Virus Genes最新文献_第8页

Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines. 利用 YBX3 基因敲除和过表达细胞系，探索 YBX3 在 PEDV 感染中的作用。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s11262-024-02109-z

Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang

{"title":"Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.","authors":"Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang","doi":"10.1007/s11262-024-02109-z","DOIUrl":"10.1007/s11262-024-02109-z","url":null,"abstract":"Porcine epidemic diarrhea (PED) is a highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV), which results in significant economic losses. PEDV infection causes severe damage to the midgut barrier in the small intestine. YBX3, an important protein in tight junctions, promotes epithelial cell proliferation. However, its role in the process of PEDV infection remains unclear. In this study, we observed a significant increase in mRNA expression of YBX3 following PEDV infection. Additionally, the protein expression of YBX3 showed an initial increase followed by a decrease over time. Furthermore, treatment with 2% DSS resulted in a significant down-regulation of YBX3 mRNA and protein expression. Furthermore, we successfully generated knockout and overexpression cell lines of YBX3. Preliminary assays indicated that elevated expression of YBX3 inhibited the PEDV replication, while knockout of YBX3 had the opposite effect. In conclusion, our study has preliminarily revealed the functional role of YBX3 during PEDV infection. This finding lays the foundation for further investigation into its mechanism in future and also provides new insights into the mechanism of PEDV-host interactions.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"667-673"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of the novel phage SAP71 and its potential use against Staphylococcus aureus in an atopic dermatitis mouse model. 新型噬菌体 SAP71 的分离及其在特应性皮炎小鼠模型中对抗金黄色葡萄球菌的潜力。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-05 DOI: 10.1007/s11262-024-02106-2

Huaixin Geng, Xin Yang, Chenghui Zou, Wen Zhang, Jingheng Xiang, Kailang Yang, Yi Shu, Guangxin Luan, Xu Jia, Mao Lu

引用次数: 0

Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen. 表达orf病毒的疫苗病毒H3L跨膜蛋白类似物F1L，并将其成功纯化为诊断抗原。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-13 DOI: 10.1007/s11262-024-02097-0

Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal

{"title":"Expression of F1L, a vaccinia virus H3L transmembrane protein analogue of orf virus, and its successful purification as a diagnostic antigen.","authors":"Poulinlu Golmei, Gnanavel Venkatesan, Anand Kushwaha, Amit Kumar, B Mondal","doi":"10.1007/s11262-024-02097-0","DOIUrl":"10.1007/s11262-024-02097-0","url":null,"abstract":"Orf or contagious ecthyma is a highly contagious, zoonotic, and economically important global viral disease of small ruminants and is endemic in India. Vaccination of susceptible goats/sheep along with suitable recombinant protein-based serological assay will be useful in the control of the infection. In this study, the full-length and truncated versions of F1L encoding gene (ORF 059) of orf virus were cloned into pFasBac HT A vector, transformed in DH10Bac cells, and expressed in insect cells. The full-length and truncated recombinant F1L proteins were expressed as a 6 × histidine-tagged fusion protein for ease of purification by Ni-NTA affinity chromatography under denaturing conditions. A protein with ~ 40 kDa and ~ 35 kDa for full-length and truncated F1L protein, respectively, were expressed and confirmed by SDS-PAGE and western blot. The protein reactivity evaluated by western blot analysis and indirect ELISA using ORFV hyperimmune serum was also found to be reactive. The results of the present study showed that the purified recombinant F1L protein can be used as a diagnostic antigen in sero-surveillance of ORFV infection in small ruminants. To the best of authors' knowledge, this is the first report on the expression of ORFV F1L in insect cells using a baculovirus vector and its successful purification to use as the potential diagnostic antigen in ELISA.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"642-651"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141972236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm. 在一个奶牛场的环境样本中检测到 H5N1 高致病性禽流感病毒并确定其特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-15 DOI: 10.1007/s11262-024-02085-4

Gagandeep Singh, Jessie D Trujillo, Chester D McDowell, Franco Matias-Ferreyra, Sujan Kafle, Taeyong Kwon, Natasha N Gaudreault, Isaac Fitz, Lance Noll, Igor Morozov, Jamie Retallick, Juergen A Richt

{"title":"Detection and characterization of H5N1 HPAIV in environmental samples from a dairy farm.","authors":"Gagandeep Singh, Jessie D Trujillo, Chester D McDowell, Franco Matias-Ferreyra, Sujan Kafle, Taeyong Kwon, Natasha N Gaudreault, Isaac Fitz, Lance Noll, Igor Morozov, Jamie Retallick, Juergen A Richt","doi":"10.1007/s11262-024-02085-4","DOIUrl":"10.1007/s11262-024-02085-4","url":null,"abstract":"The recent expansion of HPAIV H5N1 infections in terrestrial mammals in the Americas, most recently including the outbreak in dairy cattle, emphasizes the critical need for better epidemiological monitoring of zoonotic diseases. In this work, we detected, isolated, and characterized the HPAIV H5N1 from environmental swab samples collected from a dairy farm in the state of Kansas, USA. Genomic sequencing of these samples uncovered two distinctive substitutions in the PB2 (E249G) and NS1 (R21Q) genes which are rare and absent in recent 2024 isolates of H5N1 circulating in the mammalian and avian species. Additionally, approximately 1.7% of the sequence reads indicated a PB2 (E627K) substitution, commonly associated with virus adaptation to mammalian hosts. Phylogenetic analyses of the PB2 and NS genes demonstrated more genetic identity between this environmental isolate and the 2024 human isolate (A/Texas/37/2024) of H5N1. Conversely, HA and NA gene analyses revealed a closer relationship between our isolate and those found in other dairy cattle with almost 100% identity, sharing a common phylogenetic subtree. These findings underscore the rapid evolutionary progression of HPAIV H5N1 among dairy cattle and reinforces the need for more epidemiological monitoring which can be done using environmental sampling.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"517-527"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11686969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular epidemiology and genetic evolution of avian influenza H5N1 subtype in Nigeria, 2006 to 2021. 2006 年至 2021 年尼日利亚 H5N1 亚型禽流感的分子流行病学和基因演变。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-06-19 DOI: 10.1007/s11262-024-02080-9

Ridwan O Adesola, Bernard A Onoja, Andrew M Adamu, Sheriff T Agbaje, Modinat D Abdulazeez, Olalekan C Akinsulie, Adetolase Bakre, Oyelola A Adegboye

{"title":"Molecular epidemiology and genetic evolution of avian influenza H5N1 subtype in Nigeria, 2006 to 2021.","authors":"Ridwan O Adesola, Bernard A Onoja, Andrew M Adamu, Sheriff T Agbaje, Modinat D Abdulazeez, Olalekan C Akinsulie, Adetolase Bakre, Oyelola A Adegboye","doi":"10.1007/s11262-024-02080-9","DOIUrl":"10.1007/s11262-024-02080-9","url":null,"abstract":"Nigeria recorded one of the earliest outbreaks of the Highly Pathogenic Avian Influenza (HPAI) virus H5N1 in 2006, which spread to other African countries. In 2023, 18 countries reported outbreaks of H5N1 in poultry, with human cases documented in Egypt, Nigeria, and Djibouti. There is limited information on the molecular epidemiology of HPAI H5N1 in Nigeria. We determined the molecular epidemiology and genetic evolution of the virus from 2006 to 2021. We investigated the trend and geographical distribution across Nigeria. The evolutionary history of 61 full-length genomes was performed from 13 countries worldwide, and compared with sequences obtained from the early outbreaks in Nigeria up to 2021. MEGA 11 was used to determine the phylogenetic relationships of H5N1 strains, which revealed close ancestry between sequences in Nigeria and those from other African countries. Clade classification was performed using the subspecies classification tool for Bacterial and Viral Bioinformatics Research Center (BV-BRC) version 3.35.5. H5N1 Clade 2.2 was observed in 2006, with 2.3.2, 2.3.2.1f clades observed afterwards and 2.3.4.4b in 2021. Our findings underscore the need for genomics surveillance to track antigenic variation and clades switching to monitor the epidemiological of the virus and safeguard human and animal health.Impacts Specific variations in the hemagglutinin (HA) and neuraminidase (NA) genes of Avian influenza virus are consistent in different geographical regions. H5N1 Clade 2.2 was reported in 2006, with 2.3.2, 2.3.2.1f afterwards and 2.3.4.4b in 2021. Nigeria is an epicentre for avian influenza with three major migratory routes for wild birds transversing the country. It is plausible that the Avian influenza in Northern Nigeria may be linked to wild bird sanctuaries in the region.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"501-509"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11383836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141421780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequences of two tombusvirus-like viruses identified in Echinacea purpurea seeds. 在紫锥菊种子中发现的两种类墓病毒的完整基因组序列。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-18 DOI: 10.1007/s11262-024-02092-5

Juhyun Kim, Eun Jin Jeon, Minji Jun, Da-Som Lee, Seong-Jin Lee, Seungmo Lim

引用次数: 0

The complete genome of equid herpesvirus-1 (EHV-1) field isolates from Argentina reveals an interspecific recombinant strain. 阿根廷马疱疹病毒-1（EHV-1）野外分离株的完整基因组揭示了一个种间重组株。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-19 DOI: 10.1007/s11262-024-02093-4

Rocio Lucia Tau, Ana Eugenia Marandino, Yanina Panzera, Florencia Alamos, Maria Aldana Vissani, Sonia Alejandra Romera, Ruben Pérez, Silvina Soledad Maidana

引用次数: 0

Characterization of an envelope protein 118L in invertebrate iridescent virus 6 (IIV6). 无脊椎动物虹彩病毒 6（IIV6）包膜蛋白 118L 的特征。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-06-26 DOI: 10.1007/s11262-024-02082-7

Betul Altun, Kubra Zengin, Sevde Yayli Dabag, Aydin Yesilyurt, Remziye Nalcacioglu, Zihni Demirbag

{"title":"Characterization of an envelope protein 118L in invertebrate iridescent virus 6 (IIV6).","authors":"Betul Altun, Kubra Zengin, Sevde Yayli Dabag, Aydin Yesilyurt, Remziye Nalcacioglu, Zihni Demirbag","doi":"10.1007/s11262-024-02082-7","DOIUrl":"10.1007/s11262-024-02082-7","url":null,"abstract":"Invertebrate iridescent virus 6 (IIV6) is a nucleocytoplasmic insect virus and a member of the family Iridoviridae. The IIV6 genome consists of 212,482 bp of linear dsDNA with 215 non-overlapping and putative protein-encoding ORFs. The IIV6 118L ORF is conserved in all sequenced members of the Iridoviridae and encodes a 515 amino acid protein with three predicted transmembrane domains and several N-glycosylation/N-myristoylation sites. In this study, we characterized the 118L ORF by both deleting it from the viral genome and silencing its expression with dsRNA in infected insect cells. The homologous recombination method was used to replace 118L ORF with the green fluorescent protein (gfp) gene. Virus mutants in which the 118L gene sequence had been replaced with gfp were identified by fluorescence microscopy but could not be propagated separately from the wild-type virus in insect cells. Unsuccessful attempts to isolate the mutant virus with the 118L gene deletion suggested that the protein is essential for virus replication. To support this result, we used dsRNA to target the 118L gene and showed that treatment resulted in a 99% reduction in virus titer. Subsequently, we demonstrated that 118L-specific antibodies produced against the 118L protein expressed in the baculovirus vector system were able to neutralize the virus infection. All these results indicate that 118L is a viral envelope protein that is required for the initiation of virus replication.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"549-558"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small RNA sequencing analysis reveals regulation of microRNA expression in Madin-Darby canine kidney epithelial cells infected with Canid alphaherpesvirus 1. 小核糖核酸测序分析揭示了感染犬α疱疹病毒 1 的马丹达比犬肾上皮细胞中微小核糖核酸表达的调控。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-17 DOI: 10.1007/s11262-024-02091-6

Maha Ben Hamouda, Angela Pearson

{"title":"Small RNA sequencing analysis reveals regulation of microRNA expression in Madin-Darby canine kidney epithelial cells infected with Canid alphaherpesvirus 1.","authors":"Maha Ben Hamouda, Angela Pearson","doi":"10.1007/s11262-024-02091-6","DOIUrl":"10.1007/s11262-024-02091-6","url":null,"abstract":"Canid alphaherpesvirus 1 (CHV-1) infection can cause spontaneous abortions in pregnant dams, and in young puppies, fatal systemic infections are common. MicroRNAs (miRNAs) affect viral infection by binding to messenger RNAs, and inhibiting expression of host and/or viral genes. We conducted deep sequencing of small RNAs in CHV-1-infected and mock-infected Madin-Darby Canine Kidney (MDCK) epithelial cells, and detected sequences corresponding to 282 cellular miRNAs. Of these, 18 were significantly upregulated at 12 h post-infection, most of which were encoded on the X chromosome. We next quantified the mature forms of several of the miRNAs using stem loop RT-qPCR. Our results revealed a discordance between the levels of small RNAs corresponding to canine miRNAs, and levels of the corresponding mature miRNAs, which suggests a block in miRNA biogenesis in infected cells. Nevertheless, we identified several mature miRNAs that exhibited a statistically significant increase upon infection. These included cfa-miR-8908b, a miRNA of unknown function, and cfa-miR-146a, homologs of which target innate immune pathways and are known to play a role in other viral infections. Interestingly, ontology analysis predicted that cfa-miR-8908b targets factors involved in the ubiquitin-like protein conjugation pathway and peroxisome biogenesis among other cellular functions. This is the first study to evaluate changes in miRNA levels upon CHV-1 infection. Based on our findings, we developed a model whereby CHV-1 infection results in changes in levels of a limited number of cellular miRNAs that target elements of the host immune response, which may provide clues regarding novel therapeutic targets.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"537-548"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141629285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor. 丙型肝炎病毒的治疗方法：基于反义寡核苷酸的候选囊壳抑制剂的硅学设计。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-10-01 Epub Date: 2024-07-31 DOI: 10.1007/s11262-024-02088-1

Burcu Hasturk, Fatih Eren

{"title":"A therapeutic approach for the hepatitis C virus: in silico design of an antisense oligonucleotide-based candidate capsid inhibitor.","authors":"Burcu Hasturk, Fatih Eren","doi":"10.1007/s11262-024-02088-1","DOIUrl":"10.1007/s11262-024-02088-1","url":null,"abstract":"Direct-acting antiviral (DAA) drugs have been shown to effectively reduce viral load and cure a high proportion of hepatitis C virus (HCV) infections. However, costs associated with the course of therapy and any possible adverse effects should also be considered. It is important to acknowledge, moreover, that certain groups may not be eligible for treatment. Given that there is currently no approved vaccine for HCV infection, the need for an effective, safe, and accessible treatment remains a crucial priority. The aim of this study is to develop an antisense oligonucleotide (ASO)-based therapeutic drug that can inhibit HCV capsid. After analyzing 817 HCV capsid protein mRNA sequences using the NCBI Virus Data Portal, a conserved region of 7 nucleotides (nt) was identified in all genotypes (1-7). However, because of its high GC% content, this region is not a suitable target for ASO. Conversely, the other highly conserved region, which is only 8 nt long, was preserved in 801 datasets after removing missing and differing sequence data. The candidate ASO was then investigated using computer simulations to assess its potential. Thus, it is possible that the ASO sequence consisting of 8 nt could be a viable therapeutic target for the inhibition of HCV capsid. Furthermore, the 7 nt sequence, which is conserved in all datasets, may be targeted using alternative strategies in lieu of ASO-based targeting.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"446-454"},"PeriodicalIF":1.9,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0