The complete genome sequence of a gammabaculovirus from the Virginia pine sawfly, Neodiprion pratti pratti.

IF 1.9 4区医学 Q3 GENETICS & HEREDITY

Virus Genes Pub Date : 2025-10-06 DOI:10.1007/s11262-025-02189-5

Robert L Harrison, Daniel L Rowley

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Abstract

The complete genome sequence of a baculovirus isolated from larvae of the Virginia pine sawfly, Neodiprion pratti pratti, was determined from sequence data generated from two isolates of this virus obtained from virus-killed larvae harvested during a N. pratti pratti outbreak in Maryland, Virginia, and North Carolina, USA, during the 1950s. Sequence assembly and analysis of this virus, Neodiprion pratti pratti nucleopolyhedrovirus (NeppNPV), revealed a circular genome of 81,658 bp. BLASTp queries with the 89 ORFs annotated for NeppNPV indicated a close relationship with Neodiprion lecontei nucleopolyhedrovirus (NeleNPV). Pairwise nucleotide distances and phylogeny determined from alignments of baculovirus core gene homologs indicated that NeppNPV and NeleNPV are both members of species Gammabaculovirus nelecontei. NeppNPV and NeleNPV were distinguishable by differences in ORF content and indels suggestive of intramolecular recombination. Overlapping geographic ranges and shared host plants for N. pratti pratti and N. lecontei suggest the potential for cross-infections of larvae of these two sawfly species with NeppNPV and NeleNPV.

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来自弗吉尼亚松锯蝇的一种γ -马孔病毒的全基因组序列。

从弗吉尼亚松锯蝇（nediprion pratti pratti）幼虫中分离出的杆状病毒的全基因组序列是根据20世纪50年代在美国马里兰州、弗吉尼亚州和北卡罗来纳州发生的一次N. pratti pratti暴发期间收获的病毒杀死的幼虫中分离出的两株该病毒的序列数据确定的。pratti pratti Neodiprion pratti pratti核多角体病毒（NeppNPV）的序列组装和分析显示其环状基因组长度为81,658 bp。对NeppNPV注释的89个orf进行BLASTp查询，结果表明该病毒与nediprion lecontei核多角体病毒（nenediprion lecontei nuclear polyhedrovirus, NeleNPV）关系密切。根据杆状病毒核心基因同源物比对确定的成对核苷酸距离和系统发育表明，NeppNPV和NeleNPV都是gamabaclovirus nelecontei种的成员。NeppNPV和NeleNPV通过ORF含量和提示分子内重组的索引的差异来区分。pratti N. pratti和N. lecontei具有重叠的地理分布范围和共同的寄主植物，这表明这两种锯蝇的幼虫可能与NeppNPV和NeleNPV交叉感染。

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来源期刊

Virus Genes 医学-病毒学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.