Virus Genes最新文献

{"title":"Pseudorabies virus as a zoonosis: scientific and public health implications.","authors":"Yumei Chen, Jie Gao, Rongqian Hua, Gaiping Zhang","doi":"10.1007/s11262-024-02122-2","DOIUrl":"https://doi.org/10.1007/s11262-024-02122-2","url":null,"abstract":"<p><p>Pseudorabies virus (PRV) is a herpes virus, also known as Aujeszky's disease virus (ADV), which can cause a highly infectious disease pseudorabies (PR) in a variety of mammals. In the past, it has been debated whether PRV can infect humans, but more and more cases of PRV infection have been reported since 2017. The illness has claimed many victims and left survivors with serious sequelae. This indicates that humans may ignore the zoonotic ability of PRV. This review aims to summarize the pathology and pathogenesis of PRV and speculate on how it infects humans. This paper provides a comprehensive overview of the progression of PRV, including its virology characteristics, genomic organization, and genetic evolution. It also synthesises the existing literature on PRV infection in humans, and analyses the factors contributing to PRV zoonosis. Finally, the pathogenesis of PRV-infected pigs and other mammals was summarized, and the pathogenesis of PRV-infected humans was speculated.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of thymidine kinase-deficiency (∆ORF38) on neuropathogenicity of equine herpesvirus-1 in the mouse model and expression of neighboring genes.","authors":"Samy Kasem, Ahmed S Abdel-Moneim, Hideto Fukushi","doi":"10.1007/s11262-024-02128-w","DOIUrl":"https://doi.org/10.1007/s11262-024-02128-w","url":null,"abstract":"<p><p>Previous studies showed that deletion of the viral thymidine kinase (TK) gene in several alphaherpesviruses including EHV-1 reduced their virulence. Previously, we found that deletion of ORF37, which is located head-to-head with TK, decreased EHV-1 virulence in mice but did not affect the expression of TK mRNA. Therefore, deletion of ORF38 might also affect virulence by partially deleting the ORF37 promoter. To investigate the role of the TK gene-encoding region in the pathogenesis of EHV-1 as well as the expression of ORF37, we generated a TK deletion mutant by using a bacterial artificial chromosome carrying the neuropathogenic strain Ab4p. Deletion of TK increased the transcription of ORF37, did not cause any neurological disorders in CBA/N1 mice, and its growth in cultured neural cells was impaired. These results suggest deletion of ORF38 does not affect the ORF37 promoter and confirm that TK plays an important role in the neuropathogenicity of EHV-1.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization of a novel HIV-1 circulating recombinant form (CRF162_cpx) involving CRF01_AE, CRF07_BC and subtype B in Guangdong, China.","authors":"Yun Lan, Linghua Li, Mingfeng Xiao, Yaqing Lin, Xuemei Ling, Feng Li, Fengyu Hu","doi":"10.1007/s11262-024-02127-x","DOIUrl":"https://doi.org/10.1007/s11262-024-02127-x","url":null,"abstract":"<p><p>Human immunodeficiency virus type 1 (HIV-1) is characterized by its extremely high level of genetic diversity. The spread of different subtypes in the same population often leads to the emergence of circulating recombinant forms (CRFs) and unique recombinant forms (URFs). At present, the main recombinant subtypes of HIV-1 in China originate from CRF07_BC, CRF01_AE, CRF55_01B and subtype B. Here, we obtained the nearly full-length genomes (NFLGs) from eight HIV-1 infected patients in Guangdong Province, which shared highly similar recombinant patterns, involving two CRF01_AE, one CRF07_BC and two subtype B segments. The eight NFLG sequences own four similar breakpoints as follows: 1220 nucleotide (nt), 2243 nt, 2673 nt, and 5820 nt according to the HXB2 reference sequence, and they therefore were assigned as CRF162_cpx. This is the first complex CRF derived from CRF01_AE, CRF07_BC and subtype B in China. The Bayesian inference of the segments showed that HIV-1 CRF162_cpx was inferred to have approximately originated around 2010-2015. The emergence of CRF162_cpx indicates that the HIV diversity in southeast China constantly accumulates and evolves. Thus, intensive surveillance of HIV-1 molecular epidemiology should be reinforced.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appelmans Protocol for in vitro Klebsiella pneumoniae phage host range expansion leads to induction of the novel temperate linear plasmid prophage vB_KpnS-KpLi5.","authors":"Nadine Jakob, Jens A Hammerl, Brett E Swierczewski, Silvia Würstle, Joachim J Bugert","doi":"10.1007/s11262-024-02124-0","DOIUrl":"https://doi.org/10.1007/s11262-024-02124-0","url":null,"abstract":"<p><p>Adjuvant therapy with bacteriophage (phage) cocktails in combination with antibiotics is a therapeutic approach currently considered for treatment of infections with encapsulated, biofilm forming, and multidrug-resistant Klebsiella pneumoniae (Kp). Klebsiella phage are highly selective in targeting a bacterial capsule type. Considering the numerous Kp capsule types and other host restriction factors, phage treatment could be facilitated when generating phages with a broad host range. A modified 'Appelmans protocol' was used to create phages with an extended host range via in vitro forced DNA recombination. Three T7-like Kp phages with highly colinear genomes were subjected to successive propagation on their susceptible host strains representing the capsule types K64, K27, and K23, and five Kp isolates of the same capsule types initially unsusceptible for phage lysis. After 30 propagation cycles, five phages were isolated via plaque assay. Four output phages represented the original input phages, while the fifth lysed a previously non-permissible Kp isolate, which was not lysed by any of the input phages. Surprisingly, sequence analysis revealed a novel N15/phiKO2-like phage genome (vB_KpnS_KpLi5) lacking substantial homologies to any of the used T7-like phages. This phage is not a chimeric recombinant of the applied T7-like phages, but represents a temperate phage that was induced from Kp due to the application of the input phages phages (cocktail), but not by any of them individually. Adapted phages with chimeric genomes and extended host range derived from input phages were not observed. Induction of temperate phages may be a stress response caused by using multiple phages simultaneously (i.e., by destabilization of the cell wall due to an unspecific binding of the phages). Successive use of different phages for therapeutic purposes may be preferable over simultaneous application in cocktail formulations to avoid undesired induction of temperate phages.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complete genomic sequence of Magnaporthe oryzae polymycovirus 1.","authors":"Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng","doi":"10.1007/s11262-024-02126-y","DOIUrl":"https://doi.org/10.1007/s11262-024-02126-y","url":null,"abstract":"<p><p>A novel double-stranded RNA virus, designated as \"Magnaporthe oryzae polymycovirus 1\" (MoPmV1), was identified in Magnaporthe oryzae strain TM02. MoPmV1 has four dsRNA fragments, ranging from 1324 to 2401 bp in length. DsRNA1, 2, and 3 of MoPmV1 each possess a single large open reading frame (ORF), whereas dsRNA4 contains two ORFs. BLASTp analysis indicated that ORF1a, encoded by dsRNA1, shows 59.2% amino acid sequence identity with the RNA-dependent RNA polymerase (RdRp) of Beauveria bassiana polymycovirus 2; ORF2a, encoded by dsRNA2, shows 42.3% identity with the putative serine protease of Phaeoacremonium minimum tetramycovirus 1; ORF3a, encoded by dsRNA3, shows 40.6% identity with the methyltransferase of Aspergillus fumigatus tetramycovirus 1; ORF4a, encoded by dsRNA4, shows 41.7% identity with the proline-alanine-serine-rich (PASr) protein of Botryosphaeria dothidea virus 1, while ORF4b, encoded by dsRNA4, shows no significant similarity to any known proteins. Phylogenetic analysis of the RdRp domain indicated that MoPmV1 was grouped in a cluster with members of the genus Polymycovirus in the family Polymycoviridae. Based on these characteristics, MoPmV1 is a new member of the genus Polymycovirus in the family Polymycoviridae. This is the first report of a mycovirus of the family Polymycoviridae identified in rice blast fungus M. oryzae.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and molecular epidemiology of canine parvovirus and identification of highly pathogenic CPV-2c isolates from Shandong, China.","authors":"Jiahui Li, Baoyu Cheng, Zihe Li, Yanlei Cui, Haiyan Yang, Weiquan Liu, Chuanmei Zhang, Yongle Yu","doi":"10.1007/s11262-024-02125-z","DOIUrl":"https://doi.org/10.1007/s11262-024-02125-z","url":null,"abstract":"<p><p>Canine parvovirus (CPV) is an important pathogen of dogs and wild carnivores. It is a single-stranded DNA virus with a high mutation frequency and antigenic drift. To research the prevalence and genetic variation of CPV in Shandong, 62 samples from diseased dogs were collected and examined by using PCR for parvovirus. Our results showed that the positivity was 62.9% (n = 39), VP2 gene were sequenced and compared with reference strains. For the parvovirus subtype prevalence, 7 strains were CPV-2a (17.9%) and 32 strains were CPV-2c (82.1%). The results of phylogenetic analysis of VP2 gene of the CPVs showed all 39 isolates formed a major clade and were distantly related to the commercial vaccine strains. By comparing amino acid (aa) sequences, this study discovered new mutations not previously reported which may be related to host range and antigenicity. Moreover, one CPV-2c strain (QN-55) was isolated and cultured on F81 cells, and characterized by whole-genome sequencing. The TCID₅₀ of this strain was 10^-3.2/0.1 mL and animal tests have shown that the strain is fatal to infected dogs.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic identification of monkeypox virus phylogenetic gene trees that are representative of its whole-genome phylogenetic tree.","authors":"El-Miracle Idorenyin Akpan, Diana Diaz-Cánova, Malachy Ifeanyi Okeke","doi":"10.1007/s11262-024-02110-6","DOIUrl":"10.1007/s11262-024-02110-6","url":null,"abstract":"<p><p>Phylogenetic analysis based on whole-genome sequences is the gold standard for monkeypox virus (MPXV) phylogeny. However, genomic epidemiology capability and capacity are lacking or limited in resource poor countries of sub-Saharan Africa. Therefore, these make real-time genome surveillance of MPXV virtually impossible. We hypothesized that phylogenetic analysis based on single, conserved genes will produce phylogenetic tree topology consistent with MPXV whole-genome phylogeny, thus serving as a reliable proxy to phylogenomic analysis. In this study, we analyzed 62 conserved MPXV genes and showed that Bayesian phylogenetic analysis based on five genes (OPG 066/E4L, OPG068/E6R, OPG079/I3L, OPG145/A18R, and OPG150/A23R) generated phylogenetic trees with 72.2-96.3% topology similarity index to the reference phylogenomic tree topology. Our results showed that phylogenetic analysis of the identified five genes singly or in combination can serve as surrogate for whole-genome phylogenetic analysis, and thus obviates the need for whole-genome sequencing and phylogenomic analysis in regions where genomic epidemiology competence and capacity are lacking or unavailable. This study is relevant to evolution and genome surveillance of MPXV in resource limited countries.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"635-641"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tackling hepatitis B Virus with CRISPR/Cas9: advances, challenges, and delivery strategies.","authors":"Dakshina M Nair, Leela Kakithakara Vajravelu, Jayaprakash Thulukanam, Vishnupriya Paneerselvam, Poornima Baskar Vimala, Rahul Harikumar Lathakumari","doi":"10.1007/s11262-024-02105-3","DOIUrl":"10.1007/s11262-024-02105-3","url":null,"abstract":"<p><p>Hepatitis B virus (HBV) infection remains a significant global health challenge, with chronic HBV leading to severe liver diseases, including cirrhosis and hepatocellular carcinoma. Current treatments often fail to eradicate the virus, highlighting the need for innovative therapeutic strategies. The CRISPR/Cas9 system has emerged as a dynamic tool for precise genome editing and presents a promising approach to targeting and eliminating HBV infection. This review provides a comprehensive overview of the advances, challenges, and delivery strategies associated with CRISPR/Cas9-based therapies for HBV. We begin by elucidating the mechanism of the CRISPR/Cas9 system and then explore HBV pathogenesis, focusing on the role of covalently closed circular DNA (cccDNA) and integrated HBV DNA in maintaining chronic infection. CRISPR/Cas9 can disrupt these key viral reservoirs, which are critical for persistent HBV replication and associated liver damage. The application of CRISPR/Cas9 in HBV treatment faces significant challenges, such as off-target effects, delivery efficiency, and immune responses. These challenges are addressed by examining current approaches to enhance the specificity, safety, and efficacy of CRISPR/Cas9. A future perspective on the development and clinical translation of CRISPR/Cas9 therapies for HBV is provided, emphasizing the requirement for further research to improve delivery methods and ensure durable safety and effectiveness. This review underscores the transformative potential of CRISPR/Cas9 in combating HBV and sets the stage for future breakthroughs in the field.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"592-602"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the study of LNPs for mRNA delivery and clinical applications.","authors":"Bili Wang, Biao Shen, Wenqing Xiang, Hongqiang Shen","doi":"10.1007/s11262-024-02102-6","DOIUrl":"10.1007/s11262-024-02102-6","url":null,"abstract":"<p><p>Messenger ribonucleic acid (mRNA) was discovered in 1961 as an intermediary for transferring genetic information from DNA to ribosomes for protein synthesis. The COVID-19 pandemic brought worldwide attention to mRNA vaccines. The emergency use authorization of two COVID-19 mRNA vaccines, BNT162b2 and mRNA-1273, were major achievements in the history of vaccine development. Lipid nanoparticles (LNPs), one of the most superior non-viral delivery vectors available, have made many exciting advances in clinical translation as part of the COVID-19 vaccine and therefore has the potential to accelerate the clinical translation of many gene drugs. In addition, due to these small size, biocompatibility and excellent biodegradability, LNPs can efficiently deliver nucleic acids into cells, which is particularly important for current mRNA therapeutic regimens. LNPs are composed cationic or pH-dependent ionizable lipid bilayer, polyethylene glycol (PEG), phospholipids, and cholesterol, represents an advanced system for the delivery of mRNA vaccines. Furthermore, optimization of these four components constituting the LNPs have demonstrated enhanced vaccine efficacy and diminished adverse effects. The incorporation of biodegradable lipids enhance the biocompatibility of LNPs, thereby improving its potential as an efficacious therapeutic approach for a wide range of challenging and intricate diseases, encompassing infectious diseases, liver disorders, cancer, cardiovascular diseases, cerebrovascular conditions, among others. Consequently, this review aims to furnish the scientific community with the most up-to-date information regarding mRNA vaccines and LNP delivery systems.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"577-591"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of HDAC9-induced deacetylation of glycolysis-related GAPDH lysine 219 on rotavirus replication in rotavirus-infected Caco-2 cells.","authors":"Lijun Song, Peicheng Zhong, Runyu Yu, Yue Yuan, Yujing Zhou, Yupei Qian, Siyan Yang, Haosen Yi, Zhiyan Yang, Wenchang Zhao","doi":"10.1007/s11262-024-02104-4","DOIUrl":"10.1007/s11262-024-02104-4","url":null,"abstract":"<p><p>Post-translational modifications (PTMs), as epigenetic modifications, are significant in the interaction between virus and its host. However, it is unclear whether rotavirus (RV) causes changes in both the host cell epigenetic protein modification and the regulatory mechanism of viral replication. Here, we analyzed the proteome of Caco-2 cells to determine if acetylation modification occurred within the cells after RV infection. We found that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a protein involved in glycolysis, was deacetylated at lysine 219 via histone deacetylase 9 (HDAC9) in 50 h after the RV infection. Remarkably, the deacetylation of GAPDH promoted RV replication. Finally, we found that glycolysis was alterable in Caco-2 cells by RV or the deacetylation of GAPDH lysine 219, using the Seahorse XF Glycolysis Stress Test. In conclusion, our results demonstrate for the first time that RV infection promoted deacetylation of GAPDH at lysine 219 in order to increase its own viral replication in Caco-2 cells.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"621-634"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}