Journal of Veterinary Medical Science最新文献

Long-term management of paroxysmal atrioventricular block with Wenckebach cycles in a dog. 用 Wenckebach 循环治疗犬阵发性房室传导阻滞的长期疗效。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-26 DOI: 10.1292/jvms.24-0521

Shohei Morita, Shiho Taguchi, Takahiro Kondo, Aritada Yoshimura, Shiori Ikoma, Takahiro Ohmori, Daiki Hirao, Noboru Machida, Hisashi Hirose, Ryuji Fukushima

引用次数: 0

Negative impact of porcine circovirus type 2 infection on the efficacy of classical swine fever vaccine in a field farm. 猪圆环病毒 2 型感染对田间养殖场经典猪瘟疫苗疗效的负面影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-24 DOI: 10.1292/jvms.24-0496

Keisuke Kuwata, Keko Otsu, Shuko Inoha, Yoko Kimura, Hiroshi Aoki, Yoshihiro Sakoda

{"title":"Negative impact of porcine circovirus type 2 infection on the efficacy of classical swine fever vaccine in a field farm.","authors":"Keisuke Kuwata, Keko Otsu, Shuko Inoha, Yoko Kimura, Hiroshi Aoki, Yoshihiro Sakoda","doi":"10.1292/jvms.24-0496","DOIUrl":"https://doi.org/10.1292/jvms.24-0496","url":null,"abstract":"Porcine circovirus type 2 (PCV2) induces wasting and immunosuppression in pigs and is widely transmitted in pig farms worldwide. Classical swine fever (CSF) is a particularly important contagious disease in pigs. In CSF-endemic areas, such as Japan, thorough vaccination is performed, and effective use of the CSF vaccine is important to prevent outbreaks. This study investigated the impact of PCV2 infection on the immune response to CSF vaccines in field farms. The mortality rate of fattening pigs on the farm was investigated, and pig sera were used to measure the PCV2 viral load and neutralizing antibody titer of CSF as indicators of CSF vaccine efficacy. Results indicated a sharp increase in mortality rate, PCV2 detection rate reaching 100%, and high viral load, whereas CSF antibody titers were significantly lower in the fattening pig herd. After PCV2 inactivated vaccination was initiated, the mortality rate, PCV2 detection rate, and viral load in fattening pigs decreased, and CSF antibody titers also improved. Furthermore, there was a correlation between higher PCV2 viral load and lower CSF antibody titers in this farm. In contrast, other PCV2-vaccinated farms had higher CSF antibody levels. These results indicate that PCV2 infection negatively affects the efficacy of CSF vaccines, and the control of PCV2 in field farms is important.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First documented case of Streptococcus uberis infection in a whooper swan.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-24 DOI: 10.1292/jvms.24-0266

Goun Park, JaeKyeom Kim, SoHyeon Kim, Su-Jin Park, SeunMin Oh, Dahyun Hwang, Chunghyun Kim

引用次数: 0

Effects of trastuzumab emtansine on canine osteosarcoma cells.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-21 DOI: 10.1292/jvms.24-0201

Kosei Sakai, Daiki Kato, Junka Yoshinaka, Muneki Honnami, Yosuke Takahashi, Namiko Ikeda, Susumu Aoki, Takaaki Iguchi, Qin Shiyu, Miki Ando, Satoshi Kameshima, Shunsuke Shimamura, Takayuki Nakagawa

{"title":"Effects of trastuzumab emtansine on canine osteosarcoma cells.","authors":"Kosei Sakai, Daiki Kato, Junka Yoshinaka, Muneki Honnami, Yosuke Takahashi, Namiko Ikeda, Susumu Aoki, Takaaki Iguchi, Qin Shiyu, Miki Ando, Satoshi Kameshima, Shunsuke Shimamura, Takayuki Nakagawa","doi":"10.1292/jvms.24-0201","DOIUrl":"https://doi.org/10.1292/jvms.24-0201","url":null,"abstract":"Human epidermal growth factor receptor 2 (HER2) is a known therapeutic target in canine osteosarcoma (OSA); however, the efficacy of anti-HER2 antibody drugs remains unclear. This study aimed to investigate the effects of the anti-HER2 antibody drugs including trastuzumab and trastuzumab emtansine (T-DM1) on canine OSA cell lines in vitro and in vivo. Four canine OSA cell lines (HMPOS, POS, OOS, and HOS) were used. Western blotting revealed HER2 protein expression in all the cell lines. Although water-soluble tetrazolium salt assays showed growth inhibitory activity of trastuzumab and T-DM1 against all the cell lines in vitro, the activity of T-DM1 was significantly stronger than that of trastuzumab. Flow cytometric analysis of the canine OSA cell line (HMPOS) revealed that T-DM1, but not trastuzumab, significantly increased the sub-G1 phase fraction in cell cycle analyses and the percentage of early and late apoptotic cells in annexin V apoptotic assays. For in vivo experiments, canine OSA cells (HMPOS) were subcutaneously injected into nude mice. Six days after inoculation, trastuzumab, T-DM1, or the vehicle control was administered intraperitoneally once per week. Survival until the tumor volume in the canine OSA-engrafted mice reached mean final tumor volume in the T-DM1 group was significantly longer in the T-DM1 group, but not in the trastuzumab group, compared to the vehicle control group. These findings indicated that T-DM1 exerts antitumor effects on canine OSA cells in vitro and in vivo, possibly by inducing apoptosis due to DM1.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective efficacy of the NcGRA7-deficient parasite as a live attenuated vaccine against Neospora caninum infection in mice.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-20 DOI: 10.1292/jvms.24-0460

Ahmed M Abdou, Yoshifumi Nishikawa

{"title":"Protective efficacy of the NcGRA7-deficient parasite as a live attenuated vaccine against Neospora caninum infection in mice.","authors":"Ahmed M Abdou, Yoshifumi Nishikawa","doi":"10.1292/jvms.24-0460","DOIUrl":"https://doi.org/10.1292/jvms.24-0460","url":null,"abstract":"Live vaccination is the most protective method against bovine neosporosis, which is the major cause of bovine abortion globally. Here, the Neospora caninum parenteral strain Nc1 and NcGRA7-deficient N. caninum (NcGRA7KO), which is less virulent in mice, were evaluated as potential live vaccines. BALB/c mice were subcutaneously inoculated with high (1 × 105) or low (1 × 104) doses of tachyzoites. At high doses, Nc1-inoculated female mice presented decreased body weight gain and increased clinical signs and died before challenge infection with green fluorescent protein (GFP)-expressing Nc1 (Nc1-GFP), whereas NcGRA7KO-inoculated animals exhibited increased survival before and after challenge infection. Although inoculation of female mice with Nc1 or NcGRA7KO resulted in a lower brain parasite number of challenged Nc1-GFP than in noninoculated animals, the total brain parasite burden in NcGRA7KO-infected mice decreased compared with that in Nc1-infetced animals. At low dose of NcGRA7KO, increased survival rates of mice and lower total brain parasite number were observed compared with high dose of NcGRA7KO. In male mice, a significant lower brain parasite burden of Nc1-GFP was observed in both high and low doses of NcGRA7-inoculated mice, and the total parasite number in the brains of low dose of NcGRA7KO-inoculated animals was lower than that in the brains of high dose of NcGRA7KO-inoculated or noninoculated animals. In conclusion, these results suggest that NcGRA7KO parasites have potential for use as a live vaccine against N. caninum infection.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143702051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study of the correlation of intraocular pressure and ultrasound biomicroscopy parameters with cataract maturity in small breed dogs.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-20 DOI: 10.1292/jvms.24-0428

Yeajin Jang, Seonmi Kang, Kangmoon Seo

{"title":"A retrospective study of the correlation of intraocular pressure and ultrasound biomicroscopy parameters with cataract maturity in small breed dogs.","authors":"Yeajin Jang, Seonmi Kang, Kangmoon Seo","doi":"10.1292/jvms.24-0428","DOIUrl":"https://doi.org/10.1292/jvms.24-0428","url":null,"abstract":"There is a clinical challenge in decision-making regarding prophylactic glaucoma treatment based solely on intraocular pressure (IOP) trends for long-term cataract management or before phacoemulsification surgery in canines. This study aimed to compare IOP and ultrasound biomicroscopy (UBM) parameters with cataract maturity in small dog breeds. We conducted a retrospective review of medical records pertaining to ophthalmic examinations and UBM assessments of small breed dogs diagnosed with cataracts at the Veterinary Medical Teaching Hospital of Seoul National University from January 2020 to August 2024. UBM images obtained under topical anesthesia were analyzed to quantify various parameters. Subsequently, the 47 eyes examined were classified into four groups according to cataract maturity. The ciliary cleft area (CCA), ciliary cleft length (CCL) and width (CCW), and iridocorneal angle (ICA) were measured. The measured values were compared to determine their degree of correlation with cataract maturity. Statistical analyses of patient demographics were also performed. Both CCA (P=0.0024) and CCL (P=0.0004) decreased significantly with increasing cataract maturity. However, IOP, CCW, and ICA were not significantly associated with cataract maturity. Age and sex did not have significant effects on any dependent variable. Among the UBM parameters, CCA and CCL demonstrated a significant correlation with cataract maturity, indicating structural alterations in the ICA might develop in association with cataracts, independent of direct IOP effects. These findings underscore the importance of considering prophylactic glaucoma management in patients with advanced cataracts to mitigate potential complications.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative pathology of ducks experimentally inoculated with three different recombinant Newcastle disease viruses. 实验性接种三种不同重组新城疫病毒的鸭的病理学比较。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-19 DOI: 10.1292/jvms.25-0002

Sohta Hishikawa, Yuji Sunden, Aoi Imamura, Junta Nishibayashi, Chiharu Hidaka, Hiroshi Ito, Toshihiro Ito, Takehito Morita

{"title":"Comparative pathology of ducks experimentally inoculated with three different recombinant Newcastle disease viruses.","authors":"Sohta Hishikawa, Yuji Sunden, Aoi Imamura, Junta Nishibayashi, Chiharu Hidaka, Hiroshi Ito, Toshihiro Ito, Takehito Morita","doi":"10.1292/jvms.25-0002","DOIUrl":"https://doi.org/10.1292/jvms.25-0002","url":null,"abstract":"Although velogenic Newcastle disease viruses (NDVs) generally show low pathogenicity in waterfowl such as ducks, lethal infection by NDVs has been reported sporadically. A previous study revealed that a serially-passaged NDV isolate showed increased virulence in ducks, and that the viral proteins harbored a total of 11 amino acid substitutions. Among them, the fusion protein (F), matrix protein (M), and hemagglutinin-neuraminidase (HN) were suspected to be involved in enhanced virulence. The present study aimed to clarify the association between genetic mutations in NDVs and pathogenicity in ducks by comparing histopathological findings in ducks inoculated with three recombinant (r)NDVs: rM44 (Q44R substitution in M), rF142 (I142M substitution in F), and rM+F+HN (Q44R, N123D, and D342N substitutions in M; I142M and E304K substitutions in F; and G538R substitution in HN; three viral proteins compatible with amino acid sequences from passaged NDV). Seven-day-old ducks were intranasally inoculated with rM44, rF142, or rM+F+HN. Gross and histopathological lesions in the heart, thymus, brain, and pancreas were more severe in ducks inoculated with rM+F+HN than in ducks inoculated with rM44 or rF142. In particular, myocarditis associated with NDV antigens and mixed inflammatory cells was prominent in rM+F+HN-inoculated ducks. Therefore, the increased virulence of NDV in ducks is not due to a single amino acid substitution in one protein, but rather to the synergistic effects of multiple mutations in three viral proteins.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of changes in the habitat area of Oncomelania hupensis nosophora, the intermediate snail host of Schistosoma japonicum, in the Obitsu River basin in Chiba Prefecture, Japan, using geographic information systems.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-17 DOI: 10.1292/jvms.24-0515

Naoko Nihei, Kensuke Taira, Mayuko Yonejima, Osamu Komagata, Hiroyuki Suzuki, Kan-Ichiro Mochizuki, Tomoki Nakaya

{"title":"Analysis of changes in the habitat area of Oncomelania hupensis nosophora, the intermediate snail host of Schistosoma japonicum, in the Obitsu River basin in Chiba Prefecture, Japan, using geographic information systems.","authors":"Naoko Nihei, Kensuke Taira, Mayuko Yonejima, Osamu Komagata, Hiroyuki Suzuki, Kan-Ichiro Mochizuki, Tomoki Nakaya","doi":"10.1292/jvms.24-0515","DOIUrl":"https://doi.org/10.1292/jvms.24-0515","url":null,"abstract":"Human schistosomiasis is a serious parasitic disease caused by infection with Schistosoma japonicum. In order to establish monitoring sites in the S. japonicum endemic area in the Obitsu River basin in Chiba Prefecture, Japan, we investigated the habitat of the intermediate snail host, Oncomelania hupensis nosophora using geographic information systems (GIS). A series of thematic maps of soil types, land-use, and past wetlands were compared to the distributions of patients and O. h. nosophora habitats to identify environmental conditions associated with high risk of the disease. In addition, we divided the period from the 1600s to the present into four sub-periods to estimate how the habitat of O. h. nosophora has changed. Our study identified the present risk areas in the Obitsu River basin that should be monitored consecutively, taking into account future global environmental changes that may have the potential to promote re-emergence of the disease.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of teat canal score to milk somatic cell count, pathogen infection, and antimicrobial components in Holstein dairy cows.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-12 DOI: 10.1292/jvms.24-0510

Keiichi Hisaeda, Naomi Ohta, Naoki Yamamoto, Akira Goto, Yoichi Inoue, Yasunori Shinozuka, Naoki Suzuki, Naoki Isobe

{"title":"Relationship of teat canal score to milk somatic cell count, pathogen infection, and antimicrobial components in Holstein dairy cows.","authors":"Keiichi Hisaeda, Naomi Ohta, Naoki Yamamoto, Akira Goto, Yoichi Inoue, Yasunori Shinozuka, Naoki Suzuki, Naoki Isobe","doi":"10.1292/jvms.24-0510","DOIUrl":"https://doi.org/10.1292/jvms.24-0510","url":null,"abstract":"This study examined the relationship between teat canal score (TCS), somatic cell count (SCC), pathogen infection, and milk antimicrobial components in each quarter of milking cows. A total of 110 quarters of 28 healthy Holstein milking cows were used for the TCS and collection of milk samples. Milk samples were analyzed for SCC, antimicrobial components (S100A7 and lingual antimicrobial peptide (LAP)), and acute phase protein (serum amyloid A (SAA)). Milk samples with SCC of at least 105 cells/mL were cultured to isolate and identify the causative organism. A total of 110 teats were classified, TCS of 1, 2, 3, 4 in 60.9% (67), 26.4% (29),11.8% (13) and 0.9% (1) teats, respectively. A positive correlation (r=0.6915, P<0.0001) was found between the TCS and SCC in all teats. Pathogens were detected in 100% of milk samples from TCS ≥3 milk and 91% of those from TCS 2. However, pathogens were detected in only 33% of milk samples from TCS 1, a significantly (P<0.0001) lower detection rate than those from TCS 2 and ≥3. The LAP concentration in the group with detected pathogen was significantly (P<0.01) higher than that in the no growth group. The results suggest that in quarters with higher TCS, SCC and LAP concentrations were significantly higher, milking it easier to detect pathogens due to the greater likelihood of bacterial invasion.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oligosarcoma with chondroid metaplasia in a French bulldog.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-11 DOI: 10.1292/jvms.25-0046

Kio Yoshida, James K Chambers, Masashi Terao, Hiroaki Kamishina, Daisuke Hasegawa, Kazuyuki Uchida

引用次数: 0