Journal of Veterinary Medical Science最新文献

{"title":"Thermal antinociceptive effect of transdermal tramadol hydrochloride in avian.","authors":"Chien Yee Lim, Soon Heng Goh, Li Peng Tan, Krishna Veni Veloo, Wee Kiat Ong, Shen Fung Yong, Chien Joo Lim, Siew Shean Choong","doi":"10.1292/jvms.25-0009","DOIUrl":"10.1292/jvms.25-0009","url":null,"abstract":"<p><p>Drug administration in avian species via the parenteral route tends to result in stress, as most often birds need to be handled and manipulated. The purpose of this study was to evaluate the potential of the transdermal administration route as an alternative for tramadol hydrochloride in avian. A total of 24 85-day-old broiler chickens were used as a model for avian species in this study. The chickens were divided into four equal groups of 6 animals each: i. Control, without intervention (C); ii. Intravenous at 5 mg/kg (IV); iii. Transdermal at 5 mg/kg (TD1); and iv. Transdermal at 10 mg/kg (TD2). The thermal antinociception effect was opted to simulate pain, by using a heating plate. The foot withdrawal response of each animal to a thermal stimulus was determined pre-administration (as baseline), followed by 30-, 60-, 120-, 240-, 360- and 480-min post-tramadol administration. All treatment groups showed significantly higher (P<0.05) foot withdrawal temperature threshold compared to the control group. Comparatively, there was a statistically significant higher temperature threshold than baseline at 60-, and 120-min post treatment for both TD1 and TD2 groups, while the IV group only at 60-min post treatment. Intravenous tramadol recorded the highest change in the foot withdrawal thermal threshold, while transdermal tramadol at 10 mg/kg recorded the longest effective time up to 240 min post-administration. This is the first study to evaluate the analgesic effectiveness of transdermal tramadol in avian, thus further investigation is required to confirm similar effects in other species.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"947-953"},"PeriodicalIF":1.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative visualization of feline liposarcoma using short-wave infrared fluorescence imaging and indocyanine green.","authors":"Jihun Kim, Yujin Kim, Kyung-Mee Park, Sungin Lee","doi":"10.1292/jvms.25-0113","DOIUrl":"10.1292/jvms.25-0113","url":null,"abstract":"<p><p>A 9-year-old domestic short hair cat presented with a firm thoracic mass suspected to be a lipoma. Preoperative indocyanine green was administered intravenously at a dose of 5 mg/kg for short-wave infrared fluorescence imaging. Intraoperatively, a plaque-like lipoma overlaid a subjacent mass that exhibited strong fluorescence. Both were excised, and histopathology confirmed the subjacent mass as a well-differentiated liposarcoma. The cat recovered uneventfully with no recurrence at 11 months. This case highlights the utility of short-wave infrared fluorescence imaging in distinguishing benign from malignant tissues intraoperatively and enabling precise resection. To our knowledge, this is the first report of feline liposarcoma identified through short-wave infrared fluorescence imaging, demonstrating its potential as a valuable tool in veterinary oncology.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"894-897"},"PeriodicalIF":1.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of haemosporidian parasite lineages from wild bird carcasses collected in eastern Japan.","authors":"Momoko Eki, Mizuki Sasaki, Yasukazu Muramatsu, Mari Kobayashi, Takanori Ishikawa, Leo Uchida","doi":"10.1292/jvms.24-0267","DOIUrl":"10.1292/jvms.24-0267","url":null,"abstract":"<p><p>Avian haemosporidia are a group of vector-borne hemoparasites that are non-pathogenic to native bird hosts; however, some species/lineages cause severe disease or death in poultry and non-native birds. We investigated haemosporidian parasites in wild bird carcasses collected from four prefectures in eastern Japan. DNA was extracted from the liver, and the haemosporidian cytochrome b gene was amplified by nested PCR followed by direct sequencing. Of the 40 bird samples belonging to 25 species, 12 haemosporidian lineages were detected: four from Plasmodium, one from Haemoproteus, and seven from Leucocytozoon. Of these 12, two Leucocytozoon lineages were suspected to be novel. Leucocytozoon lineage TUMER02 was first detected in Japan. Six lineages were detected among the six novel host bird species.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"838-842"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of African pygmy hedgehog adenovirus 1 in exotic companion animals.","authors":"Iori Koizumi, Chisato Shigenaga, Kazuki Kiuno, Saki Mitsunaga, Miyuka Nishizato, Kota Nochide, Daisuke Hayasaka, Hiroshi Shimoda","doi":"10.1292/jvms.25-0164","DOIUrl":"10.1292/jvms.25-0164","url":null,"abstract":"<p><p>African pygmy hedgehog adenovirus 1 (AhAdV-1) was first identified in 2020 from a colony of African pygmy hedgehogs that succumbed to severe bronchopneumonia in Japan. AhAdV-1 is closely related to Skunk adenovirus 1, which was isolated from a wild skunk with acute hepatitis and pneumonia in Canada in 2015. Similar viruses have been isolated or detected in a diverse range of animals across multiple countries. While adenoviruses are generally considered highly species-specific, the host range of AhAdV-1 remains unclear. This study aimed to investigate the potential host range of AhAdV-1 through serological surveillance among 17 exotic animal species that visited a veterinary hospital in Fukuoka, Japan. Neutralizing antibodies against AhAdV-1 were detected in several species, with particularly high seroprevalence observed in meerkats (41%), ferrets (62%), and African pygmy hedgehogs (63%). Notably, ferrets and hedgehogs with a history of respiratory symptoms exhibited significantly higher seroprevalence compared to asymptomatic individuals. These findings suggest a broad host range for AhAdV-1, with certain species showing high exposure rates. Further investigations are needed to determine the sources and transmission routes of AhAdV-1, as well as its potential for zoonotic infection.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"862-867"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of mesenchymal stem cells as feeder cells for the cultivation of corneal epithelial cell sheets in dogs.","authors":"Maresuke Morita, Naoki Fujita, Takayuki Nakagawa, Ryohei Nishimura, Keiko Tsuzuki","doi":"10.1292/jvms.24-0532","DOIUrl":"10.1292/jvms.24-0532","url":null,"abstract":"<p><p>Corneal epithelial cell sheet transplantation is a promising therapy for reconstructing the ocular surface in canines with severe corneal disease. Cultivation of these sheets typically requires feeder cells that support the proliferation and maintenance of immature epithelial cells by secreting growth factors. However, using conventional murine 3T3 fibroblasts as feeder cells raises ethical and safety concerns. This study investigated canine adipose-derived mesenchymal stem cells (cADMSCs) and bone marrow mesenchymal stem cells (cBMMSCs) as alternative feeder cells. Both MSCs were cultured alone or with canine corneal epithelial cells. Changes in epidermal growth factor, keratinocyte growth factor, and hepatocyte growth factor (HGF) expression in both MSC types were evaluated by quantitative polymerase chain reaction. HGF expression was further assessed by western blot and enzyme-linked immunosorbent assay. Canine corneal epithelial cell sheets were cultivated with cADMSCs, cBMMSCs, or 3T3 fibroblasts, or without feeder cells, and their structure and quality were evaluated histopathologically. Results showed that cADMSCs and cBMMSCs originally expressed soluble factors, and HGF mRNA expression significantly upregulated when co-cultured with epithelial cells. However, no significant differences in sheet structure or quality were observed among feeder types. In conclusion, cADMSCs and cBMMSCs are viable alternatives to 3T3 fibroblasts for cultivating canine corneal epithelial cell sheets. Canine corneal epithelial cells interact with MSCs and promote the secretion of soluble factors from MSCs to promote epithelial cell growth. Moreover, canine corneal epithelial cells may maintain their proliferative capacity and immature state independent of feeder cells.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"843-853"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-element analysis of Traditional Mongolian Medicine Ga-Mu-Zhu-Er using inductively coupled plasma mass spectrometry.","authors":"Qin Na, Gu-Leng Amu, Li Mei, Zhi Gui, Lin Song","doi":"10.1292/jvms.24-0414","DOIUrl":"10.1292/jvms.24-0414","url":null,"abstract":"<p><p>Due to the high level of heavy metals and harmful elements, United States and Europe are precautious in importing herbal medicines, which has seriously hindered the internationalization process of traditional Chinese and Mongolian medicines. Ga-Mu-Zhu-Er (GMZE) contains a wide range of chemical compounds and inorganic elements. This paper describes a validation process for the simultaneous analysis of 30 elements in Traditional Mongolian Medicine GMZE samples with closed-vessel microwave digestion technique followed by inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The samples digested with concentrated nitric acid (65%, v/v) revealed recoveries ranging from 91.9 to 107.7%. Several other parameters, including linearity, specificity, the limit of detection (LOD) and quantification (LOQ), precision of measurement, repeatability, intermediate precision and accuracy, were validated. The LOD were found to be in the range of 0.00015-0.21 µg/L, and the LOQ were between 0.00046 to 0.64 µg/L. Three GMZE samples were analyzed. Among the analyzed elements, Ca, As, and Hg were the most abundant, whereas, Be and Ag were present in low concentrations in all the samples. The results obtained in our study demonstrated that the developed ICP-MS method is simple, fast, and reliable, which could be used for the routine quantitation of these 30 essential and non-essential trace elements in GMZE as well as other Traditional Chinese Medicines and dietary supplements, with acceptable analytical performance.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"854-861"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isorhamnetin ameliorates ovariectomy-induced osteoporosis in female rats by regulating the receptor activator of nuclear factor kappa B ligand, osteoprotegerin, bone morphogenetic protein 2 and runt-related transcription factor 2 signaling pathway.","authors":"Haibin Jiao, Kun Zheng","doi":"10.1292/jvms.25-0006","DOIUrl":"10.1292/jvms.25-0006","url":null,"abstract":"<p><p>Osteoporosis is characterized by reduced bone density and increased fracture risk. The present study assessed anti-osteoporotic effects of isorhamnetin on ovariectomy (OVX)-induced osteoporosis in female rats. Osteoporosis was induced in OVXX-female Sprague-Dawley rats by using Freund's Complete Adjuvant and randomly divided into the following groups (n=15): OVX control, alendronate (3 mg/kg, subcutaneous), and isorhamnetin (10, 20, and 40 mg/kg, p.o.), and received treatment for five weeks after OVX. In results following OVX, significant alterations in behavioral, biochemical, and histological parameters were observed. Conversely, isorhamnetin (20 and 40 mg/kg) treatment significantly improved (P<0.05) OVX-induced alterations in body, femur, and uterine weight, bone mineral content and density, but also effectively mitigated (P<0.05) elevated allodynia and hyperalgesia. It notably improved (P<0.05) changes in serum alkaline phosphatase, osteocalcin, C-terminal telopeptide of type I collagen (CTX-I), serum and urinary calcium, and phosphorus levels. Isorhamnetin markedly attenuated elevated (P<0.05) serum TNF-α, IL-1β, IL-6 levels but increased (P<0.05) serum IL-4 and IL-10 levels. Furthermore, mRNA expression of osteoprotegerin (OPG), runt-related transcription factor 2 (Runx2), and bone morphogenetic protein 2 (BMP-2) was upregulated (P<0.05), whereas receptor activator of nuclear factor kappa B ligand (RANKL) was downregulated (P<0.05). Histological analysis demonstrated that isorhamnetin effectively improved (P<0.05) OVX-induced inflammation, thereby preventing cellular infiltration, synovial hyperplasia, cartilage erosion, and pannus formation in bone specimens. In conclusion, isorhamnetin exerts its anti-osteoporotic potential by modulating pain (allodynia and hyperalgesia), serum biomarkers (osteocalcin, CTX-I, TNF-α, ILs), and bone signaling pathways (RANKL, OPG, BMP-2, Runx2).</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"826-837"},"PeriodicalIF":1.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No evidence of bovine leukemia virus proviral DNA in a widely used commercially frozen semen in Japan.","authors":"Aronggaowa Bao, Sonoko Watanuki, Ryosuke Matsuura, Yasunobu Matsumoto, Hiroyuki Shimizu, Ryusaku Kawata, Yoko Aida","doi":"10.1292/jvms.25-0212","DOIUrl":"10.1292/jvms.25-0212","url":null,"abstract":"<p><p>Bovine leukemia virus (BLV) is a global concern that significantly affects the productivity of dairy and beef cattle, leading to considerable economic losses. BLV is primarily transmitted through infected lymphocytes via both horizontal and vertical routes. Vertical transmission occurs through the colostrum, uterus, and placenta. Although the possibility of BLV transmission through infected semen during artificial insemination is a recognized concern. No such cases have been reported in Japan, while studies from other countries reported a low risk. In this study, we aimed to evaluate the presence of BLV provirus in commercially frozen semen to assess the potential risk of infection in Japan. A sufficient quantity of genomic DNA with adequate purity was extracted from 191 widely used commercially frozen semen samples obtained from top-ranking bulls in the annual market survey in Japan between 2000 and 2022 using the phenol-chloroform extraction method. No BLV provirus was detected in any of the 191 frozen semen samples using either BLV-CoCoMo-qPCR-2 targeting the BLV long-terminal repeat region or nested polymerase chain reaction targeting BLV gp51 env gene. Similarly, the results are consistent with reports from other countries. Therefore, the findings of this study suggest a low risk of BLV transmission through frozen semen in Japan. Our study further supports that artificial insemination combined with the selection of non-infected cows for breeding are effective measures to prevent the spread of infection.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"821-825"},"PeriodicalIF":1.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of birth month on endocrine function in Thoroughbred foals born in Hokkaido, the northern part of Japan.","authors":"Mutsuki Ishimaru, Koichi Kume, Harutaka Murase, Fumio Sato, Akira Matsui, Hajime Ohmura, Kazuyoshi Taya","doi":"10.1292/jvms.25-0061","DOIUrl":"10.1292/jvms.25-0061","url":null,"abstract":"<p><p>Thoroughbreds born early in the year are believed to have an advantage in racing. However, the development of endocrine functions in early-born foals in the cold winter climate is unclear. The present study was conducted to elucidate the effect of birth month on the development of endocrine function in Thoroughbred foals born from February to May in Hokkaido by comparing hypothalamic-pituitary axis, adrenal and thyroid functions from birth to 24 weeks of age. Eighty-seven Thoroughbred foals were used to compare the changes in circulating luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, cortisol, and thyroxine among four birth month groups for each sex. The present study revealed that as foals grow, the hypothalamic-pituitary axis develops, allowing them to secrete LH and FSH in response to prolonged day length from about 10 weeks of age, regardless of birth month for both sexes. In addition, the increase in prolactin concentrations from May to August suggests that postnatal foals may have the ability to respond to prolonged daylength immediately after birth, independent of growth. Furthermore, circulating cortisol and thyroxine in the foals at birth were elevated regardless of birth month, suggesting that adrenal and thyroid functions were fully activated for the rapid growth during late gestation and adaptation to the external environment that occurs early postnatal period. In early-born foals, these hormone concentrations were found to be within the normal range, indicating that development of foals were not affected by the harsh Hokkaido winter.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"804-815"},"PeriodicalIF":1.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective study on reproduction in alpacas (Lama pacos) and cria growth after birth in Japan: the relationship between parturition and climate conditions.","authors":"Misa Arafurue, Chihiro Kanno, Shogo Sato, Yosuke Maeda, Fumiaki Takahashi, Kenji Momozawa, Masashi Nagano","doi":"10.1292/jvms.25-0200","DOIUrl":"10.1292/jvms.25-0200","url":null,"abstract":"<p><p>Alpaca breeding is affected by environmental factors and management on farms. However, this has not yet been examined in detail in Japan. Therefore, we herein investigated the relationship between parturition in 65 alpacas on a Japanese farm and environmental factors. Parturition was observed every month, except for December, and the survival rate of crias was 81.5% (53/65). The gestation length was significantly longer for surviving crias than for dead crias (352.3 ± 17.9 vs. 338.7 ± 20.8 days, P<0.05). Birth weight was higher in surviving crias than in dead crias (6.7 ± 1.1 vs. 5.0 ± 1.0 kg, P<0.05). An analysis of the relationship between parturition and climate revealed a correlation between low ambient temperature one month before parturition and higher birth weights in surviving crias.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"816-820"},"PeriodicalIF":1.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}