Journal of Veterinary Medical Science最新文献_第2页

Effect of lactoferrin on biofilm formation of bovine mastitis-causing Staphylococcus species. 乳铁蛋白对牛乳腺炎葡萄球菌生物膜形成的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-06 DOI: 10.1292/jvms.25-0230

Kumala Seraphina, Rin Furukido, Naoki Isobe, Takahiro Nii, Yuzo Kurokawa, Naoki Suzuki

{"title":"Effect of lactoferrin on biofilm formation of bovine mastitis-causing Staphylococcus species.","authors":"Kumala Seraphina, Rin Furukido, Naoki Isobe, Takahiro Nii, Yuzo Kurokawa, Naoki Suzuki","doi":"10.1292/jvms.25-0230","DOIUrl":"https://doi.org/10.1292/jvms.25-0230","url":null,"abstract":"The most common bovine mastitis pathogen is the Staphylococcus species, consisting of Staphylococcus aureus and non-aureus staphylococci (NAS). Lactoferrin (Lf) is an iron-binding protein with antimicrobial and antibiofilm properties. Lf has a metal-free form (apo-Lf) and a natural form (native-Lf), and their differences were reported to affect their activity against bacteria. However, its effects on bovine mastitis-causing Staphylococcus spp. remain unclear. Fifteen S. aureus and 49 NAS strains were isolated from bovine mastitis cases, and their growth and biofilm-forming abilities were observed. Bacterial growth and biofilm formation were observed by culturing each strain with/without bovine milk apo-/native-lactoferrin (200 μg/mL). Without Lf treatment, the growth and biofilm formation abilities of S. aureus were significantly higher and lower, respectively, than those of NAS. The growth of S. aureus and NAS significantly decreased during apo-Lf treatment and significantly reduced the total amount of biofilm produced by S. aureus whereas native-LF treatment did not affect the growth and biofilm formation of Staphylococcus species. These results confirmed the ability of Lf to act as an antimicrobial and antibiofilm substance against mastitis-causing Staphylococcus spp., although various responses from each strain were observed. Additionally, the iron-binding state of Lf affected growth but did not affect the biofilm formation ability. Differences in the responses of Staphylococcus strains to Lf may help explain their pathogenicity, requiring further research.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal expression of diamine oxidase (DAO) and the relationship between blood DAO activity and haptoglobin concentration in diarrheal calves. 腹泻犊牛肠道二胺氧化酶（DAO）表达及血DAO活性与触珠蛋白浓度的关系

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-03 DOI: 10.1292/jvms.25-0324

Akihiro Kozutsumi, Minoru Okamoto, Hana Takeuchi, Marina Otsuka, Kenji Tsukano, Nao Kondo, Kana Kamitani, Keiko Ikeda, Natsuki Takami, Haruyuki Hirata, Kazuyuki Suzuki

{"title":"Intestinal expression of diamine oxidase (DAO) and the relationship between blood DAO activity and haptoglobin concentration in diarrheal calves.","authors":"Akihiro Kozutsumi, Minoru Okamoto, Hana Takeuchi, Marina Otsuka, Kenji Tsukano, Nao Kondo, Kana Kamitani, Keiko Ikeda, Natsuki Takami, Haruyuki Hirata, Kazuyuki Suzuki","doi":"10.1292/jvms.25-0324","DOIUrl":"https://doi.org/10.1292/jvms.25-0324","url":null,"abstract":"The objectives of this study were (1) to confirm the expression of diamine oxidase (DAO) in the intestines of healthy calves immune-histologically and to clarify whether the expression of DAO in the intestines of calves with diarrhea differs from that of healthy animals, and (2) to clarify the relationship between DAO and intestinal inflammation using haptoglobin (HPT), a biomarker commonly used to evaluate inflammation. The abomasum, duodenum, jejunum and ileum, cecum, colon, and rectum of each animal were sampled at necropsy for immunohistochemical staining targeting DAO. DAO was diffusely expressed in all areas from the abomasum to the rectum in healthy calves, whereas its expression was reduced in calves with diarrhea. Four Japanese black calves with mild dehydration caused by diarrhea were included in the study to investigate the potential relationship between DAO and HPT. DAO activity was extremely low in diarrhea cases, whereas HPT levels were high. When an oral rehydration solution (ORS) was administered to these cases for three consecutive days, HPT significantly decreased logarithmically and fecal properties returned to normal on the fourth day of treatment. In contrast, DAO activities were significantly increased by oral rehydration therapy (ORT), and DAO activities were significantly negatively correlated with HPT concentrations. The present results confirmed that DAO is expressed in the epithelial cells of the intestinal mucosa in cattle, and that both the small and large intestines significantly contribute to blood DAO levels. Furthermore, DAO expression was reduced by enteritis.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematological and blood biochemical parameters of Tokara horses in Kaimon Foothills Nature Park. 开曼山麓自然公园托卡拉马血液学和血液生化指标。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-03 DOI: 10.1292/jvms.25-0340

Noriyuki Yamashita, Takashi Hatazoe, Kazuhiro Misumi, Seiji Hobo

引用次数: 0

Impact of different culture media and drying treatment on the isolation of Melissococcus plutonius, the causative agent of European foulbrood, from honey bee larval specimens. 不同培养基和干燥处理对蜜蜂幼虫标本中欧洲臭蝇病原菌——深蜂美利索球菌分离的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-02 DOI: 10.1292/jvms.25-0347

Yuko Kitamura, Yusuke Kusajima, Yuka Sekiya, Reiko Kawaguchi, Yuka Hayashi, Hisako Kakinuma, Mariko Okamoto, Daisuke Takamatsu

{"title":"Impact of different culture media and drying treatment on the isolation of Melissococcus plutonius, the causative agent of European foulbrood, from honey bee larval specimens.","authors":"Yuko Kitamura, Yusuke Kusajima, Yuka Sekiya, Reiko Kawaguchi, Yuka Hayashi, Hisako Kakinuma, Mariko Okamoto, Daisuke Takamatsu","doi":"10.1292/jvms.25-0347","DOIUrl":"https://doi.org/10.1292/jvms.25-0347","url":null,"abstract":"Melissococcus plutonius, the bacterium causing European foulbrood in honey bees, includes two distinct types: typical strains, which are fastidious and not easy to culture, and atypical strains, which grow more robustly and tolerate higher sodium levels. Several culture media have been used to isolate this pathogen, and growth dynamics and survival vary according to strain type and medium. The most commonly used media differs across countries, as does the ratio of typical to atypical strains isolated, implying that the choice of medium influences the type of M. plutonius that is more likely to be recovered. This study examined how two commonly used media, Basal and KSBHI, affect the isolation of typical and atypical strains from larval samples co-infected with both types. Both media supported the growth of both strains, and no isolation preference was observed, although KSBHI tended to yield more non-M. plutonius bacteria. Applying a drying treatment to larval smears reduced these secondary invaders but it shifted the isolation balance toward atypical strains. Drying resistance tests revealed that atypical strains survived longer under dry conditions than typical strains, which likely explains their increased recovery after drying. These results indicate that while both media can isolate either strain type, using both media in parallel is better for ensuring isolation of both types. Additionally, although drying reduces secondary invaders, it may bias the results toward atypical strains. These insights contribute to a more accurate diagnosis and epidemiological understanding of European foulbrood.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first outbreak of equine coronavirus infection in 13 years among draft horses at Obihiro Racecourse in Japan in 2025. 2025年，日本大宽赛马场（Obihiro Racecourse）的马中爆发了13年来的首次马冠状病毒感染。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-08-04 DOI: 10.1292/jvms.25-0271

Natsuko Fukumoto, Hiroshi Bannai, Nanako Kawanishi, Makoto Shibata, Daiki Kishi, Yoshinori Kambayashi, Koji Tsujimura, Manabu Nemoto

{"title":"The first outbreak of equine coronavirus infection in 13 years among draft horses at Obihiro Racecourse in Japan in 2025.","authors":"Natsuko Fukumoto, Hiroshi Bannai, Nanako Kawanishi, Makoto Shibata, Daiki Kishi, Yoshinori Kambayashi, Koji Tsujimura, Manabu Nemoto","doi":"10.1292/jvms.25-0271","DOIUrl":"10.1292/jvms.25-0271","url":null,"abstract":"Equine coronavirus (ECoV) causes fever, anorexia and sometimes enteric signs (e.g. diarrhea) in adult horses. Three ECoV outbreaks among draft horses at the same Japanese racecourse have been reported previously, in 2004, 2009 and 2012. We describe the epidemiological, clinical and genetic features of the fourth ECoV outbreak at the same racecourse in 2025. In January 2025, a sudden increase in the number of draft horses showing various combinations of fever, anorexia and enteric signs was observed at Obihiro Racecourse, in Hokkaido, Japan. Of about 800 horses, 176 showed clinical signs. Specifically, 83.5% (147/176) horses had fever, 98.9% (174/176) were anorexic, and 10.2% (18/176) had enteric signs. Real-time reverse transcription -polymerase chain reaction detected ECoV RNA in 23 of 26 horses. Seroconversion to ECoV was confirmed in 25 of 26 horses by neutralization assay. The results indicate that all 26 horses tested were infected with ECoV. Molecular analyses showed that the spike and nucleocapsid genes were highly conserved compared to other ECoVs. However, phylogenetic analysis showed that these genes of the 2025 virus did not form a group with the strains detected in the previous three outbreaks. These results indicate that ECoV was the cause of the sudden increase in the number of draft horses with fever, anorexia and enteric signs. Although the origin of the 2025 virus is unknown, this outbreak was likely caused by a virus different from the one that caused the previous outbreaks.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1158-1163"},"PeriodicalIF":1.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose and insulin responses following streptozotocin administration for the induction of a type 1 diabetes mellitus model in Microminipig. 链脲佐菌素诱导微型猪1型糖尿病模型后葡萄糖和胰岛素的反应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-08-19 DOI: 10.1292/jvms.25-0145

Maria Izumi, Kazuki Hano, Taiki Kato, Akihiro Hirata, Paing Thaw, Azusa Seki, Miyako Tanaka, Kozue Ochi, Takayoshi Suganami, Hitoshi Kitagawa, Masaki Takasu

引用次数: 0

A simple, inexpensive, and rapid method for measuring calcium concentrations in colostrum to predict postpartum milk fever in cows using a commercially available water quality test device. 一种简单、廉价、快速的测定初乳钙浓度的方法，使用市售的水质测试装置预测奶牛产后乳热。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-09-02 DOI: 10.1292/jvms.25-0298

Nao Kondo, Masaaki Maeda, Ryo Murotsuki, Tatsuya Ryoha, Marina Otsuka, Kenji Tsukano, Hiroaki Kozutsumi, Keiko Ikeda, Kana Kamitani, Minoru Okamoto, Haruyuki Hirata, Kazuyuki Suzuki

{"title":"A simple, inexpensive, and rapid method for measuring calcium concentrations in colostrum to predict postpartum milk fever in cows using a commercially available water quality test device.","authors":"Nao Kondo, Masaaki Maeda, Ryo Murotsuki, Tatsuya Ryoha, Marina Otsuka, Kenji Tsukano, Hiroaki Kozutsumi, Keiko Ikeda, Kana Kamitani, Minoru Okamoto, Haruyuki Hirata, Kazuyuki Suzuki","doi":"10.1292/jvms.25-0298","DOIUrl":"10.1292/jvms.25-0298","url":null,"abstract":"To diagnose milk fever (MF) in dairy cows under clinical practice, measurement of ionized calcium concentrations in blood using point-of-care (POC) devices is required. However, blood sampling poses a significant financial burden on farmers and increases the workload of veterinarians who must visit farms to perform the examinations. The present study aimed to evaluate whether the onset of MF could be predicted using colostrum samples, which can be collected by farmers themselves. Total calcium (tCa) concentrations in colostrum were measured using a commercially available water quality test device. The results showed that cows treated for MF had significantly higher tCa concentrations in colostrum (2,141.1 ± 623.7 mg/L) compared to cows without MF (1,546.3 ± 494.4 mg/L) (P=0.006). Receiver operating characteristic (ROC) curve analysis suggested that measuring tCa concentration in colostrum could be used to predict the onset of MF. When the cut-off value was set at >2,000 mg/L, the sensitivity and specificity were 66.7% and 88.9%, respectively. Results from these findings, measuring tCa concentration in colostrum using a water quality test device offers a simple, inexpensive, and rapid method for predicting the onset of MF in dairy cows.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1186-1193"},"PeriodicalIF":1.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144976694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced antioxidant and anti-Mycoplasma hyopneumoniae activities of combined Piper sarmentosum and guava leaf extracts. 胡椒叶和番石榴叶联合提取物增强抗氧化和抗肺炎支原体活性。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-08-19 DOI: 10.1292/jvms.25-0284

Luli Zhou, Jirui Wang, Maojun Liu, Guanyu Hou, Guang Rong, Renlong Lv, Dingfa Wang

{"title":"Enhanced antioxidant and anti-Mycoplasma hyopneumoniae activities of combined Piper sarmentosum and guava leaf extracts.","authors":"Luli Zhou, Jirui Wang, Maojun Liu, Guanyu Hou, Guang Rong, Renlong Lv, Dingfa Wang","doi":"10.1292/jvms.25-0284","DOIUrl":"10.1292/jvms.25-0284","url":null,"abstract":"Increasing antibiotic resistance in Mycoplasma hyopneumoniae (Mhp) necessitates alternative treatments. Piper sarmentosum extracts (PSE) and guava extracts (GE) show promise due to their antimicrobial and antioxidant properties. This study evaluates the combined in vitro effects of PSE and GE against Mhp and their antioxidant activities. Anti-Mhp activity was assessed using microdilution minimum inhibitory concentration (MIC) and synergy via fractional inhibitory concentration index (FICI), while antioxidant capacity was measured with 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays for individual extracts, and a 1:1 mixture (based on the results of FICI). Phytochemical profiling used high performance liquid chromatography (HPLC) to quantify main six bioactive compounds. HPLC showed PSE contained vitexin-2-O-rhamnoside (8.58 mg/g) and pellitorine (3.31 mg/g); GE had quercetin-3-O-glucuronide (6.85 mg/g), quercetin (3.72 mg/g), isoquercitrin (0.51 mg/g), and avicularin (1.37 mg/g). PSE (MIC 0.78 mg/mL) and GE (MIC 0.39 mg/mL) exhibited anti-Mhp effects, with their 1:1 combination being additive (FICI=0.75) and reducing MIC to 0.195 mg/mL. This mix also enhanced antioxidant activity by 9.0% in ABTS and 27.0% in FRAP assays. The 1:1 combination of PSE and GE demonstrated superior anti-Mhp activity (additive effect) and enhanced antioxidant properties (synergistic effect) compared to the individual extracts. These findings highlight the potential of these extracts as phytopharmaceutical agents.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1128-1135"},"PeriodicalIF":1.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144976697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examination of artificial oocyst excystation methods in the murine coccidium, Eimeria krijgsmanni. 克氏艾美耳球虫人工卵囊清除方法研究。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-09-03 DOI: 10.1292/jvms.25-0229

Toshiaki Tanaka, Mika Chatani, Asako Haraguchi, Makoto Matsubayashi, Hiromi Ikadai, Takane Kaneko, Tomohide Matsuo

{"title":"Examination of artificial oocyst excystation methods in the murine coccidium, Eimeria krijgsmanni.","authors":"Toshiaki Tanaka, Mika Chatani, Asako Haraguchi, Makoto Matsubayashi, Hiromi Ikadai, Takane Kaneko, Tomohide Matsuo","doi":"10.1292/jvms.25-0229","DOIUrl":"10.1292/jvms.25-0229","url":null,"abstract":"Eimeria spp. cause coccidiosis characterized by diarrhea and induce serious economic losses in livestock industries. Although several anti-coccidial drugs are currently available, the emergence of resistant strains and drug residues is problematic; therefore, the development of new drugs is needed. Since sporozoites of Eimeria spp. invade host intestinal epithelial cells and numerous merozoites are formed, drugs that target sporozoites are expected to be useful. We previously used murine Eimeria krijgsmanni as a model to examine anti-coccidial drug susceptibility; however, few studies have conducted drug evaluations against sporozoites. The establishment of excystation protocols is essential for progress in in vitro experiments using sporozoites because oocysts must be isolated from feces using complex techniques before the excystation process. Various artificial excystation protocols have been reported for each Eimeria spp.; however, those for E. krijgsmanni have not yet been examined. Therefore, 4 protocols described in previous studies were herein conducted for E. krijgsmanni. Pepsin was important for excystation in rodent Eimeria spp., and this was also the case for E. krijgsmanni. Excystation rates were higher with the physical disruption of oocyst walls than with pepsin. An incubation in HBSS containing 0.25% (w/v) trypsin and 0.1% (w/v) sodium taurocholate after a physical treatment achieved higher and the most stable excystation rates. Modifications to this method were also examined, and no improvements were observed. The optimal excystation protocol for E. krijgsmanni was elucidated as of now.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1204-1209"},"PeriodicalIF":1.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence and associated risk factors of epizootic hemorrhagic disease virus in cattle from the northern region of Peru: first serological report. 秘鲁北部地区牛流行性出血病病毒的血清流行率和相关危险因素：第一份血清学报告。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-10-01 Epub Date: 2025-08-29 DOI: 10.1292/jvms.25-0183

Kevin Ponce, Jessica Jurado, Mercy Ramirez, Luis Vargas-Rocha, Dennis A Navarro-Mamani

{"title":"Seroprevalence and associated risk factors of epizootic hemorrhagic disease virus in cattle from the northern region of Peru: first serological report.","authors":"Kevin Ponce, Jessica Jurado, Mercy Ramirez, Luis Vargas-Rocha, Dennis A Navarro-Mamani","doi":"10.1292/jvms.25-0183","DOIUrl":"10.1292/jvms.25-0183","url":null,"abstract":"Epizootic hemorrhagic disease virus (EHDV) is a vector-borne pathogen that affects both wild and domestic ruminants. Climate influences vector-borne diseases by driving vector migration to new areas, where they spread the virus. However, the lack of surveillance in some areas hinders accurate assessment the true disease burden. This study aimed to determine the seroprevalence of EHDV and associated risk factors in cattle from the northern region of Peru in 2022. Blood serum samples were collected from 578 cattle in the departments of Tumbes, Piura, Lambayeque, Cajamarca, and La Libertad and analyzed using cELISA. The overall EHDV seroprevalence was 17.82% (95% confidence interval 14.78-21.19). The highest proportion of seropositive animals was observed in two deparments closest to the equator, Piura and Tumbes (50%), followed by Lambayeque (39.1%) and Cajamarca (11.08%). In addition, the highest seroprevalence was found at temperatures ≤20°C (50.46%), wind speeds ≤3 m/sec (31.43%), and altitudes ≤1,260 masl (37.28%), with a significant decrease at higher elevations (P<0.05). After adjusting for temperature, the odds of EHDV seropositivity were significantly lower in cattle from areas located at >2,000 to ≤3,290 masl (Odds ratio [OR]=0.15) and >3,290 (OR=0.07), compared to those from the reference altitude category of ≤1,260 masl. Similarly, after adjusting for altitude, cattle from deparments with temperatures >20°C had significantly lower odds of seropositivity (OR=0.17) compared to those exposed to temperatures ≤20°C. This study reports anti-EHDV antibodies in cattle from northern Peru for the first time, highlighting associations with bioclimatic factors.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1180-1185"},"PeriodicalIF":1.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12508599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144976729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0