Al-Kauniyah: Jurnal Biologi最新文献

{"title":"Uji Potensi Kurkumin Terhadap Perbaikan Disfungsi Testis Tikus (Rattus norvegicus) Jantan Setelah Paparan Ekstrak Etanol Daun Mimba","authors":"Agung Janika Sitasiwi, Tyas Rini Saraswati, Silvana Tana, Sri Isdadiyanto, Siti Muflichatun Mardiati","doi":"10.15408/kauniyah.v17i1.30801","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.30801","url":null,"abstract":"AbstrakMimba merupakan tanaman yang terbukti memiliki efek antifertlitas. Efek senyawa antifertilitas dapat menyebabkan disfungsi organ reproduksi yang menyebabkan perubahan ukuran organ atau gangguan sintesis hormonal. Kurkumin merupakan sediaan yang dapat meningkatkan sintesis hormon testoteron pada tikus, tetapi belum digunakan untuk meningkatkan potensi reproduksi pada hewan yang telah terpapar senyawa antifertilitas. Penelitian ini menguji potensi kurkumin dalam memperbaiki disfungsi testis yang disebabkan oleh paparan ekstrak etanol daun mimba. Tikus Wistar (R. norvegicus) jantan dewasa digunakan sebagai hewan uji, dikelompokkan menjadi empat kelompok, yaitu P0 (kontrol, hewan uji diberi akuades), P1, P2, dan P3 (kelompok perlakuan, diberi sediaan ekstrak etanol daun mimba masing-masing dengan dosis 60, 80, dan 100 mg/kgBB/hari). Pemberian bahan uji dilakukan secara oral selama 14 hari, dilanjutkan dengan pemberian kurkumin dengan dosis 1,35 mg/200gBB/hari selama 14 hari. Pengambilan sampel testis dan darah untuk pengukuran testoteron dilakukan setelah paparan bahan uji 1 selesai (hari ke-15) dan setelah paparan bahan uji 2 (hari ke-29). Variabel penelitian adalah indeks gonadosomatik, volume testis dan kadar testoteron. Hasil penelitian menunjukkan bahwa kurkumin mampu meningkatkan kandungan hormon testoteron serta meningkatkan indeks gonadosomatik hewan uji sehingga dapat disimpulkan bahwa kurkumin berpotensi memperbaiki disfungsi testis akibat paparan senyawa antifertilitas.AbstractNeem is a plant that has been proven to have antifertility effects. The effects of antifertility compounds can cause reproductive organ dysfunction which causes changes in organ size or disruption of hormonal synthesis. Curcumin is a preparation that can increase testosterone hormone synthesis in mice, but has not been used to increase reproductive potential in animals that have been exposed to antifertility compounds. This research was conducted to test the potential of curcumin in improving testicular dysfunction caused by exposure to ethanol extract of neem leaves. Adult male Wistar rats (R. norvegicus) were used as test animals, identified into four groups, namely P0 (control, test animals given distilled water), P1, P2, and P3 (treatment groups, given ethanol extract of neem leaves each with doses of 60, 80, and 100 mg/kgBW/day). The test material was administered orally for 14 days, followed by administration of curcumin at a dose of 1.35 mg/200gBW/day for 14 days. Testicular and blood samples were taken for testosterone measurement after exposure to test substance 1 was completed (day 15) and after exposure to test substance 2 (day 29). The research variables were gonadosomatic index, testicular volume and testosterone levels. The results of the study showed that curcumin was able to increase testosterone hormone content and increase the gonadosomatic index of test animals, so it can be concluded that curcumin has the potential to recover testicular dys","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studi Etnobotani Pemanfaatan Zingibereaceae Oleh Masyarakat Melayu di Pulau Rupat, Kabupaten Bengkalis, Riau","authors":"N. Hafizah, Fitmawati Fitmawati","doi":"10.15408/kauniyah.v16i2.1.24374","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.24374","url":null,"abstract":"AbstrakKearifan lokal etnis Melayu Rupat dalam memanfaatkan tumbuhan obat sangat khas, namun pengetahuan lokal tersebut belum pernah diungkap dan didokumentasikan dengan baik secara ilmiah. Penelitian ini bertujuan untuk mengidentifikasi jenis, manfaat, dan cara pemanfaatan tumbuhan Zingiberaceae oleh masyarakat Melayu di Pulau Rupat. Pengumpulan data dilakukan melalui wawancara secara terbuka dan semi terstruktur terhadap 15 praktisi obat tradisional. Data kualitatif dianalisis secara deskriptif dan data kuantitatif dianalisis dengan menghitung Use Value (UV), Index of Cultural Significance (ICS), Informant Consensus Factor (ICF), dan Fidelity Level (FL). Sebanyak 14 spesies dan lima genus famili Zingiberaceae yang dimanfaatkan masyarakat Melayu Rupat untuk mengobati sekitar 33 jenis penyakit yang dikelompokkan menjadi enam kategori di antaranya masalah kesehatan wanita, sakit kepala, masalah pencernaan, masalah otot, dan sendi, masalah sirkulasi serta masalah pada kulit. Hasil analisis diketahui bahwa tumbuhan Curcuma longa, Curcuma xanthorrhiza, Kaempferia galanga, dan Zingiber officinale var. rubrum memiliki nilai UV tertinggi yang mengindikasikan bahwa tumbuhan tersebut dianggap penting karena diketahui oleh semua informan. Keempat tumbuhan tersebut juga memiliki nilai ICS tertinggi karena memiliki khasiat paling banyak sebagai bahan obat. Terdapat 11 jenis penyakit yang memiliki nilai ICF tertinggi, yakni tingkat kesepakatan masyarakat dalam menggunakan suatu jenis tumbuhan untuk mengobati penyakit tersebut sangat tinggi. Nilai FL tertinggi mencapai 100% diperoleh pada tumbuhan Curcuma longa, Curcuma xanthorrhiza, dan Zingiber officinale var. Rubrum, artinya masyarakat memiliki pengetahuan yang sama dalam memanfaatkan ketiga jenis tumbuhan tersebut.Kata Kunci: Bengkalis; Etnobotany; Melayu; Pulau Rupat; ZingiberaceaeAbstractThe local wisdom of the Rupat Malay ethnic group in using medicinal plants is very unique, however, this local knowledge has never been revealed and documented properly scientifically. This research aims to identify the types, benefits and ways of using Zingiberaceae plants by the Malay community on Rupat Island. Data collection was carried out through open and semi-structured interviews with 15 traditional medicine practitioners. Qualitative data was analyzed descriptively and quantitative data was analyzed by calculating Use Value (UV), Index of Cultural Significance (ICS), Informant Consensus Factor (ICF), and Fidelity Level (FL). There are 14 species and five genera of the Zingiberaceae family that are used by the Rupat Malay community to treat approximately 33 types of diseases which are grouped into six categories including women's health problems, headaches, digestive problems, muscle and joint problems, circulation problems and skin problems. The plants Curcuma longa, Curcuma xanthorrhiza, Kaempferia galanga, and Zingiber officinale var. rubrum has the highest UV value which indicates that this plant is considere","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas Inhibitor Lipase Ekstrak Daun Mangga Arum Manis dan Mangga Kweni Secara In Vitro","authors":"Oktira Roka Aji, Nora Bastiani, Merlia Rahma Tari, Diah Asta Putri","doi":"10.15408/kauniyah.v16i2.1.18776","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.18776","url":null,"abstract":"AbstrakObesitas disebabkan adanya kelebihan kalori dalam tubuh. Penurunan obesitas dapat dilakukan dengan cara menghambat penyerapan lemak sebagai sumber utama kelebihan kalori. Salah satu cara yang dapat dilakukan untuk menghambat penyerapan lemak adalah dengan menghambat aktivitas lipase pankreas. Penelitian ini dilakukan untuk menentukan aktivitas inhibitor lipase ekstrak metanol daun mangga arum manis (Mangifera indica L. cv Arum manis) dan kweni (Mangifera odorata Griff.). Tahapan penelitian diawali dengan ekstraksi menggunakan metode maserasi lalu diikuti dengan uji kadar total fenol, kadar flavonoid, dan uji aktivitas inhibitor lipase ekstrak daun mangga arum manis serta kweni. Hasil uji kadar total fenol menunjukkan total fenol pada ekstrak daun mangga arum manis dan kweni berturut-turut yaitu 246,94 mg GAE/g dan 176,11 mg GAE/g. Kadar flavonoid daun mangga arum manis, yaitu 129,95 mg.QE/g, sedangkan daun mangga kweni yaitu 26,50 mg.QE/g. Masing-masing ekstrak diuji kemampuannya dalam menghambat lipase pankreas secara in vitro. Nilai IC50 ekstrak daun mangga arum manis dan kweni, yaitu 61,55 µg/mL dan 79,98 µg/mL. Nilai tersebut lebih tinggi dibandingkan kontrol positif, yaitu orlistat (18,01 µg/mL). Dengan demikian, ekstrak daun mangga arum manis dan kweni dapat menghambat aktivitas lipase pankreas dan berpotensi untuk dikembangkan sebagai antiobesitas.AbstractObesity is caused by excess calories in the body. Reducing obesity can be done by inhibiting the absorption of fat as the main source of excess calories. One way that can be done to inhibit fat absorption is by inhibiting pancreatic lipase activity. This research was conducted to determine the lipase inhibitor activity of methanol extract of arumanis (Mangifera indica L. cv Arumanis) and kweni (Mangifera odorata Griff.) mango leaves. The research stages began with extraction using the maceration method, followed by testing the total phenol content, flavonoid content, and testing the lipase inhibitor activity of arumanis and kweni mango leaf extracts. The results of the total phenol content test showed that the total phenol in Arumanis and Kweni mango leaf extracts was 246.94 mg GAE/g and 176.11 mg GAE/g, respectively. The flavonoid content of Arumanis mango leaves is 129.95 mg.QE/g, while that of Kweni mango leaves is 26.50 mg.QE/g. Each extract was tested for its ability to inhibit pancreatic lipase in vitro. The IC50 values of Arumanis and Kweni mango leaf extracts are 61.55 µg/mL and 79.98 µg/mL. This value is higher than the positive control, namely orlistat (18.01 µg/mL). Thus, Arumanis and Kweni mango leaf extracts can inhibit pancreatic lipase activity and have the potential to be developed as an anti-obesity agent.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biometrik dan Kematangan Gonad Ikan Selar Kuning (Selaroides leptolepis Cuvier, 1833) Pada Perairan Muara Badak, Kalimantan Timur","authors":"Jusmaldi Jusmaldi, K. Dewi, Nova Hariani","doi":"10.15408/kauniyah.v16i2.1.27010","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.27010","url":null,"abstract":"AbstrakAspek biologis ikan Selar kuning Selaroides leptolepis (Cuvier, 1833) pada Perairan Muara Badak, Kalimantan Timur belum pernah diinformasikan. Penelitian ini bertujuan untuk menganalisis aspek biometrik dan kematangan gonad ikan Selar kuning. Pengumpulan sampel ikan dilakukan setiap bulan dari September hingga Desember 2021. Total 530 individu ikan dikoleksi menggunakan metode acak sederhana dari hasil tangkapan nelayan yang ikannya didaratkan di pelabuhan nelayan Toko Lima, Muara Badak. Panjang tubuh ikan diukur menggunakan kaliper digital dan bobot tubuh ditimbang menggunakan neraca digital. Hasil penelitian ini diperoleh panjang tubuh ikan berkisar 91,39–145,44 mm dan bobot 7,60–37,60 g. Modus dominan panjang tubuh ikan didapatkan pada rentang kelas 121,39–127,38 mm. Rasio kelamin adalah 1 jantan: 1,03 betina, yang menunjukkan rasio jenis kelamin seimbang. Hubungan panjang dan bobot tubuh memiliki koefisien korelasi yang kuat (r= 0,953), dengan persamaan regresi W= 0,0000003L3,281. Pola pertumbuhan allometrik positif dan nilai faktor kondisi relatif rata-rata 1,04 ± 0,060, yang mencerminkan kondisi pertumbuhan ikan relatif baik. Persentase tertinggi ikan matang gonad didapatkan pada bulan November dan Desember 2021, yang mengindikasikan ikan siap memijah. Kajian ini meginformasikan hubungan panjang dan bobot tubuh, pola pertumbuhan, faktor kondisi, rasio jenis kelamin, dan tingkat kematangan gonad ikan Selar kuning dari Perairan Muara Badak yang dapat digunakan untuk penilaian kesehatan populasi dan strategi pengelolaannya.AbstractBiological aspects of the Yellow-stripe scad Selaroides leptolepis (Cuvier, 1833) from Muara Badak Waters, East Kalimantan have never been informed anywhere. This research aimed to inform biometrics aspects and gonadal maturity of the Yellow-stripe scad. The collection of fish samples was carried out every month from September to December 2021. A total of 530 individual fish were collected using a simple random method from the catches of fishers who landed their fish at the Toko Lima fishing port, Muara Badak. Its body length was measured using a digital caliper and the body weight was weighed using a digital balance. The results of this research showed that the fish’s body length ranged from 91.39–145.44 mm and body weight ranged from 7.60–37.60 g. A modus of fish body length was found in the range class of 121.39–127.38 mm. The sex ratio is 1 male:1.03 female, which indicates a balanced sex proportion. The length and bodyweight relationship has a strong correlation coefficient (r= 0.953), with the regression equation was W= 0.0000003L3.281. The positive allometric growth and the relative condition factor value were an average of 1.04 ± 0.060, reflecting the fish’s relatively good growth conditions.The highest percentage of gonadal maturity of fishes was found in November and December 2021, which indicated that the fishes were ready to spawn. This research provides information on the relationship between leng","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hubungan Kekerabatan Padi Lokal di Kecamatan Teluk Batang Kabupaten Kayong Utara, Kalimantan Barat Berdasarkan Karakter Morfologi","authors":"Lipia Apriliani, Siti Ifadatin, E. R. Wardoyo","doi":"10.15408/kauniyah.v17i1.31057","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.31057","url":null,"abstract":"AbstrakOryza sativa memiliki banyak varietas yang merupakan sumber gen untuk mengendalikan sifat-sifat penting pada padi. Varietas padi lokal memiliki keunggulan seperti tahan terhadap  hawar daun bakteri, blas daun, dan kekeringan. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan varietas padi lokal di Kecamatan Teluk Batang Kabupaten Kayong Utara berdasarkan karakter morfologi. Penelitian dilakukan pada bulan April hingga September 2022 di lahan petani. Pengamatan terhadap 50 karakter morfologi batang, daun, malai, dan gabah/biji dilakukan selama pertumbuhan tanaman di lahan petani. Hasil penelitian diperoleh 12 varietas padi lokal yang semuanya merupakan padi Gogo/Ladang. Karakter vegetatif sebanyak 23 karakter yang terdiri dari 9 karakter bervariasi, 8 karakter tidak bervariasi, dan 6 karakter kuantitatif. Karakter generatif terdiri dari 27 karakter meliputi 14 karakter bervariasi, 5 karakter tidak bervariasi, dan 8 karakter kuantitatif. Berdasarkan analisis pengelompokan 12 varietas padi lokal memiliki tingkat kemiripan sebesar 60% dan terbagi menjadi 4 klaster pada tingkat kemiripan 64%. Klaster 1 terdiri dari varietas Padi Hitam (PH); klaster 2 yaitu Padi Merah (PM), Padi Serendah (PSE), Padi Lele (PL), Padi Rapi (PR), Raja Lele (RJ), dan Pulut Merah (PUM); klaster 3 yaitu padi Panda (PP), Umbang Wangi (UW), dan Umbang Ketupat (UK): klaster 4 yaitu Pulut Siam (PS) dan Pulut Minyak (PMI).Kata Kunci: Karakter morfologi; Kekerabatan; Padi lokal; Teluk BatangAbstractOryza sativa has many varieties which are sources of genes for controlling important traits in rice. Local rice varieties have advantages such as resistance to bacterial leaf blight, leaf blast and drought. This research aims to determine the relationship between local rice varieties in Teluk Batang District, North Kayong Regency based on morphological characters. The research was conducted from April to September 2022 on farmers' land. Observations of 50 morphological characters of stems, leaves, panicles and grain/seeds were carried out during plant growth on farmers' land. The research results obtained 12 local rice varieties, all of which are upland/field rice. There are 23 vegetative characters consisting of 9 varied characters, 8 unvaried characters and 6 quantitative characters. Generative characters consist of 27 characters including 14 varying characters, 5 non-varying characters, and 8 quantitative characters. Based on the grouping analysis, 12 local rice varieties have a similarity level of 60% and are divided into 4 clusters at a similarity level of 64%. Cluster 1 consists of Black rice varieties (PH), cluster 2 is Red rice (PM), Sedunia rice (PSE), Lele rice (PL), Rapi rice (PR), Raja lele (RJ), and Red pulut (PUM) , cluster 3 namely Panda rice (PP), Umbang wangi (UW), and Umbang ketupat (UK), cluster 4 namely Pulut siam (PS) and Pulut oli (PMI).","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVER AL-KAUNIYAH: JURNAL BIOLOGI VOL. 17 NO. 1 APRIL 2024","authors":"Cover Cover","doi":"10.15408/kauniyah.v17i1.36730","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.36730","url":null,"abstract":"","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keanekaragaman Makroinvertebrata di Sungai Kampai, Bengkulu","authors":"Abdul Rahman Singkam, Moh Aziz Pathori, Irwandi Ansori, Talib Chitheer","doi":"10.15408/kauniyah.v17i1.27892","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.27892","url":null,"abstract":"AbstrakPenelitian ini bertujuan untuk menganalisis keanekaragaman makroinvertebrata di Sungai Kampai, Bengkulu. Pengambilan sampel dilakukan di tiga stasiun, yaitu Muara Simpur, Pagar Agung, dan Lubuk Gio dengan dua kali periode sampling setiap stasiun, yaitu April 2019 mengikuti puncak musim hujan dan September 2019 mengikuti puncak musim kemarau. Pengambilan sampel makroinvertebrata menggunakan metode three minutes sampling. Hasil penelitian ditemukan 501 individu makroinvertebrata yang terdiri dari 25 spesies (24 genus; 22 famili; 5 kelas; 4 filum). Epeourus aculeatus merupakan spesies dengan kelimpahan relatif tertinggi secara keseluruhan, yaitu sebesar 62,08%. Indeks keanekaragaman (H’) berturut-turut adalah 1,72; 1,13; 1,80 mengindikasikan bahwa Sungai Kampai mengalami degradasi ringan. Jumlah individu Ephemeroptera, Plecoptera, dan Trichoptera (EPT)  per stasiun dari hulu ke hilir adalah 128; 196; dan 20 dengan Indeks Biotik Famili (IBF) adalah 3,34; 4; dan 4. Berdasarkan gabungan dari beberapa indeks ini menunjukkan bahwa kondisi Sungai Kampai masih tergolong sangat baik.AbstractThis research aims to analyze macroinvertebrate diversity in the Kampai River, Bengkulu. Sampling was carried out at three stations, namely Muara Simpur, Pagar Agung, and Lubuk Gio with two sampling periods for each station, namely April 2019 following the peak of the rainy season and September 2019 following the peak of the dry season. Macroinvertebrate sampling used the three minutes sampling method. The research results found 501 macroinvertebrate individuals consisting of 25 species (24 genera; 22 families; 5 classes; 4 phyla). Epeourus aculeatus is the species with the highest relative abundance overall, namely 62.08%. The diversity index (H') is 1.72; 1.13; 1.80 indicates that the Kampai River is experiencing mild degradation. The number of Ephemeroptera, Plecoptera and Trichoptera (EPT) individuals per station from upstream to downstream is 128; 196; and 20 with a Biotic Family Index (IBF) of 3.34; 4; and 4. Based on a combination of several indices, it shows that the condition of the Kampai River is still considered very good.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengembangan Primer Diagnostik Menggunakan Penanda mat-K Secara In Silico untuk Mendeteksi Kelangkaan Jenis Tumbuhan Di Indonesia","authors":"Hanina Dzikrina, Topik Hidayat, Siti Sriyati","doi":"10.15408/kauniyah.v17i1.27538","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.27538","url":null,"abstract":"Indonesia merupakan negara dengan kekayaan biodiversitas tertinggi di dunia. Terdapat sekitar 40.000 jenis tumbuhan yang tumbuh di Indonesia dan sebagian besar tumbuhan sudah menunjukkan kelangkaan. Penentuan kelangkaan suatu jenis tumbuhan dapat diketahui berdasarkan distribusi populasinya, namun membutuhkan waktu yang cukup lama. Ketidakstabilan genom akan terjadi pada jenis tumbuhan langka, karena tidak mampu beradaptasi pada ekosistem. Banyaknya tumbuhan yang terancam punah dan habitat asli yang rusak, maka mengharuskan para peneliti untuk mengetahui keberadaan dan melakukan pendataan terhadap keragaman jenis tumbuhan di Indonesia secara cepat. DNA barcoding merupakan teknik yang dikembangkan untuk mempercepat dan mempermudah proses identifikasi organisme dengan menggunakan potongan DNA tertentu. Tujuan dari penelitian ini adalah mencari primer spesifik untuk mendeteksi status kelangkaan pada tumbuhan menggunakan penanda mat-K secara in silico. Gen mat-K merupakan penanda umum yang direkomendasikan untuk analisis pada tumbuhan. Metode yang digunakan yaitu dengan pendekatan secara in silico karena waktu yang diperlukan relatif lebih singkat dan murah. Penelitian ini berhasil mendapatkan sepasang primer forward 1:F_1635–1657 dan primer reverse 1:R_2093–2113 dengan persentase keberhasilan amplifikasi yang dicapai sebesar 66%. Kedepannya, primer ini dapat digunakan untuk mengidentifikasi status kelangkaan pada tumbuhan.AbstractIndonesia is a country with the highest biodiversity wealth in the world. There are around 40,000 types of plants that grow in Indonesia and most of the plants are rare. Determining the rarity of a plant type can be determined based on its population distribution, but it takes quite a long time. Genome instability will occur in rare plant species, because they are unable to adapt to the ecosystem. The large number of plants that are threatened with extinction and their natural habitats are damaged requires researchers to quickly identify the existence and collect data on the diversity of plant species in Indonesia. DNA barcoding is a technique developed to speed up and simplify the process of identifying organisms using certain pieces of DNA. The aim of this research is to look for specific primers to detect rarity status in plants using mat-K markers in silico. The mat-K gene is a general marker recommended for analysis in plants. The method used is an in silico approach because the time required is relatively shorter and cheaper. This research succeeded in obtaining a pair of forward primers 1:F_1635–1657 and reverse primers 1:R_2093–2113 with a successful amplification percentage of 66%. In the future, this primer can be used to identify rarity status in plants.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Struktur Anatomis dan Uji Histokimia Kulit Buah Naga Merah (Hylocereus polyrhizus (Web.) Britton & Rose)","authors":"Lily Atiqah Husna, Laurentius Hartanto Nugroho","doi":"10.15408/kauniyah.v16i2.1.23602","DOIUrl":"https://doi.org/10.15408/kauniyah.v16i2.1.23602","url":null,"abstract":"AbstrakKulit buah naga memiliki potensi di berbagai bidang seperti obat-obatan, kosmetik, biopestisida, pewarna, dan perisa pada makanan. Hasil uji fitokimia sebelumnya membuktikan bahwa kulit buah naga merah mengandung senyawa metabolit sekunder. Namun, informasi mengenai struktur anatomis kulit buah naga dari spesies Hylocereus polyrhizus (Web.) Britton & Rose serta analisis histokimianya masih belum ada. Penelitian ini dilakukan bertujuan untuk mengobservasi struktur anatomis kulit buah naga merah dan mengidentifikasi keberadaan serta menganalisis distribusi senyawa fenolik, flavonoid, tanin, alkaloid, dan terpenoid dengan uji histokimia. Metode penelitian terdiri atas pembuatan preparat awetan dengan metode non-embedding dan observasi struktur anatomis kulit buah naga merah, uji histokimia terhadap senyawa fenolik, flavonoid, tanin, alkaloid, dan terpenoid, dan analisis distribusi persebarannya. Hasil penelitian menunjukkan bahwa struktur anatomis kulit buah naga merah terdiri atas bagian eksokarp (jaringan epidermis ganda dengan tipe permukaan cembung pada kulit secara umum dan berpapila pada epidermis atas sisik kulit buah) dan mesokarp (jaringan parenkim air kompak dan non kompak, sel lendir, kristal kalsium oksalat, sklereid, dan trakeid). Adapun senyawa metabolit sekunder yang ditemukan, yaitu fenolik yang terdistribusi pada trakeid, flavonoid pada struktur epidermis, dan alkaloid pada epidermis serta trakeid. Sebaliknya, senyawa tanin dan terpenoid tidak terdeteksi pada kulit buah naga merah.AbstractDragon fruit peel has potential in various fields such as medicine, cosmetics, biopesticides, dyes and food flavors. Previous phytochemical test results proved that red dragon fruit peel contains secondary metabolite compounds. However, information regarding the anatomical structure of dragon fruit peel from the species Hylocereus polyrhizus (Web.) Britton & Rose and its histochemical analysis is still missing. This research was carried out with the aim of observing the anatomical structure of red dragon fruit peel and identifying the presence and analyzing the distribution of phenolic compounds, flavonoids, tannins, alkaloids and terpenoids using histochemical tests. The research method consisted of making preserved preparations using the non-embedding method and observing the anatomical structure of red dragon fruit peel, histochemical tests on phenolic compounds, flavonoids, tannins, alkaloids and terpenoids, and analyzing their distribution. The results of the research show that the anatomical structure of red dragon fruit peel consists of the exocarp (double epidermal tissue with a convex surface type on the peel in general and papillae on the upper epidermis of the fruit peel scales) and the mesocarp (compact and non-compact water parenchyma tissue, mucus cells, crystals calcium oxalate, sclereids and tracheids). The secondary metabolite compounds found were phenolics distributed in tracheids, flavonoids in the structure of the epiderm","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara","authors":"Eka Nurdiani, Masriani Masriani, Rahmat Rasmawan, Rini Muharini, Rody Putra Sartika","doi":"10.15408/kauniyah.v17i1.31299","DOIUrl":"https://doi.org/10.15408/kauniyah.v17i1.31299","url":null,"abstract":"AbstrakKanker payudara didiagnosis menjadi penyebab utama kematian pada wanita di seluruh dunia. Beberapa penelitian terdahulu telah membuktikan bahwa tanaman simpur air (Dillenia suffruticosa (Griff.) Martelli) dapat menjadi agen antikanker yang potensial. Penelitian ini bertujuan untuk menentukan sitotoksisitas dan selektivitas dari fraksi kayu batang simpur air terhadap sel kanker payudara T47D, MCF-7, dan 4T1. Metode penelitian adalah eksperimental laboratorium dan uji aktivitas antikanker terhadap sel kanker payudara dilakukan secara in vitro menggunakan sel kanker payudara T47D, MCF-7, dan 4T1. Proses ekstraksi kayu batang simpur air menggunakan metode maserasi dengan pelarut metanol, selanjutnya difraksinasi dengan pelarut n-heksana dan etil asetat secara berturut-turut dan diuji aktivitas sitotoksiknya dengan metode MTT [3-(4,5-dimetiltiazol-2-il)-2,5 difeniltetrazolium bromida] assay. Hasil yang diperoleh, yaitu fraksi n-heksana menunjukkan sifat sitotoksik sedang pada sel kanker payudara T47D, MCF-7, dan 4T1 dengan nilai IC50 berturut-turut sebesar 65,27 ± 26,17; 66,98 ± 0,97; dan 69,23 ± 6,25 µl/mL dengan IS>2. Dengan demikian, fraksi n-heksana kayu batang simpur berpotensi dikembangkan sebagai antikanker baru.AbstractBreast cancer is diagnosed as the leading cause of death in women worldwide. Several previous studies have proven that the simpur air plant (Dillenia suffruticosa (Griff.) Martelli) can be a potential anticancer agent. This study aims to determine the cytotoxicity and selectivity of the simpur air stem wood fraction against T47D, MCF-7, and 4T1 breast cancer cells. This research is laboratory experimental and the anticancer activity test against breast cancer cells was carried out in vitro. The extraction process for Simpur Air wood stems uses the maceration method with methanol solvent, then fractionated with n-hexane and ethyl acetate solvents successively and tested for cytotoxic activity using the MTT method [3-(4,5-dimethylthiazol-2-yl)-2 .5 diphenyltetrazolium bromide] assay. The results obtained, namely the n-hexane fraction showed moderate cytotoxic properties on T47D, MCF-7, and 4T1 breast cancer cells with IC50 values of 65.27 ± 26.17 respectively; 66.98 ± 0.97; and 69.23 ± 6.25 µl/mL with IS>2. Thus, the n-hexane fraction of simpur stem wood has the potential to be developed as a new anticancer.","PeriodicalId":505278,"journal":{"name":"Al-Kauniyah: Jurnal Biologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139172753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}