Journal of Veterinary Medical Science最新文献_第2页

Bilateral congenital cystic eye with intraocular tissue differentiation in a horse. 一匹马的双侧先天性囊性眼伴有眼内组织分化。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-01 Epub Date: 2024-11-19 DOI: 10.1292/jvms.24-0285

Yuto Sano, Chisa Miura, Yuki Kinoshita, Miyu Kakimoto, Tomonori Tsuda, Kazuya Matsuda

引用次数: 0

Molecular detection of African swine fever virus in pork and pork products and associated risk factors in the Philippines. 菲律宾猪肉和猪肉产品中非洲猪瘟病毒的分子检测及相关风险因素。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-01 Epub Date: 2024-11-18 DOI: 10.1292/jvms.24-0193

Aaron Paul R Serdeña, John Michael G Bernardo, Gladys Maria V Pangga, Saubel Ezrael A Salamat, Trisha Nicole Agulto, Mark Joseph M Desamero, Cj Philip G Atienza, Gabriel John A Calumpang, Ray Miguel P Canlas, Maureen Shane M Castillo, Allou Gabriela M Danao, Rozella Marie M Espino, Aliza Marie A Jacinto, Laika Ann Dennise G Morales, John Norman Daniel B Rico, Cherry P Fernandez-Colorado

{"title":"Molecular detection of African swine fever virus in pork and pork products and associated risk factors in the Philippines.","authors":"Aaron Paul R Serdeña, John Michael G Bernardo, Gladys Maria V Pangga, Saubel Ezrael A Salamat, Trisha Nicole Agulto, Mark Joseph M Desamero, Cj Philip G Atienza, Gabriel John A Calumpang, Ray Miguel P Canlas, Maureen Shane M Castillo, Allou Gabriela M Danao, Rozella Marie M Espino, Aliza Marie A Jacinto, Laika Ann Dennise G Morales, John Norman Daniel B Rico, Cherry P Fernandez-Colorado","doi":"10.1292/jvms.24-0193","DOIUrl":"10.1292/jvms.24-0193","url":null,"abstract":"African swine fever (ASF) is a contagious hemorrhagic disease affecting both domestic and wild pigs with high mortality rate and severe socio-economic losses. Due to the virus' potential ability to remain infectious in suitable conditions and environments, it is imperative to identify risk factors that may contribute to its transmission. The Philippines' current ASF surveillance relies on pig blood samples. With this very limited epidemiological data, it is imperative to include raw meat and processed pork products due to high ASF transmission risks. In this study, a total of 384 raw pork and 384 processed pork products from selected wet markets were collected and detected the ASF virus (ASFv) VP72 gene using real-time polymerase chain reaction (rt-PCR), and the overall positivity rates were 10.16% and 10.68%, respectively. Moreover, an interview with wet market vendors was conducted, and the Least Absolute Shrinkage and Selection Operator (LASSO) regression was employed to identify possible factors associated with sample positivity. Results showed that ASFv-positive samples were linked to zoning status, rainy seasons, Longganisa preparation, selling different meat types, longer pork batches, and cleaning or disinfection in market stalls. Overall, ASFv contamination in pork and processed pork products can pose a threat to the swine industry, and market practices may further lead to ASFv persistence in these commodities which may contribute to ASF spread. Therefore, there's a need to address this risk by strict surveillance and proper handling procedures throughout the food supply chain.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"13-27"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation state of Akt in the heart during artificial deep hypothermia in Syrian hamsters.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-31 DOI: 10.1292/jvms.24-0369

Yuuki Horii, Manami Kurata, Yuma Yoshida, Hiroki Sakai, Takahiko Shiina, Yasutake Shimizu

{"title":"Phosphorylation state of Akt in the heart during artificial deep hypothermia in Syrian hamsters.","authors":"Yuuki Horii, Manami Kurata, Yuma Yoshida, Hiroki Sakai, Takahiko Shiina, Yasutake Shimizu","doi":"10.1292/jvms.24-0369","DOIUrl":"https://doi.org/10.1292/jvms.24-0369","url":null,"abstract":"Hibernating animals show a remarkable decrease in body temperature without accompanying serious organ damage. Active hypometabolism may be involved in the protective mechanisms. Therefore, in the present study, the phosphorylation status of Akt was used to examine whether metabolism is actively reduced during artificial hypothermia in hamsters. Hypothermia induced by activation of the central adenosine A1 receptor (A1AR) resulted in dephosphorylation of Akt in the heart, as in that of hibernating hamsters. Since phosphorylated Akt almost totally disappeared, it is probable that cellular metabolism is actively reduced in A1AR-mediated hypothermia. Surprisingly, phosphorylation of Akt was significantly, but not totally, reduced even when hypothermia was induced by a combination of inhalation anesthesia and cooling. It can thus be considered that active regulation for the reduction of metabolic activity occurs in A1AR-mediated hypothermia as well as in anesthesia-induced hypothermia, though to a lesser extent. In both hypothermic conditions, serious arrhythmias were rarely observed. In anesthesia-induced hypothermia, during which dephosphorylation of Akt occurred only partially, there was no obvious organ damage in histopathological examination. Taken together, our findings suggest that artificial hypothermia causes active hypometabolism and has therapeutically applicable properties.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An accelerometry-based, low cost and non-invasive respiration monitoring in anesthetized mice.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-31 DOI: 10.1292/jvms.24-0457

Natsuki Akashi, Yui Miyagawa, Hiroaki Kuno, Masumi Eto, Tetsuya Akagi

引用次数: 0

Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-30 DOI: 10.1292/jvms.24-0395

Nami Matsuda, Minori Ito, Yuka Nukada, Miyuki Toyoma, Kazuya Nagai, Tomoki Motegi, Tomoya Morita, Masahiro Yamasaki

{"title":"Analysis of gene expression of Babesia gibsoni cultured with diminazene aceturate using RNA sequencing.","authors":"Nami Matsuda, Minori Ito, Yuka Nukada, Miyuki Toyoma, Kazuya Nagai, Tomoki Motegi, Tomoya Morita, Masahiro Yamasaki","doi":"10.1292/jvms.24-0395","DOIUrl":"https://doi.org/10.1292/jvms.24-0395","url":null,"abstract":"A comprehensive and quantitative method to compare gene expression may be useful for investigating the mechanisms responsible for diminazene aceturate (DA) resistance in Babesia gibsoni. Therefore, the gene expression of B. gibsoni cultured with DA was compared with those without DA using RNA sequencing (RNA-seq). Total RNA extracted from the parasites cultured with or without DA was examined using two next-generation sequencers, the 454 GS Junior and MiniSeq systems. We aimed to detect the genes differentially expressed between parasites cultured with and without DA by mapping the reads against de novo assembled contigs. The contigs, the amounts of which were more than five-fold higher in the parasite with DA than that without DA, were searched using BLAST®, and two contigs were found as parasite genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) indicated that the expression levels of both genes were significantly higher in the parasites cultured with DA than those without DA. The nucleotide sequences of two contigs established using RNA-seq were similar to those found using direct sequencing, although the 5'- and 3'-end of those sequences were different between the two sequencing methods. In conclusion, we successfully utilized RNA-seq analysis to compare gene expression between parasites cultured with and without DA. RNA-seq can be used for comprehensive and quantitative analyses of gene expression in Babesia parasites.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of forelimb long bone morphology with specific functional adaptations of locomotion.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-26 DOI: 10.1292/jvms.24-0335

Ai Ito, Hideki Endo

引用次数: 0

Influences of the short-term dietary carbohydrate restriction on the chicken cecal tonsil.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-25 DOI: 10.1292/jvms.24-0409

Md Salahuddin, Md Al-Amin, Kohzy Hiramatsu, Kazumi Kita

{"title":"Influences of the short-term dietary carbohydrate restriction on the chicken cecal tonsil.","authors":"Md Salahuddin, Md Al-Amin, Kohzy Hiramatsu, Kazumi Kita","doi":"10.1292/jvms.24-0409","DOIUrl":"https://doi.org/10.1292/jvms.24-0409","url":null,"abstract":"This study aims to clarify influences of the short-term carbohydrate (CHO) restriction on plasma cells in chicken cecal tonsil (CT) using immunohistochemical and morphometrical techniques. Healthy male layer chickens were randomly divided into the control and three experimental groups. The three experimental groups were fed with diets containing 50%, 25%, and 0% CHO of that in the control diet, respectively. Following the experimental period for 7 days, CTs were collected from each chicken. Immunohistochemistry was performed to detect immunoglobulin (Ig) A, G and M. The density of IgA-immunoreactive cells was significantly lower in the lymphoid follicles (LFs) and diffuse lymphoid tissues (DLTs) in CTs of the 0% group compared to those of the other groups. There was a positive correlation between daily CHO intake and the density of IgA-immunoreactive cells in the DLTs and LFs. The density of IgM-immunoreactive cells was significantly decreased as daily CHO intake decreased, and there was a positive correlation between these two factors in both DLTs and LFs. The density of IgG-immunoreactive cells was significantly lower in DLTs of the 0% group than those in the control and 50% groups. The density of IgG-immunoreactive cells was significantly decreased with a decrease in daily CHO intake in DLT but not in LF. These findings demonstrated that dietary CHO had a significant effect on the density of plasma cells in the CTs exhibiting a declining trend with a decrease of ingested CHO volume.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological identification and first molecular characterization of Lobothorax typus (Isopoda: Cymothoidae) parasitizing Trichiurus lepturus (Scombriformes: Trichiuridae) in the Philippines.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-18 DOI: 10.1292/jvms.24-0388

Adrian Miki C Macalanda, Eloiza May S Galon, John Carlo T Hipolito, Nikki S Simo, Gideon F Gayares, Rezin C Bahia, Vernadyn A Morillo, Emmanuel R Mago, Cherry R Alvarez

引用次数: 0

Lipoprotein composition of calves before and after weaning and comparison with adult cows. 犊牛断奶前后的脂蛋白组成以及与成年母牛的比较。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-16 DOI: 10.1292/jvms.24-0172

Hitomi Sato, Ren Kumano, Rika Fukumori, Shin Oikawa

{"title":"Lipoprotein composition of calves before and after weaning and comparison with adult cows.","authors":"Hitomi Sato, Ren Kumano, Rika Fukumori, Shin Oikawa","doi":"10.1292/jvms.24-0172","DOIUrl":"https://doi.org/10.1292/jvms.24-0172","url":null,"abstract":"Calves experience a significant decrease in dietary lipid intake with weaning. However, changes in lipoprotein metabolism during this period has not been described. Therefore, to evaluate changes in lipoprotein metabolism by weaning and age, differences in lipoprotein lipid concentrations and proportions were compared before and after weaning and between calves and adult cows. Blood samples were collected from 27 Holstein calves before (4 weeks old) and after weaning (13 weeks old). Blood samples were also collected from 10 adult cows (5 dry and 5 lactating cows). As the results, total lipid concentrations (triglycerides + total cholesterol + phospholipids) in low-density lipoprotein (LDL) and high-density lipoprotein (HDL) were higher for pre-weaning calves than those in post-weaning calves. However, there were no differences in lipid proportions contained in each fraction. Lipid concentrations were higher in adult cows than in calves for all fractions: very low-density lipoprotein (VLDL), LDL and HDL. The proportion of total cholesterol and phospholipids in the LDL fraction was higher in calves compared to adult cows and the proportion of lipids in the HDL fraction was higher in adult cows compared to calves. These results showed that weaning caused litte changes in lipid concentrations in each lipoprotein fraction, but that adult cows showed higher lipid concentration in VLDL, LDL, and HDL. In addition, more lipids were contained in HDL for adult cows than in calves.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arterial supply to the adrenal gland in the common marmoset (Callithrix jacchus). 普通狨猴（Callithrix jacchus）肾上腺的动脉供应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-16 DOI: 10.1292/jvms.24-0427

Tetsuhito Kigata, Keiko Moriya-Ito, Yoshiko Honda

{"title":"Arterial supply to the adrenal gland in the common marmoset (Callithrix jacchus).","authors":"Tetsuhito Kigata, Keiko Moriya-Ito, Yoshiko Honda","doi":"10.1292/jvms.24-0427","DOIUrl":"https://doi.org/10.1292/jvms.24-0427","url":null,"abstract":"The common marmoset (Callithrix jacchus) has attracted much attention as a nonhuman primate animal model for stress-related experiments. The adrenal gland plays a key role in stress responses and has abundant vasculature, through which the hypothalamic-pituitary axis hormonally controls its activity. Understanding the arterial supply pattern to the gland is fundamental to understanding its function and performing experiments. We therefore traced the arteries supplying the adrenal gland in six male and nine female common marmosets using the latex injection method. The adrenal artery originates from one to four parent arteries, including the caudal phrenic, celiac, left gastric, gonadal, and renal arteries and the abdominal aorta. The branching pattern of the adrenal arteries was divided into Types 1 to 4, having 1-4 parent arteries, respectively. Type 2 was most frequent on the right side (seven of 15 halves, 46%), whereas Type 3 was most frequent on the left (eight of 15 halves, 53%). The number of cranial, middle, and caudal adrenal arteries ranged from one to four in the cranial, one to five in the middle, and one to six in the caudal adrenal arteries. The total number of adrenal arteries varied from three to 13, predominantly as five on one side. The present study revealed frequent individual variations in the origin and number of adrenal arteries in the common marmoset, which may be informative for further research involving the adrenal glands in this species.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0