Journal of Veterinary Medical Science最新文献_第4页

Distribution and Fasciola infection rates of Lymnaea snails and cattle in high-salinity areas of Mekong Delta, Vietnam.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-30 DOI: 10.1292/jvms.24-0528

Dang Thi Loan, Lam Thanh Nguyen, Tran Ngoc Bich, Nguyen Thuy Y Vi, Yasunobu Matsumoto

{"title":"Distribution and Fasciola infection rates of Lymnaea snails and cattle in high-salinity areas of Mekong Delta, Vietnam.","authors":"Dang Thi Loan, Lam Thanh Nguyen, Tran Ngoc Bich, Nguyen Thuy Y Vi, Yasunobu Matsumoto","doi":"10.1292/jvms.24-0528","DOIUrl":"https://doi.org/10.1292/jvms.24-0528","url":null,"abstract":"Fasciola-induced fascioliasis is a zoonotic disease with significant health and economic impacts on humans and livestock. Freshwater Lymnaea snails serve as intermediate hosts, contributing to the increasing prevalence of fascioliasis in cattle in coastal areas. The salinity tolerance of Lymnaea snails was investigated along with their distribution and Fasciola infection rates in both snails and grazing cattle in Ben Tre, Tra Vinh, and Soc Trang provinces in Mekong Delta, Vietnam, where seawater reversely enters into the paddy field during the dry season. Lymnaea snails were collected from 53 communes across the three provinces and analyzed for Fasciola larval infections. Additionally, cattle fecal samples were examined for the presence of Fasciola eggs. The salinity levels in the study areas ranged from 0.03% to 1.90%. In total, 1,152 Lymnaea snails including L. viridis and L. rubiginosa were collected. The maximum salinity levels of L. viridis and L. rubiginosa habitats were 0.64% and 0.74%, respectively. The Fasciola infection rates of L. viridis were 2.0% in low-salinity areas and 3.7% in high-salinity areas, whereas no L. rubiginosa infections were detected in either salinity area. The prevalence of cattle fascioliasis in high-salinity areas (35.7%) was similar to low-salinity areas (32.9%). This study is the first to demonstrate that L. viridis can thrive and sustain Fasciola transmission under high-salinity conditions, suggesting the salinity tolerance of these intermediate host snails as a key factor contributing to the expansion of fascioliasis in high-salinity coastal areas.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for the isolation of Escherichia albertii from diarrheal stool specimens.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-24 DOI: 10.1292/jvms.24-0500

Keiji Takehira, Sharda Prasad Awasthi, Noritoshi Hatanaka, Akira Nagita, Atsushi Hinenoya, Shinji Yamasaki

{"title":"Evaluation of a novel modified selective medium cefixime-tellurite-phosphate-xylose-rhamnose MacConkey agar for the isolation of Escherichia albertii from diarrheal stool specimens.","authors":"Keiji Takehira, Sharda Prasad Awasthi, Noritoshi Hatanaka, Akira Nagita, Atsushi Hinenoya, Shinji Yamasaki","doi":"10.1292/jvms.24-0500","DOIUrl":"https://doi.org/10.1292/jvms.24-0500","url":null,"abstract":"It is challenging to isolate Escherichia albertii from clinical specimens. Therefore, a medium that can selectively grow E. albertii and differentiate it from E. coli is earnestly desired. Here, we describe the evaluation of a recently developed selective differential medium, called cefixime-tellurite-phosphate-xylose-rhamnose-MacConkey (CT-PS-XR-MacConkey) medium, which enables the specific growth of E. albertii and differentiation of E. albertii (colorless) from E. coli (red) based on colony color and thus, facilitating the efficient isolation of E. albertii from diarrheal stool. When three E. albertii negative diarrheal stools were inoculated onto CT-PS-XR-MacConkey and xylose-rhamnose-melibiose (XRM) containing MacConkey agars, no colorless colonies were observed on both the media. However, when E. albertii was spiked into these three diarrheal stools, the ratio of colorless colonies to red colonies was higher on CT-PS-XR-MacConkey agar compared to XRM-MacConkey agar in all three samples. Notably, out of 105 Eacdt-gene PCR negative diarrheal stools 56 yielded colorless colonies on MacConkey agar while out of these 56 diarrheal stools, nine yielded colorless colonies on XRM-MacConkey but no colorless colonies were observed on CT-PS-XR-MacConkey agar. Furthermore, evaluation of these two media with five E. albertii positive-stool specimens revealed that the number of red colonies were constantly less, whereas that of colorless colonies were constantly more on CT-PS-XR-MacConkey agar, thus aiding in efficient isolation. Altogether, these results suggest that the CT-PS-XR-MacConkey agar could be a useful selective differential medium for isolation of E. albertii from diarrheal stool specimens.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographical variation in antimicrobial resistant Salmonella Schwarzengrund from chicken meat in Japan.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-24 DOI: 10.1292/jvms.24-0279

Yoshimasa Sasaki, Yoko Furuya, Shotaro Suzuki, Yoshika Momose, Masashi Uema, Mitsunori Kayano, Aikawa Chihiro, Mizuki Sasaki, Masashi Okamura, Kenji Ohya

引用次数: 0

Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-22 DOI: 10.1292/jvms.24-0296

Natsume Kurahara, Ayami Yutsudo, Osamu Yamato, Noriaki Miyoshi, Tatsuro Hifumi, Akira Yabuki

{"title":"Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease.","authors":"Natsume Kurahara, Ayami Yutsudo, Osamu Yamato, Noriaki Miyoshi, Tatsuro Hifumi, Akira Yabuki","doi":"10.1292/jvms.24-0296","DOIUrl":"https://doi.org/10.1292/jvms.24-0296","url":null,"abstract":"Apoptosis, an important pathological event associated with kidney disease progression, is expected to be a therapeutic target in chronic kidney disease (CKD). However, its role in naturally occurring CKD in aged cats remains unclear. Therefore, here, we investigated kidney tissues from aged cats (≥10 years) with or without azotemic CKD to evaluate apoptotic events using a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay. The positive TUNEL signals of the renal cells were quantified and statistically analyzed for correlation with the severity of plasma creatinine (pCre) concentration, renal lesions (glomerulosclerosis, interstitial cell infiltration, peritubular capillaries, and interstitial fibrosis), and oxidative damage of the kidney tissue. Oxidative damage was evaluated using immunohistochemistry for 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxynonenal (4-HNE). In the TUNEL assay, regardless of azotemia, positive nuclear signals were observed in the tubular epithelial and intraluminal cells, interstitial infiltrating cells, and glomerular cells. Quantitative TUNEL scores showed no significant differences between the azotemic and non-azotemic groups in any compartment of the kidney tissues. In the azotemic group, TUNEL scores did not correlate with pCre or renal lesion severity. However, the scores showed a significant positive correlation with the scores of 8-OHdG and 4-HNE. These findings suggest that apoptosis associated with oxidative damage in renal tissue is an initial pathological event that leads to CKD, rather than a change following CKD progression, in aged cats. Inhibiting apoptosis by antioxidant treatment may be a key strategy to prevent the development of CKD.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depot-specific adiposity changes in ovariectomized mice on high-fat diet.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-22 DOI: 10.1292/jvms.24-0442

Danang Dwi Cahyadi, Katsuhiko Warita, Yoshinao Z Hosaka

{"title":"Depot-specific adiposity changes in ovariectomized mice on high-fat diet.","authors":"Danang Dwi Cahyadi, Katsuhiko Warita, Yoshinao Z Hosaka","doi":"10.1292/jvms.24-0442","DOIUrl":"https://doi.org/10.1292/jvms.24-0442","url":null,"abstract":"Ovariectomized (OVX) mice serve as a key model for studying postmenopausal metabolic changes, particularly obesity, as they mimic the hormonal state of postmenopausal women. However, our understanding remains limited regarding how hormonal and dietary factors affect different adipose tissues. Furthermore, precise documentation of experimental procedures and their effects on specific adipose tissue depots is essential for reproducible translational research. This study investigated depot-specific adiposity development in OVX mice fed a high-fat diet (HFD), focusing on how reduced estrogen levels and dietary intervention affect distinct fat depots. We composed subcutaneous and visceral white adipose tissue (WAT) depots from sham-operated (Sham) and OVX female C57BL/6JJcl mice on a regular diet (RD) and high-fat diet (HFD) for 20 weeks. OVX mice on HFD gained significantly more weight than Sham controls. Adiposity increased in abdominal subcutaneous WAT (sWAT) and perirenal WAT (prWAT) of OVX mice, but not in mesenteric WAT (mWAT). Analysis of adipose tissue morphology revealed that OVX mice exhibited enlarged adipocyte cross-sectional areas under low estrogen (E2) conditions, suggesting enhanced adipogenesis in an estrogen-deficient state. These findings suggest that low estrogen condition accelerated adiposity, in a tissue site-dependent manner.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of storage conditions and periods for a three-drug anesthetic mixture in mice.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-21 DOI: 10.1292/jvms.24-0516

Hiroyuki Imai, Muneyoshi Hyoto, Kaoru Fujino, Sumito Matsuya, Akari Nishida, Ryoji Aoyagi, Yunosuke Tsutsumi, Kiyoshi Kano, Ken Takeshi Kusakabe

引用次数: 0

Magnetic resonance imaging and pathological findings of aortoiliac thromboembolism with a thoracic aortic mural thrombus in a Japanese Black calf.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-21 DOI: 10.1292/jvms.24-0407

Natsuki Akashi, Ayako Okada, Koutaro Nakamura, Takeshi Tsuka

引用次数: 0

Daily topical application of chlorhexidine gluconate to the skin in dogs and its impact on skin barriers and cytotoxicity. 每日局部应用葡萄糖酸氯己定在狗的皮肤及其对皮肤屏障和细胞毒性的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-16 DOI: 10.1292/jvms.24-0311

Akira Matsuda, Yukari Nakamura, Takamasa Itoi, Kiyotaka Arai, Kenji Kutara, Keisuke Sugimoto, Noritaka Maeta

{"title":"Daily topical application of chlorhexidine gluconate to the skin in dogs and its impact on skin barriers and cytotoxicity.","authors":"Akira Matsuda, Yukari Nakamura, Takamasa Itoi, Kiyotaka Arai, Kenji Kutara, Keisuke Sugimoto, Noritaka Maeta","doi":"10.1292/jvms.24-0311","DOIUrl":"https://doi.org/10.1292/jvms.24-0311","url":null,"abstract":"In recent years, the importance of using local disinfectants instead of systemic antibiotics for the treatment of infectious skin diseases to prevent the emergence of resistant bacteria has become widely recognized. Chlorhexidine gluconate (CHG) is commonly used in veterinary antibacterial shampoos; however, the daily topical application of diluted CHG solutions has also been adopted. Despite its widespread use, few studies have investigated the effects of CHG on the canine skin barrier. This study examined the skin barrier-damaging effects of CHG in dogs by applying diluted CHG (0.05%, 0.5%, and 4%) daily to six healthy dogs for 2 weeks. At the end of the trial, transepidermal water loss (TEWL), skin surface hydration (SSH), and number of skin bacteria were evaluated. Additionally, the cytotoxicity of CHG to the canine progenitor epidermal keratinocytes (CPEK) was examined using the water-soluble tetrazolium salt (WST-8) assay. Continuous application of 0.5% and 4% CHG to the skin led to a significant reduction in the number of skin bacteria. Additionally, at 4% CHG, a notable increase in TEWL and a decrease in SSH was observed. The WST assay revealed cytotoxicity of CHG at concentrations of 0.05%. In conclusion, although daily topical application of 4% CHG demonstrated the potential to disrupt the skin barrier, 0.5% CHG demonstrated sufficient antimicrobial activity without skin barrier disruption. Additionally, when treating dogs' skin with a compromised stratum corneum, use of lower concentration of CHG is suitable.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rare case of solitary intrahepatic splenosis following splenectomy in a dog. 一例罕见的狗脾切除术后单发肝内脾病。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-15 DOI: 10.1292/jvms.24-0080

Hiroshi Sunahara, Yuki Nemoto, Dito Anggoro, Melpa Susanti Purba, Kazuhito Itamoto, Harumichi Itoh, Munekazu Nakaichi, Kenji Tani

{"title":"A rare case of solitary intrahepatic splenosis following splenectomy in a dog.","authors":"Hiroshi Sunahara, Yuki Nemoto, Dito Anggoro, Melpa Susanti Purba, Kazuhito Itamoto, Harumichi Itoh, Munekazu Nakaichi, Kenji Tani","doi":"10.1292/jvms.24-0080","DOIUrl":"https://doi.org/10.1292/jvms.24-0080","url":null,"abstract":"A 9-year-old spayed female mixed breed dog weighing 6.8 kg with a history of previous splenectomy for hemangiosarcoma 4 years earlier was referred for a hepatic mass lesion. Although the dog did not have a clinical sign, a computed tomography revealed a solitary mass in the left medial lobe of the liver. Spleen-like tissue was observed on needle core biopsy of the mass, leading to the diagnosis of intrahepatic splenosis. Therefore, hepatic lobectomy of the left medial lobe of the liver was performed. Histopathological evaluation of the excised tumor confirmed the diagnosis of intrahepatic splenosis. No further development of splenosis was detected on CT performed 217 days after the surgery. The dog died of suspected aspiration pneumonia without any event or clinical signs related to intrahepatic splenosis on postoperative 272 days. The cause of the intrahepatic splenosis observed in this dog was unknown; however, hematogeneous dissemination and autoimplantation of splenic tissue via the portal venous flow was speculated. This case shows the utility of needle core biopsy for the preoperative diagnosis of splenosis. The intrahepatic splenosis in this case was considered to have developed as a long-term sequelae after splenectomy, as in humans.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective detection of ITGB7 gene mutation in a Holstein calf with chronic diarrhea that was suspected of hereditary cholesterol deficiency. 怀疑遗传性胆固醇缺乏的荷斯坦犊牛慢性腹泻患者ITGB7基因突变的回顾性检测

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-01-15 DOI: 10.1292/jvms.24-0392

Hisashi Inokuma, Masaki Maezawa, Gaku Tahara, Yoshiyuki Miyazaki, Atsushi Ogino, Ken-Ichi Watanabe, Yoshiyasu Kobayashi

引用次数: 0