Virus Genes最新文献_第8页

Lumpy skin disease: a systematic review of mode of transmission, risk of emergence, and risk entry pathways. 结节性皮肤病：传播方式、出现风险和风险进入途径的系统回顾。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-22 DOI: 10.1007/s11262-024-02117-z

Bhawanpreet Kaur, Sehajpal Singh Dhillon, Amarpreet Singh Pannu, C S Mukhopadhyay

引用次数: 0

Domestic cat hepadnavirus genotype B is present in Southern Brazil. 巴西南部存在家猫狂犬病毒基因 B 型。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-14 DOI: 10.1007/s11262-024-02115-1

Alaíse Tessmann, Juliana Sumienski, Alexandre Sita, Larissa Mallmann, Gabriela Espíndola Birlem, Nilson Júnior da Silva Nunes, Camila Gottlieb Lupion, Juliana Schaeffer Eckert, Meriane Demoliner, Juliana Schons Gularte, Paula Rodrigues de Almeida, Fernando Rosado Spilki, Matheus Nunes Weber

{"title":"Domestic cat hepadnavirus genotype B is present in Southern Brazil.","authors":"Alaíse Tessmann, Juliana Sumienski, Alexandre Sita, Larissa Mallmann, Gabriela Espíndola Birlem, Nilson Júnior da Silva Nunes, Camila Gottlieb Lupion, Juliana Schaeffer Eckert, Meriane Demoliner, Juliana Schons Gularte, Paula Rodrigues de Almeida, Fernando Rosado Spilki, Matheus Nunes Weber","doi":"10.1007/s11262-024-02115-1","DOIUrl":"10.1007/s11262-024-02115-1","url":null,"abstract":"Domestic cat hepadnavirus (DCH) (Orthohepadnavirus felisdomestici) is an emerging virus related to the hepatitis B virus (HBV) already reported in many countries. The molecular prevalence of DCH varies widely in the regions investigated so far. In the present work, we reported the presence of DCH in Brazil. Sixty cat serum samples tested by DCH presence using PCR and 1.67% (1/60) were positive, similar to the low positive molecular rates reported in United States and Japan. The DCH full-length genome was classified in genotype B, which is uncommon since this genotype was only reported once in Japan. The DCH-positive sample was obtained in a stray cat female apparently healthy, presenting ALT, AST, and ALKP normal values, and negative for FIV and FeLV. Due the low positivity rate detected, some factors as alteration in hepatic enzymes and FIV/FeLV infection could not be evaluated. Other works are necessary to statistically validate these observations in Brazil.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"81-86"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and genome analysis of Klebsiella phages with lytic activity against Klebsiella pneumoniae. 对肺炎克雷伯氏菌具有溶菌活性的克雷伯氏菌噬菌体的特征和基因组分析。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-11-15 DOI: 10.1007/s11262-024-02123-1

Shanzheng Bi, Hong Peng, Xiao Wei, Changjun Wang, Xiangna Zhao

引用次数: 0

Genetic characterization of a novel HIV-1 circulating recombinant form (CRF162_cpx) involving CRF01_AE, CRF07_BC and subtype B in Guangdong, China. 中国广东一种涉及CRF01_AE、CRF07_BC和B亚型的新型HIV-1循环重组形式（CRF162_cpx）的遗传特征

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-12-16 DOI: 10.1007/s11262-024-02127-x

Yun Lan, Linghua Li, Mingfeng Xiao, Yaqing Lin, Xuemei Ling, Feng Li, Fengyu Hu

{"title":"Genetic characterization of a novel HIV-1 circulating recombinant form (CRF162_cpx) involving CRF01_AE, CRF07_BC and subtype B in Guangdong, China.","authors":"Yun Lan, Linghua Li, Mingfeng Xiao, Yaqing Lin, Xuemei Ling, Feng Li, Fengyu Hu","doi":"10.1007/s11262-024-02127-x","DOIUrl":"10.1007/s11262-024-02127-x","url":null,"abstract":"Human immunodeficiency virus type 1 (HIV-1) is characterized by its extremely high level of genetic diversity. The spread of different subtypes in the same population often leads to the emergence of circulating recombinant forms (CRFs) and unique recombinant forms (URFs). At present, the main recombinant subtypes of HIV-1 in China originate from CRF07_BC, CRF01_AE, CRF55_01B and subtype B. Here, we obtained the nearly full-length genomes (NFLGs) from eight HIV-1 infected patients in Guangdong Province, which shared highly similar recombinant patterns, involving two CRF01_AE, one CRF07_BC and two subtype B segments. The eight NFLG sequences own four similar breakpoints as follows: 1220 nucleotide (nt), 2243 nt, 2673 nt, and 5820 nt according to the HXB2 reference sequence, and they therefore were assigned as CRF162_cpx. This is the first complex CRF derived from CRF01_AE, CRF07_BC and subtype B in China. The Bayesian inference of the segments showed that HIV-1 CRF162_cpx was inferred to have approximately originated around 2010-2015. The emergence of CRF162_cpx indicates that the HIV diversity in southeast China constantly accumulates and evolves. Thus, intensive surveillance of HIV-1 molecular epidemiology should be reinforced.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"136-143"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The H5N1-NS1 protein affects the host cell cycle and apoptosis through interaction with the host lncRNA PIK3CD-AS2. H5N1-NS1 蛋白通过与宿主 lncRNA PIK3CD-AS2 相互作用，影响宿主细胞周期和细胞凋亡。

IF 1.9 4区医学

Virus Genes Pub Date : 2025-02-01 Epub Date: 2024-10-18 DOI: 10.1007/s11262-024-02118-y

Man Zhang, Yingyue Zeng, Qingqing Liu, Feng Li, Jian Zhao, Zhikui Liu, Hongsheng Liu, Huawei Feng

{"title":"The H5N1-NS1 protein affects the host cell cycle and apoptosis through interaction with the host lncRNA PIK3CD-AS2.","authors":"Man Zhang, Yingyue Zeng, Qingqing Liu, Feng Li, Jian Zhao, Zhikui Liu, Hongsheng Liu, Huawei Feng","doi":"10.1007/s11262-024-02118-y","DOIUrl":"10.1007/s11262-024-02118-y","url":null,"abstract":"Long noncoding RNAs (lncRNAs) are involved in the host antiviral response, but how host lncRNAs interact with viral proteins remains unclear. The NS1 protein of avian influenza viruses can affect the interferon-dependent expression of several host lncRNAs, but the exact mechanism is unknown. To further investigate the molecular mechanism and functions of NS1 proteins and host lncRNAs, we performed RNA-immunoprecipitation sequencing assays on A549 cells transfected with the H5N1-NS1 gene. We identified multiple sets of host lncRNAs that interact with NS1. The results of the RNA pulldown assay indicated that PIK3CD-AS2 can directly interact with NS1 in vitro. Immunofluorescence confocal microscopy showed that these proteins were colocalized in the nucleus. Further studies revealed that PIK3CD-AS2 can also inhibit the transcription of NS1, which in turn affects the translation of the NS1 protein. PIK3CD-AS2 overexpression regulates NS1 protein-induced cell cycle arrest and initiates apoptosis. We hope this work will help elucidate the molecular mechanisms associated with NS1 proteins in the study of viral infections to promote the development of potential treatments for patients infected with avian influenza A viruses.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"38-53"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatic identification of monkeypox virus phylogenetic gene trees that are representative of its whole-genome phylogenetic tree. 通过生物信息学鉴定猴痘病毒系统发育基因树，这些基因树是猴痘病毒全基因组系统发育树的代表。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-10-06 DOI: 10.1007/s11262-024-02110-6

El-Miracle Idorenyin Akpan, Diana Diaz-Cánova, Malachy Ifeanyi Okeke

{"title":"Bioinformatic identification of monkeypox virus phylogenetic gene trees that are representative of its whole-genome phylogenetic tree.","authors":"El-Miracle Idorenyin Akpan, Diana Diaz-Cánova, Malachy Ifeanyi Okeke","doi":"10.1007/s11262-024-02110-6","DOIUrl":"10.1007/s11262-024-02110-6","url":null,"abstract":"Phylogenetic analysis based on whole-genome sequences is the gold standard for monkeypox virus (MPXV) phylogeny. However, genomic epidemiology capability and capacity are lacking or limited in resource poor countries of sub-Saharan Africa. Therefore, these make real-time genome surveillance of MPXV virtually impossible. We hypothesized that phylogenetic analysis based on single, conserved genes will produce phylogenetic tree topology consistent with MPXV whole-genome phylogeny, thus serving as a reliable proxy to phylogenomic analysis. In this study, we analyzed 62 conserved MPXV genes and showed that Bayesian phylogenetic analysis based on five genes (OPG 066/E4L, OPG068/E6R, OPG079/I3L, OPG145/A18R, and OPG150/A23R) generated phylogenetic trees with 72.2-96.3% topology similarity index to the reference phylogenomic tree topology. Our results showed that phylogenetic analysis of the identified five genes singly or in combination can serve as surrogate for whole-genome phylogenetic analysis, and thus obviates the need for whole-genome sequencing and phylogenomic analysis in regions where genomic epidemiology competence and capacity are lacking or unavailable. This study is relevant to evolution and genome surveillance of MPXV in resource limited countries.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"635-641"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tackling hepatitis B Virus with CRISPR/Cas9: advances, challenges, and delivery strategies. 用 CRISPR/Cas9 处理乙型肝炎病毒：进展、挑战和传递策略。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-28 DOI: 10.1007/s11262-024-02105-3

Dakshina M Nair, Leela Kakithakara Vajravelu, Jayaprakash Thulukanam, Vishnupriya Paneerselvam, Poornima Baskar Vimala, Rahul Harikumar Lathakumari

{"title":"Tackling hepatitis B Virus with CRISPR/Cas9: advances, challenges, and delivery strategies.","authors":"Dakshina M Nair, Leela Kakithakara Vajravelu, Jayaprakash Thulukanam, Vishnupriya Paneerselvam, Poornima Baskar Vimala, Rahul Harikumar Lathakumari","doi":"10.1007/s11262-024-02105-3","DOIUrl":"10.1007/s11262-024-02105-3","url":null,"abstract":"Hepatitis B virus (HBV) infection remains a significant global health challenge, with chronic HBV leading to severe liver diseases, including cirrhosis and hepatocellular carcinoma. Current treatments often fail to eradicate the virus, highlighting the need for innovative therapeutic strategies. The CRISPR/Cas9 system has emerged as a dynamic tool for precise genome editing and presents a promising approach to targeting and eliminating HBV infection. This review provides a comprehensive overview of the advances, challenges, and delivery strategies associated with CRISPR/Cas9-based therapies for HBV. We begin by elucidating the mechanism of the CRISPR/Cas9 system and then explore HBV pathogenesis, focusing on the role of covalently closed circular DNA (cccDNA) and integrated HBV DNA in maintaining chronic infection. CRISPR/Cas9 can disrupt these key viral reservoirs, which are critical for persistent HBV replication and associated liver damage. The application of CRISPR/Cas9 in HBV treatment faces significant challenges, such as off-target effects, delivery efficiency, and immune responses. These challenges are addressed by examining current approaches to enhance the specificity, safety, and efficacy of CRISPR/Cas9. A future perspective on the development and clinical translation of CRISPR/Cas9 therapies for HBV is provided, emphasizing the requirement for further research to improve delivery methods and ensure durable safety and effectiveness. This review underscores the transformative potential of CRISPR/Cas9 in combating HBV and sets the stage for future breakthroughs in the field.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"592-602"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the study of LNPs for mRNA delivery and clinical applications. 用于 mRNA 运送和临床应用的 LNPs 研究进展。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-08-22 DOI: 10.1007/s11262-024-02102-6

Bili Wang, Biao Shen, Wenqing Xiang, Hongqiang Shen

{"title":"Advances in the study of LNPs for mRNA delivery and clinical applications.","authors":"Bili Wang, Biao Shen, Wenqing Xiang, Hongqiang Shen","doi":"10.1007/s11262-024-02102-6","DOIUrl":"10.1007/s11262-024-02102-6","url":null,"abstract":"Messenger ribonucleic acid (mRNA) was discovered in 1961 as an intermediary for transferring genetic information from DNA to ribosomes for protein synthesis. The COVID-19 pandemic brought worldwide attention to mRNA vaccines. The emergency use authorization of two COVID-19 mRNA vaccines, BNT162b2 and mRNA-1273, were major achievements in the history of vaccine development. Lipid nanoparticles (LNPs), one of the most superior non-viral delivery vectors available, have made many exciting advances in clinical translation as part of the COVID-19 vaccine and therefore has the potential to accelerate the clinical translation of many gene drugs. In addition, due to these small size, biocompatibility and excellent biodegradability, LNPs can efficiently deliver nucleic acids into cells, which is particularly important for current mRNA therapeutic regimens. LNPs are composed cationic or pH-dependent ionizable lipid bilayer, polyethylene glycol (PEG), phospholipids, and cholesterol, represents an advanced system for the delivery of mRNA vaccines. Furthermore, optimization of these four components constituting the LNPs have demonstrated enhanced vaccine efficacy and diminished adverse effects. The incorporation of biodegradable lipids enhance the biocompatibility of LNPs, thereby improving its potential as an efficacious therapeutic approach for a wide range of challenging and intricate diseases, encompassing infectious diseases, liver disorders, cancer, cardiovascular diseases, cerebrovascular conditions, among others. Consequently, this review aims to furnish the scientific community with the most up-to-date information regarding mRNA vaccines and LNP delivery systems.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"577-591"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of HDAC9-induced deacetylation of glycolysis-related GAPDH lysine 219 on rotavirus replication in rotavirus-infected Caco-2 cells. HDAC9 诱导的糖酵解相关 GAPDH 赖氨酸 219 去乙酰化对轮状病毒感染的 Caco-2 细胞中轮状病毒复制的影响。

IF 16.4 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-20 DOI: 10.1007/s11262-024-02104-4

Lijun Song, Peicheng Zhong, Runyu Yu, Yue Yuan, Yujing Zhou, Yupei Qian, Siyan Yang, Haosen Yi, Zhiyan Yang, Wenchang Zhao

{"title":"Effect of HDAC9-induced deacetylation of glycolysis-related GAPDH lysine 219 on rotavirus replication in rotavirus-infected Caco-2 cells.","authors":"Lijun Song, Peicheng Zhong, Runyu Yu, Yue Yuan, Yujing Zhou, Yupei Qian, Siyan Yang, Haosen Yi, Zhiyan Yang, Wenchang Zhao","doi":"10.1007/s11262-024-02104-4","DOIUrl":"10.1007/s11262-024-02104-4","url":null,"abstract":"Post-translational modifications (PTMs), as epigenetic modifications, are significant in the interaction between virus and its host. However, it is unclear whether rotavirus (RV) causes changes in both the host cell epigenetic protein modification and the regulatory mechanism of viral replication. Here, we analyzed the proteome of Caco-2 cells to determine if acetylation modification occurred within the cells after RV infection. We found that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a protein involved in glycolysis, was deacetylated at lysine 219 via histone deacetylase 9 (HDAC9) in 50 h after the RV infection. Remarkably, the deacetylation of GAPDH promoted RV replication. Finally, we found that glycolysis was alterable in Caco-2 cells by RV or the deacetylation of GAPDH lysine 219, using the Seahorse XF Glycolysis Stress Test. In conclusion, our results demonstrate for the first time that RV infection promoted deacetylation of GAPDH at lysine 219 in order to increase its own viral replication in Caco-2 cells.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"621-634"},"PeriodicalIF":16.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway. 猪流行性腹泻病毒 E 蛋白通过激活 PERK 和 ATF6 而非 IRE1 信号通路诱导未折叠蛋白反应。

IF 1.9 4区医学

Virus Genes Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1007/s11262-024-02108-0

Liang Zheng, Ying Yang, Mingxin Ma, Qin Hu, Zhijun Wu, Matthew Kay, Xiaoge Yang, Liwei Yin, Fusheng Ding, Hua Zhang

{"title":"Porcine epidemic diarrhea virus E protein induces unfolded protein response through activating both PERK and ATF6 rather than IRE1 signaling pathway.","authors":"Liang Zheng, Ying Yang, Mingxin Ma, Qin Hu, Zhijun Wu, Matthew Kay, Xiaoge Yang, Liwei Yin, Fusheng Ding, Hua Zhang","doi":"10.1007/s11262-024-02108-0","DOIUrl":"10.1007/s11262-024-02108-0","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) small envelope protein (E) plays important roles in virus budding, assembly, and release. Our previous study found that PEDV E protein localizes in the endoplasmic reticulum (ER) to trigger the unfolded protein response (UPR). However, how UPR is directly regulated by PEDV E protein remains elusive. Thus, in this study, we investigated the expression of ER chaperone glucose-regulated protein 78 (GRP78) and activations of the three main UPR signaling pathways to elucidate the underlying mechanisms of UPR triggered by PEDV E protein. The results showed that over-expression of PEDV E protein increased expression of GRP78 and induced stronger phosphorylation of both protein kinase RNA-like ER kinase (PERK) and eukaryotic initiation factor-2α (eIF2α), as well as caused the significant degradation of activating transcription factor 6 (ATF6), in both dose- and time-dependent manners. However, PEDV E protein did not induce UPR through the inositol-requiring enzyme 1 (IRE1) signaling pathway, as revealed by the splicing of XBP1 remaining unaffected and unchanged when PEDV E protein was overexpressed. Taken together, these results demonstrate that PEDV E protein induces UPR through activation of both PERK and ATF6 pathways rather than IRE1 signaling. This study not only provides mechanistic details of UPR induced by the PEDV E protein, but also provides insights into these new biologic functions to help us better understand the interactions between PEDV and host cells.","PeriodicalId":51212,"journal":{"name":"Virus Genes","volume":" ","pages":"652-666"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0