Journal of Veterinary Medical Science最新文献_第2页

Assessment of gelatin-epigallocatechin gallate nanoparticles with hyaluronic acid coating for treating dry eye associated keratopathy in rats.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-10 DOI: 10.1292/jvms.24-0250

Man-Ha Chan, Ching-Li Tseng, Wei-Hsiang Huang, Chung-Tien Lin

{"title":"Assessment of gelatin-epigallocatechin gallate nanoparticles with hyaluronic acid coating for treating dry eye associated keratopathy in rats.","authors":"Man-Ha Chan, Ching-Li Tseng, Wei-Hsiang Huang, Chung-Tien Lin","doi":"10.1292/jvms.24-0250","DOIUrl":"https://doi.org/10.1292/jvms.24-0250","url":null,"abstract":"Keratoconjunctivitis sicca (KCS) is a common ocular disease in dogs characterized by inflammation of the cornea and conjunctiva. This study investigates the effectiveness of gelatin-epigallocatechin gallate nanoparticles with hyaluronic acid coating (GEH NPs) and various artificial tears in treating KCS led keratopathy in a rat model. Eighteen female Sprague-Dawley rats, aged 6 to 8 weeks were randomly assigned to 6 groups of 3. All rats except the Sham group underwent unilateral exorbital and infraorbital lacrimal gland excisions to induce KCS. The treatment groups included a KCS group without treatment, a GEH group treated with GEH NPs, and 3 groups receiving different artificial tears. Clinical examinations, including slit-lamp biomicroscopy, optical coherence tomography (OCT), and ocular surface analyser-VET (OSA-VET) were conducted preoperatively at 1 and 2 weeks postoperatively. For treating KCS associated keratopathy, the GEH NPs showed superior outcomes and consistently ranked among the strongest treatment options in most aspects evaluated, including reduced corneal irregularity, improved tear film stability, and lower fluorescein scores, indicating better corneal integrity. Additionally, the GEH group displayed the least inflammatory cell infiltration and maintained a healthier epithelial structure, further underscoring its protective effects. GEH was administered twice daily while other treatment groups were administrated 3 times daily, highlighting its efficacy even with a lower dosing frequency. The artificial tears demonstrated variable benefits on KCS led keratopathy across different evaluations. GEH NPs exhibited excellent protective effects and therapeutic potential on dry eye associated keratopathy based on this study.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suspected fibrocartilaginous embolus in Asian small-clawed otter (Aonyx cinereus).

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-07 DOI: 10.1292/jvms.24-0462

Toshiyuki Tanaka, Konomi Ito, Yoshimi Miyagawa, Motomu Morishita, Mizuki Tomihari, Takashi Hasegawa

引用次数: 0

Treatment of esophagogastric intussusception due to cryptosporidiosis using paromomycin in a corn snake (Pantherophis guttatus).

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-07 DOI: 10.1292/jvms.24-0483

Urarako Shimizu, Manabu Kurihara, Kenichi Tamukai, Kazutaka Yamada

引用次数: 0

Upregulation of lipocalin-2 in the retina of experimental autoimmune uveitis.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 DOI: 10.1292/jvms.24-0231

Hyeonju Kim, Meejung Ahn, Kyungsook Jung, Sungmoo Hong, Deokho Ko, Taeyoung Kang, Taekyun Shin, Jeongtae Kim

引用次数: 0

Phosphorylation state of Akt in the heart during artificial deep hypothermia in Syrian hamsters. 人工深度低温下叙利亚仓鼠心脏Akt磷酸化状态的研究。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2024-12-31 DOI: 10.1292/jvms.24-0369

Yuuki Horii, Manami Kurata, Yuma Yoshida, Hiroki Sakai, Takahiko Shiina, Yasutake Shimizu

{"title":"Phosphorylation state of Akt in the heart during artificial deep hypothermia in Syrian hamsters.","authors":"Yuuki Horii, Manami Kurata, Yuma Yoshida, Hiroki Sakai, Takahiko Shiina, Yasutake Shimizu","doi":"10.1292/jvms.24-0369","DOIUrl":"10.1292/jvms.24-0369","url":null,"abstract":"Hibernating animals show a remarkable decrease in body temperature without accompanying serious organ damage. Active hypometabolism may be involved in the protective mechanisms. Therefore, in the present study, the phosphorylation status of Akt was used to examine whether metabolism is actively reduced during artificial hypothermia in hamsters. Hypothermia induced by activation of the central adenosine A1 receptor (A1AR) resulted in dephosphorylation of Akt in the heart, as in that of hibernating hamsters. Since phosphorylated Akt almost totally disappeared, it is probable that cellular metabolism is actively reduced in A1AR-mediated hypothermia. Surprisingly, phosphorylation of Akt was significantly, but not totally, reduced even when hypothermia was induced by a combination of inhalation anesthesia and cooling. It can thus be considered that active regulation for the reduction of metabolic activity occurs in A1AR-mediated hypothermia as well as in anesthesia-induced hypothermia, though to a lesser extent. In both hypothermic conditions, serious arrhythmias were rarely observed. In anesthesia-induced hypothermia, during which dephosphorylation of Akt occurred only partially, there was no obvious organ damage in histopathological examination. Taken together, our findings suggest that artificial hypothermia causes active hypometabolism and has therapeutically applicable properties.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"160-166"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing. 应用RNA测序技术分析醋酸氨基苯培养的巴贝斯虫基因表达。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2024-12-30 DOI: 10.1292/jvms.24-0395

Nami Matsuda, Minori Ito, Yuka Nukada, Miyuki Toyoma, Kazuya Nagai, Tomoki Motegi, Tomoya Morita, Masahiro Yamasaki

{"title":"Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing.","authors":"Nami Matsuda, Minori Ito, Yuka Nukada, Miyuki Toyoma, Kazuya Nagai, Tomoki Motegi, Tomoya Morita, Masahiro Yamasaki","doi":"10.1292/jvms.24-0395","DOIUrl":"10.1292/jvms.24-0395","url":null,"abstract":"A comprehensive and quantitative method to compare gene expression may be useful for investigating the mechanisms responsible for diminazene aceturate (DA) resistance in Babesia gibsoni. Therefore, the gene expression of B. gibsoni cultured with DA was compared with those without DA using RNA sequencing (RNA-seq). Total RNA extracted from the parasites cultured with or without DA was examined using two next-generation sequencers, the 454 GS Junior and MiniSeq systems. We aimed to detect the genes differentially expressed between parasites cultured with and without DA by mapping the reads against de novo assembled contigs. The contigs, the amounts of which were more than five-fold higher in the parasite with DA than that without DA, were searched using BLAST®, and two contigs were found as parasite genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) indicated that the expression levels of both genes were significantly higher in the parasites cultured with DA than those without DA. The nucleotide sequences of two contigs established using RNA-seq were similar to those found using direct sequencing, although the 5'- and 3'-end of those sequences were different between the two sequencing methods. In conclusion, we successfully utilized RNA-seq analysis to compare gene expression between parasites cultured with and without DA. RNA-seq can be used for comprehensive and quantitative analyses of gene expression in Babesia parasites.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"181-188"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipoprotein composition of calves before and after weaning and comparison with adult cows. 犊牛断奶前后的脂蛋白组成以及与成年母牛的比较。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2024-12-16 DOI: 10.1292/jvms.24-0172

Hitomi Sato, Ren Kumano, Rika Fukumori, Shin Oikawa

{"title":"Lipoprotein composition of calves before and after weaning and comparison with adult cows.","authors":"Hitomi Sato, Ren Kumano, Rika Fukumori, Shin Oikawa","doi":"10.1292/jvms.24-0172","DOIUrl":"10.1292/jvms.24-0172","url":null,"abstract":"Calves experience a significant decrease in dietary lipid intake with weaning. However, changes in lipoprotein metabolism during this period has not been described. Therefore, to evaluate changes in lipoprotein metabolism by weaning and age, differences in lipoprotein lipid concentrations and proportions were compared before and after weaning and between calves and adult cows. Blood samples were collected from 27 Holstein calves before (4 weeks old) and after weaning (13 weeks old). Blood samples were also collected from 10 adult cows (5 dry and 5 lactating cows). As the results, total lipid concentrations (triglycerides + total cholesterol + phospholipids) in low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were higher for pre-weaning calves than those in post-weaning calves. However, there were no differences in lipid proportions contained in each fraction. Lipid concentrations were higher in adult cows than in calves for all fractions: very low-density lipoprotein (VLDL), LDL and HDL. The proportion of total cholesterol and phospholipids in the LDL fraction was higher in calves compared to adult cows and the proportion of lipids in the HDL fraction was higher in adult cows compared to calves. These results showed that weaning caused litte changes in lipid concentrations in each lipoprotein fraction, but that adult cows showed higher lipid concentration in VLDL, LDL, and HDL. In addition, more lipids were contained in HDL for adult cows than in calves.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"167-170"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the effectiveness of four antihistamines with olopatadine in healthy Thoroughbred horses. 四种抗组胺药与奥洛他定对健康纯种马的疗效比较。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2025-01-14 DOI: 10.1292/jvms.24-0412

Yasuhiko Ikeda, Taisuke Kuroda, Hiroshi Mita, Norihisa Tamura, Minoru Ohta

引用次数: 0

An accelerometry-based, low cost and non-invasive respiration monitoring in anesthetized mice. 基于加速度计的麻醉小鼠低成本无创呼吸监测。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2024-12-31 DOI: 10.1292/jvms.24-0457

Natsuki Akashi, Yui Miyagawa, Hiroaki Kuno, Masumi Eto, Tetsuya Akagi

引用次数: 0

Correlation of forelimb long bone morphology with specific functional adaptations of locomotion. 前肢长骨形态与特定运动功能适应的相关性。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-04 Epub Date: 2024-12-26 DOI: 10.1292/jvms.24-0335

Ai Ito, Hideki Endo

{"title":"Correlation of forelimb long bone morphology with specific functional adaptations of locomotion.","authors":"Ai Ito, Hideki Endo","doi":"10.1292/jvms.24-0335","DOIUrl":"10.1292/jvms.24-0335","url":null,"abstract":"The significance of forelimb morphology has been discussed with a focus on specific morphological aspects; nonetheless, the correlation of overall morphology, including various linear measurements, with respect to ecological preference and adaptation has not been extensively explored, particularly using multiple taxa. We investigated the morphological characteristics of the long bones in the forelimbs of mammalian species and their relationship with specific functional adaptations using 20 linear measurements and 22 terrestrial species from seven orders. Principal component analysis and canonical discriminant analysis showed that the lengths of the humerus, radius, and ulna as well as the distance from the smallest width to the proximal and distal ends, and the deltoid tuberosity length distinguished four adaptations: arboreal, terrestrial, fossorial, and semi-aquatic. Moreover, our findings indicate that the distance from the smallest width to the proximal and distal ends, the deltoid tuberosity length, and the breadth of the distal end of the humerus are crucial in differentiating semi-arboreal, aquatic, and fossorial species.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"198-206"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0