E. Rosyidatun, Latifah Azzahra, F. Wijayanti, Walid Rumblat, M. Mardiansyah
{"title":"Vegetasi Riparian dan Komunitas Makrozoobentos di Situ Gintung Sebagai Pendukung Konservasi Air Kawasan Kampus UIN Syarif Hidayatullah Jakarta","authors":"E. Rosyidatun, Latifah Azzahra, F. Wijayanti, Walid Rumblat, M. Mardiansyah","doi":"10.15408/kauniyah.v17i1.35756","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.35756","url":null,"abstract":"AbstrakSitu Gintung merupakan ekosistem perairan yang sangat penting dalam konservasi perairan di Kawasan Kampus 2 UIN Syarif Hidayatullah Jakarta. Riparian merupakan vegetasi yang tumbuh di tepi perairan dan berpotensi sebagai habitat dan sumber makanan bagi ekosistem perairan. Penelitian ini bertujuan untuk mengetahui struktur vegetasi riparian dan pengaruhnya terhadap keanekaragaman makrozoobentos di Situ Gintung. Titik pengambilan sampel terdiri dari 8 stasiun dengan 3 kali pengulangan. Analisis hubungan vegetasi riparian dengan makrozoobentos diuji dengan regresi linier sederhana dan korelasi Pearson. Hasil penelitian menunjukkan bahwa vegetasi riparian yang terdapat pada 8 stasiun terdiri dari 2 kelas (Magnoliopsida dan Liliopsida), 11 spesies dengan nilai indeks keanekaragaman (H') sebesar 1,517. Makrozoobentos yang ditemukan terdiri dari 2 kelas (Gastropoda dan Crustacea) 7 spesies dengan nilai indeks keanekaragaman (H') sebesar 1,112. Hasil analisis regresi menunjukkan adanya korelasi antara vegetasi riparian dengan kepadatan makrozoobentos yang tergolong sempurna (0,972). Hal ini menunjukkan bahwa vegetasi riparian di Situ Gintung berkorelasi positif dengan keanekaragaman makrozoobentos.AbstractSitu Gintung is an aquatic ecosystem that is very important in water conservation in the Campus 2 Area of UIN Syarif Hidayatullah Jakarta. Riparian is vegetation that grows at the edge of water and has the potential to serve as habitat and food source for aquatic ecosystems. This research aims to determine the structure of riparian vegetation and its influence on macrozoobenthos diversity in Situ Gintung. The sampling point consists of 8 stations with 3 repetitions. Analysis of the relationship between riparian vegetation and macrozoobenthos was tested using simple linear regression and Pearson correlation. The research results showed that the riparian vegetation was found at 8 stations consisting of 2 classes (Magnoliopsida and Liliopsida), 11 species with a diversity index (H') value of 1.517. The macrozoobenthos found consisted of 2 classes (Gastropoda and Crustacea) 7 species with a diversity index (H') value of 1.112. The results of the regression analysis showed that there was a correlation between riparian vegetation and macrozoobenthos density which was classified as perfect (0.972). This shows that riparian vegetation in Situ Gintung is positively correlated with macrozoobenthos.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"154 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efektivitas Pertumbuhan dan Hasil Tanam Beberapa Kultivar Kedelai (Glycine max (L) Merr.) dengan Pemberian Polietilena Glikol (PEG) Untuk Simulasi Cekaman Kekeringan","authors":"Winda Anindya, Dian Palupi, Iman Budisantoso","doi":"10.15408/kauniyah.v17i1.29345","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.29345","url":null,"abstract":"AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh interaksi varietas kedelai dengan konsentrasi Polietilen glikol (PEG) yang diberikan terhadap pertumbuhan serta hasil tanam dan menentukan varietas kedelai yang toleran terhadap cekaman kekeringan. Penelitian dilakukan dengan menggunakan metode Rancangan Acak Kelompok (RAK) faktorial yang terdiri dari 2 faktor. Faktor pertama adalah varietas kedelai, yaitu Gepak Kuning, Argomulyo, dan Anjasmoro, sedangkan faktor kedua adalah konsentrasi PEG yang diberikan, yaitu 0% (kontrol), 5%, 10%, dan 15%. Pengamatan dilakukan secara destruktif pada hari ke-20, 40, 60 setelah tanam dan saat panen dengan ulangan dilakukan sebanyak tiga kali, sehingga terdapat 36 unit percobaan. Parameter yang diamati meliputi bobot basah, bobot kering, luas daun, laju asimilasi bersih, dan bobot biji pertanaman. Data yang diperoleh dianalisis menggunakan ANOVA pada taraf 5% dan dilakukan uji Honestly Significance Different. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara varietas kedelai dan konsentrasi PEG yang diberikan. Pada variabel yang diamati varietas Argomulyo memiliki respon fisiologi lebih baik dengan nilai rata-rata bobot basah, bobot kering, luas daun, laju asimilasi bersih, dan bobot biji pertanaman tertinggi dibandingkan Anjasmoro dan Gepak Kuning pada seluruh konsentrasi PEG. AbstractThe first aim of this research was to determine the effect of the interaction of soybean varieties with the concentration of Polyethylene glycol (PEG) given on plant growth and yield, and secondly to determine soybean varieties that are tolerant to drought stress. The research was conducted using the factorial Completely Randomized Design (RAK) method consisting of 2 factors. The first factor is the soybean variety, namely Gepak yellow, Argomulyo, and Anjasmoro, while the second factor is the PEG concentration given, namely 0% (control), 5%, 10%, and 15%. Observations were carried out destructively on days 20, 40, 60 after planting and at harvest with repetitions carried out three times, so there were 36 experimental units. The parameters observed included wet weight, dry weight, leaf area, net assimilation rate, and seed weight planted. The data obtained were analyzed using ANOVA at the 5% level and an Honestly Significance Different test was carried out. The results showed that there was no interaction between soybean varieties and the PEG concentration provided. In the variables observed, the Argomulyo variety had a better physiological response with the highest average value of wet weight, dry weight, leaf area, net assimilation rate, and seed weight compared to Anjasmoro and Gepak Kuning at all PEG concentrations.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"86 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pertumbuhan dan Produksi Tanaman Kale (Brassica oleracea L. var. acephala) Pada Sistem Hidroponik Deep Flow Technique dengan Penambahan Pupuk Organik Cair","authors":"Dasumiati Dasumiati, Mutiara Marhaban Siregar, Ardian Khairiah, Junaidi Junaidi","doi":"10.15408/kauniyah.v17i1.35563","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.35563","url":null,"abstract":"AbstrakKale merupakan tanaman hortikultura yang kaya antioksidan, karotenoid, dan antosianin. Kale dapat dibudidayakan secara hidroponik dengan menggunakan AB mix sebagai pupuk, namun nutrisinya sering mengendap di dasar bak sulit terserap oleh akar. Penambahan Pupuk Organik Cair (POC) yang disemprotkan ke daun diharapkan dapat menjadi nutrisi tambahan bagi tanaman. Penelitian ini bertujuan untuk menganalisis respon pertumbuhan dan produksi tanaman kale terhadap penambahan POC pada sistem hidroponik Deep Flow Technique (DFT). Penelitian menggunakan Rancangan Acak Kelompok dengan 5 perlakuan, yaitu dosis POC (0; 10; 20; 30; dan 40 mL/L). POC disemprotkan ke daun setiap minggu sejak hari ke-0 sampai 35 hari setelah tanaman. Analisis data menggunakan sidik ragam (ANOVA) dan uji lanjut DMRT pada taraf 5%. Penambahan POC berpengaruh nyata pada pertumbuhan tanaman kale (P <0,05), yaitu POC konsentrasi 10 mL/L dapat meningkatkan panjang akar tanaman (41,25 cm) dan berpengaruh tidak nyata terhadap parameter lainnya seperti tinggi tanaman, jumlah daun, diameter batang, berat basah, dan kering tanaman serta berat layak konsumsi. Namun, tanaman kale yang disemprot POC memiliki nilai rata-rata semua parameter lebih tinggi dari kontrol (0 mL/L). Perlakuan POC 10 mL/L memiliki rata-rata parameter pertumbuhan dan produksi lebih tinggi dari yang lainnya, sehingga berpotensi meningkatkan pertumbuhan dan produksi tanaman kale pada sistem hidroponik DFT.AbstractKale is a horticultural plant that is rich in antioxidants, carotenoids and anthocyanins. Kale can be cultivated hydroponically using AB mix as fertilizer, but the nutrients often settle at the bottom of the tub and are difficult for the roots to absorb. The addition of Liquid Organic Fertilizer (POC) which is sprayed through the leaves is expected to provide additional nutrition for plants. This research aims to analyze the growth and production response of kale plants to the addition of POC in the Deep Flow Technique (DFT) hydroponic system. The study used a randomized block design with 5 treatments, namely POC doses (0; 10; 20; 30; and 40 mL/L). POC is sprayed on the leaves every week from day 0 to 35 days after planting. Data analysis used analysis of variance (ANOVA) and further DMRT test at the 5% level. The addition of POC has a significant effect on the growth of kale plants (P <0.05), namely POC concentration of 10 mL/L can increase plant root length (41.25 cm) and has no significant effect on other parameters such as plant height, number of leaves, stem diameter. , wet and dry weight of the plant and weight suitable for consumption. However, kale plants sprayed with POC had an average value of all parameters higher than the control (0 mL/L). The 10 mL/L POC treatment has higher average growth and production parameters than the others, so it has the potential to increase the growth and production of kale plants in the DFT hydroponic system.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"128 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laurentia Henrieta Permita Sari Purba, Jeremia Frandy Apitalau, Guruh Prihatmo
{"title":"Komposisi Pakan Lutung Jawa (Trachypithecus auratus E. Geoffroy Saint-Hilaire, 1812) di Kebun Binatang Gembira Loka, Yogyakarta","authors":"Laurentia Henrieta Permita Sari Purba, Jeremia Frandy Apitalau, Guruh Prihatmo","doi":"10.15408/kauniyah.v17i1.27539","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.27539","url":null,"abstract":"AbstrakKemampuan untuk menemukan, memanen, dan memproses makanan sangat penting untuk kelangsungan hidup hewan. Pemilihan jenis pakan pada hewan sangat bergantung pada ketersediaan makanan di habitatnya dan adaptasi morfologi khususnya organ pencernaan hewan. Trachypithecus auratus adalah primata endemik Pulau Jawa, Bali, dan Lombok yang terdaftar sebagai satwa rentan oleh IUCN (vulnerable) dan CITES (Appendix II). Upaya konservasi Lutung Jawa antara lain dengan sistem ex-situ di Kebun Binatang Gembira Loka, Yogyakarta. Pemberian jenis pakan yang disesuaikan dengan pakan alami Lutung Jawa menjadi salah satu upaya untuk mencapai kesejahteraan hewan konservasi. Oleh karena itu, penelitian ini dilakukan untuk mengamati komposisi pakan populasi Lutung Jawa di Kebun Binatang Gembira Loka dengan total waktu 480 jam melalui metode scan sampling. Komposisi pakan Lutung Jawa yang diberikan oleh Kebun Binatang Gembira Loka terdiri adalah daun (7%), buah dan sayur (92%), dan suplemen (4%), Jenis pakan yang paling banyak dikonsumsi oleh Lutung Jawa di Kebun Binatang Gembira Loka adalah buah dan sayur. Hal ini menunjukkan perilaku makan Lutung Jawa di Kebun Binatang Gembira Loka berbeda dengan perilaku makan alaminya, yaitu sebagai pemakan daun (folivor) sehingga diperlukan penyesuaian komposisi pakan lutung Jawa di Kebun Binatang Gembira Loka, Yogyakarta.AbstractThe ability to find, harvest, and process food is critical to animal survival. The choice of food type for animals is very dependent on the availability of food in their habitat and morphological adaptations, especially the animal's digestive organs. Trachypithecus auratus is a primate endemic to the islands of Java, Bali and Lombok which is listed as vulnerable by IUCN (vulnerable) and CITES (Appendix II). Javanese langur conservation efforts include an ex-situ system at the Gembira Loka Zoo, Yogyakarta. Providing types of feed that are compatible with the natural diet of Javan langurs is one of the efforts to achieve the welfare of conservation animals. Therefore, this research was conducted to observe the food composition of the Javan langur population at Gembira Loka Zoo for a total of 480 hours using the scan sampling method. The composition of the Javanese langur feed provided by the Gembira Loka Zoo consists of leaves (7%), fruit and vegetables (92%), and supplements (4%), where fruit and vegetable feed has the highest percentage of consumption by each individual. Apart from that, the group of food consumed was compared with wild populations from the results of research that has been carried out, where Javan langurs at the Gembira Loka Zoo consume more fruit and vegetables. This shows that the eating behavior of Javan langurs at Gembira Loka Zoo is different from their natural eating behavior, namely leaf eating (folivor), so it is necessary to adjust the food composition of Javan langurs at Gembira Loka Zoo, Yogyakarta.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"84 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pengaruh Pemberian Jus Pare (Momordica charantia L.) Terhadap Kualitas Sperma dan Histologi Testis Tikus Wistar","authors":"Haris Setiawan, Roby Ahmad Subagja","doi":"10.15408/kauniyah.v16i2.1.23216","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.23216","url":null,"abstract":"AbstrakBuah pare merupakan salah satu kandidat agen kontrasepsi karena memiliki beberapa senyawa seperti flavonoid, triterpenoid, steroid, charantin, dan momordicin yang memiliki peran sebagai agen antispermatogenik. Penelitian bertujuan untuk mengetahui pengaruh pemberian jus pare (Momordica charantia L.) terhadap kualitas sperma dan histologi testis tikus Wistar (Rattus norvegicus Berkenhout, 1769). Penelitian menggunakan 20 ekor tikus Wistar jantan, dibagi menjadi 4 perlakuan yang terdiri dari kelompok pemberian akuades (K), kelompok pemberian jus pare konsentrasi 25% (P1), 50% (P2), dan 75% (P3) yang dilakukan selama 49 hari menggunakan sonde lambung 1 mL. Pada hari ke-50 tikus dibedah untuk diambil cauda epididymis dan testis. Cauda epididymis dilarutkan ke dalam Phospate Buffer Saline (PBS) untuk pengamatan kualitas sperma yang terdiri dari motilitas, jumlah, viabilitas, dan morfologi sperma. Testis dibuat sediaan histologi dengan metode parafin (pewarnaan Hematoxylin-Eosin). Seluruh parameter dianalisis menggunakan uji One-Way Anova dan dilanjutkan Duncan test (P <0,05). Hasil menunjukkan terdapat penurunan motilitas, viabilitas, dan jumlah sperma pada konsentrasi 50% dibandingkan dengan kontrol (P <0,05), namun tidak terdapat perbedaan pada morfologi sperma (P >0,05). Terdapat penurunan jumlah sel spermatogonium, spermatosit, dan spermatozoa pada konsentrasi 25% dibandikan dengan kontrol (P <0,05), namun tidak terlihat penurunan pada sel spermatid dan index spermatogenesis (P >0,05). Jus pare dapat menurunkan sebagian besar parameter kualitas sperma sehingga berpotensi sebagai antispermatogenik.AbstractBitter melon fruit is one of the candidates for contraceptive agents because it contains several compounds such as flavonoids, triterpenoids, steroids, charantin, and momordicin which have a role as antispermatogenic agents. The research aims to determine the effect of giving bitter melon juice (Momordica charantia L.) on sperm quality and testicular histology of Wistar rats (Rattus norvegicus Berkenhout, 1769). The study used 20 male Wistar rats, divided into 4 treatments consisting of groups given distilled water (K), groups given bitter melon juice with concentrations of 25% (P1), 50% (P2), and 75% (P3) which were carried out for 49 days. using a 1 mL gastric probe. On the 50th day, mice were dissected to remove the cauda epididymis and testes. The cauda epididymis is dissolved in Phosphate Buffer Saline (PBS) to measure sperm quality consisting of sperm motility, number, viability and morphology. Testes were made into histological preparations using the paraffin method (Hematoxylin-Eosin staining). All parameters were analyzed using the One-Way Anova test and continued with the Duncan test (P<0.05). The results showed that there was a decrease in sperm motility, viability and number at a concentration of 50% compared to the control (P<0.05), but there was no difference in sperm morphology (P>0.05). There was a decrease in the number of ","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"33 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identifikasi Metabolit Sekunder dan Aktivitas Antioksidan Karang Lunak Nephthea sp. Hasil Transplantasi Secara In Situ dan Ex Situ","authors":"Fahri Fahrudin, Dinda Rama Haribowo, Rahmi Karmila, Danang Aji Pangestu, Fathin Hamida","doi":"10.15408/kauniyah.v17i1.35781","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.35781","url":null,"abstract":"AbstrakKarang lunak (Nephthea sp.) menghasilkan metabolit sekunder di habitat aslinya dan dapat dikembangkan menjadi marine natural product (MNP). Nephthea sp. hasil transplantasi diharapkan juga menghasilkan metabolit sekunder yang sama. Tujuan penelitian ini adalah melakukan identifikasi metabolit sekunder secara kualitatif dan kuantitatif pada Nephthea sp. hasil transplantasi serta menguji aktivitas antioksidannya. Sampel Nephthea sp. berasal dari Taman Nasional Ujung Kulon (in situ) dan dari akuarium sebagai sampel transplantasi ex situ. Kedua sampel diekstraksi dengan maserasi menggunakan etanol 70% dengan perbandingan 1:3 (w/v). Identifikasi metabolit sekunder dilakukan secara kualitatif dan metode Gas Chromatography-Mass Spectrometry (GC-MS) untuk identifikasi kuantitatif. Aktivitas antioksidan menggunakan uji DPPH (2,2-difenil-1-1 pikrilhidrazil) dengan lima konsentrasi dari setiap sampel (10 ppm, 50 ppm, 100 ppm, 150 ppm, dan 200 ppm). Nephthea sp. hasil transplantasi memiliki metabolit sekunder golongan alkaloid, tanin, dan steroid. Hasil uji GC-MS menunjukkan terdapat enam jenis senyawa aktif golongan benzene dan fatty acid. Aktivitas antioksidan diperoleh 34,3–73,1% (in situ) dan 33,2–72,3% (ex situ) serta berbeda nyata (P <0,05) pada setiap konsentrasi dari dua sampel. Peningkatan aktivitas antioksidan Nephthea sp. tidak berbeda signifikan (P >0,05) pada perbedaan lokasi transplantasi (in situ dan ex situ). Nephthea sp. hasil transplantasi mengandung enam jenis senyawa metabolit sekunder dan memiliki aktivitas antioksidan.AbstractSoft corals (Nephthea sp.) produce secondary metabolites in their habitat and can be used as marine natural products (MNP). However, Nephthea sp. transplantation not done identified for secondary metabolites. The aim research to identify secondary metabolites and antioxidant activity assay in Nephthea sp. transplant. Samples of Nephthea sp. used from the transplant area of Ujung Kulon National Park (in situ) and from the aquarium as ex situ transplant samples. Both samples extracted by maceration with 70% ethanol (1:3, w/v). Identification of secondary metabolites was carried out qualitatively and using the GC-MS method for quantitative. Antioxidant activity using the DPPH (2,2-diphenyl-1-1 picrylhydrazyl) assay. with concentrations of each sample (10 ppm, 50 ppm, 100 ppm, 150 ppm, and 200 ppm). Nephthea sp. proven to have secondary metabolites from the alkaloids, tannins and steroids. The result of GC-MS showed that six types of active compounds from the benzene and fatty acid groups. Antioxidant activity obtained was 34.3–73.1% (in situ) dan 33.2–72.3% (ex situ) and was significantly different (P <0.05) at each concentration of the two samples. Antioxidant activity at different transplant locations (in situ and ex situ) in Nephthea sp. did not a significant (P >0.05). Thus, Nephthea sp. transplant were proven to contain six types of secondary metabolite compounds and has antioxidant activity.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139176227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kartina Kartina, Ramli Ramli, Awaludin Awaludin, N. Nurasmi
{"title":"Pengaruh Penambahan Ekstrak Daun Katuk (Sauropus androgynus) Pada Pakan Terhadap Performa Ikan Bandeng (Chanos chanos)","authors":"Kartina Kartina, Ramli Ramli, Awaludin Awaludin, N. Nurasmi","doi":"10.15408/kauniyah.v16i2.1.27374","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.27374","url":null,"abstract":"AbstrakKatuk (Sauropus androgynus) merupakan tumbuhan herbal yang mengandung protein dan asam lemak yang banyak dimanfaatkan untuk meningkatkan pertumbuhan hewan. Penelitian ini bertujuan untuk menentukan dosis ekstrak daun katuk pada pakan yang dapat meningkatkan pertumbuhan dan survival rate benih ikan bandeng. Benih ikan bandeng yang digunakan berukuran 1,8–1,9 cm dan dipelihara pada akuarium bervolume 15 L dengan padat tebar 15 ekor dalam setiap akuarium. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Perlakuan berupa penambahan ekstrak daun katuk pada pakan dengan dosis 0 g/kg (kontrol atau P1), 37,5 g/kg (P2), 75 g/kg (P3), 150 g/kg (P4), dan 300 g/kg (P5) pakan. Penambahan ekstrak daun katuk pada pakan berpengaruh nyata terhadap pertumbuhan mutlak dan Survival Growth Rate (SGR) benih ikan bandeng (P<0,05), dan tidak berpengaruh nyata terhadap survival rate (SR) (P>0,05). Penambahan ekstrak daun katuk sebesar 150 g/kg pakan (P4) menunjukan nilai terbaik pada pertumbuhan mutlak dan SGR benih. Nilai SR tertinggi ditunjukan pada penambahan ekstrak sebesar 300 g/kg pakan (P5) yaitu mencapai 80%. Data pengukuran kualitas air dalam nilai yang baik yaitu suhu 24–30 °C; pH 6–8; salinitas 24–30 ppt; dissolved oxygen (DO) 4,27–5,98 mg/L; dan amoniak 0,07–0,11 mg/L.AbstractKatuk (Sauropus androgynus) is a herbal plant that contains protein and fatty acids which are widely used to increase animal growth. This research aims to determine the dose of katuk leaf extract in feed that can increase the growth and survival rate of milkfish fry. The milkfish seeds used were 1.8–1.9 cm in size and were maintained in 15 L volume aquariums with a stocking density of 15 fish in each aquarium. This research is an experimental study using a completely randomized design (CRD) with 5 treatments and 3 replications. The treatment consisted of adding katuk leaf extract to the feed, namely control P1 0 g/kg, P2 37.5 g/kg, P3 75 g/kg, P4 150 g/kg, and P5 300 g/kg feed. The results of statistical analysis showed that the addition of katuk leaf extract to feed had a significant effect on the absolute growth and Survival Growth Rate (SGR) of milkfish seeds, while the survival rate (SR) showed values that were not significantly different. The addition of katuk leaf extract at 150 g/kg feed (P4) showed the best value for absolute growth and SGR of seeds. The highest SR value was shown when the extract was added at 300 g/kg feed (P5), reaching 80%. The water quality measurement data is in good value, namely a temperature of 24–30 °C; pH 6–8; salinity 24–30 ppt; dissolved oxygen (DO) 4.27–5.98 mg/L; and ammonia 0.07–0.11 mg/L.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"54 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Wartono, W. Wawan, D. Susilowati, Sukamto Sukamto, Jajang Kosasih
{"title":"Colletotrichum spp. Penyebab Penyakit Antraknosa Pada Tanaman Cabai Merah (Capsicum annuum) di Ciapus, Bogor, Jawa Barat","authors":"W. Wartono, W. Wawan, D. Susilowati, Sukamto Sukamto, Jajang Kosasih","doi":"10.15408/kauniyah.v16i2.1.27460","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.27460","url":null,"abstract":"AbstrakAntraknosa merupakan penyakit utama penyebab rendahnya produksi cabai. Penelitian ini bertujuan mengidentifikasi Colletotrichum penyebab penyakit antraknosa pada tanaman cabai di Ciapus, Bogor, Jawa Barat. Identifikasi morfologi dilakukan dengan mengamati pertumbuhan, warna, dan bentuk koloni miselium. Karakter mikromorfologi dievaluasi dengan mengamati bentuk, panjang, dan lebar konidia. Identifikasi secara molekuler dengan mengamplifikasi DNA cendawan menggunakan primer ITS5F dan ITS4R, sekuensing, analisis BLAST, serta analisis filogeni. Uji patogenititas dilakukan dengan menginokulasi buah cabai menggunakan 20 mL suspensi inokulum (105 konidia/mL), dilanjutkan dengan mengamati luas gejala hingga 6 hari setelah inokulasi (hsi). Semua isolat menunjukkan koloni berbentuk bulat, berwarna putih dan keabuan dengan kecepatan tumbuh koloni berkisar antara 2,0–6,4 mm/hari. Keempat isolat memproduksi konidia berbentuk silindris, berujung tumpul dengan panjang dan lebar yang beragam. Hasil analisis molekuler menunjukkan bahwa isolat CSColl-2, CSColl-3, dan CSColl-4 teridentifikasi sebagai Colletotrichum gloeosporioides, sedangkan CSColl-7 sebagai C. brevisporum. Semua isolat patogenik terhadap buah cabai yang diuji menyebabkan luas gejala berkisar antara 3,6–10,0 mm2. Informasi spesies Colletotrichum spp. dari penelitian ini bermanfaat untuk menentukan tindakan pengendalian yang tepat dan prapemuliaan perakitan cabai tahan penyakit antraknosa.AbstractAnthracnose is the main disease causing low chili production. This research aims to identify Colletotrichum which causes anthracnose disease in chili plants in Ciapus, Bogor, West Java. Morphological identification is carried out by observing the growth, color and shape of the mycelium colonies. Micromorphological characters were evaluated by observing the shape, length and width of the conidia. Molecular identification by amplifying fungus DNA using primers ITS5F and ITS4R, sequencing, BLAST analysis, and phylogeny analysis. The pathogenicity test was carried out by inoculating chili fruit using 20 μL of inoculum suspension (105 conidia/mL), followed by observing the extent of symptoms up to 6 days after inoculation (hsi). All isolates showed round, white and gray colonies with colony growth speeds ranging from 2.0–6.4 mm/day. The four isolates produced cylindrical, blunt-tipped conidia with varying lengths and widths. The results of molecular analysis showed that isolates CSColl-2, CSColl-3, and CSColl-4 were identified as Colletotrichum gloeosporioides, while CSColl-7 was C. brevisporum. All pathogenic isolates tested for chili fruit caused symptom areas ranging from 3.6–10.0 mm2. Species information Colletotrichum spp. This research is useful for determining appropriate control measures and pre-breeding of anthracnose-resistant chili plants.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":"22 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}