Virus Genes最新文献_第6页

Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea. 两种新型镰刀菌病毒共同感染单一植物病原真菌 Botrytis cinerea 分离物的特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-08 DOI: 10.1007/s11262-024-02073-8

Aqeel Ahmed, Haris Ahmed Khan, Atif Jamal, Nasar Virk, Muhammad Faraz Bhatti

{"title":"Characterization of two novel fusariviruses co-infecting a single isolate of phytopathogenic fungus Botrytis cinerea.","authors":"Aqeel Ahmed, Haris Ahmed Khan, Atif Jamal, Nasar Virk, Muhammad Faraz Bhatti","doi":"10.1007/s11262-024-02073-8","DOIUrl":"10.1007/s11262-024-02073-8","url":null,"abstract":"A wide diversity of mycoviruses has been reported from Botrytis species, some with the potential to suppress the pathogenic abilities of this fungus. Considering their importance, this study was devised to find potential hypovirulence-associated mycoviruses found in Botrytis cinerea strains isolated from Pakistani strawberry fields. Here we report the complete genome characterization of two fusariviruses co-infecting a single isolate of phytopathogenic fungus B. cinerea (Kst14a). The viral genomes were sequenced by deep sequencing using total RNA fractions of the Kst14a isolate. The identified viruses were tentatively named Botrytis cinerea fusarivirus 9 (BcFV9) and Botrytis cinerea fusarivirus 3a (BcFV3a). Both viruses had a single-segmented (ssRNA) genome having a size of 6424 and 8370 nucleotides encoding two discontinuous open reading frames (ORFs). ORF-1 of both mycoviruses encodes for a polyprotein having a conserved domain of RNA-dependent RNA polymerase (RdRP) and a helicase domain (Hel) which function in RNA replication, while ORF2 encodes a hypothetical protein with an unknown function, respectively. Phylogenetic analysis indicated that BcFV9 made a clade with the genus Alphafusarivirus and BcFV3a fall in the genus Betafusarivirus in the family Fusariviridae. To our knowledge, this is the first report of two fusariviruses identified in isolates of B. cinerea from Pakistan. Both mycoviruses successfully transfected to a compatible strain of B. cinerea (Mst11). A comparison of virus-free (VF) and virus-infected (VI) isogenic lines showed the presence of these viruses was causing hypovirulence in infected strains. Virus-infected strains also had a small lesion size while testing the pathogenicity via apple assay.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"402-411"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140877864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of human astrovirus infection in children under 5 years of age with acute gastroenteritis in Tehran, Iran, 2021-2022: co-infection with rotavirus. 2021-2022 年伊朗德黑兰 5 岁以下急性肠胃炎儿童感染人类星状病毒的分子特征：与轮状病毒合并感染。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-15 DOI: 10.1007/s11262-024-02075-6

Atefeh Kachooei, Mahtab Mirhoseinian, Somayeh Jalilvand, Tayebeh Latifi, Mahsa Feizi, Zahra Shahosseini, Arash Arashkia, Sayed Mahdi Marashi, Zabihollah Shoja

{"title":"Molecular characterization of human astrovirus infection in children under 5 years of age with acute gastroenteritis in Tehran, Iran, 2021-2022: co-infection with rotavirus.","authors":"Atefeh Kachooei, Mahtab Mirhoseinian, Somayeh Jalilvand, Tayebeh Latifi, Mahsa Feizi, Zahra Shahosseini, Arash Arashkia, Sayed Mahdi Marashi, Zabihollah Shoja","doi":"10.1007/s11262-024-02075-6","DOIUrl":"10.1007/s11262-024-02075-6","url":null,"abstract":"Human astroviruses (HAstVs) are considered important causative pathogens of acute gastroenteritis (AGE) in children under 5 years of age worldwide, along with group A rotavirus (RVA), norovirus (NoV), and enteric adenovirus (EAdV). The present study was aimed to both detect HAstV and its co-infections and investigate genetic analysis of circulating HAstV and co-infected virus in hospitalized children under 5 years of age with AGE in Iran. Accordingly, a sum of 200 stool specimens were screened by PCR for HAstV during 2021-2022. The HAstV was found in 0.5% of 200 specimens (n = 1) while was co-infected with RVA. The genetic and phylogenetic analysis indicated HAstV1 genotype, which clustered with viruses from lineage 1b, which has not been previously reported in Iran. The detected RVA strain belonged to G1 lineage II/P[8]-lineage III, which has been reported previously in Iran as the most common strain. The further genetic analysis of RVA VP6 and NSP4 demonstrated an atypical genotype pattern G1P[8]-I1-E2, as a mono-reassortant of a Wa-like genogroup, which appeared to be reassorted with the NSP4 gene of E2 genotype of the G2P[4] DS-1 genogroup. Although the clinical outcomes of the AGE-causing viruses co-infection is not yet entirely clear, it seems that future studies will be helpful to merge clinical and epidemiological data of co-infecting viruses for a more accurate medical and clinical relevance in symptomatic children.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"357-369"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential emerging role of piRNA/PIWI complex in virus infection. piRNA/PIWI 复合物在病毒感染中的潜在作用。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-06-04 DOI: 10.1007/s11262-024-02078-3

Yanyan Li, Kai Wang, Wen Liu, Yan Zhang

引用次数: 0

Transient expression analysis of promoters of okra enation leaf curl virus in Nicotiana benthamiana, cotton and okra plants. 秋葵卷叶病毒启动子在烟草、棉花和秋葵植物中的瞬时表达分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-10 DOI: 10.1007/s11262-024-02074-7

Zainul A Khan, Kanika Gupta, Indranil Dasgupta

引用次数: 0

Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma. 潜伏膜蛋白 2A 对鼻咽癌 GCNT3 表达的调控机制研究

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s11262-024-02071-w

Yijing Chen, Yan Zhang, Shi Duo, Wen Liu, Bing Luo

引用次数: 0

Phylogenetic analysis and molecular genetic characteristics of West Nile virus lineage 2 isolates circulating in the Russian Federation. 俄罗斯联邦流行的西尼罗河病毒 2 系分离株的系统发育分析和分子遗传特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-06-07 DOI: 10.1007/s11262-024-02079-2

A S Antonov, I M Shpak, D V Ustinov, M P Izhberdeeva, A N Guseva, A Y Galkina, N V Borodai, S K Udovichenko, A V Toporkov

{"title":"Phylogenetic analysis and molecular genetic characteristics of West Nile virus lineage 2 isolates circulating in the Russian Federation.","authors":"A S Antonov, I M Shpak, D V Ustinov, M P Izhberdeeva, A N Guseva, A Y Galkina, N V Borodai, S K Udovichenko, A V Toporkov","doi":"10.1007/s11262-024-02079-2","DOIUrl":"10.1007/s11262-024-02079-2","url":null,"abstract":"Since its initial detection in Africa, the West Nile virus has disseminated widely across all continents, becoming endemic in numerous countries, including the Russian Federation. A substantial expansion of the West Nile virus range was observed in the European part of the Russian territory in 1999. In light of this epidemiological trend, research endeavours focusing on monitoring West Nile virus circulation activity in endemic regions of the country have gained paramount significance. A substantial dataset has been accrued from 2007 onwards regarding genomic variability and dissemination dynamics across the country throughout the entire monitoring period for the West Nile fever pathogen. The objective of this study was to characterise West Nile virus isolates that have been circulating in the Russian Federation and identify their molecular and genetic characteristics. A phylogenetic analysis of 55 complete genome sequences revealed that the West Nile virus population within the Russian Federation is genetically heterogeneous and is represented by four major clades. One of these clades is currently exhibiting extensive spread into new regions of the country.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"370-376"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spillover of Newcastle disease virus to Himalayan Griffon vulture: a possible food-based transmission. 新城疫病毒向喜马拉雅狮鹫的扩散：一种可能的食物传播。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s11262-024-02072-9

Shinjini Bhattacharya, Pankaj Deka, Sangeeta Das, Samshul Ali, Bhaskar Choudhury, Parikshit Kakati, Sachin Kumar

引用次数: 0

H6N2 reassortant avian influenza virus isolate in wild birds in Jiangxi Province, China. 中国江西省野鸟中的 H6N2 重型禽流感病毒分离株。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-05-09 DOI: 10.1007/s11262-024-02068-5

Yingjie Wang, Xiang Li, Xinru Lv, Yi Li, Qing An, Yang Xiu, Xiangtong Lv, Haozhuo Song, Changyu Zhou, Qiushi Xu, Dan Qiao, Siyuan Yang, Hongliang Chai, Xiangwei Zeng

{"title":"H6N2 reassortant avian influenza virus isolate in wild birds in Jiangxi Province, China.","authors":"Yingjie Wang, Xiang Li, Xinru Lv, Yi Li, Qing An, Yang Xiu, Xiangtong Lv, Haozhuo Song, Changyu Zhou, Qiushi Xu, Dan Qiao, Siyuan Yang, Hongliang Chai, Xiangwei Zeng","doi":"10.1007/s11262-024-02068-5","DOIUrl":"10.1007/s11262-024-02068-5","url":null,"abstract":"H6 avian influenza virus is widely prevalent in wild birds and poultry and has caused human infection in 2013 in Taiwan, China. During our active influenza surveillance program in wild waterfowl at Poyang Lake, Jiangxi Province, an H6N2 AIV was isolated and named A/bean goose/JiangXi/452-4/2013(H6N2). The isolate was characterized as a typical low pathogenic avian influenza virus (LPAIV) due to the presence of the amino acid sequence PQIETR↓GLFGAI at the cleavage site of the hemagglutinin (HA) protein. The genetic evolution analysis revealed that the NA gene of the isolate originated from North America and exhibited the highest nucleotide identity (99.29%) with a virus recovered from wild bird samples in North America, specifically A/bufflehead/California/4935/2012(H11N2). Additionally, while the HA and PB1 genes belonged to the Eurasian lineage, they displayed frequent genetic interactions with the North American lineage. The remaining genes showed close genetic relationships with Eurasian viruses. The H6N2 isolate possessed a complex genome, indicating it is a multi-gene recombinant virus with genetic material from both Eurasian and North American lineages.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"320-324"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amsacta moorei entomopoxvirus encodes a protein kinase with dual activity and a broad substrate spectrum including two putative cellular substrates. Amsacta moorei entomopoxvirus编码的蛋白激酶具有双重活性和广泛的底物谱，包括两种假定的细胞底物。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-05-04 DOI: 10.1007/s11262-024-02069-4

Hacer Muratoğlu, Remziye Nalcacioglu, Basil M Arif, Zihni Demirbag

{"title":"Amsacta moorei entomopoxvirus encodes a protein kinase with dual activity and a broad substrate spectrum including two putative cellular substrates.","authors":"Hacer Muratoğlu, Remziye Nalcacioglu, Basil M Arif, Zihni Demirbag","doi":"10.1007/s11262-024-02069-4","DOIUrl":"10.1007/s11262-024-02069-4","url":null,"abstract":"Amsacta moorei entomopoxvirus (AMEV) is a poxvirus that can only infect insects. This virus is an attractive research material because it is similar to smallpox virus. AMEV is one of many viruses that encode protein kinases that drive the host's cellular mechanisms, modifying immune responses to it, and regulating viral protein activity. We report here the functional characterization of a serine/threonine (Ser/Thr) protein kinase (PK) gene (ORF AMV197) of AMEV. Expression of the AMV197 gene in baculovirus expression system yielded a ~ 35.5 kDa protein. PK activity of expressed AMV197 was shown by standard PK assay. Substrate profiling of AMV197 protein by peptide microarray indicated that the expressed protein phosphorylated 81 of 624 substrates which belong to 28 families of PK substrates. While the hypothetical AMV197 protein phosphorylates Ser/Thr only, we demonstrated that the expressed PK also phosphorylates probes with tyrosine residues on the array which is a rare property among PKs. Pull-down assay of the AMV197 protein with the subcellular protein fractionations of Ld652 cells showed that it is using two cellular proteins (18 and 42 kDa) as novel putative substrates. Our results suggest that AMEV can regulate cellular mechanisms by phosphorylating cellular proteins through AMV197 PK. However, further experiments are needed to identify the exact role of this PK in the replication of AMEV.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"287-294"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and whole-genome sequence analysis of Balagodu virus in Japan. 日本 Balagodu 病毒的分离和全基因组序列分析。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-03-16 DOI: 10.1007/s11262-024-02060-z

Mayumi Honda, Hironori Setoyama, Ryosuke Nabekura, Katsunori Murota, Yuto Suda, Tohru Yanase

引用次数: 0