Journal of Veterinary Medical Science最新文献

{"title":"Soy isoflavone yeast fermented extract inhibits contraction of rat ileal smooth muscle via opening of K⁺ channels and activation of intracellular cyclic adenosine monophosphate- and cyclic guanosine monophosphate-related pathways.","authors":"Shingo Yamazaki, Julia Kinuyama, Hisako Kaneda, Noriyasu Sasaki, Takeharu Kaneda","doi":"10.1292/jvms.25-0257","DOIUrl":"https://doi.org/10.1292/jvms.25-0257","url":null,"abstract":"<p><p>This study investigated the effects of soy isoflavone yeast fermented extract (soyF) and soy isoflavone yeast unfermented extract (soyN) on rat ileal smooth muscle contraction. SoyF and soyN inhibited carbachol (CCh)- or KCl-induced contraction in a concentration-dependent manner; however, these effects were stronger for CCh-induced contraction than that for KCl, and the relaxation effect was stronger for soyF than for soyN. SoyF-induced relaxation was attenuated by 4-aminopyridine (4-AP), a Kv channel inhibitor, and iberiotoxin (IbTX), a calcium-activated potassium channel (BK channel) inhibitor. This relaxation was also decreased by 9-(tetrahydrofuran-2-yl)-9h-purin-6-amine (SQ22536) and 1H-[1,2,4]oxadiazolo[4,3-a] quinoxalin-1-one (ODQ). Moreover, simultaneous treatment involving SQ22536 with 4-AP or IbTX further restored soyF-induced inhibition compared with each single treatment, whereas simultaneous treatment with ODQ and 4-AP or IbTX did not restore soyF-induced inhibition. SoyF increased cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in ileal strips. In β-escin-permeabilized muscle, CCh, guanosine 5'-O-(3-thiotriphosphate) (GTPγS), and phorbol-12,13-dibutyrate ( PDBu)-enhanced Ca²⁺-induced contraction, but soyF suppressed this Ca²⁺ sensitization. In summary, yeast-fermented treatment increased soy isoflavone-induced inhibition of ileal smooth muscle contraction. These data suggest that soyF-induced relaxation was due to increasing cAMP and cGMP related to activating adenylate cyclase and guanylate cyclase. Moreover, it was demonstrated that soyF-induced relaxation is due to cell membrane hyperpolarization and inhibition of contractile proteins. Cell membrane hyperpolarization is probably mediated by the activation of Kv and BK channels via cGMP-related signals. Furthermore, the inhibitory effect on the contractile protein is due to Ca²⁺ desensitization, probably through suppression of the Rho kinase/myosin phosphatase targeting subunit (MYPT) and/or the PKC/CPI-17 pathway.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful balloon angioplasty of right pulmonary coarctation with left pulmonary artery interruption in a cat: a long-term outcome follow-up case report.","authors":"Sunghoon Jeon, Gahyun Lee, Dahee Han, Jin-Kyung Kim, Namsoon Lee, Dongwoo Chang","doi":"10.1292/jvms.24-0501","DOIUrl":"10.1292/jvms.24-0501","url":null,"abstract":"<p><p>A 4-month-old, British Shorthair kitten was referred to our institute for evaluation of a heart murmur. Thoracic radiography and echocardiography revealed narrowing of the right pulmonary artery, accompanied by right atrial dilation and right ventricular hypertrophy. The systolic flow velocity across the stenotic lesion was 6.15 m/sec, with a pressure gradient of 151.42 mmHg. Computed tomography confirmed right pulmonary coarctation with concurrent interruption of the left pulmonary artery. Balloon angioplasty was performed, reducing the systolic flow velocity from 6.15 m/sec to 2.79 m/sec and relieving right ventricular pressure overload. One year later, thoracic radiography revealed left lung collapse and right lung hyperinflation; however, the patient remained asymptomatic. At 33 months post-diagnosis, the patient continued to thrive without any clinical signs.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1078-1085"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between farm-level antimicrobial usage and antimicrobial resistance in Escherichia coli isolates from healthy swine in ten farms in Japan.","authors":"Yukino Tamamura-Andoh, Sota Kobayashi, Itsuro Yamane, Mafumi Watanabe, Keerthi Siri Guruge, Ryuichi Uegaki, Nobuo Arai, Ayako Watanabe-Yanai, Taketoshi Iwata, Ken Katsuda, Masato Akiba, Masahiro Kusumoto","doi":"10.1292/jvms.25-0221","DOIUrl":"10.1292/jvms.25-0221","url":null,"abstract":"<p><p>We examined the antimicrobial resistance of 1,493 Escherichia coli isolates from 440 swine fecal samples (211 and 229 samples were obtained from the sow and fattening groups, respectively) on ten farms and analyzed the association between the antimicrobial resistance rate and the annual antimicrobial usage at the farm level adjusted by the daily defined dose in Japan. For most antimicrobial agents, the rate of resistance was greater on farms with higher levels of antimicrobial use, suggesting that the amount of the corresponding antimicrobial class used at the farm level may have affected the resistance rates. Some antimicrobial resistance was positively associated with the use of the corresponding or structurally related antimicrobial agents: resistance to tetracycline in the sow group and resistance to chloramphenicol, streptomycin, kanamycin, sulfonamide-trimethoprim, and nalidixic acid in the fattening group. In addition, for many antimicrobial-resistant strains, an association was found with the use of single or multiple antimicrobial classes other than the corresponding class: resistance to ampicillin and kanamycin in the sow group and resistance to ampicillin, cefazolin, cefoxitin, cefotaxime, tetracycline, chloramphenicol, streptomycin, kanamycin, gentamicin and nalidixic acid in the fattening group. In the case of the association with the noncorresponding class, most of the E. coli isolates were multidrug resistant, suggesting that there is a high possibility of coselection on farms and that reducing the use of multiple antimicrobial agents may be effective at decreasing antimicrobial resistance.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1053-1059"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcinosis circumscripta of the extremities and the tongue with multifocal arterial calcification secondary to chronic kidney disease in a cat.","authors":"Ai Koshikawa, Shingo Miki, Sangho Kim, Takashi Kimura, Keisuke Aoshima","doi":"10.1292/jvms.25-0042","DOIUrl":"10.1292/jvms.25-0042","url":null,"abstract":"<p><p>A 3-year-old neutered male mixed-breed cat was presented with right urinary tract obstruction caused by a urolith and severe atrophy of the left kidney. In the next year and a half, chronic kidney disease (CKD) progressed, and several masses were identified in the extremities, along with an ulcer on the tongue. Histologically, the extremity masses consisted of calcium deposits separated by fibrovascular septa, and the tongue ulcer showed severe neutrophilic infiltration with pervasive calcium deposition. These lesions were diagnosed as calcinosis circumscripta (CCs). Concurrently, multifocal mineralization of the aorta and of small- to medium-sized arteries in several organs indicated widespread vascular calcification. Both extremity and vascular lesions were likely secondary to the excessive calcium-phosphate product caused by CKD. Although CCs in either the extremities or tongue has been reported in cats, this case is notable for the presence of CCs in both extremities and tongue with systemic vascular calcification.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1095-1099"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction in finisher pig death rate on a farm in Japan following use of a commercial killed vaccine against porcine reproductive and respiratory syndrome virus.","authors":"Yoriko Yonezawa, Tomohiro Nakagami, Nobuyuki Tsutsumi, Nozomu Momma, Osamu Taira, Ryota Matsuyama, Kohei Makita","doi":"10.1292/jvms.24-0506","DOIUrl":"10.1292/jvms.24-0506","url":null,"abstract":"<p><p>PRRS (Porcine Reproductive and Respiratory Syndrome) is a costly disease in the global pig industry. In 2021, Nisseiken Co., Ltd. in Japan launched a PRRS-killed vaccine, PRRS-ME, but its effectiveness on reducing the death rate under field conditions remained unassessed. A longitudinal study on a PRRS-positive farm in Japan (July 2019-July 2022) evaluated its effect on death rates. Piglets on a PRRS-negative farrowing farm were vaccinated at 3 and 8 weeks of age in March 2021. The vaccinated piglets were introduced to the finisher farm, where a PRRS outbreak occurred. Vaccination coverage was not associated with the death rates of growers but was negatively associated with those of finishers. The predicted reduction in the death rate of finishers associated with complete vaccination coverage was 56.8%.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1060-1067"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144683453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection of animal coronaviruses into a bat cell line from the kidney of Pipistrellus abramus.","authors":"Keisuke Nakagawa, Shingo Miyawaki, Junji Moribe, Tatsunori Masatani, Taizo Saito, Yasuhiro Takashima","doi":"10.1292/jvms.24-0406","DOIUrl":"10.1292/jvms.24-0406","url":null,"abstract":"<p><p>Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1107-1111"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of anesthetic effects of medetomidine-midazolam-butorphanol and medetomidine-remimazolam-butorphanol administered subcutaneously and intraperitoneally in C57BL/6J mice.","authors":"Takeru Sasaki, Masaki Watanabe, Tomoki Omori, Ryosuke Nakanishi, Nobuya Sasaki","doi":"10.1292/jvms.25-0301","DOIUrl":"10.1292/jvms.25-0301","url":null,"abstract":"<p><p>Anesthesia management in experimental animals is essential for animal welfare and data reproducibility. In Japan, medetomidine-midazolam-butorphanol (MMB) is commonly used due to ketamine restrictions. We developed medetomidine-remimazolam-butorphanol (MRB) by replacing midazolam with remimazolam for faster metabolism and recovery. This study compared MMB and MRB administered intraperitoneally (i.p.) or subcutaneously (s.c.) in C57BL/6J mice of both sexes. Induction and recovery were assessed using immobilization, loss of righting reflex, and return of righting reflex. MRB i.p. produced the fastest induction and recovery with relatively low variability, suggesting potential utility for brief procedures. In contrast, s.c. administration resulted in consistent onset and recovery profiles for both agents, which may be advantageous for routine experimental use.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1092-1094"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144734970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of oocyst detection and PCR identification of chicken Eimeria species using intestinal contents of the rectum and cecum as different gastrointestinal tracts.","authors":"Aruto Takano, Chie Furuya, Daikichi Morinaga, April H Wardhana, Dyah H Sawitri, Toshimitsu Hatabu, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi","doi":"10.1292/jvms.25-0252","DOIUrl":"10.1292/jvms.25-0252","url":null,"abstract":"<p><p>In the present study, intestinal specimens were collected from layer chickens at a poultry slaughterhouse and used to evaluate infection with Eimeria species. Intestinal contents were extracted separately from the rectum (from the duodenum to rectum) and cecum and used for analyses. Using flotation detection methods, the number of eimerian oocysts per gram was higher in the cecum than rectum. Polymerase chain reaction analysis also detected a greater abundance of Eimeria species in the cecum than the rectum. These results suggest that analysis of cecal contents could be more useful for detecting and identifying Eimeria species.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1049-1052"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pre-contrast hyperattenuating metastatic lymph node on computed tomography due to iodide accumulation in a dog with thyroid carcinoma.","authors":"Sang-Kwon Lee, Su-Min Baek, Jin-Kyu Park, Jinsu Kang, Min Jang, Kija Lee","doi":"10.1292/jvms.25-0198","DOIUrl":"10.1292/jvms.25-0198","url":null,"abstract":"<p><p>An 11-year-old Bichon Frise presented with a right cervical mass. Computed tomography revealed a thyroid mass without significant lymphadenopathy; however, the caudal portion of the ipsilateral retropharyngeal lymph node exhibited marked hyperattenuation on pre-contrast images. Histopathology of this lymph node confirmed the presence of metastasis from thyroid adenocarcinoma. The caudal portion of the lymph node displayed dense tumor cells with abundant sodium/iodide symporter-positive cells. The observed hyperattenuation of the lymph node was presumed to result from iodide deposition associated with the metastasis of the thyroid tumor. This case suggests that even in the absence of lymph node enlargement, hyperattenuating lymph nodes should raise suspicion for metastasis in dogs with thyroid tumors. However, as this is a single case, further studies are needed to determine the broader applicability of this finding.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1100-1106"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144651083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of portal vein thrombosis with rivaroxaban in an American cocker spaniel with chronic hepatitis: a case report.","authors":"Ryo Niijima, Takehiko Kakizaki, Chun-Ho Park, Satoshi Kameshima, Yuya Otaka, Hiroaki Kawaguchi","doi":"10.1292/jvms.24-0517","DOIUrl":"10.1292/jvms.24-0517","url":null,"abstract":"<p><p>Anticoagulants are commonly used to treat venous thrombosis. Rivaroxaban, a direct factor Xa inhibitor, is widely used in veterinary medicine to prevent and treat thromboembolism. It has been used to manage portal vein thrombosis (PVT) in humans. As the incidence of PVT with chronic hepatitis (CH) is low in dogs, treatment methods have not been fully established. This case report describes the treatment of a 9-year-old male American cocker spaniel with CH and ascites, possibly due to concurrent PVT, using rivaroxaban. During treatment with rivaroxaban and diuretics, the patient showed improvement in portal hypertension, ascites, and edema. Herein, we report the first case of using rivaroxaban for the management of PVT with CH (treated with glucocorticoids) in a dog.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1086-1091"},"PeriodicalIF":1.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}