Journal of Veterinary Medical Science最新文献_第8页

The Na⁺/Ca²⁺ exchanger: molecular structure, functional regulation, and mode-selective modulation. Na+/Ca2+交换器：分子结构，功能调节和模式选择调制。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-10 DOI: 10.1292/jvms.26-0186

Naoshige Ono, Kazuhiro Nishiyama, Yasu-Taka Azuma

{"title":"The Na+/Ca2+ exchanger: molecular structure, functional regulation, and mode-selective modulation.","authors":"Naoshige Ono, Kazuhiro Nishiyama, Yasu-Taka Azuma","doi":"10.1292/jvms.26-0186","DOIUrl":"10.1292/jvms.26-0186","url":null,"abstract":"The Na+/Ca2+ exchanger (NCX) is a key regulator of intracellular Ca2+ homeostasis, mediating bidirectional ion transport in response to electrochemical gradients. NCX plays essential roles in diverse physiological processes, including cardiac contraction, neuronal signaling, and hormone secretion, while its dysregulation is implicated in a wide range of pathophysiological conditions such as heart failure, ischemia, and neurodegenerative diseases. Recent advances in structural biology, particularly cryo-electron microscopy, have provided detailed insights into the molecular architecture of eukaryotic NCX, revealing the mechanisms underlying ion transport, regulation, and inactivation. These studies have highlighted the functional importance of conserved structural elements, including the α1- and α2-repeats and cytosolic regulatory domains, in governing exchanger activity. In parallel, pharmacological studies have identified a variety of NCX modulators with distinct mode and isoform selectivity. While classical inhibitors predominantly target the reverse mode, emerging compounds-including highly selective inhibitors and novel activators-demonstrate diverse mechanisms of action, often involving allosteric modulation rather than direct interaction with the ion-binding site. This review integrates current knowledge of NCX structure, function, and pharmacology, with a particular focus on the relationship between structural determinants and transport mode selectivity. We also discuss the challenges that have limited the clinical translation of NCX-targeting drugs and outline future perspectives for the rational design of next-generation NCX modulators.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1407-1418"},"PeriodicalIF":1.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148431424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial species distribution and antimicrobial susceptibility of canine ocular isolates in Japan: retrospective analysis of a diagnostic laboratory database. 日本犬眼部分离株的细菌种类分布和抗菌药物敏感性：诊断实验室数据库的回顾性分析。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-13 DOI: 10.1292/jvms.26-0297

Mei Sakamoto, Yukihiro Oyama, Chikara Satake, Kazuki Harada

引用次数: 0

Testicular development, sexual maturation, and seasonal variation in spermatogenesis of Raccoons in Wakayama Prefecture. 和歌山县浣熊睾丸发育、性成熟和精子发生的季节变化。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-07 DOI: 10.1292/jvms.26-0163

Makoto Asano, Yukiko Kato, Kazuo Suzuki

引用次数: 0

Commercial transport of spent layer breeder chickens: process and strain-specific physiological and behavioral responses. 废蛋鸡的商业运输：过程和品系特异性生理和行为反应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-06 DOI: 10.1292/jvms.25-0598

Yuko Moroki, Shoji Fukamachi

{"title":"Commercial transport of spent layer breeder chickens: process and strain-specific physiological and behavioral responses.","authors":"Yuko Moroki, Shoji Fukamachi","doi":"10.1292/jvms.25-0598","DOIUrl":"10.1292/jvms.25-0598","url":null,"abstract":"Understanding the underlying issues related to animal transport is essential for promoting animal welfare. However, no research has focused on the transport process and its physiological effects on spent layer breeder chickens. Therefore, this study aimed to investigate a single commercial transport process in spent layer breeder chickens and evaluated its physiological and behavioral effects across three strains of focal hens: Sakura, Lohmann Julia (Julia), and Hy-Line Brown (Brown). A total of 1,611 chickens (1,547 hens and 64 roosters, aged 700 days) including various crossbreeds and lineages were transported over 32.9 km in 55 min. The total mortality rate was 0.25%. Each chicken was sequentially housed in two transport crate types and remained there until shackling. The maximum duration from initial capture to shackling was 7 hr 30 min. Temperatures inside cages and crates were higher than ambient conditions. Transported focal hens showed reduced body weight and elevated heterophil-to-lymphocyte ratios. Brown hens exhibited higher heterophil-to-lymphocyte ratios and lower core body temperatures compared to the overall mean. Additionally, transported focal hens exhibited wet plumage, shivering, and piloerection due to rain exposure, although temperatures near them remained within their thermal comfort zone. These findings reveal the complexity of the transport process and its adverse effects on spent layer breeder chickens, highlighting genetic differences among strains in physiological and behavioral responses. They also underscore the need for careful animal monitoring, preventing wetting, and adopting strain-specific transport management practices to improve animal welfare.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1318-1328"},"PeriodicalIF":1.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148392261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological survey of bovine leukemia virus in beef cattle in the Tohoku region of Japan. 日本东北地区肉牛白血病病毒流行病学调查。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-13 DOI: 10.1292/jvms.26-0151

Takenori Arai, Sonoko Kondo, Mayumi Shiobara, Saki Uehara, Kei-Ichi Matsuda, Kazunori Suzuki, Toshihiro Ichijo, Takehisa Yamamoto, Kaoru Hatate, Shinji Yamada, Kenji Murakami

{"title":"Epidemiological survey of bovine leukemia virus in beef cattle in the Tohoku region of Japan.","authors":"Takenori Arai, Sonoko Kondo, Mayumi Shiobara, Saki Uehara, Kei-Ichi Matsuda, Kazunori Suzuki, Toshihiro Ichijo, Takehisa Yamamoto, Kaoru Hatate, Shinji Yamada, Kenji Murakami","doi":"10.1292/jvms.26-0151","DOIUrl":"10.1292/jvms.26-0151","url":null,"abstract":"The incidence of enzootic bovine leukosis, which is due to bovine leukemia virus (BLV), has been increasing in Japan. However, large-scale surveys have not been conducted in recent years, and the impact of cattle harboring high BLV proviral loads on within-farm BLV prevalence remains unclear. To clarify the situation, questionnaires and PCR and serological testing for BLV were conducted between 2022 and 2024 on 95 beef breeding farms (3,082 cattle) and 9 Japanese Black fattening farms (140 cattle) in the Tohoku region. We also examined the effects of various factors on within-farm proportions of BLV-positive cattle. The proportions of PCR-positive and seropositive cattle were 34.3% and 35.9% in beef breeding cattle and 15.7% and 15.0% in Japanese Black fattening cattle, respectively. Among the 74 farms with at least one PCR-positive animal, the proportion of PCR-positive cattle was significantly higher on farms with at least one animal with a high proviral load (≥400 copies/10 ng DNA) than on farms without any such cattle. Similarly, farms where high-proviral-load cattle accounted for≥10% of the herd had significantly higher proportions of PCR-positive cattle. In addition, farms that used public pastures, practiced on-farm grazing, or had experienced enzootic bovine leukosis within the past 5 years had significantly higher proportions of PCR-positive cattle. These findings suggest that BLV prevalence in beef cattle in the Tohoku region remains comparable to that reported approximately 10 years ago, indicating that BLV transmission in this region may be under control. Furthermore, cattle with high proviral loads may adversely affect within-farm BLV prevalence, indicating that targeted control measures focusing on such animals are essential for effective BLV control.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1377-1386"},"PeriodicalIF":1.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148431469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper analysis of laparoscopic liver biopsy tissue samples from cats. 猫的腹腔镜肝活检组织样本的铜分析。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-07 DOI: 10.1292/jvms.26-0074

Kaname Suzuki, Yumi Sakamoto, Rintaro Koshida, Hitoshi Shiozawa, Mizuho Shima, Yumiko Kagawa, Manabu Sakai

{"title":"Copper analysis of laparoscopic liver biopsy tissue samples from cats.","authors":"Kaname Suzuki, Yumi Sakamoto, Rintaro Koshida, Hitoshi Shiozawa, Mizuho Shima, Yumiko Kagawa, Manabu Sakai","doi":"10.1292/jvms.26-0074","DOIUrl":"10.1292/jvms.26-0074","url":null,"abstract":"Hepatic copper accumulation is assessed qualitatively via histopathology, and hepatic copper concentration is measured quantitatively. Laparoscopic biopsy provides samples adequate for histopathology; however, its suitability for copper quantification in cats remains unclear. Liver tissue samples from 19 cats undergoing laparoscopic liver biopsy were evaluated for hepatic copper score and concentration. Copper scores were determined by a board-certified pathologist using rhodanine-stained sections. Hepatic copper concentrations were determined using atomic absorption spectrometry, and the rhodanine-positive pixel ratio was calculated from histological images using ImageJ. Copper-associated hepatopathy was identified in three cats: two with a score of 4 and one with a score of 5. Seven cats demonstrated cholangiohepatitis (scores 0-3). The hepatic copper score was significantly correlated with copper concentration in the 19 cats (Spearman's r=0.757, P<0.001). Rhodanine-positive pixel ratio also correlated with copper concentration (Spearman's r=0.668, P<0.01). Therefore, laparoscopic biopsy provides feline liver tissue feasible for qualitative and quantitative copper analysis. The liver rhodanine-positive pixel ratio offers a useful quantitative alternative when hepatic copper concentration cannot be measured in cats.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1307-1313"},"PeriodicalIF":1.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148413496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arterial supply to the adrenal gland in the house musk shrew (Suncus murinus). 麝香鼩肾上腺的动脉供应。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-06-22 DOI: 10.1292/jvms.26-0209

Tetsuhito Kigata, Nonoha Miyai, Yuuki Horii, Takahiko Shiina, Yasutake Shimizu

引用次数: 0

Determining the dynamics of maternally derived antibodies in piglets prior to vaccination against porcine circovirus type 2. 确定仔猪接种PCV2疫苗前母源性抗体的动态。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-06 DOI: 10.1292/jvms.26-0120

Makoto Ukita, Yukine Kano, Saya Taharaguchi, Tadashi Takino, Kohei Makita, Katsuro Hagiwara

引用次数: 0

Long-term storage stability of multiplex immunochromatographic assay strips for serologic monitoring of infectious diseases in laboratory rodents. 用于实验室啮齿动物血清学监测的多重免疫层析试验条的长期储存稳定性。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-07-16 DOI: 10.1292/jvms.26-0161

Noriko Tosa, Tomoko Ishida, Kumiko Yoshimatsu, Nobuhito Hayashimoto, Akira Takakura, Jiro Arikawa

{"title":"Long-term storage stability of multiplex immunochromatographic assay strips for serologic monitoring of infectious diseases in laboratory rodents.","authors":"Noriko Tosa, Tomoko Ishida, Kumiko Yoshimatsu, Nobuhito Hayashimoto, Akira Takakura, Jiro Arikawa","doi":"10.1292/jvms.26-0161","DOIUrl":"10.1292/jvms.26-0161","url":null,"abstract":"Serological monitoring of infectious diseases in laboratory rodents is vital for ensuring animal health. Therefore, we have previously developed a multiplex immunochromatographic assay (ICA) to detect antibodies against six pathogens as follows: mouse hepatitis virus, Sendai virus, and Clostridium piliforme (Tyzzer's disease) in mice, and Seoul type orthohantavirus, Sendai virus, and sialodacryoadenitis virus in rats. However, the preparation of these strips is time-consuming and labor-intensive. Therefore, this study aimed to determine the long-term storage conditions for the developed multiplex ICA strips. The multiplex ICA strips stored in the polyethylene zipper-sealed bags (poly_bags) at -30°C maintained detectable reactivity for all six targeted pathogens for 36 months, regardless of the presence or absence of desiccants, oxygen absorbers, or a vacuum state. However, when stored at 18-28°C (room temperature) and 4°C, the detectable reactivity of the multiplex ICA strips stored in poly_bags was reduced for some of the six targets pathogens at 18 months after storage. This reduction was not prevented by the storage with the desiccants and in the vacuum-sealed poly_bags (vacuum_bags). The oxygen absorber reduced detectability of the multiplex ICA strips. These results indicate that storing ICA strips in poly_bags at -30°C, which is a simple method, is able to maintain detectability long-term for the strips format and pathogen targets examined in this study.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"1391-1398"},"PeriodicalIF":1.1,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rostral direction virtual laryngoscopy in canine glottis under sedation with butorphanol, medetomidine, and alfaxalone. 布托啡诺、美托咪定和阿法索龙镇静下犬声门的吻侧虚拟喉镜检查。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-01 Epub Date: 2026-06-23 DOI: 10.1292/jvms.26-0038

Kenji Teshima, Teppei Fujimoto, Naoko Shiozawa, Chieko Ishikawa, Yoshiki Yamaya

引用次数: 0