Lipi Akter, Md Abul Hashem, Mohammad Enamul Hoque Kayesh, Tofazzal Md Rakib, Md Haroon Or Rashid, Fumie Maetani, Kyoko-Tsukiyama Kohara
{"title":"Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study.","authors":"Lipi Akter, Md Abul Hashem, Mohammad Enamul Hoque Kayesh, Tofazzal Md Rakib, Md Haroon Or Rashid, Fumie Maetani, Kyoko-Tsukiyama Kohara","doi":"10.1007/s11262-025-02169-9","DOIUrl":null,"url":null,"abstract":"<p><p>Koala retrovirus (KoRV) causes multiple disease phenotypes in koalas, including carcinogenesis. The study aimed to assess oncogene expression in spleen tissues from ten deceased koalas coinfected with different subtypes and peripheral blood mononuclear cells (PBMCs) from two subclinically coinfected koalas with KoRV-A and KoRV-B. Initially, KoRV subtyping involved amplifying endogenous KoRV-A, and exogenous KoRV-B, -C specific env gene fragments, followed by sequencing. Using quantitative real-time polymerase chain reaction (RT-qPCR), we examined five oncogenes (BCL2, BAX, BCL2L1, BCL3, and MYC) in spleen and PBMCs from dead and alive koalas coinfected with multiple KoRV subtypes, respectively. Significant (p < 0.05) increases in BCL2 and BAX oncogene expression were observed in deceased koalas that were coinfected with multiple KoRV subtypes compared with healthy koalas. Thus, this study highlights a potential link between KoRV subtype infections, oncogene expression, and koala diseases.</p>","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virus Genes","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11262-025-02169-9","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Koala retrovirus (KoRV) causes multiple disease phenotypes in koalas, including carcinogenesis. The study aimed to assess oncogene expression in spleen tissues from ten deceased koalas coinfected with different subtypes and peripheral blood mononuclear cells (PBMCs) from two subclinically coinfected koalas with KoRV-A and KoRV-B. Initially, KoRV subtyping involved amplifying endogenous KoRV-A, and exogenous KoRV-B, -C specific env gene fragments, followed by sequencing. Using quantitative real-time polymerase chain reaction (RT-qPCR), we examined five oncogenes (BCL2, BAX, BCL2L1, BCL3, and MYC) in spleen and PBMCs from dead and alive koalas coinfected with multiple KoRV subtypes, respectively. Significant (p < 0.05) increases in BCL2 and BAX oncogene expression were observed in deceased koalas that were coinfected with multiple KoRV subtypes compared with healthy koalas. Thus, this study highlights a potential link between KoRV subtype infections, oncogene expression, and koala diseases.
期刊介绍：
Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools.
Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments.
Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
