Virus Genes最新文献_第4页

Long non-coding RNA C1RL-AS1 aggravates influenza A virus pneumonia through miR-16-5p/LAMP3. 长链非编码RNA C1RL-AS1通过miR-16-5p/LAMP3加重甲型流感病毒肺炎

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-01-02 DOI: 10.1007/s11262-024-02131-1

Xingjuan Liao, Qin Liang, Chao Xu, Xinbing Luo

{"title":"Long non-coding RNA C1RL-AS1 aggravates influenza A virus pneumonia through miR-16-5p/LAMP3.","authors":"Xingjuan Liao, Qin Liang, Chao Xu, Xinbing Luo","doi":"10.1007/s11262-024-02131-1","DOIUrl":"10.1007/s11262-024-02131-1","url":null,"abstract":"Influenza A viruses continue to pose a serious threat to public health and economic stability. To investigate the role of C1RL-AS1 in influenza A virus (IAV) pneumonia. Using RT-qPCR analysis, we determined C1RL-AS1 expression levels in children with IAV-infected pneumonia and A549 cells. C1RL-AS1 expression levels in children were subjected to ROC analysis. C1RL-AS1 was knocked down to investigate its role in IAV-infected A549 cells, including effects on viral nucleoprotein (NP) production, cell survival, and apoptosis. Downstream miRNAs of C1RL-AS1 were predicted and validated. MiR-16-5p target genes were predicted and validated. C1RL-AS1 was up-regulated in IAV-infected children and A549 cells. C1RL-AS1 expression levels distinguished children with IAV pneumonia from healthy children. Knockdown of C1RL-AS1 attenuated viral NP production, promoted A549 cell survival, and inhibited apoptosis. MiR-16-5p was a downstream C1RL-AS1 miRNA. miR-16-5p counteracted the anti-IAV infection effect brought about by C1RL-AS1 knockdown. LAMP3 was a miR-16-5p target gene associated with pneumonia. LAMP3 restored the cellular effects brought about by C1RL-AS1/miR-16-5p co-knockdown. C1RL-AS1 is a possible diagnostic factor for IAV pneumonia in children. C1RL-AS1 may participate in IAV pneumonia by sponging miR-16-5p and then moderating LAMP3.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"145-152"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete genomic sequence of Magnaporthe oryzae polymycovirus 1. 米大孔菌多分枝病毒1型全基因组序列。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-06 DOI: 10.1007/s11262-024-02126-y

Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng

{"title":"The complete genomic sequence of Magnaporthe oryzae polymycovirus 1.","authors":"Hong Zheng, Cong Li, Yuxin Wu, Xinyi Li, Hongliu An, Shouguo Fang, Songbai Zhang, Qingchao Deng","doi":"10.1007/s11262-024-02126-y","DOIUrl":"10.1007/s11262-024-02126-y","url":null,"abstract":"A novel double-stranded RNA virus, designated as \"Magnaporthe oryzae polymycovirus 1\" (MoPmV1), was identified in Magnaporthe oryzae strain TM02. MoPmV1 has four dsRNA fragments, ranging from 1324 to 2401 bp in length. DsRNA1, 2, and 3 of MoPmV1 each possess a single large open reading frame (ORF), whereas dsRNA4 contains two ORFs. BLASTp analysis indicated that ORF1a, encoded by dsRNA1, shows 59.2% amino acid sequence identity with the RNA-dependent RNA polymerase (RdRp) of Beauveria bassiana polymycovirus 2; ORF2a, encoded by dsRNA2, shows 42.3% identity with the putative serine protease of Phaeoacremonium minimum tetramycovirus 1; ORF3a, encoded by dsRNA3, shows 40.6% identity with the methyltransferase of Aspergillus fumigatus tetramycovirus 1; ORF4a, encoded by dsRNA4, shows 41.7% identity with the proline-alanine-serine-rich (PASr) protein of Botryosphaeria dothidea virus 1, while ORF4b, encoded by dsRNA4, shows no significant similarity to any known proteins. Phylogenetic analysis of the RdRp domain indicated that MoPmV1 was grouped in a cluster with members of the genus Polymycovirus in the family Polymycoviridae. Based on these characteristics, MoPmV1 is a new member of the genus Polymycovirus in the family Polymycoviridae. This is the first report of a mycovirus of the family Polymycoviridae identified in rice blast fungus M. oryzae.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"204-210"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First detection and diversity of astroviruses in wild migratory birds of Sakhalin Island, North Pacific. 北太平洋库页岛野生候鸟中星状病毒的首次检测及其多样性。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-27 DOI: 10.1007/s11262-024-02130-2

Dmitry Zhirov, Nikita Dubovitskiy, Anastasiya Derko, Arina Loginova, Ivan Sobolev, Pavel Ktitorov, Olga Kulikova, Guimei He, Zhenghuan Wang, Wen Wang, Alexander Alekseev, Alexander Shestopalov, Kirill Sharshov

{"title":"First detection and diversity of astroviruses in wild migratory birds of Sakhalin Island, North Pacific.","authors":"Dmitry Zhirov, Nikita Dubovitskiy, Anastasiya Derko, Arina Loginova, Ivan Sobolev, Pavel Ktitorov, Olga Kulikova, Guimei He, Zhenghuan Wang, Wen Wang, Alexander Alekseev, Alexander Shestopalov, Kirill Sharshov","doi":"10.1007/s11262-024-02130-2","DOIUrl":"10.1007/s11262-024-02130-2","url":null,"abstract":"Researchers have identified Avastrovirus as a significant genus of bird viruses, linked to various avian diseases such as enteritis, growth retardation, nephritis and hepatitis. These infections can cause substantial economic losses in agrocultureand have a widespread impact on global food production. Although there have been numerous studies on these viruses, most of them-mainly focuses on poultry. Research on astroviruses in wild bird populations has revealed a wide genetic diversity of these viruses, yet our understanding of their biological and ecological characteristics remains limited. In this study, we for the first time detected avastrovirus in wild migratory birds of the families Anatidae and Columbidae from Sakhalin Island, North Pacific Ocean. Phylogenetic analysis revealed the presence of Avastrovirus 2 in wild doves and Avastrovirus 3 in wild ducks. These findings provide valuable insights into the circulation of astroviruses in wild bird populations of Sakhalin Island, which lies along the East Asian-Australasian Flyway.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"244-248"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of thymidine kinase-deficiency (∆ORF38) on neuropathogenicity of equine herpesvirus-1 in the mouse model and expression of neighboring genes. 胸苷激酶缺乏（∆ORF38）对马疱疹病毒-1小鼠模型神经致病性及邻近基因表达的影响

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2024-12-16 DOI: 10.1007/s11262-024-02128-w

Samy Kasem, Ahmed S Abdel-Moneim, Hideto Fukushi

引用次数: 0

Pooled screening for endogenous HHV-6 in subjects with coronary artery disease. 冠状动脉疾病患者内源性HHV-6的合并筛查

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-01-31 DOI: 10.1007/s11262-025-02134-6

Rie Koide, Yoshie Nakashima, Shohei Kojima, Yohei Sotomi, Yasushi Sakata, Yasuhiko Sakata, Kaoru Ito, Nicholas F Parrish

引用次数: 0

ALR inhibits HBV replication and autophagosome formation by ameliorating HBV-induced ROS production in hepatic cells. ALR通过改善肝细胞中HBV诱导的ROS产生来抑制HBV复制和自噬体的形成。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-04-01 Epub Date: 2025-02-11 DOI: 10.1007/s11262-025-02139-1

Amit Kumar Mishra, Md Musa Hossain, Teja Naveen Sata, Kishor Pant, Ajay K Yadav, Amrendra Kumar Sah, Parul Gupta, Md Ismail, Baibaswata Nayak, Shalimar, Senthil Kumar Venugopal

{"title":"ALR inhibits HBV replication and autophagosome formation by ameliorating HBV-induced ROS production in hepatic cells.","authors":"Amit Kumar Mishra, Md Musa Hossain, Teja Naveen Sata, Kishor Pant, Ajay K Yadav, Amrendra Kumar Sah, Parul Gupta, Md Ismail, Baibaswata Nayak, Shalimar, Senthil Kumar Venugopal","doi":"10.1007/s11262-025-02139-1","DOIUrl":"10.1007/s11262-025-02139-1","url":null,"abstract":"HBV has a small genome and thrives in the infected hepatocytes by hijacking the cellular machinery and cellular pathways. HBV induces incomplete autophagy for its replication and survival. This study showed that HBV replication induces Reactive oxygen species (ROS) production, which in turn augments the formation of autophagosomes. Augmenter of liver regeneration (ALR) is a sufhydryl oxidase and has an anti-oxidative property. We sought to determine the interplay between HBV and antioxidant protein ALR. We showed that HBV downregulated ALR expression in hepatic cells. There was increased ROS production in HBV-infected cells while ALR downregulated ROS levels and expression of NADPH oxidase NOX4. N-acetyl cysteine, a ROS scavenger, downregulated ROS level and autophagosome formation in HBV-expressing cells. ALR overexpression in HBV-expressing cells downregulated the expression of autophagy marker proteins while upregulated the expression of p-MTOR. ALR overexpression decreased the expression of HBx, HBsAg, and total HBV load. This study showed that HBx relieved ALR-mediated inhibition by upregulating the miR-181a expression in HBV-infected cells, which in turn downregulated ALR expression.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"167-178"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and epidemiological analysis of a putative novel hantavirus in Australian flying foxes. 澳大利亚飞狐中一种假定的新型汉坦病毒的鉴定和流行病学分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-11 DOI: 10.1007/s11262-024-02113-3

Craig S Smith, Darren J Underwood, Anita Gordon, Michael J Pyne, Anna Smyth, Benjamin Genge, Luke Driver, David G Mayer, Jane Oakey

{"title":"Identification and epidemiological analysis of a putative novel hantavirus in Australian flying foxes.","authors":"Craig S Smith, Darren J Underwood, Anita Gordon, Michael J Pyne, Anna Smyth, Benjamin Genge, Luke Driver, David G Mayer, Jane Oakey","doi":"10.1007/s11262-024-02113-3","DOIUrl":"10.1007/s11262-024-02113-3","url":null,"abstract":"In July 2017, an investigation into the cause of neurological signs in a black flying fox (Pteropus alecto, family Pteropodidae) identified a putative novel hantavirus (Robina virus, ROBV, order Bunyavirales, family Hantaviridae, genus Mobatvirus) in its brain. Analysis of the evolutionary relationship between other hantaviruses using maximum-likelihood, a systematic Bayesian clustering approach, and a minimum spanning tree, all suggest that ROBV is most closely related to another Mobatvirus, Quezon virus, previously identified in the lung of a Philippine frugivorous bat (Rousettus amplexicaudatus, also family Pteropodidae). Subsequently, between March 2018 and October 2023, a total of 495 bats were opportunistically screened for ROBV with an experimental qRT-PCR. The total prevalence of ROBV RNA detected in Pteropus spp. was 4.2% (95% CI 2.8-6.4%). Binomial modelling identified that there was substantial evidence supporting an increase (P = 0.033) in the detection of ROBV RNA in bats in 2019 and 2020 suggesting of a possible transient epidemic. There was also moderate evidence to support the effect of season (P = 0.064), with peak detection in the cooler seasons, autumn, and winter, possibly driven by physiological and ecological factors similar to those already identified for other bat-borne viruses. This is Australia's first reported putative hantavirus and its identification could expand the southern known range of hantaviruses in Australasia.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"71-80"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142401901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The isolation and serotyping of foot-and-mouth disease virus in Iran during 2019-2022. 2019-2022 年伊朗口蹄疫病毒的分离和血清分型。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-12 DOI: 10.1007/s11262-024-02116-0

Siamak Khoshnood, Seyed Mahmoud Azimi, Zahra Ziafati Kafi, Hamideh Najafi, Arash Ghalyanchilangeroudi

{"title":"The isolation and serotyping of foot-and-mouth disease virus in Iran during 2019-2022.","authors":"Siamak Khoshnood, Seyed Mahmoud Azimi, Zahra Ziafati Kafi, Hamideh Najafi, Arash Ghalyanchilangeroudi","doi":"10.1007/s11262-024-02116-0","DOIUrl":"10.1007/s11262-024-02116-0","url":null,"abstract":"Foot-and-mouth disease (FMD) is a significant transboundary animal disease that has a considerable economic impact on livestock systems worldwide. In order to determine the presence and type of FMD virus in Iran, a total of 90 samples of vesicular fluid and epithelial tissue were collected from the tongues, tooth pads, and hooves of clinically suspect cattle on 40 vaccinated farms in 9 provinces of Iran. These samples were collected during four years, from January 2019 to December 2022, and the vaccine was a locally produced polyvalent inactivated vaccine. The collected samples were analyzed using ELISA and isolation methods to identify and characterize the FMD virus. The results of the ELISA tests revealed that 66.66% of the samples were positive for FMD, and the serotypes of the virus were determined. Considering ELISA reslut, 62% of the samples were assigned to serotype O, 33% to serotype A, and 5% to serotype Asia-1. Furthermore, 90% of the positive samples were inoculated onto monolayer cultures of pig kidneys (IB-RS2) for isolation and antigen detection by serotype-specific ELISA kit. The great majority of detected serotype O viruses were from Esfahan province, while the most detected serotype A and serotype Asia-1 viruses were from Qom and Tehran provinces, respectively. These findings indicate that the ELISA and isolation methods are suitable for identifying and typing FMD viruses. The vaccination program in Iran, which includes three serotypes (O, A, and Asia-1), appears to be effective in controlling the spread of the disease. However, the continued circulation of these serotypes in most provinces suggests that ongoing surveillance and vaccination efforts are necessary.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"87-96"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudorabies virus as a zoonosis: scientific and public health implications. 作为人畜共患病的伪狂犬病毒：对科学和公共卫生的影响。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-12-18 DOI: 10.1007/s11262-024-02122-2

Yumei Chen, Jie Gao, Rongqian Hua, Gaiping Zhang

引用次数: 0

Detection and molecular epidemiology of canine parvovirus and identification of highly pathogenic CPV-2c isolates from Shandong, China. 山东犬细小病毒检测、分子流行病学及高致病性CPV-2c分离株鉴定

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-12-03 DOI: 10.1007/s11262-024-02125-z

Jiahui Li, Baoyu Cheng, Zihe Li, Yanlei Cui, Haiyan Yang, Weiquan Liu, Chuanmei Zhang, Yongle Yu

{"title":"Detection and molecular epidemiology of canine parvovirus and identification of highly pathogenic CPV-2c isolates from Shandong, China.","authors":"Jiahui Li, Baoyu Cheng, Zihe Li, Yanlei Cui, Haiyan Yang, Weiquan Liu, Chuanmei Zhang, Yongle Yu","doi":"10.1007/s11262-024-02125-z","DOIUrl":"10.1007/s11262-024-02125-z","url":null,"abstract":"Canine parvovirus (CPV) is an important pathogen of dogs and wild carnivores. It is a single-stranded DNA virus with a high mutation frequency and antigenic drift. To research the prevalence and genetic variation of CPV in Shandong, 62 samples from diseased dogs were collected and examined by using PCR for parvovirus. Our results showed that the positivity was 62.9% (n = 39), VP2 gene were sequenced and compared with reference strains. For the parvovirus subtype prevalence, 7 strains were CPV-2a (17.9%) and 32 strains were CPV-2c (82.1%). The results of phylogenetic analysis of VP2 gene of the CPVs showed all 39 isolates formed a major clade and were distantly related to the commercial vaccine strains. By comparing amino acid (aa) sequences, this study discovered new mutations not previously reported which may be related to host range and antigenicity. Moreover, one CPV-2c strain (QN-55) was isolated and cultured on F81 cells, and characterized by whole-genome sequencing. The TCID50 of this strain was 10-3.2/0.1 mL and animal tests have shown that the strain is fatal to infected dogs.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"97-109"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142774601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0