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Journal of Veterinary Medical Science最新文献

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A clinical case of enzootic bovine leukosis in a Holstein cow with minor clonality of B-cell in the peripheral blood. 一例荷斯坦奶牛感染牛白血病的临床病例，其外周血中的 B 细胞有轻微克隆现象。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-04 DOI: 10.1292/jvms.24-0298
Masaki Maezawa, Misato Kikuzawa, Asahi Takiguchi, Ryo Saito, James K Chambers, Kazuyuki Uchida, Hisashi Inokuma
{"title":"A clinical case of enzootic bovine leukosis in a Holstein cow with minor clonality of B-cell in the peripheral blood.","authors":"Masaki Maezawa, Misato Kikuzawa, Asahi Takiguchi, Ryo Saito, James K Chambers, Kazuyuki Uchida, Hisashi Inokuma","doi":"10.1292/jvms.24-0298","DOIUrl":"https://doi.org/10.1292/jvms.24-0298","url":null,"abstract":"<p><p>A 4-year 9-month-old Holstein-Friesian dairy cow presented with anorexia. On physical examination, swelling of superficial lymph nodes, pelvic masses, and prolonged urination posture after urinating a small amount were noted. Hematological examination revealed no lymphocytosis. The bovine leukemia virus proviral load was relatively high. At necropsy, enlarged lymph nodes, a large mass in the pelvic cavity, and mass lesions in several organs were observed. Histopathological examination revealed the proliferation of neoplastic lymphocytes, which were immune-positive for CD79α and negative for CD3. B-cell clonality test indicated the presence of monoclonality in the urine, masses, and lymph nodes and minor clonality in the peripheral blood. These findings led to a diagnosis of EBL with minor clonality of B-cell in the peripheral blood.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A clinical case of B-cell lymphoma in a Japanese Black cow with monoclonal proliferation of B-cells as revealed by polymerase chain reaction based on the immunoglobulin light chain gene. 基于免疫球蛋白轻链基因的聚合酶链反应揭示的日本黑牛 B 细胞淋巴瘤单克隆增殖临床病例。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-04 DOI: 10.1292/jvms.24-0320
Tatsuki Nagata, Toshiyuki Harada, Masaki Maezawa, James K Chambers, Kazuyuki Uchida, Hisashi Inokuma
{"title":"A clinical case of B-cell lymphoma in a Japanese Black cow with monoclonal proliferation of B-cells as revealed by polymerase chain reaction based on the immunoglobulin light chain gene.","authors":"Tatsuki Nagata, Toshiyuki Harada, Masaki Maezawa, James K Chambers, Kazuyuki Uchida, Hisashi Inokuma","doi":"10.1292/jvms.24-0320","DOIUrl":"https://doi.org/10.1292/jvms.24-0320","url":null,"abstract":"<p><p>A 35-month-old Japanese Black cow was presented to a local veterinarian with a main complaint of premature labor. There was no swelling of lymph nodes on the body surface and no palpable mass on rectal examination. Although atypical lymphocytes were observed in the peripheral blood, no lymphocytosis was recorded. On Day 12, the cow developed hindlimb paresis and was euthanized. Necropsy revealed swelling of some lymph nodes, and white nodules in uterus and right atrial appendage. Histopathological examination revealed B-cell lymphoma. In clonality analysis of peripheral blood and tumor tissue, monoclonal proliferation was detected by PCR for immunoglobulin light chain (IgL) λ, suggesting neoplastic proliferation of B-cell. IgL-PCR is thought to be a possible tool for diagnosis of B-cell lymphoma.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142574868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A case of hydronephrosis due to intrarenal ureteral obstruction in a Japanese Black calf. 日本黑小牛肾内输尿管梗阻导致肾积水的病例。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-27 DOI: 10.1292/jvms.24-0173
Reiichiro Sato, Takuya Hirai, Asmaa A Hegazy, Mutsumi Nakai, Yukiko Sato, Kazutaka Yamada, Hiroyuki Satoh, Adrian Steiner
{"title":"A case of hydronephrosis due to intrarenal ureteral obstruction in a Japanese Black calf.","authors":"Reiichiro Sato, Takuya Hirai, Asmaa A Hegazy, Mutsumi Nakai, Yukiko Sato, Kazutaka Yamada, Hiroyuki Satoh, Adrian Steiner","doi":"10.1292/jvms.24-0173","DOIUrl":"10.1292/jvms.24-0173","url":null,"abstract":"<p><p>A 23-day-old Japanese Black female calf presented with distension of the lower abdomen. Abdominal ultrasonography revealed a cystic structure with fluid accumulation and moderate echoluminance in the right abdominal cavity. Ultrasonography and contrast-enhanced computed tomography revealed congenital hydronephrosis due to narrowing of the intrarenal ureter, and right kidney resection was performed. Blood urea nitrogen and serum creatinine levels, which were within reference values preoperatively, peaked on the third postoperative day and gradually decreased afterward until reaching preoperative values on the tenth day. In bovine hydronephrosis, nephrectomy may provide a favorable prognosis if one kidney is intact. Moreover, this case suggests that blood tests immediately after surgery are not suitable for evaluating residual kidneys.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in antimicrobial resistance-related genes of Trueperella pyogenes between isolates detected from cattle and pigs. 从牛和猪中检测到的化脓性真菌抗菌药耐药性相关基因的差异。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-19 DOI: 10.1292/jvms.24-0180
Hideki Fujimoto, Natsuki Shimoji, Tatsumi Sunagawa, George Sanga, Takehisa Chuma
{"title":"Differences in antimicrobial resistance-related genes of Trueperella pyogenes between isolates detected from cattle and pigs.","authors":"Hideki Fujimoto, Natsuki Shimoji, Tatsumi Sunagawa, George Sanga, Takehisa Chuma","doi":"10.1292/jvms.24-0180","DOIUrl":"10.1292/jvms.24-0180","url":null,"abstract":"<p><p>We investigated antimicrobial resistance-related genes in 109 isolates of Trueperella pyogenes that were isolated in cattle and pigs. All 89 tetracycline-resistant T. pyogenes isolates carried the resistance gene harbored either tetW, tetM, tetA(33), tetK, or tetL. The ermX or ermB were detected in 18 of 23 erythromycin-resistant isolates. Streptomycin-resistant aadA1, aadA9, aadA11, aadA24, strA, or strB were detected in 25 of 83 isolates. There were significant differences in the percentages of tetA(33), ermB, aadA1, aadA9, aadA11, or aadA24 carriage between cattle and pig isolates. In addition, the Class 1 gene cassette was detected only in 17 cattle isolates. This suggests that T. pyogenes isolates acquire resistance gene in each environment of cattle and pigs, and that the transmission of the bacteria between cattle and pigs is limited.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Distribution of lesions in psittacine birds naturally infected with parrot bornavirus in Japan. 日本自然感染鹦鹉病毒的鹦鹉鸟的病变分布。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-27 DOI: 10.1292/jvms.24-0061
Chinatsu Fujiwara, Naoyuki Aihara, Motokazu Yoshino, Takanori Shiga, Kan Fujino, Ryo Komorizono, Akiko Makino, Keizo Tomonaga, Reiko Soga, Junichi Kamiie
{"title":"Distribution of lesions in psittacine birds naturally infected with parrot bornavirus in Japan.","authors":"Chinatsu Fujiwara, Naoyuki Aihara, Motokazu Yoshino, Takanori Shiga, Kan Fujino, Ryo Komorizono, Akiko Makino, Keizo Tomonaga, Reiko Soga, Junichi Kamiie","doi":"10.1292/jvms.24-0061","DOIUrl":"10.1292/jvms.24-0061","url":null,"abstract":"<p><p>Mononuclear cell infiltration of the central nervous system and ganglioneuritis are characteristic histopathological findings of proventricular dilatation disease (PDD) caused by parrot bornavirus (PaBV) infection. The purpose of this study was to clarify the link between the degree of inflammatory lesions and the distribution of the virus antigen in naturally PaBV-infected parrots. Pathological examination was performed on 18 PaBV-infected birds identified by reverse transcriptase-PCR. Dilatation of the crop, proventriculus, and ventriculus was observed in all 18 (100%) birds, and dilation of the right ventricle of the heart was observed in 14/18 (78%) birds. Cases were classified based on the scores for the distribution and degree of histological lesions into neural type, with severe brain lesions, digestive type, with severe gastric lesions, or nervous/digestive type, with severe lesions in both the brain and ventriculus. The PaBV immunohistological score correlated with the inflammatory lesion scores. Ganglioneuritis, myocarditis, and myocardial degeneration were frequently observed in the heart. Interestingly, macroscopic and microscopic lesions and virus antigen were detected in the hearts of all three histological types. The present study showed that parrots naturally infected with PaBVs can be grouped into three types based on the lesion distribution, and heart failure is an important symptom in PaBV-infected parrots.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preclinical/clinical trials of thrice-weekly administration of a combination of tegafur/gimeracil/oteracil (TS-1) and toceranib phosphate in dogs with intranasal tumors. 对患有鼻内肿瘤的狗每周三次服用替加氟/吉莫拉嘧啶/奥替拉西尔（TS-1）和磷酸托塞拉尼组合物的临床前/临床试验。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-10-03 DOI: 10.1292/jvms.23-0455
Yuta Nishiyama, Takuya Maruo, Yasuhiro Fukuyama, Yuka Odaka, Eiyu Kawata, Hirona Ueno, Hideki Kayanuma, Tomohiro Nakayama, Hiroki Takahashi
{"title":"Preclinical/clinical trials of thrice-weekly administration of a combination of tegafur/gimeracil/oteracil (TS-1) and toceranib phosphate in dogs with intranasal tumors.","authors":"Yuta Nishiyama, Takuya Maruo, Yasuhiro Fukuyama, Yuka Odaka, Eiyu Kawata, Hirona Ueno, Hideki Kayanuma, Tomohiro Nakayama, Hiroki Takahashi","doi":"10.1292/jvms.23-0455","DOIUrl":"10.1292/jvms.23-0455","url":null,"abstract":"<p><p>Intranasal tumors in dogs are malignant solid tumors that are primarily treated with radiotherapy and often recur post-treatment. Combination therapy is pivotal in cancer therapy. Effective drugs include fluoropyrimidine 5-fluorouracil (5-FU) and toceranib phosphate. TS-1, an oral formulation containing the 5-FU prodrug tegafur and enzyme modulators gimeracil and oteracil, is proven to be safe in dogs with solid tumors. While the oral drug toceranib phosphate (Palladia<sup>®</sup>) is safely administered, the combined toxicity with TS-1 is unknown. We aimed to determine the dosage of this combination in dogs. In the preclinical/clinical trials conducted here, we used a standard 3+3 cohort design with fixed doses of toceranib phosphate (2.4 mg/kg) administered thrice weekly. TS-1 administration was initiated at a dose of 0.5 mg/kg (upper limit 2.0 mg/kg) thrice weekly. Four cohorts were included to confirm the safety of TS-1 and toceranib phosphate. Each cohort was followed up for 1 month. The intranasal tumor types included in the clinical trial (n=13) were adenocarcinoma (n=7), squamous cell carcinoma (n=1), non-epithelial malignancy (n=2), undifferentiated carcinoma (n=1), and transitional carcinoma (n=2). The TS-1 dosage could be increased up to its dose limit in the preclinical/clinical trials. The TS-1 dose to combine with toceranib phosphate thrice weekly was 2.0 mg/kg. This regimen was well-tolerated in dogs. Thus, combined TS-1 and toceranib phosphate therapy is safe for dogs with intranasal tumors.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells. 针对肉豆蔻酰化富丙氨酸 C 激酶底物 N 末端的多肽可促进 SH-SY5Y 人神经母细胞瘤细胞的神经元分化。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-27 DOI: 10.1292/jvms.24-0276
Jannatul Ferdous, Kiyotada Naitou, Mitsuya Shiraishi
{"title":"A peptide against the N-terminus of myristoylated alanine-rich C kinase substrate promotes neuronal differentiation in SH-SY5Y human neuroblastoma cells.","authors":"Jannatul Ferdous, Kiyotada Naitou, Mitsuya Shiraishi","doi":"10.1292/jvms.24-0276","DOIUrl":"10.1292/jvms.24-0276","url":null,"abstract":"<p><p>Myristoylated alanine-rich protein kinase C substrate (MARCKS) plays crucial roles in neuronal functions and differentiation. However, specific effects of the myristoylated N-terminal sequence (MANS) peptide, a widely used MARCKS modulator comprising the initial 24 amino acids of MARCKS, on neuronal cells remain unclear. Therefore, in this study, we aimed to examine the effects and action mechanisms of the MANS peptide on SH-SY5Y human neuroblastoma cells, which served as the in vitro neuronal cell models. MANS treatment of SH-SY5Y cells resulted in significant neurite outgrowth within 24 hr, which was as prominent as that induced by seven days of treatment with all-trans retinoic acid, the most common agent used to induce SH-SY5Y cell differentiation. Levels of synaptophysin, a neuronal marker protein, were significantly increased in the MANS peptide-treated cells. Additionally, increased MARCKS levels and decreased MARCKS phosphorylation were observed in MANS peptide-treated cells. Notably, neurite outgrowth induced by the MANS peptide was significantly reduced in MARCKS-knocked-down cells. Overall, these results suggest the MANS peptide as a novel agent for SH-SY5Y cell differentiation, particularly for the analysis of MARCKS functions.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A case of urachal abscess resection via colpotomy in a cow. 一例通过结肠切除术切除尿道脓肿的奶牛。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-17 DOI: 10.1292/jvms.24-0256
Reiichiro Sato, Yukiko Sato, Naoyuki Fuke, Ryoko Uemura, Adrian Steiner
{"title":"A case of urachal abscess resection via colpotomy in a cow.","authors":"Reiichiro Sato, Yukiko Sato, Naoyuki Fuke, Ryoko Uemura, Adrian Steiner","doi":"10.1292/jvms.24-0256","DOIUrl":"10.1292/jvms.24-0256","url":null,"abstract":"<p><p>This study describes a novel surgical method to remove urinary bladder-related urachal abscesses in a cow. Traditional methods, such as the midline or paramedian approach in calves and the preinguinal approach, can be challenging in adult cattle, especially for lesions situated near the pelvic cavity. In this case, a 4-year-old cow had a urachal abscess attached to the bladder apex, making it difficult to approach via abdominal wall incision. Colpotomy and pulling the abscess through a vaginal wall incision allowed for complete exteriorization of the lesion and comfortable accessibility of the abscess and the resection site. This report suggests that colpotomy is an effective new route in adult cattle for accessing urachal abscesses not accessible via traditional surgical approaches.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The anti-inflammatory effects of Fuzapladib in an endotoxemic porcine model. 福扎普拉迪布在猪内毒素模型中的抗炎作用
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-12 DOI: 10.1292/jvms.24-0190
Chihiro Sugita, Takaharu Itami, Taku Miyasho, I-Ying Chen, Taku Hirokawa, Haruki Tsukui, Miki Kato, Marin Shibuya, Yuto Sano, Keiko Kato, Kazuto Yamashita
{"title":"The anti-inflammatory effects of Fuzapladib in an endotoxemic porcine model.","authors":"Chihiro Sugita, Takaharu Itami, Taku Miyasho, I-Ying Chen, Taku Hirokawa, Haruki Tsukui, Miki Kato, Marin Shibuya, Yuto Sano, Keiko Kato, Kazuto Yamashita","doi":"10.1292/jvms.24-0190","DOIUrl":"10.1292/jvms.24-0190","url":null,"abstract":"<p><p>Endotoxemia is a systemic inflammatory condition caused by lipopolysaccharide (LPS) stimulation, which produces inflammatory cytokines. Fuzapladib (FZP) inhibits the activation of adhesion molecules found on the surface of inflammatory cells, mitigating inflammation. In this study, we evaluated the therapeutic effects of fuzapladib on inflammatory cytokines and cardio-respiratory function using an LPS-induced endotoxemic porcine model. Fifteen pigs were separated into three groups: low-FZP (n=5), high-FZP (n=5), and control (n=5). Pigs were administered LPS under general anesthesia, and complete blood cell count, blood biochemistry, inflammatory cytokines, and cardio-respiratory function were evaluated. Statistical analysis was performed using a linear mixed-effects model and the Steel-Dwass test, with a significance threshold of P<0.05. During the 4 hr experimental period, one pig in the control group and two pigs in the low-FZP group died due to hypoxemia and hypotension. In the early acute changes following LPS administration, the high-FZP group maintained significantly higher arterial oxygen partial pressure and normal blood pressure compared to the control group. Although interleukin-6 levels increased in all groups during the experiment, they were significantly lower in the high-FZP group compared to the control group. Other parameters showed no clinically significant differences. In conclusion, while high-dose fuzapladib did not reduce organ damage in the porcine endotoxemia model, it suppressed interleukin-6 production, delayed the progression of deterioration, and contributed to a reduction in mortality during the observation period.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of DNase I in DNA degradation and cell-free DNA generation after acetaminophen-induced hepatic injury. DNase I 在对乙酰氨基酚引起的肝损伤后 DNA 降解和游离 DNA 生成中的作用
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-11-01 Epub Date: 2024-09-18 DOI: 10.1292/jvms.23-0344
Shuhei Takada, Shuhei Ogawa, Ryushin Mizuta
{"title":"Role of DNase I in DNA degradation and cell-free DNA generation after acetaminophen-induced hepatic injury.","authors":"Shuhei Takada, Shuhei Ogawa, Ryushin Mizuta","doi":"10.1292/jvms.23-0344","DOIUrl":"10.1292/jvms.23-0344","url":null,"abstract":"<p><p>Cell-free DNA (cfDNA), the DNA in the blood circulation, is a useful marker for diagnosing hereditary diseases and tumors. However, the mechanisms underlying the generation of cfDNA are not completely understood. We previously studied DNases [Caspase-activated DNase (CAD), DNase1L3, and DNase I] and reported that in acetaminophen-induced liver necrosis, DNase1L3 was the main endonuclease generating cfDNA, with CAD playing a supporting role. In this study, we generated triple-gene knockout (TKO) mice, Cad<sup>-/-</sup>DNase1L3<sup>-/-</sup>DNase1<sup>-/-</sup>, and found that DNase I also contributed to cfDNA generation. Given that a defect in DNase1L3 or DNase I is involved in autoimmune diseases, TKO mice would be useful as a disease model and tool for identifying the in vivo roles of endonucleases.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142299677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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