Journal of Veterinary Medical Science最新文献_第10页

Nodal T-cell lymphoma with eosinophilic infiltration and sclerosing fibroplasia in a cat with eosinophilia. 一只患有嗜酸性粒细胞增多症的猫患上了伴有嗜酸性粒细胞浸润和硬化性纤维增生的结节性 T 细胞淋巴瘤。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI: 10.1292/jvms.24-0225

Tamami Suzuki, Jumpei Yamazaki, Kouta Yamaguchi, Keisuke Aoshima, Takashi Kimura

引用次数: 0

Attenuation of the neuropathogenic equine herpesvirus type 1 strain Ab4p in hamsters by a single amino acid mutation (D752N) in viral DNA polymerase ORF30. 病毒DNA聚合酶ORF30中的一个氨基酸突变（D752N）对仓鼠神经致病性马疱疹病毒1型毒株Ab4p的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-09 DOI: 10.1292/jvms.24-0338

Fuka Nishimura, Noriko Fukushi, Hiroki Sakai, Hideto Fukushi

引用次数: 0

Prevalence of pathogens and antimicrobial resistance of isolated Staphylococcus spp. in bovine mastitis milk in South Korea, 2018-2022. 2018-2022 年韩国牛乳腺炎牛奶中分离出的葡萄球菌的病原体流行率和抗菌药耐药性。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-11 DOI: 10.1292/jvms.24-0239

Hye Jeong Kang, Ju-Yeon You, Serim Hong, Jin-San Moon, Ha-Young Kim, Ji-Hye Choi, Jae-Myoung Kim, Young Ju Lee, Hyun-Mi Kang

{"title":"Prevalence of pathogens and antimicrobial resistance of isolated Staphylococcus spp. in bovine mastitis milk in South Korea, 2018-2022.","authors":"Hye Jeong Kang, Ju-Yeon You, Serim Hong, Jin-San Moon, Ha-Young Kim, Ji-Hye Choi, Jae-Myoung Kim, Young Ju Lee, Hyun-Mi Kang","doi":"10.1292/jvms.24-0239","DOIUrl":"10.1292/jvms.24-0239","url":null,"abstract":"Staphylococcus spp. are one of the most predominant isolates in milk samples of dairy cows with mastitis worldwide. The aims of this study were to investigate the prevalence of bacterial pathogens in bovine mastitis milk samples in South Korea and the antimicrobial resistance profiles of staphylococcal isolates. In total, 1,245 strains were isolated from 1,260 mastitis quarter milk samples (with somatic cell counts ≥ 200,000 cells/mL) from 66 dairy farms between 2018 and 2022. The bacterial genus with the highest prevalence in bovine mastitis milk samples was Staphylococcus spp. (33.9%), followed by Streptococcus spp. (11.5%). S. aureus and non-aureus staphylococci (NAS) accounted for 11.0% and 89.0% of staphylococcal isolates, respectively. S. chromogenes was the most prevalent species among the 22 NAS species detected. S. aureus showed the highest resistance rates to penicillin (25.0%) and ampicillin (20.8%), whereas NAS showed the highest resistance rates to penicillin (18.3%), tetracycline (11.4%) and erythromycin (10.1%). Sixteen multidrug-resistant (MDR) isolates were only isolated from NAS, and the most commonly detected antimicrobial resistance gene in the 16 MDR isolates was mecA (75.0%), followed by tetK (62.5%), blaZ (50.0%), ermC (50.0%), and lnuA (43.8%). In conclusion, NAS were the most common isolates from mastitis milk in South Korea and MDR isolates carried a variety of antibiotic resistance genes. Our study suggests that continuous monitoring of the distribution and antimicrobial resistance in Staphylococcus spp., particularly NAS, is needed to improve the effectiveness of management and treatment strategies in dairy farms.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1219-1226"},"PeriodicalIF":1.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142479252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salinomycin induces apoptosis and potentiates the antitumor effect of doxorubicin against feline mammary tumor 2.5D organoids. 盐霉素诱导细胞凋亡并增强多柔比星对猫乳腺肿瘤2.5D器官组织的抗肿瘤作用。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI: 10.1292/jvms.24-0344

Yishan Liu, Mohamed Elbadawy, Haru Yamamoto, Amira Augomaa, Yusuke Ishihara, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki

{"title":"Salinomycin induces apoptosis and potentiates the antitumor effect of doxorubicin against feline mammary tumor 2.5D organoids.","authors":"Yishan Liu, Mohamed Elbadawy, Haru Yamamoto, Amira Augomaa, Yusuke Ishihara, Masahiro Kaneda, Tatsuya Usui, Kazuaki Sasaki","doi":"10.1292/jvms.24-0344","DOIUrl":"10.1292/jvms.24-0344","url":null,"abstract":"Feline mammary tumors (FMT) are the third most common form of neoplasm in cats. The prognosis of FMT is poor due to its high malignancy and metastatic potential. The outcomes of treatment using the common anticancer drug doxorubicin (DOX) are unsatisfactory, with resistance inevitably leading to treatment failure and disease recurrence. Salinomycin (SAL), an antibiotic, has been reported to exert anticancer effects on both human and canine mammary tumors. To recapitulate the genetic and molecular imprints of the original tumor sample, we generated four strains of patient-derived FMT 2.5D organoids (FMTO) to examine the anti-tumor potential of SAL. Our results revealed that SAL decreased cell viability in a dose-dependent manner. Treatment of FMTO with SAL-induced cell apoptosis, represented by an upregulation of P21, Caspase-8, and Caspase-9, and increased activity of Caspase-3/7. The combination of low-dose SAL with DOX (SD) potentiated the cytotoxicity of the latter in both DOX-resistant and DOX-sensitive strains, promoting cell apoptosis and cell-cycle arrest. In vivo, experiments using FMTO-derived xenografts engrafted into mice revealed decreased tumor growth following SAL administration. In conclusion, SAL showed anticancer activity against FMTO and potentiated the anticancer effect of DOX by inhibiting cell proliferation and inducing apoptosis and cell cycle arrest. These results suggest that SAL may represent a new adjuvant treatment option for patients with FMT.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1256-1264"},"PeriodicalIF":1.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11612252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elephant endotheliotropic herpesvirus gB-specific antibody levels in sera of Asian elephants (Elephas maximus) in Japanese zoos. 日本动物园亚洲象（Elephas maximus）血清中大象内皮细胞疱疹病毒 gB 特异性抗体水平。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-12-01 Epub Date: 2024-10-16 DOI: 10.1292/jvms.23-0503

Kazuya Takehana, Tabitha E Hoornweg, Willem Schaftenaar, Victor Pgm Rutten, Cornelis Am DE Haan, Keita Matsuno

引用次数: 0

Parasitological and molecular investigation of Trypanosoma evansi in dromedaries from Greater Cairo, Egypt. 埃及大开罗地区单峰驼中伊万斯锥虫的寄生虫学和分子研究。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-11-15 Epub Date: 2024-10-02 DOI: 10.1292/jvms.24-0284

Moaz M Amer, Ahmed M Soliman, Thom DO, Asmaa Abdelwadod Hegab, Eman Ahmed El-Kelesh, Yongchang Li, Jerzy Jaroszewski, Uday Kumar Mohanta, Xuenan Xuan

{"title":"Parasitological and molecular investigation of Trypanosoma evansi in dromedaries from Greater Cairo, Egypt.","authors":"Moaz M Amer, Ahmed M Soliman, Thom DO, Asmaa Abdelwadod Hegab, Eman Ahmed El-Kelesh, Yongchang Li, Jerzy Jaroszewski, Uday Kumar Mohanta, Xuenan Xuan","doi":"10.1292/jvms.24-0284","DOIUrl":"10.1292/jvms.24-0284","url":null,"abstract":"In Egypt, camel trypanosomiasis is widespread. From October 2021 to March 2022, we collected 181 blood samples from apparently healthy one-humped camels (Camelus dromedarius) in Cairo and Giza Governates. The objective of this study was to assess infection rates of trypanosomes using blood smear examination and PCR-sequencing assays. Trypanosomes were detected in 8.3% (15/181) of camels by blood smear and in 23.8% (43/181) by PCR targeting the internal transcribed spacer (ITS). Based on blood smear and ITS-PCR results, and the absence of tsetse flies in the study area, we hypothesized that the Trypanosoma species was likely T. evansi. Validation using PCR based on the variant surface glycoprotein (VSG) of T. evansi Rode Trypanozoon antigen type (RoTat) 1.2 (RoTat 1.2 VSG gene) on ITS-PCR-positive samples (n=43) confirmed that 88.4% (38/43) were RoTat 1.2 T. evansi, while 11.6% (5/43) were non-RoTat 1.2 T. evansi. This marks the second report of non-RoTat 1.2 T. evansi in dromedary camels in Egypt. Considering the underestimated zoonotic risk of T. evansi in Egypt, there is a potential threat to humans, underscoring the need for a \"One Health\" approach to safeguard animal and human health.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1177-1184"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebrospinal fluid flow in small-breed dogs with idiopathic epilepsy observed using time-spatial labeling inversion pulse images: a preliminary study. 使用时空标记反转脉冲图像观察患有特发性癫痫的小型犬脑脊液流动：初步研究。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-11-15 Epub Date: 2024-10-03 DOI: 10.1292/jvms.23-0305

Chieko Ishikawa, Natsumi Tanaka, Naoki Sekiguchi, Masato Kitagawa, Daisuke Ito

{"title":"Cerebrospinal fluid flow in small-breed dogs with idiopathic epilepsy observed using time-spatial labeling inversion pulse images: a preliminary study.","authors":"Chieko Ishikawa, Natsumi Tanaka, Naoki Sekiguchi, Masato Kitagawa, Daisuke Ito","doi":"10.1292/jvms.23-0305","DOIUrl":"10.1292/jvms.23-0305","url":null,"abstract":"Cerebrospinal fluid (CSF) circulation diseases, such as hydrocephalus and syringomyelia, are common in small-breed dogs. In human patients with CSF circulation diseases, time-spatial labeling inversion pulse (time-SLIP) sequence performed to evaluate CSF flow before and after treatment allows visualization of the restoration of CSF movement. However, studies evaluating CSF flow using the time-SLIP method in small-breed dogs are limited. Therefore, the present study aimed to evaluate intracranial CSF flow on time-SLIP images in small-breed dogs with idiopathic epilepsy, as an alternative model to healthy dogs. Time-SLIP images were obtained at two sites: 1) the mesencephalic aqueduct (MA) area (third ventricle, MA, and brain-base subarachnoid space [SAS]) and 2) the craniocervical junction area (fourth ventricle, brainstem, and cervical spinal cord SAS) to allow subsequent evaluation of the rostral and caudal CSF flow using subjective and objective methods. In total, six dogs were included. Caudal flow at the MA and brain-base SAS and rostral flow in the brainstem SAS were subjectively and objectively observed in all and 5/6 dogs, respectively. Objective evaluation revealed that a significantly smaller movement of the CSF, assessed as the absence of CSF flow by subjective evaluation, could be detected in some areas. In small-breed dogs, the MA, brain-base, and brainstem SAS would be appropriate areas for evaluating CSF movement, either in the rostral or caudal flows on time-SLIP images. In areas where CSF movement cannot detected by subjective methods, an objective evaluation should be conducted.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1168-1176"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a novel ephemerovirus in a water buffalo (Bubalus bubalis [Linnaeus, 1758]). 在一头水牛（Bubalus bubalis [Linnaeus, 1758]）身上鉴定出一种新型附病毒。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-11-15 Epub Date: 2024-10-08 DOI: 10.1292/jvms.24-0295

Sakiho Imai, Mai Kishimoto, Masayuki Horie

{"title":"Identification of a novel ephemerovirus in a water buffalo (Bubalus bubalis [Linnaeus, 1758]).","authors":"Sakiho Imai, Mai Kishimoto, Masayuki Horie","doi":"10.1292/jvms.24-0295","DOIUrl":"10.1292/jvms.24-0295","url":null,"abstract":"Ephemeroviruses, belonging to the genus Ephemerovirus within the family Rhabdoviridae of the Mononegavirales, are non-segmented, negative-strand RNA viruses that infect artiodactyls and blood-sucking arthropods. Although recent advances in sequencing technology have facilitated the identification of novel ephemeroviruses, thereby expanding our understanding of this viral genus, their diversity remains elusive, as evidenced by phylogenetic gaps between currently known ephemeroviruses. In this study, we analyzed publicly available RNA-seq data and identified a novel ephemerovirus, tentatively named Punjab virus (PBV), in a water buffalo (Bubalus bubalis [Linnaeus, 1758]). We obtained two separate PBV contigs from the RNA-seq data; the first contig covers the N, P, and M genes, while the second contig covers the G, α, β, γ, and L genes. Together, these PBV contigs represent 99% of the estimated complete viral genome. Mapping analysis revealed a typical transcriptional gradient pattern commonly observed in mononegaviruses, suggesting that the water buffalo is the authentic host for PBV. Sequence comparisons with its closest relatives indicate that the newly identified virus meets the ICTV species demarcation criteria for sequence divergence. Thus, this study contributes to a deeper understanding of the diversity of ephemeroviruses.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1205-1211"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142394808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic investigation of glycosylphosphatidylinositol (GPI) anchored Bd37 orthologs in Babesia divergens group and potential use of recombinant protein for ecological survey in deer. 糖基磷脂酰肌醇（GPI）锚定 Bd37 同源物的遗传学调查和重组蛋白在鹿生态调查中的潜在用途。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-11-15 Epub Date: 2024-10-07 DOI: 10.1292/jvms.24-0116

Aya Zamoto-Niikura, Katsuro Hagiwara, Koichi Imaoka, Shigeru Morikawa, Ken-Ichi Hanaki

{"title":"Genetic investigation of glycosylphosphatidylinositol (GPI) anchored Bd37 orthologs in Babesia divergens group and potential use of recombinant protein for ecological survey in deer.","authors":"Aya Zamoto-Niikura, Katsuro Hagiwara, Koichi Imaoka, Shigeru Morikawa, Ken-Ichi Hanaki","doi":"10.1292/jvms.24-0116","DOIUrl":"10.1292/jvms.24-0116","url":null,"abstract":"The Babesia divergens/B. capreoli group includes parasites with confirmed or possible zoonotic potential to cause human babesiosis. Currently, diagnostic antigen of the group has not been established. In this study, we investigated the ortholog of Bd37, a glycosylphosphatidylinositol (GPI)-anchored major merozoite surface protein of B. divergens sensu stricto, in the Asia lineage of the group. From two genomic isolates from sporozoite/sporoblast stages, three Bd37 gene variants, namely Bd37 JP-A, JP-B, and JP-C, were isolated with 62.3-64.1% amino acid sequence identity. Discriminative blood direct PCR revealed that Bd37 JP-A was encoded in all parasites infecting wild sika deer examined (n=22). While Bd37 JP-B and JP-C genes were randomly detected in 12 and 11 specimens, respectively. Sequencing of all JP-A variants revealed that the gene was polymorphic, with a low ratio of non-synonymous to synonymous substitutions (dN/dS) and that a highly polymorphic region was not related to predicted B-cell epitopes. A recombinant JP-A-based ELISA showed an overall positive rate of 13.9% in sika deer in Japan from north (Hokkaido) to south (Kyushu island) across 24 prefectures (n=360). This positive rate was twice as high as that examined by 18S rRNA-based PCR (6.6%). The geographical trends in infection rates were consistent. This study demonstrated that direct examination was informative for revealing genetic background and selecting antigen candidates. Bd37 orthologs may serve diagnostic purposes in combination with indirect fluorescence assay, which requires biological isolates.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1185-1197"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The composition of early lactation milk in recipient dairy cows determines success in bovine embryo transfer. 受体奶牛的早期泌乳乳汁成分决定了牛胚胎移植的成功与否。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-11-15 Epub Date: 2024-09-27 DOI: 10.1292/jvms.24-0292

Toshihiro Takano, Akira Goto, Toshihisa Sugino

{"title":"The composition of early lactation milk in recipient dairy cows determines success in bovine embryo transfer.","authors":"Toshihiro Takano, Akira Goto, Toshihisa Sugino","doi":"10.1292/jvms.24-0292","DOIUrl":"10.1292/jvms.24-0292","url":null,"abstract":"To identify new criteria for selecting recipient dairy cows for embryo transfer (ET), we retrospectively examined the effects of the composition of early lactation milk on fertility risk in ET. This study investigated the association between milk fat (FAT), milk protein (PRO), and milk urea nitrogen (MUN) levels during early lactation, based on production records, and subsequent fertility risk in ET using contingency table analysis and multivariable logistic regression analysis, which included five confounding variables. The results showed that MUN levels during early lactation were negatively associated with fertility risk in ET, while FAT and PRO levels showed no clear association. A reduction in MUN levels during the peak lactation period suggests a deficiency in dry matter intake, an inadequate protein supply, and an imbalance in the ratio of proteins to fermentable carbohydrates in the rumen, which may have adversely impacted fertility risk in ET. Monitoring MUN levels is crucial for maintaining a proper protein balance. The results obtained in this study suggest that MUN levels in the early lactation phase obtained from production records can be used as a predictor of fertility in recipients to improve the fertility risk in ET. No special techniques or costs are required for using production records, making them easy to use in clinical practice. Our findings provide valuable insights for optimizing cost-effectiveness and fertility risk in ET and their clinical applications.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1198-1204"},"PeriodicalIF":1.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11569873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0