Journal of Veterinary Medical Science最新文献_第5页

Magnetic resonance imaging and pathological findings of aortoiliac thromboembolism with a thoracic aortic mural thrombus in a Japanese Black calf.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-21 DOI: 10.1292/jvms.24-0407

Natsuki Akashi, Ayako Okada, Koutaro Nakamura, Takeshi Tsuka

引用次数: 0

Current status of the distribution and antimicrobial resistance of Staphylococcus felis isolates from feline patients in Japan. 日本猫患者猫葡萄球菌分离株分布及耐药性现状

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-03 DOI: 10.1292/jvms.24-0452

Kakeru Izumi, Yuzo Tsuyuki, Kazuki Harada

引用次数: 0

A rare case of solitary intrahepatic splenosis following splenectomy in a dog. 一例罕见的狗脾切除术后单发肝内脾病。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-15 DOI: 10.1292/jvms.24-0080

Hiroshi Sunahara, Yuki Nemoto, Dito Anggoro, Melpa Susanti Purba, Kazuhito Itamoto, Harumichi Itoh, Munekazu Nakaichi, Kenji Tani

{"title":"A rare case of solitary intrahepatic splenosis following splenectomy in a dog.","authors":"Hiroshi Sunahara, Yuki Nemoto, Dito Anggoro, Melpa Susanti Purba, Kazuhito Itamoto, Harumichi Itoh, Munekazu Nakaichi, Kenji Tani","doi":"10.1292/jvms.24-0080","DOIUrl":"10.1292/jvms.24-0080","url":null,"abstract":"A 9-year-old spayed female mixed breed dog weighing 6.8 kg with a history of previous splenectomy for hemangiosarcoma 4 years earlier was referred for a hepatic mass lesion. Although the dog did not have a clinical sign, a computed tomography revealed a solitary mass in the left medial lobe of the liver. Spleen-like tissue was observed on needle core biopsy of the mass, leading to the diagnosis of intrahepatic splenosis. Therefore, hepatic lobectomy of the left medial lobe of the liver was performed. Histopathological evaluation of the excised tumor confirmed the diagnosis of intrahepatic splenosis. No further development of splenosis was detected on CT performed 217 days after the surgery. The dog died of suspected aspiration pneumonia without any event or clinical signs related to intrahepatic splenosis on postoperative 272 days. The cause of the intrahepatic splenosis observed in this dog was unknown; however, hematogeneous dissemination and autoimplantation of splenic tissue via the portal venous flow was speculated. This case shows the utility of needle core biopsy for the preoperative diagnosis of splenosis. The intrahepatic splenosis in this case was considered to have developed as a long-term sequelae after splenectomy, as in humans.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"286-290"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Daily topical application of chlorhexidine gluconate to the skin in dogs and its impact on skin barriers and cytotoxicity. 每日局部应用葡萄糖酸氯己定在狗的皮肤及其对皮肤屏障和细胞毒性的影响。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-16 DOI: 10.1292/jvms.24-0311

Akira Matsuda, Yukari Nakamura, Takamasa Itoi, Kiyotaka Arai, Kenji Kutara, Keisuke Sugimoto, Noritaka Maeta

{"title":"Daily topical application of chlorhexidine gluconate to the skin in dogs and its impact on skin barriers and cytotoxicity.","authors":"Akira Matsuda, Yukari Nakamura, Takamasa Itoi, Kiyotaka Arai, Kenji Kutara, Keisuke Sugimoto, Noritaka Maeta","doi":"10.1292/jvms.24-0311","DOIUrl":"10.1292/jvms.24-0311","url":null,"abstract":"In recent years, the importance of using local disinfectants instead of systemic antibiotics for the treatment of infectious skin diseases to prevent the emergence of resistant bacteria has become widely recognized. Chlorhexidine gluconate (CHG) is commonly used in veterinary antibacterial shampoos; however, the daily topical application of diluted CHG solutions has also been adopted. Despite its widespread use, few studies have investigated the effects of CHG on the canine skin barrier. This study examined the skin barrier-damaging effects of CHG in dogs by applying diluted CHG (0.05%, 0.5%, and 4%) daily to six healthy dogs for 2 weeks. At the end of the trial, transepidermal water loss (TEWL), skin surface hydration (SSH), and number of skin bacteria were evaluated. Additionally, the cytotoxicity of CHG to the canine progenitor epidermal keratinocytes (CPEK) was examined using the water-soluble tetrazolium salt (WST-8) assay. Continuous application of 0.5% and 4% CHG to the skin led to a significant reduction in the number of skin bacteria. Additionally, at 4% CHG, a notable increase in TEWL and a decrease in SSH was observed. The WST assay revealed cytotoxicity of CHG at concentrations of 0.05%. In conclusion, although daily topical application of 4% CHG demonstrated the potential to disrupt the skin barrier, 0.5% CHG demonstrated sufficient antimicrobial activity without skin barrier disruption. Additionally, when treating dogs' skin with a compromised stratum corneum, use of lower concentration of CHG is suitable.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"257-262"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colostomies for two lizards with cloacal prolapse. 结肠造口术治疗2例蜥蜴的肛管脱垂。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-03 DOI: 10.1292/jvms.24-0437

Yoshinori Takami, Yumi Une

引用次数: 0

Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.1292/jvms.24-0296

Natsume Kurahara, Ayami Yutsudo, Osamu Yamato, Noriaki Miyoshi, Tatsuro Hifumi, Akira Yabuki

{"title":"Apoptosis in kidney tissue of senior and geriatric cats with chronic kidney disease.","authors":"Natsume Kurahara, Ayami Yutsudo, Osamu Yamato, Noriaki Miyoshi, Tatsuro Hifumi, Akira Yabuki","doi":"10.1292/jvms.24-0296","DOIUrl":"10.1292/jvms.24-0296","url":null,"abstract":"Apoptosis, an important pathological event associated with kidney disease progression, is expected to be a therapeutic target in chronic kidney disease (CKD). However, its role in naturally occurring CKD in aged cats remains unclear. Therefore, here, we investigated kidney tissues from aged cats (≥10 years) with or without azotemic CKD to evaluate apoptotic events using a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay. The positive TUNEL signals of the renal cells were quantified and statistically analyzed for correlation with the severity of plasma creatinine (pCre) concentration, renal lesions (glomerulosclerosis, interstitial cell infiltration, peritubular capillaries, and interstitial fibrosis), and oxidative damage of the kidney tissue. Oxidative damage was evaluated using immunohistochemistry for 8-hydroxy-2'-deoxyguanosine (OHdG) and 4-hydroxynonenal (HNE). In the TUNEL assay, regardless of azotemia, positive nuclear signals were observed in the tubular epithelial and intraluminal cells, interstitial infiltrating cells, and glomerular cells. Quantitative TUNEL scores showed no significant differences between the azotemic and non-azotemic groups in any compartment of the kidney tissues. In the azotemic group, TUNEL scores did not correlate with pCre or renal lesion severity. However, the scores showed a significant positive correlation with the scores of 8-OHdG and 4-HNE. These findings suggest that apoptosis associated with oxidative damage in renal tissue is an initial pathological event that leads to CKD, rather than a change following CKD progression, in aged cats. Inhibiting apoptosis by antioxidant treatment may be a key strategy to prevent the development of CKD.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"248-256"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective detection of ITGB7 gene mutation in a holstein calf with chronic diarrhea that was suspected of hereditary cholesterol deficiency. 怀疑遗传性胆固醇缺乏的荷斯坦犊牛慢性腹泻患者ITGB7基因突变的回顾性检测

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-15 DOI: 10.1292/jvms.24-0392

Hisashi Inokuma, Masaki Maezawa, Gaku Tahara, Yoshiyuki Miyazaki, Atsushi Ogino, Ken-Ichi Watanabe, Yoshiyasu Kobayashi

引用次数: 0

Depot-specific adiposity changes in ovariectomized mice on high-fat diet.

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.1292/jvms.24-0442

Danang Dwi Cahyadi, Katsuhiko Warita, Yoshinao Z Hosaka

{"title":"Depot-specific adiposity changes in ovariectomized mice on high-fat diet.","authors":"Danang Dwi Cahyadi, Katsuhiko Warita, Yoshinao Z Hosaka","doi":"10.1292/jvms.24-0442","DOIUrl":"10.1292/jvms.24-0442","url":null,"abstract":"Ovariectomized (OVX) mice serve as a key model for studying postmenopausal metabolic changes, particularly obesity, as they mimic the hormonal state of postmenopausal women. However, our understanding remains limited regarding how hormonal and dietary factors affect different adipose tissues. Furthermore, precise documentation of experimental procedures and their effects on specific adipose tissue depots is essential for reproducible translational research. This study investigated depot-specific adiposity development in OVX mice fed a high-fat diet (HFD), focusing on how reduced estrogen levels and dietary intervention affect distinct fat depots. We composed subcutaneous and visceral white adipose tissue (WAT) depots from sham-operated (Sham) and OVX female C57BL/6JJcl mice on a regular diet (RD) and high-fat diet (HFD) for 20 weeks. OVX mice on HFD gained significantly more weight than Sham controls. Adiposity increased in abdominal subcutaneous WAT (sWAT) and perirenal WAT (prWAT) of OVX mice, but not in mesenteric WAT (mWAT). Analysis of adipose tissue morphology revealed that OVX mice exhibited enlarged adipocyte cross-sectional areas under low estrogen (E2) conditions, suggesting enhanced adipogenesis in an estrogen-deficient state. These findings suggest that low estrogen condition accelerated adiposity, in a tissue site-dependent manner.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"241-247"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer potentials of chaga and notoginseng against dog bladder cancer organoids. 对犬膀胱癌类器官有抗癌潜力的白桦和三七。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-15 Epub Date: 2025-01-03 DOI: 10.1292/jvms.24-0258

Yuta Shinohara, Mohamed Elbadawy, Yishan Liu, Megumi Yamanaka, Haru Yamamoto, Yomogi Sato, Amira Augomaa, Yusuke Ishihara, Tatsuya Usui, Kazuaki Sasaki

{"title":"Anticancer potentials of chaga and notoginseng against dog bladder cancer organoids.","authors":"Yuta Shinohara, Mohamed Elbadawy, Yishan Liu, Megumi Yamanaka, Haru Yamamoto, Yomogi Sato, Amira Augomaa, Yusuke Ishihara, Tatsuya Usui, Kazuaki Sasaki","doi":"10.1292/jvms.24-0258","DOIUrl":"10.1292/jvms.24-0258","url":null,"abstract":"Muscle-invasive bladder cancer (MIBC) is a common form of BC in dogs. Adjuvant chemotherapy administration is commonly applied in MIBC cases, but patients sometimes experience treatment failure and recurrence. Therefore, supplements with anticancer properties, such as traditional Chinese medicines (TCMs), are required, and they have been widely used in Japanese human medicine and may be useful in veterinary medicine. Furthermore, organoid cultures can mimic the characteristics of their original tissues, such as self-renewal and organization. We previously established a novel experimental model for MIBC using a dog BC organoid (DBCO) culture. Herein, we examined the antiproliferative effects and mechanisms of 39 substances, consisting of TCMs, TCM supplements, and crude drug extracts, on DBCOs. Among the TCMs, D3 (also known as Shibe-ria), which is a mixture of chaga (Inonotus obliquus) and notoginseng (Panax notoginseng), significantly diminished the cell viability of DBCOs. The expression of BC stem cell markers, CD44 and SOX2, was reduced considerably in the D3-treated DBCOs. Among the components of D3, chaga exerted an antiproliferative effect on DBCO, whereas notoginseng did not. The administration of D3 also significantly reduced the volume of DBCO xenografted tumors in mice in vivo. Overall, D3 may have benefits as a natural anticancer supplement in veterinary medicine.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"232-240"},"PeriodicalIF":1.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth kinetics and population density of a laboratory colony of the poultry red mite (Dermanyssus gallinae) established in Japan. 在日本建立的家禽红螨实验室种群的生长动力学和种群密度。

IF 1.1 4区农林科学

Journal of Veterinary Medical Science Pub Date : 2025-02-15 Epub Date: 2025-01-01 DOI: 10.1292/jvms.24-0343

Takahiro Inoue, Keiko Mizutani, Hanako Kunisada, Danielle Ladzekpo, Kofi Dadzie Kwofie, Yuki Koike, Sana Sasaki, Kei Jimbo, Hayato Kawada, Fusako Mikami, Anisuzzaman, Md Abdul Alim, Makoto Matsubayashi, Tomoo Yoshida, Takashi Furukawa, Takahisa Ueno, Kenji Oda, Naotoshi Tsuji, Takeshi Hatta

{"title":"Growth kinetics and population density of a laboratory colony of the poultry red mite (Dermanyssus gallinae) established in Japan.","authors":"Takahiro Inoue, Keiko Mizutani, Hanako Kunisada, Danielle Ladzekpo, Kofi Dadzie Kwofie, Yuki Koike, Sana Sasaki, Kei Jimbo, Hayato Kawada, Fusako Mikami, Anisuzzaman, Md Abdul Alim, Makoto Matsubayashi, Tomoo Yoshida, Takashi Furukawa, Takahisa Ueno, Kenji Oda, Naotoshi Tsuji, Takeshi Hatta","doi":"10.1292/jvms.24-0343","DOIUrl":"10.1292/jvms.24-0343","url":null,"abstract":"The poultry red mite (PRM), Dermanyssus gallinae, is a hematophagous ectoparasite that significantly threatens the poultry industry, not only through blood-feeding but also as a vector for deadly pathogens. With the growing challenge of acaricidal resistance, the demand for alternative control measures is urgent. However, effective PRM research, particularly in acaricidal efficacy and new drug discovery, hinges on the availability of reliable laboratory colonies. In this study, we successfully established a stable PRM laboratory colony, originally isolated from the field in 2021 and maintained under controlled conditions at the Research Institute for Animal Science in Biochemistry and Toxicology (RIAS). We investigated the growth kinetics and population dynamics of the laboratory colony within a Styrofoam-based maintenance box (SBMB) containing chicks. PRM propagation was tracked over 28 days, with mites collected every seven days. The average bulk weight of the mites in the trap increased from 4.3 ± 1.2 mg on day 7 to 201.4 ± 56.5 mg on day 28, despite seasonal variations, indicating optimal conditions for population growth. The collected mites spanned various blood-feeding developmental stages such as protonymph, deutonymph, and adult stages, enabling comprehensive assessments of molting and egg-laying efficiency. Our findings confirm that the laboratory colony of PRM can be stably maintained, providing a reliable source of PRMs for further experimental research aimed at advancing control strategies against this pervasive pest.","PeriodicalId":49959,"journal":{"name":"Journal of Veterinary Medical Science","volume":" ","pages":"215-222"},"PeriodicalIF":1.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0