Virus Genes最新文献_第10页

Molecular functions of HAX1 during disease progress. 疾病进展过程中 HAX1 的分子功能

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-11 DOI: 10.1007/s11262-024-02081-8

Dajun Zhang, Jinke Yang, Qi Huang, Dengshuai Zhao, Tianyu Wang, Dixi Yu, Limei Qin, Keshan Zhang

引用次数: 0

Immunization of recombinant NS3 protein (protease region) of dengue virus induces high levels of CTLA-4 and apoptosis in splenocytes of BALB/c mice. 登革病毒重组 NS3 蛋白（蛋白酶区）免疫可诱导高水平 CTLA-4 和 BALB/c 小鼠脾细胞凋亡。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI: 10.1007/s11262-024-02095-2

Víctor Adolfo Romero-Cruz, Angel Ramos-Ligonio, Karen García-Alejandro, Melissa Cerecedo-García, María de la Soledad Lagunes-Castro, Aracely López-Monteon

{"title":"Immunization of recombinant NS3 protein (protease region) of dengue virus induces high levels of CTLA-4 and apoptosis in splenocytes of BALB/c mice.","authors":"Víctor Adolfo Romero-Cruz, Angel Ramos-Ligonio, Karen García-Alejandro, Melissa Cerecedo-García, María de la Soledad Lagunes-Castro, Aracely López-Monteon","doi":"10.1007/s11262-024-02095-2","DOIUrl":"10.1007/s11262-024-02095-2","url":null,"abstract":"DENV infection outcomes depend on the host's variable expression of immune receptors and mediators, leading to either resolution or exacerbation. While the NS3 protein is known to induce robust immune responses, the specific impact of its protease region epitopes remains unclear. This study investigated the effect of recombinant NS3 protease region proteins from all four DENV serotypes on splenocyte activation in BALB/c mice (n = 5/group). Mice were immunized with each protein, and their splenocytes were subsequently stimulated with homologous antigens. We measured the expression of costimulatory molecules (CD28, CD80, CD86, CD152) by flow cytometry, along with IL-2 production, CD25 expression, and examined the antigen-specific activation of CD4 + and CD8 + T cells. Additionally, the expression of IL-1, IL-10, and TGF-β1 in splenocytes from immunized animals was assessed. Apoptosis was evaluated using Annexin V/PI staining and DNA fragmentation analysis. Stimulation of splenocytes from immunized mice triggered apoptosis (phosphatidylserine exposure and caspase 3/7 activation) and increased costimulatory molecule expression, particularly CD152. Low IL-2 production and low CD25 expression, as well as sustained expression of the IL-10 gene. These results suggest that these molecules might be involved in mechanisms by which the NS3 protein contributes to viral persistence and disease pathogenesis.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"475-487"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of two novel complete genomes of myxoma virus vaccine strains. 肌瘤病毒疫苗株两种新型完整基因组的比较分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-11 DOI: 10.1007/s11262-024-02090-7

Sofya Volodina, Ilya Titov, Sergey Zhivoderov, Sergey Yurkov, Alexander Malogolovkin

引用次数: 0

Isolation, characterization, and potential application of Acinetobacter baumannii phages against extensively drug-resistant strains 鲍曼不动杆菌噬菌体的分离、表征和潜在应用，以对抗广泛耐药菌株

IF 1.6 4区医学

Virus Genes Pub Date : 2024-09-10 DOI: 10.1007/s11262-024-02103-5

Sanaz Rastegar, Mikael Skurnik, Hira Niaz, Omid Tadjrobehkar, Ali Samareh, Hossein Hosseini-Nave, Salehe Sabouri

{"title":"Isolation, characterization, and potential application of Acinetobacter baumannii phages against extensively drug-resistant strains","authors":"Sanaz Rastegar, Mikael Skurnik, Hira Niaz, Omid Tadjrobehkar, Ali Samareh, Hossein Hosseini-Nave, Salehe Sabouri","doi":"10.1007/s11262-024-02103-5","DOIUrl":"https://doi.org/10.1007/s11262-024-02103-5","url":null,"abstract":"One of the significant issues in treating bacterial infections is the increasing prevalence of extensively drug-resistant (XDR) strains of Acinetobacter baumannii. In the face of limited or no viable treatment options for extensively drug-resistant (XDR) bacteria, there is a renewed interest in utilizing bacteriophages as a treatment option. Three Acinetobacter phages (vB_AbaS_Ftm, vB_AbaS_Eva, and vB_AbaS_Gln) were identified from hospital sewage and analyzed for their morphology, host ranges, and their genome sequences were determined and annotated. These phages and vB_AbaS_SA1 were combined to form a phage cocktail. The antibacterial effects of this cocktail and its combinations with selected antimicrobial agents were evaluated against the XDR A. baumannii strains. The phages exhibited siphovirus morphology. Out of a total of 30 XDR A. baumannii isolates, 33% were sensitive to vB_AbaS_Ftm, 30% to vB_AbaS_Gln, and 16.66% to vB_AbaS_Eva. When these phages were combined with antibiotics, they demonstrated a synergistic effect. The genome sizes of vB_AbaS_Ftm, vB_AbaS_Eva, and vB_AbaS_Gln were 48487, 50174, and 50043 base pairs (bp), respectively, and showed high similarity. Phage cocktail, when combined with antibiotics, showed synergistic effects on extensively drug-resistant (XDR) strains of A. baumannii. However, the need for further study to fully understand the mechanisms of action and potential limitations of using these phages is highlighted.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"68 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stearoyl-CoA desaturase 1 is targeted by EBV-encoded miR-BART20-5p and regulates cell autophagy, proliferation, and migration in EBV-associated gastric cancer 硬脂酰-CoA 去饱和酶 1 是 EBV 编码的 miR-BART20-5p 的靶标，在 EBV 相关胃癌中调控细胞自噬、增殖和迁移

IF 1.6 4区医学

Virus Genes Pub Date : 2024-08-03 DOI: 10.1007/s11262-024-02094-3

Zhiyuan Gong, Duo Shi, Zhiyong Yan, Lingling Sun, Wen Liu, Bing Luo

引用次数: 0

White yam (Dioscorea rotundata) plants exhibiting virus-like symptoms are co-infected with a new potyvirus and a new crinivirus in Ethiopia. 埃塞俄比亚的白山药（Dioscorea rotundata）植物表现出病毒样症状，同时感染了一种新的壶状病毒和一种新的冠状病毒。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-06-04 DOI: 10.1007/s11262-024-02077-4

Ashebir Gogile, Dennis Knierim, Paolo Margaria, Wulf Menzel, Mereme Abide, Misrak Kebede, Dawit Kidanemariam, Adane Abraham

{"title":"White yam (Dioscorea rotundata) plants exhibiting virus-like symptoms are co-infected with a new potyvirus and a new crinivirus in Ethiopia.","authors":"Ashebir Gogile, Dennis Knierim, Paolo Margaria, Wulf Menzel, Mereme Abide, Misrak Kebede, Dawit Kidanemariam, Adane Abraham","doi":"10.1007/s11262-024-02077-4","DOIUrl":"10.1007/s11262-024-02077-4","url":null,"abstract":"White yam (Dioscorea rotundata) plants collected from farmers' fields and planted at the Areka Agricultural Research Center, Southern Ethiopia, displayed mosaic, mottling, and chlorosis symptoms. To determine the presence of viral pathogens, an investigation for virome characterization was conducted by Illumina high-throughput sequencing. The bioinformatics analysis allowed the assembly of five viral genomes, which according to the ICTV criteria were assigned to a novel potyvirus (3 genome sequences) and a novel crinivirus (2 genome sequences). The potyvirus showed ~ 66% nucleotide (nt) identity in the polyprotein sequence to yam mosaic virus (NC004752), clearly below the demarcation criteria of 76% identity. For the crinivirus, the RNA 1 and RNA 2 shared the highest sequence identity to lettuce chlorosis virus, and alignment of the aa sequence of the RdRp, CP and HSP70h (~ 49%, 45% and 76% identity), considered for the demarcation criteria, revealed the finding of a novel virus species. The names Ethiopian yam virus (EYV) and Yam virus 1 (YV-1) are proposed for the two tentative new virus species.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"423-433"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141237729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The full genome characterization of avian encephalomyelitis virus, Iran: a vertical transmission case. 伊朗禽脑脊髓炎病毒的全基因组特征：一个垂直传播案例。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI: 10.1007/s11262-024-02076-5

Arash Ghalyanchilangeroudi, Seyed Ahmad Madani, Hamideh Najafi, Zahra Ziafati Kafi, Naser Sadri, Soroush Sarmadi, Omid Eghbali, Fahimeh Jamiri, Alireza Bakhshi, Hossein Hosseini

{"title":"The full genome characterization of avian encephalomyelitis virus, Iran: a vertical transmission case.","authors":"Arash Ghalyanchilangeroudi, Seyed Ahmad Madani, Hamideh Najafi, Zahra Ziafati Kafi, Naser Sadri, Soroush Sarmadi, Omid Eghbali, Fahimeh Jamiri, Alireza Bakhshi, Hossein Hosseini","doi":"10.1007/s11262-024-02076-5","DOIUrl":"10.1007/s11262-024-02076-5","url":null,"abstract":"Avian encephalomyelitis (AE) is an important infectious poultry disease worldwide that is caused by avian encephalomyelitis virus (AEV). The causative virus can be transmitted both horizontally and vertically. In the present study, an AEV suspected outbreak with typical neurological signs occurred in broilers. Histopathological examination, RT-PCR assay and full genome sequencing were applied to confirm the presence of AEV. Phylogenetic analysis of the full genome sequence showed that the detected AEV strain at 7055 nucleotide length is classified in cluster I and is closely related to vaccinal USA and China originated isolates. Although, the outbreaks of AE in progeny of vaccinated breeders have been reported previously, the source of infection was unknown. Based on the results obtained in this study, the outbreaks are vaccine-originated. This study provides the first whole genome analysis of AEV from Iran and reveals that the AEV possesses a hepatitis C virus-like internal ribosome entry site.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"393-401"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of four isomers of the human cytomegalovirus genome using nanopore long-read sequencing. 利用纳米孔长读数测序技术检测人类巨细胞病毒基因组的四种异构体。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-06-11 DOI: 10.1007/s11262-024-02083-6

Hideaki Nanamiya, Daisuke Tanaka, Gen Hiyama, Takao Isogai, Shinya Watanabe

引用次数: 0

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea. 两种新型镰刀菌病毒共同感染单一植物病原真菌 Botrytis cinerea 分离物的特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1007/s11262-024-02073-8

Aqeel Ahmed, Haris Ahmed Khan, Atif Jamal, Nasar Virk, Muhammad Faraz Bhatti

{"title":"Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea.","authors":"Aqeel Ahmed, Haris Ahmed Khan, Atif Jamal, Nasar Virk, Muhammad Faraz Bhatti","doi":"10.1007/s11262-024-02073-8","DOIUrl":"10.1007/s11262-024-02073-8","url":null,"abstract":"A wide diversity of mycoviruses has been reported from Botrytis species, some with the potential to suppress the pathogenic abilities of this fungus. Considering their importance, this study was devised to find potential hypovirulence-associated mycoviruses found in Botrytis cinerea strains isolated from Pakistani strawberry fields. Here we report the complete genome characterization of two fusariviruses co-infecting a single isolate of phytopathogenic fungus B. cinerea (Kst14a). The viral genomes were sequenced by deep sequencing using total RNA fractions of the Kst14a isolate. The identified viruses were tentatively named Botrytis cinerea fusarivirus 9 (BcFV9) and Botrytis cinerea fusarivirus 3a (BcFV3a). Both viruses had a single-segmented (ssRNA) genome having a size of 6424 and 8370 nucleotides encoding two discontinuous open reading frames (ORFs). ORF-1 of both mycoviruses encodes for a polyprotein having a conserved domain of RNA-dependent RNA polymerase (RdRP) and a helicase domain (Hel) which function in RNA replication, while ORF2 encodes a hypothetical protein with an unknown function, respectively. Phylogenetic analysis indicated that BcFV9 made a clade with the genus Alphafusarivirus and BcFV3a fall in the genus Betafusarivirus in the family Fusariviridae. To our knowledge, this is the first report of two fusariviruses identified in isolates of B. cinerea from Pakistan. Both mycoviruses successfully transfected to a compatible strain of B. cinerea (Mst11). A comparison of virus-free (VF) and virus-infected (VI) isogenic lines showed the presence of these viruses was causing hypovirulence in infected strains. Virus-infected strains also had a small lesion size while testing the pathogenicity via apple assay.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"402-411"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140877864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of human astrovirus infection in children under 5 years of age with acute gastroenteritis in Tehran, Iran, 2021-2022: co-infection with rotavirus. 2021-2022 年伊朗德黑兰 5 岁以下急性肠胃炎儿童感染人类星状病毒的分子特征：与轮状病毒合并感染。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1007/s11262-024-02075-6

Atefeh Kachooei, Mahtab Mirhoseinian, Somayeh Jalilvand, Tayebeh Latifi, Mahsa Feizi, Zahra Shahosseini, Arash Arashkia, Sayed Mahdi Marashi, Zabihollah Shoja

{"title":"Molecular characterization of human astrovirus infection in children under 5 years of age with acute gastroenteritis in Tehran, Iran, 2021-2022: co-infection with rotavirus.","authors":"Atefeh Kachooei, Mahtab Mirhoseinian, Somayeh Jalilvand, Tayebeh Latifi, Mahsa Feizi, Zahra Shahosseini, Arash Arashkia, Sayed Mahdi Marashi, Zabihollah Shoja","doi":"10.1007/s11262-024-02075-6","DOIUrl":"10.1007/s11262-024-02075-6","url":null,"abstract":"Human astroviruses (HAstVs) are considered important causative pathogens of acute gastroenteritis (AGE) in children under 5 years of age worldwide, along with group A rotavirus (RVA), norovirus (NoV), and enteric adenovirus (EAdV). The present study was aimed to both detect HAstV and its co-infections and investigate genetic analysis of circulating HAstV and co-infected virus in hospitalized children under 5 years of age with AGE in Iran. Accordingly, a sum of 200 stool specimens were screened by PCR for HAstV during 2021-2022. The HAstV was found in 0.5% of 200 specimens (n = 1) while was co-infected with RVA. The genetic and phylogenetic analysis indicated HAstV1 genotype, which clustered with viruses from lineage 1b, which has not been previously reported in Iran. The detected RVA strain belonged to G1 lineage II/P[8]-lineage III, which has been reported previously in Iran as the most common strain. The further genetic analysis of RVA VP6 and NSP4 demonstrated an atypical genotype pattern G1P[8]-I1-E2, as a mono-reassortant of a Wa-like genogroup, which appeared to be reassorted with the NSP4 gene of E2 genotype of the G2P[4] DS-1 genogroup. Although the clinical outcomes of the AGE-causing viruses co-infection is not yet entirely clear, it seems that future studies will be helpful to merge clinical and epidemiological data of co-infecting viruses for a more accurate medical and clinical relevance in symptomatic children.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"357-369"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0