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Detection of novel coltivirus-related sequences in Haemaphysalis megaspinosa ticks collected from Kanagawa Prefecture, Japan. 在日本神奈川县采集的 Haemaphysalis megaspinosa 蜱虫中检测到新型大肠杆菌相关序列。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-14 DOI: 10.1292/jvms.24-0124
Ryo Matsumura, Daisuke Kobayashi, Kyo Itoyama, Haruhiko Isawa
{"title":"Detection of novel coltivirus-related sequences in Haemaphysalis megaspinosa ticks collected from Kanagawa Prefecture, Japan.","authors":"Ryo Matsumura, Daisuke Kobayashi, Kyo Itoyama, Haruhiko Isawa","doi":"10.1292/jvms.24-0124","DOIUrl":"10.1292/jvms.24-0124","url":null,"abstract":"<p><p>Coltiviruses, belonging to the genus Coltivirus within the family Spinareoviridae, are predominantly tick-borne viruses. Some of these species have been implicated in human diseases; however, their diversity, geographical distribution, and evolutionary dynamics remain inadequately. Therefore, this study was undertaken to explore the phylogenetic evolution of coltiviruses and related viruses. Our results revealed the detection of novel coltivirus-related sequences in adult female Haemaphysalis megaspinosa ticks collected from Kanagawa Prefecture, Japan. Molecular phylogenetic analysis revealed a close association between the sequences and the genome sequences of known coltivirus-related viruses, namely Qinghe tick reovirus and Fennes virus. The putative coltivirus-related virus was tentatively designated the Nakatsu tick virus. This study provides insights into the phylogenetic evolution of coltiviruses and related viruses.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimation of latamoxef (moxalactam) dosage regimens against β-lactamase-producing Enterobacterales in dogs: a pharmacokinetic and pharmacodynamic analysis using Monte Carlo simulation. 针对狗体内产β-内酰胺酶肠杆菌的拉他莫昔（莫沙内酰胺）剂量方案估算：利用蒙特卡罗模拟进行的药代动力学和药效学分析。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-18 DOI: 10.1292/jvms.24-0197
Mizuki Kusumoto, Haruka Narita, Tomoki Motegi, Kazuki Harada
{"title":"Estimation of latamoxef (moxalactam) dosage regimens against β-lactamase-producing Enterobacterales in dogs: a pharmacokinetic and pharmacodynamic analysis using Monte Carlo simulation.","authors":"Mizuki Kusumoto, Haruka Narita, Tomoki Motegi, Kazuki Harada","doi":"10.1292/jvms.24-0197","DOIUrl":"10.1292/jvms.24-0197","url":null,"abstract":"<p><p>One of the most significant research areas in veterinary medicine is the search for carbapenem substitutes for the treatment of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales (ESBL-E). This study applied a pharmacokinetic/pharmacodynamic (PK/PD) strategy in validating optimal latamoxef (LMX) therapeutic regimens against canine ESBL-E infections. Five dogs were administered a bolus dose of 40 mg/kg LMX intravenously to measure serum drug concentrations and determine PK indices using the noncompartmental model. The highest minimum inhibitory concentration (MIC) with a probability of target attainment ≥90% was used to compute the PK/PD cutoff values for bacteriostatic (time for which the unbound drug concentration was above the MIC [fTAM] ≥ 40%) and bactericidal (fTAM ≥ 70%) effects when administered at 20, 30, 50, and 60 mg/kg, in addition to 40 mg/kg. The cumulative fraction of response (CFR) was determined using the MIC distribution of wild-type ESBL-E in companion animals. The PK/PD cutoff values can be increased by reducing the dosing interval rather than increasing the dose per time. Based on the calculated CFRs for ESBL-producing Escherichia coli and Klebsiella pneumoniae, all LMX regimens in this study and those administered at 30-60 mg/kg every 8 and 6 hr were found to be optimal (CFR ≥ 90%) for exerting bacteriostatic and bactericidal effects, respectively. However, the regimens of 50 and 60 mg/kg every 6 hr may merely exert bacteriostatic effects on ESBL-producing Enterobacter cloacae. Further clinical trials are required to confirm the clinical efficacy of LMX.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141428017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of collagen matrix (DuraGen®) to reduce subcutaneous emphysema in canine dorsal rhinotomy. 应用胶原蛋白基质（DuraGen®）减少犬鼻背切口的皮下气肿。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-27 DOI: 10.1292/jvms.24-0141
Yongsun Kim, Byung-Jae Kang
{"title":"Application of collagen matrix (DuraGen<sup>®</sup>) to reduce subcutaneous emphysema in canine dorsal rhinotomy.","authors":"Yongsun Kim, Byung-Jae Kang","doi":"10.1292/jvms.24-0141","DOIUrl":"10.1292/jvms.24-0141","url":null,"abstract":"<p><p>This retrospective case study aimed to evaluate the efficacy of collagen matrix (DuraGen<sup>®</sup>) in preventing subcutaneous emphysema, a common complication following dorsal rhinotomy. Six client-owned dogs diagnosed with nasal masses using computed tomography were included in this study. Dorsal rhinotomy was performed, and a collagen matrix was used to seal bone defects before fixation of the nasal bone flap. Following collagen matrix application, all dogs recovered without notable complications. These findings suggest that the collagen matrix is a reliable and safe intervention for mitigating subcutaneous emphysema after dorsal rhinotomy.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forelimb lameness caused by malignant peripheral nerve sheath tumors of the median nerve in a dog: a case report. 犬正中神经恶性周围神经鞘瘤导致的前肢跛行：病例报告。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-07-01 DOI: 10.1292/jvms.24-0038
Noriyuki Miyaho, Manabu Mochizuki, Muneki Honnami
{"title":"Forelimb lameness caused by malignant peripheral nerve sheath tumors of the median nerve in a dog: a case report.","authors":"Noriyuki Miyaho, Manabu Mochizuki, Muneki Honnami","doi":"10.1292/jvms.24-0038","DOIUrl":"10.1292/jvms.24-0038","url":null,"abstract":"<p><p>An 8-year-old Portuguese Water Dog presented with a 5-month history of left forelimb lameness. There was palmar pain. Ultrasonography revealed enlargement of the left median nerve. Subsequent MRI also showed enlargement of the median nerve in the distal palmar to the mid-forearm region. Rapid intraoperative diagnosis suggested malignant peripheral nerve sheath tumors (MPNST) and a neurotomy was performed. The lameness had almost disappeared in 1 month after surgery. Recurrence occurred 26 months postoperatively and the forelimb was amputated. At 950 days after the neurotomy, radiography revealed lung metastasis, and the dog died 988 days after the neurotomy. Neurotomy for MPNST should be performed with caution until more information is available regarding methods for early detection, margin determination, and indication determination for neurotomy.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141472052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perioperative dobutamine infusion in an African lion (Panthera leo). 非洲狮（Panthera leo）围手术期输注多巴酚丁胺。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-25 DOI: 10.1292/jvms.23-0436
Donghwi Shin, Yoocheol Jeong, Seongchan Yeon, Inhyung Lee, Won-Gyun Son
{"title":"Perioperative dobutamine infusion in an African lion (Panthera leo).","authors":"Donghwi Shin, Yoocheol Jeong, Seongchan Yeon, Inhyung Lee, Won-Gyun Son","doi":"10.1292/jvms.23-0436","DOIUrl":"10.1292/jvms.23-0436","url":null,"abstract":"<p><p>Laparoscopic ovariectomy under general anesthesia was planned in a 10-year-old, 146 kg, apparently healthy female African lion (Panthera leo). The lion was immobilized via intramuscular darts containing midazolam (0.033 mg/kg), medetomidine (50 µg/kg) and ketamine (2.5 mg/kg), and intubated using an endotracheal tube (16 mm internal diameter). The anesthesia was maintained using sevoflurane (0.9-2.1% end-tidal concentration), in combination with remifentanil (0.1 µg/kg/min) and ketamine (11 µg/kg/min) at a constant rate infusion (CRI), with Hartmann's solution (5 mL/kg/hr). Surgery was conducted with stable vital signs, but hypotension (mean arterial blood pressure 55 mmHg) developed, requiring dobutamine treatment. The hypotension was effectively controlled by adjusting dobutamine from 5 µg/kg/min to 0.2 to 0.3 µg/kg/min. This case suggests possibilities that dosages in this range can be clinically useful for peri-anesthetic hypotension in lions.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolated left hypoplasia of the pulmonary artery accompanied by lung hypoplasia and chronic bronchial disease in a cat. 一只猫的左肺动脉孤立性发育不全，并伴有肺发育不全和慢性支气管疾病。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-14 DOI: 10.1292/jvms.23-0451
Mihyun Choi, Namsoon Lee
{"title":"Isolated left hypoplasia of the pulmonary artery accompanied by lung hypoplasia and chronic bronchial disease in a cat.","authors":"Mihyun Choi, Namsoon Lee","doi":"10.1292/jvms.23-0451","DOIUrl":"10.1292/jvms.23-0451","url":null,"abstract":"<p><p>A 2-year-old castrated Russian Blue cat presented with inappetence, depression, and labored respirations. Radiography findings suggested left lung atelectasis; however, the ultrasonography findings did not indicate lung atelectasis. The left pulmonary artery (PA) was abnormally small on echocardiography; further, there were no other cardiac anomalies. Computed tomography revealed an abnormally small left PA and left lung. Furthermore, bronchiectasis and tree-in-bud patterns were observed in the lung lobes. Based on these findings, the cat was diagnosed with isolated left PA hypoplasia, presumed left lung hypoplasia, and feline chronic bronchial disease. Early diagnosis of this disease is important because it can cause serious complications, including recurrent respiratory infection, bronchiectasis, massive hemoptysis, and pulmonary hypertension.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of a simple method for determining the time of insemination and different methods on artificial insemination in common bottlenose dolphins (Tursiops truncatus). 确定授精时间的简单方法和不同方法对普通瓶鼻海豚人工授精的影响。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-06-27 DOI: 10.1292/jvms.23-0523
Keiko Yamamoto, Nobuyuki Kashiwagi, Mika Otsuka, Tatsuya Hori
{"title":"Effects of a simple method for determining the time of insemination and different methods on artificial insemination in common bottlenose dolphins (Tursiops truncatus).","authors":"Keiko Yamamoto, Nobuyuki Kashiwagi, Mika Otsuka, Tatsuya Hori","doi":"10.1292/jvms.23-0523","DOIUrl":"10.1292/jvms.23-0523","url":null,"abstract":"<p><p>In this study, we examined the usefulness of a simpler and more feasible method for determining the optimal timing of artificial insemination and the conditions for its success in six female common bottlenose dolphins. Pregnancy was successfully achieved in five dolphins by performing intrauterine insemination, using chilled semen stored for less than 3 days or frozen semen within 24 hr of exhibiting a peak serum estradiol (E<sub>2</sub>) level of 100 pg/mL or higher or on the day with a serum E<sub>2</sub> level of approximately 100 pg/mL, measured with a simple measuring device. We concluded that the determining the optimal timing of intrauterine insemination by measuring serum E<sub>2</sub> levels is a simpler and more useful method compared with the conventional approach.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141460394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A case of olfactory ganglioneuroblastoma in a dog: immunohistochemical comparison with olfactory neuroepithelia and olfactory neuroblastomas. 一例狗嗅神经节神经母细胞瘤：与嗅神经上皮细胞和嗅神经母细胞瘤的免疫组化比较。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-08-02 Epub Date: 2024-07-02 DOI: 10.1292/jvms.24-0181
Kio Yoshida, James K Chambers, Kei Ito, Hidehiro Hirao, Kazumi Nibe, Kazuyuki Uchida
{"title":"A case of olfactory ganglioneuroblastoma in a dog: immunohistochemical comparison with olfactory neuroepithelia and olfactory neuroblastomas.","authors":"Kio Yoshida, James K Chambers, Kei Ito, Hidehiro Hirao, Kazumi Nibe, Kazuyuki Uchida","doi":"10.1292/jvms.24-0181","DOIUrl":"10.1292/jvms.24-0181","url":null,"abstract":"<p><p>In the present study, histopathological and immunohistochemical findings of olfactory ganglioneuroblastoma in a dog were compared to those of canine olfactory neuroepithelia and neuroblastomas. Olfactory ganglioneuroblastoma consists of ganglion cell-like tumor cells with Schwannian stroma and neuroblast-like tumor cells. Immunohistochemically, ganglion cell-like tumor cells were immunopositive for synaptophysin, β3-tubulin, and tyrosine hydroxylase, Schwannian stroma was immunopositive for GFAP and SOX2, and neuroblast-like tumor cells were immunopositive for OLIG2, β3-tubulin, SOX2, cytokeratin AE1/AE3, and p63. The immunohistochemical results of olfactory neuroepithelia and olfactory neuroblastomas were similar to those of neuroblast-like tumor cells. These results suggest that the ganglion cell-like tumor cells in the present case have a sympathetic neuron immunophenotype, whereas neuroblast-like tumor cells have an olfactory neuroepithelial immunophenotype.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11300123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characteristics of failure of passive transfer at the herd level using the serum immunoglobulin G concentration as an indicator on dairy farms in eastern Hokkaido, Japan. 日本北海道东部奶牛场以血清免疫球蛋白 G 浓度为指标的牛群被动转群失败特征。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-31 DOI: 10.1292/jvms.24-0054
Shuji Kayasaki, Hitomi Satoh, Keitaro Oguchi, Kyoko Chisato, Rika Fukumori, Hidetoshi Higuchi, Kazuyuki Suzuki, Shin Oikawa
{"title":"Characteristics of failure of passive transfer at the herd level using the serum immunoglobulin G concentration as an indicator on dairy farms in eastern Hokkaido, Japan.","authors":"Shuji Kayasaki, Hitomi Satoh, Keitaro Oguchi, Kyoko Chisato, Rika Fukumori, Hidetoshi Higuchi, Kazuyuki Suzuki, Shin Oikawa","doi":"10.1292/jvms.24-0054","DOIUrl":"10.1292/jvms.24-0054","url":null,"abstract":"<p><p>The objectives of this study were to conduct a survey of failure-of-passive-transfer (FPT) in eastern Hokkaido Japan, to evaluate the association between herd-level FPT and death and culling or treatment, and to test the effectiveness of monitoring using herd-level FPT. A total of 4,411 Holstein and Holstein-Wagyu crossbreds calves born from Holstein dams during the year beginning April 2, 2019 on 39 dairy farms were included in the study to investigate death-and-culling and the treatment rate during the first month of life, as well as rearing management up to 3 weeks of age. A subset of Holsteins (n=381) was included in the study for passive transfer and farms were diagnosed as having FPT if more than 20% of newborn calves had serum IgG levels below 10 g/L at the herd level. The prevalence of FPT (<IgG 10 g/L) on farms was significantly correlated (r=0.27, P<0.05) with the death-and-culling rate. Binomial logistic regression analysis showed that FPT farms had a significantly higher risk of being high death-and-culling farms than non-FPT farms (odds ratio: 5.20, P<0.05), emphasizing the importance of colostrum feeding. Farms not using frozen stored colostrum had a significantly higher risk of being FPT farms than those that did (odds ratio: 4.13, P<0.05), emphasizing the importance of feeding colostrum from the dam. Monitoring herd-level FPT was useful in assessing whether the problem of calf death and culling lies in passive transfer.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stable expression of red fluorescent protein-blasticidin deaminase fusion gene (rfp-bsd) as a selectable marker for DNA transfection in Babesia ovata. 稳定表达红色荧光蛋白-木质素脱氨酶融合基因（rfp-bsd），作为卵形巴贝虫 DNA 转染的选择性标记。
IF 1.1 4区 农林科学
Journal of Veterinary Medical Science Pub Date : 2024-07-02 Epub Date: 2024-05-15 DOI: 10.1292/jvms.24-0111
Nada Arayaskul, Masahito Asada, Atefeh Fathi, Nanang R Ariefta, Kota Komatsu, Keisuke Suganuma, Noboru Inoue, Shin-Ichiro Kawazu
{"title":"Stable expression of red fluorescent protein-blasticidin deaminase fusion gene (rfp-bsd) as a selectable marker for DNA transfection in Babesia ovata.","authors":"Nada Arayaskul, Masahito Asada, Atefeh Fathi, Nanang R Ariefta, Kota Komatsu, Keisuke Suganuma, Noboru Inoue, Shin-Ichiro Kawazu","doi":"10.1292/jvms.24-0111","DOIUrl":"10.1292/jvms.24-0111","url":null,"abstract":"<p><p>The red fluorescent protein (rfp)-blasticidin deaminase (bsd) fusion gene was transfected into Babesia ovata by electroporation with the plasmid DNA and selected with 15 μg/mL of blasticidin S under the in vitro culture condition. The transfected parasite with episomal DNA was selected and cultured for further analysis based on the presence of the rfp-bsd fusion gene by PCR and expression of the fusion protein by immunofluorescence antibody test under fluorescence microscopy for 2 months after the transfection. The results are the first, to our knowledge, to demonstrate the expression and stability of the episomal rfp-bsd fusion gene under the control of actin promoter as a selectable marker for the transfection system in B. ovata.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11251815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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