Journal of Veterinary Medical Science最新文献_第2页

Development of a conformation-specific nanobody-based ELISA for quantitative detection of intact Senecavirus A virions and virus-like particles. 基于纳米体的完整塞内卡病毒a病毒粒子和病毒样颗粒定量检测方法的建立

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-02 Epub Date: 2026-03-23 DOI: 10.1292/jvms.25-0519

Yun Zhang, Songjia Pan, Suyu Mu, Jinen Wu, Tian Wei, Shiqi Sun, Huichen Guo

{"title":"Development of a conformation-specific nanobody-based ELISA for quantitative detection of intact Senecavirus A virions and virus-like particles.","authors":"Yun Zhang, Songjia Pan, Suyu Mu, Jinen Wu, Tian Wei, Shiqi Sun, Huichen Guo","doi":"10.1292/jvms.25-0519","DOIUrl":"10.1292/jvms.25-0519","url":null,"abstract":"Replication of Senecavirus A (SVA) generates intact virions and empty capsids, some of which disassemble into pentamers. To enable precise quality control of SVA intact viral antigen, we established a double-antibody sandwich ELISA to quantify intact SVA virions and virus-like particles (VLPs). The assay employs a conformation-specific nanobody (SV1) as the capture antibody, which binds both virions and VLPs, and a His-tagged nanobody (SVH1) as the detection antibody. Using purified virions as standards, the assay demonstrated a limit of detection (LOD) of 9.7 ng/mL, with a linear range spanning from 9.7 ng/mL to 1.25 μg/mL. Critically, it showed no cross-reactivity with pentamers and other viral particles, including foot-and-mouth disease virus (FMDV), porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), classical swine fever virus (CSFV), and encephalomyocarditis virus (EMCV). The coefficients of variation (CV) were <10% for both intra-batch and inter-batch analyses. Validation against sucrose density gradient centrifugation (SDGC) confirmed concordant results. Applied to vaccine development, the ELISA quantified a 36% antigen loss in SVA VLPs after emulsification. In summary, this method provides a rapid, specific tool for SVA vaccine standardization.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"767-773"},"PeriodicalIF":1.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147500221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 2025 outbreak of equine influenza among draft horses at Obihiro Racecourse, Hokkaido, Japan. 2025年在日本北海道大宽赛马场的牵马中爆发的马流感。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-02 Epub Date: 2026-03-25 DOI: 10.1292/jvms.26-0092

Natsuko Fukumoto, Makoto Shibata, Mayu Hayashi, Kyoko Kawauchi, Yukiko Yabuuchi, Yasuhiro Oue, Ryota Matsuyama, Takehisa Yamamoto, Hiroshi Mita, Nanako Kawanishi, Hiroshi Bannai, Koji Tsujimura, Takashi Yamanaka, Manabu Nemoto

引用次数: 0

Conservative management of cranial cruciate ligament injury in an Asian small-clawed otter (Aonyx cinereus). 亚洲小爪水獭（Aonyx cinereus）颅十字韧带损伤的保守治疗。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-02 Epub Date: 2026-03-31 DOI: 10.1292/jvms.25-0538

Mizuki Tomihari, Shoki Murakami, Toshiyuki Tanaka, Takashi Hasegawa

引用次数: 0

Evaluation of the efficacy and safety of a modified anesthetic protocol using dexmedetomidine, midazolam, and butorphanol in mice. 右美托咪定、咪达唑仑和布托啡诺对小鼠改良麻醉方案的有效性和安全性评价。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-18 DOI: 10.1292/jvms.25-0568

Natsuki Kida, Haruto Takeda, Kazuhiro Sakamoto, Masaki Watanabe, Nobuya Sasaki, Atsushi Asano

{"title":"Evaluation of the efficacy and safety of a modified anesthetic protocol using dexmedetomidine, midazolam, and butorphanol in mice.","authors":"Natsuki Kida, Haruto Takeda, Kazuhiro Sakamoto, Masaki Watanabe, Nobuya Sasaki, Atsushi Asano","doi":"10.1292/jvms.25-0568","DOIUrl":"10.1292/jvms.25-0568","url":null,"abstract":"We aimed to evaluate the efficacy and safety of a modified injectable anesthesia protocol combining dexmedetomidine, midazolam, and butorphanol (dMMB) compared with the conventional medetomidine-based protocol (MMB) in C57BL/6J mice. Animals were assigned to three groups (n=5 per group for each sex): MMB (0.5/4.0/5.0), dMMB (0.25/4.0/5.0), and dMMB (0.1/6.0/10.0) mg/kg. We assessed anesthetic induction and recovery times, depth scores, heart rate, SpO2, and body temperature, and administered atipamezole 40 min post-anesthesia to evaluate temperature recovery. Blood glucose levels were also measured. Both dMMB protocols produced anesthetic effects comparable to MMB, but recovery was significantly faster than MMB. Duration of surgical anesthesia in males (mean ± 95% confidence interval) was 141 ± 11 min for MMB, 112 ± 8 min for dMMB (0.25/4.0/5.0), and 89 ± 10 min for dMMB (0.1/6.0/10.0). In females, durations were 109 ± 43 min, 76 ± 12 min, and 78 ± 16 min, respectively. In addition, hypothermia was less severe, particularly with the lowest dexmedetomidine dose (0.1/6.0/10.0). Furthermore, females had a significantly shorter duration of surgical anesthesia than males. Blood glucose levels increased under all anesthetic protocols, with males showing higher values than females, while no significant differences among anesthetic protocols were detected in either sex. No additional adverse effects were observed. These findings indicated that low-dose dexmedetomidine-based anesthesia provides effective sedation while improving safety by reducing hypothermia and facilitating rapid recovery.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"694-703"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147482171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravenous administration of a selective metabotropic glutamate receptor 2/3 agonist attenuates hypertension in spontaneously hypertensive rats. 静脉注射选择性代谢性谷氨酸受体2/3激动剂可减轻自发性高血压大鼠的高血压。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-10 DOI: 10.1292/jvms.26-0051

Suzu Aoki, Yoshiyasu Nagashima, Ryota Tochinai, Shin-Ichi Sekizawa

{"title":"Intravenous administration of a selective metabotropic glutamate receptor 2/3 agonist attenuates hypertension in spontaneously hypertensive rats.","authors":"Suzu Aoki, Yoshiyasu Nagashima, Ryota Tochinai, Shin-Ichi Sekizawa","doi":"10.1292/jvms.26-0051","DOIUrl":"10.1292/jvms.26-0051","url":null,"abstract":"Hypertension is associated with impaired central autonomic regulation and remains a major risk factor for cardiovascular diseases. The nucleus tractus solitarius (NTS) in the medulla oblongata plays a critical role in blood pressure control via glutamatergic neurotransmission from peripheral baroreceptors. Previous studies have shown that intracisternal administration of Group II metabotropic glutamate receptor (mGluR2/3) agonists attenuates hypertension; however, the clinical applicability of this invasive approach is limited. The present study investigated whether systemic administration of a selective mGluR2/3 agonist, LY 379268, can modulate central blood pressure regulation and attenuate hypertension. LY 379268 was administered intravenously to spontaneously hypertensive rats (SHRs). In anesthetized conditions, arterial blood pressure and heart rate were continuously recorded and dose-response effects were evaluated. In conscious conditions, LY 379268 were administered into the tail vein of control SHRs or SHRs with intact (Sham) or preoperatively transected baroreceptor information (Sino-Aortic Denervation, SAD), and blood pressure was periodically measured by the tail-cuff method. A dose-dependent reduction in arterial blood pressure without significant changes in heart rate was observed up to moderate doses (10 μg/kg), showing bell-shape response. Similarly, aforementioned dose of LY 379268 i.v. injection decreased blood pressure in control or Sham-SHRs but not in SAD-SHRs in conscious condition. In addition, intravenous Evans blue injection demonstrated increased permeability of the blood-brain-barrier in the NTS. These findings indicate that systemic activation of mGluR2/3 can attenuate hypertension by modulating central autonomic regulation via access to the NTS. Intravenous administration of mGluR2/3 agonists may represent a minimally invasive strategy for targeting central mechanisms underlying hypertension.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"719-727"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147436909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The nurturing ability of PVG-RT7b rats is strongly affected by the fetal environment. PVG-RT7b大鼠的育性能力受胎儿环境的强烈影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-06 DOI: 10.1292/jvms.25-0463

Hiroe Kon, Hisashi Ueta

{"title":"The nurturing ability of PVG-RT7b rats is strongly affected by the fetal environment.","authors":"Hiroe Kon, Hisashi Ueta","doi":"10.1292/jvms.25-0463","DOIUrl":"10.1292/jvms.25-0463","url":null,"abstract":"Our strain of inbred PVG-RT7b (PVG.7b) rats was at risk of failing to be maintained owing to frequent neonatal death. Only 23.5% of mothers succeeded in nurturing their offspring at the first parturition. Furthermore, mothers failing to nurture their offspring at first parturition also failed in the second one. Here, we investigated the cause of frequent neonatal deaths in PVG.7b rats. Exchanging newborn offspring between PVG.7b and Wistar-Imamichi (WI) rats. resulted in 80.0% of WI mothers succeeding in nurturing PVG.7b offspring, whereas only 18.2% of PVG.7b mothers succeeded in nurturing WI offspring. These findings indicate that the neonatal mortality of PVG.7b is due to maternal factors rather than inherent offspring issues. Additionally, we examined the mammary glands of PVG.7b immediately after the second parturition. PVG.7b rats, which initially failed to nurture, exhibited normal mammary gland development and milk production after the second birth. These results suggested that neonatal death is due to maternal behavior. Moreover, we investigated the nurturing ability of PVG.7b rats nurtured by WI foster mothers (PVG.7b Infant/WI) or born and nurtured from WI recipients after embryo transfer (PVG.7b ET/WI). Only 26.1% of PVG.7b Infant/WI mothers succeeded in nurturing their offspring, compared to 63.6% of PVG.7b ET/WI mothers. However, only 20.0% of PVG.7b mothers born and nursed by PVG.7b ET/WI succeeded in nurturing their offspring. These results suggest that the fetal environment influences the nurturing ability of PVG.7b rats.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"687-693"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Squamous cell carcinoma in a captive Sunda pangolin (Manis javanica): clinical management and pathological findings. 圈养巽他穿山甲鳞状细胞癌：临床处理和病理结果。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-24 DOI: 10.1292/jvms.25-0504

Shin Min Chong, Charlene Yen-Feng Yeong, Ali Anwar Ahmad, Yirui Heng

引用次数: 0

A feline case of intestinal small T-cell lymphoma with prominent lymphoid follicle formation and concurrent intestinal large B-cell lymphoma. 猫肠小t细胞淋巴瘤伴显著淋巴滤泡形成并发肠大b细胞淋巴瘤1例。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-11 DOI: 10.1292/jvms.25-0458

Kosei Sakai, Tamiko Fumimoto, Masaru Furuya, Shunsuke Shimamura, Takeshi Izawa

引用次数: 0

Genetic analysis of bovine papular stomatitis and pseudocowpox viruses circulating in Hokkaido, Japan's largest cattle-producing region. 在日本最大的养牛区北海道流行的牛丘疹性口炎和假牛痘病毒的遗传分析。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-19 DOI: 10.1292/jvms.26-0001

Ayaka Yoshino, Ayaka Okada, Yasuo Inoshima

{"title":"Genetic analysis of bovine papular stomatitis and pseudocowpox viruses circulating in Hokkaido, Japan's largest cattle-producing region.","authors":"Ayaka Yoshino, Ayaka Okada, Yasuo Inoshima","doi":"10.1292/jvms.26-0001","DOIUrl":"10.1292/jvms.26-0001","url":null,"abstract":"Parapoxvirus infections caused by bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV) are globally distributed zoonoses in ruminants. Although parapoxviruses have been isolated and genetically characterized in several regions of Japan, no sequence data are available from Hokkaido, which has the country's largest cattle population. Consequently, the genetic characteristics of strains circulating in Hokkaido remain unclear. This study analyzed the genetic relationships and molecular epidemiology of 11 BPSV and two PCPV strains isolated in Hokkaido between 2005 and 2022. Full-length sequences of open reading frames (ORF) 11 and 32 were determined and compared with previously reported strains. The deduced amino acid identities of ORF11 ranged from 95 to 100% among 11 BPSV strains and 98% between the two PCPV strains. ORF32 amino acid identities among BPSV strains ranged from 88% to 100%. This study reports the first ORF32 sequences of Japanese parapoxviruses. Phylogenetic analysis based on ORF11 showed that 10 BPSV strains formed a single cluster, whereas one strain was distinct; the two PCPV strains also formed separate clusters. ORF32 analysis indicated that most BPSV strains clustered together, except for one strain with an amino acid deletion. Comparisons with domestic and foreign strains demonstrated that ORF11 is highly conserved, whereas ORF32 exhibits greater genetic diversity, supporting its suitability for molecular epidemiological analysis. These findings indicate that genetically related parapoxviruses are circulating in Hokkaido.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"728-734"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147482077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surveillance of gastrointestinal parasites in pheasants reared at farms and zoos in Japan: High prevalence of Eimeria species infection. 日本农场和动物园饲养的野鸡胃肠道寄生虫监测：艾美耳亚种感染高发。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-05-01 Epub Date: 2026-03-10 DOI: 10.1292/jvms.26-0009

Makoto Matsubayashi, Sayaka Tsuchida, Atsushi Kobayashi, Kazunari Ushida, Tomoyuki Shibahara

引用次数: 0