Journal of Veterinary Medical Science最新文献_第9页

Blood chloride abnormalities in diarrheic neonatal calves with metabolic acidosis. 患有代谢性酸中毒的腹泻新生犊牛血氯异常。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-27 DOI: 10.1292/jvms.24-0089

Kenji Tsukano, Shohei Yamakawa, Kazuyuki Suzuki

{"title":"Blood chloride abnormalities in diarrheic neonatal calves with metabolic acidosis.","authors":"Kenji Tsukano, Shohei Yamakawa, Kazuyuki Suzuki","doi":"10.1292/jvms.24-0089","DOIUrl":"10.1292/jvms.24-0089","url":null,"abstract":"The present study investigated the prevalence of blood chloride (Cl) abnormalities in diarrheic neonatal calves with metabolic acidosis and attempted to identify the most relevant electrolyte abnormality to these abnormalities. A retrospective analysis was conducted on the medical records of 157 diarrheic neonatal calves aged 10.3 ± 4.2 days old with metabolic acidosis. Hypochloremia, normochloremia, and hyperchloremia were observed in 8.9% (14/157), 43.3% (68/157), and 47.8% (68/157), respectively, of diarrheic calves with metabolic acidosis. This distribution remained similar regardless of age (under 8 days or 8 days and older). Furthermore, a multiple logistic regression analysis showed that variations in values for blood sodium [Na (regression coefficients 0.877; 95% confidence interval (CI) 13.977-134.195; P<0.01)], pH (regression coefficients -10.719; 95% CI -19.076- -2.362; P<0.05), and bicarbonate [HCO3- (regression coefficients -0.555; 95% CI -0.820- -0.290; P<0.01)] were associated with blood Cl abnormalities. The present results revealed that blood Na concentrations were more strongly associated with blood Cl concentrations than blood pH and HCO3- values. In the present study, diarrheic calves with hyperchloremia were characterized by normonatremia and extremely severe metabolic acidosis.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the Wnt/β-catenin signaling pathway and CTNNB1 mutations in canine intestinal epithelial proliferative lesions. 犬肠道上皮增生性病变中 Wnt/β-catenin 信号通路的激活和 CTNNB1 突变。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-29 DOI: 10.1292/jvms.24-0125

Kento Ishikawa, James K Chambers, Kazuyuki Uchida

{"title":"Activation of the Wnt/β-catenin signaling pathway and CTNNB1 mutations in canine intestinal epithelial proliferative lesions.","authors":"Kento Ishikawa, James K Chambers, Kazuyuki Uchida","doi":"10.1292/jvms.24-0125","DOIUrl":"10.1292/jvms.24-0125","url":null,"abstract":"Nuclear expression of β-catenin has been reported in canine intestinal epithelial tumors (IETs) and colorectal inflammatory polyps (CIPs) with dysplastic epithelia. However, the role of the Wnt/β-catenin signaling pathway in these lesions remains unclear. To investigate the association between the nuclear β-catenin expression and the activation of the Wnt/β-catenin signaling pathway, immunohistochemistry and mutation analyses were conducted on 64 IETs and 20 CIPs. IETs and CIPs with β-catenin nuclear and/or cytoplasm immunolabeling were classified as β-catenin (+). The immunostaining scores of c-Myc and Cyclin D1 and Ki-67 index were significantly higher in β-catenin (+) cases than in β-catenin (-) cases. Identical APC mutations (p.E154D and p.K155X) were detected in 6/41 β-catenin (+) IETs; all 6 of IETs with APC mutations were Jack Russell Terriers. CTNNB1 mutations were detected in 29/42 β-catenin (+) IETs, 3/11 β-catenin (+) CIPs, and 2/22 β-catenin (-) IETs, most of which were hotspots associated with human colorectal carcinoma. In one Miniature Dachshund diagnosed with a CIP that subsequently developed into an IET, the same CTNNB1 mutation was detected in both lesions. The immunohistochemical results suggest that cell proliferative activity in β-catenin (+) cases may be associated with activation of the Wnt/β-catenin signaling pathway. The mutation analysis results suggest that CTNNB1 mutations may be associated with cytoplasmic β-catenin accumulation in IET and CIP. Furthermore, the dysplastic epithelium in CIP may progress to IET through the activation of the Wnt/β-catenin signaling pathway by the CTNNB1 mutation.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α7 nicotinic acetylcholine receptor agonist attenuates allergen-induced immediate nasal response in murine model of allergic rhinitis. α7烟碱乙酰胆碱受体激动剂可减轻过敏原诱导的小鼠过敏性鼻炎模型的即时鼻腔反应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-06-04 DOI: 10.1292/jvms.24-0033

Shuhei Yamashita, Kento Miura, Anna Matsuura, Norimasa Yamasaki, Naoto Uda, Sawako Ogata, Naohisa Hosomi, Shotaro Nakajima, Noriko Kitamura, Minoru Gotoh, Akio Mori, Osamu Kaminuma

引用次数: 0

Efficacy and adverse events of L-Asparaginase administration as a first-line treatment for feline large-cell gastrointestinal lymphoma. L-天冬酰胺酶作为一线治疗猫大细胞胃肠道淋巴瘤的疗效和不良反应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-06-03 DOI: 10.1292/jvms.23-0453

Haruka Inazumi, Hiroto Toyoda, Shotaro Shimano, Hiroki Sakuma, Megumi Sakamoto, Taisuke Nakagawa, Ray Fukuoka, Aki Ohmi, James K Chambers, Kazuyuki Uchida, Yuko Goto-Koshino, Hirotaka Tomiyasu

{"title":"Efficacy and adverse events of L-Asparaginase administration as a first-line treatment for feline large-cell gastrointestinal lymphoma.","authors":"Haruka Inazumi, Hiroto Toyoda, Shotaro Shimano, Hiroki Sakuma, Megumi Sakamoto, Taisuke Nakagawa, Ray Fukuoka, Aki Ohmi, James K Chambers, Kazuyuki Uchida, Yuko Goto-Koshino, Hirotaka Tomiyasu","doi":"10.1292/jvms.23-0453","DOIUrl":"10.1292/jvms.23-0453","url":null,"abstract":"L-Asparaginase (L-Asp) is often used to induce remission in feline large-cell gastrointestinal lymphoma (LCGIL). However, no study has evaluated the efficacy and adverse events following the initial use of this drug as a first-line treatment in feline LCGIL. We retrospectively reviewed medical records of cats with LCGIL treated with L-Asp to induce remission. This study included 43 cats. The response rate (RR) after the first administration of L-Asp was 37.2% (Complete remission: 7.0%, partial remission: 30.2%). RR was significantly higher in cases with primary gastric lesions (64.3%) than in those with primary intestinal lesions (24.1%) (P=0.018), and it was also higher in cases without anemia (57.1%) than those with anemia (15.0%) (P=0.009). The most common adverse event was hyperammonemia, which occurred in 10 of 12 cases where we could compare plasma ammonia concentrations before and after the first dose of L-Asp. Plasma phosphate concentrations were also significantly increased (P<0.001) within 24 hr after the first dose. Decreased appetite, vomiting, and diarrhea were also observed in five, three, and seven cases, respectively, and Grade 3 or higher gastrointestinal signs were observed as adverse events in three cases. The median overall survival of all cats was 150 days (range, 5-1,065 days), and the median progression-free survival was 104 days (range, 2-978 days). In conclusion, L-Asp was effective to induce remission, and severe adverse events were uncommon in feline LCGIL.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the impact of application of anaerobic bacterial fermentation enhancer on digital dermatitis. 评估应用厌氧细菌发酵增强剂对数字皮炎的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-06-05 DOI: 10.1292/jvms.23-0442

Ryota Matsuyama, Noritsugu Abe, Ayano Sato, Yui Ishikura, Takaaki Ishikawa, Ayaka Otake, Ryunosuke Watanabe, Mitsutoshi Sunadome, Kohei Makita, Takashi Murakami

引用次数: 0

Reduction of phosphorylated signal transducer and activator of transcription-5 expression in feline mammary carcinoma. 降低猫乳腺癌中磷酸化信号转导和转录激活因子-5 的表达。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-21 DOI: 10.1292/jvms.23-0470

Keishi Owaki, Mami Murakami, Kana Kato, Akihiro Hirata, Hiroki Sakai

{"title":"Reduction of phosphorylated signal transducer and activator of transcription-5 expression in feline mammary carcinoma.","authors":"Keishi Owaki, Mami Murakami, Kana Kato, Akihiro Hirata, Hiroki Sakai","doi":"10.1292/jvms.23-0470","DOIUrl":"10.1292/jvms.23-0470","url":null,"abstract":"Signal transducers and activators of transcription (STATs) are a family of transcription factors involved in various normal physiological cellular processes. Moreover, STATs have been recently identified as novel therapeutic targets for various human tumors. STAT3, STAT5a, and STAT6 have been suggested to be involved in tumorigenesis in human breast cancer. Owing to the similarity between feline mammary carcinomas (FMCs) and human breast cancers, these factors may play an important role in FMCs. However, studies on the expression of STATs in animal tumors are limited. Therefore, in this study, we aimed to characterize the expression of total STAT5 (tSTAT5) and phosphorylated STAT5 (pSTAT5) in FMCs, feline mammary adenomas, non-neoplastic proliferative mammary gland lesions, and normal feline mammary glands using immunohistochemistry. High expression of tSTAT5 was observed in the cytoplasm of all the samples assessed in this study. Moreover, high expression of tSTAT5 was observed in the nucleus; however, its levels varied depending on the lesion. The percentage of pSTAT5-nuclear positive cells varied among normal feline mammary glands (40.1 ± 25.1%), and non-neoplastic lesions, including mammary hyperplasia (43.2 ± 28.6%) and fibroadenomatous changes (18.0 ± 13.6%). Moreover, the percentage of pSTAT5-nuclear-positive cells in feline mammary adenomas was 24.5 ± 19.2%, which was significantly reduced in feline mammary carcinomas (2.4 ± 5.6%), regardless of histopathological subtype. This study suggests that decreased STAT5 activity may be involved in the development and malignant progression of feline mammary carcinomas.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innate immune response of bovine mammary epithelial cells in Mycoplasma bovis mastitis using an in vitro model of bovine mammary gland infection. 利用牛乳腺感染的体外模型研究牛乳腺上皮细胞在牛支原体乳腺炎中的先天性免疫反应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-07 DOI: 10.1292/jvms.24-0097

Noriko Imaizumi, Satoshi Gondaira, Marin Kamioka, Tomochika Sugiura, Ayako Eguchi, Koji Nishi, Jumpei Fujiki, Hidetomo Iwano, Hidetoshi Higuchi

{"title":"Innate immune response of bovine mammary epithelial cells in Mycoplasma bovis mastitis using an in vitro model of bovine mammary gland infection.","authors":"Noriko Imaizumi, Satoshi Gondaira, Marin Kamioka, Tomochika Sugiura, Ayako Eguchi, Koji Nishi, Jumpei Fujiki, Hidetomo Iwano, Hidetoshi Higuchi","doi":"10.1292/jvms.24-0097","DOIUrl":"10.1292/jvms.24-0097","url":null,"abstract":"Mycoplasma bovis mastitisis highly contagious and disrupts lactation, posing a significant threat to the dairy industry. While the mammary gland's defence mechanism involves epithelial cells and mononuclear cells (MNC), their interaction with M. bovis remains incompletely understood. In this study, we assessed the immunological reactivity of bovine mammary epithelial cells (bMEC) to M. bovis through co-culture with MNC. Upon co-culture with MNC, the mRNA expression levels of interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α in bMEC stimulated by M. bovis showed a significant increase compared to monoculture. Additionally, when stimulated with M. bovis, the culture supernatant exhibited significantly higher concentrations of IL-6 and interferon (IFN)-γ, while IL-1β concentration tended to be higher in co-culture with MNC than in monoculture. Furthermore, the mRNA expression levels of toll-like receptor (TLR) 2 in bMEC stimulated with M. bovis tended to increase, and TLR4 significantly increased when co-cultured with MNC compared to monocultures. However, the surface expression levels in bMEC did not exhibit significant changes between co-culture and monoculture. Overall, our research indicates that the inflammatory response of bMEC is increased during co-culture with MNC, suggesting that the interaction between bMEC and MNC in the mammary gland amplifies the immune response to M. bovis in cows affected by M. bovis mastitis.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140862925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic analysis of bone marrow specimens collected from Miniature Dachshunds diagnosed with non-neoplastic bone marrow disorders. 对从被诊断患有非肿瘤性骨髓疾病的迷你腊肠犬身上采集的骨髓标本进行转录组分析。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-06-03 DOI: 10.1292/jvms.23-0371

Akiyoshi Tani, Kota Nakase, Hirotaka Tomiyasu, Sakurako Neo, Aki Ohmi, Yuko Goto-Koshino, Koichi Ohno, Hajime Tsujimoto

{"title":"Transcriptomic analysis of bone marrow specimens collected from Miniature Dachshunds diagnosed with non-neoplastic bone marrow disorders.","authors":"Akiyoshi Tani, Kota Nakase, Hirotaka Tomiyasu, Sakurako Neo, Aki Ohmi, Yuko Goto-Koshino, Koichi Ohno, Hajime Tsujimoto","doi":"10.1292/jvms.23-0371","DOIUrl":"10.1292/jvms.23-0371","url":null,"abstract":"Non-neoplastic bone marrow disorders are main causes of non-regenerative anemia in dogs. Despite the high incidence of the diseases, their molecular pathophysiology has not been elucidated. We previously reported that Miniature Dachshund (MD) was a predisposed breed to be diagnosed with non-neoplastic bone marrow disorders in Japan, and immunosuppressive treatment-resistant MDs showed higher number of platelets and morphological abnormalities in peripheral blood cells. These data implied that treatment-resistant MDs might possess distinct pathophysiological features from treatment-responsive MDs. Therefore, we conducted transcriptomic analysis of bone marrow specimens to investigate the pathophysiology of treatment-resistant MDs. Transcriptomic analysis comparing treatment-resistant MDs and healthy control dogs identified 179 differentially expressed genes (DEGs). Pathway analysis using these DEGs showed that \"Wnt signaling pathway\" was a significantly enriched pathway. We further examined the expression levels of DEGs associated with Wnt signaling pathway and confirmed the upregulation of AXIN2 and CCND2 and the downregulation of SFRP2 in treatment-resistant MDs compared with treatment-responsive MDs and healthy control dogs. This alteration implied the activation of Wnt signaling pathway in treatment-resistant MDs. The activation of Wnt signaling pathway has been reported in human patients with myelodysplastic syndrome (MDS), which is characterized by dysplastic features of blood cells. Therefore, the results of this study implied that treatment-resistant MDs have distinct molecular pathological features from treatment-responsive MDs and the pathophysiology of treatment-resistant MDs might be similar to that of human MDS patients.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of doubled dose administration of foot-and-mouth disease vaccine against heterologous virus infection in cattle. 加倍剂量注射口蹄疫疫苗对牛异源病毒感染的影响

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-09 DOI: 10.1292/jvms.24-0115

Rie Kawaguchi, Tatsuya Nishi, Katsuhiko Fukai, Mitsutaka Ikezawa, Takehiro Kokuho, Kazuki Morioka

{"title":"Effect of doubled dose administration of foot-and-mouth disease vaccine against heterologous virus infection in cattle.","authors":"Rie Kawaguchi, Tatsuya Nishi, Katsuhiko Fukai, Mitsutaka Ikezawa, Takehiro Kokuho, Kazuki Morioka","doi":"10.1292/jvms.24-0115","DOIUrl":"10.1292/jvms.24-0115","url":null,"abstract":"Vaccination is a feasible approach for controlling foot-and-mouth disease (FMD). In FMD-free countries, vaccines are stored as a precautionary measure to control potential outbreaks. However, the challenge lies in pre-stocking optimal vaccines against the newly emerging strains. This study examined the potency of pre-stocked vaccines administered at elevated doses during emergencies. We vaccinated the cows with either a single or double trivalent vaccine dose containing two serotype O and one serotype A strains. Subsequently, vaccinated and unvaccinated cows were exposed to virulent strains of serotype O (O/JPN/2010; topotype Southeast Asia/Mya-98 lineage) or A (A/IRN/2016; topotype ASIA/G-VII lineage), which were genetically and antigenically distinct from the vaccine strains. Following challenge infections, all cows that received a single dose vaccination exhibited vesicular lesions with excreted viruses in the oral and nasal discharges. However, a substantial reduction was observed in the total clinical scores and virus titers in the sera and nasal discharges compared to those in the unvaccinated group. Cows receiving a doubled dose vaccination were completely protected from infection with O/JPN/2010 or demonstrated a significant decrease in viral shedding and clinical scores against A/IRN/2016. To note, vesicular lesions harbor significant amounts of viruses; thus, by mitigating their formation, viral transmission can be impeded, thereby slowing viral spread in the field. Furthermore, increasing the vaccine dose induced higher neutralizing antibody titers against heterologous strains. These findings suggest an alternative strategy for the effective management of future epidemics using pre-stocked vaccines.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140892171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Appearance of small extracellular vesicles in the mouse pregnant serum and the localization in placentas. 小鼠妊娠血清中出现的小细胞外囊泡及其在胎盘中的定位。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-15 DOI: 10.1292/jvms.24-0047

Lita Rakhma Yustinasari, Muneyoshi Hyoto, Hiroyuki Imai, Ken Takeshi Kusakabe

{"title":"Appearance of small extracellular vesicles in the mouse pregnant serum and the localization in placentas.","authors":"Lita Rakhma Yustinasari, Muneyoshi Hyoto, Hiroyuki Imai, Ken Takeshi Kusakabe","doi":"10.1292/jvms.24-0047","DOIUrl":"10.1292/jvms.24-0047","url":null,"abstract":"Exosomes or small extracellular vesicles (sEVs) are present in the blood of pregnant mice and considered to be involved in pregnancy physiology. Although sEVs in pregnant periods are proposed to be derived from placentas, sEVs-producing cells are not well known in mouse placentas. We studied the dynamics and localization of sEVs in pregnant serum and placentas, and examined gestational variation of microRNA (miRNA). Serums and placentas were collected from non-pregnant (NP) and pregnant mice throughout the entire gestational day (Gd). EVs were purified from serums and total RNA was isolated from EVs. Nanoparticle-tracking assay (NTA) revealed that the rates of sEVs in EVs are 53% at NP, and increased to 80.1% at Gd 14.5 and 97.5% at Gd 18.5. Western blotting on EVs showed positive reactivity to the tetraspanin markers and clarified that the results using anti-CD63 antibody were most consistent with the sEVs appearance detected by NTA. Serum EVs also showed a positive reaction to the syncytiotrophoblast marker, syncytin-1. Immunohistostaining using anti-CD63 antibody showed positive reactions in mouse placentas at the syncytiotrophoblasts and endothelial cells of the fetal capillaries. Quantitative PCR revealed that significantly higher amounts of miRNAs were included in the sEVs of Gd 18.5. Our results suggested that sEVs are produced in the mouse placenta and transferred to maternal or fetal bloodstreams. sEVs are expected to have a miRNA-mediated physiological effect and become useful biomarkers reflecting the pregnancy status.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0