Virus Genes最新文献_第5页

Small RNA sequencing analysis reveals regulation of microRNA expression in Madin-Darby canine kidney epithelial cells infected with Canid alphaherpesvirus 1. 小核糖核酸测序分析揭示了感染犬α疱疹病毒 1 的马丹达比犬肾上皮细胞中微小核糖核酸表达的调控。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-17 DOI: 10.1007/s11262-024-02091-6

Maha Ben Hamouda, Angela Pearson

{"title":"Small RNA sequencing analysis reveals regulation of microRNA expression in Madin-Darby canine kidney epithelial cells infected with Canid alphaherpesvirus 1.","authors":"Maha Ben Hamouda, Angela Pearson","doi":"10.1007/s11262-024-02091-6","DOIUrl":"10.1007/s11262-024-02091-6","url":null,"abstract":"Canid alphaherpesvirus 1 (CHV-1) infection can cause spontaneous abortions in pregnant dams, and in young puppies, fatal systemic infections are common. MicroRNAs (miRNAs) affect viral infection by binding to messenger RNAs, and inhibiting expression of host and/or viral genes. We conducted deep sequencing of small RNAs in CHV-1-infected and mock-infected Madin-Darby Canine Kidney (MDCK) epithelial cells, and detected sequences corresponding to 282 cellular miRNAs. Of these, 18 were significantly upregulated at 12 h post-infection, most of which were encoded on the X chromosome. We next quantified the mature forms of several of the miRNAs using stem loop RT-qPCR. Our results revealed a discordance between the levels of small RNAs corresponding to canine miRNAs, and levels of the corresponding mature miRNAs, which suggests a block in miRNA biogenesis in infected cells. Nevertheless, we identified several mature miRNAs that exhibited a statistically significant increase upon infection. These included cfa-miR-8908b, a miRNA of unknown function, and cfa-miR-146a, homologs of which target innate immune pathways and are known to play a role in other viral infections. Interestingly, ontology analysis predicted that cfa-miR-8908b targets factors involved in the ubiquitin-like protein conjugation pathway and peroxisome biogenesis among other cellular functions. This is the first study to evaluate changes in miRNA levels upon CHV-1 infection. Based on our findings, we developed a model whereby CHV-1 infection results in changes in levels of a limited number of cellular miRNAs that target elements of the host immune response, which may provide clues regarding novel therapeutic targets.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"537-548"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor. 丙型肝炎病毒的治疗方法：基于反义寡核苷酸的候选囊壳抑制剂的硅学设计。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-31 DOI: 10.1007/s11262-024-02088-1

Burcu Hasturk, Fatih Eren

{"title":"A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor.","authors":"Burcu Hasturk, Fatih Eren","doi":"10.1007/s11262-024-02088-1","DOIUrl":"10.1007/s11262-024-02088-1","url":null,"abstract":"Direct-acting antiviral (DAA) drugs have been shown to effectively reduce viral load and cure a high proportion of hepatitis C virus (HCV) infections. However, costs associated with the course of therapy and any possible adverse effects should also be considered. It is important to acknowledge, moreover, that certain groups may not be eligible for treatment. Given that there is currently no approved vaccine for HCV infection, the need for an effective, safe, and accessible treatment remains a crucial priority. The aim of this study is to develop an antisense oligonucleotide (ASO)-based therapeutic drug that can inhibit HCV capsid. After analyzing 817 HCV capsid protein mRNA sequences using the NCBI Virus Data Portal, a conserved region of 7 nucleotides (nt) was identified in all genotypes (1-7). However, because of its high GC% content, this region is not a suitable target for ASO. Conversely, the other highly conserved region, which is only 8 nt long, was preserved in 801 datasets after removing missing and differing sequence data. The candidate ASO was then investigated using computer simulations to assess its potential. Thus, it is possible that the ASO sequence consisting of 8 nt could be a viable therapeutic target for the inhibition of HCV capsid. Furthermore, the 7 nt sequence, which is conserved in all datasets, may be targeted using alternative strategies in lieu of ASO-based targeting.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"446-454"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aquaporin-3 is down-regulated by LMP1 in nasopharyngeal carcinoma cells to regulate cell migration and affect EBV latent infection. 鼻咽癌细胞中的 Aquaporin-3 受 LMP1 下调，从而调节细胞迁移并影响 EBV 潜伏感染。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI: 10.1007/s11262-024-02096-1

Jing Li, Duo Shi, Zhiyuan Gong, Wen Liu, Yan Zhang, Bing Luo

引用次数: 0

Identification of a novel monopartite begomovirus associated with leaf curl disease of Citharexylum spinosum in India. 鉴定与印度 Citharexylum spinosum 卷叶病相关的一种新型单分型begomovirus。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-06-27 DOI: 10.1007/s11262-024-02087-2

Damini Diksha, V Kavi Sidharthan, V K Baranwal

引用次数: 0

Lineage and sublineage analysis of human papillomavirus types 51 and 59 in Iranian women. 伊朗妇女中人类乳头瘤病毒 51 型和 59 型的系谱和亚系分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1007/s11262-024-02089-0

Kimia Sharifian, Zabihollah Shoja, Somayeh Jalilvand

引用次数: 0

Isolation and identification of a pigeonpox virus strain and study on the integration of reticuloendotheliosis virus sequence. 鸽痘病毒株的分离和鉴定以及网状内皮病病毒序列整合研究。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-06-12 DOI: 10.1007/s11262-024-02084-5

Huadong Liu, Tingting Li, Juan Tang, Shurong Ding, Dongcai Wang, Juan Zhang, Yibo Yan, Kaifang Li, Xueli Guo

{"title":"Isolation and identification of a pigeonpox virus strain and study on the integration of reticuloendotheliosis virus sequence.","authors":"Huadong Liu, Tingting Li, Juan Tang, Shurong Ding, Dongcai Wang, Juan Zhang, Yibo Yan, Kaifang Li, Xueli Guo","doi":"10.1007/s11262-024-02084-5","DOIUrl":"10.1007/s11262-024-02084-5","url":null,"abstract":"In order to study the integration of reticuloendotheliosis virus (REV) in pigeonpox virus (PPV), we collected suspected pigeonpox disease material, amplified the 4b core protein gene of PPV, the gp90 gene of REV, and the integrated sequence fragments from the end of the ORF201 segment of PPV to the beginning of the LTR of REV, and sequenced these genes. The results showed that a 4b core protein fragment of 332 bp was amplified and identified as pigeonpox virus, which was named SX/TY/LTR 01/2023. Sequence analysis showed that the pigeonpox virus isolate belonged to genotype A2, which was the closest to the domestic CVL strain, with a identity of 99.4%. A band of 1191 bp was amplified from the gp90 gene of REV, named SX/TY/PPV-REV01/2023, and sequence analysis indicated that REV belonged to genotype III. The sequence analysis showed that REV belonged to genotype III, and belonged to the same large branch as the domestic isolates JSRD0701 and LNR0801, with 99.3% identity. The integrated sequence fragment was amplified to a band of 637 bp, which determined that the REV sequence was integrated in the PPV rather than a mixed infection of the two viruses. This indicates that REV was integrated in this isolation of PPV, suggesting that pigeon farms need to prevent reticuloendotheliosis at the same time when preventing pigeonpox.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"510-516"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141312263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the proteins encoded by a recently emerged cotton-infecting Polerovirus. 最近出现的感染棉花的 Polerovirus 所编码蛋白质的特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-06-21 DOI: 10.1007/s11262-024-02086-3

Mary F Akinyuwa, Bailee K Price, Sung-Hwan Kang

{"title":"Characterization of the proteins encoded by a recently emerged cotton-infecting Polerovirus.","authors":"Mary F Akinyuwa, Bailee K Price, Sung-Hwan Kang","doi":"10.1007/s11262-024-02086-3","DOIUrl":"10.1007/s11262-024-02086-3","url":null,"abstract":"The cotton leafroll dwarf virus (CLDV), an important viral pathogen responsible for substantial losses in cotton crops, has recently emerged in the United States (US). Although CLDV shares similarities with other members of the genus Polerovirus in terms of encoded proteins, their functional characteristics remain largely unexplored. In this study, we expressed and analyzed each protein encoded by CLDV to determine its intracellular localization using fluorescence protein fusion. We also evaluated their potential to induce plant responses, such as the induction of hypersensitive response-like necrosis and the generation of reactive oxygen species. Our findings show that the proteins encoded by CLDV exhibit comparable localization patterns and elicit similar robust plant responses as observed with cognate proteins from other viruses within the genus Polerovirus. This study contributes to our understanding of the functional repertoire of genes carried by Polerovirus members, particularly to CLDV that has recently emerged as a widespread viral pathogen infecting cotton in the US.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"563-567"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular functions of HAX1 during disease progress. 疾病进展过程中 HAX1 的分子功能

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-11 DOI: 10.1007/s11262-024-02081-8

Dajun Zhang, Jinke Yang, Qi Huang, Dengshuai Zhao, Tianyu Wang, Dixi Yu, Limei Qin, Keshan Zhang

引用次数: 0

Immunization of recombinant NS3 protein (protease region) of dengue virus induces high levels of CTLA-4 and apoptosis in splenocytes of BALB/c mice. 登革病毒重组 NS3 蛋白（蛋白酶区）免疫可诱导高水平 CTLA-4 和 BALB/c 小鼠脾细胞凋亡。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-08-05 DOI: 10.1007/s11262-024-02095-2

Víctor Adolfo Romero-Cruz, Angel Ramos-Ligonio, Karen García-Alejandro, Melissa Cerecedo-García, María de la Soledad Lagunes-Castro, Aracely López-Monteon

{"title":"Immunization of recombinant NS3 protein (protease region) of dengue virus induces high levels of CTLA-4 and apoptosis in splenocytes of BALB/c mice.","authors":"Víctor Adolfo Romero-Cruz, Angel Ramos-Ligonio, Karen García-Alejandro, Melissa Cerecedo-García, María de la Soledad Lagunes-Castro, Aracely López-Monteon","doi":"10.1007/s11262-024-02095-2","DOIUrl":"10.1007/s11262-024-02095-2","url":null,"abstract":"DENV infection outcomes depend on the host's variable expression of immune receptors and mediators, leading to either resolution or exacerbation. While the NS3 protein is known to induce robust immune responses, the specific impact of its protease region epitopes remains unclear. This study investigated the effect of recombinant NS3 protease region proteins from all four DENV serotypes on splenocyte activation in BALB/c mice (n = 5/group). Mice were immunized with each protein, and their splenocytes were subsequently stimulated with homologous antigens. We measured the expression of costimulatory molecules (CD28, CD80, CD86, CD152) by flow cytometry, along with IL-2 production, CD25 expression, and examined the antigen-specific activation of CD4 + and CD8 + T cells. Additionally, the expression of IL-1, IL-10, and TGF-β1 in splenocytes from immunized animals was assessed. Apoptosis was evaluated using Annexin V/PI staining and DNA fragmentation analysis. Stimulation of splenocytes from immunized mice triggered apoptosis (phosphatidylserine exposure and caspase 3/7 activation) and increased costimulatory molecule expression, particularly CD152. Low IL-2 production and low CD25 expression, as well as sustained expression of the IL-10 gene. These results suggest that these molecules might be involved in mechanisms by which the NS3 protein contributes to viral persistence and disease pathogenesis.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"475-487"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of two novel complete genomes of myxoma virus vaccine strains. 肌瘤病毒疫苗株两种新型完整基因组的比较分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-11 DOI: 10.1007/s11262-024-02090-7

Sofya Volodina, Ilya Titov, Sergey Zhivoderov, Sergey Yurkov, Alexander Malogolovkin

引用次数: 0