Mandy Bloemen, Valentijn Vergote, Anne-Sophie Logist, Bert Vanmechelen, Lies Laenen, Emmanuel André, Jean-Jacques Muyembe-Tamfum, Elke Wollants, Marc Van Ranst, Piet Maes, Tony Wawina-Bokalanga","doi":"10.1007/s11262-023-02024-9","DOIUrl":"10.1007/s11262-023-02024-9","url":null,"abstract":"Mpox is a viral zoonosis with endemic circulation in animals and humans in some West and Central African countries. The disease was imported a few times in the past to countries outside the African continent through infected animals or travelers, one of which resulted in an unprecedented global outbreak sustained by human-to-human transmission in 2022. Although timely and reliable diagnosis is a cornerstone of any disease control, availability of accurate diagnostic assays and comparative performance studies of diagnostic assays remains limited despite of the long-known identification of monkeypox virus (MPXV) as a human pathogen since 1970. We laboratory-developed a real-time PCR test (LDT) and evaluated its performance against the commercial TaqMan™ Monkeypox Virus Microbe Detection Assay (Applied Biosystems, Cat A50137). The limit of detection of the LDT was established at 1.2 genome copies/ml. The sensitivity and specificity of both assays were 99.14% and 100%, respectively, and both are capable of detecting both clade I and clade II of MPXV. Our results demonstrate the validity and accuracy of the LDT for confirmation of MPXV infection from lesion swabs samples.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"795-800"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10005544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dispersion and diversification of Lyssavirus rabies transmitted from haematophagous bats Desmodus rotundus: a phylogeographical study. 嗜血蝙蝠传播的狂犬病赖沙病毒的扩散和多样化：一项系统地理学研究。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-10-05 DOI: 10.1007/s11262-023-02030-x

Helena Beatriz de Carvalho Ruthner Batista, Luiz Fernando Pereira Vieira, Juliana Galera Castilho Kawai, Willian de Oliveira Fahl, Camila Mosca Barboza, Samira Achkar, Rafael de Novaes Oliveira, Paulo Eduardo Brandão, Pedro Carnieli Junior

{"title":"Dispersion and diversification of Lyssavirus rabies transmitted from haematophagous bats Desmodus rotundus: a phylogeographical study.","authors":"Helena Beatriz de Carvalho Ruthner Batista, Luiz Fernando Pereira Vieira, Juliana Galera Castilho Kawai, Willian de Oliveira Fahl, Camila Mosca Barboza, Samira Achkar, Rafael de Novaes Oliveira, Paulo Eduardo Brandão, Pedro Carnieli Junior","doi":"10.1007/s11262-023-02030-x","DOIUrl":"10.1007/s11262-023-02030-x","url":null,"abstract":"Rabies is worldwide zoonosis caused by Lyssavirus rabies (RABV) a RNA negative sense virus with low level of fidelity during replication cycle. Nucleoprotein of RABV is the most conserved between all five proteins of the virus and is the most used gene for phylogenetic and phylogeographic studies. Despite of rabies been very important in Public Health concern, it demands continuous prophylactic care for herbivores with economic interest, such as cattle and horses. The main transmitter of RABV for these animals in Brazil is the hematophagous bats Desmodus rotundus. The aim of this study was to determine the dispersion over time and space of RABV transmitted by D. rotundus. Samples of RABV from the State of São Paulo (SP), Southeast Brazil isolated from the central nervous system (CNS) of cattle, were submitted to RNA extraction, RT-PCR, sequencing and phylogeographic analyzes with BEAST (Bayesian Evolutionary Analysis Sampling Trees) v 2.5 software. Was possible to identify high rate of diversification in starts sublineages of RABV what are correlated with a behavior of D. rotundus, the main transmitter of rabies to cattle. This study also highlights the importance of continuous monitoring of genetic lineages of RABV in Brazil.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"817-822"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41151968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genomic analysis of a novel bacteriophage BUCT_49532 lysing Klebsiella pneumoniae. 裂解肺炎克雷伯菌的新型噬菌体BUCT_49532的特性和基因组分析。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-10-19 DOI: 10.1007/s11262-023-02033-8

Guangye Zhang, Yucong Liu, Jinhong Wang, Nan Li, Pengjun Han, Yiming Chen, Weijian Xu, Changxia Liu

{"title":"Characterization and genomic analysis of a novel bacteriophage BUCT_49532 lysing Klebsiella pneumoniae.","authors":"Guangye Zhang, Yucong Liu, Jinhong Wang, Nan Li, Pengjun Han, Yiming Chen, Weijian Xu, Changxia Liu","doi":"10.1007/s11262-023-02033-8","DOIUrl":"10.1007/s11262-023-02033-8","url":null,"abstract":"Bacteriophages are a type of virus widely distributed in nature that demonstrates a remarkable aptitude for selectively recognizing and infecting bacteria. In particular, Klebsiella pneumoniae is acknowledged as a clinical pathogen responsible for nosocomial infections and frequently develops multidrug resistance. Considering the increasing prevalence of antibiotic-resistant bacteria, bacteriophages have emerged as a compelling alternative therapeutic approach. In this study, a novel phage named BUCT_49532 was isolated from sewage using K. pneumoniae K1119 as the host. Electron microscopy revealed that BUCT_49532 belongs to the Caudoviricetes class. Further analysis through whole genome sequencing demonstrated that BUCT_49532 is a Jedunavirus comprised of linear double-stranded DNA with a length of 49,532 bp. Comparative genomics analysis based on average nucleotide identity (ANI) values revealed that BUCT_49532 should be identified as a novel species. Characterized by a good safety profile, high environmental stability, and strong lytic performance, phage BUCT_49532 presents an interesting case for consideration. Although its host range is relatively narrow, its application potential can be expanded by utilizing phage cocktails, making it a promising candidate for biocontrol approaches.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"852-867"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49684807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2. 牛病毒性腹泻病毒1 (BVDV-1)和BVDV-2适当亚型分型的新基因组靶点

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-08-17 DOI: 10.1007/s11262-023-02022-x

Carolina Isabela Mucellini, José Valter Joaquim Silva Júnior, Pablo Sebastian Britto de Oliveira, Rudi Weiblen, Eduardo Furtado Flores

{"title":"Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2.","authors":"Carolina Isabela Mucellini, José Valter Joaquim Silva Júnior, Pablo Sebastian Britto de Oliveira, Rudi Weiblen, Eduardo Furtado Flores","doi":"10.1007/s11262-023-02022-x","DOIUrl":"10.1007/s11262-023-02022-x","url":null,"abstract":"Whole-genome phylogenetic analysis, the most suitable strategy for subtyping bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2, is not feasible for many laboratories. Consequently, BVDV isolates/strains have been frequently subtyped based on analysis of single genomic regions, mainly the 5' untranslated region (UTR). This approach, however, may lead to inaccurate and/or poorly statistically supported viral classification. Herein, we describe novel primer sets whose amplicons may be easily sequenced and used for BVDV subtyping. Initially, genomic regions previously described as the most suitable targets for BVDV subtyping were analyzed for design of high-coverage primers. The putative amplicons were analyzed in silico for their suitability to reproduce the phylogenetic classification of 118 BVDV-1 and 88 BVDV-2 complete/near-complete genomes (CNCGs) (GenBank). This analysis was also performed considering the region amplifiable by primers HCV90-368, 324-326 and BP189-389 (5'UTR), which have been used for BVDV diagnosis and/or classification. After confirming the agreement between the analyses of our primers' amplicon versus the CNCGs, we optimized the RT-PCRs and evaluated their performance for amplification of BVDV isolates/strains (n = 35 for BVDV-1; n = 33 for BVDV-2). Among the potential targets for BVDV subtyping, we designed high-coverage primers for NS3-NS4A (BVDV-1) (526 bp amplicon) and NS5B (BVDV-2) (728 bp). The classification based on these regions fully reproduced the subtyping of all CNCGs. On the other hand, subtyping based on the putative amplicons from primers HCV90-368, 324-326 and BP189-389 showed disagreements in relation the CNCG analysis. The NS3-NS4A and NS5B primers also allowed the amplification of all BVDV isolates/strains tested. Finally, we suggest the use of these primers in future phylogenetic and epidemiological studies of BVDVs.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"836-844"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10017224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SCD1 inhibits HBV replication by regulating autophagy under high lipid conditions. 在高脂条件下，SCD1通过调节自噬抑制HBV复制。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-08-29 DOI: 10.1007/s11262-023-02028-5

Xuan Du, Xiaoyi Shi, Mei Han, Xiaoyun Gao, Chuang Wang, Chunmeng Jiang, Chunwen Pu

{"title":"SCD1 inhibits HBV replication by regulating autophagy under high lipid conditions.","authors":"Xuan Du, Xiaoyi Shi, Mei Han, Xiaoyun Gao, Chuang Wang, Chunmeng Jiang, Chunwen Pu","doi":"10.1007/s11262-023-02028-5","DOIUrl":"10.1007/s11262-023-02028-5","url":null,"abstract":"Chronic hepatitis B virus (HBV) infection remains a significant public health concern worldwide. Several metabolic processes regulate HBV DNA replication, including autophagy and lipid metabolism. In this study, we clarified the effect of lipids on HBV replication and elucidated possible mechanisms. We discovered that lipid metabolic gene expression levels were negatively correlated with the HBV DNA in plasma. Our data showed that fatty acid stimulation significantly reduced HBV DNA, hepatitis B surface antigen (HBsAg), and hepatitis B e antigen (HBeAg) levels in HepG2.2.15 cells, which are human hepatoma cell cultures transfected with HBV DNA. The Stearoyl coenzyme A desaturase 1 (SCD1)-autophagy pathway has also been implicated in inhibiting HBV replication by fatty acids stimulation. SCD1 knockdown deregulates the inhibitory effect of fatty acids on HBV by enhancing autophagy. When 3 methyladenine (3MA) was added, the inhibitory effects of specific autophagy inhibitors eliminated the positive effects of SCD1 knockdown on HBV replication. Our results indicate that SCD1 participates in the regulation of inhibition of HBV replication by fatty acids stimulation through regulating autophagy.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"801-816"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10104248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genome evolution of Marek's disease viruses in chickens and turkeys in China. 中国鸡和火鸡马立克氏病病毒的基因组进化。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-10-18 DOI: 10.1007/s11262-023-02034-7

Wen Li, Huifang Meng, Xianghui Liang, Jinyu Peng, David M Irwin, Xuejuan Shen, Yongyi Shen

{"title":"The genome evolution of Marek's disease viruses in chickens and turkeys in China.","authors":"Wen Li, Huifang Meng, Xianghui Liang, Jinyu Peng, David M Irwin, Xuejuan Shen, Yongyi Shen","doi":"10.1007/s11262-023-02034-7","DOIUrl":"10.1007/s11262-023-02034-7","url":null,"abstract":"The virus that causes Marek's disease (MD) is globally ubiquitous in chickens, continuously evolving, and poses a significant threat to the poultry industry. Although vaccines are extensively used, MD still occurs frequently and the virus has evolved increased virulence in China. Here, we report an outbreak of MD in vaccinated chickens and unvaccinated turkeys in a backyard farm in Guangdong province, China, in 2018. Phylogenetic analysis revealed two lineages of MDVs at this farm, with one lineage, containing isolates from two turkeys and five chickens, clustering with virulent Chinese strains and displays a relatively high genetic divergence from the vaccine strains. These new isolates appear to have broken through vaccine immunity, yielding this outbreak of MD in chickens and turkeys. The second lineage included four chicken isolates that clustered with the CVI988 and 814 vaccine strains. The large diversity of MDVs in this single outbreak reveals a complex circulation of MDVs in China. Poor breeding conditions and the weak application of disease prevention and control measures make backyard farms a hotbed for the evolution of viruses that cause infectious diseases. This is especially important in MDV as the MD vaccines do not provide sterilizing immunity, which allows the replication and shedding of virulent field viruses by vaccinated individuals and supporting the continuous evolution of MDVs. Hence, constant monitoring of the evolution of MDVs is necessary to understand the evolution of these field viruses and potential expansions of their host range.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"845-851"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41240753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0