{"title":"Antemortem diagnosis combining computed tomography and air sac fluid aspiration for aspergillosis: a case report in a gentoo penguin (Pygoscelis papua).","authors":"Naoya Matsumoto, Megumi Itoh, Takahito Toyotome, Kenichi Watanabe, Manabu Yamada, Kyogo Hagino, Sakurako Neo, Kazutaka Yamada","doi":"10.1292/jvms.25-0377","DOIUrl":"https://doi.org/10.1292/jvms.25-0377","url":null,"abstract":"<p><p>Pulmonary aspergillosis is a fatal disease with high mortality in captive penguins in zoos and aquariums; however, antemortem identification of Aspergillus spp. remains challenging. A gentoo penguin (Pygoscelis papua) exhibiting respiratory clinical signs showed fluid accumulation in the interclavicular air sac on computed tomography (CT). The fluid was aspirated via puncture using a sterile needle in the prone position, as during CT. The fluid sample was positive for A. fumigatus in fungal culture. After the penguin died, infection of A. fumigatus was confirmed in the air sac fluid collected postmortem pathological examination. These findings suggest that combining computed tomography and air sac aspiration is a promising approach for the antemortem diagnosis of aspergillosis in penguins.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A case of oligodendroglioma in a sea otter (Enhydra lutris).","authors":"Chiho Kezuka, Hisaki Tokuno, Mizuki Kuramochi, Momoyo Muramoto, Keisuke Furuta, Hideo Akiyoshi, Miyuu Tanaka, Takeshi Izawa, Mitsuru Kuwamura","doi":"10.1292/jvms.26-0140","DOIUrl":"https://doi.org/10.1292/jvms.26-0140","url":null,"abstract":"<p><p>A 19-year-old male sea otter (Enhydra lutris) exhibited systemic seizures, declining consciousness, temporary cardiopulmonary arrest, and right-side rotation. The sea otter died approximately one month after its initial seizure. Postmortem magnetic resonance imaging examination revealed a high-intensity region in the left frontal lobe. At necropsy, a gelatinous lesion in the left frontal lobe of the brain was revealed. Histopathologically, the lesion consisted of neoplastic proliferation of round to polyhedral monomorphic cells with a honeycomb growth pattern, small round nuclei, and cytoplasmic halos. The neoplastic cells were immunopositive for oligodendrocyte transcriptional factor-2 (OLIG2) and immunonegative for glial fibrillary acidic protein (GFAP). In this study, we describe the clinical signs and histopathological features of oligodendroglioma, which have rarely been reported in sea otters.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fusion of the musculocutaneous nerve with the median nerve in a Japanese raccoon dog (Nyctereutes procyonoides viverrinus).","authors":"Nonoha Miyai, Motoharu Oishi, Tetsuhito Kigata","doi":"10.1292/jvms.26-0167","DOIUrl":"https://doi.org/10.1292/jvms.26-0167","url":null,"abstract":"<p><p>Various patterns of the relationship between the musculocutaneous and median nerves have been reported. This is the first report in Canidae, a Japanese raccoon dog, of the musculocutaneous nerve fused into the median nerve in the brachial plexus. The musculocutaneous nerve gave rise to the only branch to the coracobrachialis muscle and fused into the median nerve. The median nerve sent a branch to the biceps brachii muscle, the medial antebrachial cutaneous nerve, and a branch to the brachialis muscle. This case report may provide clues to understanding the phylogeny and embryology of the brachial plexus in Canidae.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Nyampong, Miyuka Nishizato, Weiyin Hu, Hiroshi Shimoda, Daisuke Hayasaka
{"title":"Temperature-dependent infectious stability of Severe fever with thrombocytopenia syndrome virus.","authors":"Samuel Nyampong, Miyuka Nishizato, Weiyin Hu, Hiroshi Shimoda, Daisuke Hayasaka","doi":"10.1292/jvms.26-0047","DOIUrl":"https://doi.org/10.1292/jvms.26-0047","url":null,"abstract":"<p><p>Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne zoonotic pathogen of increasing concern in East Asia, including Japan. Not only humans but also domestic cats are highly susceptible and frequently develop severe disease. Despite its public and veterinary health significance, data on the environmental stability of SFTSV remain limited. In this study, we evaluated the stability profiles of SFTSV under various temperature and inactivation conditions. As comparative controls, Kabuto Mountain virus (KMV) which belongs to the same class and order as SFTSV, Japanese Encephalitis virus (JEV) representing a different type of enveloped virus, and Feline Calicivirus (FCV) representing an example of non-enveloped virus, were also tested. SFTSV showed higher infectious stability at 4°C, 23°C and 37°C compared to KMV, JEV and FCV. In addition, SFTSV demonstrated comparatively higher resistance to heat inactivation at 56°C and 94°C. On the other hand, SFTSV was easily inactivated by ethanol, hypochlorite and ultraviolet irradiation. These findings provide important insights into the environmental persistence and inactivation requirements of SFTSV, suggesting that appropriate inactivation procedures are important to reduce the risk of SFTSV infection from the contaminated samples at clinical sites and laboratories.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ho To, Kaho Teshima, Nobuyuki Tsutsumi, Shinya Nagai, Katsuaki Sugiura
{"title":"Application of an enzyme-linked immunosorbent assay to detect antibodies to Actinobacillus pleuropneumoniae serovars 1, 2 and 5 in the pig sera.","authors":"Ho To, Kaho Teshima, Nobuyuki Tsutsumi, Shinya Nagai, Katsuaki Sugiura","doi":"10.1292/jvms.25-0472","DOIUrl":"https://doi.org/10.1292/jvms.25-0472","url":null,"abstract":"<p><p>An indirect and a sandwich enzyme-linked immunosorbent assay (ELISA), employing long-chain lipopolysaccharides (LC-LPS) and crude boiled extract as antigens respectively, were evaluated for the detection of antibodies against Actinobacillus pleuropneumoniae serovars 1, 2, 5, 9 and 11 in pig sera. By testing sera from pigs experimentally infected with the 10 recognized serovars, both assays were proven to have comparable sensitivity and specificity. With sera from pigs immunized with vaccines containing antigens of A. pleuropneumoniae serovars 1, 2 and 5, the assays were found to have a good correlation. The high diagnostic sensitivity and specificity of the indirect ELISA, along with its efficiency and ease of standardization-especially when using well-characterized antigens such as LC-LPS-may make it a valuable tool for field diagnostics.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of liquid nitrogen treatment on the mechanical properties of canine long bones.","authors":"Daisuke Yazawa, Nobuo Kanno, Shuji Suzuki, Takuya Yogo, Yasuji Harada, Yasushi Hara","doi":"10.1292/jvms.25-0525","DOIUrl":"https://doi.org/10.1292/jvms.25-0525","url":null,"abstract":"<p><p>We aimed to investigate the mechanical properties of canine long bones treated with liquid nitrogen for limb sparing surgery (LSS) as described by Tsuchiya et al. Three-point bending tests were performed on left and right femurs and tibias obtained from healthy beagle cadavers to evaluate the bone mechanical properties. There were no significant differences in mechanical properties between the left and right bones from the same individual. Furthermore, none of the indices differed between liquid nitrogen-treated and untreated bones. Our findings suggest that liquid nitrogen treatment does not result in marked changes in the mechanical properties of canine long bones, and may provide basic mechanical information potentially relevant to LSS using liquid nitrogen-treated autografts.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni Luh Ayu Megasari, Krisnoadi Rahardjo, Boedi Setiawan, Abhirama Zabdika Prasetya, I Wayan Andama Sindhuranu, Maria Inge Lusida, Yasuko Mori, Rima Ratnanggana Prasetya
{"title":"Molecular screening for active Severe Acute Respiratory Syndrome Coronavirus 2 infections among non-human primates in Indonesia.","authors":"Ni Luh Ayu Megasari, Krisnoadi Rahardjo, Boedi Setiawan, Abhirama Zabdika Prasetya, I Wayan Andama Sindhuranu, Maria Inge Lusida, Yasuko Mori, Rima Ratnanggana Prasetya","doi":"10.1292/jvms.25-0544","DOIUrl":"https://doi.org/10.1292/jvms.25-0544","url":null,"abstract":"<p><p>Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses an ongoing public health concern, and its zoonotic nature increases the risk of cross-species transmission between humans and animals. Non-human primates (NHPs) are particularly susceptible because of the high similarity in angiotensin-converting enzyme 2 (ACE2) critical residues compared to those in humans, especially among catarrhine species. Therefore, surveillance of NHP populations is essential, particularly in Indonesia, a megadiverse nation hosting numerous primate species that frequently interact with humans. In this study, 237 NHPs representing multiple species, including long-tailed macaques (Macaca fascicularis), langurs (Trachypithecus spp.), gibbons (Hylobates spp.), and orangutans (Pongo pygmaeus), were sampled from both in situ and ex situ conservation settings across five provinces (Bali, East Java, West Java, Central Kalimantan, and West Sumatra). Paired nasopharyngeal and oropharyngeal swabs were tested for SARS-CoV-2 RNA using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). No molecular evidence of active SARS-CoV-2 infection was detected in any of the individuals sampled. None of the samples showed amplification of either the N1 or N2 gene targets, and no borderline Ct values (all Ct >35 or undetermined) or single-target reactivity was observed. These findings highlight the importance of ongoing surveillance of Indonesian NHP populations by expanding geographical coverage, increasing the sample sizes, incorporating serological testing, and evaluating additional sample types to better assess potential spillover and spillback risks.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A canine case of multiple intrahepatic portosystemic shunts.","authors":"Shinichiro Yoda, Hideki Kayanuma, Takuya Maruo","doi":"10.1292/jvms.25-0550","DOIUrl":"10.1292/jvms.25-0550","url":null,"abstract":"<p><p>An elderly, spayed female Maltese dog (10 years 3 months, 2.2 kg) presented with hyporexia, weight loss, polydipsia, and a staggering gait. Serum biochemistry analysis revealed increased aspartate aminotransferase, alanine transaminase, ammonia (226 μg/dL), and fasting total bile acids (130 μmol/L). Contrast-enhanced CT showed multiple clusters of contrast enhancement within the hepatic parenchyma; however, discrete shunt vessels could not be clearly identified, and a definitive diagnosis could not be established based on CT alone. Intraoperative jejunal venous portography revealed numerous microvascular clusters connecting portal branches to the hepatic vein and caudal vena cava. The portal pressure was 7 mmHg. Based on these findings, multiple intrahepatic portosystemic shunts (MIPSS) were diagnosed, and medical management was selected.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"762-766"},"PeriodicalIF":1.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Developmental disorders of the reproductive tract in C57BL/6N-XY<sup>POS</sup> mice.","authors":"Honoka Kawai, Toshifumi Yokoyama, Chihiro Inuzuka, Sachika Kuramoto, Yaichi Hoshikawa, Youhei Mantani, Takanori Miki, Nobuhiko Hoshi","doi":"10.1292/jvms.26-0035","DOIUrl":"10.1292/jvms.26-0035","url":null,"abstract":"<p><p>Mammalian embryos possess undifferentiated gonads and primordial male and female reproductive tracts. In males, sex-determining region of the Y chromosome (Sry) triggers the differentiation of gonadal supporting cell precursors into Sertoli cells. In C57BL/6N-XY<sup>POS</sup> (B6N-XY<sup>POS</sup>) mice, a model of disorders of sex development, Sry expression is delayed relative to wild-type B6N mice, and several reproductive tract malformations have been reported. In this study, we investigated the developmental stage and regions at which reproductive tract development fails in B6N-XY<sup>POS</sup>. In B6N-XY<sup>POS</sup> at 15.5 days post-coitum (dpc), the number of seminiferous tubules showed no difference from B6N, but seminiferous tubules with closed at both ends appeared. In B6N-XY<sup>POS</sup> with bilaterally developed ovaries or testes, significant disorders were observed only in the female reproductive tract. B6N-XY<sup>POS</sup> ovotestes showed impaired testis differentiation in the cranial and caudal regions, not only in fetuses but also in adults. In female B6N-XY<sup>POS</sup> with bilaterally or unilaterally formed ovaries, the Müllerian ducts opened into the urogenital sinus at 15.5 dpc; however, at postnatal day 1, one individual exhibited a uterus with a closed region, and many adult females formed an edematous uterus. These results suggest that impaired cranial testis differentiation may disrupt the connection between the seminiferous tubules and the rete testis. They also suggest that Müllerian duct formation is initially completed even in female B6N-XY<sup>POS</sup> with an edematous uterus, but that the duct closes abnormally between 15.5 dpc and birth.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"746-755"},"PeriodicalIF":1.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minji Kim, Yubin Kim, Minji Kim, Junyoung Kim, Jongtae Cheong, Youngmin Yoon, Hyung-Kyu Chae, Woo-Jin Song, Myung-Chul Kim
{"title":"A case of suspected ectopic parathyroid chief cell carcinoma in a cat.","authors":"Minji Kim, Yubin Kim, Minji Kim, Junyoung Kim, Jongtae Cheong, Youngmin Yoon, Hyung-Kyu Chae, Woo-Jin Song, Myung-Chul Kim","doi":"10.1292/jvms.25-0480","DOIUrl":"10.1292/jvms.25-0480","url":null,"abstract":"<p><p>A 12-year-old spayed female Korean shorthair cat was evaluated for mediastinal and cervical masses. A serum biochemistry analysis revealed hypercalcemia and hypophosphatemia, with normal parathyroid hormone (PTH) levels. Diagnostic imaging showed multicystic cervical and mediastinal masses with increased opacity in the left caudal lung field, suggestive of partial lung collapse. Abdominal imaging was unremarkable. Cytology of the mediastinal mass suggested an epithelial or neuroendocrine tumor. Hypercalcemia remained unresolved following bisphosphonate therapy; the cat was euthanized 290 days after presentation. Postmortem examination revealed multicystic cervical and mediastinal masses with hemorrhage and necrosis. Histopathology showed neoplastic epithelial cells with cystic degeneration and lymphatic invasion in cervical and mediastinal masses. Immunohistochemistry confirmed PTH expression, establishing the chief cell parathyroid carcinoma. To our knowledge, this case represents a suspected ectopic feline parathyroid chief cell carcinoma with extensive cystic change and metastatic features.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"756-761"},"PeriodicalIF":1.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}