Virus Genes最新文献_第10页

P4 protein of an Indian isolate of rice tungro bacilliform virus modulates gene silencing. 水稻桐花叶病毒印度分离株的 P4 蛋白调节基因沉默。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-02-01 Epub Date: 2023-12-06 DOI: 10.1007/s11262-023-02039-2

Madhvi Naresh, Arunima Purkayastha, Indranil Dasgupta

{"title":"P4 protein of an Indian isolate of rice tungro bacilliform virus modulates gene silencing.","authors":"Madhvi Naresh, Arunima Purkayastha, Indranil Dasgupta","doi":"10.1007/s11262-023-02039-2","DOIUrl":"10.1007/s11262-023-02039-2","url":null,"abstract":"Plant hosts and their viral pathogens are engaged in a constant cycle of defense and counter-defense as part of a molecular arms race, principal among them being the plant RNAi defense and the viral RNAi suppressor counter-defense. Rice tungro bacilliform virus (RTBV), member of the family Caulimoviridae, genus Tungrovirus, species Tungrovirus oryzae, infects rice in South- and Southeast Asia and causes severe symptoms of stunting, yellow-orange discoloration and twisting of leaf tips. To better understand the possible counter-defensive roles of RTBV against the host RNAi defense system, we explored the ability of the P4 protein of an Indian isolate of RTBV to act as a possible modulator of RNAi. Using a transient silencing and silencing suppression assay in Nicotiana benthamiana, we show that P4 not only displays an RNAi suppressor function, but also potentially enhances RNAi. The results also suggests that the N-terminal 168 amino acid residues of P4 are sufficient to maintain RNAi suppressor activity. Taken together with the earlier reports this work strengthens the view that the P4 protein carries out RNAi suppressor and a potential RNAi enhancer function.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"55-64"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138489047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of bactericidal effects of bacteriophage and gentamicin combination regimen against Staphylococcus aureus and Pseudomonas aeruginosa strains in a mice diabetic wound model. 在小鼠糖尿病伤口模型中增强噬菌体和庆大霉素联合疗法对金黄色葡萄球菌和铜绿假单胞菌的杀菌效果。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-02-01 Epub Date: 2023-12-11 DOI: 10.1007/s11262-023-02037-4

Javad Jokar, Hussein T Abdulabbas, Kazem Javanmardi, Mohammad Ali Mobasher, Shima Jafari, Abdolmajid Ghasemian, Niloofar Rahimian, Ali Zarenezhad, Ava ُSoltani Hekmat

{"title":"Enhancement of bactericidal effects of bacteriophage and gentamicin combination regimen against Staphylococcus aureus and Pseudomonas aeruginosa strains in a mice diabetic wound model.","authors":"Javad Jokar, Hussein T Abdulabbas, Kazem Javanmardi, Mohammad Ali Mobasher, Shima Jafari, Abdolmajid Ghasemian, Niloofar Rahimian, Ali Zarenezhad, Ava ُSoltani Hekmat","doi":"10.1007/s11262-023-02037-4","DOIUrl":"10.1007/s11262-023-02037-4","url":null,"abstract":"Diabetic patients are more susceptible to developing wound infections resulting in poor and delayed wound healing. Bacteriophages, the viruses that target-specific bacteria, can be used as an alternative to antibiotics to eliminate drug-resistant bacterial infections. Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) are among the most frequently identified pathogens in diabetic foot ulcers (DFUs). The aim of this study was assessment of bacteriophage and gentamicin combination effects on bacterial isolates from DFU infections. Specific bacteriophages were collected from sewage and animal feces samples and the phages were enriched using S. aureus and P. aeruginosa cultures. The lytic potential of phage isolates was assessed by the clarity of plaques. We isolated and characterized four lytic phages: Stp2, Psp1, Stp1, and Psp2. The phage cocktail was optimized and investigated in vitro. We also assessed the effects of topical bacteriophage cocktail gel on animal models of DFU. Results revealed that the phage cocktail significantly reduced the mortality rate in diabetic infected mice. We determined that treatment with bacteriophage cocktail effectively decreased bacterial colony counts and improved wound healing in S. aureus and P. aeruginosa infections, especially when administrated concomitantly with gentamicin. The application of complementary therapy using a phage cocktail and gentamicin, could offer an attractive approach for the treatment of wound diabetic bacterial infections.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"80-96"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138806423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel bluetongue virus serotype 2 strain isolated from a farmed Florida white-tailed deer (Odocoileus virginianus) arose from reassortment of gene segments derived from co-circulating serotypes in the Southeastern USA. 从养殖的佛罗里达白尾鹿（Odocoileus virginianus）身上分离出的新型蓝舌病病毒血清型 2 株系是从美国东南部共同流行的血清型中提取的基因片段重组而来。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-02-01 Epub Date: 2024-01-05 DOI: 10.1007/s11262-023-02047-2

Pedro H O Viadanna, Austin Surphlis, An-Chi Cheng, Catherine E Dixon, Sarah Meisner, Kristen N Wilson, Zoe S White, Emily DeRuyter, Tracey D Logan, Juan M C Krauer, John A Lednicky, Samantha M Wisely, Kuttichantran Subramaniam

{"title":"A novel bluetongue virus serotype 2 strain isolated from a farmed Florida white-tailed deer (Odocoileus virginianus) arose from reassortment of gene segments derived from co-circulating serotypes in the Southeastern USA.","authors":"Pedro H O Viadanna, Austin Surphlis, An-Chi Cheng, Catherine E Dixon, Sarah Meisner, Kristen N Wilson, Zoe S White, Emily DeRuyter, Tracey D Logan, Juan M C Krauer, John A Lednicky, Samantha M Wisely, Kuttichantran Subramaniam","doi":"10.1007/s11262-023-02047-2","DOIUrl":"10.1007/s11262-023-02047-2","url":null,"abstract":"Bluetongue disease is a reportable animal disease that affects wild and farmed ruminants, including white-tailed deer (WTD). This report documents the clinical findings, ancillary diagnostics, and genomic characterization of a novel reassortant bluetongue virus serotype 2 (BTV-2) strain isolated from a dead Florida farmed WTD in 2022. Our analyses support that this BTV-2 strain likely stemmed from the acquisition of genome segments from co-circulating BTV strains in Florida and Louisiana. In addition, our analyses also indicate that genetically uncharacterized BTV strains may be circulating in the Southeastern USA; however, the identity and reassortant status of these BTV strains cannot be determined based on the VP2 and VP5 genome sequences. Hence, continued surveillance based on complete genome characterization is needed to understand the genetic diversity of BTV strains in this region and the potential threat they may pose to the health of deer and other ruminants.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"100-104"},"PeriodicalIF":1.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139106895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shrimp classification for white spot syndrome detection through enhanced gated recurrent unit-based wild geese migration optimization algorithm 通过基于大雁迁徙优化算法的增强型门控递归单元进行白斑综合征检测的虾类分类

IF 1.6 4区医学

Virus Genes Pub Date : 2024-01-22 DOI: 10.1007/s11262-023-02049-0

L. Ramachandran, S. P. Mangaiyarkarasi, A. Subramanian, S. Senthilkumar

{"title":"Shrimp classification for white spot syndrome detection through enhanced gated recurrent unit-based wild geese migration optimization algorithm","authors":"L. Ramachandran, S. P. Mangaiyarkarasi, A. Subramanian, S. Senthilkumar","doi":"10.1007/s11262-023-02049-0","DOIUrl":"https://doi.org/10.1007/s11262-023-02049-0","url":null,"abstract":"The major dangerous viral infection for cultivated shrimps is WSSV. The virus is extremely dangerous, spreads swiftly, and may result in up to 100% mortality in 3–10 days. The vast wrapped double stranded DNA virus known as WSSV describes a member of the Nimaviridae viral family’s species Whispovirus. It impacts a variety of crustacean hosts but predominantly marine shrimp species that are raised for commercial purposes. The entire age groups are affected by the virus, which leads to widespread mortality. Mesodermal and ectodermal tissues, like the lymph nodes, gills, and cuticular epithelium, represents the centres of infection. Complete genome sequencing related to the WSSV strains from Thailand, China, and Taiwan has identified minute genetic variations amongst them. There exist conflicting findings on the causes of WSSV pathogenicity, which involve variations in the size associated with the genome, the count of tandem repeats, and the availability or lack of certain proteins. Hence, this paper plans to perform the shrimp classification for the WSSV on the basis of novel deep learning methodology. Initially, the data is gathered from the farms as well as internet sources. Next, the pre-processing of the gathered shrimp images is accomplished using the LBP technique. These pre-processed images undergo the segmentation process utilizing the TGVFCMS approach. The extraction of the features from these segmented images is performed by the PLDA technique. In the final step, the classification of the shrimp into healthy shrimp and WSSV affected shrimp is done by the EGRU, in which the parameter tuning is accomplished by the wild GMO algorithm with the consideration of accuracy maximization as the major objective function. Performance indicators for accuracy have been compared with those of various conventional methods, and the results show that the methodology is capable of accurately identifying the shrimp WSSV illness.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"138 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139518659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence analysis of the Spike, RNA-dependent RNA polymerase, and protease genes reveals a distinct evolutionary pattern of SARS-CoV-2 variants circulating in Yogyakarta and Central Java provinces, Indonesia 对 Spike、RNA 依赖性 RNA 聚合酶和蛋白酶基因的序列分析表明，在印度尼西亚日惹省和中爪哇省流行的 SARS-CoV-2 变种具有不同的进化模式

IF 1.6 4区医学

Virus Genes Pub Date : 2024-01-20 DOI: 10.1007/s11262-023-02048-1

Mohamad Saifudin Hakim, Gunadi, Ayu Rahayu, Hendra Wibawa, Laudria Stella Eryvinka, Endah Supriyati, Khanza Adzkia Vujira, Kristy Iskandar, Afiahayati, Edwin Widyanto Daniwijaya, Farida Nur Oktoviani, Luthvia Annisa, Fadila Dyah Trie Utami, Verrell Christopher Amadeus, Setiani Silvy Nurhidayah, Tiara Putri Leksono, Fiqih Vidiantoro Halim, Eggi Arguni, Titik Nuryastuti, Tri Wibawa

{"title":"Sequence analysis of the Spike, RNA-dependent RNA polymerase, and protease genes reveals a distinct evolutionary pattern of SARS-CoV-2 variants circulating in Yogyakarta and Central Java provinces, Indonesia","authors":"Mohamad Saifudin Hakim, Gunadi, Ayu Rahayu, Hendra Wibawa, Laudria Stella Eryvinka, Endah Supriyati, Khanza Adzkia Vujira, Kristy Iskandar, Afiahayati, Edwin Widyanto Daniwijaya, Farida Nur Oktoviani, Luthvia Annisa, Fadila Dyah Trie Utami, Verrell Christopher Amadeus, Setiani Silvy Nurhidayah, Tiara Putri Leksono, Fiqih Vidiantoro Halim, Eggi Arguni, Titik Nuryastuti, Tri Wibawa","doi":"10.1007/s11262-023-02048-1","DOIUrl":"https://doi.org/10.1007/s11262-023-02048-1","url":null,"abstract":"During the Covid-19 pandemic, the resurgence of SARS-CoV-2 was due to the development of novel variants of concern (VOC). Thus, genomic surveillance is essential to monitor continuing evolution of SARS-CoV-2 and to track the emergence of novel variants. In this study, we performed phylogenetic, mutation, and selection pressure analyses of the Spike, nsp12, nsp3, and nsp5 genes of SARS-CoV-2 isolates circulating in Yogyakarta and Central Java provinces, Indonesia from May 2021 to February 2022. Various bioinformatics tools were employed to investigate the evolutionary dynamics of distinct SARS-CoV-2 isolates. During the study period, 213 and 139 isolates of Omicron and Delta variants were identified, respectively. Particularly in the Spike gene, mutations were significantly more abundant in Omicron than in Delta variants. Consistently, in all of four genes studied, the substitution rates of Omicron were higher than that of Delta variants, especially in the Spike and nsp12 genes. In addition, selective pressure analysis revealed several sites that were positively selected in particular genes, implying that these sites were functionally essential for virus evolution. In conclusion, our study demonstrated a distinct evolutionary pattern of SARS-CoV-2 variants circulating in Yogyakarta and Central Java provinces, Indonesia.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"9 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139510028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A virus from Aspergillus cibarius with features of alpha- and betachrysoviruses 一种来自西巴里曲霉的病毒，具有甲型和乙型鲎病毒的特征

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-30 DOI: 10.1007/s11262-023-02043-6

Tobias Lutz, Gitta Jutta Langer, Cornelia Heinze

{"title":"A virus from Aspergillus cibarius with features of alpha- and betachrysoviruses","authors":"Tobias Lutz, Gitta Jutta Langer, Cornelia Heinze","doi":"10.1007/s11262-023-02043-6","DOIUrl":"https://doi.org/10.1007/s11262-023-02043-6","url":null,"abstract":"From the ascomycete Aspergillus cibarius strain NW-FVA 2590, which was originally isolated from a root, associated with stem collar necrosis of Fraxinus excelsior L., a novel virus was isolated and characterized. Its genome is encoded on three monocistronic dsRNA segments ranging from 3683 bp (dsRNA 1) over 3093 (dsRNA 2) to 2902 bp (dsRNA 3), which are packed in isometric particles of around 35 nm. While the viral RdRp (P1) is encoded on segment 1, protein sequencing showed that two more structural proteins are present which are translated from dsRNA 2 (P2) and dsRNA 3 (P3) and possibly form the viral capsid. Additionally, P2 and P3 may undergo posttranslational modifications since the detected proteins bands deviated from the calculated sizes. Due to its phylogenetic position, the novel virus was grouped in the family of Chrysoviridae and was tentatively denominated as Aspergillus cibarius chrysovirus 1 (AcCV1). Due to its composition, biological properties and phylogenetic position, distant from the genera Alphachrysovirus and Betachrysovirus, we suggest to position AcCV1 in a proposed genus “Gammachrysovirus”.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"46 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea 从根腐菌 Armillaria mellea 中分离出的新型丝状病毒的全长基因组特征描述

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-16 DOI: 10.1007/s11262-023-02041-8

Sıla Erkmen, Ergin Sahin, Ilgaz Akata

{"title":"Full-length genome characterization of a novel mitovirus isolated from the root rot fungus Armillaria mellea","authors":"Sıla Erkmen, Ergin Sahin, Ilgaz Akata","doi":"10.1007/s11262-023-02041-8","DOIUrl":"https://doi.org/10.1007/s11262-023-02041-8","url":null,"abstract":"Members of the genus Armillaria belong to the group of pathogenic and facultative saprotrophic fungi that are generally known as one of the causative agents of white root rot in infected plants including deciduous and evergreen trees and shrubs. Although several single-stranded RNA mycoviruses were previously described in different Armillaria species, there is no report on mitoviruses (one of the simplest RNA viruses of fungal hosts) known to infect Armillaria taxa. In this study, a new mitovirus denominated “Armillaria mellea mitovirus 1” (AmMV1) was identified in the sporophore samples of Armillaria mellea, commonly known as honey mushroom. AmMV1 has a genome length of 4440 nucleotides and a G + C content of 48%. It encompasses a single open reading frame (ORF) that encodes an RNA-dependent RNA polymerase (RdRp). Comparison through BLASTp analysis revealed that the RdRp domain of AmMV1 shares a sequence identity ranging from 33.43% to 43.27% with RdRp domains of Duamitovirus genus members, having the highest similarity (43.27%) to Rhizoctonia solani mitovirus 94. According to phylogenetic analysis, AmMV1 is classified as a member of the genus Duamitovirus belonging to the Mitoviridae family. This marks the initial instance of a mitovirus identified in Armillaria spp..","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"12 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138690891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens 首次报告日本鸡禽类轮状病毒 A 的全基因组序列分析

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-15 DOI: 10.1007/s11262-023-02040-9

Ayana Okamoto, Hitoshi Takemae, Makoto Nagai, Shinichiro Hashimoto, Tetsuya Mizutani, Tetsuya Furuya

{"title":"First report of the whole-genome sequence analysis of avian rotavirus A from Japanese chickens","authors":"Ayana Okamoto, Hitoshi Takemae, Makoto Nagai, Shinichiro Hashimoto, Tetsuya Mizutani, Tetsuya Furuya","doi":"10.1007/s11262-023-02040-9","DOIUrl":"https://doi.org/10.1007/s11262-023-02040-9","url":null,"abstract":"Rotavirus A infects many mammalian species, including humans and causes diarrhea and gastrointestinal diseases. The virus also infects various bird species, including chickens, although information of avian rotavirus A (ARVA) infection in chicken populations in Japan is scarce. In this study, we report for the first time the whole-genome sequences of ARVA strains from Japanese chicken populations. The virus strains were inoculated to MA104 cells and cultured viruses were used to obtain the sequences with the MiSeq system, and genetic analysis demonstrated the genotype constellation of G19-P[30]-I11-R6-C6-M7-A16-N6-T8-E10-H8 of the Japanese chicken ARVA isolates. Phylogenetic analyses demonstrated that the VP1, VP2, VP3, VP4, VP7, NSP2, and NSP4 coding gene sequences of the Japanese strains were closer to those of Korean than the European ARVA strains, although such relationship was not clear for other genes. The data suggest that the Japanese ARVA strains and the ones in Korea have genetically close relationship, although the origin is not clear at this point. Further information including the whole-genome sequences of the Korean strains and sequences of other Japanese chicken ARVA strains will be necessary for elucidation of their origin.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"33 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138690894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aborted infection of human sodium taurocholate cotransporting polypeptide (hNTCP) expressing woodchuck hepatocytes with hepatitis B virus (HBV). 表达人牛磺胆酸钠协同转运多肽（hNTCP）的土拨鼠肝细胞感染乙型肝炎病毒（HBV）中止。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-09-20 DOI: 10.1007/s11262-023-02031-w

Lu Yang, Di Zhou, Kächele Martin, Jun Wu, Mingfa Chen, Mengji Lu, Dongliang Yang, Ulrike Protzer, Michael Roggendorf, Jingjiao Song

{"title":"Aborted infection of human sodium taurocholate cotransporting polypeptide (hNTCP) expressing woodchuck hepatocytes with hepatitis B virus (HBV).","authors":"Lu Yang, Di Zhou, Kächele Martin, Jun Wu, Mingfa Chen, Mengji Lu, Dongliang Yang, Ulrike Protzer, Michael Roggendorf, Jingjiao Song","doi":"10.1007/s11262-023-02031-w","DOIUrl":"10.1007/s11262-023-02031-w","url":null,"abstract":"Due to the limited host range of HBV, research progress has been hindered by the absence of a suitable animal model. The natural history of woodchuck hepatitis virus (WHV) infection in woodchuck closely mirrors that of HBV infection in human, making this species a promising candidate for establishing both in vivo and in vitro HBV infection models. Therefore, this animal may be a valuable species to evaluate HBV vaccines and anti-HBV drugs. A significant milestone in HBV and hepatitis D virus (HDV) infection is the discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the functional receptor. In an effort to enhance susceptibility to HBV infection, we introduced hNTCP into the woodchuck hepatocytes by multiple approaches including transduction of vLentivirus-hNTCP in woodchuck hepatocytes, transfection of p-lentivirus-hNTCP-eGFP plasmids into these cells, as well as transduction of vAdenovirus-hNTCP-eGFP. Encouragingly, our findings demonstrated the successful introduction of hNTCP into woodchuck hepatocytes. However, it was observed that these hNTCP-expressing hepatocytes were only susceptible to HDV infection but not HBV. This suggests the presence of additional crucial factors mediating early-stage HBV infection that are subject to stringent species-specific restrictions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"823-830"},"PeriodicalIF":1.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequence of Xanthomonas phage M29, a new member of Foxunavirus isolated in the Czech Republic. 捷克分离的foxfoxavirus新成员黄单胞菌噬菌体M29的全基因组序列。

IF 1.6 4区医学

Virus Genes Pub Date : 2023-12-01 Epub Date: 2023-09-04 DOI: 10.1007/s11262-023-02027-6

Mária Neoralová, Sára Brázdová, Aleš Eichmeier, Karel Petrzik

引用次数: 0