Norio Yamagishi, Khaing Shwe Sin Soe, Hijiri Suyama, Akari Kawamura, Shinobu Tsuchiaka, Kouki Itagaki, Sueun Kim, Shingo Ishikawa
{"title":"两种用于牛血清蛋白分离的琼脂糖凝胶电泳试剂盒的评价。","authors":"Norio Yamagishi, Khaing Shwe Sin Soe, Hijiri Suyama, Akari Kawamura, Shinobu Tsuchiaka, Kouki Itagaki, Sueun Kim, Shingo Ishikawa","doi":"10.1292/jvms.25-0272","DOIUrl":null,"url":null,"abstract":"<p><p>We evaluated two human diagnostic agarose gel electrophoresis (AGE) kits (J711 and J718) for bovine serum protein fractionation using 79 bovine samples, comparing the results with a biochemistry analyzer (BA). While six protein fractions were commonly observed, β<sub>2</sub>- and γ-globulin (Glb) fusion (β<sub>2</sub>γ-Glb) occurred in some inflammatory and neonatal cases. The results using both AGE kits correlated with those of the BA for total albumin and Glb, with J711 showing superior agreement. However, inter-kit variability was observed for individual protein fractions, particularly α<sub>1</sub>-, α<sub>2</sub>-, and β<sub>1</sub>-Glb, which showed proportional or constant errors and moderate-to-poor reliability, despite good agreement for β<sub>2</sub>γ-Glb. These findings necessitate caution in interpreting fraction-specific results and underscore the importance of stating the AGE kit in veterinary diagnostics.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of two human diagnostic agarose gel electrophoresis kits for bovine serum protein fractionation.\",\"authors\":\"Norio Yamagishi, Khaing Shwe Sin Soe, Hijiri Suyama, Akari Kawamura, Shinobu Tsuchiaka, Kouki Itagaki, Sueun Kim, Shingo Ishikawa\",\"doi\":\"10.1292/jvms.25-0272\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We evaluated two human diagnostic agarose gel electrophoresis (AGE) kits (J711 and J718) for bovine serum protein fractionation using 79 bovine samples, comparing the results with a biochemistry analyzer (BA). While six protein fractions were commonly observed, β<sub>2</sub>- and γ-globulin (Glb) fusion (β<sub>2</sub>γ-Glb) occurred in some inflammatory and neonatal cases. The results using both AGE kits correlated with those of the BA for total albumin and Glb, with J711 showing superior agreement. However, inter-kit variability was observed for individual protein fractions, particularly α<sub>1</sub>-, α<sub>2</sub>-, and β<sub>1</sub>-Glb, which showed proportional or constant errors and moderate-to-poor reliability, despite good agreement for β<sub>2</sub>γ-Glb. These findings necessitate caution in interpreting fraction-specific results and underscore the importance of stating the AGE kit in veterinary diagnostics.</p>\",\"PeriodicalId\":49959,\"journal\":{\"name\":\"Journal of Veterinary Medical Science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medical Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1292/jvms.25-0272\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.25-0272","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Evaluation of two human diagnostic agarose gel electrophoresis kits for bovine serum protein fractionation.
We evaluated two human diagnostic agarose gel electrophoresis (AGE) kits (J711 and J718) for bovine serum protein fractionation using 79 bovine samples, comparing the results with a biochemistry analyzer (BA). While six protein fractions were commonly observed, β2- and γ-globulin (Glb) fusion (β2γ-Glb) occurred in some inflammatory and neonatal cases. The results using both AGE kits correlated with those of the BA for total albumin and Glb, with J711 showing superior agreement. However, inter-kit variability was observed for individual protein fractions, particularly α1-, α2-, and β1-Glb, which showed proportional or constant errors and moderate-to-poor reliability, despite good agreement for β2γ-Glb. These findings necessitate caution in interpreting fraction-specific results and underscore the importance of stating the AGE kit in veterinary diagnostics.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
