Virus Genes最新文献_第7页

Study on the regulatory mechanism of latent membrane protein 2A on GCNT3 expression in nasopharyngeal carcinoma. 潜伏膜蛋白 2A 对鼻咽癌 GCNT3 表达的调控机制研究

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s11262-024-02071-w

Yijing Chen, Yan Zhang, Shi Duo, Wen Liu, Bing Luo

引用次数: 0

Phylogenetic analysis and molecular genetic characteristics of West Nile virus lineage 2 isolates circulating in the Russian Federation. 俄罗斯联邦流行的西尼罗河病毒 2 系分离株的系统发育分析和分子遗传特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-06-07 DOI: 10.1007/s11262-024-02079-2

A S Antonov, I M Shpak, D V Ustinov, M P Izhberdeeva, A N Guseva, A Y Galkina, N V Borodai, S K Udovichenko, A V Toporkov

{"title":"Phylogenetic analysis and molecular genetic characteristics of West Nile virus lineage 2 isolates circulating in the Russian Federation.","authors":"A S Antonov, I M Shpak, D V Ustinov, M P Izhberdeeva, A N Guseva, A Y Galkina, N V Borodai, S K Udovichenko, A V Toporkov","doi":"10.1007/s11262-024-02079-2","DOIUrl":"10.1007/s11262-024-02079-2","url":null,"abstract":"Since its initial detection in Africa, the West Nile virus has disseminated widely across all continents, becoming endemic in numerous countries, including the Russian Federation. A substantial expansion of the West Nile virus range was observed in the European part of the Russian territory in 1999. In light of this epidemiological trend, research endeavours focusing on monitoring West Nile virus circulation activity in endemic regions of the country have gained paramount significance. A substantial dataset has been accrued from 2007 onwards regarding genomic variability and dissemination dynamics across the country throughout the entire monitoring period for the West Nile fever pathogen. The objective of this study was to characterise West Nile virus isolates that have been circulating in the Russian Federation and identify their molecular and genetic characteristics. A phylogenetic analysis of 55 complete genome sequences revealed that the West Nile virus population within the Russian Federation is genetically heterogeneous and is represented by four major clades. One of these clades is currently exhibiting extensive spread into new regions of the country.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"370-376"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spillover of Newcastle disease virus to Himalayan Griffon vulture: a possible food-based transmission. 新城疫病毒向喜马拉雅狮鹫的扩散：一种可能的食物传播。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-08-01 Epub Date: 2024-05-13 DOI: 10.1007/s11262-024-02072-9

Shinjini Bhattacharya, Pankaj Deka, Sangeeta Das, Samshul Ali, Bhaskar Choudhury, Parikshit Kakati, Sachin Kumar

引用次数: 0

H6N2 reassortant avian influenza virus isolate in wild birds in Jiangxi Province, China. 中国江西省野鸟中的 H6N2 重型禽流感病毒分离株。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-05-09 DOI: 10.1007/s11262-024-02068-5

Yingjie Wang, Xiang Li, Xinru Lv, Yi Li, Qing An, Yang Xiu, Xiangtong Lv, Haozhuo Song, Changyu Zhou, Qiushi Xu, Dan Qiao, Siyuan Yang, Hongliang Chai, Xiangwei Zeng

{"title":"H6N2 reassortant avian influenza virus isolate in wild birds in Jiangxi Province, China.","authors":"Yingjie Wang, Xiang Li, Xinru Lv, Yi Li, Qing An, Yang Xiu, Xiangtong Lv, Haozhuo Song, Changyu Zhou, Qiushi Xu, Dan Qiao, Siyuan Yang, Hongliang Chai, Xiangwei Zeng","doi":"10.1007/s11262-024-02068-5","DOIUrl":"10.1007/s11262-024-02068-5","url":null,"abstract":"H6 avian influenza virus is widely prevalent in wild birds and poultry and has caused human infection in 2013 in Taiwan, China. During our active influenza surveillance program in wild waterfowl at Poyang Lake, Jiangxi Province, an H6N2 AIV was isolated and named A/bean goose/JiangXi/452-4/2013(H6N2). The isolate was characterized as a typical low pathogenic avian influenza virus (LPAIV) due to the presence of the amino acid sequence PQIETR↓GLFGAI at the cleavage site of the hemagglutinin (HA) protein. The genetic evolution analysis revealed that the NA gene of the isolate originated from North America and exhibited the highest nucleotide identity (99.29%) with a virus recovered from wild bird samples in North America, specifically A/bufflehead/California/4935/2012(H11N2). Additionally, while the HA and PB1 genes belonged to the Eurasian lineage, they displayed frequent genetic interactions with the North American lineage. The remaining genes showed close genetic relationships with Eurasian viruses. The H6N2 isolate possessed a complex genome, indicating it is a multi-gene recombinant virus with genetic material from both Eurasian and North American lineages.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"320-324"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amsacta moorei entomopoxvirus encodes a protein kinase with dual activity and a broad substrate spectrum including two putative cellular substrates. Amsacta moorei entomopoxvirus编码的蛋白激酶具有双重活性和广泛的底物谱，包括两种假定的细胞底物。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-05-04 DOI: 10.1007/s11262-024-02069-4

Hacer Muratoğlu, Remziye Nalcacioglu, Basil M Arif, Zihni Demirbag

{"title":"Amsacta moorei entomopoxvirus encodes a protein kinase with dual activity and a broad substrate spectrum including two putative cellular substrates.","authors":"Hacer Muratoğlu, Remziye Nalcacioglu, Basil M Arif, Zihni Demirbag","doi":"10.1007/s11262-024-02069-4","DOIUrl":"10.1007/s11262-024-02069-4","url":null,"abstract":"Amsacta moorei entomopoxvirus (AMEV) is a poxvirus that can only infect insects. This virus is an attractive research material because it is similar to smallpox virus. AMEV is one of many viruses that encode protein kinases that drive the host's cellular mechanisms, modifying immune responses to it, and regulating viral protein activity. We report here the functional characterization of a serine/threonine (Ser/Thr) protein kinase (PK) gene (ORF AMV197) of AMEV. Expression of the AMV197 gene in baculovirus expression system yielded a ~ 35.5 kDa protein. PK activity of expressed AMV197 was shown by standard PK assay. Substrate profiling of AMV197 protein by peptide microarray indicated that the expressed protein phosphorylated 81 of 624 substrates which belong to 28 families of PK substrates. While the hypothetical AMV197 protein phosphorylates Ser/Thr only, we demonstrated that the expressed PK also phosphorylates probes with tyrosine residues on the array which is a rare property among PKs. Pull-down assay of the AMV197 protein with the subcellular protein fractionations of Ld652 cells showed that it is using two cellular proteins (18 and 42 kDa) as novel putative substrates. Our results suggest that AMEV can regulate cellular mechanisms by phosphorylating cellular proteins through AMV197 PK. However, further experiments are needed to identify the exact role of this PK in the replication of AMEV.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"287-294"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and whole-genome sequence analysis of Balagodu virus in Japan. 日本 Balagodu 病毒的分离和全基因组序列分析。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-03-16 DOI: 10.1007/s11262-024-02060-z

Mayumi Honda, Hironori Setoyama, Ryosuke Nabekura, Katsunori Murota, Yuto Suda, Tohru Yanase

引用次数: 0

Donkey-like kirkovirus is associated with diarrhea in piglets. 驴样柯科病毒与仔猪腹泻有关。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.1007/s11262-024-02066-7

Panpan Tong, Zunbao Wang, Yueyi Dang, Lei Zhang, Guangwei Song, Xiaozhen Song, Juanjuan Pan, Ling Kuang, Junhui Li, Gang Lu, Jinxin Xie

{"title":"Donkey-like kirkovirus is associated with diarrhea in piglets.","authors":"Panpan Tong, Zunbao Wang, Yueyi Dang, Lei Zhang, Guangwei Song, Xiaozhen Song, Juanjuan Pan, Ling Kuang, Junhui Li, Gang Lu, Jinxin Xie","doi":"10.1007/s11262-024-02066-7","DOIUrl":"10.1007/s11262-024-02066-7","url":null,"abstract":"Kirkovirus (kirV), a seemingly novel virus family, has been found in horses and donkeys. The study's objectives are to investigate the presence of the virus in swine. In this study, donkey-like kirV was detected in rectal swabs of piglets with diarrhea, and the positive rate was found to be 100% (149/149). However, this virus was detected in only one of 261 clinically healthy piglets, which suggested a strong relationship between the kirV and the diarrheic disease. We obtained the whole-genome sequences of three kirVs (Cj-D5, Cj-D32, and Cj-D43), with a length of 3750 nucleotides (nt) and sharing 99.9% nt identity with donkey kirVs. Furthermore, the three viruses shared 88.5-100% and 23-51% of the Rep protein sequence, similar to available reference strains of Kirkoviridae and Circoviridae, respectively. Moreover, like horse and donkey kirVs, the RCR domain and P-loop NTPase domains of Rep protein and nonanucleotide motif (CAATATTAC) of the three viruses were similar to those of Circoviruses and Cycloviruses. Phylogenetic analysis showed that these viruses could be grouped with members in the proposed family Kirkoviridae. This is the first report to describe that kirV can circulate in piglets with diarrhea, and future studies are needed to determine the pathogenesis of this virus.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"314-319"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovine adenoviruses infecting sheep and goats in Türkiye: detection and molecular characterization of three different types. 感染土尔其绵羊和山羊的绵羊腺病毒：三种不同类型的检测和分子特征。

IF 1.6 4区医学

Virus Genes Pub Date : 2024-06-01 Epub Date: 2024-03-15 DOI: 10.1007/s11262-024-02063-w

Ilke Karayel-Hacioglu, Buket Gul, Deniz Acun Yildiz, Feray Alkan

{"title":"Ovine adenoviruses infecting sheep and goats in Türkiye: detection and molecular characterization of three different types.","authors":"Ilke Karayel-Hacioglu, Buket Gul, Deniz Acun Yildiz, Feray Alkan","doi":"10.1007/s11262-024-02063-w","DOIUrl":"10.1007/s11262-024-02063-w","url":null,"abstract":"Adenoviruses (AdVs) have been detected in a wide variety of animals. To date, eight types of AdVs in sheep and two types in goats have been identified, which belong to two distinct genera, Mastadenovirus and Atadenovirus. Typically, the term pneumo-enteritis is used to describe adenovirus-induced disease in small ruminants, which has been associated with both enteric and respiratory symptoms of varying severity. The aim of this study was to detect and identify AdVs of small ruminants belonging to the genera Mastadenovirus and Atadenovirus. For this purpose, diagnostic samples (47 lung, 27 intestine, and two pooled tissue samples including intestine and lung) from 49 small ruminants (39 sheep and 10 goats) were used. Following the viral DNA extraction, PCR was carried out by using the primers targeting the hexon gene in order to detect both mast- and atadenoviruses. Sequencing the amplified fragments revealed the presence of three types of ovine adenovirus (OAdV): OAdV-3, OAdV-4, and OAdV-8. Specifically, OAdV-3 was detected in two sheep and a goat while OAdV-4 and OAdV-8 were found in only one sheep each. There is still limited data on the interaction between the viruses in different adenovirus genera and the detected disease, as well as the genetic diversity of adenoviruses, especially in small ruminants. In conclusion, the detection of AdVs in lung and intestinal tissues of small ruminants in this study suggests that these viruses may have contributed to the disease and/or predisposed to other agents.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"309-313"},"PeriodicalIF":1.6,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140141095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leucoma salicis nucleopolyhedrovirus (LesaNPV) genome sequence shed new light on the origin of the Alphabaculovirus orpseudotsugatae species 水蛭核多聚核酸病毒（LesaNPV）基因组序列揭示了阿尔法巴库尔病毒（Alphabaculovirus orpseudotsugatae）物种起源的新线索

IF 1.6 4区医学

Virus Genes Pub Date : 2024-04-09 DOI: 10.1007/s11262-024-02062-x

Martyna Krejmer-Rabalska, Lukasz Rabalski, Maciej Kosinski, Iwona Skrzecz, Jadwiga Ziemnicka, Boguslaw Szewczyk

{"title":"Leucoma salicis nucleopolyhedrovirus (LesaNPV) genome sequence shed new light on the origin of the Alphabaculovirus orpseudotsugatae species","authors":"Martyna Krejmer-Rabalska, Lukasz Rabalski, Maciej Kosinski, Iwona Skrzecz, Jadwiga Ziemnicka, Boguslaw Szewczyk","doi":"10.1007/s11262-024-02062-x","DOIUrl":"https://doi.org/10.1007/s11262-024-02062-x","url":null,"abstract":"LesaNPV (Leucoma salicis nucleopolyhedrovirus) is an alphabaculovirus group Ib. Potentially, it can be an eco-friendly agent to control the white satin moth Leucoma salicis population. In this study, we have established the relationship between LesaNPV and other closely related alphabaculoviruses. Environmental samples of late instar of white satin moth collected in Poland infected with baculovirus have been homogenized, polyhedra were purified and subjected to scanning and transmission electron microscopy. Viral DNA was sequenced using the Illumina platform and the whole-genome sequence was established by de novo assembly of paired reads. Genome annotation and phylogenetic analyses were performed with the use of bioinformatics tools. The genome of LesaNPV is 132 549 bp long with 154 ORFs and 54.9% GC content. Whole-genome sequencing revealed deletion of dUTPase as well as ribonucleoside reductases small and large subunits region in LesaNPV genome compared to Dasychira pudibunda nucleopolyhedrovirus (DapuNPV) and Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV) where this region is complete. Phylogenetic analysis of Baculoviridae family members showed that LesaNPV is less divergent from a common ancestor than closely related species DapuNPV and OpMNPV. This is interesting because their hosts do not occur in the same area. The baculoviruses described in this manuscript are probably isolates of one species and could be assigned to recently denominated species Alphabaculovirus orpseudotsugatae, historically originating from OpMNPV. This finding could have significant implications for the classification and understanding of the phylogeographical spread of baculoviruses.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140561874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave 从岩溶洞穴中分离出的新型噬菌体 D6（Pseudomonas syringae pv. tomato bacteriophage D6）的特征和全基因组分析

IF 1.6 4区医学

Virus Genes Pub Date : 2024-04-09 DOI: 10.1007/s11262-024-02064-9

Qingshan Wu, Ni An, Zheng Fang, Shixia Li, Lan Xiang, Qiuping Liu, Leitao Tan, Qingbei Weng

{"title":"Characteristics and whole-genome analysis of a novel Pseudomonas syringae pv. tomato bacteriophage D6 isolated from a karst cave","authors":"Qingshan Wu, Ni An, Zheng Fang, Shixia Li, Lan Xiang, Qiuping Liu, Leitao Tan, Qingbei Weng","doi":"10.1007/s11262-024-02064-9","DOIUrl":"https://doi.org/10.1007/s11262-024-02064-9","url":null,"abstract":"Pseudomonas syringae is a gram-negative plant pathogen that infects plants such as tomato and poses a threat to global crop production. In this study, a novel lytic phage infecting P. syringae pv. tomato DC3000, named phage D6, was isolated and characterized from sediments in a karst cave. The latent period of phage D6 was found to be 60 min, with a burst size of 16 plaque-forming units per cell. Phage D6 was stable at temperatures between 4 and 40 °C but lost infectivity when heated to 70 °C. Its infectivity was unaffected at pH 6–10 but became inactivated at pH ≤ 5 or ≥ 12. The genome of phage D6 is a linear double-stranded DNA of 307,402 bp with a G + C content of 48.43%. There is a codon preference between phage D6 and its host, and the translation of phage D6 gene may not be entirely dependent on the tRNA library provided by the host. A total of 410 open reading frames (ORFs) and 14 tRNAs were predicted in its genome, with 92 ORFs encoding proteins with predicted functions. Phage D6 showed low genomic similarity to known phage genomes in the GenBank and Viral sequence databases. Genomic and phylogenetic analyses revealed that phage D6 is a novel phage. The tomato plants were first injected with phage D6, and subsequently with Pst DC3000, using the foliar spraying and root drenching inoculum approach. Results obtained after 14 days indicated that phage D6 inoculation decreased P. syringae-induced symptoms in tomato leaves and inhibited the pathogen’s growth in the leaves. The amount of Pst DC3000 was reduced by 150- and 263-fold, respectively. In conclusion, the lytic phage D6 identified in this study belongs to a novel phage within the Caudoviricetes class and has potential for use in biological control of plant diseases.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":"41 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140561786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0