Journal of Veterinary Medical Science最新文献_第2页

Campylobacter fetus isolates from both human patients and healthy cattle carry three distinct cytolethal distending toxin (cdt) gene clusters. 从人类患者和健康牛身上分离出的胎儿弯曲杆菌携带三种不同的细胞致死膨胀毒素（ctt）基因簇。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-28 DOI: 10.1292/jvms.24-0336

Wen Wen, Noritoshi Hatanaka, Srinuan Somroop, Sharda Prasad Awasthi, Atsushi Hinenoya, Shinji Yamasaki

{"title":"Campylobacter fetus isolates from both human patients and healthy cattle carry three distinct cytolethal distending toxin (cdt) gene clusters.","authors":"Wen Wen, Noritoshi Hatanaka, Srinuan Somroop, Sharda Prasad Awasthi, Atsushi Hinenoya, Shinji Yamasaki","doi":"10.1292/jvms.24-0336","DOIUrl":"https://doi.org/10.1292/jvms.24-0336","url":null,"abstract":"Campylobacter fetus is a zoonotic pathogen. Although the precise virulence mechanisms have not yet been fully elucidated, cytolethal distending toxin (CDT) is considered as one of the well-characterized virulence factors in Campylobacter. In silico analysis of the genome of C. fetus type strain of ATCC27374T indicates that there are three cdt gene clusters, Cfcdt-I, Cfcdt-II and Cfcdt-III. However, it is not clear whether these clusters are ubiquitously present in C. fetus and their association with diseases in humans and animals. In this study, we have analyzed the distribution and nucleotide sequences of these cdt gene clusters in 137 C. fetus strains isolated from human patients and healthy cattle. MLST and PFGE were also applied to determine clonal relationship between C. fetus strains isolated from patients and cattle. We found all C. fetus strains carry three Cfcdt gene clusters by colony hybridization assay and the strains belonged to 38 different pulsotypes. Whole genome sequencing of 38 C. fetus strains was carried out to determine the entire cdt gene cluster sequences and their sequence type (ST). Among 38 strains, six STs were identified, and each cdt gene cluster showed high similarity (>99%). Interestingly, some of these Cfcdt genes are more similar to the cdt genes of other Campylobacter species than other Cfcdt gene types. Altogether, the results suggest that three Cfcdt gene clusters are highly conserved in C. fetus and the strains belonging to ST-6 may be more pathogenic to human.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

End-tidal concentration of sevoflurane for optimal surgical conditions in pregnant sheep: a pragmatic approach to a retrospective observational study. 七氟醚潮气末浓度为妊娠羊提供最佳手术条件：一项回顾性观察研究的实用方法。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-25 DOI: 10.1292/jvms.24-0354

Tom Bleeser, Simen Vergote, David Basurto, Ignacio Valenzuela, Luc Joyeux, Francesca Russo, Yada Kunpalin, Doaa Emam, Marc VAN DE Velde, Sarah Devroe, Jan Deprest, Steffen Rex

引用次数: 0

Dogs with severe tracheal flattening exhibit lower degrees of left lateralization of the cervical esophagus. 气管严重扁平的狗狗颈部食管左侧化程度较低。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-25 DOI: 10.1292/jvms.24-0270

Kenji Teshima, Teppei Fujimoto, Naoko Shiozawa, Chieko Ishikawa, Yoshiki Yamaya

引用次数: 0

Establishment of new mouse model for allergic dermatitis showing severe fibrosis. 建立显示严重纤维化的过敏性皮炎新小鼠模型。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-24 DOI: 10.1292/jvms.24-0394

Yusuke Yamada, Kyoko Yoshizaki, Masashi Sakurai, Masahiro Morimoto

{"title":"Establishment of new mouse model for allergic dermatitis showing severe fibrosis.","authors":"Yusuke Yamada, Kyoko Yoshizaki, Masashi Sakurai, Masahiro Morimoto","doi":"10.1292/jvms.24-0394","DOIUrl":"https://doi.org/10.1292/jvms.24-0394","url":null,"abstract":"Allergic dermatitis (AD) is a skin disease characterized by a chronic inflammation caused by immune dysregulation. In the histopathology of patients with AD, there are several features, such as accumulation of eosinophils and mast cells, hyperkeratosis, and dermal fibrosis which are related to the exacerbation of AD. Mast cells and eosinophils are thought to be involved in fibrosis, but the details are unknown. Yama mouse is an inbred mouse showing genetically eosinophilia. If eosinophils have significant effect on fibrosis, it may be possible to establish a new AD model with severe fibrosis. In this study, AD was induced by applying dinitrofluorobenzene to mice auricle. Yama mice showed AD lesion with more severe dermal fibrosis with severe eosinophil infiltration than Balb/c and Nc/nga mice. The expression of transforming growth factor-β (TGF-β), a cytokine important for fibrosis, was not significantly different among Yama, Balb/c, and Nc/nga mice, while the expression of interleukin-4 (IL-4), which is also mediator of tissue fibrosis, was increased only in Yama mice. The results of this study showed that AD with more severe fibrosis could be induced in Yama mice than in Balb/c and Nc/nga mice. In Yama mice, it can be concluded that the severe fibrosis is TGF-β independent, and IL-4 would be the main mediator of severe fibrosis. This mouse model may be useful for elucidating the mechanism of fibrosis in chronic AD, and for conducting research leading to the development of new therapies.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nodal T-cell lymphoma with eosinophilic infiltration and sclerosing fibroplasia in a cat with eosinophilia. 一只患有嗜酸性粒细胞增多症的猫患上了伴有嗜酸性粒细胞浸润和硬化性纤维增生的结节性 T 细胞淋巴瘤。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-22 DOI: 10.1292/jvms.24-0225

Tamami Suzuki, Jumpei Yamazaki, Kouta Yamaguchi, Keisuke Aoshima, Takashi Kimura

引用次数: 0

Salinomycin induces apoptosis and potentiates the antitumor effect of doxorubicin against feline mammary tumor 2.5D organoids. 盐霉素诱导细胞凋亡并增强多柔比星对猫乳腺肿瘤2.5D器官组织的抗肿瘤作用。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-22 DOI: 10.1292/jvms.24-0344

Yishan Liu, Mohamed Elbadawy, Haru Yamamoto, Amira Augomaa, Yusuke Ishihara, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki

{"title":"Salinomycin induces apoptosis and potentiates the antitumor effect of doxorubicin against feline mammary tumor 2.5D organoids.","authors":"Yishan Liu, Mohamed Elbadawy, Haru Yamamoto, Amira Augomaa, Yusuke Ishihara, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki","doi":"10.1292/jvms.24-0344","DOIUrl":"https://doi.org/10.1292/jvms.24-0344","url":null,"abstract":"Feline mammary tumors (FMT) are the third most common form of neoplasm in cats. The prognosis of FMT is poor due to its high malignancy and metastatic potential. The outcomes of treatment using the common anticancer drug doxorubicin (DOX) are unsatisfactory, with resistance inevitably leading to treatment failure and disease recurrence. Salinomycin (SAL), an antibiotic, has been reported to exert anticancer effects on both human and canine mammary tumors. To recapitulate the genetic and molecular imprints of the original tumor sample, we generated four strains of patient-derived FMT 2.5D organoids (FMTO) to examine the anti-tumor potential of SAL. Our results revealed that SAL decreased cell viability in a dose-dependent manner. Treatment of FMTO with SAL-induced cell apoptosis, represented by an upregulation of P21, Caspase-8, and Caspase-9, and increased activity of Caspase-3/7. The combination of low-dose SAL with DOX (SD) potentiated the cytotoxicity of the latter in both DOX-resistant and DOX-sensitive strains, promoting cell apoptosis and cell-cycle arrest. In vivo, experiments using FMTO-derived xenografts engrafted into mice revealed decreased tumor growth following SAL administration. In conclusion, SAL showed anticancer activity against FMTO and potentiated the anticancer effect of DOX by inhibiting cell proliferation and inducing apoptosis and cell cycle arrest. These results suggest that SAL may represent a new adjuvant treatment option for patients with FMT.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Situational syncope caused by vomiting in a cat. 猫咪呕吐引起的情况性晕厥。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-17 DOI: 10.1292/jvms.24-0058

Daiki Hirao, Ryohei Kato, Keisuke Sakaguchi, Takahiro Ohmori, Shohei Morita, Hiroshi Hasegawa, Aritada Yoshimura, Ryuji Fukushima

引用次数: 0

Identification of signaling pathways induced by porcine parvovirus in porcine kidney-15 cells based on weighted correlation network analysis. 基于加权相关网络分析鉴定猪副猪嗜血杆菌在猪肾-15细胞中诱导的信号通路

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-16 DOI: 10.1292/jvms.24-0082

Tingting Lu, Xia Ma, Xinghui Song, Li Zhao

{"title":"Identification of signaling pathways induced by porcine parvovirus in porcine kidney-15 cells based on weighted correlation network analysis.","authors":"Tingting Lu, Xia Ma, Xinghui Song, Li Zhao","doi":"10.1292/jvms.24-0082","DOIUrl":"https://doi.org/10.1292/jvms.24-0082","url":null,"abstract":"Porcine parvovirus (PPV) is an important trigger of reproductive issues in pigs. Infection of the porcine kidney-15 (PK-15) cells with PPV induces cell death and inflammation. To explore the impact of PPV infection on gene expression in PK-15 cells and to identify the associated signaling pathways, we performed weighted correlation network analysis (WGCNA) on both PPV-infected and uninfected cells. We identified the blue and brown modules, with the blue module demonstrating decreased gene expression and the brown module showing increased gene expression at 48 hr post-PPV induction. Gene Ontology (GO) analysis revealed that genes in the blue module were predominantly associated with cellular components, while those in the brown module were enriched in biological processes, including the immunological response to PPV infection. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the modules indicated that the \"Cytokine-cytokine receptor interaction\" was linked to PPV. Furthermore, we screened 124 target genes involved in more than 100 pathways that interact with C-X-C motif chemokine ligand 8 (CXCL8), which plays an essential role in regulating numerous biological processes. The signaling pathways we identified facilitate a comprehensive examination of the mechanisms of interactions initiated by PPV infection.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142479250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance of Salmonella isolates from cattle and horses with salmonellosis in Hokkaido, Japan. 日本北海道牛马沙门氏菌病沙门氏菌分离物的抗菌药耐药性。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-16 DOI: 10.1292/jvms.24-0236

Yoshimasa Sasaki, Shotaro Suzuki, Nobuyuki Kusaba, Nahid Rahman, Chihiro Aikawa, Masashi Okamura

引用次数: 0

Elephant endotheliotropic herpesvirus gB-specific antibody levels in sera of Asian elephants (Elephas maximus) in Japanese zoos. 日本动物园亚洲象（Elephas maximus）血清中大象内皮细胞疱疹病毒 gB 特异性抗体水平。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-10-16 DOI: 10.1292/jvms.23-0503

Kazuya Takehana, Tabitha E Hoornweg, Willem Schaftenaar, Victor Pgm Rutten, Cornelis Am de Haan, Keita Matsuno

引用次数: 0