Journal of Veterinary Medical Science最新文献_第5页

Understanding knowledge, attitudes, and practices regarding antimicrobial use and compliance with prescriptions among pig farmers in Japan. 了解日本养猪户关于抗菌剂使用和处方依从性的知识、态度和做法。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-10-01 DOI: 10.1292/jvms.26-0168

Sayoko Hanamoto, Takeshi Sato, Yuko Endo, Kyoko Fujimoto, Eri Uchida-Fujii, Takeshi Haga, Katsuaki Sugiura

{"title":"Understanding knowledge, attitudes, and practices regarding antimicrobial use and compliance with prescriptions among pig farmers in Japan.","authors":"Sayoko Hanamoto, Takeshi Sato, Yuko Endo, Kyoko Fujimoto, Eri Uchida-Fujii, Takeshi Haga, Katsuaki Sugiura","doi":"10.1292/jvms.26-0168","DOIUrl":"https://doi.org/10.1292/jvms.26-0168","url":null,"abstract":"Inappropriate or unnecessary use of antimicrobials accelerates the emergence and dissemination of antimicrobial resistance. Veterinary prescriptions are a key mechanism for providing guidance regarding the appropriate use of antimicrobials from veterinarians to farmers. Because swine sectors consume the largest amount of veterinary antimicrobials in Japan, ensuring prudent use is an urgent priority. Therefore, this study aimed to evaluate pig farmers' compliance with prescriptions and identify their decision-making processes related to prescribed antimicrobial use to explore effective interventions for improving this compliance. We conducted a cross-sectional knowledge, attitudes, and practices (KAP) study among 1,314 pig farmers between December 2023 and February 2024 to evaluate how practices regarding the appropriateness of antimicrobial use and compliance with prescriptions were affected by their knowledge and attitudes. A total of 266 (20.2%) responses were included in our analysis. Although 97.7% of the farmers always complied with the withdrawal period in practice, 6.8% consistently self-adjusted the dose or frequency for their pigs instead of complying with prescriptions. Additionally, 3.0% consistently used antimicrobials for indications not specified in the prescriptions. Structural equation modeling yielded acceptable fit and indicated statistically significant paths from 'Relationship with the veterinarian' to 'Knowledge' (coefficient = 0.309, P<0.001) and 'Relationship with the veterinarian' to 'Practice' (0.425, P<0.001). This study revealed that not all pig farmers complied with all veterinary instructions. Strengthening the relationship between veterinarians and farmers is considered an effective strategy to improve prescription compliance.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149117680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chromosome 17 locus is involved in dorsal pigmentation in hooded Long-Evans Agouti rats. 染色体17位点参与了长-埃文斯大鼠背侧色素沉着。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-25 DOI: 10.1292/jvms.26-0391

Keisuke Sato, Teppei Nakamura, Naho Kotani, Takumi Yamamoto, Takatoshi Kaiura, Takashi Agui, Masami Morimatsu

{"title":"A chromosome 17 locus is involved in dorsal pigmentation in hooded Long-Evans Agouti rats.","authors":"Keisuke Sato, Teppei Nakamura, Naho Kotani, Takumi Yamamoto, Takatoshi Kaiura, Takashi Agui, Masami Morimatsu","doi":"10.1292/jvms.26-0391","DOIUrl":"https://doi.org/10.1292/jvms.26-0391","url":null,"abstract":"The hooded coat pattern in laboratory rats is produced by recessive alleles of the h (Kit) locus on chromosome 14. A quantitative trait locus (QTL) on chromosome 17 has been previously identified as a potential modifier locus of dorsal pigmentation in hooded Long-Evans Agouti (LEA) rats. To validate the contribution of this chromosome 17 locus, we analyzed an (LEA × F344) F2 population and identified distinct genetic determinants of dorsal and ventral pigmentation. Genome-wide QTL mapping identified a major locus influencing dorsal pigmentation on chromosome 17 near D17Rat2 exceeding a highly significant level. In contrast, ventral pigmentation showed a distinct genetic pattern from dorsal pigmentation, although no genome-wide significant loci were identified. We established an F344-TyrC line (non-albino hooded F344) and generated congenic rats carrying an LEA-derived chromosome 17 segment on an F344 background. Congenic rats heterozygous for the LEA-derived chromosome 17 segment showed a marked increase in dorsal pigmented coat area compared to hooded F344 rats (55% vs. 40%). Boundary marker analysis refined the interval to 0-6.8 Mbp. Collectively, the findings indicate that the LEA allele on chromosome 17 increases dorsal but not ventral pigmentation even in the heterozygous state. This refinement of the genetic architecture of hooded coat patterns provides a valuable resource for identifying genes responsible for region-specific pigmentation in rats.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of bovine rhinitis A and B viruses in Japan and evidence of viral RNA amplification in primary bovine respiratory epithelial cells. 日本牛鼻炎A和B病毒的遗传特征及原代牛呼吸道上皮细胞中病毒RNA扩增的证据。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-25 DOI: 10.1292/jvms.26-0388

Hiroho Ishida, Kanae Hirata, Miri Akiyama, Mikari Nakamura, Yui Watanuki, Hironobu Murakami, Kei Kazama, Kazuhiro Kawai, Ken Onda, Koji Yoshioka, Sachiko Arai, Yasunori Shinozuka, Kaoru Hori, Takanori Shiga, Mami Oba, Hitoshi Takemae, Tetsuya Mizutani, Sachi Tamaboko, Yuka Yamauchi, Makoto Nagai

{"title":"Genetic characterization of bovine rhinitis A and B viruses in Japan and evidence of viral RNA amplification in primary bovine respiratory epithelial cells.","authors":"Hiroho Ishida, Kanae Hirata, Miri Akiyama, Mikari Nakamura, Yui Watanuki, Hironobu Murakami, Kei Kazama, Kazuhiro Kawai, Ken Onda, Koji Yoshioka, Sachiko Arai, Yasunori Shinozuka, Kaoru Hori, Takanori Shiga, Mami Oba, Hitoshi Takemae, Tetsuya Mizutani, Sachi Tamaboko, Yuka Yamauchi, Makoto Nagai","doi":"10.1292/jvms.26-0388","DOIUrl":"https://doi.org/10.1292/jvms.26-0388","url":null,"abstract":"The aphthoviruses bovine rhinitis A virus (BRAV) and bovine rhinitis B virus (BRBV) can be detected in bovine respiratory specimens. However, information regarding Japanese strains and genome-scale data remains limited. To address this gap, we screened Japanese bovine nasal swab specimens for BRAV and BRBV using reverse transcription-quantitative polymerase chain reaction and subjected positive specimens to genome sequencing. Among 98 specimens from cattle admitted to Azabu University, BRAV and BRBV were detected in 8 each using a cycle threshold (Ct) cutoff ≤35. Near-complete genome sequences were obtained from four BRAV-positive specimens and one BRBV-positive specimen. Phylogenetic analyses revealed that four BRAV strains belonged to a BRAV1-related lineage distinct from the Japanese BRAV2 strain H-1. In contrast, BRBV/25IKH5 clustered with LAV/238002 within the BRBV-1 group. To evaluate viral RNA amplification after passage, specimens with low Ct values that tested positive for BRAV or BRBV alone were inoculated onto Madin-Darby bovine kidney (MDBK) cells and primary bovine respiratory epithelial cells. RNA from BRAV/LAV/238037 and BRBV/25IKH5 was detected in culture supernatants of primary bovine respiratory epithelial cells but not in those of MDBK cells, and there was no apparent cytopathic effect. Following passage in primary cells, mean Ct values decreased from 37.0 to 23.9 for BRAV/LAV/238037 and from 30.7 to 22.7 for BRBV/25IKH5 at 72 hr after inoculation. This study expands genome-scale information on bovine rhinitis viruses and presents evidence of BRAV and BRBV RNA amplification after passage in primary cells.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a rapid and simple evaluation system for disinfectant efficacy against Mycobacterium avium subsp. paratuberculosis using a visual time-to-detection based liquid culture system. 建立一种快速简便的禽分枝杆菌消毒液效果评价体系。副结核菌使用基于视觉检测时间的液体培养系统。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-25 DOI: 10.1292/jvms.26-0347

Ikuyo Ohashi, Satoko Kawaji, Daisuke Takamatsu

引用次数: 0

Computational prescreening of anticancer drugs prior to in vitro validation, primarily in dogs with exploratory cat data, based on cross-species binding conservation. 体外验证前抗癌药物的计算预筛选，主要是在具有探索性猫数据的狗中，基于跨物种结合保护。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-22 DOI: 10.1292/jvms.26-0252

Seoung-Woo Lee, Hee-Yeon Kim, Seong-Kyoon Choi, Su-Geun Lim, Jiwon Ko, JiYeong Park, Su-Min Baek, Jin-Kyu Park, Soyoung Jang, Choonok Kim, Song Park, Jee Eun Han, Wansoo Kim, Sehyeon Han, Sangyeol Kim

{"title":"Computational prescreening of anticancer drugs prior to in vitro validation, primarily in dogs with exploratory cat data, based on cross-species binding conservation.","authors":"Seoung-Woo Lee, Hee-Yeon Kim, Seong-Kyoon Choi, Su-Geun Lim, Jiwon Ko, JiYeong Park, Su-Min Baek, Jin-Kyu Park, Soyoung Jang, Choonok Kim, Song Park, Jee Eun Han, Wansoo Kim, Sehyeon Han, Sangyeol Kim","doi":"10.1292/jvms.26-0252","DOIUrl":"https://doi.org/10.1292/jvms.26-0252","url":null,"abstract":"Advances in veterinary medicine have prolonged the lifespan of companion animals. As a result, the burden of neoplastic diseases has increased. While targeted anticancer drugs are widely used in human oncology, their clinical translation to companion animals remains limited. Therefore, a systematic framework for prioritizing candidate drugs before in vitro or in vivo testing would be a valuable strategy in veterinary medicine. In the present study, we evaluated the cross-species binding potential of FDA-approved targeted anticancer drugs to canine and feline target proteins. Protein sequence analysis revealed high conservation of drug-binding domains between human, dog, and cat target proteins, with average sequence identities of 97.09% in dogs and 99.12% in cats compared with their human counterparts. Computational docking simulations were performed to evaluate the docking scores between selected drugs and their corresponding target proteins in dog and cat. Most selected drug-target complexes exhibited docking scores comparable to those observed for human targets, and cross-species docking-score correlations were high in both dogs (0.988) and cats (0.996). These findings support the use of docking-based prescreening as a rapid candidate-selection strategy prior to in vitro validation in veterinary medicine.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum fibroblast growth factor-23 and magnesium concentrations in cats with acute kidney injury: association with disease pathophysiology. 急性肾损伤猫血清成纤维细胞生长因子-23和镁浓度：与疾病病理生理学的关系

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-22 DOI: 10.1292/jvms.26-0210

Akiko Miyagawa, Mayu Nakano, Hirosumi Miyakawa, Yuichi Miyagawa, Naoyuki Takemura

{"title":"Serum fibroblast growth factor-23 and magnesium concentrations in cats with acute kidney injury: association with disease pathophysiology.","authors":"Akiko Miyagawa, Mayu Nakano, Hirosumi Miyakawa, Yuichi Miyagawa, Naoyuki Takemura","doi":"10.1292/jvms.26-0210","DOIUrl":"https://doi.org/10.1292/jvms.26-0210","url":null,"abstract":"Cats with acute kidney injury (AKI) have a high mortality rate and are at risk of progression to chronic kidney disease (CKD). Fibroblast growth factor-23 (FGF-23) and magnesium, which are involved in mineral homeostasis and AKI outcomes, may contribute to this progression. This study aimed to evaluate serum FGF-23 and magnesium concentrations at diagnosis in cats with AKI and to investigate their associations with clinical outcomes. This retrospective study included client-owned cats with AKI. Serum FGF-23 and magnesium concentrations were measured at diagnosis, and their associations with AKI outcomes were analyzed. Forty-five cats were enrolled. Serum FGF-23 (P=0.01) and magnesium (P=0.01) concentrations were elevated. Serum FGF-23 tended to increase with prior CKD severity, although differences among CKD groups were not significant (P=0.10). Serum FGF-23 was significantly associated with treatment duration (P=0.008) and changes in serum creatinine during hospitalization (P=0.003), independent of prior CKD status and serum creatinine at diagnosis. A 10-fold increase in FGF-23 was associated with an approximately 47% decrease in the hazard of reaching the lowest serum creatinine concentration. Magnesium was not significantly associated with these outcomes. Measurement of serum FGF-23 at the time of AKI diagnosis may help predict hospitalization duration and renal function decline. Although the role of serum magnesium in the pathophysiology of AKI could not be clearly determined, further investigation of its relationship with FGF-23 may contribute to the development of novel therapeutic strategies.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the efficacy of sevoflurane as a general anesthetic method for noninvasive examination of gentoo penguins (Pygoscelis papua). 七氟醚在巴布亚企鹅无创全身麻醉检查中的效果评价。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-21 DOI: 10.1292/jvms.26-0226

Koji Ono, Satoshi Inoue, Hirofumi Hatakeyama, Riko Ito, Toko Maehara, Masahiro Yamasaki, Hiroshi Satoh

{"title":"Evaluation of the efficacy of sevoflurane as a general anesthetic method for noninvasive examination of gentoo penguins (Pygoscelis papua).","authors":"Koji Ono, Satoshi Inoue, Hirofumi Hatakeyama, Riko Ito, Toko Maehara, Masahiro Yamasaki, Hiroshi Satoh","doi":"10.1292/jvms.26-0226","DOIUrl":"https://doi.org/10.1292/jvms.26-0226","url":null,"abstract":"This study evaluated the efficacy of sevoflurane for maintaining general anesthesia during noninvasive examinations, such as computed tomography, in gentoo penguins (Pygoscelis papua). Ten captive penguins were enrolled in a prospective crossover study and assigned to two protocols. In the S group, anesthesia was induced with intravenous alfaxalone and maintained with sevoflurane, titrated to achieve an appropriate depth for noninvasive procedures. Sevoflurane concentration, recovery times, heart rate, and blood pressure were evaluated. In the premedication with midazolam and butorphanol (MBS group), the same protocol was used following premedication with intramuscular midazolam (0.25 mg/kg) and butorphanol (0.25 mg/kg). Blood samples were collected before and after anesthesia to assess lactate, glucose, and blood gas variables. The mean maintenance concentration of sevoflurane was significantly lower in the MBS group (0.5%) than in the S group (1.0%). Time to extubation (2 vs. 4 min) and complete recovery (7 vs. 12 min) were shorter in the MBS group. In both groups, heart rate remained within physiological ranges, and blood pressure was stable. In the S group, preanesthetic lactate was significantly higher, whereas base excess, bicarbonate, and total carbon dioxide were significantly lower than in the MBS group. Overall, alfaxalone induction followed by sevoflurane maintenance provided stable anesthesia and rapid recovery. Premedication with midazolam and butorphanol offered clinical advantages, including reduced sevoflurane requirements, faster recovery, and decreased preanesthetic stress.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxibendazole induces cell cycle arrest and apoptosis in canine large-cell alimentary lymphoma cells. 氧化苯达唑诱导犬大细胞消化道淋巴瘤细胞周期阻滞和细胞凋亡。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-21 DOI: 10.1292/jvms.26-0360

Kosei Sakai, Masaki Hirao, Nana Suzuki, Shunsuke Shimamura

引用次数: 0

Hepatic coccidiosis caused by Eimeria ninakohlyakimovae in a goat (Capra hircus) in Japan. 日本山羊（Capra hircus）因艾美耳球虫引起的肝球虫病。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-18 DOI: 10.1292/jvms.26-0339

Kana Yamaguchi, Makoto Matsubayashi, Jun Nishii, Yuto Takenaka, Chieko Iwamoto, Kotaro Fujii, Kazuhiro Kojima, Tomoyuki Shibahara

引用次数: 0

Molecular survey of the seven recognized Eimeria species in chicken farms in Japan. 日本养鸡场7种艾美耳球虫的分子调查。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2026-09-17 DOI: 10.1292/jvms.26-0416

Guohong Zhang, Erika Tanahashi, Yuuki Morozumi, Ryohei Nukui, Akira Fujiwara, Nobuyuki Tsutsumi

引用次数: 0