Virus Genes最新文献

{"title":"Natural occurrence of a slow lytic pseudomonas phage in a Pediatric case of multidrug-resistant P. aeruginosa severe pneumonia.","authors":"Jiaying Sun, Jingru Zhou, Long Wang, Dilare Lidifu, Ping Ni, Qin Li, Xuhua Zhang, Wei Li, Wujin Chen","doi":"10.1007/s11262-026-02271-6","DOIUrl":"https://doi.org/10.1007/s11262-026-02271-6","url":null,"abstract":"<p><p>Pseudomonas aeruginosa (P. aeruginosa) is widely distributed in the environment. As an opportunistic pathogen, it commonly causes infections in immunocompromised individuals, including respiratory tract infections and burn wound infections. P. aeruginosa possesses multiple antibiotic resistance mechanisms, including efflux pumps, resistance genes, and population dynamics. Phage therapy is a potential approach for addressing drug-resistant P. aeruginosa infections; however, clinical experience and standardized guidelines for its application in severe pneumonia remain limited. A 14-month-old infant was hospitalized for pneumonia. Four days later, he developed acute pneumonia and was sent to the ICU for 38 days of antibiotic therapy; nonetheless, P. aeruginosa remained detectable in the patient's respiratory secretions. During the clinical course, phage zjk6 was detected from a longitudinal P. aeruginosa isolate in the absence of phage therapy. This finding documents the coexistence of a naturally detected phage and MDR P. aeruginosa during prolonged pneumonia, but does not establish that the phage mediated bacterial clearance or clinical recovery. We performed whole-genome sequencing on P. aeruginosa isolates from patients to ascertain if they were infected by the same infection and assessed their antibiotic resistance using drug sensitivity testing. We isolated phages using the drip technique and double-layer plate method, examined their appearance by transmission electron microscopy, and assessed their biological properties through one-step growth curve analysis and lysis spectrum detection. Genome sequencing and comparative genomic analyses were performed to characterize phage zjk6 and representative bacterial isolates and to evaluate phage-host genomic relatedness. P. aeruginosa was isolated repeatedly during 49 days of treatment. Comparative genomic analysis of representative longitudinal isolates revealed multiple strain backgrounds, including distinct ST508 and ST266 lineages and a closely related ST836 lineage. Phage zjk6 was isolated from the fifth clinical isolate, which served as the propagation/reference host. This phage possesses an elongated tail and a limited lysis spectrum, which is capable of gradually lysing the fifth isolated P. aeruginosa strain. Genomic analysis showed that zjk6 formed plaques and displayed slow lytic behavior under the tested conditions, while also carrying lysogeny-associated regulatory modules, indicating temperate potential rather than a strictly lytic lifestyle. A naturally detected slow lytic Pseudomonas phage may coexist with MDR P. aeruginosa during prolonged infection. These findings support further study of phage-bacterium interactions in clinical infections, while the therapeutic significance of zjk6 requires additional validation.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virome of neotropical Sabethes mosquitoes reveals two novel viruses in the Spiciviridae family.","authors":"Endrya do Socorro Foro Ramos, Lilian de Oliveira Guimaraes, Geovani de Oliveira Ribeiro, Marcos Anicete Santos, Ramendra Pati Pandey, Simone Luchetta Reginato, Juliana Telles-de-Deus, Eduardo Sterlino Bergo, Luis Filipe Mucci, Vera Lucia Fonseca de Camargo-Neves, Antonio Charlys da Costa, Elcio Leal, Karin Kirchgatter","doi":"10.1007/s11262-026-02269-0","DOIUrl":"https://doi.org/10.1007/s11262-026-02269-0","url":null,"abstract":"<p><p>The family Spiciviridae, belonging to the order Ghabrivirales, is currently composed of only one officially recognized genus, Spicivirus. However, metagenomic studies have revealed an increasing diversity of related viruses, suggesting that the current classification may underestimate their evolutionary complexity. In this context, the aim of the present study was to detect and characterize new viruses related to the Spiciviridae in the family Culicidae mosquitoes. A total of nineteen mosquito pools were sequenced and analyzed using metagenomic approaches and bioinformatics tools. Among these, two novel viruses were identified in four pools (S4, S11, S18, and S19), provisionally named Sabethes virus 1 (SV1) and Sabethes virus 2 (SV2). Phylogenetic inference revealed clades with strong statistical support and genetic divergences greater than 20% in the RNA-dependent RNA polymerase (RdRp) protein. These findings contribute to a detailed analysis of the genomic diversity associated with Spiciviridae. Although Spicivirus is currently the only genus officially recognized, our results suggest the presence of viruses that may represent new taxonomic groupings not yet described by the ICTV.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148867729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Transcriptome mining expands the diversity of Antonospora locustae virus 1‑like viruses in the family Amalgaviridae.","authors":"Dongjin Choi, Seungwoo Baek, Myeung Seok Choi, Yoonsoo Hahn","doi":"10.1007/s11262-026-02268-1","DOIUrl":"https://doi.org/10.1007/s11262-026-02268-1","url":null,"abstract":"","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148857855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of a rare norovirus GII.8[P8] strain detected during pediatric acute gastroenteritis surveillance in Harbin, China.","authors":"Yuan Tian, Xiqiao Du, Tuo Dong, Zhe Zhang","doi":"10.1007/s11262-026-02267-2","DOIUrl":"https://doi.org/10.1007/s11262-026-02267-2","url":null,"abstract":"<p><p>Norovirus is a major causative agent of pediatric acute gastroenteritis, whereas GII.8[P8] represents a rare, non-dominant genotype with limited publicly available whole-genome data. We characterized a GII.8[P8] norovirus strain, Harbin-Nov-076-2022, detected through routine surveillance of pediatric acute gastroenteritis in Harbin, China. The strain was obtained from a stool sample of a 15-month-old female patient and subjected to whole-genome sequencing using the DNBSEQ-T7 platform. Integrated analyses included phylogenetic reconstruction, amino acid entropy profiling, selection pressure assessment, homology modeling of the capsid P protein, and B-cell epitope prediction. The near-complete genome of Harbin-Nov-076-2022 is 7486 nt in length, with a GC content of 53.81%, and displays the canonical three-open-reading-frame organization of human noroviruses. The sequence was deposited in GenBank under accession number PV416744. Phylogenetic analysis assigned the strain to the GII.8[P8] lineage, with the highest nucleotide identity of 97.52% to the Thai 2018 strain GII/Hu/TH/2018/GII.8[P8]/B6213 (ACCN: OR546393). Amino acid variability was highest in the VP2 region, with three variable sites (residues 15, 122, and 229) identified among the aligned GII.8[P8] VP2 sequences, whereas selection pressure analysis indicated that the genome was largely constrained by purifying selection. Same-template P-domain modeling predicted localized sequence-associated variations in the P2 subdomain and HBGA-binding-associated surface loops, with 59 predicted B-cell epitopes. These findings expand whole-genome evidence for rare GII.8[P8] norovirus in Northeast China and provide regional genomic data for molecular surveillance, evolutionary assessment, and antigenic characterization of non-dominant norovirus genotypes.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148801305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated in silico screening and biophysical evaluation of three plant flavonoids interacting with Newcastle disease virus proteins.","authors":"Rashmi Singh, Sachin Kumar, Latha Rangan","doi":"10.1007/s11262-026-02266-3","DOIUrl":"https://doi.org/10.1007/s11262-026-02266-3","url":null,"abstract":"<p><p>Newcastle disease virus (NDV) represents a major threat to the worldwide poultry industry, and the absence of approved antiviral therapeutics highlights the need for alternative intervention strategies. In this study, an integrated computational and experimental methodology to assess the interaction of selected plant-derived flavonoids against NDV. Molecular docking was conducted to evaluate the interactions of karanjin, mammeigin, and 3, 5-dihydroxy-4',7-dimethoxyflavone (DHDM) with multiple NDV proteins, comprising hemagglutinin neuraminidase (HN), fusion protein (F), matrix protein (M), nucleoprotein (N), and phosphoprotein (P). Based on docking predictions, the NDV phosphoprotein was selected for experimental validation of biophysical interaction studies. Among the tested compounds, karanjin consistently exhibited the strongest binding affinities, with particularly favorable interactions observed for the phosphoprotein, a key regulator of viral transcription and replication. In silico pharmacokinetic analysis further supported the drug-likeness and oral absorption potential of karanjin. Based on docking predictions, the NDV phosphoprotein was selected for experimental validation. Recombinant phosphoprotein was expressed and purified, and flavonoid interactions were examined using UV-Vis absorption spectroscopy, fluorescence quenching, and isothermal titration calorimetry analysis. Karanjin displayed a clear and specific binding profile characterized by moderate-to-strong affinity and an enthalpy-driven interaction, whereas mammeigin and DHDM showed weak or nonspecific interactions. Spectroscopic analyses corroborated these findings, indicating stable complex formation without major structural perturbation. Collectively, these results identify karanjin as a promising flavonoid candidate targeting the NDV phosphoprotein.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148708500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Municipal waste workers and hepatitis B and C virus and HTLV infection a cross-sectional study.","authors":"Fatemeh Rafiei-Abatari, Zahra Mohtasham-Amiri, Arash Pourgholaminejad, Reza Jafari-Shakib","doi":"10.1007/s11262-026-02247-6","DOIUrl":"10.1007/s11262-026-02247-6","url":null,"abstract":"<p><p>Globally, millions of individuals are infected with blood-borne pathogens such as hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-lymphotropic virus (HTLV). Municipal waste workers (MWWs) are recognized as vulnerable populations for acquiring these infections due to exposure to contaminated waste. Therefore, this study was designed to assess the seroprevalence of HBV, HCV, and HTLV infections among MWWs in Rasht, the most populous city in Guilan province in Northern Iran. In this cross-sectional study conducted in 2023, blood samples were collected from MWWs. Following serum separation, serological testing for hepatitis B surface antigen (HBsAg), anti-HCV antibodies, and anti-HTLV-1/2 antibodies was performed using enzyme-linked immunosorbent assay (ELISA) techniques. Comprehensive demographic, behavioral, and medical history data were obtained through structured interviews, and data were analyzed using SPSS software version 21. Among 511 male workers (from 24 to 60 years old), 48 individuals (9.4%) reported no HBV vaccination, 70.4% had experienced injury by a sharp object in the waste, and 35.8% of them reported needle-stick injuries. Positive tests for anti-HCV antibodies, HBsAg, and anti-HTLV-1/2 were 3 (0.59%), 1 (0.2%), and 0 (0%), respectively. The seroprevalence of HCV observed in this study was marginally higher than that reported in the general population of Guilan province. Considering the high rate of occupational injuries among MWWs, it is imperative to implement comprehensive occupational health strategies such as routine screening, safety training programs to minimize exposure risks, and vaccination programs (for HBV vaccine if unvaccinated) for waste workers upon employment.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"498-504"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148152048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of the Chikungunya virus E2 gene may have implications for the design of vaccines and therapeutic antibodies.","authors":"Nouh Saad Mohamed, Ayman Ahmed","doi":"10.1007/s11262-026-02246-7","DOIUrl":"10.1007/s11262-026-02246-7","url":null,"abstract":"<p><p>Chikungunya virus (CHIKV) is an arthropod-borne Alphavirus primarily transmitted by Aedes mosquitoes, causing sudden outbreaks with high morbidity and chronic polyarthritis. Following the US FDA approval of the first CHIKV vaccine in November 2023, concerns remain regarding vaccine efficacy due to the existence of at least three major CHIKV lineages. We conducted an in-silico genomic analysis of the CHIKV E2 glycoprotein to evaluate genetic diversity and evolutionary patterns relevant to vaccine and therapeutic antibody-binding effectiveness. The E2 gene exhibited substantial variation, reflecting high polymorphism and both intra- and inter-lineage differences. The variations detected may reduce antibody-antigen binding affinity, potentially compromising vaccine and therapeutic antibody efficacy. Our findings underscore the importance of incorporating pathogen genetic diversity into vaccine and therapeutic design. Long-term, pan-CHIKV vaccines could offer sustainable, cost-effective protection. Validation through in vitro and in vivo studies, alongside strengthened vector surveillance and control, is recommended to mitigate ongoing outbreaks.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"470-483"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148158686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a cytokine-associated nomogram for predicting severe influenza A virus infection in children.","authors":"Junxiang Li, Yuxia Yang, Ziwei Yue, Ruijie Zhang","doi":"10.1007/s11262-026-02237-8","DOIUrl":"10.1007/s11262-026-02237-8","url":null,"abstract":"<p><p>To investigate the risk factors for severe influenza A virus (IAV) infection in children and construct a nomogram prediction model based on these factors. A retrospective analysis was conducted on the clinical data of 178 children with IAV infection admitted to the Third Affiliated Hospital of Zhengzhou University between January and February 2025. According to disease severity, patients were divided into a mild group (n = 123) and a severe group (n = 55). The severe group was further stratified into a pneumonia subgroup (n = 25) and a non-pneumonia subgroup (n = 30) based on the presence of pneumonia. General clinical characteristics were compared between the mild and severe groups. Variables identified through univariate analysis were entered into multivariate logistic regression to determine independent risk factors for severe IAV infection. A nomogram model was subsequently established, and its performance was evaluated and validated using calibration curves, receiver operating characteristic (ROC) curves, and decision curve analysis (DCA). Compared with the mild group, children in the severe group were older, had longer hospital stays and prolonged fever duration, and had higher incidences of pneumonia and mental status abnormalities (all P < 0.05). Regarding immune inflammatory indicators, neutrophil percentage, Interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), IL-5, IL-12P70, and immunoglobulin G (IgG) and IgA levels were significantly higher in the severe group than in the mild group (all P < 0.05), whereas Interferon-alpha (IFN-α) levels were significantly lower (P < 0.05). Multivariate logistic regression analysis demonstrated that elevated IL-2 and TNF-α levels, prolonged fever duration, and mental status abnormalities were independently associated with severe IAV infection (all P < 0.05). The calibration curve, ROC curve, and DCA indicated that the nomogram model had good predictive Value and clinical utility. The multidimensional nomogram constructed by integrating IL-2, TNF-α, and fever duration and mental status abnormalities demonstrates high predictive value and clinical utility in forecasting severe IAV infection. This model aids in the early identification of severe IAV infections, thereby reducing the occurrence of long-term complications and enhancing the accuracy and timeliness of clinical decision-making.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"446-457"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147989221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation-induced cytidine deaminase (AID) suppresses the activity of HBV EnhII/CP by downregulating FANCE expression.","authors":"Weiping Zhou, Biao Yang, Ye Sun, Yuxin Bai, Xuanhe Fu, Bing Nie, Ying Li, Xing Tian, Zhongjia Jiang, Guangyan Liu","doi":"10.1007/s11262-026-02244-9","DOIUrl":"10.1007/s11262-026-02244-9","url":null,"abstract":"<p><p>Activation-induced cytidine deaminase (AID) is a host restriction factor known to suppress hepatitis B virus (HBV) replication. However, the precise mechanisms underlying its antiviral activity remain incompletely understood. Combining RNA-Seq and functional assays, our study reveals a novel pathway by which AID inhibits HBV replication via downregulating the expression of Fanconi anemia complementation group E (FANCE). Our findings demonstrate that AID significantly reduces FANCE expression, and that FANCE promotes viral replication by specifically activating the HBV enhancer II/core promoter (EnhII/CP), a key transcriptional regulatory element. Combinatorial regulation experiments further confirm that FANCE is involved in AID-mediated HBV transcription. These results identify a novel downstream gene of AID, broaden the perspective on the host antiviral immune network against HBV, and provides a rationale for potential antiviral strategies targeting the FANCE regulatory axis.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"458-469"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of flaviviruses in mosquitoes from a livestock farm in Ibadan, Nigeria.","authors":"Oluwapelumi S Ogunlusi, Adewale V Opayele, Babatunde O Motayo, Adedayo O Faneye","doi":"10.1007/s11262-026-02251-w","DOIUrl":"10.1007/s11262-026-02251-w","url":null,"abstract":"<p><p>Orthoflaviviruses constitute a diverse genus within the Flaviviridae family, comprising over 70 enveloped, single-stranded positive-sense RNA viruses that pose significant global health threats. Yellow fever virus (YFV) remains a significant public health concern in Nigeria, with suboptimal vaccination coverage and a persistent risk of transmission. Understanding the circulation of local orthoflaviviruses in mosquito vectors is crucial for disease surveillance and prevention strategies. Adult female mosquitoes were collected from the University of Ibadan's dairy and teaching farms between June and August 2022 using stationary human-bait catches and miniature light traps. Mosquitoes were morphologically identified, pooled by species and collection site, and screened for orthoflaviviruses using hemi-nested reverse transcription PCR targeting the NS5 gene. Positive samples were sequenced and phylogenetic analysis was carried out. A total of 600 mosquitoes, representing six species from three genera, were collected, with Aedes aegypti predominating (60.67%). Of 22 mosquito pools tested, three were positive for orthoflaviviruses. Yellow fever virus was detected in one pool of 37 A. aegypti specimens, whilst insect-specific flaviviruses were identified in two other pools of the same mosquito genus. Phylogenetic analysis placed the YFV isolate within the West African genotype, consistent with genotypic affiliation to West African reference strains; however, fine-scale clustering inferences are limited by the short (~ 220 bp) NS5 fragment used. This study provides molecular evidence of orthoflavivirus nucleic acid in mosquito populations at a peri-agricultural site in Ibadan, Nigeria. The detection of YFV RNA in a single A. aegypti pool, at a minimum infection rate of 5.49 per 1000 specimens (95% CI: 1.34-19.95), is consistent with low-level vector-associated viral presence and warrants continued entomological and virological surveillance. These findings do not independently establish active transmission cycles or quantifiable human risk, but highlight the need for integrated virological and serological monitoring at human-animal-environment interfaces.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"484-497"},"PeriodicalIF":2.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148229162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}