{"title":"Participation of autophagy in D-galactosamine-induced acute rat liver injury.","authors":"Nahid Rahman, Takeshi Izawa, Mitsuru Kuwamura, Jyoji Yamate","doi":"10.1292/jvms.25-0131","DOIUrl":null,"url":null,"abstract":"<p><p>Autophagy is an intracellular degradation system that generates autophagosomes. Autophagy was investigated in rat hepatic lesions induced by a single intraperitoneal injection of D-galactosamine (D-GalN) that can induce hepatic lesions mimicking human viral hepatitis. Lesions consisting of hepatocyte necrosis were sporadically seen within hepatic lobules on post-injection days 1 and 2, and recovered on days 3 and 5. The number of LC3B-immunopositive cytoplasmic granules (autophagosomes) increased significantly in hepatocytes on days 1 and 2 with increased Toll-like Receptor-2 (TLR-2) mRNA. Interestingly, there were some abnormal autophagosomes with vacuolated or irregular structures, being related to decreased mRNA level of Lamp2 at 8 hr and on days 2-5. These results indicated that autophagy may participate in the development of D-GalN-induced rat acute liver lesions through its activation or degradation.</p>","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"881-886"},"PeriodicalIF":1.1000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344380/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medical Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1292/jvms.25-0131","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/12 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Autophagy is an intracellular degradation system that generates autophagosomes. Autophagy was investigated in rat hepatic lesions induced by a single intraperitoneal injection of D-galactosamine (D-GalN) that can induce hepatic lesions mimicking human viral hepatitis. Lesions consisting of hepatocyte necrosis were sporadically seen within hepatic lobules on post-injection days 1 and 2, and recovered on days 3 and 5. The number of LC3B-immunopositive cytoplasmic granules (autophagosomes) increased significantly in hepatocytes on days 1 and 2 with increased Toll-like Receptor-2 (TLR-2) mRNA. Interestingly, there were some abnormal autophagosomes with vacuolated or irregular structures, being related to decreased mRNA level of Lamp2 at 8 hr and on days 2-5. These results indicated that autophagy may participate in the development of D-GalN-induced rat acute liver lesions through its activation or degradation.
期刊介绍：
JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
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