Al-Kauniyah: Jurnal Biologi最新文献

{"title":"INDEX AL-KAUNIYAH: JURNAL BIOLOGI VOL. 17 NO. 2 OKTOBER 2024","authors":"Index Oktober","doi":"10.15408/kauniyah.v17i2.38671","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i2.38671","url":null,"abstract":"","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141015678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keragaman Serbuk Sari Pada Tumbuhan Legum di Kampus IPB, Dramaga, Bogor","authors":"Tiarana Brilyanita, Nunik Sri Ariyanti, Nina Ratna Djuita","doi":"10.15408/kauniyah.v17i2.29846","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i2.29846","url":null,"abstract":"AbstrakMorfologi serbuk sari dapat dipakai untuk identifikasi tumbuhan, sumber bukti taksonomi, dan diterapkan dalam banyak disiplin ilmu. Penelitian ini bertujuan untuk menggambarkan ciri morfologi serbuk sari yang berasal dari tumbuhan legum. Sampel serbuk sari diperoleh dari bunga yang dikoleksi di area kampus. Koleksi bunga dilakukan dengan metode jelajah di kawasan kampus IPB, Dramaga. Sampel serbuk sari yang akan diamati dibuat dengan metode asetolisis. Pengukuran morfologi polen dihitung berdasarkan sumbu polar dan ekuatorial dalam skala mikrometer. Ciri morfologi serbuk sari yang diteliti meliputi bentuk, unit serbuk sari, tipe aperture, ukuran, indeks P/E, dan ornamentasi eksin. Serbuk sari diamati dengan mikroskop majemuk Olympus (CX-33) yang dilengkapi kamera indomikro. Hasil penelitian menunjukkan bahwa bentuk serbuk sari pada legum ada dua macam yaitu circular dan circular oval. Ukuran serbuk sari beragam dari sangat kecil sampai sangat besar. Serbuk sari memiliki indeks P/E > 1,00. Tipe aperture meliputi monoporate, triporate, tetraporate, tricolpate, dan tricolporate. Ornamentasi eksin yang dijumpai berupa scabrate, reticulate, dan psilate. Serbuk sari yang diamati pada penelitian ini menggambarkan morfologi yang khas sehingga dapat dijadikan karakter tambahan dalam mengidentifikasi tumbuhan legum.AbstractPollen morphology can be usefull for plant identification, sources of taxonomic evidence, and apply in many disciplines. This study aimed to describe the morphological characteristics of pollen from legume plants. Pollen samples were obtained from flowers collected in the campus area. The collection of flowers was carried out using the roaming method in the area of the IPB campus, Dramaga. The pollen sample to be observed was made by the acetolysis method. Pollen morphology measurements were calculated based on the polar and equatorial axes on the micrometer scale. The morphological characteristics of the pollen studied included pollen shape, pollen unit, aperture type, size, P/E index, and exine ornamentation. Pollen grains were observed with an Olympus compound microscope (CX-33) equipped with an indomicro camera. The results showed that there are two forms of pollen grains, namely circular and circular oval. Pollen sizes vary from very small to very large. Pollen has a P/E index > 1.00. The aperture types include monoporate, triporate, tetraporate, tricolpate, and tricolporate. The exine ornamentation found is scabrate, reticulate, and psilate. The pollen observed in this study shows a distinctive morphology so that it can be used as an additional character in identifying legume plant. ","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141015831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Bacillus thuringiensis Biolarvicide and Temephos Synthetic Larvicides on Culex quinquefasciatus Larvae","authors":"Niken Subekti, Citra Anisah Zahra, Anita Fadhila","doi":"10.15408/kauniyah.v17i2.32788","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i2.32788","url":null,"abstract":"AbstractAn open environment with drainage and lush green grass around the hotel allows mosquitoes to breed. Mosquitoes from the genus Culex are one of the vectors for transmitting arboviruses and filariasis. Temephos is an active ingredient often used to control Culex quinquefasciatus and considered as environmental pollution. Therefore, it is necessary to develop environmentally friendly larvicides, such as the Bacillus thuringiensis biolarvicide. Many studies were conducted to control Aedes aegypti mosquito larvae using these microbial agents, but very little for controlling Cx. quinquefasciatus mosquito larvae. This study aims to compare the effectiveness of temephos and B. thuringiensis biolarvicides. Cx. quinquefasciatus larvae were divided into the insect sample group with temephos and the B. thuringiensis biolarvicide group at concentrations of 0.01, 0.02, and 0.03 mg/L. The number of dead larvae was calculated at 1, 2, 3, 4, 5, 6, and 24 hours. Data analysis was performed using probit analysis of lethal time (LT50 and LT90). From statistical analysis, B. thuringiensis as larvicides showed 100% mortality of mosquito larvae. B. thuringiensis biolarvicide can be used as a substitute for chemical larvicide since it is proven effective in killing Cx. quinquefasciatus mosquito larvae in 24 hours and is environmentally friendly.AbstrakLingkungan terbuka dengan sistem pembuangan dan rumput yang hijau di sekitar hotel memungkinkan nyamuk berkembang biak. Nyamuk dari genus Culex adalah salah satu vektor yang mengirimkan arbovirus dan filariasis. Temephos adalah bahan aktif yang sering digunakan untuk mengendalikan Culex quinquefasciatus dan dianggap mencemari lingkungan. Oleh karena itu, perlu untuk mengembangkan larvasida yang ramah lingkungan, seperti Bacillus thuringiensis biolarvasida. Banyak penelitian yang dilakukan untuk mengendalikan larva nyamuk Aedes aegypti menggunakan agen mikroba ini, tetapi sangat sedikit untuk mengendalikan larva nyamuk Cx. quinquefasciatus. Penelitian ini bertujuan untuk membandingkan efektivitas temephos dan B. thuringiensis biolarvasida. Larva Cx. quinquefasciatus dibagi menjadi kelompok sampel serangga dengan temephos dan kelompok B. thuringiensis biolarvasida pada konsentrasi 0,01, 0,02, dan 0,03 mg/L. Jumlah larva yang mati dihitung pada 1, 2, 3, 4, 5, 6, dan 24 jam. Analisis data dilakukan analisis waktu letal probit (LT50 dan LT90). Analisis statistik, B. thuringiensis sebagai larvasida menunjukkan 100% kematian larva nyamuk. B. thuringiensis biolarvasida dapat digunakan sebagai pengganti larvasida kimia karena terbukti efektif dalam membunuh larva nyamuk Cx. quinquefasciatus dalam waktu 24 jam dan ramah lingkungan.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141017185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kajian Morfologi dan Hubungan Panjang Dengan Berat Udang Mantis, Harpiosquilla raphidea (Fabricius, 1798)","authors":"Nurul Oktaviani, Winda Dwi Kartika, Tia Wulandari, Fitriya Shalehati","doi":"10.15408/kauniyah.v17i1.29651","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.29651","url":null,"abstract":"AbstrakUdang mantis (Harpiosquilla raphidea) memiliki karakter morfologi yang berbeda dengan jenis udang lainnya. Selain itu H. raphidea dikenal sebagai hewan air yang mempunyai nilai ekonomis tinggi, khususnya di kawasan mangrove Tanjung Jabung Barat Provinsi Jambi. Pemanfaatan H. raphidea secara ekonomis dianggap perlu diselaraskan dengan kajian morfologi, khususnya yang terkait dengan hubungan panjang dan berat untuk mendukung nilai jual. Penelitian ini bertujuan untuk menganalisis morfologi dan pola pertumbuhan H. raphidea. Sampel dikoleksi dari perairan mangrove Pangkal Babu Desa Tungkal 1 Tanjung Jabung Barat. Analisis morfologi menunjukkan bahwa H. raphidea memiliki ciri khas yaitu adanya maksiliped (lengan predator) bercakar tajam dan tidak terdapat perbedaan morfologi antara individu jantan dan betina. H. raphidea yang paling banyak dikoleksi berukuran 168–203mm. Pola pertumbuhan H. raphidea  adalah alometrik negatif yang artinya pertambahan panjang lebih cepat dibandingkan pertambahan beratnya baik untuk individu jantan maupun betina. Sehingga dapat disimpulkan bahwa hubungan panjang dan berat H. raphidea dari Perairan Pangkal Babu Tanjung Jabung Barat menunjukkan pola pertumbuhan yang sejalan dengan H. raphidea dari kawasan perairan lainnya di Indonesia.AbstractMantis shrimp (Harpiosquilla raphidea) has morphological characteristics that are different from other types of shrimp. Apart from that, H. raphidea is known as an aquatic animal that has high economic value, especially in the West Tanjung Jabung mangrove area, Jambi Province. The economic use of H. raphidea is considered to need to be harmonized with morphological studies, especially those related to the relationship between length and weight to support selling value. This study aims to analyze the morphology and growth patterns of H. raphidea. Samples were collected from the mangrove waters of Pangkal Babu, Tungkal 1 Tanjung Jabung Barat Village. Morphological analysis shows that H. raphidea has distinctive characteristics, namely the presence of maxilipeds (predatory arms) with sharp claws and there are no morphological differences between male and female individuals. The most commonly collected H. raphidea measures 168–203mm. The growth pattern of H. raphidea is negative allometric, which means that the increase in length is faster than the increase in weight for both male and female individuals. So it can be concluded that the relationship between length and weight of H. raphidea from the Pangkal Babu waters of West Tanjung Jabung shows a growth pattern that is in line with H. raphidea from other water areas in Indonesia.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respon Pertumbuhan dan Produksi Padi (Oryza sativa L.) Pada Kombinasi Pupuk Organik Granular dan Anorganik","authors":"Ardian Khairiah, Sulyanah Sulyanah, Dasumiati Dasumiati","doi":"10.15408/kauniyah.v17i1.35754","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.35754","url":null,"abstract":"AbstrakPenggunaan pupuk anorganik dalam budi daya tanaman yang berlebihan dapat mengakibat kerusakan lingkungan. Pemberian Pupuk Organik Granul (POG) diharapkan dapat mengurangi penggunaan pupuk anorganik sekaligus meningkatkan produksi padi Galur Mukti Padi (GMP) 04. Penelitian bertujuan untuk mengetahui pengaruh kombinasi penggunaan POG dan pupuk anorganik terhadap pertumbuhan dan produksi padi GMP 04, dan memperoleh dosis POG yang dapat mengurangi penggunaan pupuk anorganik. Penelitian menggunakan Rancangan Acak Kelompok (RAK) split plot dua faktor. Faktor pertama dosis POG (0, 70, dan 140 g/m2) dan faktor kedua dosis pupuk anorganik (0, 25, 50, dan 100% dari dosis rekomendasi). Data dianalisis dengan uji ANOVA menggunakan aplikasi SAS. Hasil menunjukkan pemberian POG dosis 70 g/m2 dan 140 g/m2 berpengaruh terhadap pertumbuhan dan produksi padi GMP 04 (P <0,05). Perlakuan POG 140 g/m2 menghasilkan persentase gabah isi tertinggi. Kombinasi antara POG dan pupuk anorganik berpengaruh nyata pada parameter jumlah gabah isi/malai, bobot kering/rumpun dan bobot 500 bulir/rumpun (P <0,05). Perlakuan POG 70 g/m2+pupuk anorganik 100% menghasilkan jumlah gabah isi/malai, bobot kering/rumpun, dan bobot 500 bulir/rumpun yang lebih tinggi dari perlakuan lainnya. Penambahan POG belum mampu  mengurangi penggunaan pupuk anorganik, namun dapat memaksimalkan pertumbuhan dan produksi padi GMP 04 pada dosis 70 g/m2.AbstractExcessive use of inorganic fertilizers in plant cultivation can result in environmental damage. The provision of Granulated Organic Fertilizer (POG) is expected to reduce the use of inorganic fertilizer while increasing production of Mukti Padi (GMP) 04 rice strains. The research aims to determine the effect of the combination of using POG and inorganic fertilizer on the growth and production of GMP 04 rice, and to obtain the correct dose of POG. can reduce the use of inorganic fertilizers. The research used a split plot randomized block design (RAK) with two factors. The first factor is the dose of POG (0, 70, and 140 g/m2) and the second factor is the dose of inorganic fertilizer (0, 25, 50, and 100 of the recommended dose). Data were analyzed using the ANOVA test using the SAS application. The results showed that giving POG doses of 70 g/m2 and 140 g/m2 had an effect on the growth and production of GMP 04 rice (P<0.05). Treatment G2 (140 g/m2) produced the highest percentage of filled grain. The combination of POG and inorganic fertilizer had a significant effect on the parameters of number of filled grains/panicle, dry weight/clump and weight of 500 grains/clump (P<0.05). The G1A3 treatment (70 g/m2, 100%) produced a higher number of filled grains/panicles, dry weight/clump, and weight of 500 grains/clump than other treatments. The addition of POG has not been able to reduce the use of inorganic fertilizer, but it can maximize the growth and production of GMP 04 rice at a dose of 70 g/m2.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keragaman Lima Aksesi Jawer Kotok (Plectranthus scutellarioides (L.) R.Br.) Berdasarkan Morfologi dan Marka RAPD","authors":"Tias Arlianti, N. L. W. Meilawati, Rubi Heryanto, Susi Purwiyanti","doi":"10.15408/kauniyah.v16i2.1.25556","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.25556","url":null,"abstract":"AbstrakJawer kotok (Plectranthus scutellariodes (L) R.Br.) selain berfungsi sebagai tanaman obat, sering digunakan sebagai tanaman hias karena bentuk dan warna daunnya yang indah. Balai Penelitian Tanaman Rempah dan Obat (BALITTRO) memiliki lima aksesi Jawer kotok yang belum teridentifikasi keragaman genetiknya. Koleksi tersebut perlu dikarakterisasi untuk mendapatkan data morfologi tanaman yang dapat digunakan sebagai pembanding antartanaman secara cepat. Identifikasi keragaman genetik dapat menggunakan karakter morfologi ataupun marka molekuler seperti Random Amplified Polymorphic DNA (RAPD). Penelitian ini bertujuan menganalisis keragaman genetik lima aksesi Jawer kotok berdasarkan karakter morfologi dan marka RAPD. Bahan tanaman yang digunakan adalah lima aksesi Jawer kotok koleksi kebun percobaan Cimanggu BALITTRO, Bogor Jawa-Barat. Karakteristik morfologi yang diamati meliputi habitus, daun, batang, dan bunga. DNA diekstraksi dari daun segar dengan metode CTAB kemudian diamplifikasi menggunakan 20 primer RAPD. Analisa kekerabatan menggunakan metode Gower dan UPGMA dengan software PBSTAT. Hasil penelitian menunjukkan terdapat keragaman pada bentuk daun, bentuk ujung daun, warna daun, dan warna corak daun. Analisis molekuler menunjukkan primer OPK 20, OPB 8, OPD 11, dan OPB 13 merupakan primer dengan polimorfisme tertinggi. Kelima aksesi berdasarkan karakter morfologi dan RAPD memiliki hubungan kekerabatan antara 0,5–0,8. Hubungan kekerabatan terjauh adalah aksesi JK1dan JK5, sedangkan kekerabatan terdekat pada aksesi JK3 dan JK4. Karakter daun merupakan karakter dengan keragaman genetik yang tinggi, dan dapat dijadikan kriteria sebagai penciri utama.AbstractJawer kotok (Plectranthus scutellarioides (L) R.Br.) apart from functioning as a medicinal plant, is often used as an ornamental plant because of the beautiful shape and color of its leaves. The Research Institute for Spices and Medicinal Plants (BALITTRO) has five Jawer kotok accessions whose genetics have not been identified. These collections need to be characterized to obtain plant morphological data that can be used as a quick comparison between plants. Genetic identity can use morphological characters or molecular markers such as Random Appointed Polymorphic DNA (RAPD). This research aims to reveal the genetics of five Jawer kotok accessions based on morphological characters and RAPD markers. The plant materials used were five Jawer kotok accessions from the Cimanggu BALITTRO experimental garden, Bogor, West Java. Morphological characteristics observed include habitus, leaves, stems and flowers. DNA was extracted from fresh leaves using the CTAB method and then amplified using 20 RAPD primers. Relationship analysis uses the Gower and UPGMA methods with PBSTAT software. The research results showed that there was diversity in leaf shape, leaf tip shape, leaf color, and leaf pattern color. Molecular analysis showed that the primers OPK 20, OPB 8, OPD 11, and OPB 13 were the primers wit","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keragaman Kultivar Lokal Ubi Jalar (Ipomoea batatas (L.) Lam) di Kabupaten Kutai Timur-Kalimantan Timur, Indonesia","authors":"Maysavitrie Citra Wijayanti Kristianto, Medi Hendra, Linda Oktavianingsih","doi":"10.15408/kauniyah.v17i1.30539","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.30539","url":null,"abstract":"AbstrakUbi jalar (Ipomoea batatas (L.) Lam) merupakan salah satu tanaman pangan yang memiliki banyak manfaat di beberapa daerah di Indonesia termasuk di Kabupaten Kutai Timur. Informasi tentang keragaman morfologi kultivar lokal ubi jalar diperlukan untuk pemuliaan tanaman ke depannya. Penelitian dilakukan pada Maret-Juli 2021 untuk mengetahui keragaman dan hubungan kekerabatan kultivar lokal ubi jalar berdasarkan karakter morfologi. Kultivar lokal ubi jalar diambil secara purposive sampling melalui metode jelajah di 11 kecamatan, Kabupaten Kutai Timur. Karakterisasi morfologi menggunakan 32 karakter berdasarkan panduan International Board for Plant Genetic Resource (IBPGR). Analisis klaster untuk melihat hubungan kekerabatan antar kultivar menggunakan software MVSP versi 3.1. Hasil penelitian menunjukkan terdapat keragaman kultivar lokal ubi jalar yang ada di Kutai Timur berdasarkan karakter daun (bentuk, tipe cuping), umbi (bentuk, warna daging), bunga (bentuk dan warna). Hasil analisis klaster membentuk dua kelompok besar dari 23 aksesi kultivar lokal ubi jalar yang ditemukan dengan koefisien 9,671. Kelompok A dengan satu anggota, yaitu kultivar lokal 9 (Gei Meng Hom) yang terpisah dari kultivar lokal lainnya berdasarkan karakter umbi. Kultivar ini dibudidayakan oleh masyarakat asli Kalimantan (suku Dayak Wehea). Kelompok B terdiri dari 22 aksesi kultivar lokal yang dapat dikelompokkan berdasarkan karakter daun.AbstractSweet potato (Ipomoea batatas (L.) Lam) is a food crop that has many benefits in several regions in Indonesia, including East Kutai Regency. Information about the morphological diversity of local sweet potato cultivars is needed for future plant breeding. The research was conducted from March-July 2021 with the aim of determining the diversity and relationships of local sweet potato cultivars based on morphological characters. The collection of local sweet potato cultivars was carried out using purposive sampling using the roaming method in 11 sub-districts, East Kutai Regency. Morphological characterization uses 32 characters based on the International Board for Plant Genetic Resources (IBPGR) guidelines. Cluster analysis to see the kinship relationships formed between local sweet potato cultivars using MVSP software version 3.1. The results of the research show the diversity of local sweet potato cultivars in East Kutai based on leaf characters (shape, lobe type), tubers (shape, flesh color), flowers (shape and color), as well as forming two large groups based on the results of cluster analysis on 23 accessions which was found with a coefficient of 9.671. Group A with one member, namely local cultivar 9 (Gei Meng Hom) which is separated from other local cultivars based on tuber characteristics and is a local cultivar of sweet potato cultivated by the indigenous people of Kalimantan (Wehea Dayak tribe). Group B consists of 22 local cultivar accessions resulting from community cultivation which can be grouped based on leaf chara","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perkecambahan Biji Anggrek Grammatophyllum stapeliiflorum Pada Media MS dengan Penambahan BAP Secara In Vitro","authors":"Iga Permata Hany, Zozy Aneloi Noli, Suwirmen Suwirmen","doi":"10.15408/kauniyah.v17i1.27624","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.27624","url":null,"abstract":"AbstrakGrammatophyllum stapeliiflorum merupakan jenis anggrek epifit dengan pertumbuhan vegetatif dan generatif yang relatif lambat. Anggrek ini termasuk ke dalam kelompok CITES Apendiks II. Kultur in vitro merupakan usaha perbanyakan paling efektif untuk tanaman anggrek. Penggunaan media kultur dan Zat Pengatur Tumbuh (ZPT) yang tepat akan meningkatkan keberhasilan perkecambahan biji anggrek secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi media MS dan penambahan BAP terbaik terhadap perkecambahan anggrek G. stapeliiflorum secara in vitro. Penelitian ini menggunakan rancangan acak lengkap dengan 6 perlakuan dan 4 ulangan. Perlakuan berupa variasi konsentrasi media MS dan BAP, yaitu: MS penuh; MS ½ hara makro; MS ¼ hara makro; MS penuh + 1 ppm BAP; MS ½ hara makro + 1 ppm BAP; dan MS ¼ hara makro + 1 ppm BAP. Parameter yang diamati pada penelitian ini, yaitu waktu muncul protokorm dan persentase tahap perkecambahan biji. Data dianalisis menggunakan uji ANOVA dan uji lanjut Duncan New Multiple Range Test dengan taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP mampu mempercepat waktu muncul protokorm. Konsentrasi media MS ¼ hara makro + 1 ppm BAP merupakan konsentrasi terbaik untuk perkecambahan biji anggrek tahap 0 hingga tahap 3, sedangkan konsentrasi media MS ¼ hara makro merupakan konsentrasi terbaik untuk mencapai tahap 4 perkecambahan biji anggrek G. stapeliiflorum secara in vitro.AbstractGrammatophyllum stapeliiflorum is a type of epiphytic orchid with relatively slow vegetative and generative growth. This orchid is included in the CITES Appendix II group. In vitro culture is the most effective propagation method for orchid plants. The use of appropriate culture media and growth regulators will increase the success of orchid seed germination in vitro. This study aims to determine the effect of the best concentration of MS media and the addition of BAP on the germination of G. stapeliiflorum orchids in vitro. This study used a completely randomized design with 6 treatments and 4 replications. The treatments consisted of varying concentrations of MS and BAP media, namely: full MS; MS ½ macro nutrients; MS ¼ macro nutrients; full MS + 1 ppm BAP; MS ½ macro nutrients + 1 ppm BAP; and MS ¼ macro nutrients + 1 ppm BAP. The parameters observed in this study were the time when the protocorm appeared and the percentage of seed germination stages. Data were analyzed using the ANOVA test and the Duncan New Multiple Range Test with a level of 5%. The results of the study showed that administration of BAP was able to speed up the time when protocorm appeared. MS media concentration ¼ macro nutrients + 1 ppm BAP is the best concentration for stage 0 to stage 3 orchid seed germination, while MS media concentration ¼ macro nutrients is the best concentration for achieving stage 4 germination of G. stapeliiflorum orchid seeds in vitro.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Gen tufA Secara In Silico Untuk Primer Identifikasi Mikroalga Trebouxiophyceae","authors":"Megga Ratnasari Pikoli, Mahsa Nuraini Syahda, Festy Auliyaur Rahmah, Suharti Suharti","doi":"10.15408/kauniyah.v17i1.35458","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.35458","url":null,"abstract":"AbstrakPenelitian belakangan ini menunjukkan bahwa di antara mikroalga yang memiliki kandungan lipid tinggi untuk dimanfaatkan sebagai bahan baku biodiesel, termasuk ke dalam kelas Trebouxiophyceae.  Kesederhanaan sel dan bentuknya yang mudah berubah menjadikannya sulit diidentifikasi secara morfologis. Oleh karena itu, identifikasinya perlu didampingi dengan metode molekuler yang mengamplifikasi gen dengan polymerase chain reactions (PCR). Metode PCR membutuhkan primer yang membatasi area pada DNA yang akan diamplikasi. Gen yang berpotensi dijadikan penanda identifikasi adalah tufA karena memiliki urutan yang lestari. Penelitian ini bertujuan mengajukan primer berdasarkan gen tufA untuk identifikasi Trebouxiophyceae. Sekuen gen tufA dikumpulkan dari database, disejajarkan, dan diamati area yang lestari untuk diambil kandidat primer. Kemudian primer forward dan reverse dipasang-pasangkan sambil diperiksa untuk diperoleh kandidat dengan sifat-sifatnya terbaik. Ada 5 pasangan kandidat yang dihasilkan yang kemudian diperiksa spesifisitasnya dalam menjaring anggota genus dari Trebouxiophyceae, dan juga yang bukan Trebouxiophyceae (Chlorophyceae dan Ulvophyceae) sebagai pembanding. Pasangan primer yang terbaik diusulkan dari penelitian ini adalah pasangan primer tufA. Trebo1 yang terdiri atas primer forward 5’-GAAAGTGTTGCTGGTGATAATGTTGG-3’ dan reverse 5’-GGAGTATGTCGACCACCTTCTTC-3’ yang menjaring 75% Trebouxiophyceae di GenBank. Pasangan primer ini menjaring lebih banyak Trebouxiophyceae dibandingkan dengan primer tufA yang pernah dipublikasi, namun memerlukan optimasi kondisi PCR untuk meminimalkan potensi terjadinya struktur sekunder. Dengan demikian, area lestari pada gen tufA berpotensi dijadikan primer untuk identifikasi Trebouxiophyceae.AbstractRecent research showed that microalgae having high lipid content to be used as raw materials for biodiesel belong to the class Trebouxiophyceae.  The simplicity of the cell and its easily changing shape make it difficult to identify morphologically. Therefore, its identification needs to be accompanied by molecular methods that amplify genes with polymerase chain reactions (PCR). The gene that could potentially be used as an identification marker is tufA because it has a conserved sequence. This study aims to propose a primer pair based on the tufA gene for the identification of Trebouxiophyceae. The sequences of the tufA gene were collected from a database of Trebouxiophyceae, aligned, and observed in conserved areas for primer candidates. Then the primary forward and reverse are mounted while checking for the candidate with the best properties. Five candidate pairs were produced, which were then tested for their specificity to bring in members of the Trebouxiophyceae, as well as non-Trebouxiophyceae (Chlorophyceae and Ulvophyceae) as comparisons. The best proposed primary pairs from this study were the primer pair tufA.Trebo1 which consists of the forward 5’-GAAAGTGTTGCTGGTGATAATGTTGG-3’ and the reverse ","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139176229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INDEX AL-KAUNIYAH: JURNAL BIOLOGI VOL. 17 NO. 1 APRIL 2024","authors":"Index Index","doi":"10.15408/kauniyah.v17i1.36731","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.36731","url":null,"abstract":"","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}