{"title":"Acanthomatous ameloblastoma with mucinous glandular differentiation in a dog.","authors":"Takayuki Mineshige, Satoshi Takagi, Takanori Shiga, Naoyuki Aihara, Hiromitsu Yoshida, Eiichi Kanai, Junichi Kamiie","doi":"10.1292/jvms.24-0374","DOIUrl":"10.1292/jvms.24-0374","url":null,"abstract":"<p><p>This report describes an atypical case of canine acanthomatous ameloblastoma (CAA) with mucinous glandular differentiation in a 12-year-old male Chihuahua with a right mandibular mass. Initially diagnosed as CAA by biopsy, computed tomography revealed bone lysis, prompting surgical excision. Histological examination showed lobular growth with two distinct neoplastic cell populations: typical CAA-like cells and a minor glandular structure population. Mucinous glandular differentiation in CAA is undocumented in veterinary literature. Immunohistochemistry showed positivity for cytokeratin AE1/AE3 in all tumor cells, and cytokeratin CAM5.2 and Alcian blue staining confirmed glandular cells and mucin. A low mitotic and K-i67 proliferation index suggested low-grade malignancy. This rare case highlights the need for comprehensive histopathological and immunohistochemical analysis to distinguish CAA from other oral tumors.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"194-197"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adrian Miki C Macalanda, Eloiza May S Galon, John Carlo T Hipolito, Nikki S Simo, Gideon F Gayares, Rezin C Bahia, Vernadyn A Morillo, Emmanuel R Mago, Cherry R Alvarez
{"title":"Morphological identification and first molecular characterization of Lobothorax typus (Isopoda: Cymothoidae) parasitizing Trichiurus lepturus (Scombriformes: Trichiuridae) in the Philippines.","authors":"Adrian Miki C Macalanda, Eloiza May S Galon, John Carlo T Hipolito, Nikki S Simo, Gideon F Gayares, Rezin C Bahia, Vernadyn A Morillo, Emmanuel R Mago, Cherry R Alvarez","doi":"10.1292/jvms.24-0388","DOIUrl":"10.1292/jvms.24-0388","url":null,"abstract":"<p><p>Parasitic diseases pose a threat to fisheries, negatively affecting the livelihood and food security of billions of people worldwide. Among these, parasitic isopods of fish are not well documented and studied. Thus, we examined the buccal cavity of 385 randomly selected saltwater fishes sold in a fish market in Naic, Cavite, Philippines. Here, 7/385 (1.82%) saltwater fishes, exclusively Trichiurus lepturus, harbored a parasitic isopod. Morphological observation and sequencing analysis of cytochrome c oxidase I (COI) and 16S rRNA genes confirmed the identity as Lobothorax typus. Phylogenetic tree based on these sequences showed that it is closely related to isolates from India and Japan. As such, further studies on the risk factors associated with L. typus infestation are urgently needed.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"189-193"},"PeriodicalIF":1.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beatriz Regina Rodrigues Carvalho, Ronalda Silva de Araujo, Danilo Kluyber, Arnaud Léonard Jean Desbiez, Mayara Grego Caiaffa, Mario Henrique Alves, Grazielle Soresini, Bruno de Lima Araújo, Maria Anete Lallo
{"title":"Encephalitozoon intestinalis infection in free-ranging giant anteater (Myrmecophaga ptridactyla) and armadillo species (Priodontes maximus, Euphractus sexcinctus, Cabassous squamicaudis).","authors":"Beatriz Regina Rodrigues Carvalho, Ronalda Silva de Araujo, Danilo Kluyber, Arnaud Léonard Jean Desbiez, Mayara Grego Caiaffa, Mario Henrique Alves, Grazielle Soresini, Bruno de Lima Araújo, Maria Anete Lallo","doi":"10.1292/jvms.24-0348","DOIUrl":"https://doi.org/10.1292/jvms.24-0348","url":null,"abstract":"<p><p>Microsporidia are emerging, opportunistic fungi that infect a diverse population of vertebrates and invertebrates. Mammals of the superorder Xenarthra can harbor and transmit several pathogens, acting as important sources of infection for spreading various zoonoses. Microsporidia have not yet been described in this group of animals, the aim of this study was to analyze the occurrence of the zoonotic microsporidian Encephalitozoon intestinalis, Encephalitozooncuniculi and Enterocytozoon bieneusi in the feces of giant anteater (Myrmecophaga tridactyla) and armadillo species (Priodontes maximus, Euphractus sexcinctus, Dasypus novemcinctus, Cabassous squamicaudis) monitored by Wild Animal Conservation Institute (ICAS) in Brazil. Fecal samples (n=127) were subjected to DNA extraction with the QIAamp Fast DNA Stool Mini Kit. Amplification by PCR was performed with generic primers and the product generated from this reaction was subjected to nested PCR with specific primers. Eleven samples tested positive for E. intestinalis, two from M. tridactyla (2/56, 3.6%), seven from E. sexcinctus (7/51, 13.7%), two from P. maximus (2/11, 18%) and one from C. squamicaudis (1/3, 33.3%). There was a predominance of positive results in adult animals, of both sexes across and in the Pantanal and Cerrado biomes. In conclusion, the prevalence in Xenarthra was 9.4%, with a higher occurrence in armadillos than in anteaters. Therefore, the species of wild mammals studied here should be considered reservoirs of microsporidian pathogens and have a relevant role in the concept of One Health.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aya Masuda, Natsumi Hayashi, Kaito Otsuru, Chinatsu Kobayashi, Sakura Miki, Fuko Takahata, Kanata Takahashi, Fumiaki Yamasaki, Jun Matsumoto
{"title":"Prevalence and patterns of ectoparasites infesting Pallas's squirrels (Callosciurus erythraeus) in Kanagawa Prefecture, Japan.","authors":"Aya Masuda, Natsumi Hayashi, Kaito Otsuru, Chinatsu Kobayashi, Sakura Miki, Fuko Takahata, Kanata Takahashi, Fumiaki Yamasaki, Jun Matsumoto","doi":"10.1292/jvms.24-0301","DOIUrl":"https://doi.org/10.1292/jvms.24-0301","url":null,"abstract":"<p><p>The Pallas's squirrel (Callosciurus erythraeus) has invaded fragmented woodlands in urban areas of Kanagawa Prefecture, where frequent human contact occurs. We examined 538 squirrels in Hayama-machi, Kanagawa Prefecture, for ectoparasite infestations. A total of 1164 lice, 877 fleas, and 231 ticks were retrieved from 297 (55.2%), 338 (62.8%), and 135 (25.1%) squirrels, respectively. The identified ectoparasite species were Neohaematopinus callosciuri, Ceratophyllus anisus, Haemaphysalis flava, Haemaphysalis hystricis, and Ixodes turdus. The prevalence of N. callosciuri and C.anisus was significantly higher in adult males (N. callosciuri 63.6%, C. anisus 70.4%) than that in adult females (N. callosciuri 36.1%, C. anisus 49.0%; P<0.0001). Such information on the dynamics of host-ectoparasite relations is crucial for evaluating the risk to public health.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shoichi Wakitani, Risako Kawabata, Sora Shirataki, Masahiro Yasuda
{"title":"Distribution of keratin subtypes in the thymus of Japanese black calves during acute thymic involution.","authors":"Shoichi Wakitani, Risako Kawabata, Sora Shirataki, Masahiro Yasuda","doi":"10.1292/jvms.24-0413","DOIUrl":"10.1292/jvms.24-0413","url":null,"abstract":"<p><p>Immunohistochemistry for keratins 5, 8, 14, and 18 was performed on Japanese Black calf thymuses at various stages of acute thymic involution. Keratins 5 and 14 were predominantly localized in the thymic medulla, while keratins 8 and 18 were broadly distributed throughout the parenchyma. Despite thymic involution, the distribution patterns of these keratins remained consistent. The cortical area, assessed by keratin 5 staining, progressively decreased with involution but retained approximately 40% of the total parenchyma even at the most severe stage. These results suggest that the thymic cortex shrinks but does not completely disappear during acute thymic involution in calves.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"131-134"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High-fat/high-sucrose diet-induced renal changes in obese diabetic mice: a comparison with db/db and KK-Ay mice.","authors":"Chika Oki, Kinuko Uno, Tomohiko Sasase, Takahiro Tsutsui, Keita Sekiguchi, Ayane Yamaguchi, Kouhei Mandai, Masami Shinohara, Miki Sugimoto, Tatsuya Maekawa, Katsuhiro Miyajima, Takeshi Ohta","doi":"10.1292/jvms.24-0313","DOIUrl":"10.1292/jvms.24-0313","url":null,"abstract":"<p><p>Many genetic and environmental factors are involved in the development and progression of diabetic kidney disease (DKD), and its pathology shows various characteristics. Animal models of DKD play an important role in elucidating its pathogenesis and developing new therapies. In this study, we investigated the pathophysiological features of two DKD animal models: db/db mice (background of hyperglycemia) and KK-Ay mice (background of hyperinsulinemia). Male and female mice were fed a high-fat/high-sucrose (HFS) diet for eight weeks. Two mouse models fed the HFS diet showed increases in urinary protein, kidney weight, and glomerular size, but these changes were pronounced in KK-Ay mice. Pathological examination revealed tubulointerstitial fibrosis in KK-Ay mice fed the HFS diet, but not in db/db mice. In addition, fat accumulation was observed in the macula densa of db/db mice and in the glomeruli of KK-Ay mice fed with the HFS diet. In conclusion, an HFS diet exacerbates renal lesions with tubulointerstitial fibrosis in KK-Ay mice, and KK-Ay mice fed an HFS diet are expected to be useful as a DKD model.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"138-146"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Honoka Nojo, Nobuo Murayama, Sanae A Ishijima, Rui Kano
{"title":"In vitro susceptibility testing of essential oils and their main components against antifungal-resistant Malassezia pachydermatis.","authors":"Honoka Nojo, Nobuo Murayama, Sanae A Ishijima, Rui Kano","doi":"10.1292/jvms.24-0342","DOIUrl":"10.1292/jvms.24-0342","url":null,"abstract":"<p><p>Canine Malassezia dermatitis (CMD) and otitis externa are generally treated by antifungal drugs. However, azole-resistant strains have been isolated from canine skin and ear canals worldwide. Phytochemicals isolated from essential oils are effective alternatives for inhibiting Malassezia pachydermatis. To evaluate the usefulness of phytochemicals against azole-resistant isolates, we performed in vitro susceptibility testing using the phytochemicals carvacrol, citral, and thymol. Eight antifungal-resistant isolates were obtained from 7 cases of dermatitis and 1 case of otitis externa during 2022 and 2023 from dogs in Tokyo and Kanagawa, Japan. Fungal susceptibility to carvacrol, citral, and thymol were assessed using the modified broth microdilution method. The minimum inhibitory concentrations (MICs) of the phytochemicals in all isolates were as follows: 0.03 to 0.125% for carvacrol; 0.03 to 0.125% for thymol; and 0.03% to 0.125% for citral. Based on these results, carvacrol, citral, and thymol appear to be effective against azole-resistant strains. The phytochemicals appear to be effective for treating antifungal-resistant cases of CMD and otitis externa.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"135-137"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Arterial supply to the adrenal gland in the common marmoset (Callithrix jacchus).","authors":"Tetsuhito Kigata, Keiko Moriya-Ito, Yoshiko Honda","doi":"10.1292/jvms.24-0427","DOIUrl":"10.1292/jvms.24-0427","url":null,"abstract":"<p><p>The common marmoset (Callithrix jacchus) has attracted much attention as a nonhuman primate animal model for stress-related experiments. The adrenal gland plays a key role in stress responses and has abundant vasculature, through which the hypothalamic-pituitary axis hormonally controls its activity. Understanding the arterial supply pattern to the gland is fundamental to understanding its function and performing experiments. We therefore traced the arteries supplying the adrenal gland in six male and nine female common marmosets using the latex injection method. The adrenal artery originates from one to four parent arteries, including the caudal phrenic, celiac, left gastric, gonadal, and renal arteries and the abdominal aorta. The branching pattern of the adrenal arteries was divided into Types 1 to 4, having 1-4 parent arteries, respectively. Type 2 was most frequent on the right side (seven of 15 halves, 46%), whereas Type 3 was most frequent on the left (eight of 15 halves, 53%). The number of cranial, middle, and caudal adrenal arteries ranged from one to four in the cranial, one to five in the middle, and one to six in the caudal adrenal arteries. The total number of adrenal arteries varied from three to 13, predominantly as five on one side. The present study revealed frequent individual variations in the origin and number of adrenal arteries in the common marmoset, which may be informative for further research involving the adrenal glands in this species.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"123-130"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Administration of 1-kestose to beluga reduces intestinal Turicibacter and collagenase gene levels, and blood creatinine levels.","authors":"Tadashi Fujii, Takashi Kamio, Yuichiro Akune, Hideaki Takahashi, Nobuhiro Kondo, Kohei Funasaka, Eizaburo Ohno, Yoshiki Hirooka, Takumi Tochio","doi":"10.1292/jvms.24-0131","DOIUrl":"10.1292/jvms.24-0131","url":null,"abstract":"<p><p>In recent years, an increased emphasis on enhancing the care and health management of captive marine mammals has been observed. Belugas (White Whale, Delphinapterus leucas), belonging to the family Monodontidae, are of considerable importance and often the centerpiece of aquarium collections worldwide. This study aimed to investigate the effects of the administration of prebiotics on the gut microbiota and overall health of the beluga. Prebiotic 1-kestose, a fructooligosaccharide comprising sucrose and fructose, was administered to three belugas, alongside their regular vitamin supplements for a duration of 8 weeks. 16S rRNA gene amplicon sequencing of intestinal DNA revealed that the relative abundance of the genus Turicibacter, a potentially pathogenic bacteria, significantly reduced after 1-kestose administration when compared to that at baseline (P=0.050). In addition, a quantitative PCR analysis revealed that the levels of collagenase gene, a putative virulence factor gene of Turicibacter, significantly reduced after 1-kestose administration (P=0.050). Blood creatinine levels that were initially above the normal value also reduced after 1-kestose administration (P=0.023). Therefore, this study demonstrated the potential of 1-kestose to improve the health and welfare of aquarium belugas.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"152-159"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Disseminated proliferative mesocestoidosis caused by Mesocestoides vogae (Cestoda: Cyclophyllidae) in a French bulldog in Japan.","authors":"Toshihiro Tokiwa, Hisashi Yoshimura, Mami Takase, Eigo Kato, Masateru Morisaki, Tatsushi Morita, Mitsuru Koutaka","doi":"10.1292/jvms.24-0411","DOIUrl":"10.1292/jvms.24-0411","url":null,"abstract":"<p><p>This report describes a case of disseminated proliferative mesocestoidosis in a French bulldog from Aichi, Japan. In July 2023, the dog presented with chronic enteritis, and histological examination of the endoscopic biopsy specimen revealed parasites with suckers. Three months later, numerous tetrathyridia were detected in the inguinal mass. In April 2024, polymorphic tetrathyridia were detected on the visceral organs. These parasites were identified as Mesocestoides vogae (Cyclophylidea: Mesocestoididae) based on morphological features and sequence analyses. In Japan, M. vogae has only been reported in domestic dogs, with five cases of intestinal infection and one case of abdominal infection. This report is the first case of both intestinal and extraintestinal infections in the westernmost region of Japan.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"147-151"},"PeriodicalIF":1.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}