Journal of Veterinary Medical Science最新文献

Detection of haemosporidian parasite lineages from wild bird carcasses collected in eastern Japan. 日本东部野鸟尸体中血孢子虫寄生虫的检测。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-28 DOI: 10.1292/jvms.24-0267

Momoko Eki, Mizuki Sasaki, Yasukazu Muramatsu, Mari Kobayashi, Takanori Ishikawa, Leo Uchida

引用次数: 0

Evaluation of mesenchymal stem cells as feeder cells for the cultivation of corneal epithelial cell sheets in dogs. 间充质干细胞作为饲养细胞培养犬角膜上皮细胞片的研究。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-28 DOI: 10.1292/jvms.24-0532

Maresuke Morita, Naoki Fujita, Takayuki Nakagawa, Ryohei Nishimura, Keiko Tsuzuki

{"title":"Evaluation of mesenchymal stem cells as feeder cells for the cultivation of corneal epithelial cell sheets in dogs.","authors":"Maresuke Morita, Naoki Fujita, Takayuki Nakagawa, Ryohei Nishimura, Keiko Tsuzuki","doi":"10.1292/jvms.24-0532","DOIUrl":"https://doi.org/10.1292/jvms.24-0532","url":null,"abstract":"Corneal epithelial cell sheet transplantation is a promising therapy for reconstructing the ocular surface in canines with severe corneal disease. Cultivation of these sheets typically requires feeder cells that support the proliferation and maintenance of immature epithelial cells by secreting growth factors. However, using conventional murine 3T3 fibroblasts as feeder cells raises ethical and safety concerns. This study investigated canine adipose-derived mesenchymal stem cells (cADMSCs) and bone marrow mesenchymal stem cells (cBMMSCs) as alternative feeder cells. Both MSCs were cultured alone or with canine corneal epithelial cells. Changes in epidermal growth factor, keratinocyte growth factor, and hepatocyte growth factor (HGF) expression in both MSC types were evaluated by quantitative polymerase chain reaction. HGF expression was further assessed by western blot and enzyme-linked immunosorbent assay. Canine corneal epithelial cell sheets were cultivated with cADMSCs, cBMMSCs, or 3T3 fibroblasts, or without feeder cells, and their structure and quality were evaluated histopathologically. Results showed that cADMSCs and cBMMSCs originally expressed soluble factors, and HGF mRNA expression significantly upregulated when co-cultured with epithelial cells. However, no significant differences in sheet structure or quality were observed among feeder types. In conclusion, cADMSCs and cBMMSCs are viable alternatives to 3T3 fibroblasts for cultivating canine corneal epithelial cell sheets. Canine corneal epithelial cells interact with MSCs and promote the secretion of soluble factors from MSCs to promote epithelial cell growth. Moreover, canine corneal epithelial cells may maintain their proliferative capacity and immature state independent of feeder cells.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-element analysis of Traditional Mongolian Medicine Ga-Mu-Zhu-Er using inductively coupled plasma mass spectrometry. 电感耦合等离子体质谱法多元素分析蒙药嘎木珠尔。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-28 DOI: 10.1292/jvms.24-0414

Qin Na, Gu-Leng Amu, Li Mei, Zhi Gui, Lin Song

{"title":"Multi-element analysis of Traditional Mongolian Medicine Ga-Mu-Zhu-Er using inductively coupled plasma mass spectrometry.","authors":"Qin Na, Gu-Leng Amu, Li Mei, Zhi Gui, Lin Song","doi":"10.1292/jvms.24-0414","DOIUrl":"https://doi.org/10.1292/jvms.24-0414","url":null,"abstract":"Due to the high level of heavy metals and harmful elements, United States and Europe are precautious in importing herbal medicines, which has seriously hindered the internationalization process of traditional Chinese and Mongolian medicines. Ga-Mu-Zhu-Er (GMZE) contains a wide range of chemical compounds and inorganic elements. This paper describes a validation process for the simultaneous analysis of 30 elements in Traditional Mongolian Medicine GMZE samples with closed-vessel microwave digestion technique followed by inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The samples digested with concentrated nitric acid (65%, v/v) revealed recoveries ranging from 91.9 to 107.7%. Several other parameters, including linearity, specificity, the limit of detection (LOD) and quantification (LOQ), precision of measurement, repeatability, intermediate precision and accuracy, were validated. The LOD were found to be in the range of 0.00015-0.21 µg/L, and the LOQ were between 0.00046 to 0.64 µg/L. Three GMZE samples were analyzed. Among the analyzed elements, Ca, As, and Hg were the most abundant, whereas, Be and Ag were present in low concentrations in all the samples. The results obtained in our study demonstrated that the developed ICP-MS method is simple, fast, and reliable, which could be used for the routine quantitation of these 30 essential and non-essential trace elements in GMZE as well as other Traditional Chinese Medicines and dietary supplements, with acceptable analytical performance.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Successful treatment of patent ductus arteriosus and severe pulmonary hypertension using sildenafil and beraprost sodium in two dogs. 西地那非联合伯拉前列钠成功治疗2只犬动脉导管未闭和重度肺动脉高压。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-26 DOI: 10.1292/jvms.25-0097

Keisuke Sugimoto, Yohei Mochizuki, Takamasa Itoi, Kanako Tsuji, Asuka Iwano, Yuki Shimizu, Yuko Wada, Teppei Kanda

引用次数: 0

Effect of birth month on endocrine function in Thoroughbred foals born in Hokkaido, the northern part of Japan. 出生月份对日本北海道纯种马驹内分泌功能的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-23 DOI: 10.1292/jvms.25-0061

Mutsuki Ishimaru, Koichi Kume, Harutaka Murase, Fumio Sato, Akira Matsui, Hajime Ohmura, Kazuyoshi Taya

{"title":"Effect of birth month on endocrine function in Thoroughbred foals born in Hokkaido, the northern part of Japan.","authors":"Mutsuki Ishimaru, Koichi Kume, Harutaka Murase, Fumio Sato, Akira Matsui, Hajime Ohmura, Kazuyoshi Taya","doi":"10.1292/jvms.25-0061","DOIUrl":"https://doi.org/10.1292/jvms.25-0061","url":null,"abstract":"Thoroughbreds born early in the year are believed to have an advantage in racing. However, the development of endocrine functions in early-born foals in the cold winter climate is unclear. The present study was conducted to elucidate the effect of birth month on the development of endocrine function in Thoroughbred foals born from February to May in Hokkaido by comparing hypothalamic-pituitary axis, adrenal and thyroid functions from birth to 24 weeks of age. Eighty-seven Thoroughbred foals were used to compare the changes in circulating luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, cortisol, and thyroxine among four birth month groups for each sex. The present study revealed that as foals grow, the hypothalamic-pituitary axis develops, allowing them to secrete LH and FSH in response to prolonged day length from about 10 weeks of age, regardless of birth month for both sexes. In addition, the increase in prolactin concentrations from May to August suggests that postnatal foals may have the ability to respond to prolonged daylength immediately after birth, independent of growth. Furthermore, circulating cortisol and thyroxine in the foals at birth were elevated regardless of birth month, suggesting that adrenal and thyroid functions were fully activated for the rapid growth during late gestation and adaptation to the external environment that occurs early postnatal period. In early-born foals, these hormone concentrations were found to be within the normal range, indicating that development of foals were not affected by the harsh Hokkaido winter.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No evidence of bovine leukemia virus proviral DNA in a widely used commercially frozen semen in Japan. 在日本广泛使用的商业冷冻精液中没有牛白血病病毒前病毒DNA的证据。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-22 DOI: 10.1292/jvms.25-0212

Aronggaowa Bao, Sonoko Watanuki, Ryosuke Matsuura, Yasunobu Matsumoto, Hiroyuki Shimizu, Ryusaku Kawata, Yoko Aida

{"title":"No evidence of bovine leukemia virus proviral DNA in a widely used commercially frozen semen in Japan.","authors":"Aronggaowa Bao, Sonoko Watanuki, Ryosuke Matsuura, Yasunobu Matsumoto, Hiroyuki Shimizu, Ryusaku Kawata, Yoko Aida","doi":"10.1292/jvms.25-0212","DOIUrl":"https://doi.org/10.1292/jvms.25-0212","url":null,"abstract":"Bovine leukemia virus (BLV) is a global concern that significantly affects the productivity of dairy and beef cattle, leading to considerable economic losses. BLV is primarily transmitted through infected lymphocytes via both horizontal and vertical routes. Vertical transmission occurs through the colostrum, uterus, and placenta. Although the possibility of BLV transmission through infected semen during artificial insemination is a recognized concern. No such cases have been reported in Japan, while studies from other countries reported a low risk. In this study, we aimed to evaluate the presence of BLV provirus in commercially frozen semen to assess the potential risk of infection in Japan. A sufficient quantity of genomic DNA with adequate purity was extracted from 191 widely used commercially frozen semen samples obtained from top-ranking bulls in the annual market survey in Japan between 2000 and 2022 using the phenol-chloroform extraction method. No BLV provirus was detected in any of the 191 frozen semen samples using either BLV-CoCoMo-qPCR-2 targeting the BLV long-terminal repeat region or nested polymerase chain reaction targeting BLV gp51 env gene. Similarly, the results are consistent with reports from other countries. Therefore, the findings of this study suggest a low risk of BLV transmission through frozen semen in Japan. Our study further supports that artificial insemination combined with the selection of non-infected cows for breeding are effective measures to prevent the spread of infection.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study on reproduction in alpacas (Lama pacos) and cria growth after birth in Japan: the relationship between parturition and climate conditions. 日本羊驼（Lama pacos）出生后繁殖和cria生长的回顾性研究：分娩与气候条件的关系

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-22 DOI: 10.1292/jvms.25-0200

Misa Arafurue, Chihiro Kanno, Shogo Sato, Yosuke Maeda, Fumiaki Takahashi, Kenji Momozawa, Masashi Nagano

引用次数: 0

A newly developed temperature-sensitive Mycoplasma synoviae live attenuated strain prevents pathological lesions of the respiratory and reproductive tracts in chickens caused by a wild-type M. synoviae strain. 一种新开发的温度敏感型滑膜支原体减毒活株可预防由野生型滑膜支原体引起的鸡呼吸道和生殖道病理病变。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-21 DOI: 10.1292/jvms.25-0021

Chia-Chi Liu, Chihiro Suzuki, Katsuo Sato, Hiroki Otomo, Yoshihiro Shimoji, Eiji Oishi

{"title":"A newly developed temperature-sensitive Mycoplasma synoviae live attenuated strain prevents pathological lesions of the respiratory and reproductive tracts in chickens caused by a wild-type M. synoviae strain.","authors":"Chia-Chi Liu, Chihiro Suzuki, Katsuo Sato, Hiroki Otomo, Yoshihiro Shimoji, Eiji Oishi","doi":"10.1292/jvms.25-0021","DOIUrl":"https://doi.org/10.1292/jvms.25-0021","url":null,"abstract":"A field isolate of Mycoplasma synoviae, designated D14-383, caused pathogenic lesions in the air sac, trachea, lung, and ovary and induced a decline or stop in egg production and eggshell apex abnormalities in chickens after intratracheal inoculation. A live M. synoviae vaccine candidate was developed after in vitro passaging at 32°C from a wild-type M. synoviae strain originally isolated from the trachea of a 5-month-old asymptomatic layer chicken. In this study, the vaccine efficacy of the attenuated vaccine candidate strain against M. synoviae infection caused by the D14-383 strain was investigated. Eye-drop vaccination of 4-week-old chickens with 105.9 colony-forming units (CFU)/dose of the attenuated strain induced high levels of anti-M. synoviae antibodies, which were tested by serum plate agglutination, and a 95% seroconversion rate was maintained in the vaccinated birds for 108 weeks after vaccination. In the vaccinated birds, the air sac lesion score was 0.93 versus 4.0 in the unvaccinated group, as revealed by postmortem examination 7 days after challenge infection with the D14-383 strain. Moreover, the percentage of ovarian lesions was 15.9% (7/44 birds) and 73.3% (11/15 birds) in the vaccinated and unvaccinated groups, respectively. During the observation period after vaccination, egg production stopped in the unvaccinated birds but not in the vaccinated birds. Thus, the attenuated strain was proven to induce 2 years of protective immunity against M. synoviae infection in chickens.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life table analysis of feline polycystic kidney disease in Japan. 日本猫多囊肾病的生命表分析。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-20 DOI: 10.1292/jvms.25-0080

Takeru Enomoto, Saori Kobayashi, Naomi Ishiguro

{"title":"Life table analysis of feline polycystic kidney disease in Japan.","authors":"Takeru Enomoto, Saori Kobayashi, Naomi Ishiguro","doi":"10.1292/jvms.25-0080","DOIUrl":"https://doi.org/10.1292/jvms.25-0080","url":null,"abstract":"Feline polycystic kidney disease (PKD) is an inherited renal disorder observed in various breeds. Analyses of life expectancy and distribution of age at death among cats are not well documented. The current study preliminarily assessed life expectancy, cumulative survival rates, and distribution of age at death in cats with PKD by performing a follow-up investigation. This retrospective cohort study was conducted on 300 cats that tested positive for the feline PKD1 variant (c.10063C > A) in Japan. A life table analysis was performed, and a distribution graph of age at death was generated. The life expectancy at birth was approximately 12.7 years, with a 5- and 10-year cumulative survival rates of 95.1% and 61.3%, respectively. Of the 300 cats, 48 (16.0%) progressed to uremia, and 62 (20.7%) died of renal failure and other causes. The median age at death in 62 cats was 8 years, with the first decile being 5 years. The highest number of deaths was at the age of 7 years. The cumulative survival rate began to decline significantly at the age of 6-7 years. This study first performed a survival analysis of feline PKD and provided an important basis for understanding the patterns of overall mortality associated with this genetic disorder. Our findings emphasized the importance of frequent examination at a young age, with consideration of the remarkable decrease in the cumulative survival rate between the ages of 6 and 7 years.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effect of paeoniflorin on the LuxS/AI-2 quorum sensing system and the virulence of Glaesserella parasuis. 芍药苷对副猪绿脓杆菌LuxS/AI-2群体感应系统及毒力的抑制作用

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-05-15 Epub Date: 2025-03-31 DOI: 10.1292/jvms.25-0008

Haojie Zhang, Qiwei Song, Xu Wang, Xiaoxue Yue, Zhikui Wang, Cong Wang, Xiulei Cai

{"title":"Inhibitory effect of paeoniflorin on the LuxS/AI-2 quorum sensing system and the virulence of Glaesserella parasuis.","authors":"Haojie Zhang, Qiwei Song, Xu Wang, Xiaoxue Yue, Zhikui Wang, Cong Wang, Xiulei Cai","doi":"10.1292/jvms.25-0008","DOIUrl":"10.1292/jvms.25-0008","url":null,"abstract":"Glaesserella parasuis, the causative agent responsible for Glässer's disease in swine, leads to severe polyserositis, meningitis, and arthritis, posing a significant threat to swine production. The LuxS/AI-2 quorum sensing system is crucial for G. parasuis growth, biofilm formation, and pathogenicity. Quorum sensing inhibitors, such as paeoniflorin (PF), offer a potential strategy to mitigate antibiotic resistance. This study evaluated PF's effects, alone and combined with antibiotics, on quorum sensing, biofilm formation, and virulence gene expression of drug-resistant G. parasuis. PF significantly reduced AI-2 production, biofilm formation, and virulence genes expression in vitro. A viable model for G. parasuis infection was established using KM mice, and demonstrated that a low-dose combination of PF and lincomycin effectively mitigated organ damage, reduced bacterial load in the liver, and improved survival rates compared to individual treatments in vivo. These findings suggest PF as a promising quorum sensing and virulence inhibitor for controlling G. parasuis infections. Further research is warranted to optimize its clinical application.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"565-574"},"PeriodicalIF":1.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0