对肺炎克雷伯氏菌具有溶菌活性的克雷伯氏菌噬菌体的特征和基因组分析。

IF 1.9 4区医学 Q3 GENETICS & HEREDITY

Virus Genes Pub Date : 2024-11-15 DOI:10.1007/s11262-024-02123-1

Shanzheng Bi, Hong Peng, Xiao Wei, Changjun Wang, Xiangna Zhao

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引用次数: 0

摘要

肺炎克雷伯菌是一种重要的革兰氏阴性机会性病原体，可引起多种感染性疾病。由于肺炎克雷伯菌对抗生素的耐药性越来越强，使用噬菌体可能是治疗这些感染的一种非抗生素方法。本研究从医院污水中分离出了五种特异于肺炎克氏菌的噬菌体，即 2044307w、k2044hw、k2044ew、k2044302 和 2146hw，并对其进行了鉴定。根据透射电子显微镜（TEM）和基因组分析，它们属于 KP32 病毒的 A 组。这些噬菌体的宿主谱极窄。通过裂解试验、pH 值和温度稳定性测试确定了噬菌体的表型特征。这有助于加深我们对肺炎克氏菌噬菌体的了解。

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Characterization and genome analysis of Klebsiella phages with lytic activity against Klebsiella pneumoniae.

Klebsiella pneumoniae is an important gram-negative opportunistic pathogen that causes a variety of infectious diseases. As K. pneumoniae are becoming increasingly resistant to antibiotics, the use of bacteriophages may offer a non-antibiotic-based approach to treat these infections. In the present study, five lytic bacteriophages, 2044307w, k2044hw, k2044ew, k2044302 and 2146hw specific to K. pneumoniae were isolated from hospital sewage and characterized. They belong to group A of the KP32viruses based on transmission electron microscopy (TEM) and genome analysis. These bacteriophages have an extremely narrow host spectrum. The phenotypic characteristics of the phages were determined using lysis assay, pH, and temperature stability tests. This contributes to expanding our understanding of K. pneumoniae phages.

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来源期刊

Virus Genes 医学-病毒学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.